LOCUS       DACECB010000001       118746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECB010000001 DACECB010000000
VERSION     DACECB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 118746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.001"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWO04_000001"
     CDS             complement(19..729)
                     /locus_tag="IWO04_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0211647.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWO04_000002"
     CDS             complement(726..2198)
                     /locus_tag="IWO04_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0211648.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWO04_000003"
     CDS             2336..2809
                     /locus_tag="IWO04_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0211649.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWO04_000004"
     CDS             complement(2962..3513)
                     /locus_tag="IWO04_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0211650.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWO04_000005"
     CDS             3618..4148
                     /locus_tag="IWO04_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0211651.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWO04_000006"
     CDS             complement(4631..5074)
                     /locus_tag="IWO04_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0211652.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWO04_000007"
     CDS             complement(5090..6250)
                     /locus_tag="IWO04_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0211653.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWO04_000008"
     CDS             6757..8457
                     /locus_tag="IWO04_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211654.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWO04_000009"
     CDS             complement(8559..9392)
                     /locus_tag="IWO04_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0211655.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWO04_000010"
     CDS             complement(9696..11861)
                     /locus_tag="IWO04_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0211656.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWO04_000011"
     CDS             12121..14415
                     /locus_tag="IWO04_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0211657.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO04_000012"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO04_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0211658.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWO04_000013"
     CDS             15066..16073
                     /locus_tag="IWO04_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0211659.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWO04_000014"
     CDS             16095..17114
                     /locus_tag="IWO04_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0211660.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO04_000015"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO04_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0211661.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IWO04_000016"
     CDS             complement(18871..20292)
                     /locus_tag="IWO04_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0211662.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO04_000017"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO04_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0211663.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IWO04_000018"
     CDS             complement(22703..23959)
                     /locus_tag="IWO04_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0211664.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IWO04_000019"
     CDS             24539..25840
                     /locus_tag="IWO04_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0211665.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IWO04_000020"
     CDS             complement(25909..26508)
                     /locus_tag="IWO04_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0211666.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26763..27644
                     /locus_tag="IWO04_000021"
     CDS             26763..27644
                     /locus_tag="IWO04_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211667.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27657..29267
                     /locus_tag="IWO04_000022"
     CDS             27657..29267
                     /locus_tag="IWO04_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0211668.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29344..30003)
                     /locus_tag="IWO04_000023"
     CDS             complement(29344..30003)
                     /locus_tag="IWO04_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0211669.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30286..30951)
                     /locus_tag="IWO04_000024"
     CDS             complement(30286..30951)
                     /locus_tag="IWO04_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211670.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWO04_000025"
     CDS             complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWO04_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0211671.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWO04_000026"
     CDS             complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWO04_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0211672.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWO04_000027"
     CDS             complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWO04_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0211673.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33265..33585
                     /locus_tag="IWO04_000028"
     CDS             33265..33585
                     /locus_tag="IWO04_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0211674.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33697..34566)
                     /locus_tag="IWO04_000029"
     CDS             complement(33697..34566)
                     /locus_tag="IWO04_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0211675.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34662..35966)
                     /locus_tag="IWO04_000030"
     CDS             complement(34662..35966)
                     /locus_tag="IWO04_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0211676.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35963..36472)
                     /locus_tag="IWO04_000031"
     CDS             complement(35963..36472)
                     /locus_tag="IWO04_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211677.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36647..38782)
                     /locus_tag="IWO04_000032"
     CDS             complement(36647..38782)
                     /locus_tag="IWO04_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0211678.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38865..40520)
                     /locus_tag="IWO04_000033"
     CDS             complement(38865..40520)
                     /locus_tag="IWO04_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0211679.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40522..40836)
                     /locus_tag="IWO04_000034"
     CDS             complement(40522..40836)
                     /locus_tag="IWO04_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0211680.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41006..41266
                     /locus_tag="IWO04_000035"
     CDS             41006..41266
                     /locus_tag="IWO04_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0211681.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41280..41594
                     /locus_tag="IWO04_000036"
     CDS             41280..41594
                     /locus_tag="IWO04_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0211682.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42324..42878)
                     /locus_tag="IWO04_000037"
     CDS             complement(42324..42878)
                     /locus_tag="IWO04_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0211683.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43004..44933)
                     /locus_tag="IWO04_000038"
                     /pseudo
     CDS             complement(43004..44933)
                     /locus_tag="IWO04_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45239..45955)
                     /locus_tag="IWO04_000039"
     CDS             complement(45239..45955)
                     /locus_tag="IWO04_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0211684.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45956..47779)
                     /gene="glpO"
                     /locus_tag="IWO04_000040"
     CDS             complement(45956..47779)
                     /gene="glpO"
                     /locus_tag="IWO04_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0211685.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47793..49289)
                     /gene="glpK"
                     /locus_tag="IWO04_000041"
     CDS             complement(47793..49289)
                     /gene="glpK"
                     /locus_tag="IWO04_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0211686.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49425..50885)
                     /locus_tag="IWO04_000042"
     CDS             complement(49425..50885)
                     /locus_tag="IWO04_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0211687.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50875..52170)
                     /locus_tag="IWO04_000043"
     CDS             complement(50875..52170)
                     /locus_tag="IWO04_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0211688.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52502..53179)
                     /locus_tag="IWO04_000044"
     CDS             complement(52502..53179)
                     /locus_tag="IWO04_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0211689.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53209..54300)
                     /locus_tag="IWO04_000045"
     CDS             complement(53209..54300)
                     /locus_tag="IWO04_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0211690.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54401..54835)
                     /locus_tag="IWO04_000046"
     CDS             complement(54401..54835)
                     /locus_tag="IWO04_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0211691.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55419..56876)
                     /gene="ascB"
                     /locus_tag="IWO04_000047"
     CDS             complement(55419..56876)
                     /gene="ascB"
                     /locus_tag="IWO04_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0211692.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56873..58768)
                     /locus_tag="IWO04_000048"
     CDS             complement(56873..58768)
                     /locus_tag="IWO04_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0211693.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58826..60601)
                     /locus_tag="IWO04_000049"
     CDS             complement(58826..60601)
                     /locus_tag="IWO04_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0211694.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60651..62147)
                     /locus_tag="IWO04_000050"
     CDS             complement(60651..62147)
                     /locus_tag="IWO04_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0211695.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62341..63963)
                     /locus_tag="IWO04_000051"
     CDS             complement(62341..63963)
                     /locus_tag="IWO04_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0211696.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64291..65604)
                     /locus_tag="IWO04_000052"
     CDS             complement(64291..65604)
                     /locus_tag="IWO04_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0211697.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65849..66073)
                     /locus_tag="IWO04_000053"
     CDS             complement(65849..66073)
                     /locus_tag="IWO04_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211698.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66093..66461)
                     /locus_tag="IWO04_000054"
     CDS             complement(66093..66461)
                     /locus_tag="IWO04_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0211699.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(66657..67367)
                     /locus_tag="IWO04_000055"
     CDS             complement(66657..67367)
                     /locus_tag="IWO04_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211700.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67385..67882)
                     /locus_tag="IWO04_000056"
     CDS             complement(67385..67882)
                     /locus_tag="IWO04_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0211701.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67919..68371)
                     /locus_tag="IWO04_000057"
     CDS             complement(67919..68371)
                     /locus_tag="IWO04_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0211702.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68506..69111)
                     /locus_tag="IWO04_000058"
     CDS             complement(68506..69111)
                     /locus_tag="IWO04_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0211703.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69561..70181)
                     /locus_tag="IWO04_000059"
     CDS             complement(69561..70181)
                     /locus_tag="IWO04_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0211704.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70183..71052)
                     /locus_tag="IWO04_000060"
     CDS             complement(70183..71052)
                     /locus_tag="IWO04_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0211705.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71075..71482)
                     /locus_tag="IWO04_000061"
     CDS             complement(71075..71482)
                     /locus_tag="IWO04_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0211706.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71501..72790)
                     /locus_tag="IWO04_000062"
     CDS             complement(71501..72790)
                     /locus_tag="IWO04_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0211707.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72817..73119)
                     /locus_tag="IWO04_000063"
     CDS             complement(72817..73119)
                     /locus_tag="IWO04_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0211708.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73144..75237)
                     /locus_tag="IWO04_000064"
     CDS             complement(73144..75237)
                     /locus_tag="IWO04_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0211709.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75726..76490)
                     /locus_tag="IWO04_000065"
     CDS             complement(75726..76490)
                     /locus_tag="IWO04_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0211710.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76601..77470)
                     /gene="mdcB"
                     /locus_tag="IWO04_000066"
     CDS             complement(76601..77470)
                     /gene="mdcB"
                     /locus_tag="IWO04_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0211711.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77495..78571)
                     /locus_tag="IWO04_000067"
     CDS             complement(77495..78571)
                     /locus_tag="IWO04_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0211712.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78595..79188)
                     /locus_tag="IWO04_000068"
     CDS             complement(78595..79188)
                     /locus_tag="IWO04_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0211713.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79192..80868)
                     /gene="mdcD"
                     /locus_tag="IWO04_000069"
     CDS             complement(79192..80868)
                     /gene="mdcD"
                     /locus_tag="IWO04_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0211714.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80858..81157)
                     /locus_tag="IWO04_000070"
     CDS             complement(80858..81157)
                     /locus_tag="IWO04_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0211715.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81259..82905)
                     /gene="mdcA"
                     /locus_tag="IWO04_000071"
     CDS             complement(81259..82905)
                     /gene="mdcA"
                     /locus_tag="IWO04_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0211716.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82912..83823)
                     /locus_tag="IWO04_000072"
     CDS             complement(82912..83823)
                     /locus_tag="IWO04_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0211717.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83943..84800
                     /locus_tag="IWO04_000073"
     CDS             83943..84800
                     /locus_tag="IWO04_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0211718.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(84986..85348)
                     /locus_tag="IWO04_000074"
     CDS             complement(84986..85348)
                     /locus_tag="IWO04_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211719.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            85694..87133
                     /locus_tag="IWO04_000075"
     CDS             85694..87133
                     /locus_tag="IWO04_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0211720.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87140..89692)
                     /locus_tag="IWO04_000076"
     CDS             complement(87140..89692)
                     /locus_tag="IWO04_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0211721.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89806..91137)
                     /locus_tag="IWO04_000077"
     CDS             complement(89806..91137)
                     /locus_tag="IWO04_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0211722.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91103..92581)
                     /locus_tag="IWO04_000078"
     CDS             complement(91103..92581)
                     /locus_tag="IWO04_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0211723.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(92886..94721)
                     /gene="tdc"
                     /locus_tag="IWO04_000079"
     CDS             complement(92886..94721)
                     /gene="tdc"
                     /locus_tag="IWO04_000079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0211724.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            95191..96618
                     /locus_tag="IWO04_000080"
     CDS             95191..96618
                     /locus_tag="IWO04_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0211725.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(96710..97075)
                     /locus_tag="IWO04_000081"
     CDS             complement(96710..97075)
                     /locus_tag="IWO04_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0211726.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(97072..97437)
                     /locus_tag="IWO04_000082"
     CDS             complement(97072..97437)
                     /locus_tag="IWO04_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0211727.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(97629..97877)
                     /locus_tag="IWO04_000083"
     CDS             complement(97629..97877)
                     /locus_tag="IWO04_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211728.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            97876..98004
                     /locus_tag="IWO04_000084"
     CDS             97876..98004
                     /locus_tag="IWO04_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0211729.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            98061..99755
                     /locus_tag="IWO04_000085"
     CDS             98061..99755
                     /locus_tag="IWO04_000085"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0211730.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            99759..99878
                     /locus_tag="IWO04_000086"
                     /pseudo
     CDS             99759..99878
                     /locus_tag="IWO04_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            99960..100151
                     /locus_tag="IWO04_000087"
     CDS             99960..100151
                     /locus_tag="IWO04_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211731.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            100529..101662
                     /locus_tag="IWO04_000088"
     CDS             100529..101662
                     /locus_tag="IWO04_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0211732.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            101693..102013
                     /locus_tag="IWO04_000089"
     CDS             101693..102013
                     /locus_tag="IWO04_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211733.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(102042..102506)
                     /locus_tag="IWO04_000090"
     CDS             complement(102042..102506)
                     /locus_tag="IWO04_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0211734.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(102637..103890)
                     /locus_tag="IWO04_000091"
     CDS             complement(102637..103890)
                     /locus_tag="IWO04_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0211735.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(103919..104236)
                     /locus_tag="IWO04_000092"
     CDS             complement(103919..104236)
                     /locus_tag="IWO04_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0211736.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(104262..104582)
                     /locus_tag="IWO04_000093"
     CDS             complement(104262..104582)
                     /locus_tag="IWO04_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0211737.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(105046..106461)
                     /locus_tag="IWO04_000094"
     CDS             complement(105046..106461)
                     /locus_tag="IWO04_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0211738.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(106478..107794)
                     /locus_tag="IWO04_000095"
     CDS             complement(106478..107794)
                     /locus_tag="IWO04_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0211739.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(107944..108642)
                     /locus_tag="IWO04_000096"
     CDS             complement(107944..108642)
                     /locus_tag="IWO04_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0211740.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(108931..109924)
                     /locus_tag="IWO04_000097"
                     /pseudo
     CDS             complement(108931..109924)
                     /locus_tag="IWO04_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(110005..110838)
                     /gene="msrA"
                     /locus_tag="IWO04_000098"
     CDS             complement(110005..110838)
                     /gene="msrA"
                     /locus_tag="IWO04_000098"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0211741.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(111072..111815)
                     /gene="truA"
                     /locus_tag="IWO04_000099"
     CDS             complement(111072..111815)
                     /gene="truA"
                     /locus_tag="IWO04_000099"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0211742.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(112006..112641)
                     /locus_tag="IWO04_000100"
     CDS             complement(112006..112641)
                     /locus_tag="IWO04_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0211743.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(112788..114029)
                     /gene="sstT"
                     /locus_tag="IWO04_000101"
     CDS             complement(112788..114029)
                     /gene="sstT"
                     /locus_tag="IWO04_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0211744.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(114350..115027)
                     /locus_tag="IWO04_000102"
     CDS             complement(114350..115027)
                     /locus_tag="IWO04_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0211745.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNSDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(115278..115820)
                     /locus_tag="IWO04_000103"
     CDS             complement(115278..115820)
                     /locus_tag="IWO04_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0211746.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(115825..116052)
                     /locus_tag="IWO04_000104"
     CDS             complement(115825..116052)
                     /locus_tag="IWO04_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0211747.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(116131..118011)
                     /locus_tag="IWO04_000105"
     CDS             complement(116131..118011)
                     /locus_tag="IWO04_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0211748.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            118297..118632
                     /locus_tag="IWO04_000106"
     CDS             118297..118632
                     /locus_tag="IWO04_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0211749.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        34930 a        24912 c        20448 g        38456 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttaca tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaaagt tttataaaaa ggtataaaac
    26701 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
    26761 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
    26821 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
    26881 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
    26941 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
    27001 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
    27061 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
    27121 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
    27181 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    27241 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    27301 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    27361 tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt
    27421 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    27481 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    27541 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    27601 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    27661 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    27721 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    27781 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    27841 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    27901 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    27961 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    28021 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    28081 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat tcgatcactg
    28141 caaaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    28201 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    28261 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    28321 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    28381 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    28441 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    28501 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    28561 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    28621 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    28681 ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat ttgtctagct gctattttac
    28741 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    28801 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    28861 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    28921 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    28981 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg
    29041 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    29101 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    29161 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    29221 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    29281 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    29341 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    29401 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    29461 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    29521 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    29581 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    29641 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    29701 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    29761 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    29821 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    29881 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    29941 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    30001 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    30061 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    30121 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    30181 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    30241 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    30301 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    30361 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    30421 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    30481 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    30541 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    30601 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    30661 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    30721 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    30781 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    30841 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    30901 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    30961 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    31021 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    31081 ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg
    31141 gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct
    31201 cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt
    31261 gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta
    31321 tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg
    31381 cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg
    31441 aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag
    31501 aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa
    31561 gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga
    31621 tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg
    31681 atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa
    31741 atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt
    31801 gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact
    31861 acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc
    31921 atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag
    31981 taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt
    32041 tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct
    32101 agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc
    32161 gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg
    32221 tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt
    32281 ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact
    32341 tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta
    32401 taattcttaa cgatcagcaa aactcctttt ccactatcgg cttccttaat agccgtctga
    32461 atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct
    32521 tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca
    32581 acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt
    32641 tctggatagc tttttactaa tccttccagc atttcttcga caatattgct tggatcattc
    32701 atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat
    32761 ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa
    32821 aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag
    32881 ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct
    32941 tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc
    33001 gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat
    33061 accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt
    33121 cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta
    33181 tataataaat ttctaatttt gggaaaatat gtgtagtgct tcttgaagtt tgttatactt
    33241 taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa
    33301 gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt
    33361 aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac
    33421 gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta
    33481 ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc
    33541 atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt
    33601 tttaataact aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct
    33661 aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc
    33721 gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt
    33781 ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac
    33841 tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt
    33901 tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt
    33961 ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt
    34021 ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt
    34081 tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc
    34141 ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa
    34201 atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc
    34261 ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt
    34321 agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg
    34381 tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatggt
    34441 tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa
    34501 gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt
    34561 tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc
    34621 ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc
    34681 gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc
    34741 gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga
    34801 aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat
    34861 cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt
    34921 tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata
    34981 caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca
    35041 atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca
    35101 ccttttgccc aacgttttct gtgtcgatat agcatcttca acgtcacagg tacttccatg
    35161 aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca
    35221 atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct
    35281 tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt
    35341 gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat
    35401 ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg
    35461 cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca
    35521 ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt
    35581 cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct
    35641 aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga
    35701 taattcaatt tcgtcatcaa atactcgatt gtatattcga tcacaatttc ttcattatgg
    35761 gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt
    35821 tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct
    35881 aaaatcggat aagaaacgag aaaaaaatac ataatagaca aacaagtatc taagaataaa
    35941 ttacctgtat gggtgattga gatcatttca gttccacccc attttcttta tacaaatctg
    36001 caatgaatgt cgtatctaga tttttctctt cggagacaga ataaaaacgg accgtttctc
    36061 tctcttcttt tgttccaaaa cgttccgtat acacttcttc aagtaattct tgtctttttt
    36121 tcaatttttc atcttcatat tcaaaagatt tatttacttt tttgtataga tttcgattgt
    36181 tccacctagt caatacaaat gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc
    36241 cgattaagaa atacagcatt ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg
    36301 ctctttctgg tctcgttaat gggaaaaaga cccatataaa aggaagaaca acgatcatcc
    36361 atcctaaaag agccactaac gtttgccaaa tctttaacca gtaattacct tttgaaaaat
    36421 acttatcttt caccaaaatt gatgcaactt cttctcttgt tacttttttc atatcctcac
    36481 ctcacgtttt tttgttacga cgtaggagtt taccatacat ttcatttctt ttcacataaa
    36541 gtgttcctaa atttttccta agaaaaagaa caaaacattt ctcatcagca aatgaaaaat
    36601 attttgttct ttttatcagt tgttcttttt attttttatc tcttaattat gttgctcttt
    36661 ccaatacgac aatgcttttc attcctctag atatcgccgt gtacatttgt ctgcgtcttc
    36721 tttgctcatc tccatcgttt tcaaatggat ataagattac atgatcaaat tccagtcctt
    36781 ttgctagact caaaggaatg atttggcagt tttctaatcc atatgaattt tttttaatcg
    36841 attcttgtag cgtttgggtt tcctcttctg tttgggtgat aactgccgct ttttctcctt
    36901 ttagagaaac caaaatctca gctagtattt tcaacaattc ttcagaggat cgaaaagagc
    36961 ggtattcggg ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca
    37021 cagcataggt tttgaaaagc tctgtgattg ctccgctaga acggtaacta tttctaagat
    37081 cataccgatg gatcggcaga tggtagtcgt cgaaacaacg cataatatca gcaaaagtag
    37141 tggaagaatt aaagattgcc tgattctcgt cacctactaa agtaaagtga gtttttggaa
    37201 ataattcgat caatagacct aattgtgcca gggtataatc ttgtacttca tcaatcaata
    37261 gatagcgaaa tgctggtaca tttacctttt caacaagtaa atgacgaata gctaatataa
    37321 tgactgcttc atcaacagta cgtgtgggtt gccaagcata agctgagcca tatcgttttt
    37381 cataaattct ttcaaacaac tgatgctcat ccacccatgc catttcttcg atttgtctgg
    37441 ttatttttcg atattttttt tgtaaaagtt tccttgcata agctacaata ctttgttccg
    37501 aatcatcaga aatcaattta ccgaataatt ccaactgcct atcttctgtc aacgagagta
    37561 cctgattctg cacagcagga ctttttgctt gtttcaaaag atgattcttc cattcgctca
    37621 tcagtgcctt tttcgttgct tgaagcctat cgatcaattt aggatgagca ggaactttct
    37681 catatatttt ttcgatcgtt tttttagaaa ggactgtttt tccattttta gtaagtccac
    37741 ggataaaatt cggatgatct aaaaacaaag gatctgattg ttttagatta tcgataaact
    37801 ttttgctacg caaacgttct gtctgctccg atacattttc tcccgtgata cgtttgaaat
    37861 aagcctcttc accttctatt tcctctgctg aaagctgtga aaccagctgt atgatggtta
    37921 gattcagtgg atttctttca cctaatgatg gtaggacatt cgaaatatag tcgataaacc
    37981 gatgattagg tgatagaatc agcaagtcat cggatgtcat cgtatctcga tataaataga
    38041 gcagatacgc aatacgctgc atgatagcag aggttttacc acttcctgct acgccattta
    38101 ccaagatatt ttgacttttc acatcccgaa tgattgtgtt ttgttccgat tggatcgtag
    38161 cagtgatagc cttcatttca ttggtctcat cttggcctaa gacttctaat aatacgtcat
    38221 cttgaatagc tacagacgta tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt
    38281 gtcgtctttc tttaataacc gtatcgattt tattttgacg aaccgtataa gaagatggac
    38341 caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa acaagatttt
    38401 catctgcatc atctgtgaaa tttgctgtac cgatataaaa ggattctttt ggctcattat
    38461 ccagaaagtc tacaacgact tttccaaagt agggactttt caataaacgt tcgatctttg
    38521 ctagctgatt ttctgcgttt ttctgtttga tattcatctg atcgatttct ctatttttca
    38581 tttcaatcat cgaaaaagta tccaggttat ctaagtaact gtcaaaattc aatcgaagct
    38641 cgccagccat ttctttcaac gactggtttc cttctatttt tgccgtatcc atcgattccc
    38701 gcagttcgtt ttttttatca attagctttt gatacacgtt cttcagatat gcttcttcta
    38761 actgtttttg tgtgttgacc atacacattc tcctcttttt ttaaagtggt ggaaccgatt
    38821 agtttacctc ttttaagagt cgaatgcaag ctttttaacg agtatcattt gttaggatat
    38881 atcagttctt caggtcggac agtttgatat aaagaaaatg caccatcaag gttttttacg
    38941 tggtagccag actgcttcaa gattctttcc gctagatagc tacgtaatcc gctatgacag
    39001 ctgacgatat attcttgttg gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa
    39061 gggatatgtt tggcttcctt gaatttgccc ttttccaatt cttctgctgt tcgcacatct
    39121 aataaaattt ttccttttgc tagttctgtc ggcaattcat gccattgtac ggtttcacta
    39181 agcccttctg caatgttcat tgctgcatat cctagcatgt ttactggatc tttggctgag
    39241 ccaaatggcg gtgcatacgt caattctaat tccggtaaat cgaagatcgt caaatgccct
    39301 ttgattgctg ttgctaaaat atcgatccgt ttatccacac cttttgcccc aacaccttgt
    39361 gcaccataaa tctcgccagt ctctggatgg aagatcaact tcagtaaaat atctgttgct
    39421 tctggataat agcttgcatg atcttttcct gatacatgaa tgacttcaaa agacagttcg
    39481 ttttctttgg ctgttctttc acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt
    39541 ataattgctg ttccaatacc gccttgattt cttcgagaaa ttccggaaat cgtgtccgca
    39601 acttgtctac cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg
    39661 atttgctgtt ttacaacgat agcatccccc accgcataaa tatcaggaat attcgtttga
    39721 taacgttcgt ctactaggat acccccgcgt atgcctaatt cgattccagc ggtcactgct
    39781 agctggttat caggacgaac accaatggat aaaataacta aatcagcagg gatggtacgc
    39841 ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc atgagaagta
    39901 atcacttcta ctccttgatg agacaattct ttttctataa aagctgccat ttcttcatcc
    39961 agtgttggaa gaatatgtgg tgcttgttcg atcaccatga cttctaaacc tcttcttttt
    40021 aggttttctg ccatttccaa tccgatgaat cctgcaccaa cgatcacggc acgctttggc
    40081 tgtttttcta atgcatgcat cactttatcg atatctggta catttctaat agaaaaaaca
    40141 ttgtcggctt ctgttatccc tggaatcgat ggaacaactg gttttgcacc aggagataaa
    40201 accaatgcat cgtactgttc aatgtgtttt tgaccctcat gtacgacttc taccgttttg
    40261 ttttcgggaa agatggctgt cacttcatga tttggacgca catctaaaca aaaacgagcg
    40321 gataatgatt ctggcgtttg gacaagaaga ttttcccgtt cactgatttc tcccgaaaga
    40381 taataaggca agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt
    40441 tctgcatctt ccattaatcg gcgaagccgt gtagcagcag acattccacc tgctacacct
    40501 ccgacaataa cgattttcat cttatttccc tcctactgtt cgatcaaacc attgattcat
    40561 gccaccacgt acattcacta catcataacc agatttcttt aattctttag ctgcttgttt
    40621 actcctcatc cctgattgac aaatcacata gacggtcttt tctttgtctc catcatatct
    40681 gtttatactt tgtaatggaa catttttagc tccttttata tgcccgcccc ggtattctgc
    40741 aggtgttcta acatcaagta acgctggatt ttccaaaagc ttttccttta attctgggac
    40801 tgttatcgat tttattgatt gaaatagcaa attcattttc tgcacctcat ttacattttt
    40861 ataaatacct catggggtat attaaacagg agctttatta atatgtcaac cagaatgact
    40921 cttcattttt ttcactactt tttatatttg cataaaatac cgataagggg tattatctaa
    40981 ttataaaaat agaggagaat gtgttatgga gattgataat aaaaatatcc tgaaccgttt
    41041 gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg
    41101 tatggatgtc atcactcaat taagcgctgt tcgttctagt attgatcgcg taatgggcat
    41161 gatcgttgca gataatctga aaaattgctt tgagaaacct gatagtaatc cagaagaaca
    41221 agcggctaaa ctagaacaag cgatcaagat gattattaaa aagtaaagga atgatttcaa
    41281 tgaaagtcgt tttccatatt gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa
    41341 aaaacctgat aaaagcttct ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga
    41401 ttaccggtta tctagactct gccaatgcag aattcttaga tttgcaagga gtcgtttttc
    41461 acgcttgcgc caatgctatg cgtgctaatc atatttcaga aagttcttta cctgaacaag
    41521 taatcgttgt accagcaggt gtccttgatt tagttgaact gcaatcccaa ggttatgctt
    41581 atatcaagcc ttagtcagta gaggttgcga cagaagtgat cagcttcaag aaatttcggc
    41641 ttatttttcg aagaagctac ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc
    41701 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggttg
    41761 ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag
    41821 cggtttgacc taagtattag agaaaaaaac gggaaaatag ctcctcatat tttgccgttt
    41881 tttgagctaa taccgaaggt cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa
    41941 gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac atttttgcat
    42001 aaatttggct taataccgag aaactggctt ttgaataccg tttaaggaag aggttgtaaa
    42061 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagctgct catattttgc
    42121 cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaccaa gaggttgtga
    42181 gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacaacttct cctgtttttg
    42241 gttcggttgg cttattatcg agaagtagct actgggagtc tatttatcaa aaaaaggaat
    42301 gcgacatagg tcacacccct tttttatttt ctttgaaaca tctttcggta ctcgacagca
    42361 tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg caaaaatgac tctcacccac
    42421 agcgttggta attctgggaa aagcagcaaa aggatcatgc caacatagat gacagcagtc
    42481 gctactttgc cataccactt cgctccactt aatttgatat ttttagatag cccgattaat
    42541 ccagctacaa acataaagcc ttcttttatc aagaatagac caaacaacag ccaaagccac
    42601 ggtcgatacc atgccataga caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga
    42661 tcaagcacct tccctaagtc tgttacctga ttatatctcc gagcaatgac cccgtctaga
    42721 taatcagaca aacctgagac aacaatgacc gctcctgcta tataaaatga ctgtctcagc
    42781 acaaaataaa gataaatcgg cagcaaaaaa atacggaata tcgataaaag attcggaatc
    42841 gtttgtattt ctttacgcca gtctgtgctt aatttcattt tatcgcccta cctcctcagc
    42901 actcttttct ttttatttta gcttacacgg ggaataaaac caatgatttc actgttttta
    42961 tttttcttta cagacactga tattatgagc taatttgaga taatcacttg ctgttttcat
    43021 cgtttactct tctttcttcc tgtcgtttgc gcatttcttt tactagtact ttatgagctt
    43081 tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt tttataatcg ataaacagac
    43141 tgtctaaatc gtaacctgtc gggtacagat cttctgctga acgcaccaga cgaacacgct
    43201 gacgaggcac gtggattatt tttttgtcta aatatacatc aatcgtatct tcacctatat
    43261 cttcataaac taacgcttct ttttggtatt cattgacaaa tatccgatca ccttttgcaa
    43321 agagctgacg ttctttttta ttctccttcc tatttgtaac tgctgtctta ggaagaaatt
    43381 cttttttctg tgtccgatag gctttttccg ccaagtaacg ctctgcttga tggatcaatt
    43441 tcatcgacat atccatttta tgagcaatcc acaaagcttg gctttctcct gtttcttcca
    43501 acccgcaact gatatttagg acgaagtgct tcccgatcga aagccatagc agccggcaca
    43561 aaatcttcat gatctccggc gaatttcttg atttctccat aatgggtcgt ggcaatgatc
    43621 aatgcccctt gctcatacat agattccatg attgcgatcg ctagtgcagc cccttcattt
    43681 ggctctgttc cactgccaat ctcatctaaa agtaccaacg tattcctttt tatctttttc
    43741 aaaatagcag cgatgttttg catatgacca gaaaatgtac tcaacgcatt ttctaaattt
    43801 tggtggtcac cgacatcgac gaaaatctca tcaaagacag caagctctgt cccttctttt
    43861 gccggaactt gcataccgaa catcgccatc aaagtcaata aaccgactgt cttcaacacg
    43921 actgttttgc ctccggcatt tgctccggtg ataatcaagc ctcgatagtc tttaccgatt
    43981 tcaacatcca atggtaccgc atgctcaggt aaaaatggat gtctgccttg cttgataatg
    44041 atatgttccg atttattgat tctaggtgtg cttccattga tttctcggct aaattttgcc
    44101 cgagcaaaga tgatatctaa caccgtgatc gtttcgatca ataaatcgat cacttcttcg
    44161 ttttctgcga tagcaccagt caatgccgct aggacttgat attcttcact gatttcttca
    44221 gcttttaaca actgatagtg ttcattcaat ttagaaatca cagttggttc aataaaaacc
    44281 gtcgttcttt tatttgattc atcgataatc gttccatcta ttttattttt atagctggct
    44341 ttgataggaa tcgtatagta ttcgcctttt tgaacaatca ttgcttcttg gatcatttct
    44401 ttgtttttcg gatgacgcaa gaatttcagt aattttgact ggatttcttt ttcgattagt
    44461 tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt cgtcactgac tttattattt
    44521 tttattttct ggtcaatcaa ctcttctaca tttatcaaat caggaagatg tttgctataa
    44581 gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa actttgtgat catccgactg
    44641 cttctaagaa aatccgcata ttcgatcaaa tcggttggct gcagaatcaa cccttttttg
    44701 acttgttctg tcagtgcatc gatcctcggc aaccccataa atggaacatg ctgactgctt
    44761 tctaagatca agcgtgcttc tcttgtttct tcttgacgat ccaagacagt ttgtaggttt
    44821 gtcgagaccg tcatatcgct gattcgtttt ttgctatagt ttccaattgc tcttgcttgg
    44881 atttcctttt tgatccgatc aaattgtaat tgactaatca tttcgttgtc catattctct
    44941 ccttttcttg aagagatacc tgtcccaaaa taaataaggg cctagagata aataaaagga
    45001 tagaacacag cttaaaaaag tcatgtccta tccttattag aatacaaaga tattgttttc
    45061 ctgacaggta ccgtttcata agcacaccaa ataaactttg tttattggct attcgactta
    45121 ctgattagat accagctaca ctcacaaaac ataccccatt cttctaggtc ttatttattt
    45181 tccacttaac tatagcaaga cacattttgg ttgtcaaact ttagtatgtt ttatagattt
    45241 acaccattaa tgaatagaca cctaccgcga taattgctcc gatcattgga cctgcgattg
    45301 gtacccatga atatgcccaa tctgaagttc ctttattagc tattggcaat accgcatgtg
    45361 ctaatcgagg accaaagtcc cgagcagggt tgattgcata tcctgttggc cctcctaatg
    45421 aaagaccgat ggctaagatc aatccaccta cagcgaatac gttcgttcca ggtgcaaatt
    45481 cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa tgtaccaatt aattctgtga
    45541 tcacatttgc tggataattc cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc
    45601 tttcatcttt cgttgcattc caatgtggca aataagaaag ccaaacaaga atggcccctg
    45661 caaaagctcc taaaacttgc gcaatgatga aaggaacgac aagattccaa ctaattgccc
    45721 ctgtcatcgc catagctaga gagactgcag gattcaaatg agctgggccc atgaatccag
    45781 aaatatagac agccattgtt acagcgagtc cccaaccaaa tgcgatgaca atccagccag
    45841 aagcctgtgc tttacttttg tttaaattca cagccgcaca cacaccatct ccaagcaaaa
    45901 ctaggatcag tgtcccaata aactccccca tgatttgagt catatcactg ttcatttatt
    45961 tctccccctt tagttcccgc agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct
    46021 gttgcgcctt ctcctcttct gtccattcaa aatatccagc aattgcatcg atcacaggtt
    46081 gcttgatctc atcaagctga tctcgttcaa acaacagatg atttgtacga cgaatgagat
    46141 aatcgcctgg ggccaaaacc atttcttctt ctaaaccata acgtaaccga gctgactcag
    46201 cgagactcaa accaggataa ggtgccattt cttttgctaa ttcaaagata cgtcgggcat
    46261 tagtcccgta aaaatcagcg atataagtag catcttcttc ctctaaaccg gctgctactc
    46321 cttcttttgc taattctgtc actgtttctt ctaatttagt tggatcgaag tttccacctg
    46381 aaatctgata ttttttagag tcgatttctt tcgtctctat tccgtattct tcttttaata
    46441 attggcgtat caatcgcaaa gctccttctg ccatcttacg gtaatcggtg attttcccac
    46501 cagataaagt cagcaaacca tctggttctc tttctaaaga actacctctg gaaatcgtag
    46561 aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa cacgtcttct acttcagcac
    46621 gagaaacttt gttttcctta tattcactta ctgtatcgac aactttattg aagctcttgt
    46681 ctgaaatcga tccattatct ccaccattgt aatcagaacc agaattacca atcaacagtg
    46741 gacgaagtcc tgcccagctt gcttcgatat cattcaatgt gatatttgct tctggatagc
    46801 gatgattgat cacatccaat agataatcta catcttcttg tgtgactttc ggatccgtaa
    46861 aatctccttg gtaatccgta tctgtcgtac caaagtaagt cttgttttct cttggaatag
    46921 caaaaaccat ccgcccatct tgctttcctg tatcgaagta tgtgggttgc ggtacaggca
    46981 gttttttcgc atctacaact aaatggatcc ctttagttgg acgcatttta ggagagactg
    47041 gacgcgtaaa attcaagttt cgtactttat ctacccaagg accactcgta ttgatcacta
    47101 attttgcttt gatttcgatc acttcatctg tcagcaggtc acgagctttg acgccaacga
    47161 tttgatcgcc atcatataaa aagccaaccg ctttcatttt acttactaga taagccccat
    47221 cttcggcagc ctttttgata ttatcaatca ctaaacgggc atcattgttg cggaaatcta
    47281 gatagacacc cgcgcctttt agcccttctt ttttcaaaaa cggttctctt tccaatactt
    47341 cttctggtgt cagcgtataa ttctcatatt tggttcctgt cacatttgcc agtttgtcat
    47401 aaaggtccat tgctactttg accgagaaca tattgaaggt tgttgctcct tcgccctcat
    47461 agattggcaa aagcattgga tctggttttg ggatgtgtgg ggcaatactt tgtacgactg
    47521 cacgttcacc aactgtgtca gctactactt ccacatcaaa tgtcttcaga taacgaatac
    47581 cgccatgaac aagtttggtc gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga
    47641 tcaatcctgt tttgattcct gatgctgctg actggactgc cacaccggca ccagtgattc
    47701 cgccaccgat gatcaacaga tccaattctt cttgctgcat tttttgaatg ctatcttgtc
    47761 ttgtcttgtt tgaaaacata tggatttcct ccttattcaa atgcttgggt agcttttact
    47821 gcttttttcc aaccgttgta aagtttttct cggcgctctt cctccatttg aacttcaaat
    47881 agcttgccag cttcataaaa ttcgcggatc tcgtctgtat ctttccaata accgacagct
    47941 aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca agttttctgc tcgttggatc
    48001 ggtacattca agatatctgt ttggaattgc atcagatatt cattgtttgc tgcaccgcca
    48061 tctactttca atacaggaat cttgattcca gtgtcatctt gcatcgtatc gatgatatct
    48121 ctcacttgat aagcaatcga ctgtaaagta gctttaatga tatcttcttt tgtcgtacca
    48181 cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc aataaggagc acctagccca
    48241 acgaatgcag gaacaacata aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct
    48301 gaatcgctag ctttttgcaa catttgaagt ccatctctca gccattgaat cgaagaacca
    48361 gccacaaaaa tacttccttc taaagcatag taaacctttc catttatgcc gtaaccaatt
    48421 gtcgtcaata aattattttt ggataattgc ggtttttcac cggtattcat gacaataaat
    48481 gaacctgtac cataagtatt cttgaccatg cctggttcga atgccatttg tccgaataat
    48541 gcggcttgct ggtctcctgc catccctgaa attggtgctt cgcttccata aaaatgataa
    48601 ccttgcgtat aaccataaac ttctgagtta gaagtgactt ttggtaacat cactcgagga
    48661 atatttagta aatctaaaat ctcctgatcc caatctagct gatggatatt gaacaacatc
    48721 gtccgactgg cattcgagta atcagtaaca tgtgcttgtc catcggtcag tttccatact
    48781 aaccaagaat cgatcgttcc aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct
    48841 tctacatgat caagaatcca gcgtatcttc gttgctgaga aataggcatc gatcactaac
    48901 cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt ttaagttatc tgcaattcca
    48961 gctgactgtc tggattgcca aacaactgca tgatagattg gacgtcctgt atctttttcc
    49021 catattactg ttgtttcacg ctggttcgtt atgccaatcc cagcaatctg atttggtttg
    49081 atccctgatt cgatgaacgc tccggcaata actgactgta cagaattcca gatttcattt
    49141 gcatcatgtt ccacccaact ttcttgcggg aaatattgcg tgaactcttt ttgagaactg
    49201 ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg agctagttgt accttggtca
    49261 atcgccatga tatacgttga ttctgtcatt gtgattccct ccattatatg caaacgcttt
    49321 ctttaactac tcttatcata ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt
    49381 tttttctttt tttgacagtt ttttattatg aagctatttt ttaatcaatt cttttctttt
    49441 ttcagcgtct ctattcttct tttcaatcga ataatgtcgt aaggtgacgc aaattctgac
    49501 agataatatt cacactgttt tttgtttctc tggcaaaatg tgatcaccaa atcatattct
    49561 ttaccttctt cataacttcc aatctcaata ccgccgattc ccctcagttc tctgatcaga
    49621 tattgttgaa aagcaatacg ataaatcggc agactttcta aatcgattcc aatcactact
    49681 ggttttgcaa tataaaaatc gatcatcaaa agaacattgg catactggat catcaggtaa
    49741 ctaagctcac tatcttcagc tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat
    49801 ttcgccagta actgtgttaa ggaatgcccg agcattttct gctgctgttc caaaagccga
    49861 tcacgctcat agacttcgat ctttccaaca aagaaaaacc attcattgtt gatctgggaa
    49921 atttggtagc ctactgcctt ttcttctttg attttcaaac gacgaggacg ataatgccat
    49981 aacatcgttt cccgaatgta agtatctaaa acagcggttg gtaacttttt gctccgtgtc
    50041 aaatcatact gataaaatat ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa
    50101 aacatcattg tttcgtatac attcagttct gctgctgttc gactaagatg catcacgatg
    50161 attggatcga ttgcttgata aagccggtcc gactgataga gtaatttctt ttctttcaaa
    50221 gttgcgtacg tacttttttc attcaatagt ctttttttcg taatcccgag ccagcaagca
    50281 atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata cacggtccaa tcctagaatg
    50341 aacggtcttt tttctggtgt attttgcaaa aaaagcggtc gctgatcttc cgggatatat
    50401 tggaatagtt cataataaaa atatcgtatc tgtagctctt ctccgtataa ttgaccgttt
    50461 ttgatttgta atccaaattc ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca
    50521 gaagactcac tgatattaaa cgctgtcgct aatcgaacga tggatacttg ctgatgttct
    50581 aataaataga cgagcatctt aaacttcaag ctttcctgca ataaaaacaa tagaattttt
    50641 tccaaactta catcttcgtc caacacaagc agcgcctgat actcatttcg ctgtacttct
    50701 actttttttt ccatcattcg gccaatcaat tcaatatctt ccaaatattg atcaaacgag
    50761 gcttttgaca gttggacatg ctctctcaaa ccattgatag aggctgttcc cccttgcaga
    50821 acaaggtttc gcaaaatcag gatctgtaac tgctcttttc tttctaataa tgcttcaatc
    50881 ttcataggca gtattcgcca cctttgacca caccatatct ggcacacggt attctggctg
    50941 gaaaaatagc tcatgttctt ctaactgttc aaacgtccat ccttcttcta ctgcaattgt
    51001 cagttggtca agaaggtgga acatctcttt tttgctttgg agctgtgctc caagaatctt
    51061 tttttcagaa tcaaatagaa atttgagttt aactttgctt tgatttattg ctgtaagctt
    51121 gtagtctacg tatcctgttt caattttttg gggataaaaa atgctctctt cttctgtcaa
    51181 acctacgctt cctaagaaat agccaaacca ctgattgccg actggacgat aggttccctg
    51241 atcctttgtc tgtgagccac taattgtctt gctgacattt cgtgctgtac ggacagcgtt
    51301 atttactaga gaaacataca ttttttcatt tgttggacga aaagttactt ggatcgcatc
    51361 gccaatcgca taaacgtctt tttgagacgt ttgcaagtag tcatctaccc agatcgttcc
    51421 atcatcattc aatgttaatt gttcttccca catctgattg tcgggacgag aatgattagc
    51481 cagcaaaaca agatcatagt tctcctttct tttttccgtc tctacaatag cggtttcatt
    51541 ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa agcccttgat ttttcatttc
    51601 ctttactaac ggttctgtca tttcaggatc tagataacga aaaagaattg actttcggct
    51661 ttcgtaaaga tggatttgtt tttttcttga tgccaaccct tcagctattt cgattcctgc
    51721 ttgtcctgca ccaataattg caattttctt tgcattcatg atcttttgct gaagagccgt
    51781 tacgttttgt atctttttaa ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa
    51841 cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc
    51901 taaaacgact tttttttctt tcaatcctat tactgtcttt tctgtgtaaa catggattcg
    51961 atagcggtca gtcagttctt ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt
    52021 tccttttaat atcaaattga acccgctggg aatccatcct aacttcgatt gcttttcaat
    52081 caaatatatt tcacttgttg gcatcaattg gcgaagataa atagccgcat gaatccctgc
    52141 aaacgacccg ccgacaatta caatgttcat ctgcattcct ctttcttata aggtgttcta
    52201 ctgctaactt ctcaatcaga aaacacagta ttcataactc gctttcaacc ttatgataca
    52261 tagcgatgca gcagaatcaa ccctctccat cataccgtgt tctaggtctg cttctcaata
    52321 atgtactagt ggacctaatt gaagagtatc atttctgaag agatgtttca aaaaatgtac
    52381 tcttataaaa gaaaaaaact cgccttttac gtacctctgt ccaaatagga aacagaaaag
    52441 aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta gaatagacac atcatttatt
    52501 tttaattctt taatgcttca acaactgctt cgcctaattg atgagcagat tgaggatttt
    52561 gccctgtaag aagccgccca tcaatttgaa ccacttctga aaaagctgct ccagactgat
    52621 agtttgctcc tttttctttc aaagcatctt ctgctaagaa tggtacttca ttcgttgtac
    52681 catttgcttc ctcttcttca ttactgaacc cagtcactgt acgtccagct aaaatagatt
    52741 ttccagactc atctgtaact ggcaacaatc ctacaacacc gtgacaaaca gctgagacca
    52801 gcccttcatt tcgatagatt tgtgaagcga ctttcccaag atctattgct tctggaaaat
    52861 cccacattgt tccgtggcca ccagcataat aaaccgctgc ataatcattt gcatccacat
    52921 catcaggacg taaggtattt cccaaggccc gatttctgaa atcttcatct tcataatagg
    52981 cccaatcgat ttcatccata ttcaaaacac tttgcggttc taaaggtaca tatccgcctt
    53041 taggactgac aaaatctgca tcgatcccat tttttactaa tacatcataa aaatgagtca
    53101 gttcacttaa ccacaaacct gtaggacgct cgatcttttc gtacttttct acatttgtta
    53161 atacgatcaa tactttcatt ttcccatcct ctttctataa aaattgcgtt attgataacg
    53221 tgctttcaag aaattatata atgggcttgg caaataaagc tctttatatg cttcttcaaa
    53281 aggtttcgtt acaaattcat cctcacggtt ttccaataat ttctctgccc agtcaggatc
    53341 gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat ttcaatacct gctctacatc
    53401 ttcgcgattg cggatcccac cgacaccaac taaagggatc tttccttgaa tcgtgttatg
    53461 gaccacttca agaatcgatt tatcgttgta tttatcagaa cgagctattc gatcatacat
    53521 atttaatgaa acatgcagat aatctagttt tgactcacgt aatcgttcaa gaagccagat
    53581 cgtgtcttca aaacggatac ctggtgtttc atattcttct ggtgagaaac gataccctac
    53641 gatgaatggt tttttagcat attgttcaac cgctgagaaa accgttgtta ataattcttc
    53701 aataaaacga taacgatttt ccaagctacc gccccattga tcctctcgtc tattcgaatg
    53761 cggagagaaa aattgttgga tcaaatacgt atttgctcca tgcaactcga ttccatcaaa
    53821 tcctgattca atcgctctac gtgtggcttt gccaaatgct tggattgttt caacgatttc
    53881 ttcttcactc attgctcgtg gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc
    53941 agatacaggc tgttctccag ataaagttgc tcgacttgtc attcgtccac cgtggaaaat
    54001 ctgaataatc gcttttgcgc cttctccttg gattgctttc gctagctcac ttaatcgagg
    54061 aatcatttca tcatgagcga tgctgagttc acctggccag ccttttccga tatcttggac
    54121 atttgcagca ccagtaataa cagcaccaag acctttagaa cgttgtttat aataagtgat
    54181 ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat
    54241 caatcggttc ttcaattgaa ctcctgaagg taaagtaatt gattgatcaa attgtgacat
    54301 tgtcattcct cccataattc gctctgatta acctatcgag aatcatctat cattaaacca
    54361 ctcgattagt tgtacatgca actaatttgc ttaaaaaatt ttattttttt tgtaacttat
    54421 ctgttgcatc attcattgtc tgggctaatt ctttacttgc ttcatttccc aaaactgctt
    54481 tgtatgactg atgaagttct tcccagctac catcgacttc cttagcaaat tggcgtcctt
    54541 gttctgtaag ataaactaat gacaagcgac cttcgctaac tgttttgacg tatcctttat
    54601 attctaattt ttcaacaaaa cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa
    54661 tatctttttg tggtattcca tctttttctt ttaatatcaa taaaataaaa ccataagttg
    54721 gtgccagacc tgtccttcta aacgcttcat cagctaattt attcaacatg cgatctagct
    54781 ttttcacgct aaagaaaaga cattcatcta acgctttttc aattggcttt tccatttcat
    54841 cccctccatt ggcgtacata tcatagtatg atttttattt tttgtcaaaa aacacggaat
    54901 aatgtccgcc tcctctgtct agatttgaaa aaaagaagta gccatcttca aagaatttcg
    54961 gcttgttttc gacaaaactc cttctggaac accgtgaatc agaggttgtg aaaaagtcgt
    55021 ttagttttga gtatgaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    55081 tggcttaata ccgaaaaact ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc
    55141 cgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttgtcgttt
    55201 tttgagctaa tgccgatggt cagctgattg tacaccgttt attgagaggt cgtgagcctg
    55261 ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg ttttatgact
    55321 atggtgactt gtgatcgagg agttggcagg agaatactgt ttattcgtaa agaaggggct
    55381 gtgacaagac ttttgtcaca gtcccttatt tccttttatt ataggatttc gccatttgat
    55441 tggatgactt gtctgtacca atcaaatgat tttttcttta accgtttctt tgtgccattc
    55501 ccttcgtcat cttgatcaac atagatatat ccataacgtt ttgacatttg tgaagtagaa
    55561 gcgctgacga tatcaatgca gccccacgac gtataaccaa ataaatctac tccatctttg
    55621 atagcttcct tcatctgctc aatatgctgc ttcaaatagt caatgcggta atcgtcttga
    55681 acggttccat cttcttctac tttgtctata gctccgagtc cgttttcagc tatgaacagt
    55741 ggtttttgat acctatcata taattgattc aaagcaattc tcaaaccctt aggatcaatc
    55801 tgccagcccc attcacttga tggaagatac ggatttttaa ttcctccagt tattaagttt
    55861 ccttggaccc cttcccaatt ttctggatga gcactgactg cactagacat gtagtagcta
    55921 aaagaaacaa aatcaacagg gtaggctgct aagatttctt catcacctac ttctattttt
    55981 aagttgacac ctttttcttc aaacattcgt tgcgtatacg ctggataata tcctcttgct
    56041 tgaacatcac taaagaacaa agaagttctt tgctgctcaa ttgttttttg aatatcatcc
    56101 ggatcacaag aatacggaac aagaagctga tagctgatca tacaaccgac ttgtgaacct
    56161 ttgataattt catgacaagc ttttgtagct aaagcacttg ctataaactg atggtgtgca
    56221 ccttggtatt tcgcttgttc caaatgggga tggttttctt caattactcc tacgctaaca
    56281 aaagggtgat gttttacaca gttgatctca ttgaaagtca tccaatattt tactttattt
    56341 ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta
    56401 ggccatccac catattctgt aaccaaatat aacggcattt cataatggga aagagaaacg
    56461 actggctcaa tcccatattt tagacattcg tcaaacactt gatcataaaa aaataatccc
    56521 gcttcattgg gtgtttcttc atcaccatga ggaaaaattc ttgtccatgc gatagacatc
    56581 cgatagcatt taaaacccat ttcagcaaat aaacggatgt cttctttaaa gtgatgatag
    56641 aaatcatttc cgtgtctttt aggataataa tacttgctgt tgggatcctg cgccgctttt
    56701 acttgctgat gcgtcatcat atgccattga tctgtccatt tttctttagg taaatcgctg
    56761 tcgaatgtat acacatcaga cacagataat cctttttcat ctacatccca tgcaccttca
    56821 cattgattgg ccgctgtcgc tccgccccat aaaaatcctt ctggaaattt tttcatatcg
    56881 atccccctaa acttgctagt tttcttgttt gattattttt cttatgacta gtaatgatat
    56941 ttttgaatct actactattt gtaacaataa ccattgtggt cggatcatat ccctctttga
    57001 ttaattcttg caaatctact tccatcaata aatcgccttt acgtactttt tttccatcag
    57061 atactgcact ttgaaagtat ttgccattta aattcactgt atcaataccg atatggatca
    57121 gtatctctgc tccttcttca ctgactaccc caattgcatg tttcgttgga aaaactgttg
    57181 aaacttcccc gtttacagaa ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt
    57241 ctcctaaaga tccactagca aatgttggat cgtttacttc agataaatca attatttcac
    57301 ctgctgcagg agtacttaac actatatcct ttgaggattc ttcttcgatt tcatccttgt
    57361 agccgaaaag aaaagttagc atacaggaac tgatcatcgc taagaaataa ctagcaacta
    57421 gccaaacaaa tccttcacca atataaatcg ggaaagacaa aatacttctt gtagcaactg
    57481 ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa tatagctcca catatacaag
    57541 caatgtaaaa tggtcgttta tatttaagag tcacaccata aacagcaggt tcagtgattc
    57601 caaataatgc tgtgataaat gcagataaag ataaactttt tactttctta tttttcgttt
    57661 tcaaaaatac cccaagtact gctcctgctt gcgcagctac ggcagtcata catgctggac
    57721 ctagcgtatc acgaccgtag aaagaaagat tgttggtcat tactggtaca agtccccaat
    57781 gtaacccaaa aatcactaac acttgccaaa atccgccgat aacagcacca gataaaacag
    57841 ggctaaagtt ataaacagct aaatatccag ctgcaatcag ctgacttgca taatctgcaa
    57901 ttggacctac aactaataaa ataattggga ccatcacgat gatacaaatg aatggtgtca
    57961 ataaatttcg aatactttcg tgcaagcttt tgcgaatctt aggctcaata taagaaagaa
    58021 cccatacagc taaaattgct ggaataaccg tacttgtata ccttgctaga atcacgggca
    58081 tccctagaaa tcgcaaggtt atgctatcac tatatgcact gacaatcgta ggataaacga
    58141 gagctgctgc aataaccata gcgatgaaac gatctgtatt gaactttttt gcagcagtat
    58201 atgccaaaaa tataggcaag aaatagaaaa atgcatcagc tgctgcattc aaaatgatat
    58261 aagttcctga ttcagtagtt aataaattca aactcgtaca taaggcgagt actcctttga
    58321 tcaaaccact tcctgccaag acgcctaaag tcggggcaaa aacagcggcg atcatgtcta
    58381 taaaagagtt taaaatgcct tttttctcag ttcctctctt tccactagat gcatcatctt
    58441 ctagtttcat ttgtttggat aatacgtcat acacttcatt tactgcattt cctactacga
    58501 cttgaaacat tcctccagct tcgactacag tgatcacacc ttctagttgc tctaccttct
    58561 ccttatctgc cttcttatta tcttttaggg taaagcgtaa ccttgttgca caatggtaaa
    58621 cattgctgac attttcttcg cctccgacaa atcggacaat atctttcgct aattgtgaat
    58681 agtccatttt tacttccccc ttgttaatta atgccgtagc aatccttctt acaagataag
    58741 tatagttctt agaaaacggt ttctccattc aattagtacc ccttctaata gcgaacgaaa
    58801 ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc actcactgct tgaaagaaaa
    58861 caagtatatc gtttctctta tagtcaaagg agacaaaaaa aacacgcttg atcttccttt
    58921 gctgatgtat aagtggtatc tcaagctgat actctgtgat ttttgtttct acttggcttt
    58981 tgataagaca gcacaacaaa gtctcggcat ttaacggaag cataactagg tcagaactag
    59041 gagagattag ttgctgaatg gaatctatct ttttatggat ttttatttga ttttcttctt
    59101 tgtagtaatg atcaagcagt tttgttttct ttttttcttc aactatggtc agcttttcac
    59161 gcaaaatctg ataaatctcc caagattcaa actcatctag aacactaggt agataaatca
    59221 aatcatccca ctcaaaaata agctcttgct cagtagtcaa aattgcatca tattccgtta
    59281 ttttgtttga ttgatttttg aaaagataca ctggttctat ccggcattct ttgatccaca
    59341 tgttcatact ttgttgaatc tgttttttta gtgtattcaa aatactaatt ggctggttac
    59401 taacaatcag tatctcccct tgttttcgaa aaggattcaa cgcttcggca agatacaaaa
    59461 caaaataagc gctttcagct aaattgggac gtaattgaaa atgtattggg aagaaacgcc
    59521 gaattaaata aatttctaaa ggataacggg agatgatctt ttgtacaaaa tgattcatgg
    59581 tattatgtcc ggcatccatc ctcgttttca tttgtttgat atgggttagc aaaaattctg
    59641 tttcatggaa caataacgaa acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg
    59701 ctcttagatt cttctctatt tctttgtttt tttcatcatt aatcatcaaa taatttaact
    59761 ctaatagtcg attttcaatt gcttgttttt cttgattgga aaaagaatag ggtagttctg
    59821 atgataactt ttttgtgagg gtctctacca tcggaataaa taatgtatac ctgtttgttt
    59881 tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga
    59941 agtcctctcc tgaaacaata taattatatg atataagaac ctcttttaat gtcttttgga
    60001 cagtcggcat gatattggaa aaaattgtac tgatttgttg aggaaacctt gcgtgttgga
    60061 tcaccgtttc tgttccaaca agagataaga aaattctttt tgaattttct tcagcaatga
    60121 tttttagtcc ttttgtcgac gaaacttcca gttctaattt aggattgcgc tcgatccaac
    60181 gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga aagaaagaaa atgtctgcta
    60241 tcttttgcat actgacatac cctttttgaa acaataagta cgtcacaata gttgaagctc
    60301 ggaaatccat gctggaatag attttatgtt tcgtcacctc ttgtatgatt tgctgtttag
    60361 tttcagattc aattgattct aaaaaataac ctgaacgttt atttgactgg ataatgattt
    60421 tcttctcgta gatagcaagc atatttttga tttcagtttg gatcgttttc ttgttggtac
    60481 ctaaagcttc agctaattct tcgcttgtta tccaatgatt cttatttctt aatactacaa
    60541 gaatttggag ttgtcgttta tttaagtcca tttgcttaca cctcctcttt gttataaaca
    60601 tacaggaaaa acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag
    60661 aaagatctat attcaaggtt tttaccatta gactggataa tttgtttata atattcaaaa
    60721 ctatcttttt tcaatctcct aaagcttccg ctcccatcat tttgtctatc cacataaata
    60781 aagccatatc gcttcctcat ttcccctgtt cctgccgata cgatatcgat tggtccccac
    60841 cataagtaac caatgatttg gcaaccatct tttattgcct cagcaatttc ctgaagatgt
    60901 atttttagat actcacaacg ttttggatcg tggattcgat tcaattcatc tggtgcttca
    60961 tctagtccaa tgccattttc aactatgaat aatggtaatt cataacggtc tgtcaattca
    61021 ttcatgacat aacgaaaccc tagaggatca attggccaac tccaaggttc gggagatttt
    61081 tccttcaaaa agggattatc gaaaccattt gctccgccag tatctacgcg catttctgct
    61141 tcttttttat agacagcgct acgataataa ctaaatgcaa tataatctgc cgtgttagct
    61201 tgcaagcact gttcgtctcc atctaacatt actggtgcac agtcttcttc cttccatgtc
    61261 ctgtaaatat aagctggata atgccctcgg cacatgacat cggagaaaaa ccatgaattg
    61321 cgtttaaatt gcagtgaacc aaatacattc tctggatcac agtcatatgc ataagtcgct
    61381 aatgcagaaa agctacacat aatacccatc ttagcatttg gatcgatttt ctttaataat
    61441 tgagtagcac gggcgttcgc tagaaacata tgatgacagg cttgataaat cacttttttt
    61501 gttagtgtag caatgggttc aagtttatct ctagcaggga atgtaaccaa tccagcagct
    61561 gcataaggca tacgaaaagc attattgatc tcattaaagg tcatccaata tttcactttc
    61621 ccttggtagc gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat taattttcga
    61681 ttttgccacc ccccatactc tcctaccaat gctaaggggg tttcataatg actaagagta
    61741 attactggct cgatatcata tcgatttaat gtattgatta gtctgtcata aaatacgaga
    61801 ccagcctcat ttggcttctg ttcgtctcct cttggaaata ttcgtgccca tgagatgctt
    61861 gttcgaaaag cttttaaacc taactccttc agtaaacgaa tatcctcttc aaatcgatga
    61921 taaaaatcaa tcgcttcatg agaaagataa tggagtgatt cgtctaaagc aatctcccaa
    61981 atcttttttt cttcattcca ctgaatacta ggatgctggt tcatgatccc gataagcgta
    62041 tctgtcacat taggtaattt gccgtcttcc aaccatgcac cttcacattg attagcagct
    62101 actgctccgc cccataaaaa attttctgga aatatttcct gatccataac tatctctcct
    62161 cgctatcaac aatttcttac ttgaagtata gctcgattca tcaaaacaag atattctaac
    62221 tatttcggca gaagagaaga aaaaaattga atgctactct tcatttttaa tatttatata
    62281 taacaaatac ataagacgat ccccgaaaga aaacactcgt taaccatcta agcaaacgat
    62341 ttattttact tccaacacaa atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa
    62401 ctgtttcaga ggataatttg acacaataac ccttaaagct tcacgtggaa tagtgaacgt
    62461 ctgcttttca ctggacatat tacaaataac gagccattgc tggtcttcat atatgcgaga
    62521 ataggcaaag acatgctcag gcgtcttttc cactaattca tagtctcccc agacgatgat
    62581 gtcatgtttt ctacgcaaat cgatcaattt cttgtacata taaaaaacag aatcttgatc
    62641 tgctaacgct tcttctgcat tgatttcttt ataatttgga ttgactcgaa gccatggaat
    62701 tccagtagta aacccagcat gttcacgggc gttccattgc ataggcgtcc tagcattgtc
    62761 tcttcctttc gccttgattg attccaagat ttcctctttt gtaaatccag aagaaatcct
    62821 ttcgttatac atattgattg tctcaatatc ttttgcttcc gcgatatcat cgattgggca
    62881 attcgtcatg cctaactctt ctccttgata gatataaggg gtgcctttca tcatatggag
    62941 caagatagca aacatcttgg cactttcttt tcgatacaca tcgtcatttc cccaacgaga
    63001 aacaattcgc ggaagatcgt gattattcca aaataaacta ttccatcctt catcacctaa
    63061 agatgtttgc catttggata atactttttt cagttcagag atactcaagg gtttcaaatc
    63121 ccatttgtct ttgccttctt gctgatctaa tcctatgtgt tcgaattgga agaccatgga
    63181 taattcattt cttttaggat tggaataaag tttagcgatt tcggtagttg ctccccatgt
    63241 ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct tgattcattt cctgaagata
    63301 ctcatgcagt ttaggtccat tacttgtgat ttctttatcc ggttctttgc caatcatatc
    63361 gatcacgtcc atacgaaagc ccccaatacc tttatcgatc cagaaattca tcatttgata
    63421 aatctcttgg cgaacctgtt tatttttcca attcaaatca ggttgacgct tgctatacaa
    63481 gtgcagaaaa tattgccccg ttttttcatc caactgccaa gcagaaccac tgaatattga
    63541 gcgcaatcta ttaggctctt ttccattgac cggatcacgc caaatataat agtcccgata
    63601 aggattttct ttatttttac gggcctcaat gaaccacgga tgttcatcag aagtatgatt
    63661 caccaccaga tccatgatga tacggatatt tcttttcttt gcttcttcaa tcagttcttc
    63721 catttcttcc atcgtaccga attctgccat gattgcttga taattactga tatcataccc
    63781 attgtcatca ttcggagact cgtatactgg agaaagccaa attgcatcaa tacctaaata
    63841 cgccagataa tccaacctgg aaataatccc ccgcaaatct ccaataccat cttgatttga
    63901 atcttgaaaa cttcttggat agatttgata aacaactgaa tggtgccacc atttttgttc
    63961 catgattttc ctcctataaa tggatgtttc tattttttgc ttaatcattt attttagtct
    64021 ctctcactaa aaattgtaac gctttcaaat agctattgca ccttgttatc ggtaacagaa
    64081 taaaacaaaa aaacagctga ggaaattccc tcaactgttt tttgaaagga ttgatttatg
    64141 cagggaagaa tgctgatttc acaacttctt tataaacggt gttcactcag ctgtcccttg
    64201 gtattagctc aaaaaacggc aaaatataag aaactatttt ttcgtttttt tctctaatac
    64261 tcaggccaga acgctgattt cacaacctct ttatttcaca ttcaatatcc acatgaatcc
    64321 cacaaataca aagaacagac catacatgat tggagatact tcttttcctc ttttagctgc
    64381 taacatggta attggataga agataaaacc taatgcaatc ccatctgaaa tactataagt
    64441 caaaggcatg ccgatcaaga tcaagaatga agggatcgca atctctaagt ttccccattc
    64501 gatttgtcgc aaagattgtg ccatcaatac tcctaccata atcaatgctg gtgcagttac
    64561 ttgtgaagta acaactgata gtaatggaga aaagaacaag cctaacaaga agaaaatccc
    64621 tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg ataccagctg atgattctac
    64681 aaatgcacca acaggagaag taccaaataa ggaacccgca agcatagctg ttgaatcaga
    64741 agctaaagct ttccctacac gaggcatctt gttgtctttc atgaaacctg cttggtttgc
    64801 taatccaact aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac
    64861 gacccacatt tgaagagaat tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg
    64921 tttcaaactt ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac ctgtgaccag
    64981 acctaaaatc gttgtagctg ccatcccgat gaagatgcca cctggtacac gccgaacaag
    65041 caagatagca gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga
    65101 tcctaatcct actaatgtag attcattcga aacgatgatc ccaccttcgc tcagaccaag
    65161 gaaagcgata aatagaccga taccgccaga aatcgcatat ttcaaatctg ctgggatcgc
    65221 atcaatgatc atttcccgca gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac
    65281 gaatacgccg gctaatgctg tttcccatgg aatccccatt cctaaacaaa cagaataagc
    65341 aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt ggataacggg ctaatacccc
    65401 cattaaaaca cagccgatcg cactggctaa tgcagtcgca gtaaacactg ctccttcatc
    65461 cattccagaa gcacctaata cagttggatt tacaaataag atataagcca ttgagataaa
    65521 tgttgtaaat cctgccagta cctcacgttt taccgtggtg ttcaattttt ctatttcgaa
    65581 gtaggaaagt attttttcct tcatcttcca tccccctaaa tccgaacatt ataaataaag
    65641 tccgttaaaa tttcgattta ttaataaaac aacgtgtttt atcatatact aaaacaaaac
    65701 ataagtaaac aaaaaattcg gattttaagc aaaatccgaa cgttccatct gattaaatcc
    65761 ctttatctca aagttcatga acattcactt tggattacaa gaatttcttt attgtgttaa
    65821 taaagtttct tcattctagt ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt
    65881 tctgttcttc tttgatgaat tcttttgtat cgacaggatt cagattattt agtccgccaa
    65941 aacctgtatc gaccattttt ccccagcctt ttgtcttctt ttttcctgat ttgagattct
    66001 ttgctcgaag cttctcacta tgttttgctc gccgatactt caaataacct tctaatcttc
    66061 ccattagatc cactgttttt cctccttttt attcaatcag acgactcagc ttttcctata
    66121 acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg
    66181 atttctcctg gttcttccat gttctcgtag atagtttcaa attcatccgt aaaaaatact
    66241 aaagaaggcg gtggtcctaa tacctctggt gaaaacttct gcacaaatac ttttgggaat
    66301 acgcctaatt ctaattcttt tgagatgctg agtacaatac tcttaaattc ttctggcaat
    66361 tcgattgttt cagaaatttc tgcaccaatt ttttctacaa agaatcggca aatcatctct
    66421 acgtcattga cataaagcat cacacgtgtt ttttgtatca tcatttgacc cttctttcta
    66481 tttgattttc tttcctgtct ataaactgat tccattatat cacgaaagcg caaacacctt
    66541 gtgtttgata ctattcaaga aaaataaagc aatagtttct aggattgctt tcctccacaa
    66601 acttttttta caaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
    66661 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
    66721 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
    66781 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat cgttctttgg
    66841 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
    66901 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
    66961 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
    67021 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
    67081 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
    67141 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
    67201 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
    67261 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
    67321 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
    67381 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
    67441 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
    67501 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
    67561 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
    67621 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
    67681 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
    67741 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
    67801 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
    67861 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
    67921 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
    67981 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
    68041 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
    68101 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
    68161 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
    68221 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
    68281 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
    68341 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
    68401 ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
    68461 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
    68521 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
    68581 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
    68641 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
    68701 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
    68761 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
    68821 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
    68881 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
    68941 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
    69001 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
    69061 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
    69121 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
    69181 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
    69241 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
    69301 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
    69361 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
    69421 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
    69481 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
    69541 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
    69601 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
    69661 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
    69721 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
    69781 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgccataa cacttcaaag
    69841 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
    69901 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
    69961 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
    70021 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
    70081 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
    70141 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
    70201 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
    70261 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
    70321 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
    70381 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
    70441 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
    70501 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
    70561 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
    70621 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
    70681 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
    70741 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
    70801 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
    70861 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
    70921 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
    70981 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
    71041 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
    71101 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
    71161 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
    71221 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
    71281 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
    71341 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
    71401 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
    71461 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
    71521 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
    71581 cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
    71641 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
    71701 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
    71761 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
    71821 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
    71881 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
    71941 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
    72001 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
    72061 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
    72121 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
    72181 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
    72241 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
    72301 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
    72361 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
    72421 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
    72481 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
    72541 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
    72601 aagttaaact tccaacgatg atgctcgaac cagcactcaa cacttgaaat cctaggagtg
    72661 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
    72721 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
    72781 taacgcccat gtttttaccc tcttttcttt ttgtatttag ataactccct tatcttgcag
    72841 aactggggtg attttttctt caatttcatt ttttgaaaca atatttttca aatagatcat
    72901 cgccgtttta ggatcaactg cgaacttttc gaactgcgaa cgaaaatttt ctgctgtgat
    72961 gatcacatct ggtttcatgg aaactgcgga agaaatatca caatggtcca gtttggcttc
    73021 tactcctaat ttgttcaaaa catcctcagc agacatctgt gctgcaaaac tacttcctaa
    73081 acctgcaccg catacgaata aaatgttcaa tttgttcatc tttgttctcc cccgtatcat
    73141 ttattatttc tcaactactg aaaaaagcag ttgtttgaaa tcttcgatat ttgtgcattc
    73201 aatcaatcga tccaacttct cttcgttgtt gatcaattca attagttctt tcatgatgtt
    73261 cagatgcgag taggagtcta ctgctgccaa acaaaaaata atctttactg gatccatttc
    73321 aggattaccg aaatcgattg gttgctcaat cgtagctacg ctgactccta atttattgac
    73381 cccatcttct ggtcgggcat gggctagtgc catgtgtttg ccaatcacga tatacggtcc
    73441 gtaatcattc actgcacgaa tcatcgcttc gacatagcgc tcttcgacaa tgttttcttt
    73501 aaccaatggt ctagcagcta gttcaatcga ttcctcccaa gtagtggctt tttctttgat
    73561 catgatattg ctgtccgtca aaatatcttt taacataggc tggatctccc ttttgttgat
    73621 ttctaaattg tttgtttcga ataatttttc taacttgcca tagatgattc gatctactcg
    73681 gccaccactt tctttcatga catctattat ttgagtgaat aactccgtcg attcatgact
    73741 gttaggctct aaccgctgta gttcttcatt tcttttcaaa aagtcttcta taaccaagat
    73801 attcgtctca gttaaaatcg gatcgacaac taaaagcggc ttatttcgat aattcaattg
    73861 aaaggtgctg aaaaccagat tgacatccaa tttatctact aattcgattt cttttgaact
    73921 taaaactgca gtaatctcga tctgaggaaa cttttctttc agattctctg ctaacagatt
    73981 tccagtagcc atcccatggt tacagacaac gactgattta taaacataat tcaagtctcg
    74041 cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga aaagctatct cgtcctccag
    74101 tatcttgcca ccaattactt cttcgatcgt aggaacaaaa tgagtgactg cgcggtagat
    74161 tgggaaataa tttgttcgga tgttttcttt caatggattc atgacttgga tatgattctt
    74221 gattcttaca atcagtgcag ccatgtgctt atagagattt tcgaataaag cttcttcctt
    74281 tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg atcaattgga tcgttagtaa
    74341 ttgagcttgt acccactcga ttgaattgga gatatctcta gcattgaacg tctcaatagt
    74401 gaaacgaaga taatttcttt ctacttgcgg gacattgtcc aattgaaatt cctcgatcat
    74461 gtggtcaata aatgcagaaa agctactgtc ttttatttct gtatttttcc aattaatggc
    74521 agagataaat tcatgtttcc tgattcgctg gatccagatc aatgtaaaaa gcaggatttg
    74581 ttttttataa atatcatctt cttgtttcaa caacttattg gaatagatct cctgtatttt
    74641 tttgatttcc accaaaggaa aataatggag aaagattttt tgagccggtg ttaattgtgc
    74701 atcttgattc ataaagaagt cggtcattcc cagattctga ttgataaaat catagatcat
    74761 cgtacggata gatcgttctg aacctttata aaccagtcct tgctttccat aactgacgat
    74821 ctctatccca taactgacca gtgttgctcg taaccgtctc atatcttcat ctaatgtact
    74881 tttagagatt tgaaattctt cttctttttt gtttagatgg atcgggattc ggctgtaagc
    74941 aacggctaaa actagatcaa aaatacgttc gcgccgattc aaaaagccgt ctttcacatc
    75001 ttttatttca gtttctaatc gagcttgctt ttccttttcc agcttcaaca aaaaaccttt
    75061 gttgcgaatt gtacggatct ccggaagctt tttactgctc aaccagatat tcgcttcaag
    75121 aacttcctga cggatcgtcc gctgactgac attgaactca cttgctagat gttttgcttt
    75181 tactggatat tgggctgtca tgattctgtg aagtatttca attgttcttg ttttcataga
    75241 aatgatctcc ttctgatttc aagtatatgg ttccgcttac ataataaaaa gtagatattt
    75301 ctgccacttg aagtggaaag aaagtaagca aagcaaacaa aaaataagcc atatgctttt
    75361 tcatctcctc aacgaaaaag acatacgcct attattacaa aaattttgcc catctataca
    75421 aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacaacta aactaaaata
    75481 gtgcttccta tttttcgttt ggttgttctt attatcgcag atgcaagctt ttaaacacca
    75541 tttagtggag aggttgtgac agagatgttc agccttgagc gataagaagc gattttgaaa
    75601 aatcgcttcc catattttac aaaatcgtgc cttattgccg ataggctatc tctggcacac
    75661 cgtttattca gaaaaaggag ctgcgacaag gcttttgtca caactccgtt cataaatatt
    75721 tcctgtcaat ccagcataat cggctgaata aaatcggctt gccatctttt ttctttctca
    75781 gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt gtgtcttact tcctgagatc
    75841 aatcgcccat cttttcgatc gactgaataa tcacagcatc tgaaatcaat tcggatggca
    75901 aaactatttt cgtggatctg tcgataattt cctacttcct gaatagttat ttttttagta
    75961 tgatttctta caccgtctgc tttgtttttt tgcagaactt tctgatgttc ggcaaacagc
    76021 ttgtctgtat aacagcttct ggctttttca ggagtttctt cttcccaagc ggattggatc
    76081 ttcatgaata tcttttggat ttccagaaac tgtgctttct tttctttagc cgttcctgac
    76141 atgcgggaca atatctccgc tttcagcctt ttcttttgtc ttttattctt gacgatagaa
    76201 aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa gaaatcccgt ttgctcataa
    76261 ctagaaggac ctgtacctgg accataatac ccataagaat ctctgccgta agaacgactg
    76321 ccaaaataac tgcccccata tgaacgacca ccaaatgaac ctctgcccga acttcgaccg
    76381 ctagatgtac ctccaccatg accacccgcg acagcgaatc cttgggtcgg cagagcaaac
    76441 aaacataggc ccactaacaa aatgattgct aaataattga tttttttcat tgtcttcctc
    76501 gttatctttt attacaaaaa actattttta tgatagcctt gctaaaagga agagtcaata
    76561 accagatgca taaactgttt ttttagcaat tttgtcttat ttagtcattg agtaacatcg
    76621 gacaaatgta tgtgtcggca gtccataatt cctctatctt cagcatgaac aagcttgcag
    76681 acaagagatc tgcgcttcct ccaggagaga tttggttttc ctgacaaaag tgcaacagtg
    76741 ctgtaaatgg acgatttggt aaagcacttt gactagcagc atgagcaagt ttttgcattt
    76801 ccactaacgt tttctgatca ctgcgatgca atacgcatgt gtcatctagc gaactcatca
    76861 aaactaacaa catatgcatt tgtttctgta catatgaatc attatctagc tccttagctg
    76921 cttgaattgc aagcctgata tgcggatacc ctaatatcgc ttcttcatat gcaccattta
    76981 cgccatattt ttgcttcacc ctatgaccat gagtggtttt tttatgacga tattttgtat
    77041 caggaaaagc agcaagcttg ctgacatcat caagtatttt cgataaagaa aaaggctgtt
    77101 ccatactgat tttttgtgca atgacactga tcaatagccc cattgcccaa atcgctcctt
    77161 tatgggtatt gatgccattt gtagcaatca gcattgcctt ttccgctttt cgcccaatct
    77221 cagcaattct ttctcttaac tcctgatcaa tagcatgatg ccatgctatt tgagccattt
    77281 cttcaaaaaa aggagttagc gtcttagcag acttaagaaa taaagcatag tccatatcgg
    77341 aatgtgcacc cgcatccagt gcatccacta atccaggttt tggacttaat gttacttctg
    77401 cgatcaatgc ttcctctacc aacgcagcga gttgctgagt cactgctttt tttcgttcgt
    77461 catttatcat gacactcact ccttcttcct actattactt gctgaaacct tttttgatag
    77521 ctacccaaat gatatgaagt aaccagctta aagcaaatgc acaacttgcc gtcattagat
    77581 aagctacaag aatcatgact cctgaaggta actgcagcca actatcttta aacaataata
    77641 acagtacttg gaaaacaaat acattggcta aatacgtacg ataagcataa acagacaacc
    77701 atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat tttggaagcg cctatgaaga
    77761 cagcaaagat gatgactaaa ctgtataaac tttggatcgt attcaagtaa ggtgcattcg
    77821 caaagctcaa gtctcctgga tgtgcgagca atgaacggac agaaaagaac gcgataacta
    77881 aggcaacagg taagaaaaga taacgtactc tgtccaaata ttgatagact ttttcgtgat
    77941 aaacaaatgc ggctacccca taaatcatga aactagcaac aaaccgatct aataagtacc
    78001 agctgtcatg ataaggccca gtcaatactt gtgtttgata gaacagatac catgcgacat
    78061 ataaaatcgt tgaaccaatc aatactggcc atacatattt tttattagca aatactttgt
    78121 aaccgagcca aacaaaaaac ggcatgaaca attggatctg cagcatcata acggtatacc
    78181 ataaatgagg cgctccatca cctgtgacat atttcagtaa aaagcttgca gtatccgtat
    78241 acggagtttg ttgttggact gctggaaata gccaaagata agccgtcgtc catagaagat
    78301 acggaacgac tagttcaaaa aacttttctt tcaaaaattc ccaataagac aaatgctgac
    78361 ttgtttttac catattataa acaattgcaa aaataaacat tggtgctgtg tatttaacga
    78421 tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct gtggctgttt gccaaagcaa
    78481 agctcaatat cgtttgcatc ataacagccg ttacagctga tactttctgg aagtcactta
    78541 cgttgatttt gatttttttg ttttcaaaca tctctttctc tctctttttc tttattacca
    78601 aagttctttg gttaatttag gtccttcgac tgttttcaat agaatttttt tctctggctc
    78661 tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc actacttggg tatcgaattg
    78721 ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc aattcaatcg gtgaatttgc
    78781 gtacagcagc aaatcaaaat cactggtttc ttttactgcc ggaatcccag tcgataattc
    78841 aaatcctaag ctgccgccga ttccccattt acatcctgat aatatgtttc tagcagcatg
    78901 aaattgctga taaacaggat aatccatgat tttctcttgt ctatacgatt cttgttcgat
    78961 gatgtcccac ggatgaatga cttgtttgac ggcatgtttt gagatttctg ttgaccatct
    79021 ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt tctgcgtgat ccgtcgacgc
    79081 acgacgaact gttccgaaca tcttttcagt tagaacgacc cattcaggca aggtgaaccc
    79141 ttctatttca tgtttatcaa aaaggacaat atcatgtgct tgaatcattt tttatgcatc
    79201 ccattcttcg tccattttgg cacggacttt atttgtttcg acacgtccga atgttttggc
    79261 tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt gtattgcgga catcttgaat
    79321 ggcttcattg attgcttcga tgactttgct gacattttgt tctgaagcag cttggtcggt
    79381 gatatcttct aggaaatgat agatcgctcc tagttttgta taattattga tatcgtaagc
    79441 gatagctgga actttttcag cagctgcttc gatttcagca accgaacgtt ttgtcacacg
    79501 tgcagcactt tctttagaca tcgcttggac ggtaatttct ttattgttta aagcaatcaa
    79561 gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa atagcatttc ctgcaatcaa
    79621 tccaataact ggatgtccag cttgacggag ttttgcataa gcagcagcac tattagccaa
    79681 agccacatgg ataccgatca attcttcgtt atagccgtat gcttggcttg gtacatcgat
    79741 cacaacgatg attggccgtt tcactttttt gtctttatct tcttcgtaaa cgtgatccaa
    79801 aatttgtccc atgcggaaac cttcaactaa accaacttca ccgtttcgta cacggtacat
    79861 cgggttttta ggatcaggta cgattgatgt cacgatggct tcttctatga atttacttga
    79921 tttaccataa tacacagtac taatagaaga agttggattt ttcatgccag ttagtgccgc
    79981 aaaccaatcg taaccaacac ttgctgcagc tggttcattt ccttctgcta cttctggtaa
    80041 gtcaattgat tttgcttgat ggttagcaat tgcttcattg taatcgtcaa tggccattgg
    80101 ttttgaaaga tctaaagaat caagtaatga taaataaaat gctgagcttt cactgcgatg
    80161 cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct ttgattgctt ctacactatc
    80221 agtcactaat tcatcaatga tttttgattc tacccgttga cgagatccta atgtattcca
    80281 aatcaagtct ttgtcactag aatcaaattc tcgaacgcct gcttcttgtt caataacttc
    80341 tggtccattc aaaccgactc tcgctttttt tgttgcgatt aaataactca ttaatgctga
    80401 agtaatcgac ataccgccaa atgatcctac acgtcctgga atcaaaccga tgactggtac
    80461 atattgtttt agagcaatga ttacatttcc gatttctgaa atagataaca gtccgtagtt
    80521 tgcttcttgc aaacgaacac cacctgtatc tagtacaatg actggaaaga tctcatttcc
    80581 ttttttattt tcttctaaca catgttcaag tgcagcaacg atttttgcac cggaaacttc
    80641 accgataccg ccaccttgga attttccttc catagcgatt actactgctt gtttgccatt
    80701 gatcgtgcca cggctgacaa cgatcccgtc atcactttca gggacgattc cttgtgctgg
    80761 taaacttggc gcgatgattt gatcgaatgg atcaatcaat tcacgtccat tgtcaagtag
    80821 agcgttcact cgttctctgg cgtttaattc tacaaaacta tttttcatct gaaagttcct
    80881 ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt agcaccgaag tcattgatca
    80941 cgatatctgc tgaaattgga taacgatcaa aaaatctcgt taatacattg ttccatactt
    81001 cgccgaagcc atcgcttcct gtacaaattt ttacagtaga ttctgttttt tctgttggaa
    81061 acatcaagat ttccaaatct cctgaaccga ccactcctac atggattttc ttattgatcg
    81121 gctgtttcgt tggatattta taagctaatt gttccatgga tctctcccct ttttgtaaaa
    81181 cagagcatac acgttctgct tattcgtctt tcttcttcca tctgggtaaa tggcgacaaa
    81241 aggtttttct cgccaatttt atgaccaagt tctgaatttc attggtggtt catacaggcc
    81301 atctgaccat tcgatcaaat catcgatggt ttgtgcagct aataatgaac gtttcgcttc
    81361 actacgtttg atgcctagat cttctggtaa tttcacgatg ccggctgcac gcaagctatc
    81421 tagtgattct ttcgtgctag tcattccaag aggtgtaaca ccgccaattg ctgcaattgc
    81481 tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa gcaattcctt cttctgtcac
    81541 gatatgtgta gtatcttcgc tataaatcat cactggtgca ttggctaagc ctgcttgttt
    81601 ttgaacagct agtgcatcta attcttcgac aaagactggt tttttatttg aaccatatgt
    81661 ttcaaccatt tgaacaacta atttttggcc tttccctaat ggatcatcat tgtcgcgaag
    81721 tgattgccat gctggtgtag aatgacgacg accgccagga ttatgtccca tgttaggcgc
    81781 accgccaaaa ccagataaac gtccttttgt tacggtagaa gagtttcctt cgtaatctaa
    81841 ttgcaaggtt gaaccgataa acatatcgat ggcatattgt cctgcagctt gtgataatgt
    81901 tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag acatctgggc gagcagcaat
    81961 atatttttcc ataccaactt cgccaccaaa actatggatg ctttcaaccc aacctgattc
    82021 gatggctgga atcatcgttg gatgtgggtt caatacccag ttcttcgcga ttttcccttt
    82081 taaccctagt gattcaccat aagttggcag taacaattcg atagctgctg tattgaatcc
    82141 aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag attcctttga ttgccatcat
    82201 tgccattaga atttgcaatt ctgtgatgtt ttgaggatct cgtgtgaata aagcttctaa
    82261 ttgataaggt ttatctgctt tcacgacaac gtctacccag tcaccaggga tatctacacg
    82321 tggtaaagaa tcgacaactt cgttaacttg aacgatcacg ataccatctt tgaaagctgc
    82381 agcttcgatg attgttggtg tttcttccgt attgaaacct gtgtacaagt tgccgtcttt
    82441 atctgcttta tctgcagcta ctaaaacaac atttggaatc aaatcgatga ataagcgtcc
    82501 ataaagttcc aaataagtat ggatatcacc taatttcatt gtgccgtctt caatcatttg
    82561 ggcgacacgc aagctttgtg caccagcata agagaaatcg atcttgcttg cgattccttt
    82621 ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac atgatcaaat gcagatcatt
    82681 cactttttct gcatctactt tttcaagact ttctgaaaga aaacttgctt gtttttgatt
    82741 gtcgccttct aatacaactt tgtcgccggg agcaatcagt ttttccatta cttctacgat
    82801 tttttcagtt tctacatact tgccattgag caagttgctg attttttcca atcgctgcgc
    82861 ttttttttcg cgtttagaag cccagttgcg gtttggtttt tccataatta gtcatctcca
    82921 tttttgatat agaaacgtgg cgtcatcaat accatgttgg tctagattga tcgtacggat
    82981 ccctttatct acaccaaatt ttgtatgaat cagctttgtc aaagtattgc cggggggaaa
    83041 ttcaatcgtc agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac
    83101 agggaaagct aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct
    83161 tccatccgta ttttttatat acaaacatct agcatcagat agatggacag actgcatata
    83221 cggcttgaat ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt
    83281 cagcagcttc gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc
    83341 ttcgatactc ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg
    83401 atggtcagga tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac
    83461 tactacgccc attgcataac cgcttggata agcttctttc attaagactg cgcggtgata
    83521 gactagttcc gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc
    83581 accaatcgaa tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa
    83641 ataaaatact gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc
    83701 ttctttatca actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg
    83761 gatatccgct aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact
    83821 catgagcctc gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta
    83881 taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa
    83941 ttatgaaatt gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc
    84001 aagcagccaa agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg
    84061 aaaacgaatt aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt
    84121 atgcagaaga attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca
    84181 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    84241 cctctgtttc tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat
    84301 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    84361 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    84421 ttcctaagaa acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca
    84481 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    84541 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    84601 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    84661 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    84721 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    84781 aattttattt aggaaaataa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt
    84841 ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa
    84901 tttgagaagc aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca
    84961 acctcttctc tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat
    85021 ctgctagttc atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat
    85081 tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat
    85141 cgaccttcaa atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt
    85201 cttctggaac agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc
    85261 aataatcagg aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt
    85321 gatcgattag ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt
    85381 attcaaagat accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt
    85441 cgatattttt cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac
    85501 catctactat taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc
    85561 atttttcaag ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat
    85621 gattgaaaaa tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa
    85681 gggagtctga ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg
    85741 tcatgtttct ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc
    85801 tgaaatcata gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca
    85861 gctgcccgaa ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca
    85921 aaatttatta acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact
    85981 gctgaaaaat ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg
    86041 gctttcttat gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta
    86101 cgacttgcag atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat
    86161 tcgtactttt tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga
    86221 ttattcgatc catgaatccc attattttca atttttaaga aacctcagtc aaactgaact
    86281 agcggttgaa acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat
    86341 aaaagcgaat tgccgattag attcttctat ccataccaac agcacactct cctccctgta
    86401 tgactctgca ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct
    86461 ttcttttgct tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga
    86521 aaccgctcgc tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga
    86581 actagcttca gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc
    86641 tctcctcata ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
    86701 ccatcgtttt ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa
    86761 tttactgaac gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact
    86821 cttgtctcta ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta
    86881 ttttattttc caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta
    86941 cttaggaaaa cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt
    87001 tgaaaaaaca gatatcttga tttccaattt ccctctagat tctctagata tccctattgt
    87061 atacatttct tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa
    87121 aactttttta tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa
    87181 acagtttgtt catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg
    87241 ctccaatcaa taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga
    87301 ctggtagaaa aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg
    87361 ctttgttttg caaaagacct tggttcaatg acggcttgga caactctctg caattgatca
    87421 tgtaaacagc ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc
    87481 cttcaaactg catagccaat aaataggcag gaaccatgat cagtagggag acatagatga
    87541 tccgaaacaa atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca
    87601 tgatttctgg atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca
    87661 tattgaccca taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc
    87721 aaatcaagat caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca
    87781 aattatccaa aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat
    87841 ctcctaaaac catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa
    87901 ttcctacatc ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga
    87961 cgatttctcc atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc
    88021 gagcaaagac atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca
    88081 aatcgatccc ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt
    88141 ttgctgtgtc tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt
    88201 cctttacagc tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa
    88261 aagttaattg ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag
    88321 caaatcctaa aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt
    88381 cttgttctgc agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt
    88441 aaatcaccgc tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga
    88501 acggaatctt tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca
    88561 gcgcaacttc tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta
    88621 attcctgaca gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca
    88681 caactgtcat ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag
    88741 aacctaatgt ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat
    88801 gtactccaac ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca
    88861 ccaccaaagc aatcgtagcc gaaagaagaa tatgccaatc cataccatat cgaaatgtcg
    88921 taaaaataac taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc
    88981 ggttgatttt tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga
    89041 ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt
    89101 gtacaagcgt tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca
    89161 aaacggcatg acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta
    89221 ataaatcatg tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga
    89281 caatgacatc tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata
    89341 gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc
    89401 tttcttgcca gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt
    89461 caatatattc actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca
    89521 gatcagtaaa atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt
    89581 cattatatcc ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg
    89641 atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc
    89701 ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat
    89761 ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct
    89821 attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg
    89881 aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa
    89941 caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac
    90001 atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa
    90061 aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa
    90121 taacattcct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa
    90181 ccaatggggc aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac
    90241 tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc
    90301 gccttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca
    90361 atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg
    90421 ccaaggcaga cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac
    90481 ggttaagccg atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac
    90541 cattccagca aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc
    90601 gatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa
    90661 aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc
    90721 aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac
    90781 atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc
    90841 aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata
    90901 gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc
    90961 ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc
    91021 tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa
    91081 tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa
    91141 gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta
    91201 ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta
    91261 gattgatcca tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata
    91321 aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa
    91381 atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga
    91441 catagacgat acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga
    91501 cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa
    91561 taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg
    91621 caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt
    91681 ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac
    91741 caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt
    91801 ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta
    91861 acgcggcaaa tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca
    91921 cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg
    91981 atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg
    92041 gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca
    92101 cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat
    92161 tcaaaaaagt gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca
    92221 aaccaagcgg cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca
    92281 tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt
    92341 ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa
    92401 attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa
    92461 aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg
    92521 cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca
    92581 tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga
    92641 acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt
    92701 gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt
    92761 ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt
    92821 tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat
    92881 cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta
    92941 taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga
    93001 gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga
    93061 atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca
    93121 ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg
    93181 atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca
    93241 ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg
    93301 agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg
    93361 gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct
    93421 tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac
    93481 acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct
    93541 ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct
    93601 gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca
    93661 tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt
    93721 agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc
    93781 ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct
    93841 tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat
    93901 tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata
    93961 gcaaccatct gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt
    94021 tttgtttgag ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa
    94081 gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct
    94141 tcaacagata aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact
    94201 tccttgacag ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta
    94261 gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca
    94321 aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca
    94381 ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca
    94441 gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat
    94501 cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga
    94561 gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat
    94621 ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc attttcagct
    94681 ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt
    94741 tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc
    94801 aaaaatgaga attttttttt tataacatca aatccacgta gatttttaca aacaaaaata
    94861 aataaagtcg gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc
    94921 agtcttctaa aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca
    94981 ttcactaaac atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata
    95041 gaattatctg ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc
    95101 tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg
    95161 tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt
    95221 ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag
    95281 ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa
    95341 aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat
    95401 ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt
    95461 ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa
    95521 tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt
    95581 aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt
    95641 ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa
    95701 tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt
    95761 tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag
    95821 caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt
    95881 tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa
    95941 gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag
    96001 ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata
    96061 aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg
    96121 ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg
    96181 ccagaaaaat tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca
    96241 attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg
    96301 cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc
    96361 tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta
    96421 actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag
    96481 tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg
    96541 gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca
    96601 gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga
    96661 agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt
    96721 ggacatctac gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta
    96781 ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc
    96841 cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag
    96901 cattgttctc gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa
    96961 aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc
    97021 ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac
    97081 ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac
    97141 tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac
    97201 tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag
    97261 cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga
    97321 ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca
    97381 aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata
    97441 cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt
    97501 tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct
    97561 cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa
    97621 aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta
    97681 cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta
    97741 tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct
    97801 cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac
    97861 aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt
    97921 gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta
    97981 tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg
    98041 aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg
    98101 ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca
    98161 ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg
    98221 atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg
    98281 gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt
    98341 ctgttactga gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc
    98401 gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac
    98461 tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag
    98521 aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat ccacactttt
    98581 ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg
    98641 ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc
    98701 tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg
    98761 agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat
    98821 tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac
    98881 tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg
    98941 ggtccattac agattcagct actttaggag attacgaaac accagctgta gaaaatatgg
    99001 attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa
    99061 atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga
    99121 cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg
    99181 ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata
    99241 tgtctatcgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta
    99301 tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta
    99361 aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg
    99421 gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc
    99481 ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa
    99541 aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt
    99601 atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt
    99661 ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta
    99721 ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc
    99781 tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg
    99841 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg
    99901 ccctgcatca agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt
    99961 tggacttatt accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag
   100021 ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt
   100081 ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa
   100141 aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc
   100201 ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg
   100261 tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc
   100321 atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct
   100381 ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt
   100441 atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa
   100501 ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa
   100561 aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg
   100621 tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt
   100681 tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc
   100741 tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg
   100801 tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca
   100861 gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc
   100921 ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct
   100981 tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga
   101041 aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct
   101101 attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt
   101161 acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga
   101221 cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat
   101281 cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag
   101341 cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg
   101401 aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt
   101461 ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa
   101521 aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct
   101581 gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat
   101641 aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa
   101701 aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg
   101761 acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt
   101821 attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg
   101881 atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag
   101941 aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa
   102001 aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt
   102061 tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg
   102121 tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga
   102181 tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc
   102241 ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt
   102301 tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga
   102361 ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga
   102421 actccccatt ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt
   102481 aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca
   102541 aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa
   102601 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
   102661 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
   102721 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
   102781 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
   102841 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
   102901 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
   102961 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
   103021 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
   103081 tttgttcacg ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat
   103141 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
   103201 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
   103261 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
   103321 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
   103381 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
   103441 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
   103501 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
   103561 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
   103621 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
   103681 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
   103741 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
   103801 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
   103861 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
   103921 acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag
   103981 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
   104041 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
   104101 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
   104161 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
   104221 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
   104281 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
   104341 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
   104401 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
   104461 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
   104521 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
   104581 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
   104641 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
   104701 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
   104761 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
   104821 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
   104881 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
   104941 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
   105001 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
   105061 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
   105121 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
   105181 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
   105241 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
   105301 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
   105361 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
   105421 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
   105481 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
   105541 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
   105601 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
   105661 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
   105721 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
   105781 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
   105841 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
   105901 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
   105961 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
   106021 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
   106081 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
   106141 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
   106201 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
   106261 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
   106321 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
   106381 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
   106441 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
   106501 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
   106561 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
   106621 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
   106681 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
   106741 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
   106801 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
   106861 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
   106921 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
   106981 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
   107041 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
   107101 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
   107161 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
   107221 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
   107281 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
   107341 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
   107401 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
   107461 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
   107521 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
   107581 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
   107641 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
   107701 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
   107761 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
   107821 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
   107881 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
   107941 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
   108001 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
   108061 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
   108121 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
   108181 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
   108241 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
   108301 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
   108361 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
   108421 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
   108481 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
   108541 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
   108601 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
   108661 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
   108721 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
   108781 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
   108841 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
   108901 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
   108961 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
   109021 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
   109081 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
   109141 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
   109201 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
   109261 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
   109321 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
   109381 aatttcagtc atttttttgt atcttctcca ttatagttgg atataccaat atataatgct
   109441 tttccttgtc ttacaagttg catcaacgct tctgctgttt cttccaaagg tgtatcaggg
   109501 tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg gaaactttgg
   109561 tcacaacttg atatgatgga tttacgagag ccccattccc catatggccc ttcccacata
   109621 tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta
   109681 aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg
   109741 tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata
   109801 ggatcataat caccaaaatt ttgccataat ccgagaggag agataaaatc ggtaatttta
   109861 aacctgaatg accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat
   109921 acaatttttc tcctcctcat caaatctttt taaaacgctt tcttgttttc tatttttagt
   109981 ttactacatt ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat ttttttgcca
   110041 tttgtttttt actcgttgat agatcagaaa ttgattacgt gcttgctgga tttttttata
   110101 gcgtttcggt tgttttttgt aaaattgctg atgatagttt tctgctggcc aaaacgtacc
   110161 ggctggttcg atttttgtga cgattggttg gtggaaagtt cctgaactgt ctagtttctg
   110221 ttttgattgt tccgctaatt ctttttgtcg ttcgtcttga taaaaaatca ctggacgata
   110281 ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa tttgccaata
   110341 gattgtcagc aactcgctat aacttatttc tcttgtgtca taaatgatct ccacagcttc
   110401 cacatgacct gtgtaaccac cacttacttg atcataattc ggtttttcaa catgtccgcc
   110461 cgtgtatcct gacaaaacag ataggatccc ttttttcgat tcaaacggct cgaccataca
   110521 ccagaaacat ccccctgcaa atactgcacg ttcttgatag tcagcatctt gcatggcatg
   110581 cttggaaaag tcatattttt gctccttatc aaatccttcc gtaatcaata aataaaagtc
   110641 cattacatca ggcgtcagat tcattcggat agctaatgga cgtagtgtta cttctagttt
   110701 tgataactgt tctttaacgg atatattttc tcttgtgtca ttctttgtct gaatcaatac
   110761 ttttcgttcc caatctcgtg tattcggatt caaaatcaga ttataaagtg tttgaatgaa
   110821 tatttgttcc tcatccatag attctcctcc tttccgaaga agacaagcta gtgactttag
   110881 ctgtaattta tcataaagtg aggaaataat cgattgaatc gtatttattc aaagaagata
   110941 agcagcttca gatgatataa gatgatcttt atcttaaaaa gcatactcta tggtttggtt
   111001 taactaaaaa acgacctctg aaaaaaggga tgttctccaa ttctcagaag tcgagtaaag
   111061 atgattcttt tttatgagcg ataataaact tcatccaaaa acagaccttg tgcagggacg
   111121 gtttctcctg catgttgccg cactttgctt tcaaatattt catcgattgt ttctatcggc
   111181 atggcacctg tgccgatttc tagtaatgtc cccatgataa tccgcaccat tttatataag
   111241 aatccgtctc cgacaaaagt aaaatgaagc atttctccct ttcgttcgat agataattca
   111301 tcaatcgtac gcactgtgga tttttttgat tttttcaatg cagaaaaacc aatgaaatca
   111361 tgtgtaccca ctaatttttc gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg
   111421 aaataaaagc tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca aacataatag
   111481 ctgtattttt tgcccaccgc attatatctt gaatggaagc gctcaggcat ttcttcgatt
   111541 cttttgatga caatatcccg cggaaggtaa cgattcataa agtcgatcat ttctgccagt
   111601 ccaatttctg tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt gccagcatct
   111661 gttcttcctg atccaataat ttctatctct tcatttgtca tctgatgcaa tactttttcg
   111721 atttttcctt gaatggtttt atcgtcatct ccaagtcttt gccatcctga atatcttttt
   111781 ccgtcatatt cgattgtcaa cttgatgttt ctcatatttt agtggctcct ctgtcttcca
   111841 tttggtcttc tctctacctt tttaagattg tagcacaaaa tatagctcct tgcttctcta
   111901 agacagaagc tatacaagaa aactgtggaa atatatattt cagaaaaaca aaaaacagcc
   111961 accggatgaa gtatccgctg actgttcaat ttgacggctc aagttttact gtttgagatg
   112021 caagatactc aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa
   112081 acctatttta ttcagtaatg gccaagaatt tatagaagga attcttgcca gtccataaat
   112141 cgccgcaaaa agcaaactga tcacagacag tccaaggtat aagtggctta cgtccatttg
   112201 cccatttttt cgatagatca agaaaagaag aactggaaac aataaaaaac cactgtaacc
   112261 taaacctgat ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt
   112321 gatactgaac aaacctgtaa agatacaatc acctatacca tagaacgcga tggctgctgt
   112381 aactagggta gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga
   112441 caggatgaaa aagatccctg aaaccacaga ccaaataaga aacgcttttc ttacaggact
   112501 ggaaacctct ccaaatacac tgatcacgct cttcatttga tctaattcag gataatacaa
   112561 tcctaatacg tacggagtca aaaaatcact cagcacgcct aataataaaa agaaaaaacc
   112621 aaatttacgt aaaaaatcca tgatacgcct ccaataacaa ttacctatgc ttttaattgt
   112681 tattataaac gactttatct tgatttaaca gaaacctcta tttacacgaa catctatata
   112741 actcattaaa aactcgaatc attttaaaaa tactaaatat ctaacgttca acgttcactt
   112801 aatccatcta aacaatcttt caatttgatt ttttccccac ttcttttacg cgcgcctagc
   112861 tctcctattg cagtaaacaa cagatcactt gacgaattta aggcagtttc cattgaatcc
   112921 tggataacac cgataataaa tccaatacca acgatttgca tcgcgacatc attagagata
   112981 ccaaacaaac tacatgctaa tggaatcaat aacagtgaac cgcccgcaac acctgaagct
   113041 ccacaagcag ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa tggtgcttca
   113101 atccctaaag aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc
   113161 atattgatcg tagcacctaa cgggatcgat atggaataag actcttttgt caatttcaat
   113221 ctttcagcca acatcatgtt tatcgggata tttgcggcag aacttcgggt aaagaaagcg
   113281 ggaagtgcgc tttctttcaa acaaaagaaa atcaatggat agggattttc tttaataaac
   113341 aaaaacgtga ggaatgggta cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca
   113401 agtaaaagct gcagatactc tgccaatcca gcgattccag tcgtagcaat tgattgataa
   113461 accaatccta aaatccctat cggagcaaat gcaataatca cttgtacgac ttgtgacaac
   113521 gctatagata tctgatccac caactctttt gtcggttctg aacgtgctcg aaaagctagt
   113581 cccaacaata cagcccagaa taaaacgcca aggtaattcg catcaataag agattgaaca
   113641 ggattttgtg tgacattcgt caacatagta gataaaacac tttccaaact ttgcggtgct
   113701 ttttcagata ctgtttcttg taaagtaata ggaacagtaa aaattgaact tacaatgact
   113761 gctacaagtg cagaaagcaa tgttcccact aaatataaga tcaaaatagg cttcacgaat
   113821 gtcttattcc caattttatg cttggcaatt gacgccatcg tcaagaaaaa aaccagaata
   113881 ggtgcgattg ccttcaagca actgacaaac aacgtcccca atattgaaat aaatgtccac
   113941 gtggggaaaa agataccaaa caatattccg actaaaatac caacaacaat acgttgaact
   114001 aacgacatat ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt taacaatcat
   114061 tgtatcattt tatctaaaga aactaaaggt gcaattttct aaaaattcta ataatacaat
   114121 tggcaattca tgctcttgca acattcaatt ttagtattgg agggtttatt caatctctaa
   114181 agaagtcgag agaaaactat ttatcgttat tattatagtt atccaagtaa aacgtaacta
   114241 agatgaagga cgaaactaga aatttcatcc ttcatcttaa ttcttagaca tagcaatcaa
   114301 ttaatatttt ccactgagtc tcttctttct ctcactctca ctggtttact tacccataaa
   114361 cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttaaag taaagataat
   114421 tatcggaatt cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc
   114481 cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg
   114541 tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc
   114601 caccaatagc catcttccac tccccgttca aaataacttg agggacttta agaccatata
   114661 agtccacact catttgattc gtatagcttt tgtattgttg tcccattgtt tggccccatg
   114721 tagccataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcgactt
   114781 gttcgctgct ttctgaagat gaagcctcta cagaagaact tgaagtagat gtttgagttg
   114841 tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttcc gtacttgctg
   114901 cagttggctt gctctctgtg ctagttgttg tagtagttgc aattgttgct ttcgctgttt
   114961 cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac aaaaaagcta
   115021 gaccaattgt tactaaaatc ctttttttaa attaaaaaac tcctttcgat tactcccact
   115081 tctttattct atcaaataag caatgtgatt ttattgattt tttgtatttt ctcatccatc
   115141 ttttaaacct tttaaaaact aagaagcacc cgctgatgtt gtctgtaata catcagcggg
   115201 tgcttctttt attttaatct tcgcgatttt ccaatttacc tcacgcttta tatttgtatt
   115261 tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt
   115321 tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa aagttcaaca
   115381 agcaattgtt tcaagccata tttttcttct ttttcagcta atttgattgc ccgtgtgata
   115441 aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga ggctggtaaa
   115501 tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc taaagtagac
   115561 ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca tctcgcttct
   115621 tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc caaaacatac
   115681 aattgtgctt ggatcaattt catttcttct aatttaaaat ttgccaatac atgccccacc
   115741 atggcgccag cagttggttt atgatgatcg atatctgctt gttttgtttc ttgagcaaat
   115801 ttttcttcca ctatattcac tgatttacgc ctcttttact tttgttgatt ggacttcata
   115861 acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg gatcaaagtt
   115921 tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag gctgttgaga
   115981 aaccgcatgt tctatttttt gcatacagct tggacaagct agtgtatcta attgcataat
   116041 catttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct agattacagc
   116101 ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc
   116161 aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt cgtgaacgaa
   116221 catcccacta gccatgtaaa taaacccggc aaataagccg atgagtaaaa ataagacgac
   116281 accaatggca atcactacat tttcacgggt attcaaaaca gttttcttag ccaaacggta
   116341 agcatgaacc aacgatccaa aactagattg catcaagaca acgtcactcg tttcgatagc
   116401 gacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag agggagaatc
   116461 attgattcca tctccaatga aagcaacgtg caatccttgt tcttggtatt ttttcacaaa
   116521 ttgaaccttt tcatctggca ataattcagc atggacttca tcaatgccta acatgtcagc
   116581 tacataatca gcagtcatct gattatcacc tgtcagcatc actaaatgtt ttgctcctgc
   116641 ttgtttcaac atagctaatt gttcagcgac ttctggtcga atcacatctg acacaccaat
   116701 gatttgagtg atttttttgt cgattgctac aataatgact gagctgcctc tttgttgtaa
   116761 ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg caatcaattt
   116821 ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt gtcccttgat
   116881 cgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct gagcataatg
   116941 gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca ctaaagctaa
   117001 gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag ttcctttggt
   117061 caatgtaccg gttttatcaa agacaaaagt atcgattttg gaaaaagtat ccattgcttc
   117121 accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta cattcgaaac
   117181 aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga tggctaaacg
   117241 aaaatcacga ctgatcagac caacaagcaa cgcaataagt aaaacggttg gagtataata
   117301 ctgagaaaat tgatcaatga atttttccgc atgtgatttg gtatcttgtg cttcttcgac
   117361 taattctata attttggcaa aggtcgtatc atctccgacc ttagttgctt cgatcgtcac
   117421 gtagccatct gctaaaatcg ttccagaaaa tacgttatct ccactgattt tgacgacttg
   117481 tcgtgattca cccgtcactg cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc
   117541 gtccacagga atagctccac cagtttttac taagacgata tcgccttctt ccacgtcatc
   117601 gatttccact tcttcggtcg ttccatcttc ttggacaacg ctggcagtcg atggggccat
   117661 ctcggtcaaa gccttgattg attcccgcgt tttggtcaat gttttttgtt ccagataact
   117721 accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga tcataaaggc
   117781 accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt ttactgcctg
   117841 atacgcatgg attataatag gtatgaccgc aataatagaa gctatggcta gagcaatgtt
   117901 atagaacaag acaaatccag taaatttccc gataaaacca aaaataatca acacagctga
   117961 taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca tctttctccc
   118021 tcttttcctt ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga
   118081 ccactatcaa gtttattcaa aaaggcggtt ttcttttggt atactgaatt cctacagtca
   118141 ttaaacgatc atatagaaaa aggaagcgcc gaaacgcttc cttaggtagt tctatttttt
   118201 tattcaaaga taagatgctt tgtcatctta cgactgaaga acaacaatca aaaaagggca
   118261 gcagccactc attcacaaga aatatcgttt tttgatatgg gagttgaagc agtggacaaa
   118321 tatctctact tattagctgg gaataagatc caaaaaagct taatggattt tatccaagaa
   118381 ttggaatgta ctttccacaa aaaattcact cattccattc ttttaaaact tttgattcat
   118441 acagcgtgtc taattgagcg tacattgata aacggtcatg aacttaaaat tatatcagaa
   118501 gatgacacaa gaccttctca tgaaacaatt tttcatgtaa aaaaggcctt caaaaatatt
   118561 gaaacagaat ttgggatcac tgtttcttat gatgaatgtt tctttatcta tgatattatc
   118621 gcatctaaat aatctttttt tatgtaaacg agtattttgg gagcgtcaat aattttgtgt
   118681 aaataaattg tcctcctgca aaataattag ttactcagta aacattgaaa ctaatgtatc
   118741 ggttac
//
LOCUS       DACECB010000002       100948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECB010000002 DACECB010000000
VERSION     DACECB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.002"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWO04_000107"
     CDS             212..604
                     /locus_tag="IWO04_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0211750.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWO04_000108"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWO04_000108"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0211751.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWO04_000109"
     CDS             3165..4775
                     /locus_tag="IWO04_000109"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0211752.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWO04_000110"
     CDS             5067..5936
                     /locus_tag="IWO04_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0211753.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWO04_000111"
     CDS             6101..7372
                     /locus_tag="IWO04_000111"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0211754.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWO04_000112"
     CDS             7369..8664
                     /locus_tag="IWO04_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0211755.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWO04_000113"
     CDS             8763..9026
                     /locus_tag="IWO04_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0211756.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWO04_000114"
     CDS             9471..9920
                     /locus_tag="IWO04_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0211757.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWO04_000115"
     CDS             9977..11395
                     /locus_tag="IWO04_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0211758.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWO04_000116"
     CDS             11395..12255
                     /locus_tag="IWO04_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0211759.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWO04_000117"
     CDS             12429..13202
                     /locus_tag="IWO04_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0211760.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWO04_000118"
     CDS             13349..13714
                     /locus_tag="IWO04_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0211761.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWO04_000119"
     CDS             complement(13862..15070)
                     /locus_tag="IWO04_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0211762.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWO04_000120"
     CDS             complement(15089..15367)
                     /locus_tag="IWO04_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211763.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWO04_000121"
     CDS             15560..16216
                     /locus_tag="IWO04_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0211764.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWO04_000122"
     CDS             complement(16658..17455)
                     /locus_tag="IWO04_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0211765.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWO04_000123"
     CDS             complement(17452..18636)
                     /locus_tag="IWO04_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0211766.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWO04_000124"
     CDS             complement(18661..19908)
                     /locus_tag="IWO04_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0211767.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO04_000125"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWO04_000125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0211768.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWO04_000126"
     CDS             21888..22202
                     /locus_tag="IWO04_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0211769.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWO04_000127"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWO04_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0211770.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWO04_000128"
     CDS             22887..23531
                     /locus_tag="IWO04_000128"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0211771.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWO04_000129"
     CDS             23546..23875
                     /locus_tag="IWO04_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211772.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWO04_000130"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWO04_000130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0211773.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWO04_000131"
     CDS             24863..25687
                     /locus_tag="IWO04_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0211774.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWO04_000132"
     CDS             25680..26027
                     /locus_tag="IWO04_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0211775.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO04_000133"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWO04_000133"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0211776.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWO04_000134"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWO04_000134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0211777.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO04_000135"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWO04_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0211778.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO04_000136"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWO04_000136"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0211779.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWO04_000137"
     CDS             31684..32451
                     /locus_tag="IWO04_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211780.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWO04_000138"
     CDS             32441..34483
                     /locus_tag="IWO04_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0211781.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKIADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWO04_000139"
     CDS             34764..34913
                     /locus_tag="IWO04_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0211782.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWO04_000140"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWO04_000140"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0211783.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWO04_000141"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWO04_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0211784.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWO04_000142"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWO04_000142"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0211785.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWO04_000143"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWO04_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0211786.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWO04_000144"
     CDS             39330..40412
                     /locus_tag="IWO04_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0211787.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWO04_000145"
     CDS             40412..41215
                     /locus_tag="IWO04_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0211788.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPMGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWO04_000146"
     CDS             41285..42025
                     /locus_tag="IWO04_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0211789.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWO04_000147"
     CDS             42036..43040
                     /locus_tag="IWO04_000147"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0211790.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWO04_000148"
     CDS             complement(43085..44929)
                     /locus_tag="IWO04_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211791.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45658
                     /locus_tag="IWO04_000149"
                     /pseudo
     CDS             45141..45658
                     /locus_tag="IWO04_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            45786..46469
                     /locus_tag="IWO04_000150"
     CDS             45786..46469
                     /locus_tag="IWO04_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0211792.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="IWO04_000151"
     tRNA            46778..46851
                     /locus_tag="IWO04_000151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="IWO04_000152"
     CDS             47010..47369
                     /locus_tag="IWO04_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0211793.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="IWO04_000153"
     CDS             47492..49123
                     /locus_tag="IWO04_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0211794.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="IWO04_000154"
     CDS             complement(49185..49826)
                     /locus_tag="IWO04_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0211795.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="IWO04_000155"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="IWO04_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0211796.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="IWO04_000156"
     CDS             50443..50787
                     /locus_tag="IWO04_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0211797.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="IWO04_000157"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="IWO04_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0211798.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51500..54334
                     /locus_tag="IWO04_000158"
     CDS             51500..54334
                     /locus_tag="IWO04_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0211799.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="IWO04_000159"
     CDS             54525..55016
                     /locus_tag="IWO04_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0211800.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="IWO04_000160"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="IWO04_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0211801.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="IWO04_000161"
     CDS             56141..56944
                     /locus_tag="IWO04_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0211802.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="IWO04_000162"
     CDS             56965..57879
                     /locus_tag="IWO04_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0211803.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="IWO04_000163"
     CDS             57962..58336
                     /locus_tag="IWO04_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0211804.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="IWO04_000164"
     CDS             58475..58720
                     /locus_tag="IWO04_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0211805.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="IWO04_000165"
     CDS             58892..59956
                     /locus_tag="IWO04_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0211806.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="IWO04_000166"
     CDS             60202..60627
                     /locus_tag="IWO04_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0211807.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="IWO04_000167"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="IWO04_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0211808.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="IWO04_000168"
     CDS             61166..62020
                     /locus_tag="IWO04_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0211809.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="IWO04_000169"
     CDS             62021..63358
                     /locus_tag="IWO04_000169"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0211810.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="IWO04_000170"
     CDS             63363..63587
                     /locus_tag="IWO04_000170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0211811.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="IWO04_000171"
     CDS             63589..64479
                     /locus_tag="IWO04_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0211812.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="IWO04_000172"
     CDS             64488..65306
                     /locus_tag="IWO04_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0211813.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="IWO04_000173"
     CDS             65413..65862
                     /locus_tag="IWO04_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0211814.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="IWO04_000174"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="IWO04_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0211815.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="IWO04_000175"
     CDS             complement(67617..67733)
                     /locus_tag="IWO04_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0211816.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="IWO04_000176"
     CDS             complement(67806..68762)
                     /locus_tag="IWO04_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0211817.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="IWO04_000177"
     CDS             68933..69301
                     /locus_tag="IWO04_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0211818.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="IWO04_000178"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="IWO04_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0211819.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="IWO04_000179"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="IWO04_000179"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0211820.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="IWO04_000180"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="IWO04_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0211821.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="IWO04_000181"
     CDS             71289..73481
                     /locus_tag="IWO04_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0211822.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="IWO04_000182"
     CDS             73507..74469
                     /locus_tag="IWO04_000182"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0211823.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="IWO04_000183"
     CDS             74483..75844
                     /locus_tag="IWO04_000183"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0211824.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="IWO04_000184"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="IWO04_000184"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0211825.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="IWO04_000185"
     CDS             76973..78193
                     /locus_tag="IWO04_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0211826.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="IWO04_000186"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="IWO04_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0211827.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="IWO04_000187"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="IWO04_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0211828.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="IWO04_000188"
     CDS             80955..81632
                     /locus_tag="IWO04_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0211829.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="IWO04_000189"
     CDS             81646..82245
                     /locus_tag="IWO04_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0211830.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="IWO04_000190"
     CDS             82260..82547
                     /locus_tag="IWO04_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0211831.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="IWO04_000191"
     CDS             82596..83378
                     /locus_tag="IWO04_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0211832.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="IWO04_000192"
     CDS             83399..84106
                     /locus_tag="IWO04_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0211833.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="IWO04_000193"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="IWO04_000193"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0211834.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="IWO04_000194"
     CDS             complement(87215..88741)
                     /locus_tag="IWO04_000194"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0211835.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="IWO04_000195"
     CDS             complement(88756..89403)
                     /locus_tag="IWO04_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0211836.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="IWO04_000196"
     CDS             complement(89587..89916)
                     /locus_tag="IWO04_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0211837.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="IWO04_000197"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="IWO04_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0211838.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="IWO04_000198"
     CDS             90837..91850
                     /locus_tag="IWO04_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0211839.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="IWO04_000199"
     CDS             91850..93127
                     /locus_tag="IWO04_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0211840.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="IWO04_000200"
     CDS             93190..95892
                     /locus_tag="IWO04_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0211841.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="IWO04_000201"
     CDS             96044..96361
                     /locus_tag="IWO04_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0211842.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="IWO04_000202"
     CDS             96391..96711
                     /locus_tag="IWO04_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0211843.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="IWO04_000203"
     CDS             96819..98279
                     /locus_tag="IWO04_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0211844.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="IWO04_000204"
     CDS             98347..98568
                     /locus_tag="IWO04_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211845.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="IWO04_000205"
     CDS             98599..98781
                     /locus_tag="IWO04_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0211846.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="IWO04_000206"
     CDS             98781..99194
                     /locus_tag="IWO04_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0211847.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="IWO04_000207"
     CDS             99317..100498
                     /locus_tag="IWO04_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0211848.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        32980 a        17282 c        21423 g        29263 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaattgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctatgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctcccgattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaagg
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cggaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaccg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattc
//
LOCUS       DACECB010000003        82787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECB010000003 DACECB010000000
VERSION     DACECB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.003"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            69..383
                     /locus_tag="IWO04_000208"
     CDS             69..383
                     /locus_tag="IWO04_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211849.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            376..870
                     /locus_tag="IWO04_000209"
     CDS             376..870
                     /locus_tag="IWO04_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211850.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            911..1261
                     /locus_tag="IWO04_000210"
     CDS             911..1261
                     /locus_tag="IWO04_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211851.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
     gene            1509..2438
                     /locus_tag="IWO04_000211"
     CDS             1509..2438
                     /locus_tag="IWO04_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211852.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            2607..3932
                     /locus_tag="IWO04_000212"
     CDS             2607..3932
                     /locus_tag="IWO04_000212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211853.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            4032..4850
                     /locus_tag="IWO04_000213"
     CDS             4032..4850
                     /locus_tag="IWO04_000213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211854.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(5289..6434)
                     /locus_tag="IWO04_000214"
     CDS             complement(5289..6434)
                     /locus_tag="IWO04_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0211855.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(6495..7142)
                     /locus_tag="IWO04_000215"
     CDS             complement(6495..7142)
                     /locus_tag="IWO04_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0211856.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            7336..7629
                     /locus_tag="IWO04_000216"
     CDS             7336..7629
                     /locus_tag="IWO04_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211857.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            7673..7984
                     /locus_tag="IWO04_000217"
     CDS             7673..7984
                     /locus_tag="IWO04_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211858.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            7981..8605
                     /locus_tag="IWO04_000218"
                     /pseudo
     CDS             7981..8605
                     /locus_tag="IWO04_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            8905..9267
                     /locus_tag="IWO04_000219"
     CDS             8905..9267
                     /locus_tag="IWO04_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211859.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            9304..10152
                     /locus_tag="IWO04_000220"
     CDS             9304..10152
                     /locus_tag="IWO04_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0211860.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            10142..11617
                     /locus_tag="IWO04_000221"
     CDS             10142..11617
                     /locus_tag="IWO04_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ0211861.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            11883..12290
                     /locus_tag="IWO04_000222"
     CDS             11883..12290
                     /locus_tag="IWO04_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ0211862.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            12293..12508
                     /locus_tag="IWO04_000223"
     CDS             12293..12508
                     /locus_tag="IWO04_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211863.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            12512..12892
                     /locus_tag="IWO04_000224"
     CDS             12512..12892
                     /locus_tag="IWO04_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0211864.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            13026..13181
                     /locus_tag="IWO04_000225"
     CDS             13026..13181
                     /locus_tag="IWO04_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ0211865.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            13250..13723
                     /locus_tag="IWO04_000226"
     CDS             13250..13723
                     /locus_tag="IWO04_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0211866.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            13720..15414
                     /locus_tag="IWO04_000227"
     CDS             13720..15414
                     /locus_tag="IWO04_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0211867.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            15380..15565
                     /locus_tag="IWO04_000228"
     CDS             15380..15565
                     /locus_tag="IWO04_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211868.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            15569..16744
                     /locus_tag="IWO04_000229"
     CDS             15569..16744
                     /locus_tag="IWO04_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0211869.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            16737..18260
                     /locus_tag="IWO04_000230"
     CDS             16737..18260
                     /locus_tag="IWO04_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0211870.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            18316..18600
                     /locus_tag="IWO04_000231"
     CDS             18316..18600
                     /locus_tag="IWO04_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0211871.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            18587..18922
                     /locus_tag="IWO04_000232"
     CDS             18587..18922
                     /locus_tag="IWO04_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0211872.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            19217..19843
                     /locus_tag="IWO04_000233"
     CDS             19217..19843
                     /locus_tag="IWO04_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211873.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            20585..21421
                     /locus_tag="IWO04_000234"
     CDS             20585..21421
                     /locus_tag="IWO04_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0211874.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            21486..21920
                     /locus_tag="IWO04_000235"
     CDS             21486..21920
                     /locus_tag="IWO04_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0211875.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(21988..23310)
                     /locus_tag="IWO04_000236"
     CDS             complement(21988..23310)
                     /locus_tag="IWO04_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0211876.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(23464..25104)
                     /locus_tag="IWO04_000237"
     CDS             complement(23464..25104)
                     /locus_tag="IWO04_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0211877.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25434..25904
                     /locus_tag="IWO04_000238"
     CDS             25434..25904
                     /locus_tag="IWO04_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0211878.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            26533..27636
                     /locus_tag="IWO04_000239"
     CDS             26533..27636
                     /locus_tag="IWO04_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0211879.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            27920..28600
                     /locus_tag="IWO04_000240"
     CDS             27920..28600
                     /locus_tag="IWO04_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0211880.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            28690..29724
                     /locus_tag="IWO04_000241"
     CDS             28690..29724
                     /locus_tag="IWO04_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0211881.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            29738..30073
                     /locus_tag="IWO04_000242"
     CDS             29738..30073
                     /locus_tag="IWO04_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211882.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            30057..32537
                     /locus_tag="IWO04_000243"
     CDS             30057..32537
                     /locus_tag="IWO04_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211883.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            32593..34152
                     /locus_tag="IWO04_000244"
     CDS             32593..34152
                     /locus_tag="IWO04_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0211884.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            34149..35513
                     /locus_tag="IWO04_000245"
     CDS             34149..35513
                     /locus_tag="IWO04_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0211885.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            35684..36754
                     /locus_tag="IWO04_000246"
     CDS             35684..36754
                     /locus_tag="IWO04_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0211886.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            36769..37437
                     /locus_tag="IWO04_000247"
     CDS             36769..37437
                     /locus_tag="IWO04_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211887.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            37457..38035
                     /locus_tag="IWO04_000248"
     CDS             37457..38035
                     /locus_tag="IWO04_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0211888.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            38438..39349
                     /locus_tag="IWO04_000249"
     CDS             38438..39349
                     /locus_tag="IWO04_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0211889.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(39605..40522)
                     /locus_tag="IWO04_000250"
     CDS             complement(39605..40522)
                     /locus_tag="IWO04_000250"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0211890.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            40724..41161
                     /gene="zapA"
                     /locus_tag="IWO04_000251"
     CDS             40724..41161
                     /gene="zapA"
                     /locus_tag="IWO04_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0211891.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            41219..41749
                     /locus_tag="IWO04_000252"
     CDS             41219..41749
                     /locus_tag="IWO04_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0211892.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            41881..44241
                     /locus_tag="IWO04_000253"
     CDS             41881..44241
                     /locus_tag="IWO04_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0211893.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            44328..44642
                     /gene="trxA"
                     /locus_tag="IWO04_000254"
     CDS             44328..44642
                     /gene="trxA"
                     /locus_tag="IWO04_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0211894.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            44824..46602
                     /gene="uvrC"
                     /locus_tag="IWO04_000255"
     CDS             44824..46602
                     /gene="uvrC"
                     /locus_tag="IWO04_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0211895.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            46747..47646
                     /locus_tag="IWO04_000256"
     CDS             46747..47646
                     /locus_tag="IWO04_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211896.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            47639..48901
                     /locus_tag="IWO04_000257"
     CDS             47639..48901
                     /locus_tag="IWO04_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0211897.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            49064..50962
                     /locus_tag="IWO04_000258"
     CDS             49064..50962
                     /locus_tag="IWO04_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0211898.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(51261..51980)
                     /locus_tag="IWO04_000259"
     CDS             complement(51261..51980)
                     /locus_tag="IWO04_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0211899.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            52317..52649
                     /locus_tag="IWO04_000260"
     CDS             52317..52649
                     /locus_tag="IWO04_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211900.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            52636..54639
                     /locus_tag="IWO04_000261"
     CDS             52636..54639
                     /locus_tag="IWO04_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0211901.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            54658..55128
                     /locus_tag="IWO04_000262"
     CDS             54658..55128
                     /locus_tag="IWO04_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0211902.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            55149..55733
                     /locus_tag="IWO04_000263"
     CDS             55149..55733
                     /locus_tag="IWO04_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0211903.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            55748..56734
                     /locus_tag="IWO04_000264"
     CDS             55748..56734
                     /locus_tag="IWO04_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0211904.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            56724..57035
                     /locus_tag="IWO04_000265"
     CDS             56724..57035
                     /locus_tag="IWO04_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0211905.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            57057..58838
                     /locus_tag="IWO04_000266"
     CDS             57057..58838
                     /locus_tag="IWO04_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0211906.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            58831..60207
                     /locus_tag="IWO04_000267"
     CDS             58831..60207
                     /locus_tag="IWO04_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0211907.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            60214..60849
                     /locus_tag="IWO04_000268"
     CDS             60214..60849
                     /locus_tag="IWO04_000268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0211908.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            60879..62234
                     /locus_tag="IWO04_000269"
     CDS             60879..62234
                     /locus_tag="IWO04_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0211909.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(62426..63820)
                     /locus_tag="IWO04_000270"
     CDS             complement(62426..63820)
                     /locus_tag="IWO04_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0211910.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            63912..64883
                     /locus_tag="IWO04_000271"
     CDS             63912..64883
                     /locus_tag="IWO04_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0211911.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            64880..65662
                     /locus_tag="IWO04_000272"
     CDS             64880..65662
                     /locus_tag="IWO04_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0211912.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(65831..66202)
                     /locus_tag="IWO04_000273"
     CDS             complement(65831..66202)
                     /locus_tag="IWO04_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0211913.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            66336..66779
                     /locus_tag="IWO04_000274"
     CDS             66336..66779
                     /locus_tag="IWO04_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0211914.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(66822..67631)
                     /gene="yidA"
                     /locus_tag="IWO04_000275"
     CDS             complement(66822..67631)
                     /gene="yidA"
                     /locus_tag="IWO04_000275"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0211915.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(67650..69017)
                     /locus_tag="IWO04_000276"
     CDS             complement(67650..69017)
                     /locus_tag="IWO04_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0211916.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(69001..69831)
                     /locus_tag="IWO04_000277"
     CDS             complement(69001..69831)
                     /locus_tag="IWO04_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0211917.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            69994..70431
                     /locus_tag="IWO04_000278"
     CDS             69994..70431
                     /locus_tag="IWO04_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0211918.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            70507..71127
                     /locus_tag="IWO04_000279"
     CDS             70507..71127
                     /locus_tag="IWO04_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0211919.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            71316..71822
                     /locus_tag="IWO04_000280"
     CDS             71316..71822
                     /locus_tag="IWO04_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0211920.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            71839..72738
                     /locus_tag="IWO04_000281"
     CDS             71839..72738
                     /locus_tag="IWO04_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0211921.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            72842..73726
                     /locus_tag="IWO04_000282"
     CDS             72842..73726
                     /locus_tag="IWO04_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0211922.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(73918..73989)
                     /locus_tag="IWO04_000283"
     tRNA            complement(73918..73989)
                     /locus_tag="IWO04_000283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73954..73956),aa:Arg,seq:ccg)
     gene            74049..75383
                     /gene="rpoN"
                     /locus_tag="IWO04_000284"
     CDS             74049..75383
                     /gene="rpoN"
                     /locus_tag="IWO04_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0211923.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            75586..76623
                     /locus_tag="IWO04_000285"
     CDS             75586..76623
                     /locus_tag="IWO04_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0211924.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            76664..77665
                     /gene="gap"
                     /locus_tag="IWO04_000286"
     CDS             76664..77665
                     /gene="gap"
                     /locus_tag="IWO04_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0211925.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            77775..78965
                     /locus_tag="IWO04_000287"
     CDS             77775..78965
                     /locus_tag="IWO04_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0211926.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            79069..79824
                     /locus_tag="IWO04_000288"
     CDS             79069..79824
                     /locus_tag="IWO04_000288"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0211927.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            79940..81238
                     /gene="eno"
                     /locus_tag="IWO04_000289"
     CDS             79940..81238
                     /gene="eno"
                     /locus_tag="IWO04_000289"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0211928.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            81637..82776
                     /locus_tag="IWO04_000290"
     CDS             81637..82776
                     /locus_tag="IWO04_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0211929.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        27920 a        13985 c        17185 g        23697 t
ORIGIN      
        1 gggtaaatct ctgagggagc aagctcccta cagactccct cccaaaaaaa gaaaggtgga
       61 agaaaaaaat gaactatcca tattttaaag tctcggcttc agaagaaact aaagaaatat
      121 tcaacaattt ttacaaccaa aacaaaggag tttttggttc caaggcaaat atgtttcgtg
      181 ttatggtttc aaatttacca gtgttagcta gtccatcaaa taataaattt aatgattctg
      241 aaagcattaa atttgaacaa aaaatttcag aactagaatc aatgataagt aatgaagtaa
      301 ttgaaaagct tgatgacatt gatcaaaagt tatcgtattc tctaaaaaac aaatacaaaa
      361 cggaggagaa aaaagatgtt taaagtttac ttcccgtttc agtggctcgt taaaggaatt
      421 cttttagtgc tactaggatt gattcttttt ggaagttatc taacaattaa agccagacat
      481 tttccagaag aaaagccaat aaaaattgta acaatagatt taaaaatagg tgaaacttat
      541 caggcagatg gggttgaact aacatttaaa aatattgaag tgaccgaaaa aagaaatgaa
      601 tttgcacaac atgatcgtgt attacaagtg acctatacac ttaaaaatga tacagaaaaa
      661 ccaatacgtc cacaacaatg gtcgattgaa aataaggatg gtgaagagct ttatgagtat
      721 gctatgatat tagataatcc agagtatatt gaaagtgaca aagaagaaac agtaaattac
      781 tattatgaat taccgattga aaggcaggac taccaaatcg tagtacgtag agcgaatggt
      841 tattttgttg gattaccctt tactttgtaa gtcttatttt gttagtggct tttaagttaa
      901 tatcattttt gtgatttttg aaaatgaaag tgaggaaatt ggaagaatgg ctgaagaaaa
      961 taaagttgtt ccgtttgaaa cattagtgat aaataaggaa gagtttgagc aactttttct
     1021 tgattttttt attcaatatg acaggattta tgattttagt tcgtcactag atttatttga
     1081 attagaagca gaaataatag aaaacagtcg agagaagaat ggtaaaatca ttatttcagt
     1141 agaaaaattc aatgaattat tctcagaaac atttgaaata gatccagact attatcaacc
     1201 acaagaagtt tttcgagagt ttagaaatca gattattcgt tcaagtaata ttgaaaattg
     1261 agtcaataaa agaagcgttg aagaaaagtt ccttctttcg acgcttcttt ttaaaagatt
     1321 ttacaaatgt cttatggaga tttgcttcaa tgactacttg tagtcatttc gtttaaaata
     1381 attatgttac acccttatgt gtcacagaga aatactgata taataagaaa taaggtatgt
     1441 aacaatagag tatacttcta tgttacatat tgattatttt gaaaaaatca tattaggaga
     1501 atataattat gagaagtgat agtaggtttt ttgtcagtaa tttacaagat gacgagctaa
     1561 taaggcagat tgattcatta ttagaaacaa taacagaatc tgataaaaga atatttttaa
     1621 attatgtaga attaacacgt catattatag agttggataa actttttaat gtgtttagat
     1681 ataatttaac taatttgctc aaacacttca caatttttac taatgattta attgaaagta
     1741 caggtgaaaa attaacggaa gaccagtatt attatcagat aaatgctttg acgataaact
     1801 taattagttc agccaaaact ttgactgaat ccattgaagt ttgtatgaaa aactttttag
     1861 cagaaaaaga ttttaaatct tttaagaata aaattctgag taagccttat gatgagcatt
     1921 tttcgtatag gtttttatta catatgagaa attattctca gcatggtcat ctgccagtaa
     1981 atattcatca acaaagagtg tactttgatt tggatgaaat tctagcaatg cctcattttg
     2041 attttaacgg acaattaaaa aaggagttag atgttttaag gaaagatata tataaccaat
     2101 ttagagattc cccacgtatc tcttatgttt atacagtagc gaaatttaat ttagtaataa
     2161 ccgaaatata tttcaattat ttaaaagaga tcaaacctat tttaataaag ttgaatgacg
     2221 agaaaaatga attactactc aataccaaat ttcagttaat taattcagat ggaagctcaa
     2281 gtgatatgct tttttatgag tttgatggtg agaattatca ctgcttcaac cgaaaggaca
     2341 acagtatatc tatgtatgct gacataaaaa gagaagttaa aaaaatatta aaagtagaag
     2401 aacaatacta taaagaaata gaaaaaagaa accagtaatt aacacagtta tcaaggtaaa
     2461 gaaaaagttc tctttctgtt tgctagttgt ggcttatgta gcaaatatca agttaatagg
     2521 aaaattaaaa ttgttgaaag gaaagaagtt gatgacatat tagtaagcaa gttgttgaat
     2581 atttatatta tcctaaaaaa ggaagaatgg aaaatatcga agaaaccttt ctgaaagtgt
     2641 tgtcaccata tattagtgat tctatcttaa gatttcagga gcaggaaaaa aattataaat
     2701 ttggctttga cataaagaac tataatgatt cgttacacgt agtatttcaa tataataaga
     2761 ctggtaagat aggaataaaa gaggtacata tatttcaaat tttcctagct aaggtcaata
     2821 ttgaagcaag taaatcaaaa ttaaactaca ctaccctttt agatacaaat gcgttgtttt
     2881 tttcgaaaaa actattaccg tatttgcata gttacgaatg ggcattaaga aaattaatct
     2941 atttattagc tccggcatat ttttctgata actgggcaga ggaatcaatt ccaaatgaaa
     3001 tcatttcgtc aataaaaaga aaacaaatac aagcaatagg aaagtttgac tcaaagaatc
     3061 ttttacaatg gatagattta tatgattttg aagaatatct ctttggagaa aattatattt
     3121 tagttacaca agaaaatgaa gagaatattg taaggtataa agaaatatct caggatcaat
     3181 tattgaagtt atttcaagat aacagtcaca ctatatctga ttcatattct ttgtggagtg
     3241 aagtatttag taaatatatg gatgttaagc tagaagaaat tcaaacagac atgagtttaa
     3301 tcagagaagg taggaatatt gtaagccata acaaagaaat agaaatggct ttataccaaa
     3361 aattaataaa gaaattgaag agatatacaa atcaactaga gaaagctttt caaaaaatat
     3421 tggctggaga tattgctggt gaggacctta gtgatatgtc agatgatttt gaaggatata
     3481 ttggaaatta ttttagtaat atcgatttag caggtgttac taaattgtca gagtctctat
     3541 cagcaatgaa agtattaaat acagattcca tgaaaaagtc gatggagtca ataggtcaaa
     3601 cattatcagt ccagcaagaa attatgcaga actttgatac tgaaggtatc agaagggcaa
     3661 cagagggaat aaatcaatct ctacaatcaa taaaaatatt aaatacagat tccatgaaga
     3721 ggtcgataga gtcaataggt caaacattat cagctttatc agctcagcga gcaatcatgc
     3781 aaaactttga tactaaaggt atcagaagtg caacagaggg aataaatcaa tctctacaat
     3841 caatgaaaat agtaaataca gattccatga aacgatcgat ggaatcaatt ggtaaaattt
     3901 catcaacaaa ccaagtattg aagtcagagt aaataaaaca ggaacgatgg caatcatgtt
     3961 accacacgaa atgctatcat ttgtagcaag tattggattt agagtaatta ttatagagag
     4021 gatgagataa aatggttcaa ggactttttg gtggagggac ttcttatgaa ggtcaatcga
     4081 tttcggacat taaggaagac gtcaataatt ggaagatata tacagaaaat gtaaaatcac
     4141 agattgttga aaaaaaagag gctttaatta agaatggttt ttggaacaaa ataccatgga
     4201 attttcaaca aactattaat gaatcaatct tctgtttagg aacatttctt agtgattttc
     4261 agatagttga atcagctttt gaggaaaatt ttatttctga gagagagata aaacttctaa
     4321 ggaaaattgg taaaaattct gtgcaattta accatagtta cggaaaatac tttaaggaag
     4381 aatacgaatg gcgcgaatat ggaaatccag aatttgaaat cgttgaatca atttaccaac
     4441 atgggagaga tttttttgtg acgttacaag atgcaagtaa tgcagcagga cgactggagg
     4501 actatatgag caacaattca atatataata aagtaaatat atttggaaat agttacaaca
     4561 ctaatataca gcaaggccac aataataata tgattgtttc tattgatgat atgtcggaat
     4621 tattatcaaa gcttgatttg cttttggaga atttgaatga ttccttacct gaagaagagg
     4681 tagaagaggc tcaggagtat atcgaaacca ttaaagatga agctattaaa gaaaagccta
     4741 aaaaaactat gctgaggttt gcggtagata gtctaaaaaa acttaaatgg agtacagaat
     4801 tcttagcttc tgttgcaaca atagctcaac ttgttgctcc actgctataa gtggagaatg
     4861 aggtgttctt caattaaccg acttgccaag ttgtggtaat ggtttgagat aatgaaagct
     4921 ttgtattcta tagagattga tgtatctgaa tgaatgcttg tttttttgga tatagctgat
     4981 taatcagtgg tgttacatta tttataagtc gtttgattaa ctagttctta cgatttctta
     5041 tcaaaagcac gccacgcaac ttgaatacgt ttttgaatac gactaaaaaa ccacttaagg
     5101 tgaattagac ttaataaagt gattagttct attcaaaaat cgttgaaata ccaactatcg
     5161 tcaattatct ttaatgagct taacttcatg gcggaagaag aagaatagac tataaagcta
     5221 ggagaaacct tgatatgata gggtttctcc tttttttatt ttttactgta tgccatattg
     5281 tatgccatct agtcaatatt taagtgttgt actaattttt caacagtctt ttctttcgtt
     5341 tcttttgcca agtgaccgta acaatcgatc gtctgactaa tttttgtatg ccgtaatcta
     5401 gtttgaactt ctaccatagt agctccagcg tccaagagca ttgaagcatg agtgtgtctt
     5461 gctacatgca caccgagaaa tggaaccata gctttttcac aaatagtaat caatcttgca
     5521 taaatagcgt ggtgtgtgat tagttcttta tagataccac aaaaaatcat agtcggatca
     5581 gttacaccaa gtgtcagcat atatttccgc tgattaatct gccatttttt taatatagaa
     5641 attgttcttt catctagtgg aagaacccca gcactacaat cagttttagg atcactgatt
     5701 ttatagccat ttttggattg gcttagtgtt ttattgatgg tgaccgtatt ttcttgaaaa
     5761 tcaatgtctg accaattcaa tgcaagggct tcactgatcc taaccccgct ataaaagaga
     5821 aagcgtagta acgttttatc aaagtcgttt cgataagttt caggtttgct gtttaggtaa
     5881 tcaaacaagg tgatcagttg ttctttactg taaactttga ccttttcttt atgcttagtg
     5941 gtggttcttt taggcattaa tacgttgtct agtggattag tctcaataat cccaagattg
     6001 atagcgtagc taaaaatacg cttagaagtt gaatgaagta ccttgtattg gtcagtagtt
     6061 tcagcccagc tatttacaat ttgttgggct ttttttaccg tgacttcatt cacacgaaga
     6121 tttccatatt ttggaagaat ccactttcta aacttgattt cagttttcat gaaggtggct
     6181 tctcggacag tggttttata tgtttctagc cacaagtcat aaacttcttt aaacgtttcg
     6241 ttgttcgctt ttttaatacc gtttttatcg tagtccactt gcaagcgtga caaggcaagc
     6301 tgggcttctt tttttgtttt gtaacctcgt ctacgaacct tatcaggttt gccagtcatt
     6361 ttgttcattc caagataggc ttcatagagc caaaatttac cttttttagt ttcatattgc
     6421 ttgaatgttg ccataatagt tttccctcca tagtgtagca agccgtgggg gcggttggag
     6481 gaggcagttt attattattt tgaatagtgt ttatttagct cagtaacaag agctgtgaac
     6541 tcttttagaa gatcattttc ataaaattct tgatactctt ttttctcatc tgtatttttt
     6601 tctgaatcat taattaatga gtcatatcgt aaaagcgtat ttagaagagc tgcgagataa
     6661 gcaattgggg tactcccgtc aggaaattca attcgagatc ctgttttaag atattttatt
     6721 gcgtttgtta aataaatctt ctgtgagaca gtcaggttat cttcttcgat gttattgata
     6781 gcattagaaa gattagaatc aattatagag ttattttttt caatagtttt tttcaattgc
     6841 ttttcaagca catttcgctt aatttcgggc atcatatgca tatctcttaa tgtataagat
     6901 ccgtttaata gataggtagt ggatacatct aatacctttg aaagttgttt aagcctggtg
     6961 ttattgggaa gattatagcc attttcccaa ttactaacgg cacctttact ggccggtggg
     7021 gataatttac ctccaagttg ttccattgag agtccctttt caattctagc cagtctaatt
     7081 ctttgtcctg tctccactgc ttcggggtat ttttttctag ccacattttc accaccattc
     7141 atatattttc tctactatat cacgatttaa atatgtgtga aacataaaag ttcaaaaaag
     7201 tatgttaaaa ttcttgcaaa tgaaaaggta tgatggtata ttgagtttat aagaggtata
     7261 gaaatgtatt caagagcctc ttgcattttt ttaaaatctt tgtatagaaa tgtatgctaa
     7321 cgagggggga taaatatgga attaaaaatt gacaaactca cgcttgatga tccagctttt
     7381 aaaattattg acgatcgagt tgttcaacaa gtagagcaaa ctcaaggaac gttactttca
     7441 ttattagaaa aaaagtacca gtttccaaaa tatatgaaca aggcacaagc tgctaagtat
     7501 atgaacgttt catacaatac tatgatgcag aaatatgttc ctaacggttt aaagttgatt
     7561 atagttgatg gagtcgtaag aatttcacaa gatgaatgtg atcgctttat ggaagaatac
     7621 caaaaataaa cgcaagccgt gggggcaaag ggattaatag gaggaaataa taatggaatc
     7681 gacacaaaat aaagcaatcg aaaaagtatt atcaacaatt cttacagaag acacagcggc
     7741 agaattagca aacttggaag gcaaagcgct agaagaaacg tttgaatgtt tgtacgaaca
     7801 aatggactat caaaaattgc taccacaaga accaacggta agcggagtat tgcgtggctt
     7861 gaacgattta gtacaagcag agtttaaaga acgactttct attgaagagt atcaagaaat
     7921 tttgtatcag caagttgagt tgttagcgag cttgctaggg cttgaactgg aggacgttga
     7981 atgaaaactg aaatttggaa cggacacatt ataagatttg tcgatattaa tgatgaatgg
     8041 tgggcagtgg cgaaagatgt tgcggaagcg ttaggtctta aacaggttac tagagccatt
     8101 cactctttgc ctaaagatgg ggttactact agtaaggtca tcgattcatt gggcagaaca
     8161 caagatgtaa acatcatcaa cgaaaaaaaa tatttaccgc atggcattca aaagccgtaa
     8221 aaaagaagcc gaggcatttc aagactggat tttcgacatc atcaaagaac tacgccagtc
     8281 cacaggactt gaaggctttc aagtattccg catgctagac aaagaacacc aaaaagaagc
     8341 aatgactaaa ttaagccacg ctattactga accaaaacca gtagactaca ttaaagcgaa
     8401 tgtgatagcc aataaagctg tatcaactat ttatggtcat tccaaaatgg taaaaaagaa
     8461 agatatgact cctgaaatgt tggttgatcg tgaacctatt ttagatgaga cagtagaatt
     8521 aatgactgtt aaagaaaaat atgggttgca gttttcagtg agtgagaaaa tttataatcg
     8581 ttctgctgaa ttgcagacaa cttaggagca ttaacatgat cgagaacaaa gaaaaagcct
     8641 tatccataac agtcttggcg gacaggataa aggcaactta attgggataa aacccgatat
     8701 tctttgttcc gattatacca tattaaaaaa tcggagggaa ccattatgaa acgaaactta
     8761 attataggaa gcattttaat cggcggaatt ttcggcttat taccattaat tgcaaaaata
     8821 gctatctttg caggattagc gttatatctc ttcactgctt atgacgagta cgactatcaa
     8881 ttaagaactg gagggcataa gtagatgtac aaaacaaaac tattaaacca actcgatagc
     8941 cttgaattag aggaaattaa tcaagggatc gctgaactag aaaataatat cgggaaaacc
     9001 tactttggaa attcattcaa tgaaaaacta acagttttgt atgtactcaa aaaacatgca
     9061 gaacacaaaa taatttgtcg agaaatcaac gagctaaaaa atcagatttt aaccgcatgg
     9121 ttaaacatta ctgatatgca agaagcaagg gtgaaaacat ttaatacatg ggtaaagtat
     9181 cagaatcaat taaaaggtgc tgaatttgtt cgggatgggt tgaaatacga acttgagcag
     9241 ttgaagctca tggaggtgtc tgaatgattt ataccagtat tttgaaaaag gaggagaggt
     9301 ttattggcaa ataattatca agcagcaacg gaattacaaa agtcgggatg gtcaatttat
     9361 cccttatctc ctggaactaa aattccaata aaaggctcta gtggttggaa tgatgcaact
     9421 aacgattcta atcaaattaa tgaatggtgg acggaagatg caactagaaa tattgggtta
     9481 atgcttgaag atcatcaaat ggtggtagtt gacctagatc aacattctga aacacgaaac
     9541 ggcgttgaga attataaaaa acttgcttca atgtatgatc catttccttc tacttatacg
     9601 gaaatcacac caggtaaagg aatccatttc ttctttaaaa ttcctgatgg tgtaattatc
     9661 caacaagaaa caggtgcatt tgctgatatc ttcgggcgtg atgataaagg taaaagttta
     9721 agcgggattg atattatcac taaaggagtt ccaatagcgc caactatgat tatttcaaga
     9781 agtgttggga gagcatatga acctattgaa aacgtatctc taaataatat tgcaccagct
     9841 cccgattggc tagtgaacct actgctaaaa aaaccgcaag aaataaagac tcaataccga
     9901 cccgtagcaa aaacagggac agcaaaaaaa ttagatatga ttgttcaagg cgctggcgaa
     9961 ggtagtagaa acgatcattt aactaagtta tgcggttggt tactatggca cggtgtagat
    10021 aatcaaactt tagcagaatt gatttataca gcaaatgcgt acaatgaacc accgctaaaa
    10081 gataaagaag tgaatcaaat tattcgatcg atgattaaaa aagatttgag ggggaaagct
    10141 catggctcat aagcataaat tagatagtat tttagacttt cccgaagcca gcgagcgtga
    10201 agacaatatc attgaattga aaacctggat gtcacgatta agatgcaata aagacgatca
    10261 gattaaaagt aacagtgttg tgaatgcaga attgatttta accaatgata gcaatttagc
    10321 tggaacaatt gcttataacg aatttagcgg atacattcac ctattaaaag attctccttg
    10381 gatcaatcgt accgctggag aatgggaaga cagttttgag gatgccttaa cggcatatat
    10441 tgaagaaaac tacaatgtag tttttgatga caacaaaata cacaaagcag tagtaaatgt
    10501 ggctagaaag aatgttttca acccagtaaa agaacgtatc gaaaaagtta aatgggatca
    10561 acagcctaga ctggaaacac tgtttattga ccttttaggg gtagacgaca acctttacac
    10621 acgagaagta acaaaacgtt ggatagtcgg atcagtcgca cgtatctata aaccaggaat
    10681 aaagtttgag atcgttccag ttttggatgg accgcaaggg attggtaaga gtaccgttcc
    10741 agcattactt tacacggatg attttttcac cgattcatta gattcgctag gtgaaaagaa
    10801 agatgattat atgcagcttc aaggcaatgt aattattgaa cttggagaat tatcttcaat
    10861 gaacaaaact aaaattgaac agataaaaaa cttcatcagt gccaagatcg ataaaattag
    10921 acctccttat ggtcggaatg tcatagcttg ggcaaggcaa tgtgtattta ttggaacgtc
    10981 gaatgatggg cagtatttga aagatgatac aggaaaccgc cgtttttatc ctttgccttg
    11041 taagaataag ccaaagatga atccgtttaa aacaaaggat gaatattttc ttcaagtgtt
    11101 agctgaagca aaggtgcttt ttgataaagg acaacgaatc tattttagtc ccgatgaaga
    11161 taaagaagtc ttagaaattg caaaagacta tcaagaggat gccaagatgg agaatccaat
    11221 taaagaagca atcactaaat atttggaaat ggaaatcccg tataaatggg aagaagctcc
    11281 agcgtgggcg agacgttctt attatcagaa ctatcctgac agtaaatgta atgagaaaaa
    11341 tttacagcat ttctataatg gagctactat gcaacaagag ttttatttag ttgatagcgt
    11401 actcaccgct gatattttgg aagctgtttt tgataaacaa gcaaaggatt tattgaatgg
    11461 tcgatcagat gcagaaacta aaaagattgc cttgattatt agcagtattc ctggttggga
    11521 acggaagcaa ctgccaaaaa gaaataaacg acgaggattt tacaataagt caaatgccta
    11581 tgaaaataaa aatcgagctg gaaagtacaa aaagtaacgg gtagtaacgg gtcagtaaca
    11641 cctagcccgt tactagttta cccttactgc cccaagggat acagcaattt ggtaacgggg
    11701 taacacctta tttccttaaa acttttgaaa tagttagcta atatgctggg gaataatgtt
    11761 actggatttt acccgttacc tgttaccaaa acgctagaac ccttgtcccc gttgggattg
    11821 agtggtaacg ggtacctgtt acgtaggtgt taccacgtgt tacgaatcaa ggaggaaagt
    11881 aaatgctcaa tattatttca acaaacaaag caccgaactt tcaatataca gatgaaatgg
    11941 atcggtttct aatgaacacg ctggcattta gtgtgggatt ggtaacagaa gattattcca
    12001 catttgatcc agaagtatta aagataatgg aagaagaacc tgattggctg caggagtcag
    12061 tagcgtggtg tcaatcgcta gtagtgggat cattggtaga tagtggcaac tatgatgata
    12121 caggtgagct aatggatgaa tttaattgtc tgcttaacct atatgatcga gcgagacaac
    12181 gagagcttac atcgaatgag gataatttgt ttttaaacat ccatgataag ttcttggctt
    12241 tgctactaac agatgatgaa ttgataacta atttattgga ggtagaataa ttatgcatat
    12301 ggaaagtatt agatacttcg atggtgagaa acacattgag tattcaaaca ctcaagaaga
    12361 tgtagacttt attagttttg aggaagataa aatcactaaa gttaatttta aagacggaac
    12421 gtgcttgaag atagtttcgc cttatattga atacaaatca aaatgggaac aagatgattc
    12481 tgacgaacga gatcgctttg gttggtaatc tatggcagtt aaaaaacaat gtaatcatgc
    12541 tggatgtaaa acattgattg attataggca gaagtattgt gggaagcata aagcaaagca
    12601 aacggcaatt aagcgtgaag aaagaaagca aagcgaaggt aaatattttc aattctatca
    12661 aagtagaaca tggcgaaagg cttcatactt gtatcggtta aatcatccag tatgtgagga
    12721 ttgcctagaa gaaggcttga taaggaaagc tgatgttgta gatcacaaaa ttgaactaaa
    12781 agatgattgg aatagacggt tagatgaaac taattttcgt tctttatgcc atgcacacca
    12841 taattccaag acagccaatg aaagacaaag acgagaaaag agtacccttt gagttaaggg
    12901 ggcatagtgt gaaacttact gacaatcgat gcctactcat tttggtacaa ataaccgttg
    12961 tgaaaaggca taagggtaat tacaaataac gattacactt gtaatcacaa gtgaaaaagt
    13021 atataatgaa agtaggagat ttattgaaaa atacaattgt tattactgat aaaacaggga
    13081 aacagcgcac cattgagtta cctcattttg gtcaagtgac cattcaaatg cagaacggaa
    13141 aaatcattta tatagataaa ttagataaag aaaaattctg atcgaaaaac gaaggaatgt
    13201 gagcttaatt gcttacgttc ctttttcttt tgtctgaaag gaggacaaca tgggacaccc
    13261 taaaatatta cacgatacaa aaggcaatat atcaagtgaa gaaaaagcaa tccgtgtgga
    13321 cgctagagaa gaattgttca agcagcaacc attaataaat atcacgcccc ctgactggat
    13381 ggcagcgagc gctagaagtg aatggaatcg tatcgtacca acattaaaaa aagattatcc
    13441 attgagtgaa gcagattatg gttcattggt agcctattgt ttagcctttg ctcgaatgaa
    13501 aacagccgaa gccgagataa gaaaatcagg tacgtttatc acatgcgaaa acggagtaaa
    13561 gaaagccaat ccagcggttc gggttcaatc acaagctatg agtgatttga aaaaacaagc
    13621 cacctcacta ggtatgacct tagaatcacg atcaaaacta gctttgaaca aggttaaaaa
    13681 tgatgaaccc gaagacccgt tcaaagagtt gatgaattca tgaatgatta cattgagaaa
    13741 gtcctatcag gtaagctgat tgcacccaag aaaattatcc aagcgtgtga gcgtcatata
    13801 agcgatttgg agcgttctaa gtcagatagc ttcccttatg tgttcgatga agaacaagcc
    13861 accaaagcga ttaaattcat tgagttgcta ccatctacgg acggtaaagc aatcaagatg
    13921 ttaggatttc aaaaattcat tctaggcagt ctttatggct ggcgtactaa agaagggaat
    13981 tacaggcgat tcaatcgagc gtttaccagt atgagccgta agaatgggaa aacgtatatc
    14041 gcaagtggca tggctgccaa tgcgttgatt atggaacaag aaccagcaga agcaaggcaa
    14101 gtattgtttg taagtaacgc tttgaaacaa gctaaattgg gctatgatat gctgtctaat
    14161 tcacttagaa acgtggtcaa gtctagtaag tttttaagac cacaactgaa aattatgaac
    14221 tctaagattc agcacttgcc ctctaattcg ttcgctatgg cactggctag tgaaaccagc
    14281 acgctagatg ggtttgcacc aacaaccgca attcttgacg agtggcacga agcaaaaact
    14341 cgtaaaacgt acaacgtcat taagtcagga atgacccaac aaaagaacgg cttattgtgt
    14401 gttattagta ccgctgggct tgatttaaac gttcctatgt acgaggaata cttattgtta
    14461 gatcgtgtgc taaaaggcga agaacaagcc gacaggtact ttatagcgat atgggaattg
    14521 gacgatcccg aagaaattca cgatcaagag aaatggatca aagccaatcc gatttttgaa
    14581 agtgaagaaa tcaaaaaagt aatgattcca accattcaag atgatgtgaa ccttgctttg
    14641 aaacaaaata accttaattc tgtattggtg aaaaacttca atttatggag acaagcgagt
    14701 gaggacagct atatgattgc tgaagactgg caggcaaccg aagtagaacc acaagatatt
    14761 acaggcaagc ccgtttatat cggagtggat ttatctaaaa cagatgattt aactagcgtt
    14821 tcatggatcg taccgctaga taacggcgaa ctttattgtg attcacatag ctttgtagcc
    14881 accaaatatg ggcttcaaga caaagaaaag cgcgacggtt tgccttatcg agaactagaa
    14941 aaagctggtg agtgttccat tactcaatta gaaagtggaa tcgtggacta tgaccaagta
    15001 tttcagttta ttcaagattt gattcaagaa aatgatttgg aatgtatggg gatttgttat
    15061 gacccgtata acgctaattc gcttatcagt aaagctgaaa aagccaacta cccaatgtta
    15121 gaagtaagac aaggaacgat tactctaaac gttccgaccc gaacttttag agaacaagtt
    15181 tatgaaggca acgttattca caataaaaat acgattctca cccatgcagt gaacaacgct
    15241 attttaaaaa cggataacaa cggcattcag attaataaat caaagaacag taacaaaatt
    15301 gatccaatag ctgcattaat caatgcctat gtgtttgcaa tggattactt caccacaacg
    15361 gaaggagcga aagcagacaa tgaattttat acaagtgaag aattttcttt ctaattacat
    15421 tcataccgtt cttttacttc ttggattggt gtgtgtgttg gttgctatca ccttactaac
    15481 aaatgtctac tatggcttgt tagcgctggg catagtgctt attggggtag cggtcatgct
    15541 aaatacagaa cagaagggag gttaaaagat ggcatttttt aaagcgagac aaaatacaac
    15601 gggagatcct ttcctacaac atgtggtatc aatccaatcg gatgattaca ccaccagctt
    15661 tacaagcgtt cgtgcattaa gaaatagtga tgtgtttgca gccgttcgaa ttattgctag
    15721 tgatattgct tcaagtccga ttcaattggt caaaaacaat atgccacaag ctgataatga
    15781 actggtgaag ttactaaacg agaaacctaa ttcagaaatg gatggttggc atttaaagtt
    15841 tgctttggcg gtcaatatgt tgctaaacgg taatagcttt gcagagatta aacgcaacgg
    15901 tgaaaaggta gaagaaattc acttattacc taactcaagt gtaacggtta ctcaattaga
    15961 taatggtacg ttgtcttatc agattggtga taaaaaaaga cgtgtgaagt ctagcgatat
    16021 tttgcacttc aaatatttca cccaagatgg tttaacagga ttaccaccac tttatgcgtt
    16081 acgtgatgaa ctgaaaattc aacaagctgg caaccgcaca ttgcacaatt tctttactcg
    16141 tggtgtcagt gggtcaggta ttttgaaggt tcataagtca gatttagacg gatcagcaaa
    16201 gaacgcaatt cgtgaaaagt ttgaagaagc caacggttca agtagtggag ataatgctct
    16261 tagaacgatc attcttgatg aaacaatgga ctacaaaaca ttagaaataa acaccgatgt
    16321 tttgaagctt atcaattcca atgattggaa cacaaaacaa attgccaagg cgtttggtgt
    16381 accgattgag cgtttaggcg ttgaaaatga acattctagc accgttcaaa gtaatctcca
    16441 gtatatccaa agtacgctga ttcattactt taatgtgttt gtgagtgaat tagatacgaa
    16501 acttgaaacc aatatccgct ttaattccga tcagttacta gagactgatc ctgaaacaaa
    16561 agtaaaaaat gtattggatc aggtcaaagg gtcacttctc acgattaatg aggggcggtc
    16621 gaaaatgggg ctacccccca tggatggtgg cgatcgttta ctagcaagtt tgaactttac
    16681 gtatttagat acgttggaga aatatcaatt aaaagaacag gaaggagtta caccagttga
    16741 ataatgaaga agaaaaggaa aaacggctga cagaagaagc tgagctaaca gccgattctc
    16801 ccaaagtaga gaaagaaaat gaaaaacaac caacagacgg caaaactatt tcaggctatg
    16861 cgttgaaatt cgggcaaccg tcaaaagatt taggcggctt tgtagaagtg attacacccg
    16921 aagcattaaa agaggtggat ttatcaaatg tgttcttatt atatggacac gattattcaa
    16981 agccactagc tagtgtcaaa gcaggtacgt tgaaattgaa tgtcgatgat acaggattac
    17041 attttgaagc agagctaacc gacacgacct attcaaatga tgtgtatgag aatatctcaa
    17101 aaggggttgt tgattctatg tcctttgggt tcgtgcttgg attagattca ttcgacaaaa
    17161 aagaagatgg cacaattgaa cgatcaatag ataaaatcaa agcacttaat gaaattagtg
    17221 tcgtgaccgt tcccgcttat gattcatcaa atgtccaagt aaataagcgt tcttacgaat
    17281 cgtttatgag taacaaccaa gcaaaacaaa caaacaatag cttagaatcc acttctaaag
    17341 cacaaaagga gagtaataac atggaaaaaa ctttaatcga taacgaaaaa acagaattgc
    17401 gtgggtatga agaatatatc cgttcacaag gcgaagtgcg tgatggagtc actactgtaa
    17461 atgcagcggc agttgttccc gaagaagtaa tcggtgaagt ctttgatttg aaacgttcaa
    17521 attataactt agctcaatat gcaacagtaa aaacagtatc aaatggacaa ggtaaatatc
    17581 cagtagcaac taaccaacaa gcagtgttag caacaaaagc tgaacttgct gaaattggtg
    17641 atattgacgc tgaaatgttt acttcagttg attataaagt agaaactcgt gctggtaaga
    17701 ttgccttatc aaatgaggtt gtggaagatt cagcagtgaa tattgtacaa gaggtcaaag
    17761 atcagttagc gaaattggta gaaaacactg acaataagca tatcatggat ttattaaaaa
    17821 cattcactaa gaaaacggct gctacgttgg acgatttgaa acaactatac aatgtggcat
    17881 tagacccagc attaaataaa atggtaattc taaaccaaag cggatattac cacctagata
    17941 cattgaaaga ttcagatgga cgttacattt tacaacccga tgtgacagca cctagtggta
    18001 aatcattatt cggtatgcca gtagtattga ttgcagatac attgtttgcc aatcctaaag
    18061 ctggaacatt ccctatgatt atgggggata ttgcacaatc tatctttgtt gctcgtagaa
    18121 atcaagtaac gactcaatgg gaaaaattcg attactactc acaaggactt gcagtgatcg
    18181 ttcgcaacga ttataagaaa attgatcaaa atgcttcagt gtatattgag tttacgccag
    18241 ttgtaacacc aaaagtatag aaaaatattg ggcggcggtt tatcccactg cctttttttc
    18301 attaggagag ataaaatggt aattttggat agcataaaaa aaagtatgcg gatcgatcac
    18361 actattgatg atgactttat tcaacaattg attgatacag caggcgaata tataaaaagt
    18421 gctattgata gtagcgcaac ggataaagat atggataatt atcagcaatt tgatttggcg
    18481 gtgtcattac ttactcaaca ttggtatttg aaccgtcaag aagccagcag cgaacggata
    18541 ccagtaacgg tacaagcatt agtacaacaa atgagaggtg cttattatgc caatcattaa
    18601 gaatgtaaat gaattgactg agagagtcaa gtttaaaaag acaaaacggg taaaagatga
    18661 agatgggcag atggtggata gtgaagaaac tgtatttgaa tgttgggcta atgtgcgttc
    18721 acaaatgcta aaagacgtgc ttgctagtgt aggtaccatt cttgaaggaa cgttgacgtt
    18781 tattattcga tacgatcaag attatgaact aacaaacgat atgaaagtag cttggaaaaa
    18841 caagagttat aaaattattt caatcaatga aggaacggcg tttaaagatt atacaacgat
    18901 catagctaaa cagatttctt agaatgatta cacttgtaaa cgttgtgggt atttagtata
    18961 attaaggtag taaatgaagg gttagctgcc tgaattttgc aagacttagc tagtcgaaat
    19021 ctattgttgg actgaaaatt gtaggtgtgg ttgcaaacat gctggactaa gaaagatgat
    19081 agatgatgaa cgttcaattt taatacaggc atgctaggac tgattggatg gttttggcaa
    19141 gctgattgtg tagaaatcaa agaacacgtc tgttgtggac gtgttttttt tgttataatt
    19201 caaaagaggt gatttaatgg acaaagcaga gtttacaaat ttttattgga agtattatct
    19261 acatttagaa aatgagttta ttaataccac agattttgtg atgctcgatg agaataattt
    19321 caaaacattc tctattgaat atcaaaaact actactagcg attggttcgg agtgtgagat
    19381 tattttcaaa gaactttgcg gatttgatag cagtagcaat aaaagcataa ctgattttaa
    19441 agctgttatt aaatctagcg aattaatgtg tttggataat ggtgttaggg ttatgaattc
    19501 tttaacattg acttcactaa aaccttttgg agatgggtgg cctgaagata caccagattg
    19561 gtggaaaata tataacagag taaaacatgg tcgttcttca aattataagg aagcaaattt
    19621 ggaaaatgtg ttgtatgctt tagcaagtct atatttatta gaggaatatt tgcataagaa
    19681 agtaatacta gaaggtgaag tagactttat aagtccagag tcaaatctat ttacgttatc
    19741 ttgggaaaga aaacattcgt cgatgcaaaa agtgacgttt gaaaaagtgg attcaaatta
    19801 tgtagctcca ctcattgatt ttaaagaaca agaagaaatt taaaaacccc cacggcttat
    19861 tgaagttttt gtatgccata ttgtatgcca tttggtttaa atcgcttgaa aaatagtgat
    19921 agataactct aattcattta gtcataaacg ctgttttgat aacctttata gttctttgat
    19981 atgggtttct cattcatggc ggaagaagaa gaatagaaca aaagaatgaa ttaataagat
    20041 ataaaaaaag ggattgtgac aaacgtcttg tcacaatccc tttttccaaa taaacgatgt
    20101 tcaatcagct gcccctcggc attcgctcga aaaacggcga aatatgaaga actattttgc
    20161 catttgtttc tctaataatc aggtcaaaac gttgacttca cagtctcttc cttaaacggt
    20221 gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt
    20281 tttgaaattg ttccttgtga ctcggagctg aacggcaggc tcacgacctc tttcttaaac
    20341 ggtgttcaaa agcttgcatc tgcggtaata agttcaacca aacgaaaatt atgaggagct
    20401 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctcttttcg
    20461 tttagcacta aatttcctta aaaagtagaa aagctttcga agcctcgttt gtttctttta
    20521 ctgtataata aaagaaaaga gtaacaatcg ctaaaagata taaacaagag aaaggaaata
    20581 caagatgatt ggtctgattt attttgtggt gatcgttctg gcgaacacag ttggcgctat
    20641 ttccgggatg ggtggaggcg tgttgatcaa accgattctt gatcttatcg gcgcccattc
    20701 tgttgcaggg atttcttttt actcaacagt tgcggtattc acgatgtcca ttgtttcgac
    20761 tgtgcgacaa gtatcaagcg ggaaatcact gaattggcag attgtcggtt gggtatctag
    20821 tggagctgtt gttggaggag tagtcggcaa cattgtattc gaagtttttt tacagttatt
    20881 tgaaaacgag aagcatgtgc agctgatcca aatttttttg acggtattga ccttggtttt
    20941 tgcgttcttc tacaccaagc atcatcagcc aaaatttcat ttgacgtcat ggacttggta
    21001 ccttatatgt gggggggttc tgggcttttt agctagtttt ttaggtattg gtggtggacc
    21061 agtcaatgtt tctttgttga tgctgatgtt tgcattgcca ataaaagaag caacactata
    21121 ttctctatct acgattttct tttctcaact ggcaaaattg gtaacgattg ctttaacaag
    21181 tagctttatg cgctttgatt tatctatgtt gttttatgtt atccccgctg ctgttattgg
    21241 aggtttgtgg ggagcaagat ttagcagggt tttgtctcca aaaaaagtga cctttatttt
    21301 tcaagtaatt gtcctcgtgg ttcttttggt caattgttac aatgcctttg tcatcttaca
    21361 aacattagga gaatctagac taacgcaagt caaaatcact gaaacatatt gttctaaatg
    21421 aaaaaaagag tatactatga taaaaaaatt cattttaaaa aatcagaaat tggctggtga
    21481 tttggatgtt acgggaaatg cgtttagaag atgcagaagc actacgtgaa atcaatgcgg
    21541 atcaattagg gtatgatata ccattaaccg ttacggtgag acaaatgaag aaactgctga
    21601 aggagccaga gaaaaatttt ttcttggttt atgaagatcc tacttctaaa aaagcagctg
    21661 gctatgtaca agcggagtcc tatgaatcga tttttagcga tccaatgttc aatattatgg
    21721 cacttgctgt gtctaaatat gctgaaaaaa gaggcatcgg aaaggctttg atgactggcg
    21781 tagaagaaga agcgaaaaaa cgaggaataa cgacaataag gcttaattct gcagaataca
    21841 gagtagaagc acaccaattt tatgaacaca tcggttatca ttccgataaa atgcaaaaaa
    21901 gatttttgaa aatcctgtaa atctttatca aaaaaatacc cgatcaccaa gccgatactt
    21961 gatgatcagg tatttttgtt tttttgacta agaaagtaat tgcatcaaga aaccagagat
    22021 gatcaaaacg atggcaccac caagtcgatt gcccatttga gcaaaagcaa taagttccat
    22081 ccgattagct gcagataaga cggctacatt tcctgttccg cccatcgagt tgttgcataa
    22141 gccagcagta atagttgatt caattggata taaaccaaac aaacgaccga caaatcctga
    22201 aacaactgaa atcgcgataa cacttgtcag acataagaca acaaattgcc atgtcaaagc
    22261 agaagctaat acattcagat tcagcaacgc aataccgata ccggcaagaa cagctgctgt
    22321 caagtttttg acaatcaagt tattgaacat gatagctgca tcttcataat actctggtaa
    22381 taggttggcg attttacaga tgacaacgat aataatcata aatgcgtaag catggacttt
    22441 aggaacaaaa tagttaccaa tttgtcctaa gatgaagaaa gtcaatgata ccatcaaacc
    22501 aacacctaac tgtggaatgg aaggtttcac agttcgaggt tttccatcgc caagatcgcc
    22561 tttttccatt aatttaccat tgccgttgaa ttttggcatt gattgtccag cacgagcaac
    22621 taatgctgca ccgatgatag ctaagacatt tgtcattgct gtcgcaggaa tcaggcggga
    22681 aatgatagaa gaagcatccg tacctaaaac atttgcataa atcgttgata aaggaacggc
    22741 tccggctcca acaccaccag ccatcgcagg taatgagatg tataaaacgg aatttgcaaa
    22801 accatttccg ataagcatgc ccacgagtcc tacggcaaaa aaggagacga ccattgaaat
    22861 aagtgcaacg ggaataaaac gaacagaagc tttcatcaat agattacggt tcataccaag
    22921 gatgctacca gtaatcaagg cagcaatata aaagtcccaa aagcccatat tgctgacaaa
    22981 gttttctgtg gatttcacta catattcagg taagatccca aatgtagcaa tcgcagcaga
    23041 agcgaagatc gcaaaaacgg aaccaccacc caaataactt ttgacgatag ggatttttgt
    23101 cccgataaaa tgaaataaat taccgaaaat aaccaaaact gcgattggtc caatcatatt
    23161 cgtcggtaat ttccctaaag ccattgcaat gatcaggaca aggaccatga tcaaataaag
    23221 tggcaaactt acgccggtga tttttgtgga ccaaaaacct tcttttttct tttcctcaac
    23281 tgcagttgca gttgttgttt tttgtgccat ttttacagat cctttcttat tctgtatcat
    23341 atgaaaaaag ccaaagaaaa gcgacaaaag agcaggagtt ggctggccgc tgttttgccg
    23401 cagtcgcttt tttcttttta gacgtcatac aaagtttctg tatgttcttt tactataacg
    23461 atcttaatct tctaaagatt tatattcagg aacccattta gcagattcaa ctgcttcttt
    23521 gatatccgtg atttcttcac gattcaagtt ttgtgcgaca actgattttg cgacagtttc
    23581 agcaatcgtt tgagaaaatt cagtgatttt tgctactgga ggcaagatag ctgctcctgg
    23641 ttttgttaca tcaacgattc cgcctaatgc tcgactagct tgagaaatga tttcagcatt
    23701 tactcgtgta gctgttgaag caatcaaacc aagtcctaaa cctgggtaca tcaaagcatt
    23761 atttgcttga ccgatttggt aagttacacc attatattca acatcagcag cagggatacc
    23821 tgttccaact aatgcttttc cgtccgtcca ttctattaaa tcttttgctt ttgcttctgc
    23881 caattttgtt ggattagaca aaggcatgat gatcggacgt ggtgtatgag cagccatttc
    23941 tttcacgatt tcttcagtga atgctccagg ttgggtagaa gtaccaatca tgattgtcgg
    24001 atggatcgct tttacaactg cttctaagtt tgtcaattca tcagcattag tgaaatctga
    24061 gcgtttgcga gcgaatggga tttgaccagg agtcaatcct tctgtatctt caaacaataa
    24121 accttgttta tctacttgat agaaatgttg gcgtgcttct tcttcaggta caccttgacg
    24181 gatcatctca tctaaaagga tgtttgcgat acccacacca gcagtaccag caccaaatgt
    24241 caaaattatt tgatctttca attgttcacc ggatatattc aatccgccta atacacctgc
    24301 taggacaaca attcctgtac cttggatatc gtcgttgaaa gtagtgattt gatcttcgta
    24361 tttttctaag atcgctgcag catttcctcg tccaaaatct tcccaatgca ataacaattc
    24421 tgggaataat tcggtagctg attgaacgaa ttgatcgatg aattcatagt aagtttcgcc
    24481 ttctacacga gcatgacgat tgcctaagta taagggatta ttcaataatt cttcattatt
    24541 agtaccagcg tcgatcgata caggcaaaac ttgtgctgga ttaataccag cagcagcagt
    24601 atagaccatc aatttgccga tagcgatgtc aacaccgttc acaccccagt cgcccatacc
    24661 taagattcct tctgcatcag tcacaacaat caaacgaatg tctcgtccat ctgccgcgtt
    24721 cttcaaactt gctttgatgt tttctggttc gtcgattgaa agaaatgcag catcttgcgg
    24781 tttcaagaaa atttcgttgt attgttcgat tgaatccgca acgacaggat cataaacgac
    24841 aggcatgaat tcaactaaat gttcgcccat caatttataa aacaatgtcc gattcgtatt
    24901 gaatagattc attaagaaga tacgtttttc caaatctgtt tgtttgctta agtattgacc
    24961 ataagcttga acagattgtt gttccagtgt ttggacagta cttggaagca taccagtgat
    25021 tccatatttt gcccgttctt cttttgtaaa agctgttcct ttatttagaa aaggattatt
    25081 taataagttt aaccctgtaa gcatgaatga ttcctcctaa gttttaatac aaacgaagta
    25141 tagaagtcag atgatgatat agtcaaaaag agcaggtatg ataaatttta tttatatgta
    25201 atatagccaa aagaaatcaa aatagcagta tttgttcttt ttatatcgac aatccttgct
    25261 tgaatactat gcgtatctct tatatgctag agaaagaaaa gtagaagaat aagggataga
    25321 tggaatggat aaataggatt tctgaggaaa aaagcacatg atagcgttga taggagtcac
    25381 ctatgtcagg tgataaaaat acataagtag aaaattaaga ggaggaagag aaaatgaaag
    25441 aagttcaatt gattagaaaa agcgaactat cagaaggcgg ttgcaatgca tgtggcgtag
    25501 tagaagcgac aagctataca ttaaaattag attccaatca agcaataatc tctgaactga
    25561 cagtaggcgg tcttgtggac agtctggcgc ttgctgaagg ttttactgga gaagatattt
    25621 atgagatgtt ttcagaagtc cgtcagttaa aaaaaggaga aaaatgcata gaggttcatc
    25681 atgagagccc gaatgtgagg ttcaaacgtg gtgataatga gatgatcttc aagaatcatg
    25741 tgtctgatca cactgaacta tatgaaatcg tgaatcaaat actgtctggg atttttgaat
    25801 taggacctta tgagttcaag gaagaaaacg gtaatccaaa attaaatgaa gaatggcaag
    25861 aaacgattga aacacaaaga aacaatccac acttatttca atagtcttcc acagtatttc
    25921 gtaaaaatgt tgatgaaaaa gaaacaccgt gaatcagagg tcgtgaaaaa cctgccctgc
    25981 atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct
    26041 tattaccgca gatgcaagtt tttgaacacc gtttaaggaa gagactgtga agtcaacgtt
    26101 ctgacctgag tattagagag acaaatggca aaatagttcc tcatattttg ccattttttg
    26161 agctaatacc gagggtcagc tgattgaaca ccgttttatc gagaggttgt gaaaaagtcg
    26221 cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
    26281 ttgacttaat accgagaaaa tggcttttga acaccgttta ttcaaaaaaa gggactttga
    26341 cactactttt gtcaaagtcc catcggtttt ttatctattt ttattacaat catttcttcc
    26401 agagtagtga catctggaat ggctgaaatg taaacagatt atttgcctgt atcaatgtcg
    26461 aagagtaata taaaaagtgt aaacgctatc atattgttca tcattcaaat tttatagaga
    26521 gaaggcattg agatggaaaa aacatatcaa gcaggtacaa atgaaggaat agtagatttt
    26581 atcaatatgg aagatttaga aatcgcggct tcacaagtga ttccagctgg tggttatggt
    26641 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
    26701 catcgtttga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
    26761 tttgacgaag aaatgctgac tgcaccaatc atcatggcac cagttgctgc tcatggatta
    26821 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
    26881 acagcaagtt cttacgcttc ttgtacttta gaagagataa gagaagcagg cggggagaaa
    26941 gcaccgcaat ggtttcaatt ttatatgagc aaagataatg gaatcaattt ggatattctt
    27001 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
    27061 ggaaaccgtg aaacagaccg cagaaacggg tttaccttcc cgctgccaat gccgatcgtt
    27121 caggcttatc aatcaggagt aggccaaacg atggatgctg tatataaatc ttccaaacaa
    27181 aaacttagtc caaaagatgt agaatttatc gctgctcatt ctgatttgcc agtctacgtg
    27241 aaaggtgttc aatcagaaga agatgtctat cgatcattag aatctggtgc tggtggaatt
    27301 tgggtatcta accatggtgg ccgtcaatta gatggggggc ctgctgcgtt tgattcgttg
    27361 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
    27421 cgtggacaac atgttttcaa agcaatcgca tcaggtgccg atcttgttgc tattggcaga
    27481 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
    27541 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
    27601 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa taaatgacga aaaaaatagg
    27661 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
    27721 atttgaaacg aaaaaataaa attgaaatat tttttcatac ttaataaaaa aggaagaata
    27781 ttcatgtaaa cgattttatt gtttttttct cttttataca aaaagggtca taatttcaag
    27841 taagcggatg aattcatgag gattttttgc atactgtctc tactatactt aggtcactaa
    27901 gaaaaaggag tgatttatta tgaaaaagaa catgattggg acattattac tttcaacact
    27961 tcttctaggc ggcttggcaa caccagcttt tgctgagggt caagcgactt caaaaggaga
    28021 tattaccttt actgaaccaa caaataccgt tgagcctctt aaccctactg atccttcaaa
    28081 accagtagag ccagcagatc cggaaaatcc agcaactggc cagacaggtt ctttaacatt
    28141 ggatgtagtt cctgaattgc catttggtac acacgagatt gaaagcggaa caaaaacgta
    28201 tcaagtagac gcttcaaaaa acgatacacc ttatctccaa gtatcagatc gtcgaggtgt
    28261 aggtgcagat ggccaagcac aagggtggaa cgtgacagtt tcggtttcag attttgtaaa
    28321 cggttcacaa gttttgcaag gcgctgaatt agattttgga acatccactg ttaaaagtac
    28381 gtcagataac gaatccactg gaccaactag tcaaactgta actgggttga gtaaagcaag
    28441 tgcagcgaca ccaatattta cagcagcaaa agatcaaggt ctgggaacat ggctttctgt
    28501 ttatgatcca gcaaatatca cactgaaagt gccgaaagca gcagctggaa cattcacggc
    28561 tgatttgact tggaacctcg tggctggccc agtagcatag agtatgaaat aaattcaaca
    28621 aactgcggtg aaaatactat aggtgaagga ttttttaccg cagttgttgt tgaaaggatg
    28681 tggtggacaa tgaatcaaag taaacgactg tttgtttcgt ttttttctat tttaagtcta
    28741 tttttcattt ttccgtcaat cagccaagca aaggattcag caggagactt tggaatcaaa
    28801 ccggtatttc cagagaacca aatagataaa gcaatcggtt attttgattt attagtggca
    28861 ccggaacaaa atcagacact agaagtaatt atcagcaatt cttcagatga agaacgaaca
    28921 tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga ttatagtcaa
    28981 aaaaacccaa cgttagatga gacattgccg tttgatgtcc gtgacgtttt gctcattgcg
    29041 aaaaaggaaa tcaatgtatc agcacatgca gaaacaacta tacctataga agtgaaaatc
    29101 ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc atgtttcacc taaagaagaa
    29161 gctgaaacag aaaatgctac agaaggagca caaatcaaaa atcgtattgc ttataatctc
    29221 gcagtcgttt tacaagaaag tcaagagacg atagaaccgg atctgaaact tttatcagga
    29281 gatttggacg aggtcaacgc aaagccaact gttcagcttc gtttccaaaa tcctcagcct
    29341 aggattatca gcaatttgat atttacgagc aagatctttt atgaaaatca gctttatatt
    29401 gaaaatacgt ctaatgaatt tttagttgca ccaaattcga attttcactt gaatcttgat
    29461 ttagctggtg ataaagcaaa agccggcgat taccgcgcag aaatcatagc aaaatcaggc
    29521 gacagtaatg aatggcgatt tacacaaaac tttacgatca aaaaagaaaa agcacaaaaa
    29581 gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt tcccatggct ttatgcagga
    29641 ttggttgctg cagttctagt gttatggttg attttgctgt ttttgtgggt ccacaagaaa
    29701 aagaaaaaag gagaaacaac atgatgaggt gatttcgttg aaaacatcga tcttattggg
    29761 agggattatt gcaagtttgc tgttcttcag ctggcaagag ccagtccaag cagataccaa
    29821 aagttcaggt acgattacat tttatgatct aagtccagtt ccgcatccaa gtggttcagt
    29881 caatggcgca tcaaaacagc agacacagct accgatttat aaagaatcta atcaacatgg
    29941 cgtattcccg gagacgaatg aagaaaagat ccgttttctt tatttgatca ttggtataga
    30001 attatggctt cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg atgaacatgt
    30061 tgctacaaaa taaattttca ttttttcgct ccttcttttt tacttcgctt tttgttttaa
    30121 gtatatttat cttttcacct tcggcagaag ctgttggagc aacgcctcct gccatttctt
    30181 atgatccata cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt gcagtcaatg
    30241 gcgggaagct tgtccaacta actggggcta accaattgcc taaattgact gctacttatt
    30301 tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt ccgttttcat tgggaaagcc
    30361 atgtgaaact aacaaatgta cttgatttgg tgaatatcac tgcacaagga tttgcatttg
    30421 tggcgatcat caatttgccg gaacatgtat caagcagtga tgtattaaaa gcaattgact
    30481 ggaacaacgc ctacttgtat attgccggtg cccccatcaa ggttactagc ggtagtttat
    30541 tatcagcagg aagccatacc ttgcgttttg ggatggggtc gtgggataca aatactctat
    30601 tggcagatat tattgctatt attacgcgag gcgaattcaa cgtggacgat atcccgcttc
    30661 aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa cggagataaa tatgatacga
    30721 gtccaaacaa aattttgacc aagggtaaat tacaaccttc tatgtctaaa aaggccgttt
    30781 tttctgttga tttttatgat tcgaataata ttatccaaga tcgtatgttc aattcgattt
    30841 tagctccagc tgcaggtaga ttttatccta aattaactgg cggaaacttg aatccaggag
    30901 catatgcatt ttctgcaagt gcagagataa atacgtggaa tagttacatc tcaacaagtg
    30961 attcaacagg aaaatataat acatttgctg ataccacaga atacgtggat ggaaataacc
    31021 agaaccttcc aggctctacc aatagtcgga cattgacgat ggggctgact gaaggacgtt
    31081 ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa ctattttaca ggaaaaaacg
    31141 ttacacaagg agcaatactg actcatactc cagcaagcac aagtggattg aatcagacag
    31201 ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt gctttctcct gttgctttaa
    31261 aggtattgga gcagtacaag ctagacggaa cggttttacc aacaaactta acagccgata
    31321 gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt cacggccaat tggtcagcaa
    31381 ctgaactaaa aaagggttcg atccattaca ggctttattc gaaaggaacc caaaaattag
    31441 cgacaggatg gacagatcaa cttttccaaa cgatccaatc aaataacggg ggtgtaaata
    31501 cagctacttc tacactacct gcattggctt cagggcaata ttattttgat tatcgcttgg
    31561 tggacgatgt acttactaac atgtatccgt cttttgcgta caaatggcag tctgaacaag
    31621 ctggaatcac cactcttcct caaataacag tggctaattt tccatctgtg agtgcagata
    31681 gtcagctaaa aaaccttagc agacaacctg agacaggaga tcccctgtct gctctatcag
    31741 gagatacgat tcaagaaaat gtgacattca cgttaacaaa atctggggat gcgataaccg
    31801 acaaaaaaat cacgatctca cttcctgaaa atactactta tatcaatggt tccttaaaat
    31861 taaatggtac aactatcgca gataccggca ttcaacaagg agtttctgtc ccagcagatc
    31921 tattgagcaa aataggagat accattcatc ttacttacaa ctatcaatta aatactgtag
    31981 atacttctgt acaatccgtc agcatactga caaaggcagc tgtactttcc agcaatatta
    32041 cattagcaga tggtgctaag cttcctaacc cagttgtcca aacatctgct aaaacaattc
    32101 ttgtccccaa acaagaatta acattagtta atgtaccaga tgattttaca tttggtaacg
    32161 atctccccaa accgttgaag acaagttatt atgaagcaaa aggcgatttt tcttttgatg
    32221 tgcgggatac tcggcttcca agcacgtctc cttggcaact gacagggaca ttgacttctt
    32281 tatttaagaa tgatcaaggt caagaattaa gcggaacaaa attatacttc aatcattctg
    32341 gatcaaaaca attgattcag caaggtcaaa acacgttgat ttatgagagt gatggaacag
    32401 ctaaaggaga ggttctcgtt gactttcccg ataccgatgg tttactctta gaggtaaata
    32461 gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg gaactgacag
    32521 ctgggccaac cagctaagtg attagaattt gtatggatct gtgataactg tcacagatcc
    32581 tctttttcaa ggatgagaag gaaacttctg ctgttaaaat cagaaatgct tggaggagtg
    32641 gacttggaac ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa acttttccaa
    32701 cttttattag aggaaaaagg agagatttcc gttaataacg tacttacccg cttatcagtt
    32761 gatcgggcta ccttgaaaga agatctgttt tatttacaag ctaacttgga aaatatcaaa
    32821 caattggaaa ttgttatcca agaagggaat attttattaa tcagacatgg aaatatagca
    32881 gcagtcgatg tttattatca ttgctacttg cgctattcta caaaatatca gatacttctt
    32941 tatctgttgg aaaatggctc atttgatcgg cagaaacttg tcatggaact taatatcagt
    33001 tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga gtttcatctg
    33061 aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa tttgttattt ttatttccaa
    33121 ttattttggt ttggacgtcc ttatcttgaa aacaaaaaag aatacagtga tttatcagga
    33181 aacacttttt tagatctgct gcaaaaagag tttagtttta ctattacaga agaaggacga
    33241 ttgaaatttg aaatatggct ttccattatg aagaagcgaa tgcgctgtac gaaaaaatcc
    33301 aaaccagatt atagcaatct tcctgttgga ttgtttcaaa cgacacaatt tttattggac
    33361 ctccatacat ttttgacccg ttacttcttt caatatgcct ttccttggaa tcaaaatgaa
    33421 acgttggttt tttatctttt catgatcagt aactttacat tagatacaca taactcgcat
    33481 gtccaggagt ttttgataag cgaaaaatac aaagacgacg tagtagctca attgaatcga
    33541 acgtttaaac aatcgataga agaatatttc tctttttatg tttttccaga agagatttcg
    33601 caaaagattg cgttgacagt gatgcaaaat cacgctcatt ttgcctattt tagaggatgg
    33661 atgaatattt ttggccaaga aggattaaaa cagcggttaa ctgaagtaga agataaacaa
    33721 ttcctggttt tatgtgaaag attggtcaga caaacatttg ctattgttga ttttactgaa
    33781 gtagacgagg aaaatgcccg ctttgaatta tttgctcgct atgcatctat tttgcggacg
    33841 ctgatcaagc agttagaatt acgtctgcaa attgcttatg atcttcctta tgaaaggcta
    33901 atggcggata tcgtgaaaga aatcatccag gaacaattgc tgaatacgat caaatatgat
    33961 ttagtcgaat acgagaaaga aaagcagtat gatatcattt tgactagcca attgaaagag
    34021 tacccgtcac agaagtccgc taaagttttt gtattcacta gcaacgaata taattatgat
    34081 ttcccctatt tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa tttaaggatg
    34141 aataaaacat gaaaatcgtg atcattggtg cttcccatgc aggcatcaca gctgccctaa
    34201 atctaagaaa acttcaaccg aaagcagaag ttttactgat cgatgaagat cataaagatg
    34261 gactaggcta tgtttcaaat ggaatcaacc tttatttaaa aggaaagata cgtagtttag
    34321 ctgaagttgc ccataacatg cggactcttc aagaaagtgg ggcaaaactg atcaccgaat
    34381 ggagagtaac tgaacttgat ccagacaaac atcagctcat cctcgcatcc aaagaaggaa
    34441 aaaacgagac cataacctat gataaattaa tcgtagcaac aggttcctct cctgtcacct
    34501 tgtataaaca aattgaagct gaaaatgtct acacctataa aaatctagtg caatctaagc
    34561 aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct ggttatattg
    34621 gattggaatt agcagatgct ttgagaaata aaggatacat tattcatcta gttgattata
    34681 tgccgaatgt tttatcaaga tatttcgata aggatatgat caacagtttt caaaatcagc
    34741 tgcaaactaa gcaaatcaat ttttatccaa atgaattctt gatcgattgg aaaaaatcag
    34801 aagagaaagt agtatccgta caacttctat ctcaagcaat aaaggcagat atggtcattt
    34861 tttctgccca aactcgtcct aatacgacat tgttaaaaga aaaagtggct ttgtatgagg
    34921 atgagactgt gatagtcaat gaatatctgc agacaagtga tccggacatc tatgcgattg
    34981 gagatatcgt cccagtcagt tttgacaaaa ataaacggca tctcttttta cctttagtta
    35041 ctcgagctgt acatatggca agagcagtag cgctaacttt gagtggtcag ccaaccgctt
    35101 acgacctcag acaaaagata acagcagcag tcattataga ctacttttta ggaactgtag
    35161 ggctgacaga agatgaagcg ccattcttgg aacaaaatac cggttcgtgt tcaggagaat
    35221 ttgatctatt tccacaatac gatgaagaaa acaaaacagt aaatgcaaag ctgatctacc
    35281 atccggatac attagaaata attggcggac agctgataag ccaagaattc ttattatcag
    35341 atttgaacct gcttgcggat atcgtgaaac acaaaactac gattccacag ctagcagttg
    35401 aggattttgg atttctgtct gaacacacct ctcgttttca ctacctcaat gagttagctt
    35461 ttaaagtact agctaagaga gcaaatagca atagagtaga taaaagactg tgagaaatat
    35521 cacggtcttt tattgtgtgg ggatataata aatattaatc aatcgagaat aaaaaattca
    35581 tataatgaaa tagcggaaga attttttgag cataatattc gccaaaaatt tatcaagtga
    35641 taaaattgtt gtataaaatg attatttcag gaggaggagt taaatgtttt tagcattaaa
    35701 tgaaattatg cattcaaagt tacgttatgc gttagttgca ggggtgatgt ttttgatcgc
    35761 ctacctggta ttttttttga ctgggttagc ttatggctta gctcaagata atcgaacagc
    35821 agtcgacaag tgggaggcag attcgattgt gctttcgaaa gatgcaaatt caaatctagg
    35881 gatgtccatg attactaaga agatagcaga agaagtcgaa ggtgggaaag tggcgtatct
    35941 tgcccagaca cctggagtcg tgactagtaa agacagtaca gaagaaggaa aaatcaatgt
    36001 tagcttcttc ggtatcgata aaaatcaatt catcatgccc aatcttgttg aaggaaaagc
    36061 ttttgataac gatgatgagg cagtaggcga tattagtttg aaagaagaat atggtctagc
    36121 cgtaggagat acagttaagt tatctggtag tgacaagacg tttaaactaa ctggctttac
    36181 ggatcatgcc aaattcaatg tctcaccagt tctctataca accatcaatg cttaccaaga
    36241 gatccgcttc gaaaaagaag atacaagtga aaatgcacgg attaacgcaa tagttgtcag
    36301 aggtaaaatc agtaatttac ctgaagattt agaacagatc aaaatttcta aattcatcaa
    36361 tgaattacct ggatataatg cgcaagtatt gacttttgga tttatgatcg gatttttgat
    36421 cgttattgcg gctattgtga tcgggatttt catttatgta ctgacgatgc aaaagattaa
    36481 tatctttggt gtaatgaaag cacaagggat cacaggagga tttattgcgc gttctgtagt
    36541 ggcacaaaca ttcattttat catttgttgg tattcttctt ggtctattag gaacagtggg
    36601 aacatccctt gttctacctg atgcggttcc attccaaagc aattggctgt tttttggcgt
    36661 cattagcttg ttgatgctgg tagtagctgt gttaggcgca ctattttctg ttcggacaat
    36721 cgtaaaaatc gatccgttga aggcaattgg ataagaagga ggaagagcat gaaagcaata
    36781 gaatttaaag aagtagaaaa aagtttccaa gatggcgacc aagtagttaa agcattgaaa
    36841 gaaacaacca tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc ttctggttca
    36901 ggtaaaagta cttttttgac tttggcaggc ggattgcaga cgcctagcag tggctctgta
    36961 tttatcaatg atcaagaatt cagtaatcaa aaagaaagtg aacgcgttaa actacgtttt
    37021 aaagaaattg gctttatttt gcaagcttcc aatctggttc cttttctgac agtaaaaaag
    37081 cagttattat tagtagataa gatcaaacat gaacatcgtc aaaaagaagc aaaagaatta
    37141 tttgagcagc ttggcgtgga taagttgatc aacaagtatc cagaagaact atctggtgga
    37201 gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat tattctagca
    37261 gatgaaccaa ctgctagttt ggattcagaa aaggcacgag aagtagtcga tatcttggca
    37321 aaagaatcaa aagacaaaaa taaagcaacg atcatggtta ctcacgatac acggctgatt
    37381 gacaaatgcg atagaatatt tatgatggaa gatggtgtat tgaaagaaaa agaataacca
    37441 aaaagcaggt ggaaaaatgg tccatcgata caacaaagat acacagcaaa aaattttgaa
    37501 agaagcgact gacttgttta tgtccaaggg atatctcggc acttcaacaa gagagatcgc
    37561 acaaaaagtt gggatcaccc aacccaatct ctatcattat ttcggtgata aagaaaaact
    37621 atatacggca gttttagaaa accacttaaa agatgttgga aaagctttga gagaaatcgt
    37681 tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt ttttgattga
    37741 gacacatctg gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc tttcaaagga
    37801 aacaagagat catcttttct ttctatggaa gaaaaattat cgtgaacctt ttgaagaaat
    37861 cttttcagca aaccaatctg tgctaagaaa tgggatttca aaagaaatcg cagcaaggca
    37921 ttttttctta gtgttagcac cttacatcac tcggtcgaaa agacctgtgg aagaaccgct
    37981 aaatgtggcg caattgatcg atttatattt gaatggtgta ttaggagaag cgtaaagctg
    38041 ttatataggg agaaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct
    38101 tgtttttttt atataggtag tttttcataa atacaaattt gttattctct catgattatc
    38161 agggtatatt tttatttatt atgtcggtgt tccaggaagt gttttaattt aataacgaag
    38221 aaattaattg tttgtagaat gaaggaaact atttattggg tagatgaatc accacgaata
    38281 gtcaaaagga gatttcagga tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta
    38341 ttttctacgg ttatatcttt tacagcccct tttttgcgta cttaagctga ttttaagttt
    38401 tgacccttag tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt
    38461 tgtatttgca atcattatca ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa
    38521 gatagttgga caaacggtca gcagtacgaa cagtacggtt acaaagacaa aggaatcatt
    38581 tgatgataca gaacaagaga cattaatcaa aatcggaaaa aatgaccgta cactatatac
    38641 agatgactcg ctaactaccc cacttgcaac tataaacggc ggagagctgg cggagtttct
    38701 ttctgaaaca aaaaatgctt atcagatcaa gacaaatgat ggctatactg gatatttagc
    38761 cctcgaagat ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag agctatctga
    38821 tgcagtgatt gtattagatc ctgggcatgg cggaaatgat actggggcat taagcaatga
    38881 tgaacaaaca gaagaaaaag atataacatt aagtacagcc ataaaagtaa aaaaggcgct
    38941 agaggatgct ggagcaacgg tttatttgac ccatactaca gatgaattag tacagcttgg
    39001 agatatatgc gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga
    39061 ctctacggaa tatgcgaatg aagcaactgg aatcacgaca tattattact atggacaaga
    39121 ggaaacatta gcacagacaa ttgctgactc cttcactgat ctgcctctgg attcaagagg
    39181 aatctccact gggaactatc aagttcttcg agaaaatctt caaccttcta ttttaatcga
    39241 gatgggttat atgaataatg atagtgattt aaaggaatta gtaacagaca gttatcagca
    39301 gcagattgct gcaagtttga cacaagcatt gaccacttat tttcagtaat aaataagccc
    39361 agccaaacaa aaatagctcc tcatattttt cgtttggccg ggcttattat cgcagatgca
    39421 agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc cgcgtcacaa
    39481 ggagcaattt caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga
    39541 gttggcatga gaacaccgtt tattcggaac aagggactgt gacatgactt ttgtcacagt
    39601 tccattattt ttttagcagc ttcaaagctt tttctgtatt ggcaaaatga agtttggcat
    39661 atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact tggtcagatt
    39721 tcgttccagc tcctgatgga agggtgattc cagcttcagc tgatgctttg tgtaactctt
    39781 ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga tattgttctc
    39841 ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc gcatattttt
    39901 taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt tctggctcga
    39961 ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc cgaacggcat
    40021 tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt ttatagggga
    40081 tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac tcttttgaat
    40141 ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa gcagcacata
    40201 ctgttagcgg accaaaatag ctgccatttc cgacctcatc actacctaaa acggataatt
    40261 gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt ttatcctgtg
    40321 cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc acttttccgg
    40381 atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta taaggaatct
    40441 gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg tcgtttgttg
    40501 ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa ctataccgca
    40561 actcacgact tttcgttttc taagagattg cgagacatag cattttttta aagtttatca
    40621 tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc aacttcatgg
    40681 tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc acgaaaaaac
    40741 acgctacaaa gcagtgattg ccaaccaaac atatactatt atcggacggg aaaccaagca
    40801 tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga aacaattatc
    40861 accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc tttcggatca
    40921 attgaagaaa caagaaagaa tcttggagct ggaagaagaa acagcagaat taaagaaaaa
    40981 aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa tcgagaacga
    41041 agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa atcatgtgga
    41101 agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat cttctaatta
    41161 agatcctttt atcaaaataa gaaaaattta aaaaagttag aagagaaagg aacgtagcat
    41221 gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag cacgtcgcgg
    41281 gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg ctgcccagca
    41341 tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag ctgtcacccc
    41401 aacatcaaag ttagcatttt ttgatcaggt atttgctttt cacctagatg aagcttttta
    41461 tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc gatttgtggg
    41521 ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact ggattgcagg
    41581 aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc agttacttgc
    41641 ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg ctcgttttat
    41701 cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttggtaac gaatattttg
    41761 aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtagt ttatttaaga
    41821 aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa
    41881 atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa
    41941 tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa
    42001 caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga
    42061 ggcgggatcc ctatacctaa acttgaaaat atccgtcccc atatgaaacg gatcgagatc
    42121 ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc
    42181 gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat
    42241 atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc
    42301 gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac
    42361 attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat
    42421 gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc
    42481 aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag
    42541 actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa
    42601 atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc
    42661 catcgtcaag aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca
    42721 aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat
    42781 gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac
    42841 atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa
    42901 acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt
    42961 cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac
    43021 gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat
    43081 attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca
    43141 gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct
    43201 gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata caatcgggca
    43261 aatacgatta atgccagcat ggagttcgat gtggatacac taagtccaac gtatcgtttg
    43321 ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat
    43381 acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa
    43441 atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt
    43501 cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc
    43561 tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca
    43621 gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt
    43681 gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta
    43741 catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg
    43801 ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt
    43861 aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac
    43921 gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga
    43981 tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa
    44041 gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg
    44101 actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa
    44161 aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg
    44221 acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta
    44281 tagaaatgct atactaatca tagattttaa aaatggaggt ctttgatatg tcacaagtaa
    44341 ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg
    44401 caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat
    44461 atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc
    44521 aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag
    44581 aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat
    44641 aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt
    44701 tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg
    44761 ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac
    44821 cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac
    44881 ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat
    44941 cgtgtccgct catattttac tggcagccat aatacgaaaa cggaacggct tgttagtgaa
    45001 atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc
    45061 aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat
    45121 cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaagtacta
    45181 aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag
    45241 aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct
    45301 tgtctttact atcatttggg acaatgtctg tgtccttatt atttcgacgt agatccgaaa
    45361 gtctatacga aaatcgttga tgaagtgaaa aagtttttaa acggaggaca cgaaaaaatc
    45421 gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct
    45481 gcagaatacc gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca
    45541 aatactgatt tactggatcg agatgtattt ggttatgcga ttgacaaagg atggatgtgt
    45601 gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct
    45661 ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat
    45721 gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa
    45781 gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa
    45841 ttggcgaata agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa
    45901 caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca
    45961 atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg
    46021 gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca
    46081 gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcacga
    46141 gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa
    46201 gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc
    46261 atggcaaaaa acgataaaca taaaacaaat gagttgctat tcggtataga tttggctgta
    46321 gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc
    46381 catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga
    46441 ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa
    46501 tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa
    46561 aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa
    46621 agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt
    46681 aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt
    46741 gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat
    46801 catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct
    46861 gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg
    46921 ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag
    46981 gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt
    47041 agagggaaat caagaaaaga gattgaacca aaaatcgatt tttggatgga aaaatttcag
    47101 gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc
    47161 cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc
    47221 gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat
    47281 ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat
    47341 ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa
    47401 tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa
    47461 atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa
    47521 gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg
    47581 tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat
    47641 gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt
    47701 cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt
    47761 tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag
    47821 tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga
    47881 aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga
    47941 gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact
    48001 actgatacaa caacaactga ctggcttgca atcgatgctt cgagcaagtc aattaggcat
    48061 ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa
    48121 ttttgattca aaaggaaaaa tgacagttgg agaagataac tcgacagtcc aatatgtcgt
    48181 aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc
    48241 acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac
    48301 tgcacaaaag catttttacg gaaaactaac aggtgtttta cttgttgcat taacgcaaat
    48361 ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt
    48421 caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc
    48481 tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc
    48541 tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct
    48601 aggcggatat atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg
    48661 gatcacttcg tatattccat tcttatcttc atatattatg ccggttcgct tagcaaatga
    48721 gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt
    48781 attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtgtata gcgaaggcgg
    48841 attatggagt tcattgaaac aatcgatttc aattatgaga aatgaacgaa aaaaagcata
    48901 aaatatagaa aaatatagaa ggaagctgga catttattgt tcccagcttc ttttttgtat
    48961 agacctattt ctattttttt attatattgg atatatatat tcattgaaaa tagagcataa
    49021 caactttata atgttgagta taccaatttt tggaggcgtt ttaatggacc gctatttaga
    49081 tttactttca gagaagttcc ctacaacgga agatgtcatc acggaaatca tcaatctcga
    49141 agcaattttg cagcttccaa aaggaacaga gttatttttg agcgatatac atggcgaatt
    49201 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    49261 ccttttctct gaacaaatga cggaaacgga gatcagccaa ttcactattt ttacggctta
    49321 tccagaatat gtgttgacga ctgattggta caaaaaacaa gataaatccc agctgatcca
    49381 tcgtttgatc gatcttttac ggtttacgac agtcaaatac actcgatcaa aagttcgaaa
    49441 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgatcgtat
    49501 caacgggaaa aaagactatg taaaaaaaat cattgaacaa cttgtcttga tgaaggaaga
    49561 agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa tctgtcatcg atcatttgca
    49621 tatcgtgggt gatatttttg atcgaggaac tcaagcagcc aaagtaatgg atcgaatcat
    49681 ggatttttcc tctgtagata tccagtgggg gaatcatgat attttgtgga ttggtgcgta
    49741 ttgcggctct gaggcatgtt tattgacgct tttacggatt gccgcacgct acaactattt
    49801 atttgaatta gagaaagaat atggattaaa tttacgtcca ttattttctt ttgcccatca
    49861 aacctatcag aagaatccag tatttacccc taaaaacagg aaagatctat ctgaaagaga
    49921 gcaagaagaa ttagaaaaga tccatcaagc actttctatc ctccaattca aatcagaaca
    49981 tcagttgttg aatcgacgac ctgaatttaa aatggacgat cgtaaattgt tggaaaaaat
    50041 caactatgag gaatcaacga tcgttttaga aggtcagctc tatgagttga aagggacttg
    50101 ttttcaaacg atccagccag atgatcctaa aaaattcttg gaagaggaac aaaaagtgat
    50161 cgatagtttg atgtattcat tccagcactc gttaagaatg caaaaacaca tgacgttttt
    50221 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtactgttcc atggctgtat
    50281 acctgtagat gaaaaggggc agctcttagc atttgaatta gaccaatctt atcgcggaaa
    50341 agaactgctg ggattttttg aaaaacagat aactgaaagc gcaaaagatt tgactgcaaa
    50401 tgaagatttg gcgaccgatt tgatttggta tgcgtggagc gggccatttt cgcctttatt
    50461 cggcaagagt aaaatggcaa cttttgagcg ctattttctc acagagcctc atacgcacgt
    50521 cgaaaaaagc aatccctatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    50581 ggcagaattc gatgcaaaag aagaaggttc agcaatcatc aatggacaca cgccagtaaa
    50641 agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa gcatttgtta ttgacggagg
    50701 tctatccaaa gcttatcaaa aaacaaccgg gattgctggc tactcattac tatgcaattc
    50761 ttacggtttc caaatcgtga cacattatcc gttcttgcct attgaacaac aatttgcaaa
    50821 aaaatctgat caaacgaatg taaaaaaagt gatcgagcag cagctcccac gcaaacttaa
    50881 tcggaatacg acaaaaggaa tggaattaca gcaacagatt actcaactcc aaaggctgtt
    50941 ggattatcga aaggacgatt gagaaaaatg ctagccaaca ttgctcaata gactaaaaag
    51001 cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt agctcaaaaa acggtaaaat
    51061 attagaagct atttttctgt ttatttctct aatactcagg tcagggcgct gatttcacga
    51121 cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaaca
    51181 aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttgaac ggcaggctca
    51241 cgacctaatc agaaaaacga ttaaaattca ttcacaaaga aatcgaataa acgctgttca
    51301 gatgaatcta cacgatgaga aagttccgga tgccattgga caccaaggat tttgtgcata
    51361 ttatctttac tttggactgc ttcaatgagt ccatcgttgg aaaaggcgat ggcttttaac
    51421 aaaggagaaa gatctttgac tgcctgatga tgataggaat tcacaaagta tgaatcaggg
    51481 agaaggtcac ttaataaact gtccgaaacg attttgactt cgtgagttgc aaactgcggg
    51541 gcagtgggct gttgttcgtg ttttacggac cattttggat ataatgaaag atcttgataa
    51601 agtgttccct ccaacacgac attcaatagt tgaagtcctc ggcagactgc aaaaatcggt
    51661 tttctttgtt tcaatgcttc caagatcaat gcctgttcga attggtctcg ttgaatattt
    51721 gtttccgtca gttttggatg agggtcttcc atgtaaagtt gcggactaac atcctgaccg
    51781 cctgcaagaa tgagtttgtc gattttctca attagttgag gcgctgaatc tggagacatg
    51841 actggcagaa taagaggtaa tccttgagca tctaaaactg catccacaaa tccctgcggt
    51901 gtatagctta cttgatttcc ttggaaagta tctgtcgctc gaatcagttg attcccggca
    51961 atgccgatga ttggtttcat ttttattccc cctaaagtag actaattgta acaaggattt
    52021 aaaaaaattt aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta
    52081 tctgtaaaga gaattttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg
    52141 ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct
    52201 tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag
    52261 gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga
    52321 agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gagaagcgtg
    52381 ctcgaaccct acggcagcag ttgaagacct atgcagaaga aaaagagcaa gaattggaga
    52441 taatccaaac gaacagtcgg caacagctga aacagttggt agaagaaaaa gaacaattcg
    52501 aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag
    52561 caactttgaa agaccaatat caaaagcatc agagtcaagc aatcggtgca atcattgaaa
    52621 gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa
    52681 agaaatcagg agaggatttt acaagcagta caaggaatcc aaaatgttga aatccgggat
    52741 ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat attttccaga taccacgatt
    52801 attgataaag aagcaaaatt aagcgcttta ggcaatcggc tgaaagacat ccaagaagct
    52861 atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa
    52921 gaactttcct tacaagactt ggaagcaaat tattccgaag aaaatttcat gaataaatta
    52981 gaagaagtcc ttgatttaaa agaaaagtgg gaacagctaa ctgaaaaacg tgatcgttta
    53041 gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat
    53101 gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc
    53161 aaggaagaac taagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa
    53221 caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgctataga agaaattgct
    53281 gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaattg
    53341 caagaaacca aacagcaatt atccattgtt cacgagcagc aaaaaaagat aacgacagcg
    53401 ttaggtcagt gcagcggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc
    53461 gttgcagaac gggaaaaagt aaaagaacgg tttatccatg ccacttatct gatcgttttg
    53521 caaggttggg tagatgcaga agaaaaacag aatttgatgc atgtgttaaa cgaaactgta
    53581 tcagaagaag atatatacgt tagtttcgaa acaccaaact cagaagagat tcagcaggaa
    53641 gtccctacta aattgaaaaa ccatccgctt atcgaacctt ttgaattgct gactgaaatg
    53701 tacagtctgc caaaatatga agaagtagat ccaacgcctt ggatgacacc attttatctt
    53761 gtcttcttcg ggatgatggt cgcagatgtt ggttacggat tgctgatgct cattgggaca
    53821 atactagctc aaaaactatt tgttttaccc cgcgggatga aacgtttcgt aaaattcttt
    53881 gagattctct cggtaccatc gatcatctgg ggattgatct acagttcatt tttcggacag
    53941 gcattaccaa aagaactatt tggtatccgc ttgcctttcc caatcctatc tacgacggat
    54001 gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat cttggttgga
    54061 ttattcgtgg cagcaaagga aaatatcagg aggaaagatt acttgagtgc tgtcagtgat
    54121 ggttttgcct ggcaagggat cctgattggg atcgttattg cattgattgg tgtcatgttg
    54181 ataaaaaata cagcatttgt ttatcttggc gcggggctat cgatcattgc tgctttatgt
    54241 atcttaatcg tgccgattat ccaatcgccg tctaaagtca agggtgctgc caaaggagcg
    54301 tataatcttt acggattgac tggttatatc ggtgatttag tcagttatac acgtttgatg
    54361 gcactaggta tttcaggcgg aagtattggc gcggcattca atatgctagt tgctttcatg
    54421 ccgccagccg ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat
    54481 atgtttttga cactgttaag tgcctatgtc catggtgcac gattgcagta tgtcgaattt
    54541 tttggaaaat tttatacagg aggcggacgt gcctttgacc cgctgaaaac ggcggaaaaa
    54601 tacgtcaaca tcaatcataa gaaaaaaaac aataaataat tggaggattt ttatacaatg
    54661 atggattact taattagtca aaatggtgga atggtatttg cagtattagc gatggcaaca
    54721 gcgacgattt tttcagggat tggttcagct aaaggtgttg gaatgacagg tgaggcagca
    54781 gcagcgttaa caacaagtca acccgaaaaa ttcggacagg ctttgattct gcaattgctt
    54841 ccaggtacgc aagggttgta tggattcgtt atcgcctttt tgatttttat caacttagga
    54901 aatgatatgt cagttgtaca aggattgaac tttctaggcg cttctctgcc gattgctttt
    54961 acaggtttgt tttcaggtat cgcccaaggg aaagttgcag cagcaggtat ccaaatttta
    55021 gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatat
    55081 gcgattttag gtttcgttat ttccttccta ttagtattga atgcctaagc ataaaggagg
    55141 aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaatgaagca gcagagcaag
    55201 aaagagcggc gttagagcaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac
    55261 gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttacttgaaa
    55321 aaaaataccg tcagcttagg aatcgccaac aagtagaagt acgacaagaa aatttgaatg
    55381 cgaagcagga atttttacga cgattgtttg cagatgcaat agctgaaatg gagaattggg
    55441 atgaatctga acaaatccag tttatcaaaa attcattgta ttcattacct ttgacaggca
    55501 aagtaacatt cattgctgga gaaaaatcag cagcttatct ttctcagaca ttgctggatg
    55561 aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag
    55621 cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac
    55681 aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga
    55741 tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt
    55801 gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat
    55861 attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca
    55921 gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc
    55981 tgaaatcgtt tggatctata cgatgcgcta tactttccat aatctgaagg ttttgacaaa
    56041 agccgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt
    56101 agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaactccctg aaagcattat
    56161 ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga
    56221 cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata
    56281 tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc
    56341 tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg
    56401 aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa
    56461 ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatct
    56521 tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc
    56581 acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga
    56641 aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaac cagaaacaat
    56701 cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt
    56761 ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg
    56821 ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc
    56881 aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta
    56941 tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa
    57001 acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc
    57061 aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg
    57121 ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga
    57181 tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg
    57241 aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac
    57301 aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt
    57361 tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac
    57421 caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa
    57481 caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa
    57541 tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg
    57601 acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa
    57661 aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag
    57721 tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc
    57781 agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc
    57841 gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac cctaatacag
    57901 gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag
    57961 ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt
    58021 atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga
    58081 gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc
    58141 ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag
    58201 gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca
    58261 cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa
    58321 aaagacattt tccttcgatc gattggatac aaagctattc tctttattca acagaagtag
    58381 ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc
    58441 gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc
    58501 tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc
    58561 agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt
    58621 tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt
    58681 taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata
    58741 tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc
    58801 aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa
    58861 gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc
    58921 gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat
    58981 aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta
    59041 cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc
    59101 gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat
    59161 attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca
    59221 ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt
    59281 tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta
    59341 ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa
    59401 gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt
    59461 atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact
    59521 gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg
    59581 accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga
    59641 agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga
    59701 atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat
    59761 aaaactcatc caatccctga cttgactggt tatattacag aaggacagat catcttgact
    59821 agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg
    59881 ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat
    59941 cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt
    60001 gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa
    60061 gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg
    60121 tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg
    60181 gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc
    60241 cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta
    60301 ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac
    60361 cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca
    60421 aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt
    60481 gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa
    60541 tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa
    60601 ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa
    60661 atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat
    60721 gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag
    60781 ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc
    60841 actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa
    60901 taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag
    60961 ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta
    61021 tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata
    61081 cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg
    61141 gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc
    61201 tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga
    61261 agctcatgct ttacatccta aaattggcct ttgttatcca attgattggt acggttgctt
    61321 tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc
    61381 atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc
    61441 accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag
    61501 gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt
    61561 caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt
    61621 tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca
    61681 ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata
    61741 tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt
    61801 cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga
    61861 tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt
    61921 tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt
    61981 tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct
    62041 tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag
    62101 ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct
    62161 tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg
    62221 tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat
    62281 aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag
    62341 gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga
    62401 tttgattcct ttatttatca ataggttata attcatattt ttttattttt tcttttacga
    62461 tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg
    62521 catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa
    62581 gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt
    62641 aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc
    62701 tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt
    62761 gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt
    62821 ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa
    62881 taaattttgt cattccagct aagtagattg tttggttagg taataaattt tggaaaggag
    62941 gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc
    63001 atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa
    63061 accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac
    63121 attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct
    63181 ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta
    63241 ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg
    63301 aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg
    63361 cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat
    63421 ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag
    63481 aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg
    63541 actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt
    63601 tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag
    63661 ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt
    63721 gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc
    63781 gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt
    63841 gtataccata caattttacc attgattgta ttgattttag aagttaatct tttgtgagga
    63901 gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag
    63961 aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa
    64021 atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc
    64081 ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata
    64141 atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg
    64201 gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat
    64261 ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag
    64321 ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag
    64381 tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac
    64441 agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg
    64501 aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg
    64561 aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat
    64621 cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg
    64681 gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat
    64741 taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt
    64801 tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat
    64861 tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga
    64921 ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac
    64981 gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa
    65041 ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc
    65101 gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa
    65161 attttctact caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga
    65221 ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg
    65281 ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa
    65341 aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg
    65401 agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa
    65461 attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg
    65521 ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga
    65581 tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc
    65641 taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt
    65701 ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa
    65761 aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc
    65821 cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt
    65881 agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccggcagctt
    65941 tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt
    66001 ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc
    66061 caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt
    66121 cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa
    66181 cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc aatcataaca
    66241 aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg
    66301 gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa
    66361 aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc
    66421 agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg
    66481 aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg
    66541 tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg
    66601 caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag
    66661 aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat
    66721 tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa
    66781 agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac atattttttg
    66841 attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg
    66901 acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat catggataag
    66961 tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg
    67021 agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc
    67081 gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca
    67141 tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt
    67201 tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa
    67261 gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg
    67321 ccgacacggc gtgccatcac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg
    67381 gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa
    67441 tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg
    67501 ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact
    67561 tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt
    67621 ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc gccattacgg
    67681 atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac
    67741 ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat
    67801 gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc
    67861 tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag
    67921 ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg
    67981 agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta
    68041 cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga
    68101 gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt
    68161 ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga
    68221 tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca
    68281 ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca
    68341 tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt
    68401 cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag
    68461 tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa
    68521 gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc
    68581 tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta
    68641 tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc
    68701 gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt
    68761 gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa
    68821 tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta
    68881 atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg
    68941 acatggatat agttatggac tggatcacga aaaacttttt ccataggcag catttgatat
    69001 ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat
    69061 ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa
    69121 atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac
    69181 aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg
    69241 atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg
    69301 cccgcgtttt tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt
    69361 gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc
    69421 aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt
    69481 agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg
    69541 ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga
    69601 atttctgatc actggagcgt aacctgcatc tttcagtgtt tggatcccgt cacttagaaa
    69661 aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa
    69721 aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga
    69781 aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc
    69841 tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg
    69901 gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac
    69961 aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt
    70021 tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat
    70081 ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag
    70141 gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag
    70201 ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa
    70261 acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat
    70321 gttagtttga aagaccgtcc cacaggaggg aaggttcgaa tcgattatga tctgttcatg
    70381 ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag
    70441 aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg
    70501 tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa
    70561 gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac
    70621 cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt
    70681 tatactgact tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg
    70741 cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag
    70801 gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat
    70861 gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat
    70921 gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct
    70981 gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat
    71041 atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa
    71101 gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga
    71161 cgatcagcct atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta
    71221 tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg
    71281 tacgtcatat taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa
    71341 aacggcagat cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa
    71401 aaatgttccg tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc
    71461 aagatagttt tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta
    71521 gtgcatggtt ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa
    71581 ggacgcctgt ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt
    71641 ccgttgcaga gctccatatg attttagata aaatagacag tccggaaaaa gagattctga
    71701 tcctctactt ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg
    71761 aaaacacagt taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat
    71821 gaaggagaga agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact
    71881 tataaagaac aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc
    71941 gaagagcaat catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta
    72001 tctttggcta tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc
    72061 tttgcagaag atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt
    72121 actgatcgat ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa
    72181 gaaaataaaa caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt
    72241 gaaaaagctg aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat
    72301 tatcagaaaa tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca
    72361 gcagcagata gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag
    72421 ctctcacttc cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc
    72481 aaacggcaga tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa
    72541 gatattcaag aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat
    72601 ttgaacaaag agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat
    72661 atgggtacac cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa
    72721 gattatttgg atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag
    72781 tacaagtaaa taataagaag agaaaaagag aaagaagaga aaaagagaaa gaagagatat
    72841 agtgaaaaga aattatttag ttatcagtat tattgcttta tccgtgcttt tgtttttcga
    72901 aattacttcg gtggcctcct tgatcaccga agtaaaagga caaacacact cttcggagca
    72961 ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt aaaagccaat caactgaaac
    73021 agctaacgtc aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca
    73081 atcaacacaa gacagtacaa aaacgagccg agatccggct gtcaaaagcg tcgcaatcag
    73141 ctttgatgat ggaccaggag cgaccacaac accgcagctt ctgcgaatct taaaagaaaa
    73201 aaacgtgcat gccacttttt tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt
    73261 gaagcagaca gctgaagcag ggcacgagat tggcaattac acatacgatc atcagaatct
    73321 ggccactctt tctgcccagt cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa
    73381 aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg tcacgaatgt
    73441 aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag aagattggaa
    73501 gacaagaaat cctgatttga ttttgcagaa gatccaagca acagtatacg atggggcaat
    73561 tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag tgatcgatta
    73621 tctgaaagag caagggtatc gcatcacgac agttggggat ttattagggc atccaacggc
    73681 tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga gacaagtatc
    73741 tttaataaat aacacatttt aataagtagt tgaacacata actaccttac ttgcaaaggc
    73801 ttaacaatat ctatacaggt acaacaaaaa actgtccacc aattttcttt ggcggacagt
    73861 tttcacttaa aaataaagaa gcaactattc gtaaattatg ctaaaatagc agtagaacgc
    73921 gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata tccactacac
    73981 taaggacgca agcactggga taattgtaac taaacagata aaaaattaca aggggattgg
    74041 acaaaaaaat gaaatttgag caaaattttt cacaaaaaca gcagcagaca cagaaactag
    74101 ccatgacgca acagttgcaa cagtccatcc aagttttaca atataacagt gaggagctgt
    74161 tggcattcgt tgagaatcaa gcaatggaaa atccattggt ggaagttgtt gagccggaat
    74221 ggcagccaga ttacataaaa gcttcttcat cttcttatga aggagaagag acaaattacc
    74281 taaatcagat tcctgataca aaagggtctt tatttgattc attgatcgaa caagtccatt
    74341 tgaattaccg agatactttt ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt
    74401 tgaacggatt tttgacgatt agtttagaag aagccggaaa agagattgga gctacgccta
    74461 tccaaatgct cgatgcgttg actctgattc agcaattaga accagctgga gtaggtgcac
    74521 gtagtttgca agaatgtctc atgcttcaaa cagaacgaga tgattatgcg ccggaattag
    74581 cttatatcgt gttggaagaa tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg
    74641 cacaacgttt ttctgttgat ctacatgctg ttcagcagat attcgactat ttacaaacgt
    74701 tgagtccaag ccctggccgg attttcgacc gtagcagcga attgtacatt attcctgatg
    74761 tccgggtatt agtggacgac gataaaaatg tacaagttat ctcaaaccga aaaaatcagc
    74821 caaatatacg ctttcaagag agttacttca aacaaatgtc acaacaagca gataaagaaa
    74881 ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa acgatcctac
    74941 aacgtgggga tacaattttg cgggttgctc aagtcattgt ttcccggcaa aaagctttct
    75001 ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca actgagatag
    75061 atgtgcatga atccacggtc agtcgtgcag tcaacgggaa atatttggaa acagatttcg
    75121 gtgtatttga attaaaaaaa tttttcacta cacgaattcc tacgaatagt acagaacaga
    75181 cagaagacct ttcagcagat acagcgaaga aaaagctgca ggaattagtc gatcaagaag
    75241 acaaaaacaa gccgttgtcg gaccagaaat tggttgaatt gctgaaaaaa gatgagatag
    75301 cgatctcaag acgaacagta gcaaaatatc gtgatttatt aggaatcccg agttcatcaa
    75361 aaagaaaacg ttatgataac taatcagtca aaagactaga aaaagtattt cactttttct
    75421 agtcttttta gttgaaaaac gagagcgaaa ctgttagact tacagtcgtg aggttgagat
    75481 ttccctttat tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta tatgggtcgt
    75541 tttaagtcta cattggacgt aaagtgtcca actaaggaga tcgctatgct ggatgaaata
    75601 aaaatgattg aatctgttgc accagatatt atcgaagtgt tgcaagaacg ctataaaatt
    75661 ttacgaaata tctattggat gcagccgatt gggcgtcgaa gtttatcgga aagtatgggg
    75721 atcacagaac gagtcttacg aacagaaaca gaccttttga aaaatctgaa tttgatcgac
    75781 acctcaaaaa gcggtatgac gctgacaact aagggcgaag aagtttatca aagccttgaa
    75841 aacttcatgg atcagctttt aggtacgcat caaaccgaac aacagttggc agaatatttt
    75901 ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag agcagaccaa agtggctgat
    75961 gcattcggcg aagcgctgag cgaagcctta gaccgactac ttccagaagg cgagaatatc
    76021 atcgctgtga tgggaggaac tactatggct gtggtagccg aacaattgtc taatctggaa
    76081 aataagaaac ggcataacct gtttgtccct gcaagaggcg ggattggtga agcgataact
    76141 gtacaggcaa attctgtcag tgcaagaatg gctgctaaag caaacggtaa ccaccgcgca
    76201 ctttatgttc cggaacaatt aagtctggca acttacaatt ctttactcaa tgagccatct
    76261 atccaagaag tcttaaactt gattagtgaa gcgaattgtg ttattcatag tattgggcgt
    76321 gcattacaca tggcagcacg tcgtaagatg actgaaaaag aattagtcat gctgaaacaa
    76381 gcaaatgctg ttgcggaatc ttttggctac tttttcaatg aaaaaggtga agtcgtttat
    76441 aaggtcccta gaatcggcat agaattgcaa gatttggaaa aagtcccgat catcatggca
    76501 atcgcgggcg gaaaatcaaa agctaaagca attcgagctt atatgaaaaa tgcaccaaaa
    76561 caaacgtggc tcatcactga tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt
    76621 taaaataaat atttttttga ttttcataag gaggaaatct taaatgacag ttaaagtagg
    76681 tattaatggt tttggacgta ttggacgtct agcattccgt cgtatccaag atgtagatgg
    76741 aatcgaagta gtagcaatca acgacttgac agatgctaaa atgttggcac acttgttaaa
    76801 atacgacaca actcaaggac gtttcaacgg aacagttgaa gttcatgaag gttcattcaa
    76861 cgtaaacgga aaagaagtta aagtattagc taaccgcaac ccagaagaat taccatgggg
    76921 cgaactaggc gtagatatcg ttctagaatg tactggtttc ttcacttcta aatcagcagc
    76981 tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc tctgctcctg gcggaaacga
    77041 tgttccaaca atcgtttata acacaaacca cgaaacatta actggtaaag aaacagttat
    77101 ctcaggtgct tcatgtacta ctaactgttt agctcctatg gctaaaacat tgaacgacaa
    77161 atttggtgtt gttgaaggat taatgacaac tatccacgct tacacaggtg accaaatgac
    77221 tctagacgga cctcatccta aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat
    77281 cgttcctaac tcaacaggtg ctgctaaagc tatcggttta gtaattcccg aattgaacgg
    77341 taaattagac ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa cagaattagt
    77401 aacagttctt gaaaaagaag taactgttga cgaaatcaat gcagcaatga aagaagcttc
    77461 aaacgaatct tacggataca acacagacga aatcgtttct tcagatatcg ttggtatgac
    77521 ttacggttca ttatttgatg ctacacaaac taaagtaatg acagttggcg acaaacaatt
    77581 agttaaaact gttgcttggt acgacaacga aatgtcatac actgcacaat tagtacgtac
    77641 tttagaatac ttcgctaact tataagatag acaaacgtac aagtgtaaat tatagacatg
    77701 tacatttaaa agcggggaag caatcgcgct tcttccgctt tttttgataa taagtttatt
    77761 caggaggaaa taaaatggct aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag
    77821 ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg cgtgatcact gacgacactc
    77881 gtatcaaagc agcattgcca acaatcaact acgttctaga acaaggcgga aaagcaatcc
    77941 ttttctctca ccttggacgt gtaaaaacag aagaagataa agaaggaaaa tctttagctc
    78001 cagttgcaaa acgtctaggc gaattattag gtaaagaagt aacattcgta cctgaaactc
    78061 gcggagaaca attagaagaa gcaatccgca acatgaatga cggcgacgtt gtcgtattcg
    78121 aaaatacacg atttgaagat attgacggta aaaaagaaag caaaaatgat acagaactag
    78181 gtaaatactg ggcttcatta ggcgatgtat ttgttaacga tgctttcggt acagctcacc
    78241 gtgcacatgc ttctaacgta ggtatcgctt caactggtat cccaacagtt gctggattct
    78301 tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga
    78361 ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc
    78421 ctaaagcaga taaaatcttg atcggtggcg gaatgactta cacattctac gaagcaaaag
    78481 gaatcaagat cggtaactct ctagtagaag ctgataaagt ggaactagca aaagaattaa
    78541 tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt
    78601 caaatgatgt tgaaacacaa gtaatcgaag gagacattcc tgatggtttg atggctttag
    78661 acatcggccc agcatcagta aaattatttg ctgatacatt acaaggtgca aaaacagttg
    78721 tttggaacgg acctatgggt gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg
    78781 tttgtgaagc aatcgctaac ttagaaggcg caacaacaat catcggtggc ggagactcag
    78841 ccgcagcagc agaacaatta ggatttgctg acaaattcac acacatctca acaggtggcg
    78901 gcgcaagctt ggaattgctt gaaggtaaag aattaccagg actagcagca atcaacgata
    78961 aataaacgac caagtttgct caattctgac gagtaatcga acaaaaggtg aagggagttt
    79021 tcatgactcc ctagccattt ttcaataaat taaggagtgt gttcctcaat gcgtaaacct
    79081 attatcgcag gtaactggaa aatgaacaaa actgcatcag aagcaaaaga atttgctgaa
    79141 gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg atctcctgca
    79201 ttattcttac aagaattaat gacatcagct gaaggtacag aactaaaaat ctcagcacaa
    79261 aactgctact gggaaaactc aggtgcgttc actggtgaaa cttcaccagc agctcttgct
    79321 gatctaggtg ttcattatgt gatcatcgga cattcagaac gtcgtgaata tttccatgaa
    79381 acagacgaag acatcaataa aaaagcgaaa gctatctttg caaatggtat gacaccaatc
    79441 ctatgctgcg gcgaatcttt agaaacctat gaagcaggca aaactgctga atggatcgaa
    79501 ggacaaatta cagctggttt agctgatctt tcagctgaac aggtgtcaaa catggttatt
    79561 gcttatgaac caatctgggc aatcggtact ggtaaatcag ctgacgcaca aatcgctgac
    79621 gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa
    79681 gcagttcgta tccaatacgg cggttctgta aaaccagaaa atattgctga atacatggca
    79741 aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg
    79801 tcattgttag acgctgtaaa ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg
    79861 cactaatgag caaaattaac taaaatcgta tatgagggaa atcccttagg aaaaaacaaa
    79921 ctcaaaggag agacaaaaca tgtcaattat tactgatgtt tacgctcgcg agatcttaga
    79981 ctcacgtggt aacccaacaa tcgaagtaga agtatacaca gaaagcggag cttttggccg
    80041 cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg
    80101 agacaaagct cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg taaacaacat
    80161 cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa atggctatcg acaaagctat
    80221 gatcgcttta gacggaactc ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt
    80281 ttctatcgct gttgcacgtg cagctgctga ctacctagaa gtacctttat accactacct
    80341 aggcggattc aacacaaaag tattgccaac accaatgatg aacatcatca acggcggatc
    80401 acatgctgat aactcaatcg acttccaaga attcatgatc atgcctgttg gcgctccaac
    80461 attcaaagaa gctctacgta tgggtgctga agtattccac gcattagctt caatcttgaa
    80521 agctcgcgga ttagcaactt ctgttggtga cgaaggtgga tttgctccaa accttggttc
    80581 aaacgaagaa ggttttgaag taatcatcga agcaatcgaa aaagctggct atgtacctgg
    80641 taaagacgtt gttcttgcta tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg
    80701 tgtttacgta ctagctgact caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt
    80761 ctacgaagaa ttagtttcta aatatccaat catctcaatc gaagatggat tagacgaaaa
    80821 cgactgggac ggattcaaga aattaactga cgtattaggc gacaaagttc aattagttgg
    80881 tgacgacttg ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa aaggaattgc
    80941 taactcaatc cttatcaaag tgaaccaaat cggtacatta acagaaactt tcgaagctat
    81001 cgaaatggct aaagaagctg gctacacagc agttgtatct caccgttctg gtgaaacaga
    81061 agattcaaca atctctgata tcgctgttgc aacaaacgct ggccaaatca aaactggttc
    81121 tctatcacgt actgaccgta ttgctaaata caaccaatta ctacgtatcg aagaccaact
    81181 tggtgaagtt gcagaataca aaggtttgaa atctttctac aacttaaaaa acaaataatt
    81241 cttttctaat gaagatacat gatcgactat tgaaagatag tcgagtatga tcacttagtt
    81301 aagtaatcga actctctact tgctgaaata tcagcaggta gagagttttt ttgcaactag
    81361 cttctgtata tgatgtttac aaagaatggt acaagaaaag atcatccttg aaaaaggaaa
    81421 gctaacaata actagcatca tataagagct tttaaggata gaacagtaga ataggaatct
    81481 atgggcaagt cctctcgtat aaataaaata ggagggaagt agcagcatat ttttcataaa
    81541 gtggctctct ttcttcattt ttactttggt tgctcctatt tgaaaattcg agcacactca
    81601 aatacaataa aaagaaatgg caagggagag gattagatga aaaaagaaga aaaaatcgcg
    81661 attttacaag agattatccg aattaaatca gtgaatggaa acgaaggtga agtagcagcc
    81721 tatttgaata agcttttggc aaagcatgac ataacaggag agattgtttc ttacagagat
    81781 ggtagagata atttgattgc tcgttaccaa aagggccaat caggaaaagt gttaggccta
    81841 tctggacata tggatgtcgt ggcggctggt gatgaatcgt cttggaccta tgcacccttt
    81901 gcagcagaaa tccatgggaa ccgtttgtac ggacgtggtg ctacagatat gaagagtggc
    81961 ttggcagcaa tggtgattgc catgatcgaa ctgaaagagt caggaaaacc atttaacgga
    82021 acagtcaaat tattagccac tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc
    82081 acgaaagcag gctatgtaga tgacttagat gccttgatca tcggagagcc aaccaattac
    82141 agtttgatgt atacgcacat gggatcaatc aactatacag tcacctcgca cggaaaagaa
    82201 gcgcatagct ctatgccaga ccaaggttat aatgcaatca atcacctaaa tgaattcatt
    82261 acgaaagcaa atgctgaaat gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga
    82321 aaaacaatcc ataatgttac attgatcagt ggcgggaatc aagtcaacag catcccaagc
    82381 catgcgcagc tccaagggaa cattcgatcg attccagaat acccaaatga taaaatcatt
    82441 gcattgctgc aatcaatcgt caatgaattg aatcaagaga cagactatca tttggaattg
    82501 atgatcgatt acaacaaaat acctgtcaaa gctgatccag attctccttt gattcattgc
    82561 attcagcaac aattcagcca gccattacct ttagttggag cggcagcaac aacggatgca
    82621 gccgaattta cgaaagctaa ccattccttc gactttgtcg tctttgggcc tggtgtcgtt
    82681 actttgcctc atcaagtaga tgagtatgtt gaaatcgata attatttgga tatgatcgaa
    82741 aaatatcaag gaattatttt atcctactta gcgtaagatt tttttaa
//
LOCUS       DACECB010000004        80225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECB010000004 DACECB010000000
VERSION     DACECB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.004"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            172..444
                     /locus_tag="IWO04_000291"
     CDS             172..444
                     /locus_tag="IWO04_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211930.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            459..1382
                     /locus_tag="IWO04_000292"
     CDS             459..1382
                     /locus_tag="IWO04_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0211931.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1522..2274)
                     /locus_tag="IWO04_000293"
     CDS             complement(1522..2274)
                     /locus_tag="IWO04_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0211932.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2497..2859
                     /locus_tag="IWO04_000294"
     CDS             2497..2859
                     /locus_tag="IWO04_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0211933.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3496..4968
                     /locus_tag="IWO04_000295"
     CDS             3496..4968
                     /locus_tag="IWO04_000295"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0211934.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4980..5804
                     /gene="nadE"
                     /locus_tag="IWO04_000296"
     CDS             4980..5804
                     /gene="nadE"
                     /locus_tag="IWO04_000296"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0211935.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5877..6767)
                     /locus_tag="IWO04_000297"
     CDS             complement(5877..6767)
                     /locus_tag="IWO04_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0211936.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6965..8482
                     /locus_tag="IWO04_000298"
     CDS             6965..8482
                     /locus_tag="IWO04_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0211937.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8472..9743
                     /locus_tag="IWO04_000299"
     CDS             8472..9743
                     /locus_tag="IWO04_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0211938.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9918..10814
                     /locus_tag="IWO04_000300"
     CDS             9918..10814
                     /locus_tag="IWO04_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0211939.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10828..11691
                     /locus_tag="IWO04_000301"
     CDS             10828..11691
                     /locus_tag="IWO04_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0211940.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11697..12260
                     /locus_tag="IWO04_000302"
     CDS             11697..12260
                     /locus_tag="IWO04_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0211941.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12270..13793
                     /locus_tag="IWO04_000303"
     CDS             12270..13793
                     /locus_tag="IWO04_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0211942.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13805..14764
                     /locus_tag="IWO04_000304"
     CDS             13805..14764
                     /locus_tag="IWO04_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0211943.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14850..15929
                     /locus_tag="IWO04_000305"
     CDS             14850..15929
                     /locus_tag="IWO04_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0211944.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16181..17581)
                     /locus_tag="IWO04_000306"
     CDS             complement(16181..17581)
                     /locus_tag="IWO04_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0211945.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17799..19460
                     /locus_tag="IWO04_000307"
     CDS             17799..19460
                     /locus_tag="IWO04_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0211946.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19417..20115
                     /locus_tag="IWO04_000308"
     CDS             19417..20115
                     /locus_tag="IWO04_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0211947.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20131..21555
                     /gene="araA"
                     /locus_tag="IWO04_000309"
     CDS             20131..21555
                     /gene="araA"
                     /locus_tag="IWO04_000309"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0211948.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21862..23021
                     /locus_tag="IWO04_000310"
                     /pseudo
     CDS             21862..23021
                     /locus_tag="IWO04_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23139..24998
                     /locus_tag="IWO04_000311"
     CDS             23139..24998
                     /locus_tag="IWO04_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0211949.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25108..25542
                     /locus_tag="IWO04_000312"
     CDS             25108..25542
                     /locus_tag="IWO04_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211950.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25981..27003)
                     /locus_tag="IWO04_000313"
     CDS             complement(25981..27003)
                     /locus_tag="IWO04_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0211951.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27131..27484
                     /locus_tag="IWO04_000314"
     CDS             27131..27484
                     /locus_tag="IWO04_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0211952.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27496..28662
                     /locus_tag="IWO04_000315"
     CDS             27496..28662
                     /locus_tag="IWO04_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0211953.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28754..29374)
                     /locus_tag="IWO04_000316"
     CDS             complement(28754..29374)
                     /locus_tag="IWO04_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0211954.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29566..29862)
                     /locus_tag="IWO04_000317"
     CDS             complement(29566..29862)
                     /locus_tag="IWO04_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0211955.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30066..30716
                     /locus_tag="IWO04_000318"
     CDS             30066..30716
                     /locus_tag="IWO04_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0211956.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30732..32459
                     /locus_tag="IWO04_000319"
     CDS             30732..32459
                     /locus_tag="IWO04_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211957.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32456..34348
                     /locus_tag="IWO04_000320"
     CDS             32456..34348
                     /locus_tag="IWO04_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211958.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35052..35921
                     /locus_tag="IWO04_000321"
     CDS             35052..35921
                     /locus_tag="IWO04_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0211959.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36130..37155
                     /locus_tag="IWO04_000322"
     CDS             36130..37155
                     /locus_tag="IWO04_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0211960.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37194..38144
                     /gene="manA"
                     /locus_tag="IWO04_000323"
     CDS             37194..38144
                     /gene="manA"
                     /locus_tag="IWO04_000323"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0211961.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38238..39941
                     /locus_tag="IWO04_000324"
     CDS             38238..39941
                     /locus_tag="IWO04_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0211962.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39943..41976
                     /locus_tag="IWO04_000325"
     CDS             39943..41976
                     /locus_tag="IWO04_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0211963.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41987..42427
                     /locus_tag="IWO04_000326"
     CDS             41987..42427
                     /locus_tag="IWO04_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0211964.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42627..43754)
                     /locus_tag="IWO04_000327"
     CDS             complement(42627..43754)
                     /locus_tag="IWO04_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0211965.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43757..44434)
                     /locus_tag="IWO04_000328"
     CDS             complement(43757..44434)
                     /locus_tag="IWO04_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211966.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44698..46464
                     /locus_tag="IWO04_000329"
     CDS             44698..46464
                     /locus_tag="IWO04_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0211967.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46479..46916
                     /locus_tag="IWO04_000330"
     CDS             46479..46916
                     /locus_tag="IWO04_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0211968.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46929..48080
                     /locus_tag="IWO04_000331"
     CDS             46929..48080
                     /locus_tag="IWO04_000331"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0211969.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48142..48840)
                     /locus_tag="IWO04_000332"
     CDS             complement(48142..48840)
                     /locus_tag="IWO04_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0211970.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48833..50185)
                     /locus_tag="IWO04_000333"
     CDS             complement(48833..50185)
                     /locus_tag="IWO04_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0211971.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50408..51808)
                     /locus_tag="IWO04_000334"
     CDS             complement(50408..51808)
                     /locus_tag="IWO04_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0211972.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51969..52706
                     /locus_tag="IWO04_000335"
     CDS             51969..52706
                     /locus_tag="IWO04_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0211973.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52831..53310
                     /locus_tag="IWO04_000336"
     CDS             52831..53310
                     /locus_tag="IWO04_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0211974.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53307..54074
                     /locus_tag="IWO04_000337"
     CDS             53307..54074
                     /locus_tag="IWO04_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211975.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            54304..54873
                     /gene="lepB"
                     /locus_tag="IWO04_000338"
     CDS             54304..54873
                     /gene="lepB"
                     /locus_tag="IWO04_000338"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0211976.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54977..55579)
                     /locus_tag="IWO04_000339"
     CDS             complement(54977..55579)
                     /locus_tag="IWO04_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0211977.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55685..56154
                     /locus_tag="IWO04_000340"
                     /pseudo
     CDS             55685..56154
                     /locus_tag="IWO04_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56260..56844
                     /locus_tag="IWO04_000341"
     CDS             56260..56844
                     /locus_tag="IWO04_000341"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0211978.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56847..57920
                     /gene="prfA"
                     /locus_tag="IWO04_000342"
     CDS             56847..57920
                     /gene="prfA"
                     /locus_tag="IWO04_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0211979.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57913..58752
                     /gene="prmC"
                     /locus_tag="IWO04_000343"
     CDS             57913..58752
                     /gene="prmC"
                     /locus_tag="IWO04_000343"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0211980.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58745..58924
                     /locus_tag="IWO04_000344"
     CDS             58745..58924
                     /locus_tag="IWO04_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211981.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58946..59971
                     /locus_tag="IWO04_000345"
     CDS             58946..59971
                     /locus_tag="IWO04_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0211982.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60146..61390
                     /locus_tag="IWO04_000346"
     CDS             60146..61390
                     /locus_tag="IWO04_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0211983.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61543..62490
                     /locus_tag="IWO04_000347"
     CDS             61543..62490
                     /locus_tag="IWO04_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0211984.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62624..63253
                     /gene="upp"
                     /locus_tag="IWO04_000348"
     CDS             62624..63253
                     /gene="upp"
                     /locus_tag="IWO04_000348"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0211985.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63392..63634)
                     /locus_tag="IWO04_000349"
     CDS             complement(63392..63634)
                     /locus_tag="IWO04_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0211986.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63755..63827
                     /locus_tag="IWO04_000350"
     tRNA            63755..63827
                     /locus_tag="IWO04_000350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63788..63790,aa:Thr,seq:cgt)
     gene            complement(63946..64584)
                     /locus_tag="IWO04_000351"
     CDS             complement(63946..64584)
                     /locus_tag="IWO04_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0211987.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64707..65339)
                     /locus_tag="IWO04_000352"
     CDS             complement(64707..65339)
                     /locus_tag="IWO04_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0211988.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65690..65989
                     /locus_tag="IWO04_000353"
     CDS             65690..65989
                     /locus_tag="IWO04_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0211989.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66484..68649
                     /gene="acm"
                     /locus_tag="IWO04_000354"
     CDS             66484..68649
                     /gene="acm"
                     /locus_tag="IWO04_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0211990.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68921..69406)
                     /locus_tag="IWO04_000355"
     CDS             complement(68921..69406)
                     /locus_tag="IWO04_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0211991.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            69886..70527
                     /locus_tag="IWO04_000356"
     CDS             69886..70527
                     /locus_tag="IWO04_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0211992.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70546..71181
                     /locus_tag="IWO04_000357"
     CDS             70546..71181
                     /locus_tag="IWO04_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0211993.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71199..72014
                     /locus_tag="IWO04_000358"
     CDS             71199..72014
                     /locus_tag="IWO04_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0211994.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72333..73769
                     /locus_tag="IWO04_000359"
     CDS             72333..73769
                     /locus_tag="IWO04_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0211995.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73958..76405
                     /locus_tag="IWO04_000360"
     CDS             73958..76405
                     /locus_tag="IWO04_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0211996.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76635..77702
                     /gene="rlmN"
                     /locus_tag="IWO04_000361"
     CDS             76635..77702
                     /gene="rlmN"
                     /locus_tag="IWO04_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0211997.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77895..78998)
                     /locus_tag="IWO04_000362"
     CDS             complement(77895..78998)
                     /locus_tag="IWO04_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0211998.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWO04_000363"
     CDS             79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWO04_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0211999.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        26821 a        13796 c        16507 g        23101 t
ORIGIN      
        1 attcactaat aataaagata tatttacagt atcattataa tgtattcaat ttaaaataag
       61 agactttggt aataatgaaa gaggaataaa acttttcttt ggcaaaacaa actccttgct
      121 atgagacgcg tttcatacac tatactcaaa gtatcaaaag gagggcttgc aatgaatact
      181 tatgtagaga taacagatgg cacaacgaac aattattttt atgcgtttgt cgagttcaag
      241 ggatccgtat taacgttgta cagttttcaa gggttgaata ggaatatagt aaaagaaata
      301 ccaatgaatg aaatcgaaaa tttgacaaaa gatatatatt ggggcgggca gcggatcagt
      361 ttttcgcatg aaggaaaaat gtatcagttt tttgaatgcg gtccagctgt ggtagactat
      421 ttacaagaaa atctttttgt ataggtggcg aagcagcagt ggcaaacatt cgagatatcg
      481 caaagctcac aggttattca gtttctacta tttcacgtgt aatcaatgga catccatatg
      541 tagatgagga aaaaaggaaa gaaatacttg cggtcatgaa agaagtcgat tatatcccta
      601 atgcaaatgc cagacagtta agttatggaa aaacgaaaag cattggagtg attctgcctt
      661 ataccaatca tccgtatttt gatcagttga tcagtgggat cattgaagca gcgtttgatt
      721 atgattacaa agtgacactt ttaccgacgg gctatcataa agaaaaagag cgaaggtatc
      781 ttgaagaatt tgcagctaaa gctttcgatg gattgatcat tacgtctcgt gccaatacac
      841 tagatgattt attggcatat caaaaatatg gacctctagt tttttgtgaa gaaatcaagg
      901 gaaaacaggc aagctgtgtc tttattgatc gagaacaatc aattacagaa ggtcttactt
      961 atttgaagca gcaaggtata aagcaattgg gcatcacgtt ggggcgaagt ggcaggctaa
     1021 gttataactc aaaaatcact ttgcagctat gtcatcaact ttttccatct tttgatgaat
     1081 cacttgtttt ttgggattgt atagatgcag agaaaggaaa acaagcaggc ttctttttta
     1141 aagagcttgg agttgatggt gtctttacaa attcggatat ggttgcagca ggcatcttgc
     1201 aaagctatct gcaagataag acaccagtgg tgattggacg cgataatttg ctgatcagcg
     1261 agcttcttgg tttcccaacc atcgaccacc atctggaagc ttgcggagaa atggcatttc
     1321 aattgtttct tacagaaaaa aaggataaag tgaaaatacc ttatacgttt attcaacgat
     1381 gaatatattt aaagtaggat gaaaaaactt gtgaaagaat ggagcagaca ccaaaagtta
     1441 gcataaaaat ctaacttggg tgtctgctgt tttgttatta ggtcatttta cgtgtacttt
     1501 tttgttggat catgtaaaag attacgcctt tttttggata atttcattta aagcttttgc
     1561 tgcaccatga ccacttcgct cgtcataata tgcagtgaac cgttcatcgg cttcgtacat
     1621 cattcctagt cctttatgcg cttttggctg atattcaggc cagctgtaag agaaccactt
     1681 cttatgagct tggaagatac ggtctgctaa gctttcatct tctggttctt ttaagtaaag
     1741 agtgagatca aataacatct gcttttcaac agtttgcatt tcttcatacg tttctttatc
     1801 catattcatc catttttcat tggatgctgt aacctttttt tttccgtatt tttgtcgaat
     1861 ctcttgtcca taggattctt cattttcttt caacttttgt tgtttaaacc cttcaaattt
     1921 ttctttgtca ctcatttttc ttcctccttc gtacatatca agtgtctgtt ggacagttgt
     1981 caagagggta tcgatttgtg cccgtttttc cagcaacatt tgctgatgct tgattaaagc
     2041 tttctggtgg tcaaaagatg gattgtctaa gacctcttgg atatcatcca acttgaatgc
     2101 cattgttcga taaaataaga tgtgttgcag acgatcgact tcttttttgc cataaatccg
     2161 ataacctgaa gaattgatcc tagctggttt tagcagtcca atctcatcat aaaagcgcaa
     2221 ggttctcgga ctgactcctg atatctctga cattttcttt attgtgtact ccattatttt
     2281 tcctcctgac aaatgtaagc ataatccttt acgtaacgag atggtcaaga aaaaattgta
     2341 tttttttctc acgaaattat gttatactat taagtgataa aaggtaaatc cttttgtctt
     2401 taaaggttca attgtaaccg attccttaat gctaggaaag atggtaacat aacttaagat
     2461 tggatctaaa tcgactatat agaaaagagg aatgagatga agctaatcga aacacctgtg
     2521 aatagtaatt taaatattaa gacattttat cctaaagtag tggaattctt ttttgggaat
     2581 acggctatta attattacaa attattttcg ttagatcgaa cacaactgct attggtagac
     2641 acatatgata aaaagcaagt cgtgatgatc aatacgaaga aaaaaatcac aaggcaagaa
     2701 atcgactatg cgattcatca cgttttaaaa atgacacgag aagatgtcaa agtccatgta
     2761 ggagtgaaac aggagctaga acgtgccgga atacaattca aacgtccaaa taaagatatt
     2821 gtagtgatag aacaaaaaaa tacgaaggat tctatttaat aacgactaat aatcatcttt
     2881 atggtaacct gctccatatc taatgtatac aataaaaaac aaggcgagac tgattgatgt
     2941 ctcgccattt ttgtttccat tctattgaca gctgctttct tactttgtat ctttttaaaa
     3001 acgtgtgata ttttttccgc gagttttttc aacgcaaggt atatcttgtc ataattcgcc
     3061 tttccaaaaa aattcaatac ataataagtt atatccgttt ctttggggat aagaagcaaa
     3121 aaaggaaaga cgtattattg cttcaaaaac ttttgccatt gattatgttg tagagacatt
     3181 tggttatatt tgttcaatca tcggtatctt aacggctacg attgtagtag gaaaatctac
     3241 ttattttatc aagtaagaaa aatagaaaac gctaatttct tcgtgtctcg gatacattga
     3301 ataaaataag tattctttct cgtttaggtt aaataccaat aagaaaggat agttttagca
     3361 gatggagttc atttttagca agaaatatcc gtaaaacatg tgctttttca gaaaaaatgg
     3421 aagatttctt ttcctattaa ttttttttgt tataatgggt aggaaatgaa aacttgccaa
     3481 gaggagtaat acaagatgaa taatacttac ccagacgaca gtttgacgct gcatacggat
     3541 ctatatcaaa tcaatatgat gcagacatac tgggaattag gtcgagctga tttacatgct
     3601 gtttttgaat gttactttcg agaaatgcct tttaaccacg gatacgcggt ttttgcagga
     3661 ttggaacggt tagtcaatta tttagagaat ctgacgttca atgattccga tattgcttac
     3721 ttgcgtggat tagaagtcta tcctgaaggt tttttggagt atttacaaaa ttttgaattt
     3781 aaagcaacag ttcgttctgc tcgtgaagga gaactagttt ttgcaaatga accattaatc
     3841 caagtggaag ggcctttagc gcactgccaa ttagtagaaa cagcactgtt gaatatggtc
     3901 aatttccaaa cgttgatcgc gacaaaagca gctcgtatca aatctgtcat cggagaagat
     3961 ccactattgg aatttggaac tagacgggca caagaactgg atgcagcagt ctggggaact
     4021 agagcggcat atatcggcgg agcagatgca acaagcaatg tacgtgctgg gaagatattt
     4081 gggataccag ctagcgggac ccatgctcat tcgttagtcc aatcttatgg aaatgattat
     4141 gaagcattta tggcctacgc taaaacccat aaagactgcg tgttcttagt cgacacttat
     4201 gatacgttga aatcaggtgt gccaagtgcg attcgcgtgg cgaaagaatt aggtgacaaa
     4261 atcaacttcc aaggcgtacg catcgacagt ggagatatgg cctatatatc taagcgtgtt
     4321 cgggaacaat tagatgcagc cggatttacg gaagcaaaaa tctatgcttc gaatgacttg
     4381 gatgaagcaa cgattttgaa tttaaagatg caaaaagcta aaattgatgt atggggtgtt
     4441 gggacaaaat tgatcacagc ctatgatcag ccagcccttg gtgcagtatt caagctagta
     4501 tcgatcgaag atgatgaagg aaaaatgatg gatacgatca agttatcaag caatgcagaa
     4561 aaagtaacta ctccagggaa aaaacaagtg tggcgcatca ctcgaaattt tgatgggaaa
     4621 tctgaaggag actacgtgac attatgggat gaagaccctc gagaagaaga agctatcttc
     4681 atgttccatc cagtgcatac gtttatcaat aaaactgttc gagactttac agctcgccca
     4741 gttttacaag atatctttat cgaaggaaaa agagtttacg aattacctgc actaaacgaa
     4801 atcaaagaat atacaaaaga aaatctagat tcactatggg aagagtataa acgagacttg
     4861 aatccgcaaa aatacccagt cgatctgtct actgaatgct ggaaccataa aatggctatc
     4921 atggaacgaa tgaaaaaaag cgtagcagaa cttcatacag aagcatagga ggagaataaa
     4981 tgagcctgca gacagaaatt atcaatgaat taggtgtcaa accaacaatt gatccaaaag
     5041 aagaaatacg taaaagtatt gactttatga aagcatattt gaaaaaatat ccgtttctta
     5101 aaacttttgt actaggtatc agcggcggcc aagattctac tttagcaggc cgtttggcgc
     5161 aactaacgat ggaagaaatg cgtgaagaaa caaaagatga aaactatcaa tttatcgctg
     5221 ttcgtcttcc ttatggtgaa caagcagatg aagaagatgc aaaagctgct cttgacttta
     5281 tccagcccga tgttagtctg cgagtaaata tcaaaccagc tgttgatgca caagtacaag
     5341 tattaagcga agcaggtgtg ggaatcagcg attttaacaa aggaaatatc aaagcacgtc
     5401 aacgtatgat tacgcaatat gccgtagctg gagaaagagc aggagccgta ttagggacag
     5461 atcatgctgc agaaaacatt actggattct tcacgaaatt tggtgatggt ggagccgaca
     5521 ttttgccatt attccgtttg gacaaacgac aagggaaaca attgcttcaa gcattgaatg
     5581 caccggaaaa attatacaca aaaattccga ctgcagatct tgaagatggg aaaccaatga
     5641 ttgcagatga agtcgcacta ggtgtcacat ataatgaaat tgatgattat ctagaaggaa
     5701 aagaagttcc cgctcaagca caagaaaaaa tcgaagcatg gtggaacaag acacaacata
     5761 aacgacattt gccaatttcc gttttggatg atttctggaa ataacaataa agcaaaaaca
     5821 gccgtccctt catagggatt ggctgttttt gttttataaa atagaaaaga aaaatatcag
     5881 ctgtagtaaa cttttaattt gatatcctga tcgtaattac cgaatccttt accaaataaa
     5941 gtacagcccc ccacatgttt ggcatcgtct ttcacttcga ttcgcaaatg ccaagtgtct
     6001 tgctctttag ggatgtcgtt gatcgtgatg tttgacaagg gttctccatt catattcgtc
     6061 aagtgtttgg taattcggat cgtcttcagc aagccgtatt gattaagatt atctggatac
     6121 caatcaggcg tatatttacc gcggatatca gcaaaatctc cagggctcgt ccatgttcct
     6181 agctccacac catttaggct gaaagtgata tctgaaggcc aattgtcatt tgaaaaagga
     6241 aattcagaag aaatttccac agaaacttcc aacatctcca atgtatcctc tgtatttaaa
     6301 aaattaggtg cctgatactc gacaaatccg ctggtaaacc agagaatgcg ggcatccatc
     6361 cttcgaggat ccataaaata gcgtggttca tccactttcc cgataaaatc atggatcgta
     6421 gctaaaccgc atgttggttc aattgcataa tttgtatagt gaccaatagg tatcgatgtt
     6481 tctttcgtat caaaagcatt gaatattttt tctggaaaac tgatatcgat tttatcgaca
     6541 cgaaggctcg aaattttttg ttgtcctact ttttctgttt tgatgatgtt tgcttcctct
     6601 agctttttca cgtgcatcgt cgtgatagca ctgctcactc caagttcttt tgccaaatct
     6661 ttgacattca tctttttttt ggaaagtagc tggatgatct tgatccttgt tttactagct
     6721 aatgcttcat ataccggtaa ggattcttcg tttatttgta gttgcatata tatcacctct
     6781 taaaaggttt ttcttatata ttaacatatt tgttaataaa agttatatta aattatttgt
     6841 tttaatcatt attaatattt tggttaatta aaatcgataa aaaataagta agtttaacat
     6901 ttatattatt gacaacgctt tcaacgaggt gtattattca tgtataaatt aaaggaggtc
     6961 agttatggaa gccacaatgt tgattagtag tgtcaagcgt gtaggcaaag ttgatccgcg
     7021 gttgtacagt tcatttattg aacatatggg acgagcagta tatgaaggga tttatcagcc
     7081 agatcatcca tcagctaata aaagtggatt tcgccaagac gtcattcact tggtaaaaga
     7141 attgaacatt cccatgatta gatatcctgg tggaaatttt gtgtcaggat ttcgttggga
     7201 agacagtatt gggcccatag caaatagacc tagaaggttg gatttagctt ggcggacaat
     7261 tgaaacgaat gaagttggga ttcatgaatt ttataactgg tgtcagcatg tgggggccga
     7321 aatcaatatg gctgtcaatc tgggtactcg tggagtagat gcagctcgta atttagtcga
     7381 atattgtaat tttcctaaag gaacttattg gagtgattta aggcgttcga atggtcaaga
     7441 gagccctttt ggaattaaga catggtgttt agggaatgaa atggacggtc cttggcagat
     7501 tggacataaa acagcggaag aatatggacg gttagcagag gaaacggcta aggcgatgaa
     7561 actcgtagat gattcaattg aggtggtttt atgcggaagt tcgaatcggc aaatgcctac
     7621 atttggtgat tgggagttaa cagttcttga tcaagcgtac gatcaaatag attatttgtc
     7681 tctgcatcaa tattatggca accaaaaaaa tgatctttct cattatcttg cacgttcctt
     7741 agacatggat cggttcattt ctgaagtgac agcgatgtgc gatacagtca aggcgaagaa
     7801 acatagtaag aagcagatca acctgtcctt tgatgaatgg aatatttggt atcattcagc
     7861 ggaacaagat gagcaagcag cgccttggca gattgctcct cctttactag aagataacta
     7921 taattttgaa gatgcattac tattaggctg tctattaatc actttattga aacatgcaga
     7981 tagagtaaag attgcttgtc ttgctcaatt ggtaaatgtt attgcaccaa tcaggacaga
     8041 aacaaacgga gaagcttgga gacagacgat ctattatcca tttatgcaag ctgctgtgta
     8101 tggacaagga gaggtgttaa gcccacaaat tcgttcagca aactatgcga cggaagattt
     8161 tgaagaagtg ccgtatttag aaagtatagc cgtaataaat gacaaagaac ttgtcatatt
     8221 tgcagttaac cgtggagaag aagaaatgaa tctaactgtt cacttgtctg aattatcatt
     8281 agagggagtg attgattttt ctgaaatgag tggatttgat acaaaagcaa ctaatcttcc
     8341 taaaagtgaa caagtaaaac caagtcattc aacaaatgta aaagctgaag atgggaaaat
     8401 caattgttta ttgaaaccgc tatcttggaa tgtggtcaga tgtcgtttat cagatgaagg
     8461 aggaacaaaa aatgaaaggt aaaaaatggt taattggctc tttgttggta gctgcaacag
     8521 gtattttagg agcttgcggt ggcggaagtt cagcagaatc tgattctgat aaagaagtga
     8581 ctttgacttt ttggaatggg tttactgcat ctgatggtga aatattgcaa gaaatcgtta
     8641 atgaatttaa taaaacaaat gataaaaaca tcaaaattga aatggatatc atgacttggg
     8701 ccaatttgaa tgaaaaactt cctacagcga tttcttctaa aaaagcacct gactttattg
     8761 cattaaatta tccagatttt gcacagtatg tagaaaacgg agcggtacaa ccattagacg
     8821 atttctggga atatgatgga gtagataaat ctgattttaa agaaacggct attgaattag
     8881 gtgaagtaga aggtaaacag tattttgtcc ccatgcaggt acaaggcatg tatatgtatt
     8941 ggaataatga tttgtttaaa caagctggat tagatactga acaaccacct actacttggg
     9001 aagaattgac agaaatggca ccaaaattaa ctgatagtag taaaaatgta tcaggttttg
     9061 tcttcaataa ggatggaact gcaccgttat ataactggat gttagcaaat ggcggtaaat
     9121 tagtgaacga tgattatact aagtctgaat ttgcgtctcc tgagaacctt aaaacgttaa
     9181 aagcgatcca aaaaatgatc catgtagaca agactggtcc agaatcgatt tctggcgccg
     9241 aaatggacaa tttaatgaat gctggtcaac tagccattga aatcaatggg ccatggttaa
     9301 ataacggatt gaaagcaaac gagattgatt ataatgtgac taccttacca caagggagtg
     9361 attcagacaa aaaaacagct attttagatg gcgtaggata tgccattccg gcaagtacag
     9421 ataataataa aaaagaagct gtctatgagt ttttgaaata ttggaataca actgaaattg
     9481 gtaaaaaatg gagtatcgaa aatggttttc ctgcctactt ggattcagta gcaaaagatg
     9541 aagaagtaaa aaatgatcct attgtatctg aattatcgaa acaaatggac tatgcagagc
     9601 catatttacc aggttttacc caaatctctg cgatcaataa cgatatcatc aatccactga
     9661 ttgaaaaatt attagcagga gaagatcctg agacactcat gaacgatgca gatgatgcca
     9721 tcaatcaatt actgcaaaaa tagtacagaa ctttatacag gatagactgt tggcaagata
     9781 cagatatctg aatgatcgac ctagtatttg cagataggtg ttattgcaaa aatcacagcg
     9841 attgttcgga tatcttattc agattcgttt aaaccaagat tgtatatttt atcaaaatga
     9901 aagatgaggt ggactatatg aacggattca ccaaatattg gaatcgtcct gatcgtgctg
     9961 cgtatttgtt tttaactcca tcttttttag ttttattatt atttactgtc ataccgcttt
    10021 taggaacatt tgctattagt tttacagatt tgaatatttt ctttacatca agaaattttg
    10081 taagttttga aaatttcaca cgtatattta gtgatgaacg agcagttaat tcgttgattc
    10141 attctttata tttcaccgta ctcgaaacgc caacacaaat tatcgttggt ttattagcag
    10201 cagtagcatt atctaaaaat accagattca atcgtttttg tcgttcgaca ttttacattc
    10261 cagttatttg ttcattgacg tcaattgcga ttattttttc tatgttgctt gatccgaatg
    10321 taggggcgat tccttacttg ttttcacaag tcggcttgcc gatccctcaa ttttttcgcg
    10381 atccaaccct tgctatgccg acggtagcca tcatgactgt ttggaagaat tttggtgtta
    10441 ctatgacgat tctcttgact gctattcagg gaatctctcc ctctttgtat gaatctgctc
    10501 aaatggatgg tgcaacaaat cgacagcagt tttttaacat tacgctacct cagatcgttc
    10561 cctccttagg tttttgtatt ttaacgaatc tgattggttc gatgcaagtg tttgaccaag
    10621 tatatgttgc aaccgctggt ggcccacaat ttaagacaga aacagctgtt caatatattt
    10681 acagtcgagg atttacagca ccttatgaat tgggctatgc atcagcactg tcttcggttt
    10741 tgtttatcat tgttgcaact atagctatct ccactaatgt gtacatgtca cggaaagaaa
    10801 aacaaatgaa ataggaggga aacctgtatg gcaccagaaa gctcgattac agttactgtg
    10861 aatagtcgaa aagcagttag tggtaaaaaa atcattcgaa cgattagtat gctagttata
    10921 ttgttggtta ttgcgtttcc ttttttgtgg ctgattattt cttcttttaa acacgaaaaa
    10981 gacattatct catttccgcc tagaatattt gcagatagct ataccctaga taactatatt
    11041 aaggtttgga caacgatccc tttattagat tacatcaaaa atacagtgat ttttgctgga
    11101 gggacagtga tcacttctgt attctttgat tctcttgcgg ggtatgcatt tgctcgtatg
    11161 cgtttcaaag gaaaatcagt tttgttctat ttcgttttgc tgacaatgat gatcccattc
    11221 caagtcttca tgattcctct atttattgaa gtcaatttgc ttggcatgct tgatacttat
    11281 gcaggtttga tcattcctcg tatgacaact gcatttggta tcttcatgat gcgttccttt
    11341 tttattactt taccagattc tttggaagag gcagcacgca ttgatggatt aagtgagttt
    11401 aatatttttt tgaaaatcat gttgccattg agcaaaccta ctcttttatc attagggatt
    11461 ttcacgctga tgaatagttg gaatgacttg ctttatccat tgatattgac aagtagttca
    11521 aaaatgagaa ctttaccagc agggttagct ttatttacag ggcagaacat ttctttttat
    11581 ggacctgtaa tggctggaac ggttatttca atgctgccat tactagtggt ttatattttt
    11641 gcacagaaat attttgttca aggaacagct atgtctggaa tgaaagagtg aggaagatga
    11701 aaatagttga gactaaactt tatggacagt tgattgtggt ttctgattac atcttattag
    11761 gtattctctg ggtaatcgtg tgtttgcctg ttattactat cgtgcctgct acatgtgctg
    11821 ttttttatgt aatggcgcaa tggaaaggtg agggatcagg aaatatcatt tattttttct
    11881 ttcaaggaat aaagcaacat tttggtaaga acttgttcct tagtctgtta acgatcatta
    11941 tttattttct tgttaatcaa ttattagaag aacagacaat cgtactttca attagtggat
    12001 atgtagtttt acttatttat tccatgtttc ttttatcctg gatatatgtg atttctgaaa
    12061 acaaagatat cacattttta caaatttttg aacaaagtgc atggaaagta ttttttgatt
    12121 ttttgagaaa tattctttgt tgcgtattgt tattaggatt tgtactgctc atatttcttt
    12181 tccctccttt tatttttatc tttgctggtg gcgtatggaa acttgtatct attatcttga
    12241 ctataggaaa tgaaaggtag ggaaagctgt tggacgaaga agtgaaagta aaaatcagag
    12301 aggattttac ggtaggagag atcagttctc ggctgtttgg ctcatttgtt gagcatatgg
    12361 gaagtgtcgt ctatacaggg atttatgaac cgggccatga aacagctgat ggtgacgggt
    12421 ttagacaaga tgtcctctct tatgtaaagg aattagaatt aggtgtcatt cgctatcctg
    12481 gaggaaactt tacatctggt tatgattggg tggatactgt aggtaggaaa gaacaacgac
    12541 caaaaaaaat tgatttagca tggcgaggta tagaaccaaa ttcttttgga ctccacgaat
    12601 tttttaaatg gatcagtcat gtacaagcga aaccgatatt cacggtgaat ttaggaacaa
    12661 aaggaattga tgaagcaaaa aatttagtag aatattgtaa ttttccaaaa ggaaccttct
    12721 ggagtgattt aagaataaaa aatgggcaaa aaaagccttt tggcgttaaa ttatggtgtt
    12781 tagggaatga gctagacggt ccatggcaag tgggggctaa gagtgcttat gaatatggta
    12841 ggttagcaaa tgaagcctca aaagtcatga agttagtgga tgattcaatt gaaacaatct
    12901 tagttggaag ctctacgcca cgtttaccat cgtaccctga atgggatcgt atcgcattag
    12961 aagaagctta tgagaatgta gattatttag ctttgcataa ctatattgat aagtatgatg
    13021 aagaagactt gactaaacct cctaaaaatg agcgtgatga tacagcgaca taccttgcaa
    13081 aagcttatcg attcgatcgt caaattgaag aaatcgttgc tacttgtgat tatgtaaaag
    13141 gatatttgag aagtgagaaa acaatgtatc tagcgtttga tgaatggaat gtacatgccc
    13201 aaccggaaaa aagacatagg gattttgaag tcggtagtcc catcgattgg tgttacttta
    13261 ctatggaaga tacattatta tttggttcat tagggctggc tattttacga cacgcagatc
    13321 gcgtaaagat tagttgtcaa tcattgttag taaacacaat acctttaata ctgacagatg
    13381 ctaaaggaca agcatggagg aatccaactt attatgtgat gcaacactta tcgaaatatg
    13441 ctaaaggtca agtattgaaa caacaaatca tttgccctgt ttatgattgt gaaaagataa
    13501 aaaatgttcc ttatgtggac agtgtgatcg tagataacgg caatcagctg actattttct
    13561 tgatcaatcg cacaaatgaa caacaaaaga taacgattga acatgatttc aatttgctag
    13621 atcaaggaga gcactacaca ttaaccagta gcgatctgtt ggacaaaaat acacgtgaag
    13681 aaccagaaaa aataaaacct gaaataaact atttttcaat agaaaaacat gacgaattaa
    13741 atgtagggat agaaaaatac tcttggaatg taattagaat aaagaaaatg tgaggaaatt
    13801 tgttatgatg aagacacttt ataataatcc tatcgttcgg caacgggcag atccttgggt
    13861 atataaacat acagatggtt attattattt tacaggatcg gttccaggct accaagtgat
    13921 tgaattaaga agggcaaagt cactgaatga attagaacat gctgcaagcc tgattgtatg
    13981 gcaggctcat gaagaaggac cgatgagtga attgatctgg gcacctgaag tccattatat
    14041 caatggaaaa tggtatattt actttgctgc aagcaatcat aaaactatac gtgatgaatc
    14101 tcatcatcac cgcatgttcg tacttgaaaa tcagcatata gatccaatga atacagattg
    14161 gatagaaaag ggacaaataa aaacgaaaag ggaaactttc agtttagacg gaacaacttt
    14221 ctcttctcaa ggaaagctat attatgtgtg ggcccaacaa gatcctgaaa ttactgggaa
    14281 ttctaatctt tatctatccg aaatggataa tccttggacg ttaaagggga agcagatcct
    14341 cctttctata cctgagtatg aatgggaaaa acggggtttt tcagtaaatg aaggtccagc
    14401 agtccttact cgcaatggga aaatattcat tacgtattct ggtagtgcaa cagatgaaaa
    14461 ctatgccatg ggcctgctgt gggctgatga gcacaagaat cttttattaa aagaatcatg
    14521 gcataaatta tcggagccag tattcgtttc ttctgaaaaa aataagcaat ttggtcctgg
    14581 tcacaactcg tttacggttt cagaagacgg gtgctttgat actttgatct atcatgctcg
    14641 accagagaag aaccaaaaag gggatccgtt agacaatcct aatcggcacg cgaacgcgca
    14701 atgctttacg tggaatgagc aaggatttcc agaatttggt gaacctgttc cgtatgcctt
    14761 atgagcacaa gaaaccttga tttttgcttg aataaacctt gcagactcgt tatgatatat
    14821 gtacgtacaa ataaaaaggg gcaaggcaaa tggcaaaata tcaagatatt gcagaagaga
    14881 taaaacaaaa gatccttcgt gaggaatacg aagtaaatac aaccattccc tcagaattca
    14941 agctgcaaga aatatatgac gtcagcagac atacgattcg gcaagcaatc gctgtgttgg
    15001 taaatgaggg ctatctgcgt aaagaaaaag ggtcgggcac atatgtagat gacagctata
    15061 agaaagaaaa aacaccattg aacagaacaa aaacgatcgg tgtcatcacc acatatctct
    15121 ctgattatat ttttccgtcg attatccgag gaatcgaaaa tgaattacga gaatctgggt
    15181 attcattgct tttagccagt acgaataacg atcacgaatt ggagaaagag tgtttgaaga
    15241 aaatgattgc ttatggggta gatggtttga tcgttgagcc tacgaaaagt aatcagtata
    15301 acccaaactt agcactttat gttgctttga gagaacaagg cattccgatg gtgatgatca
    15361 atgctgctta tgaagagtta aatgcctctt atatttgttt agatgatgtg aaagtcggtt
    15421 ttatggcagc agaatatttg ataaaaaaag gacatcagcg tctgttgttc atcacaaaaa
    15481 tcgacgatct tcaaggaaaa tacaggatga aaggttttat cagcgcgtgt gaacagtata
    15541 atgtgcgttt tttgacggaa gatatcatta ctttcaccac ggaaacacga gaaacaatcg
    15601 ctgaaaaaac agtacaacgg ttgaaagaag aacaagcttt agatggcatc atttgctata
    15661 acgatcaaat agctagttta atgctagata agttgacaga aaatggctat catattccag
    15721 atgacctatc gatcgtagga acagacgatt cttctttaag ccatttaggg aataaaaaat
    15781 tgacaacatt gacgcatcca aaagaaaagt tgggacaaga tgccgcacgg tggatcgtca
    15841 caacgattga aaaaggacag gcacagccaa atattctcta tgaacctgta ctagttgaac
    15901 gggaaacagt gaaagaaaac gaaaaataag agacgggttt ttaccgcctc ttattgctcc
    15961 tacggttact cgtcgcaatg tttgtgagag cttttgtcat cgctcaaggc tgaacgtttc
    16021 tttcaccacc tctccactaa acggtgttct cctaccaact ggtcacaagt cacaatattc
    16081 atcaaacatg agaagctgtt ttttgaaatt gttccttgtg actggaaact aagcggttcc
    16141 ttcttatttc ttgaagctga ccacttctgt cacaacctct ttaagaatga tggatagaaa
    16201 tacgtttagg ataagttttt ttagatgcac tttgttcgat ttcctctaaa gatttcccac
    16261 gagtttctgg tacgatctct tgaataaata aaacccctaa aaagcagatg atcccaaaaa
    16321 tgccgaaaac gatttcttga gacatagaag cagtcatcac aggaaaaagc aagccaacta
    16381 ggaaagaacc aatccaatta aaagaagagg ctaggccaga tgctcgcccg cggacagcta
    16441 gtgggaatat ttcgcctacg attacccatg tcagtggagc ccatgtgcaa gaataaaatg
    16501 cgacataaat acataaaaac aagagaatca acaacgaatt tgtatgagca tccagtacag
    16561 ttcctaaaac agctggtagg atgaaagaaa gtcccatcac gctaccgcct attttcaata
    16621 aagttcgccg attgaacttg tctgcaataa ccaaaaagag caaagaacca gcaactaaaa
    16681 tgattccttg gatgatcggc cacatcaatg catcgctcgc tgcatgccca gtcgcttttt
    16741 ctacgatcag cggaatatag taaaaaatag cattagctcc ttgaaattgc tggaatactg
    16801 ctacaccaat accggcaatg actaaggaac gatatttttc taaaaacaaa gtatgccaag
    16861 aagttccggt tgtcttttct tgtttgacat tcagctggat ttgttcgaat tcctgttcag
    16921 cttcttttgg tgtccgaata tagttcaata ctctttttgc ctcttccaag cggccagatt
    16981 gcataagaaa acgcggtgac tctggtagag ctaaaacacc gaaaaataaa atcagcgcgg
    17041 ggacagcagc gagccctaac atcacgcgcc aagccatcgt ttcaggcaac ccttttagca
    17101 aataatctgc aacataggaa aggagcatgc cagaagcaat catcacttga ttgatccctg
    17161 acaatcttcc ccttagtcta gcaggagcca tttcagacat ataagcagga actagggcag
    17221 atgcagcgcc aacagctaaa cctagaagaa tccttgctgc aatcagaaaa taagaaccat
    17281 tgtgaggggc agatgcagaa aggacagaac cagccataaa aataagtgca gaaagaagaa
    17341 tcatttttcg tcgtccaagc ttatcagaaa gaagaccaga taaagagccg ccaaggatcg
    17401 cacctaacat gaccgaagac gtgatccatc ctatcaaaga agctttacct gccaaacccc
    17461 aatcatgctg taaaaaaggt aaggcgccgg tcataacacc aatatcatat ccaaatagaa
    17521 tgccgccaaa tgcgccaaaa aaataaataa acgatgagga aacttttttt tcttttttca
    17581 taagtattct ctccttctag aatttcgaga aggcggttga tcagattttg taagcaattt
    17641 cattttataa cttatgtacg tacaaaacaa gtgtttttta ttgtattctt gtaaataact
    17701 gcttgacatg tacgtacaaa agtatacaat aatcattgta agcgctacta aaaagacggt
    17761 ttgatcaggt ttgacgagaa ggagggagtt aaatcattat gaaggaagta aatgcgcaaa
    17821 gtatgttaga tgtcatagat ggtcgtacat ctttagggat tgagtttgga tcaactagga
    17881 tcaaagctgt attgattgat tcatctttta ctcctattgc cgctggtagt tatgagtggg
    17941 aaaacaaact agaaaatggt atctggacct attcattaga agatatttgg aaaggactgc
    18001 gcgtcagtta ccgaaaaatg gcagcagaag tattcgaaat ctatggcgag aatcttacga
    18061 caatcgggtc gattgggttc agtgcaatga tgcacggtta tctagcattt gatagcaagg
    18121 aacagttact agtaccattt cgtacttggc gaaatacaat cactgaagaa gcagaacgaa
    18181 tattaacgga aaaatttcaa tacaatattc cgcagagatg gagtatcgct catttgtatc
    18241 aatctatttt gaataaagaa gaacatgtga aagaaattaa ttttttgaca acgcttgcag
    18301 gttatgtcca ttggcagctt actggtgaga aagtactagg cgtaggagat gcatcaggca
    18361 tgtttcctat cgatccttca acgaaaacct accatcaaga aatgaaaaat atttttggtc
    18421 agttaatcga aaaagcgcac gtagcgattg acttggatca cattttgcct aaagttctag
    18481 tagcaggaga agaagctggc aagttaagcg aatcaggggc ttatctgatc gaccctacgg
    18541 gaaatttgag accaggaatc ccggtttgtc cgccagaagg ggatgcagga accggtatgg
    18601 tcgctacgaa cagtgtagcc aaagggacag ggaacatttc ggctggtact tccgccttcg
    18661 ctatgatcgt attagaaaaa gaactagaaa atgtccatcc ggaaatcgac atggtcacta
    18721 caccttcagg agaactagta ggcatggttc atacgaataa ctgctcttcg gatatcaatg
    18781 cttgggtcag tatttttgaa gaatttacca attctttggg tatggagata agcagagata
    18841 aattattcac ggtattattc aacaaagcgc tagaaggcga tacggattgc gggggattac
    18901 tttcttatgg ttatttttcc ggtgaaaaca ttacaggagt aaatgaagga aggccattat
    18961 ttgtacgtac accaggtagc cgttttgatt tagcgaattt catgcggatc catttagcca
    19021 gtgcttttgg tgcaatgaga atcggaatgg atatattgaa atctgagaat gtccaaattg
    19081 atcgtttagt ggggcatggg gggattttta aaacaccaga agttggacag cggattctag
    19141 catctgctat ggaggcacca gttacagtga tggacacagc cggagaaggt ggagcgtggg
    19201 gaatcgcgtt attagcagcc tatatgatgg atcgacatgg gaagtcttta gaaaacttct
    19261 tggcagaaga cgtgtttgga caagaccaag gaatcaccat cacaccttca gcagaagaaa
    19321 tagcaggcta tcagatattt atgagacgtt accgtgaggg gatcgacatt gaaaaaacag
    19381 cgattcatca attgcaagaa aaaggagtga tagaaaatgc tggaacaact gaaagaagaa
    19441 gtataccaag caaatcttga cttgcctaaa cacggactcg tcaaatatac atggggaaat
    19501 gtgagcgctt ttgatcctga aacacgatat ttcgttatca aacctagcgg agtcagttat
    19561 gaagagatga cagcagacga catggtcgtt gtggacttgg acaatcatat cattgaaggt
    19621 aaactcaatc cctcttcgga tacgcctact catgctgttt tatatcgttc atttcctcaa
    19681 atcggaggaa tcgtccatac tcactcgaca tgggcgacga tctgggcaca agcaggattg
    19741 gatgttcctg cgatggggac gacgcatgca gatacttttt atggatcggt accttgtgct
    19801 cgatttttga cacaacagga aatcgacagc gggtacgaat atgaaacagg aaaagttatc
    19861 atagaaacat tcaaagaacg caaaattgat cccttagcaa ttccaggggt acttttacac
    19921 ggacacggtc catttacttg ggggaaagat gcgcaaagtg cggtaatgaa cgcagtcgta
    19981 ctagatgaag tatgcaagat gaatttattt accagacagc tcaattcatt ttcagaagag
    20041 ctgcctcaac ggattttaga taaacattat ttacgaaaac acggaaaaaa tgcgtattac
    20101 ggacaaaaca aataaaagga gtggctaatt atgttgaata ttggagaaaa agaattttgg
    20161 tttgttgtag gatcacagca tttatatggg gaagaagcac tgcgagaagt aaagaaacat
    20221 gcacaagaaa tggtcgatga actgaatgaa aatggccaat tgccttatcc aatccgcctt
    20281 caagaattag cagttactgc tgatacgatc acaaaaatca tgaaagaggt caattatcga
    20341 gaagaagtag caggtgtgat cacttggatg catactttct caccagcaaa aatgtggatc
    20401 cgcgggacaa agcttctaca aaaaccattg cttcatttag ctacgcaata taacgaaagc
    20461 attccttgga aaacgatcga tatggatttc atgaatttaa atcaatccgc tcatggtgac
    20521 cgcgaatatg ggttcatcaa tgctcgatta aataaacaaa ataaaatcgt cgttggctac
    20581 tggaaacgac ctgagatcca aaaagagatt gctgattgga tggatgttgc agtagcctat
    20641 aatgaaagtt ttggaataaa agtggctcgt tttggtgaca acatgagaaa cgtgggtgtc
    20701 acagaaggag acaaagtgga agcacagatt caatttggtt ggactgttga ttattttggt
    20761 atcggagatc ttgtacaagt aatcgatcgc gtatcagacg aagaagtcga tcaattattt
    20821 gaagagtaca aggaattgta taccttcgac tatggtgatt atgaagaaaa gacatgggag
    20881 gaacatgtaa aagtacaagc tcagcaggaa atcggtattc gccgtttctt agaagaagga
    20941 ggctataacg cttttacgac taattttgaa gacctgtatg gaatgaaaca actacctgga
    21001 ctagctgtac aacgtttgat ggcagaagga tacggttttg ctggggaagg cgactggaaa
    21061 actgcagcga ttgatcggtt gctgaaaatc atggctcgag ggaaagatac aggattcatg
    21121 gaagattata cttacgagct agctagtggc caagaagcaa tcttggaatc ccatatgatg
    21181 gaagtagatc cgacgcttgc tgcgacgaaa ccaagaatcg ttgtttctcc tttatcaatg
    21241 ggagatagag aagatccggc gcgtcttgta tttgatggga aagcaggaga aggggtagtc
    21301 gtatcgatgg cagatttcgg tacgcattat aaactgctga tcaatgaagt agaagccttt
    21361 gaaccaacga ctgaagcacc taatttacct gttgcacgtg tgttatggaa aactaaaccg
    21421 aatttccatg aaggtgtcca ttcatggatt caggcaggtg gcggtcatca taccgttgtc
    21481 tcgctaaatc taacaacaga tcaaattgaa acgtgggcaa aattagttga gttggaaaca
    21541 gtggttatta gataaaaaga ggttgtgaaa aagccactta gttttaagaa ataagaagag
    21601 attgacgaaa tttgcttttc aacttttttg aaatttagtc ttattatcaa tgtcagctaa
    21661 cggaacacca tttatcaaga tatctcaccc tcaatatttt gctctcgaaa ataaggctta
    21721 tggcagtaac ttgtcatagc cttattttta tcactcagcc tactctgctc taactgctct
    21781 ttttgcttct aggaagtcaa atggtaaaca gttgtctaat tgcagaaatt tcgagatact
    21841 tgttagtaag gagggggttt tatgtatcac gttcatttga ttgatgctat tccagaacaa
    21901 gagatagaaa agttacaagg ggaatatttc aaaataatag atgatttgcc agccgaagga
    21961 atagttattc gtagtacgaa agtgaaagat gaatggctta caccagattt gctagcaatt
    22021 tcaagagctg gtgttggggt gaacacgatc aacgttgaaa aagcttctga aaacgggacg
    22081 atcgtcatga atacaccagg tgtaaatgct aatgcagtaa aagagcttgt tttgtgctgt
    22141 ttgctcttat cttcccgtcc gataatcgaa gcaagtcgta tggtacaaac actaacaggt
    22201 cctaatattt tagaacaggc agaaaacaaa agaagtgctt atgtgggacg agaattgcaa
    22261 ggcaaaacca tcggtcttct tggtcttgga gcgataggca caaaagtagc gctaagttgc
    22321 tatagcttag ggatggatgt cttgggttat tctatccgcg atgcccagtt ggattatgtt
    22381 cggcaagcag atttagaaac agttctttct acctcggatt acatcgtagt catgctgccg
    22441 ttgacagaag atacaaaagg attgatcgat caggcgaata tagaaaaaat gaaaaaagat
    22501 gctgtactta tcaatgtagg aagaagtgaa attgttgata aatatgctgt tatgcaagca
    22561 ttggaaaaac aacatctggc taaatacttg actgattttc cagaagaaga gttcttagaa
    22621 aatgaccgta tacttatgtt gcctcatcta ggggatcgac tcaggaagct ttggcagaca
    22681 gcggacgttt ggcagttgaa gccttaaaag attatctttt gttcggtacg gttagagaag
    22741 cggtcaatta tccatccgca cgaatgtttt tccaagcgcc gtttcgattc actatatttt
    22801 atcaaaagaa aaccaatata ttagcagaga tattttctct gctgaatgag aacgagctag
    22861 cgatcgcaga tatatcgcgt aaccataaga atgagcatgt ctacatattg attgacatag
    22921 acagtactga ttttgaacag ctcaatcaag tgaaacaaca gctagaaaaa atttctggag
    22981 tacgaaaggt ccgcttatta aagaaaccag aaaggccata aagaaagaga atatttggat
    23041 ttcattaatt cttctttccg ttctttactt tcgaatttcg tacaactaaa ataagagaag
    23101 caacattctt ttattgatag aaatgaagga gcaagcattt gaaaagatat ataagcagac
    23161 tttcgaccaa acagaaaaag attttaaaag gaacagcagc aatcatcagt atcttttttc
    23221 ttgttgtttt tagtaattta tttttgcagt ggtgtcaaaa tgatctatcc gtagaccttg
    23281 cgttgaaatt tgctttttct tggcatacgg aaaaattctt cttagcctgt ctcgtcttgc
    23341 tgattattct tatttttctg attgcactag caggatctgt ccctttaggt tcccttacgt
    23401 atgtcgtagc catcggtgta ttaggttttg caaattatat gaaaatgagt tatcggcaag
    23461 aaccgattta tccagacgat ttgaagatga tcactgaaat cggattgctg aaagatatga
    23521 ccggcactat gctgtttaca gtgatcttag ctgccgcagg aaccgtgata ggattgtttt
    23581 gctggtacat gtttcgaagc ctgaaaaaag gaaggcgatt ccaattgatc cgattaacga
    23641 cactgcttgt ggcaattgga ctgctagggt atatcagtaa tttcaacaat cccgataatt
    23701 tgttgcgaaa agcatataat aaaacggcac tatggatacc ttacagccag aaaatgaact
    23761 actataacac tggttttatc ggtgggtttt tgtacaatct gaaggtagaa ccaatggatg
    23821 aacctgaagg atattcgaaa gaaaagatca aagaaatcac tgaaaaatac cagaagctag
    23881 ccgatgaaaa aaataaaact gtagaggaag agtcgccgaa catcgtgttc gtgatgagtg
    23941 aaagtttctc agatcctagt cgattaaacg gcgtagaagt atctggtgag ccattggcag
    24001 attattatga agtagctgat caaacatata gtggcaatat gctttctcaa aactatggcg
    24061 gtggaacagc aaatatcgaa tttgaagcat tgactggttt ttctatggcg ttatttaatg
    24121 cgcaactcac caccccttac acgatgcttg taccaaaaat ggatcagctt ccttctatcg
    24181 tttcagcgtt aaacgcacaa tcataccaga cgacagcgat ccatccatac aatacttcga
    24241 tgtataaaag agaggatgtt tatcagacat taggatttga ccagttcatc agcgagcgta
    24301 cgatgaccta tacggatacg atagagaata atccatatat ttctgatgag tcagcttaca
    24361 aagaagtaat gacacttctc aaggaagaaa aaacacctca gttcattcat ttagtcacga
    24421 tgcagacaca tatgccctac gatggaaagt atgataatct agactatcct gcaaaaactt
    24481 cagatggaag tgggacactt gacttagaga attatctgca agatatttcc tatagcagca
    24541 cggcactgaa gcagttcacg gaagaattga agaatctttc tagaaggact ttagttgttt
    24601 tttgggggga tcatcttcca gggatctatt cagatacgat ccaagcaaaa aatgacaaac
    24661 aaacattgca tgaaacacaa tttcttatgt tcgacagtaa aggagaatta gaaaagcaaa
    24721 cgactcacga tgcaatcacc agtccgtttt attttgcagc gaatctaatg gaacagacga
    24781 atcagacaac aaatggcttt tatcagctgc ttttatcatt agaacaggaa cttccggcgt
    24841 ttgaacgaga actgtactat caaaacggac agtggtataa agaagcgcaa ttcaatcgct
    24901 cgcaacaaga gatttacgac gaatatcaat tgatccaata tgacattgtc gcagggaaac
    24961 aatatagttt agcagatggt ttctttgagc acgaataaga atggcgagct gtatagaaac
    25021 cattaaattt tttcaagtac ttgtcatttg aggataaaaa caagtagaat aaataataac
    25081 aacaatggag aaagtaggaa aacgaacatg ttaccgaata ttggctatta tgtgcagtcc
    25141 atcgatcaaa tcgtcaagga aacagaagca atcggagaag gaatgcatcc gaaatatgaa
    25201 gtgatcagaa acgcaattga tgaaggaaaa acaagtgaac tggacaaatc gactcttgaa
    25261 gagatcatcc tgttatttga agaaggaaca aaaaaatatc gggcaatgct tatgaaagtc
    25321 agtactttgc gtccaccagc aaaagcaatg ggcattcata aaaattttga aaaagcttat
    25381 ctttcatatg tagcaggctg tgaagaaatg atccaaagta tcgatcttga atcaggggta
    25441 gatgaagatc tattcaatac atctgaagca aaacaagacc aagcgacaga ccaattgaca
    25501 gctgccatcc aaaaaatgac taatttgttg ttgaaaaaat aaaagaaatc tttgataaca
    25561 ataaaaacta gagttatcta atataaatgt aatattacct ttatgataga gtgatcaaaa
    25621 tcctttatga taatgacata cctataacca acaacaacct tgtacttact caaaatcgag
    25681 taaatactcc ttttccgtcg agtctaacca tctcggcggt tttttatttg tagaaaagaa
    25741 catcaggata gacggaaaag tcttgtcctg atgttctttt cagatagtat ggaaagctga
    25801 ttagttttct acattctgta catattgttc aaagtagttc tgtaaatcgg ctttgagttt
    25861 ctgatattcc tcttcgctat actctttatc ttcgattttc ccatcaaaga aaggaatatt
    25921 tagttcttcc cgtagtctgt ctaatacgtc ttgctgattc atttcattct ccttttctta
    25981 ctaacgatca aaataaacac agacacattc tggtacatag acatcttttt tcagtaattc
    26041 cagtaatcct gtttcaggat gacggcgata taatgtcaga ttatcggtgt tctgattggc
    26101 acacacgaca aaattattgc ttggatctag tgcgaaatcg cggggaaaat ccccttctgt
    26161 tgagatgatt tgcttgtttt gaatgtggga accatcttca aaaactgcga atacagcaat
    26221 cgagttatgt cctcggttag aagcataaag gaacttgcca tcatttgaaa tgcggatagc
    26281 tgcccctcca ttttcaccgt cgaattcttc tggcaaggtc gatattttct gtaattcttc
    26341 aaaagcacct gtttctttat tataagaaag cacgcggact gtgctgtcta attcaccaaa
    26401 taaataagcg aattgtccat ttggatgaaa gacaagatgg cgagggcctg ttcctggctc
    26461 tgcagtgaag gtagttacaa gattcaattt gccgttttcg gacacatcat aagtatacac
    26521 acgatctgta ccaagatcac atacgactaa tcgttggtca ggagtcagat ccgtatagtg
    26581 gacatgcgca tgatcttggt ttttatgagg acctgtcgct tcttgatgga atattgaatc
    26641 tgctgctttc agttcgccat ctcctaatat atggtagaca tttacttcac ctttatgata
    26701 gttcgccccg tatagtaatt gacgtgcttc atcaaccgct acataacaca aaggagcgcc
    26761 agactctgtt accgcattca aaaaatgaaa atccttatcg tacgcaccgg caccacctaa
    26821 tccatccaca ctagtaactg tgtaaagatt gccagcttta ctttttgcta cataggtagg
    26881 gctattttct tcaacagcta atgaaaggtt ggctaattct tctttttctg tatcaaggtc
    26941 gatcgtatag attcctttac tttcgcgacg cgtataggtt cctaaaatta tcttttcgat
    27001 catatgagct gattcctcct aattgtaatg aaatagtttt cgtttttatt ctatcacata
    27061 attattccac acggctaaag aatgagaatg tgttatactg tttttgaaag caattaaagg
    27121 agagagtgca atgattcagg caaagattac agaaattggg acagaagcaa taaacaagga
    27181 agaacccatg ctgattttgt tcgataaaac agcaacagct gccttacgaa aatattcggt
    27241 gattcaagaa gtatcaaaag acgaaccttt ttctttgaaa gcaggcggaa caattgcttt
    27301 tgatgatcag gtatatacga tcgattatgt cggtccgatg gccaatggga atttaaagac
    27361 agtagctcac gtatcattga tttttgatgc ttgtccagaa gaaggacaaa ttgcaaatgg
    27421 aatctatctt catccgcata gactgcctgc tattggtttg ggaagccaaa ttttttataa
    27481 ctaacaggag gaattatgga aggaaaaaat aagaagaaca gaccggtatc gtggttttgg
    27541 cgctggtttt taaataatca agtagtcaca gccttacttg ttgtattgtt aatattgctg
    27601 atcgtctttt tatttacaaa agtttcatat ttgtttgcac caatctggca atttttagca
    27661 atcgtaggtc tgcctatcat tttagcagga atcttgtatt acttgatgaa tccggtagtt
    27721 gattactttg aaaagcgaaa agtaccaagg ctttacagta tcattgggct gtttgttttg
    27781 gttgttgcac taatcgcatg ggggagcgta gtgatcatcc ctaagattca ggaacaaaca
    27841 gtgagcttca tcagtaactt ccctcaatat gtagatacga ttgataataa attaaccgag
    27901 attttgcggg acccgctgtt caaccaattt cgcgaacagt tggaaacagc aggtgataag
    27961 ttcatgaatt cggcaggcga tatgatccaa gatatatcta agagtacagt tcagagtctt
    28021 ggaagttttg tcggcgcggt agcgactatt ttagtggctc tgctgacgat gccgtttatc
    28081 ttattttatc tattgaaaga cggtaaacaa ttagcacctt attttgtcaa atttttaccg
    28141 acacgcatgc aaaaaccgac gttgaatgtg ctgagtgaaa tgaatagtca agtttcttct
    28201 tatataagag gacaactaac tgtggctttt gctgtggcaa tcatgtttat gattggtttt
    28261 gcggtcattg gtttagagta tgctgtcacg ttggggatta ttgcgggttt tttaaacctg
    28321 atcccttact taggatcatt tttagcgatg attccggcta tctttttagc cattgtagca
    28381 ggtccattca tgatcatcaa agtactgatc gtttttgcag tagagcagac aatagaagga
    28441 cgcttgatct ctccattagt actagggaat cagttagcaa tccatccagt aacgatttta
    28501 ttagttctgc tgacttctgg gaaattgttc ggaatcatcg gtgtgatctt ggggattcct
    28561 gtttatgcag cagctaaagt agtgattact catgtttttg aatggtacac aacaatctct
    28621 tctctttatg aagaaaatcc gttgaaaaag cagaaagaat aacaaaaaga gacagatggg
    28681 aaaatatcca aggcacaaat gctgctttga agattttttc ctctgtctct tttgtatgca
    28741 tttatctcaa tttttaaaat gatttaccta aagcagcagc tttatctaat gcattttgca
    28801 tcaactcatc tgcacggtca gggtcataat cgattccttc gatgaatagc tgatctactt
    28861 gatcgatgcc tacaaagttt aaaatacctt ttacatattg agaggcaaag tcttgtccgt
    28921 tgtatacacc gccattagat tggatgtgca gtgctttttt acctgtcact aatccttctg
    28981 gcccgttttc tgtatagcgg aacgtttttc cagctacatt gacggtatcg aaccaagctt
    29041 ttaattttgt tgggatattt agattccata aggcgtttgc gataacgatt ttatcagcag
    29101 caaggaactg atcagtcaat tcattgaaac gagcaatttt ttgttgttga ctttctgata
    29161 aggaagtgaa ttctgcacca gcacgtaatg cttcccaacc tgataataat tcttcatcga
    29221 tttccggaat atcatcgcga taaagttcca aaacagtcac ttcatcatct ggatgatttt
    29281 ctttataact tgttaaaaaa gacgtcaatg ccttaactgt gcgcgattct tctgcagtta
    29341 ggggatgacc tttgactact aaaacagatg ccatatgctt accttctcct tttattgcta
    29401 tgattgttat aactatgact aacaaaatca gtttactcga aaaagaaaag gctatcaagt
    29461 catattgata aggttgaaga ataaataact aaaaaaagac agggtataaa agaggctgtg
    29521 acacgatttt ttgtcacagc ctcttttgaa tctaacaagc aatattcaag caacactcga
    29581 ctgaagagct tccatcccac caatgatata aacagcacga aaaccattag cagtaagttg
    29641 atcggctaat gcttctgatc gcctgcctgt atagcttaga atataataaa gtttctttgg
    29701 atctagattt tttaattgtc ttggcaagat agcagcgggg atagaaagcg agtggcttaa
    29761 gtggtttttt gtgtaatcta aggtatttct tgtatctaaa acgatgctgt cagcatcgag
    29821 aggcaattta ttgaagtctt ggacactaat tgattgggcc atttatctca tcctttgttt
    29881 agtatgtggc tattttaatg ggcaactctc attaattcaa atggaaggat tcctcttatc
    29941 acttttttaa tgtatcggat gataatgaaa aaaactatgc aaatgtcttg caaaacaggt
    30001 aggaggtgtt ataatgacat agcgtcatat gacaatgtgt catcttgaaa gaaaggagaa
    30061 tccatatgcc gacacagaca tttttccatt tgcctaaaga aaaacaaaaa agactaatcg
    30121 aggctgcgag aatcgagttt tctagagtac ccttaaaaga agcttcgatt gctaatatcg
    30181 tcaaattagc ggatatccct agaggcagtt tctatcagta ttttgaagat aaagaggatc
    30241 tttattttta ttactttgag acaatgagac gtgacagctc taaagatatg ttacaactaa
    30301 tgaaagaatc cgatggtgat ctattcaaag gattcgaatc ctatttttct aaaatgattt
    30361 atgagatttt ggaaggcgag aatgcttctt tttatcgtaa cttgtttatg aacatggatt
    30421 accgtgcctc aagaaaagtc gctcctcata cgagtcgcca aaaaacgcct cacgatccac
    30481 ataaagaaca caaacaagat gcaaaagagt tgcttgaatt gatcgatcgg acgaagctga
    30541 aagtaaaaaa tgatcatgaa ttggaattac tgatccaatt agtgatgaat actgtattta
    30601 caacaattgc gcattcgtat cgcttatttt caacatctga agattactca gtagagcaag
    30661 caatcagtga attcaatttg aaactttctt ggctgaaaaa cggagtatat aaatagaaag
    30721 aagggaacta gatgctaaaa atcgtaaaac ggatctcctt gacttcagca attgcagctg
    30781 cagttttcat ggtgatccaa gtactggcag atttatattt gccgacactt acctccaata
    30841 ttattgataa aggggtagcg caaggagatg tcgattatat ttggcataca ggttttgtga
    30901 tgatcggctt ttctttgatc agtatcttag ctgctatcgc aaatacattt tttgctactc
    30961 gtgaatcaca gaaactgggt aagcaattgc gtacggatgt ctataaaaaa tcagagagtc
    31021 tgacaaaaga tgcgtttgat aaatatggaa cggcctcttt gatcacaaga acgaccaatg
    31081 atgtgactca aatccaaatg gttacacaaa tgtttttacg tatgatgatc aatgcaccaa
    31141 tcacgttgat tggcgcgagc atacttgctt atcaaaaaga tcatcagttg acaaaaatct
    31201 tcttagttgt catcccagta atgattattt tgatcggcgg aatcatgtac tttgctgttc
    31261 cattattcaa aagtatgcaa aagaaaactg accgtttaaa cctggtattt cgagaaggat
    31321 tgacaggggt cagagtgatt cgagcattcg ataagacacg ctttgaagaa aatcgttttg
    31381 atttagcgaa taaagattat acaaatacgg caatcaaagt taatacgatc gtcgcattga
    31441 tgatgccaat gatgacattg atcatgagcg gtacaaacgt agcaatcact tggtttggtg
    31501 gacactacat agcagatatg actttggaag tcggtaatct gattgcattt atgacttatg
    31561 cgatgcagat cttgatcagc tttatgatgc tttccgctat ttttatcatg gtgcctcgtg
    31621 cacaagcttc agctgaccgt attaatgaag tgttggatga aaaaatcggt atccatgacc
    31681 ctgaaaatcc taaaacagtt tcttttgctg gaaaaaacgc aacattggcc ttcaatcatg
    31741 tcaattatcg atatcatgga gcagaaaaac ttgcattgga agatattgat ttccaagcca
    31801 agtcgggcga gatcgtcgca atcatagggg ggactggttc aggtaaaacg accttagtca
    31861 atttgatccc tcgattatat gacatcgaat ctggttcgat ccaaataaat ggaacggaca
    31921 ttagtgacat gactcaatat aatttgcgag aattaatggg ctttgttcct caaaaagcag
    31981 ttctgttctc tggaacgatc cgagacaata tgcagtatgg taagccggat gcaacagatg
    32041 aaatgatttg gaaagcatta gaaatcgcac aagctaaaga ttttgtttca gagatggaag
    32101 atggcttaga tagccatgtg gaacaaggag gaggaaactt ctctggtgga caaagacagc
    32161 gtctggcaat tgcacgggca ttagtcaaaa cagcagatat ctatgtgttt gatgattcct
    32221 tctcagcgtt ggatttcaaa acggatgcca atcttcgcca agcattgaaa acgaatatga
    32281 aagaaagtat caccgtttta gttgctcaac gagtaagtac agtaatggat gcagatatga
    32341 tccttgtgct agatgaagga aaactggttg gcaaaggaac acatgaagaa ctattagcaa
    32401 caaacgaaac ttatcaagaa atcgttcatt cacaactaag agaggaggac ttagcatgag
    32461 taaaacaaac acacgtccct ctcatggacc agcaggcggt ccaggacatg gtggacctgg
    32521 cagaaatctc ggtgtaaaag gtgaaaagcc taaaaatttc tggggaacgg tacgccgatt
    32581 gtttggctat atgtctaaac gtatggttgc gatcatcgct gtatttgtac tagcaatagc
    32641 agccgtgatc ttccaaatcc aaacaccaaa ggtcttgggg aaagcaacca cagaaatatt
    32701 caaaggtgta atggctggcg ctcaacagat gcaacaaggc caacaagtaa caaaatttcc
    32761 gatagacttt gataaaatcg cgcagatcat tgcgacagta atcgtgatgt atttgatttc
    32821 tgctgtattc aactttttac agcaatttat tatgacacgt gtttcccaac gaacagttta
    32881 tgaattacgt catgatttgg aagccaaaat gaatcgtgta ccgatttcct attatgatac
    32941 acatacaaat ggggatatca tgtctcgagc aatcaatgat atggataaca tcgcaagcac
    33001 gctgcagcaa aatttgactc aatttgtaac tagtgcagtc acattgattg gggtcatcgt
    33061 gatgatgctt tcaatcagct ggcaattgac gttagtcgcg ttattgatga tccctttaag
    33121 cttgatcatc gtgatgatcg tagcgccgaa atcacaagtg tttttcgcag atcagcaaaa
    33181 aagtttaggt ttattgaata accaagtaga agaaacatat ggtggtcata ccattgttaa
    33241 aacattcaat catactgaaa aagaccagga agtatttgaa aaagaaaatc agaatctgta
    33301 tgccgctggt tggaaagctc aatttatttc ggcaatcatc atgccgttga tgaatttcgt
    33361 taaaaatcta ggctatgtgt tcgtcgctgt actaggcgga gtaaaagttg caaatgggaa
    33421 catgacatta ggggatgttc aagcgttctt gcaatataca acacagttct ctcaaccaat
    33481 cactcaactt gcaagtttga tgaacactat ccaatcaaca gttgcttcag ccgaacgggt
    33541 atttgaagtg ttggatgaag aagagatgtc caatgaaaaa tctggccttc cagaagaaaa
    33601 agatacacca tacaaagttc gttttgaaaa tgttcaattc ggctacacac cagataactt
    33661 gctaatgact gattttaact tggatgtcaa acctggtgag atggtcgcta ttgtaggtcc
    33721 gactggtgca gggaagacga cactgatcaa ccttcttgaa cggttctacg atgtttctgg
    33781 aggtagtatt cgttacgatg gtgtagatac ccgtgatctt tcacgtgatg aattgcgcgc
    33841 acagttttca atggtactgc aagatacttg gctgtttaca ggaagtatct atgacaatat
    33901 caaatatggg aacgacgatg caacagacga acaaatcatt gaggctgcta aagctgctca
    33961 cgtagacgat tttgttcgta aattgccaga aggatataac actgtattaa acgaggatgc
    34021 aagcaatatt tcgcaagggc agcgtcagct aatcacaatt gcgcgggcat tcttagcaaa
    34081 tccagatgtc ctgatccttg atgaagcaac atctagtgta gatacacgaa cagaaatact
    34141 gattcaaaaa gcgatgaacc gcctattgga aaaccggaca agtttcgttg ttgctcaccg
    34201 cttgtctaca atacgcgatg cagacaacat tattgtgatg aatcatggtt cgatcgtaga
    34261 aacaggaaac cacgatacat tgatggaaaa agacggattc tatgcagatc tttataacag
    34321 tcagttttct gaagaagaag cttcatagaa tagtacaaat ttattatttt ttttaggggc
    34381 tgtgacaaaa gtcgtgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgtcc
    34441 ttcggtatta gctcaaaaaa cggcaaaata tgagaagcta tttttccgtt ttgtatctct
    34501 aatactcagg tcaaaccgct gattttacaa cctcttcctt aaacggtgtt ctcctgccaa
    34561 ctcctcggtc acaagtcaca atagtcaaca aacctgagaa gctgtttttt gaaattgctc
    34621 cttgtgactc ggagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
    34681 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
    34741 ttcttactac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
    34801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
    34861 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctttttat aagagttttt
    34921 actcattaat ttacaaaaaa caagcaaaat gcgaattttc tcacattttt tttaacaaaa
    34981 tcaaagttat tgcttgaaag aagaagaatc catcgttaaa ataaagcttg acgtagaggg
    35041 gagcaagaaa tatgaaagat tttcaattgg gggctgccac ctatttaaaa agtgtagcca
    35101 ttcgggtgaa agaccgagat aaaatgattg atttttacaa gaacgttgtt ggctttgatt
    35161 tgaaaagaga agaaaacgaa ttagccattt taggaacaga agaagctgga agtgaaatgt
    35221 tgctgctgga agaaagtcct agagcgaatg atcacatggg ggtgatcaaa aagctgaacc
    35281 gtttttcatt gattatccct agcacagaag aaatggcaga tatcctttgt cgtatccgta
    35341 aaatgaatta tccaatcaaa gaagctttag aagatcatgg acgtatgggg attttgctcg
    35401 tagacccaga agagaatgaa ttggaaattt accatgaaga gaataagaac gaccaaagag
    35461 aaattccaga aacaatggat caagaagcat tattaagaaa atctgaagaa aaattcagta
    35521 aactatcaac aggttcttat tttgaaaaaa tccatttaaa tgttactaac ttaacaaaag
    35581 aacgccaatt tcttaatcat gtacttggtt tgaaggtaca taatgaagaa gagggcatcc
    35641 atgtattgaa tgaaggagat ttccatgttg gtttgacaga agctagcgga ggcgcaattg
    35701 atttgcctac agatgaagtg attggacttg attttctgaa attcatgatc gatactgaaa
    35761 gcatggaagg actgattgct cacttgcagc aactatcagt tgatttcttc ttagacaaaa
    35821 agcacacagt actcacaatc tatgatgcta taggaatcga atggtggttc gtcagtgagc
    35881 caaaagaaaa gaataaaact gacattcgat gcgacgctta actttttctt tcttaacgat
    35941 tgtcgcatgt ctcgtcattt tatgaacggt catactatcg cttattctag agatagaaag
    36001 agaaaatagt ataccggggg aaactgcgcg gtttttcgcg cagttttttt gtgtaaaaaa
    36061 ggttgattcc tcatagatta agctttaagc ttggactgta agaatctgat agaaaagcag
    36121 gagaaaaaga tgtatccaca cacgaaacag cttattttga aaaagatgga agaaggattt
    36181 tatccaggtg tagcagtcgc cttcataaaa gaaaatcaag tagaaacatt tacagccggc
    36241 tttgcccaag tcgttcctga aaagcagctg atgacagaag atctgttgtt tgatgcagcc
    36301 tcactgacga aagtcgttgc aaccacttca ttagttttac gtctattaga agatggaaag
    36361 atagaactcg atcaaccgct tcataaatat cttcctgaat ttgaaaatca gacaatcact
    36421 ttgcgtcatt tactgacaca tacttctgat atcaaaacat ggataccaaa cagagataag
    36481 ctatctcagg aagaattgat cgccgcttat ttgaagctag aaccaggtga gcaactagga
    36541 aaagaagtaa aatatacaga tgccggtacg attcttcttg ggttgatgct agaggaaatt
    36601 taccaagaaa ctgcagtgga aatctttcaa gaacaagtat tggaaccatt aggaatgatg
    36661 aacagcttat ttcttccttt tccttccaaa aaaatcgttc ccacagaaca actgccttcg
    36721 ggtgaggttt tgcgtggaca aactcatgat ccgaaagcaa gagttttggc tgaacatgca
    36781 ggtaacgcgg gtctctttat tgatttgaaa gacagcatta cttttgtcca aatgtatttg
    36841 aacaagggaa aaatccgtaa tggtcgtttt ttagaagaag agacgattga ttctttactg
    36901 caagatcaaa caccgaaaaa aaatggctat cgttctttag gatgggactt aaaaaaggat
    36961 gtagaagacg gtcatgcgat tctttttcat actggttata caggtacgtt tttcttgatt
    37021 gatataaaaa cacaagaagc gttgatattt ttgtcaaatc gcgtacatcc tgtcgatcat
    37081 cgtgcggagt atctcatatg tcgagatgaa attgttgaaa actttttgaa agagaaagcg
    37141 gtattaccga aataatgcta taatagctat gaaaaatgag aggagtttga attatgcagg
    37201 aacctatgtt tttaaaacca gtttttcaag aaaaaatttg gggaggaagt cgtttacgtt
    37261 cagtctttgg ttttgatatc cctaacgata aaatcggaga agattgggcg atcagtgcgc
    37321 atcctcatgg tgtcagtgta gtagaaaatg gcgaattcaa agggaaaaga ttagatgaac
    37381 tatggtcaga acacaaggaa ttgtttggcc atccttcaga accagtgttt ccattattga
    37441 tcaagatctt agacgcagaa gatgatctat ctgtacaagt acatccagat gatgcctatg
    37501 ggatgaaaca tgaaggtgaa ttaggaaaaa ctgaatgctg gtatatcatc gatgctgaac
    37561 ctggtgcaga aatcatctat ggtcatcatg caaagacaag agaagaacta gctgagatga
    37621 tcaaagatgg gcgctgggat gaccttttga agaaagtacc agttaaaaaa ggtgatttct
    37681 tttatgtacc aagcggaacg atccatgcta ttggtaaagg aatcatgatt ttagaaacac
    37741 agcaaagttc tgacacgact taccgcgtgt atgactacga cagaaaagat gcgcaaggac
    37801 aaacaagaga gctgcatatc caacaatcga tcgatgtaac aactgtccct gcgaaaacac
    37861 cagaattaca aatcaaagaa gtccgaaaag ggaattcatc gatcgtcact tatttagaaa
    37921 ctgagttttt caatgtatat gagtgggata ttaaaggaat cacatctttc aaaaaacaag
    37981 ccccttatac attgatgacg gtaatcgaag gagcaggaga ccttgtcgtt gacggaaaaa
    38041 catatccatt agaaaaagga acaagctgta ttattccaaa tggcgtttct gagtgggcgg
    38101 tccaaggtga actagcaatc attgcgtctg aaccaggcaa atagttccca gatagctgaa
    38161 agcagcagac ggtttttcgt ttgctgcttt ttctacataa aatccgtcca tcaataaata
    38221 gaagtctggt gaaaaagatg gaatacaaat ggaaagcaaa caacagcagt cgcgtgtcta
    38281 tgaaacaact gagaaaagct ggacatcact taaatgaaat gatcatgcca aatctaagtc
    38341 tgtttatcgc ttggggaatc ttgtcattag caacaaaagg tgtgtctggc gatttgcaca
    38401 ccacattaga agaggtatgc cattggatga tccagctgct tttacccatc ttgatcagtt
    38461 attcaggcgg gaaatcggta gggggacaaa aaggggcagt cgccggtgca attgcttctt
    38521 tagggttgat cgctcgttcc aatgctccac aaatcgtagg agcgatgatt atcgggccgc
    38581 ttgccggcat cgtatatgaa caatttgttt ctagattcca tgtgaaattc aaggctggat
    38641 atgaaatgct tgtcagcaac ctactagtag gactgactgg tagcgttatt tgtataattg
    38701 gaatcgtttt tattgaacca gttttaaata gtgttcactt gttgctcgcc tccgttgtgt
    38761 cttggctagt cacaatgaat ctgttaccac ttgtcagtct gatcttagaa cctctaaaag
    38821 tactattttt caataatgca atcaatcatg gtgtattgac acctttagga atcgaattct
    38881 cacaaacagt cgggtactct tccttatttt tgatggaggc aaatcctgga cctggtttag
    38941 ggatcttact ttctatcctt tgttttgcag aaaaacagga aaaagtaaat gcgagtggag
    39001 cattgatgat ccatgcgatt ggcgggattc acgaaatcta ttttccattt gtcttgctgc
    39061 gaccgtatct atttttagct gtaatggctg gtggggcaag cggcacgttg atatttcaat
    39121 ggttagatac gggattagct gcacctgtat cgcctgggtc actactcgtg atttttgcca
    39181 atgcgcctta tccacaacta gctggggtaa gtatagggat agcaatcagt acatttgtga
    39241 ccttcatttg tgcagcgata ctattgaaat taccaaataa agaaaaagag aacaaaacgg
    39301 tcaaagaaag gggagaaatc ttggaaccat ctatcattca ttccgtcatt tttgcttgcg
    39361 actcaggtat gggatcaagt gctatgggag ccgccctgct gaaaaagaaa ttagcagcag
    39421 aaaaaatcga tctttctgtc ggatacagct ctatctatga attgaataac caagtaaatc
    39481 agctggttat cgtgcaacat gaactagcgg gtcttgcaag agaaaaagta cctcaaagtc
    39541 aaatctatgt actggatcac tttttggaaa cagatatctt gctgtcacga ataaaagaag
    39601 cgatccaatt tccaaagcgg gaagaaaaaa aagagatagc agatatcaca gaacaaaaaa
    39661 cgtcgcctgc taatattgta tttttgtata aaaacaacag acgcggctca caaactatgg
    39721 gcatgacgat tttaagtcaa ttagcaaaaa aagcgaaaaa acagtttgtt atctcaaaag
    39781 aatcaataga aaaaatcaaa atccaagaag aaacgattta tatcgcacaa agagaactag
    39841 ctgtaactga acagctagaa gaaaagatcc ctcggctagt tctagttgaa cattgggtag
    39901 ctacgaatga atacgaaaaa ctcgtgaagg gagaaatata agatgtattt atcgcccaga
    39961 gaaaaacaat tattagcaga atttctcagt agcccgtcac ctgtatctat ccaaaaaatg
    40021 atgaatttat tgaaggtcag taaacggact atttaccgcg aattagataa tcttgaagtc
    40081 tcattgaaat cggtaaatgc ttctttgaaa aaagcagcta gaggctcatt tgtcattgag
    40141 gcatcagaag aagtattaga gcaattaaaa gaaaatacag atgggaaaag taatgactta
    40201 tccactgcta aacgacaaca cgcgatttta gccgagttgt tactgacgac agagccgttg
    40261 agtcttgctc atttcatgga atcatatcgc atcagtaata ccacttttta tgcagatatc
    40321 aaacagttgg aagaaagtat tcgccaactg cctttggaaa tcattcggaa ccagggatac
    40381 gagatcgaag gaccggaaaa ataccgccgg cttctgacag cgaatgtatt agaattagaa
    40441 atcaatgaat atgaactttt tcatagtatt tctttcgatg catcactgaa ttattttttt
    40501 cgattcgttg atccgcaaca tctatctctt gcacgcaaag taatcgggga agaattgatc
    40561 caaaaaaaga ccaatctaag tgatagaaag cttgaacatt tggttttgat gctgacattg
    40621 acgatggagc gtgtgacgaa aaatcattta ctgacaaatg agacatataa tagtctggca
    40681 aacaaagaat tactacagac agctaaacga ttgttttcac ggatcgcttc tgagacaaaa
    40741 caactttatc cagtaaatga gatcgtcttt tttgcaggat tattaaatga cttcacgaac
    40801 tcatttgatc agaatttctt tgaagagacg ttcgatacac agcttgctta tttagtaaaa
    40861 caactgattc aagaagtaag tgaagagacg aatgtccatt tttatgaaga tgaaactttg
    40921 tacaaaatgc tgttgactca tttatctggt gtgttttcaa gagccgtttt acaagaagag
    40981 acactgacca atccgatttt agaaaaaatc atggttcaat acgaggaagt tgcgaatgca
    41041 atccgtaaag cagtagagcg gatttttcaa gaaaaaaaat tatccgaaga agagatcgct
    41101 tatatggtcc ttcattttgc gaattcattg gaacgaagtc cgaaagaaag caatatcagt
    41161 attgcaggat tttcgccaag tggattagct tctactagca tgttggaact acgattgaaa
    41221 aggtattttc ccttcatcca acaaattcat ttctatcgga ttgctgattt aaaaaaaatc
    41281 gatttacaag aagaatatga tgttgttatt tctacatcta ttttaccagg atacggtgga
    41341 aaatatattt tagtctcccc gctactgcta gaagatgaag tcaagcagct aaaagaagct
    41401 ttttcgcata ttgagaagaa aaaaaggcaa aaaattacgt ataacagcaa taacgagtgg
    41461 atgatagaag atacttatga ggaaacaatg catttcatgg atcaaatcaa tcaattactc
    41521 ggtcgttttt ttatccaaga actggataat cctgagacga tttccgaaac gattcgcagc
    41581 ttatttcctt attttcctga aggactagta acggatgaag aggtggttca tcgccggctg
    41641 cttaagcgat atgaacaagc acctatcggt attcctcata caaatatggg gttattccat
    41701 acatccagct cattgatttc taaaccgatc ttttgtattt tcaatttgaa acatccgttg
    41761 accattgaag ggatggatag acagccagtc gatttgtccc gtatgttagt tatgctggcg
    41821 ccttcgccaa tccaagaaaa agacgggatt cttttaggaa agatcagtgg agcaatcatc
    41881 atgaatgact tgaacaccga gatcttccgc tcaggaaata aagaaatcgt ttatcagttg
    41941 ttagcgaaaa tcctgattga agaaataaag aaatgaaggt gaaaagatga tacagctacc
    42001 agcagaaaaa atattgctcg atcaatccta tcaaaattgg aaagaagcgt tgaccgagat
    42061 cggccagtgg atgacccaac aagggggaat caaaccggca tatatccaat caatgatcga
    42121 aagacaagaa aaagcgagtg tctatatcgg gaatttcgta gccttgccac atgggactga
    42181 caaagatgaa gaagtagtag aagaaggaat ctatttagtc caagtgcctg atggtgtgga
    42241 ctttggaaca aaagaggaac caaaaatcgc gacactctta tttgtagtgg ctttaaaaaa
    42301 agaacatcag ttagcggtgc tccaagattt agcctttttc tgttcagaca tcgaccaagt
    42361 aatggcgctc agtgatgcgg aaaccattga agaagtccaa atgatttttg aacatggaca
    42421 tatataacca atgttcacag taaaaaaagc tgccttgcat caagtaataa cttcaaccaa
    42481 gataaaaata agaggagcta tttttgttgg gaggttctta ttactgcgga tgcaagcttt
    42541 tgaaaactat ttattctaga aaaccgttta tttgaaatag gggacggtga caaggttttt
    42601 gtcaccgccc ctattatttt tgtgttttaa tagctctttt ttatatagat agtgcccata
    42661 cctatgaata ataaaacgat actccatata ccaagtgtca agataccatt catgaaagta
    42721 acctgttctg aagctagtgg cgagaaaata tctcctccta tgatcacatt ttgaaaatca
    42781 acataagaag ttggcaagta atgactgatt gattctatcc atccaatcga agaaaacaag
    42841 cgatactctg ctaagaaaag acaaagaagt gtacctaaaa gattgactag agaatgttca
    42901 aataaagcag atagaagaaa actgattgta cttaaaaaga gcacccacaa caacaggaac
    42961 agaaagctct ggaaaagata ctcatacatc ggcaggattc gtatctcctg accaatgatc
    43021 gttcctacag gataacggaa gtctcccaaa ccatttttga ttattactag tatagagacg
    43081 atcaaaaatg gcatcacgag acttagcata gctgcaccat aataaatggt aaaacggatg
    43141 ctatgtttct ttaccatact atccggtaat agattgacta gagagattac ctgctttctt
    43201 ttttcataag agatgatgga agaaagaaac aaggcggtta tacataaaat caaaaaacta
    43261 gaaatcattc ctgaaaaaat attttcgtag taatttgcta aagagagatg cgctggcaag
    43321 tcaaaaatag aatatctttg gatctgatgt tctttcatat aacggagcaa ctcgacacgt
    43381 ttttgttgtt caatgattgg aattccttgt ctttgaccag aaatcagtcg atccagtata
    43441 tctttttcat aatgataagt tgcttcgacc atttgctgga cattccctgt gttttttgca
    43501 tgtaagattg cttcgatcag tttgttgctt ttctctaaac tttccacaaa ttctttctca
    43561 ttctcatctt ctttcaacgt ttcaatcaaa cgttgcatct cttcatagtt atgattcagt
    43621 tcatcgaatt ctgtataagc aagtttatga tcaaaaaaaa gtgggaatgc aaagagcacg
    43681 accaacaata gaggaaataa aaagtttact ttgtttttta gtaagagatt gcgttcaaat
    43741 gtaagtaaag acatagttac acctcatata atttacgata aatagctaaa atctcttcca
    43801 tatttgagga ttctgtgacg atttctccat tttttagaaa acatgcttta gaacaaatcg
    43861 attggatatt cgcgatagcg tgggaagaaa gaagaatcgt cttcccctct ttctgccatt
    43921 gtctgatgag gctattcaag atatcaatac ttcctgggtc taatccgttc aacggttcat
    43981 cgaataacat gacagatgaa tcactgatca agtaagcagc aagcaagaca tgttgtttca
    44041 ttcctagaga catttttcca atcggcaaag taaggtactc agtcatcttc aatcgttgga
    44101 aaacagtttg gcattctact ttgctctgcc aatagtattt gatcaaagtc aaataatcaa
    44161 cagccgatag atagtcggat aactgattcg ttgattctag aaaaaagaaa gaccggcgat
    44221 aatctgtata agcagtagga gagatgttgt gaagggacaa ttgatcataa gttgttggca
    44281 gcagttgggc aatgccattc agcaaagttg ttttgccaaa tccgttcggg gcaatcaaag
    44341 cagctacttc tcctgattct actaaaagat tcaattgtcg aatcacacgt tttttaccac
    44401 gtttcatgtt catgtttttg atcagtaatg tcatagatag gcctccaata taaaatttaa
    44461 taacataata gaatggaaaa catctaaatt aaatgctaac aaaagttatt gtttgcatat
    44521 tataaaaaaa ttaaaataac agttttattt aaaattgaag ttttctgata ccgaaatctt
    44581 tttattttgc tgacatactc aaacctttgg cacaaagttt cgtgccaaag tacgactatt
    44641 tctcttttta gtaagcggat acaattagag tatcaaaagg agagagggag agacaaaatg
    44701 gaacggacga ttcccaaaaa caacatttcg ctaaaagcga gggtacaaaa attaggcagt
    44761 actttatcaa gcatggtcat gccaaatatt ggagcactga ttgcctgggg tgtattaacg
    44821 gctctattta tcccagatgg ttatttacca aatgaggctt tcgctaccat ggtaagccca
    44881 atgctaactt atctgattcc tttattgatc ggttatactg gcggaaaagt aattgctggt
    44941 gaccgcggat cagttgttgg ggcaattgca acgatgggtg tgattgtcgg aacagatatt
    45001 ccaatgatgc taggcgcgat gatcatggga cctcttggtg gatatgccat caagaaattt
    45061 gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa tgctagtcaa taatttttca
    45121 gcaggtctta tcggttttgg attagcgtta ttaggttttt ctgcgattgg tccagttgtc
    45181 gatgcattga ctcaagcaat ggcaaaaggt gtagagatca ttttatctgc ccatttgatt
    45241 ccattgacaa gtatttttat tgaaccagca aaaatcttat ttttgaataa tgcaatcaat
    45301 catgggattt tgacaccatt gggaactgaa caagtagcag aggctggaaa atctattcta
    45361 ttcttgcttg aagcaaatcc tggtccagga cttggcgtat tacttgcttt tactttgttt
    45421 ggcaaaggag cagcaaaatc ttcagcacca ggagccatga tcattcactt tttaggcggg
    45481 atccatgaaa tctattttcc ttacgttatg atgaaaccgt tattattctt atcagttata
    45541 ggcggaggaa tgtcaggaag tttcattttc caattgatgg atgctggact aagagcgcca
    45601 gcttctccag ggtcgatcat tgcgattcta gcgatgacac cgttcaattc ttaccttcct
    45661 gttattttgg gcgtgattgt tgccgcagct gtttcgtttg gtatttctgc agtaattttg
    45721 aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat ctgttcaaga gacaaaggca
    45781 gctaagcaag cttccaaagg tgcccaaggt attcctcaag gagctacatt ctctggcgta
    45841 caaaaaatca tttttgcttg tgatgcagga atgggatcaa gtgcgatggg cgcttcgatc
    45901 ttgagaaaaa aagtacagga agcagacttg cctcaaacgg tgaccaatca agcaatcagc
    45961 caactgacag atgactcgaa tacattgatc gttactcagg tcgaactaca agaaagagca
    46021 aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa actttttgaa ttcacctcga
    46081 tatgatgaaa tcattcaaga attaagcagc aagcagaaaa catcagtttc tgaaattgag
    46141 aatgagccag cagaagagtt tctttcagca aacatcaaac aaatcaaaga gattctactt
    46201 gtccacgatg atcgttccgg atcagcaaca atggggatga ccgtgctcaa agatatatta
    46261 gacaagaacc atttaactat cccgttaaga aaagtcaata tccaagattt aaaagatgaa
    46321 cctgattcac tgatcgtagc caaagaaagt ttagcggaaa aagtacagat ggctgcacct
    46381 caatccattc atttatcagt caacagcatg gtcaatccgc aaaaatacga atcgattgca
    46441 caatatttga aacagacagc ttaagggagg aaaacagcat gcaaagtttg ccattcgaca
    46501 tgatcgagct caatcgatct tttaaaacca aagaagaagc gatccgctat tgcggtcaga
    46561 tgcttgtaga agcagggtgc gttgatgagg aatatatcga tgccatggta gatagagaca
    46621 aaatgttatc tgtttatatg gggaatttta tcgcgattcc tcatggaaca gatgaagcaa
    46681 aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt accagatggt gtagattttg
    46741 gtaccgaaga agaagaaaag attgcgactg ttttgttcgg tattgcagga gtcggagatg
    46801 agcatttgca acttgtacag caaatcgcac tttattgcag tgatatggac aatgttgtcc
    46861 agctagcaga cgcgctcagc aaagaagaag taacggaaaa tctagcaatt gcttaaggag
    46921 agagaaaaat gagagcagta cattttggtg caggaaatat cggtcgagga tttattggag
    46981 aggttttggc agctaatgga tttgagatta ctttcgtaga tgtgaatcac acgatcattg
    47041 atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc agatgaatca aaagaaaaaa
    47101 tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa tccagaaaaa gtaatccaag
    47161 aaatcgctga agcagattta gttactaccg ccattggccc gaatatctta ccttttattg
    47221 ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga aggaagcaca acatctttag
    47281 atatcattgc ctgtgaaaat atgattggcg gttcagcatt tctagaaaaa gaagtgtata
    47341 aatatcttcc ggaaacatca tttactgaca aatatatcgg ctttccaaat gcagcagtcg
    47401 atcgaatcgt cccattacaa caccatgaag atcctttatt cgtccaagtg gaacctttca
    47461 aagaatgggt cattgacgat agccaaagaa aaaataaaaa aatccaatta aaaggggttt
    47521 tatatgtaga tgatctagaa ccatatatcg aacgcaaact atttagtgtc aatactggtc
    47581 atgcgactgt cgcttataca ggtgccttgt taggttacca gaccattgat gaagcaatgc
    47641 aagatgcgtt agttgtggca caattaaaat cagttttgca ggaaacagga agtttgctga
    47701 ttgccaaatg ggggtttgat gcagagcagc atcatgcgta tattgaaaaa atcattcatc
    47761 gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt cgctcggaca ccattacgta
    47821 agttaggtta tcaagaaaga tttacacgtc cagtgagaga actacaagaa cataacttga
    47881 cttgcccaca tttgacggca acaatgggga ttatttttaa ttactatgat cctgaagatg
    47941 aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa cttggaacaa ttgattcaag
    48001 aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat caagcaaaac gtgaaccgtt
    48061 atgccaagca agtcgcttaa aataggatac gaaaaagggg aatgtgacga aatttttgtc
    48121 acattcccct ttttcgaata actaggctgt ttgtccttca agatagggca gatccaattc
    48181 tttaccgtaa cgttgttcta aagctaggcg gatcaaacga gctgccaggt tctcatttct
    48241 tgcaaggata ggtccatgga aataagaacc gaagacattt cggtaaatga caccttcaga
    48301 ttggtcttca ccattatttc cttgaccttg taccactttc cccaaaggtc gttcaccttc
    48361 acctaagaaa gtccgaccat tgtggttttc aaatccataa taagtttcat tgaattcttc
    48421 gttatggata acgatatcac cgataaaccg gttattttcc tgacttaacg tgtagtgatc
    48481 cagtgcactg attccttgga tcttttctcc attagctccc atgtagtagt gacctagcaa
    48541 ttggtagcca ccacaaatgg ctaacatgac gccatcattt tcgatatagt tgatcaaaga
    48601 atctttcttt ttctggatgt cttttgaaat gattaattgc tcaaagtctt gaccaccgcc
    48661 aaagaaaacc aagtcatatt tatcagcatc aaaaggttca tgtatactaa cgatctccgt
    48721 atggcaagaa acccccattt tttctgctat atatttcaac atcaaaaggt ttccgttatc
    48781 accatatgtg ttcatcaaat ttccataaag gtgggcgata ttgagttcat aattagccac
    48841 ggttcattcc tcctttgata aatccttgag cagctagttc tttacgaagc tgcaatacag
    48901 cagtgtaagt agctaaaata tagacatgct cagttggcaa ttctttgatt ttttcgatta
    48961 cttctggtaa ttcttttgtt tctgtcaatt tattttctgg aatcccggca acttttagac
    49021 gtaaagtcat atctgtatga cggtctcctc cagaaataac agctgggata tccatatcgg
    49081 caaagttttc aaaattacca tcccaaatcc agctgacatc gattccgtct gcgtaattgg
    49141 catttaataa agccactaaa gaaaatggat aaggagctag accaatcata tcgattactt
    49201 gattgatacc tacaggattt ttgacaagta ctaatgtaca ttttttccca ttgatattga
    49261 tgacttcttg tcggccgaat actttctcgt catatcctaa accttgtttg attttatcag
    49321 gatcgacatt gaagtactct gccactgcag tagctgctaa agcattgtag acattataca
    49381 taccgccaac ttcgattccg tattcatgac catcgatcac gaaatctgct gacacattgt
    49441 ccatccgtac catatcagtt aatcgataat ccaattcagg acgtttgaag tcacaatttg
    49501 gacagtaata tttccctaag tttgcataag tgatcatttt ataatgcaag atgtgatgac
    49561 aatttggaca taagacaccg tctgtattgt aatgagccat ttgttcacga tcttcttcat
    49621 ggtcaaagcc ataatatttt ctaggattga ctgtttcgat agaattgaag attggtgaat
    49681 caccgttact gataatcgtt gcatttggtg actttgcggc accgtctaca atcattttat
    49741 aggtcgtata gatttctcca taccgatcca tttgatcacg gaaaatattt gtaaataaga
    49801 acagttctgg tttcatatat tctgtgactt tgctcaaact tgcttcatct atttctagga
    49861 cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa gaaagtcgat acaattccct
    49921 gtaccatatt cgcaccagta gggttggtta gaacttcatc gaattgctgg cgcaaaatat
    49981 tgactgttaa agcggtagtc aatgttttac cgtttgttcc ggtgacaacg acgacttggt
    50041 agtcttttgc caaactatct agtatatgtg gatcgacttt cagcgctagt ttacctggta
    50101 aacttgatcc gcctttaaaa aaagttttta atgcccattg cgacgttttt ccaactgcaa
    50161 tcgcaaaatg actgcgtatt cccattatgt aacctccaaa aacgatttgc tgacgaataa
    50221 attcaactaa tcataccata tgttgaaaaa aaggttaaat tgcttttctt attcttaaga
    50281 aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg catgatttaa caaaaaaaca
    50341 gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta ttattccctc ttacctatat
    50401 tcccatttta accgataata attttctcgc ttggatattc atagcctaga tctcgtgttt
    50461 cttttggcac gaataacatc aatgtatata gcattccgat ccgcccaata aacatcagtg
    50521 tagcaatcgt gattttgccc acaacagaca agtctccggt aatccctaaa gataaaccag
    50581 ttgttccaaa cgcagaagtt acttcgatga tgatcgagat cagcgtttgt tcttctgtag
    50641 cagataaaaa aacgatacaa aagaaacaca taccaagaga aagcataaag acaacgactg
    50701 attttcgcac atcatcttga tcgattctcc gtccaaaaat attgatgtta tcttcacttt
    50761 tgaggaagga atatagatac aaaccgatga ttgctacagt agttgttcga atcccgccgc
    50821 ctacagaact aggactgcat ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga
    50881 tttgaaaatc acccaaatcg tgaatctgca gaccagcatt acgagtcgta atcgaataaa
    50941 acatcgaatt gatccatttg acactcatat tggaatcttg gaataaatgg tccttttcca
    51001 atagataaat caagacagtt cccgaaacaa acaagataac aaaagctaac acagctaatt
    51061 tggtaaacaa ggaaaaacga aacggaagct tcgcattgga acgtttgtat aatagccatt
    51121 cccggcactc catcaataca ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca
    51181 ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc
    51241 cagagttcgt gacagccgat atcgcctgat aaaaaccgta aaaaacggca tcacgccaac
    51301 gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa
    51361 accagattaa aatagcaaaa gtgatccgaa tcagtcggac gataccagac aaacgcggtt
    51421 gattcatatc cgtcatgatc agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa
    51481 tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc aacttgaaat aatatttcca
    51541 gcagtataat accgttatca ttgaacacag aattgatatc aaaagtcgat agtccagtca
    51601 cactcactgt actgattgcc atgaacagca tgtccacaaa agaaacatgg ctatctggtt
    51661 cgcgaaaaat cggcatataa aatagaacga gtgataagac cgtcatcaat atataataaa
    51721 aaacgatgat ttggatcgat gagaaatgat tggcagcaaa cttctgtcct cgccgctgca
    51781 gccttcgaaa gaataaatca acatgcacag tactatcctc ctgacaatag tcgtttctac
    51841 cagtatacaa ggggaattag gaatactcaa agaaattatc aaacagataa caatacagct
    51901 ttcgattaaa atagaaatgg cttataataa ggaaaatcct agaaaaaaga aggaggagga
    51961 ataacaaatt gattaatgct caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt
    52021 atgatacagt tctgaaaaaa atgatcatgc aatgggagaa agaagaagta aagcctaaga
    52081 tattaataca tacatgctgt gctccttgca gtacgcacac attggaattc atgtctcaat
    52141 atgcagagat cacgttattt ttttcaaatt cgaatattca tcctaaaagt gagtatttgc
    52201 gaagattaca tgaacaaaaa cgatttgtag accaatttaa tgagaaaact gaccatcatg
    52261 ttgcgctaat cgttgatgaa taccgtccaa atgaattcac caaaatggtt ttaacaaacc
    52321 atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc atgcttcaat atgcgattag
    52381 atcttgttgc gcaaaaagcg caagaactag gatacgatta ctttggaagt gccctgacca
    52441 tctcgccaaa gaaaaatgca gctttgatca atcagatcgg aatggatatc caaaagattt
    52501 acgggacaca atacttgcca agtgatttca agaaaaacaa aggctatgaa cgctctttgc
    52561 aaatgtgcaa agagtatgat atttatcgcc aatgttattg tggttgcgtg ttcgctgcca
    52621 agcagcaagg atgcgatctg aaagaaatca atcgggaagc aaaagcttac ttaaaaacat
    52681 caaccgtagt ttttgaggaa aattaaagaa atctttcgga aaaacgtaag aaaaaaataa
    52741 agaatttatg gtagaaataa ggtagactgt ataatttgtc cgtagtaaag taaggaaaca
    52801 agaataaatt tcaatgaagg aagagataag atgcgaaaag tagcacctat tttgaagcca
    52861 atcgctgtat taacaatctc gacgattgtc gcgactacta caggtattgt gaatagttcc
    52921 agctaccgtg caccaagttc aagttctagt actgtagaag aaacaacacc aactacttca
    52981 gtctcgacat ctgaaccaac aacaacggaa agtttgaatg aaaccactac gacaagtaca
    53041 tgggacgata caacaactag ttcgtcgtat gatgagacat atacaactga cagctcaagc
    53101 gaaacaagta atacggtacc agatggcact ccatcggatt caaccaatcc aagtacaagt
    53161 ccggatacaa gtgctagctc cgaaacgata ccaagcagta cgactgtaga tgataacacg
    53221 caaaatgatg atgcagcacg tacagcacag caaaaccaag attcaggaaa tacccaaacg
    53281 tataaccagc aagcaggtga ttctcaatga catttttact agaccttatc aacggggtgc
    53341 ataaattcct tggctattta gacatcagtc ctaaatatct taaccgtgga tatacgattt
    53401 taagtgtgat tcccacgctt tatttattga gaattgttta tggactttgg caaaaccaaa
    53461 actatcttca atttttctta tatggacttg catttctagt gcttgtttat tttactgttt
    53521 taaatgtatt ttattacttt ttagataaaa acacgaaagc cgatgtcact cagctttttg
    53581 tgaagtattt acctgatgaa gcgttcaaca tccagacaga agaatcgata gataatggaa
    53641 attcgatcga taaagtgaat acacaagagg tcatggtttc ttatgacgaa gattatcaat
    53701 tgaaattagc cgagaatatg cggtatttga ttggaaatgg tgagatcaaa acaaatgaac
    53761 tcggtcgcat ggatggattt ctggtcgata gaaatacatt gtatccttat tactatgtca
    53821 agaaaacagg agagaaagag tatgttctaa atattggacg aagctactcg gacatggaaa
    53881 aagtagggac cattcgactt gactcaacag aagacacatt ggaacctgtc ggattgttca
    53941 tcgtcggcgg tgattttgtc aaagacgggt tacgctatca tgagccttat cgtctgaaat
    54001 tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa
    54061 gaagacattc ataatgtgat gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt
    54121 cggaataagg gggatgtgac acgtcttttg tcacgttcct tttttatata acgagaaaga
    54181 taagacgaac acatcactta ttctttcctt tcatttataa acattgtaga ataaagaaag
    54241 tacaacgaag tattgtgaaa aaacgtaata aataacgagg aaaatacaag taggaggtaa
    54301 gctgtgaaag aatttttgaa aaattggggg attttgatcc ttgtattagc ggctatttta
    54361 ttagctagag tgtacgtgtt tacaccagtt actgtaaacg ggcattcgat ggaccctaca
    54421 ttaagtgatg gacaacgatt gatttcatct aagatctcaa actatgaacg aatggatatt
    54481 attactacaa aggaaccggg ggatgaagaa cggatgattg ttaaacggat catcggtatg
    54541 cctggagata cagtcaagat ggaaaatgac cagttgacca ttaatggcaa aaagtatgat
    54601 gaaccttatc tggatgagtt caaaaaagaa ttttcagaag ataagttaca aggagaatat
    54661 gcttatagct caggattcca agcacaagca gaaagttcat ccacctttac gagtgacttt
    54721 gaatatactg tgccaaaagg aaagtatctt gttctaggcg ataaccgctt gatttcaaaa
    54781 gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt
    54841 tattggcctc tttcagaaat taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat
    54901 gttttttgtc acagcttctt tttccgaaaa ttccttctta tttcttggag ttgctcactt
    54961 tttttgcaat ctcattttaa gctttttggc tgatggataa gaaaagcgaa gcgtttttgt
    55021 tcataaatag cttttcccca cgatggaagg tatgactagt agcagagaca aagcccgttt
    55081 tttccaatcg attattcaat tcttcttggc taaagccatt atggactttc ggatgactaa
    55141 tttgttcatt cttgtcaaag tctacgatga tcaattgacc gccaggagct aaaatttcat
    55201 aaagttttgt aagaatattc tccgtatcag gaatgtgtaa aagtacaagt gatagaagga
    55261 tgagattagc ttggacagca ggaaattcga cagaagcatc agcgtggatc gttcggacat
    55321 ttttcagatc tgctgcttgg atcttttctt ctgccatttt cagcatagtt tctgaagcat
    55381 cggcaatgat cagttctttg aatctttccg caagcggtaa actaacaagc ccagttccgc
    55441 ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg tagataagta gttagttctt
    55501 cgcgaataat cttggctaat gcttgacggt caggggaatc ataatggtta gcgatggaat
    55561 caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt
    55621 atagcacgtg aaaagaaaga aagcatgcta tactcaattc aaaaagggag aatctagaaa
    55681 gaatatgccg atatcgtttg taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat
    55741 attactagac ttgcctgagt ggtttgggct tcctgaatca acggaaaaat atatcgagga
    55801 atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac
    55861 tctttcacag acaagtgaag atactgccga aatctattct attgtatggg aataaaaaaa
    55921 gactatcatc accaaggatt aggaacaaaa ctttttaaag aagcagaagg ttacgcagca
    55981 aaacattaca aatatttgca agtgaagact gtagacgagg ggcactactc tatatatgat
    56041 caaactattt gtttctacga atcattagga ttttcaaggt tggaagtttt tccaaattta
    56101 tgggatgaat ggaacccatg tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata
    56161 gatttaatac taatctgtgc ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag
    56221 tataatacca taggaacaaa ctggaaggtg aaaaagagca tggcacaact attttttaag
    56281 tatggcgcaa tgaatagcgg caagacgatc gagatcctaa aagtcgcaca taattatgaa
    56341 gaacaaaata agccagttgt cttaatgaca agcggtattg atacaagaga tggtttaggc
    56401 gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc cgatatttga agaaacagac
    56461 gtatttgacg taattcagaa aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg
    56521 caattcttaa aaaagaaaca tgtcattgct tttacgagaa ttgtggacga attggatatt
    56581 ccggttatgg catttggttt gaaaaatgat tttcgaaacg agttatttga aggatcaaaa
    56641 tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa caatctgttg gttctgccac
    56701 aaaaaagcaa tcatgaattt acattatatt gatggaaacc ctgtttatga aggcgatcaa
    56761 gtgcaaattg gcggaaatga agcttattac cccgtttgtc gaaaacatta ctttcaccca
    56821 tcaatgatta cagaaggaga ataagcatgt acgatcaatt acaagcaatt gaagaccgtt
    56881 atgaagagct tggagaactt ttaagtgacc cagaagtgat ttctgataca aagcgtttta
    56941 tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata
    57001 aagaagtagt acaaggaatc accgatacag aagaactgct tggtgaaaaa ctggatgatg
    57061 aaatggctga actagcaaaa gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag
    57121 aagaaaaaat caagatcttg cttttaccaa aagatccaaa tgacgataaa aatatcatca
    57181 tggaaatccg tggagcagca ggcggagacg aagcagcatt attcgctggc gatcttttca
    57241 atatgtacca aaagtatgct gaagcacaag gctggaaaac agaagtgatg gaagctaacg
    57301 ttaccggaat cggtggatat aaagaagtga tcatgatgat caccggagac aacgtatatt
    57361 cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc
    57421 aagggcgtat ccatacttcc accgcaacag ttgtcgtgat gccagaagct gaagaagtag
    57481 agatcgatat tgaagacaaa gatatccgta cagatatcta tcatgcttca ggtgctggcg
    57541 gacagcacgt caataaaaca gcatctgctg tacgtttgac tcacttacca acagggatcg
    57601 ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct
    57661 tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca aagcgagtat gatgccaatc
    57721 gaaaatcagc agtcggaact ggggatcgtt cagaacggat ccggacgtat aatttcccgc
    57781 aaaatcgtgt gactgatcat cgtatcggat taactatcca aaaattagat cagattttag
    57841 cagggaaatt agatgagatc gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg
    57901 aagctatgca aaatgggtaa aatgacctat cgagaagtcc tgtcacgggc ttcttctttt
    57961 ttagaggatc atgggaaaga agcatatagt atccaatttt tatttttgga acgaaaacaa
    58021 tggaaaaaac ttgattggct ccttcatatg aacgaagaga tttcagaaga agaacagcgg
    58081 ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc cacctcagta tttgctagga
    58141 tatgcagatt tttatgatca tcggttaaag gtaactgaag caacactgat ccctcgacca
    58201 gaaacagagg agttagtcga atggtgttta gacgagacgc ctggtgtacc gttagaagtg
    58261 atagatattg ggacagggac tggagcaatt gctattagtt tgaaagcagc aagaaaaaat
    58321 tggcatatat ctgctgtgga tctttcagaa gaagcattag aagtagcgaa agaaaatgct
    58381 cagaaagaag gaacgaaaat cagtttttat catggagata cattaggacc agtaatggat
    58441 cagcaatttg atgtgattat ttctaatccg ccgtacatca gtcggaatga atgggaactg
    58501 atggatgaaa gtgtccggtc attcgaacct aaaatggccc tttttgctga gaacgatgga
    58561 ttagctattt atgaaaaaat agcagttgaa gcaccaagcg tactaaaatc aaatggaaag
    58621 atttttttag aaatcggatt tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca
    58681 tttccagata aaaaagtggc gataaaaaag gatctttttg gcaatgaacg aatgatccaa
    58741 gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt ttttttatta taaaaaaaca
    58801 cttatttgac aaggatgtaa acgacttttt ggtttatcaa caagtttatc cactattgac
    58861 aaatagatta tccacaaaca gtggaaaact tgaggataaa ctcagacaaa ataagaatgt
    58921 atgaaaaagg ggttgtggat aaactgtgga aacgaaacga tatgatgaaa cagaattgca
    58981 agaggcagcc gaagcactga aagaaggaga acttgttgct tttcctaccg aaacagtata
    59041 cggattgggc gccaacgctc ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg
    59101 gagaccgcaa gataatccat taatcgtcca tgtcgcctct tttgaacaag taaaagagta
    59161 tgtggacaac tttcatccgg cagcagagaa gattgtgaag aacttctggc ctggaccatt
    59221 aactttgata tttaagatca aaaaagacac tttgccctca gttgtgacgg gaggattatc
    59281 cacagctgca tttcgtatac cggataacaa aaaaacacta gaagtaatcg aactttccgg
    59341 tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga
    59401 tcatgtatat catgatctac aaggaaaaat taccgggatt attgatgatg gagctacgcg
    59461 aattggtgta gaatccactg ttcttgattt aagtgatcca acagccatgc ctatgatttt
    59521 acgaccaggt gcagtaacga aagaacaaat cgaagcagtc atcgaatctc ctgttgctat
    59581 tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc cctgggatga aatacaaaca
    59641 ctactcaccc gatacacgtg ttctgatggt cagagatggc gattggtcaa ctgcagttca
    59701 atgggcaaaa aataagaaga tccgtgcagg tgtcatagca agccccgaaa ttgcggatca
    59761 agtgcggaca gatactgcag ctgtttatat gtataacgac aactcagtag aagcggcggc
    59821 taaaggattg tttgcaggtc taagaggatt agatgaacca acacttggat tagatttgat
    59881 ttttgtacaa gtgtatccag aaactggatt ggggaatgct tacatgaatc gcttgaaaaa
    59941 agcagcagga caaaattatt ttgaaaaatg atcaattggt tattgtcgca ttcttttagt
    60001 atgatacaat gctatcaagt aaatcttcta tcagtgaacg aaaaactaag cgtaactaat
    60061 cgtaatagaa gagatacgaa tatttggcaa tttgtttttg atgggaaaaa taaaattgta
    60121 ggaaaataaa aaggagctgt tgtttgtggt agattacaaa acgtttgacc ctgatctttg
    60181 ggctgcgatc gccaaagaag aagaaagaca agaacataat ttggagctga ttgcctctga
    60241 aaactttgtc tccgaagcag tgatggctgc acaaggaagt atcttgacaa ataaatacgc
    60301 agaaggttat ccagggcatc gctattatgg tgggtgtgaa tttgtcgata tcgttgaaaa
    60361 tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa tttgcgaacg tccagcctca
    60421 ctccggctct caagccaaca cggctgctta tctcgctttg gtagaaccag gggacacgat
    60481 cttaggaatg gatctttccg caggcggaca tctaacacat ggatcaccag tcaacttcag
    60541 tggcaaaacc tatcactttg ttgcttacgg tgttgatccg acaacagaag ttattgatta
    60601 taatgtggta cgtattttag ctagaaaaca tcaaccaaaa ttgatcgtag caggtgccag
    60661 tgcttacgga cgaacgatcg actttgcgaa attcagagaa atcgcagatg aagtaggcgc
    60721 aaaactgatg gtagatatgg cacatatcgc aggattagta gcagcaggac ttcatcctaa
    60781 tccagttcct tatgcagata tcacaacgac taccactcat aaaacattgc gtggccctcg
    60841 aggcggcatg attttgacaa acgatgaagc attagcaaaa aaaataaata gtgcggtatt
    60901 tcctggtatt caaggcggcc ctctagagca tgtgattgcg ggtaaagcag tagcattcaa
    60961 agaagcatta gatcctgcat ttaaagaata tagtgaacaa atcatcgcca atgcaaaagc
    61021 aatggtcaaa gttttcaatc aagcaattgg aaccagagtc atttcagggg cgacagataa
    61081 tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat ggaaaagaag cagagagcat
    61141 tttggacagt gtgaatatca ctgtaaataa aaactcgatt ccttttgaaa cactaagtcc
    61201 attcaaaaca agcggtattc gaatcggcac gccagcaatc actactcgcg ggttcaaaga
    61261 agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca ttacaagcaa aagatgataa
    61321 cgctcaacta gacgaagtca aaacaggtgt acgcgaatta actgaaaaat ttccgttgca
    61381 taaaaaataa aaatttaaat tccattagta aacagaaaag ttcaatgttg ggcttttctg
    61441 tttatttttt gggaaaacgg attctttgac aatatcggtg atgctctcta gtatatgagg
    61501 tgtaatttta gtcttcacac taaatctcag ggaggaaaag tcatgactga tcgtatctcg
    61561 ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc ctcatccgcg tatctatgaa
    61621 ctagcaccgg aagaaggaag agttttacta gaagaagtac aagaatcacc tgtgaacaaa
    61681 tattccgtcg atattgaaga cttgatggtg gatactggcg aatggggaaa actcaatatt
    61741 cgtctcgtac gaccagaagg gaatatggat aaattaccag ttatttttta tattcatgga
    61801 gcaggctggg tttttggcag tgctcaaacc catgataaat tgatcagaga acttgcggtt
    61861 cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat caccagaagc aaaatatcca
    61921 acagcaattg aacaaaatta tgctgtgttg caacagctga ataaattagc caagtcaaaa
    61981 aatctggatc tcgagaatct aatcgttgca ggagattcag taggtggtaa catggctaca
    62041 gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa tatccaagca attgctgtat
    62101 tatccagtca caaacgctga ttttgataca gattcctatc atgaattcgg agaaaactat
    62161 tttttgacaa gagaaggaat gatgtggttt tgggaccaat atacgacgaa tgaagaagag
    62221 cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa atgaactaaa tgatcttcct
    62281 tcagcaatga ttttaacggg agaagcggat gtattaagag atgaaggtga ggcttatgcg
    62341 cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac gcttccaagg aatcatccat
    62401 gattttgtca tggtaaatgc tatggatcag acaaaggcaa cgcgtgcagc aatggcatta
    62461 tctacttctt ggatcaagaa aaaagaataa gctttgcttg tccgcaggaa aacttctttt
    62521 caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact tgattttgtt
    62581 ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca aatttcaagt
    62641 gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa actgcggaac
    62701 aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg aagtatcacg
    62761 ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac
    62821 gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg gaatggttga
    62881 cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt atcgagatga
    62941 agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg atgcacgtca
    63001 gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg caatcgatgc
    63061 attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag cacctgaggg
    63121 agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt ctctagatga
    63181 aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg accgtttgtt
    63241 tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt
    63301 ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg catataaaaa
    63361 aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa
    63421 cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct
    63481 caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa
    63541 ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat ggtacgatag
    63601 ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac atcaattata
    63661 ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact ctttacaatc
    63721 agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt tggtagagca
    63781 acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta aaagagaaga
    63841 cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta aaggaaataa
    63901 agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg agaataaaac
    63961 gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg taatgatcga
    64021 gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta tcaaaaggat
    64081 gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga taaatccaaa
    64141 gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa aaatagcgag
    64201 aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa taaataataa
    64261 cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa
    64321 aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg ctatattttt
    64381 gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa aaacaagtgt
    64441 aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg gcgcaaatag
    64501 caaccctaac aaaccgagta caacgacaat agtgcccaat atcccttttt taagttctat
    64561 tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt tgaagataga
    64621 gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt gtattgctaa
    64681 aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa ttttctagca
    64741 aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga gcgtacttca
    64801 atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc aaaaaagcta
    64861 gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc tacaaaatct
    64921 gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc agataaattt
    64981 acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc catcaattta
    65041 gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt tgtcaatata
    65101 cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct ttgaaaatca
    65161 ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg gcagattatt
    65221 tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc ttcgttttca
    65281 tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg aaaaatcata
    65341 tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac ttacattagc
    65401 agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc tcttgaaacg
    65461 ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa tcttagaata
    65521 aaaattttta ctgatttaat ttagaatgtt tttaaaataa aaatcgttgc cataacagtg
    65581 tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt
    65641 tgtttataga atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag
    65701 tgaagaaata gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa
    65761 agaaaaggaa cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta
    65821 tcctaaagtc ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt
    65881 gtctaaacca gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga
    65941 attaggtcga ggaatggctt cggcaccgat tacttttggg catttataaa aacgagttat
    66001 tcatctcaat gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt
    66061 gaacagagtc tgtatttttt tattctaaat aaagaaacag atgtttcttt atattgtatt
    66121 ttatccttta attaacacaa aaacagctag tataaactaa taatgttaat taaaagtgta
    66181 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
    66241 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
    66301 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
    66361 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
    66421 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
    66481 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
    66541 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
    66601 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
    66661 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
    66721 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
    66781 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
    66841 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
    66901 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
    66961 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
    67021 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
    67081 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
    67141 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
    67201 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
    67261 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
    67321 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
    67381 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
    67441 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
    67501 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
    67561 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
    67621 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
    67681 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
    67741 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
    67801 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
    67861 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
    67921 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
    67981 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
    68041 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
    68101 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
    68161 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
    68221 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
    68281 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
    68341 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
    68401 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
    68461 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
    68521 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
    68581 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
    68641 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
    68701 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
    68761 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
    68821 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
    68881 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
    68941 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
    69001 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
    69061 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
    69121 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
    69181 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
    69241 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
    69301 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
    69361 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
    69421 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
    69481 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
    69541 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
    69601 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
    69661 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
    69721 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
    69781 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
    69841 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
    69901 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
    69961 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
    70021 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
    70081 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
    70141 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
    70201 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
    70261 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
    70321 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
    70381 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
    70441 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
    70501 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
    70561 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
    70621 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
    70681 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
    70741 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
    70801 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
    70861 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
    70921 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
    70981 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
    71041 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
    71101 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
    71161 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
    71221 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
    71281 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
    71341 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
    71401 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
    71461 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
    71521 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
    71581 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
    71641 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
    71701 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
    71761 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
    71821 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
    71881 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
    71941 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
    72001 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
    72061 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
    72121 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
    72181 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
    72241 acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
    72301 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
    72361 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
    72421 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
    72481 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
    72541 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
    72601 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
    72661 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
    72721 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
    72781 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
    72841 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
    72901 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
    72961 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
    73021 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
    73081 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
    73141 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
    73201 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
    73261 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
    73321 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
    73381 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
    73441 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
    73501 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
    73561 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
    73621 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
    73681 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
    73741 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
    73801 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
    73861 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
    73921 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
    73981 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
    74041 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
    74101 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
    74161 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
    74221 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
    74281 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
    74341 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
    74401 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
    74461 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
    74521 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
    74581 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
    74641 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
    74701 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
    74761 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
    74821 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
    74881 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
    74941 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
    75001 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
    75061 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
    75121 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
    75181 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
    75241 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
    75301 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
    75361 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
    75421 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
    75481 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
    75541 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
    75601 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
    75661 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
    75721 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
    75781 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
    75841 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
    75901 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
    75961 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
    76021 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
    76081 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
    76141 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    76201 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    76261 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    76321 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    76381 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    76441 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    76501 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    76561 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    76621 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    76681 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    76741 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    76801 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    76861 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    76921 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    76981 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    77041 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    77101 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    77161 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    77221 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    77281 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    77341 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    77401 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    77461 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    77521 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    77581 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    77641 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    77701 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    77761 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    77821 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    77881 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    77941 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    78001 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    78061 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    78121 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    78181 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    78241 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    78301 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    78361 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    78421 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    78481 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    78541 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    78601 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    78661 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    78721 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    78781 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    78841 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    78901 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    78961 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    79021 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    79081 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    79141 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    79201 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    79261 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    79321 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    79381 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    79441 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    79501 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    79561 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    79621 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    79681 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    79741 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    79801 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    79861 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    79921 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    79981 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    80041 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    80101 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    80161 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    80221 tgtac
//
LOCUS       DACECB010000005        76090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECB010000005 DACECB010000000
VERSION     DACECB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.005"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO04_000364"
     CDS             <1..411
                     /locus_tag="IWO04_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0212000.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IWO04_000365"
     CDS             509..697
                     /locus_tag="IWO04_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0212001.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IWO04_000366"
     CDS             complement(755..1108)
                     /locus_tag="IWO04_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0212002.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IWO04_000367"
     CDS             1639..2061
                     /locus_tag="IWO04_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0212003.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IWO04_000368"
     CDS             2058..2288
                     /locus_tag="IWO04_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212004.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IWO04_000369"
     CDS             2786..2986
                     /locus_tag="IWO04_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0212005.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IWO04_000370"
     CDS             3013..4206
                     /locus_tag="IWO04_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0212006.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO04_000371"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO04_000371"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0212007.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IWO04_000372"
     CDS             complement(5898..6821)
                     /locus_tag="IWO04_000372"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0212008.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IWO04_000373"
     CDS             7017..7622
                     /locus_tag="IWO04_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0212009.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IWO04_000374"
     CDS             7930..8946
                     /locus_tag="IWO04_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0212010.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IWO04_000375"
     CDS             9087..10388
                     /locus_tag="IWO04_000375"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0212011.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IWO04_000376"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IWO04_000376"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0212012.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IWO04_000377"
     CDS             11117..11515
                     /locus_tag="IWO04_000377"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0212013.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IWO04_000378"
     CDS             11581..12663
                     /locus_tag="IWO04_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0212014.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IWO04_000379"
     CDS             12875..14437
                     /locus_tag="IWO04_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212015.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IWO04_000380"
     CDS             14430..15563
                     /locus_tag="IWO04_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212016.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IWO04_000381"
     CDS             15563..16519
                     /locus_tag="IWO04_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212017.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IWO04_000382"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IWO04_000382"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0212018.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IWO04_000383"
     CDS             17808..18626
                     /locus_tag="IWO04_000383"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0212019.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IWO04_000384"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IWO04_000384"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0212020.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO04_000385"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO04_000385"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0212021.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IWO04_000386"
     CDS             complement(20506..21087)
                     /locus_tag="IWO04_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0212022.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO04_000387"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO04_000387"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0212023.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO04_000388"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO04_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0212024.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO04_000389"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO04_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0212025.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IWO04_000390"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IWO04_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0212026.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IWO04_000391"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IWO04_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0212027.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO04_000392"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO04_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0212028.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IWO04_000393"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IWO04_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0212029.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IWO04_000394"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IWO04_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0212030.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IWO04_000395"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IWO04_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0212031.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IWO04_000396"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IWO04_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0212032.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO04_000397"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO04_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0212033.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="IWO04_000398"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="IWO04_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0212034.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO04_000399"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO04_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0212035.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="IWO04_000400"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="IWO04_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0212036.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO04_000401"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO04_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0212037.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO04_000402"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO04_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0212038.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="IWO04_000403"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="IWO04_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0212039.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="IWO04_000404"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="IWO04_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0212040.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="IWO04_000405"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="IWO04_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0212041.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="IWO04_000406"
     CDS             33541..33726
                     /locus_tag="IWO04_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0212042.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO04_000407"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO04_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0212043.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="IWO04_000408"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="IWO04_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0212044.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="IWO04_000409"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="IWO04_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0212045.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO04_000410"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO04_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0212046.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO04_000411"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO04_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0212047.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="IWO04_000412"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="IWO04_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0212048.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="IWO04_000413"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="IWO04_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0212049.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="IWO04_000414"
     CDS             37801..38448
                     /locus_tag="IWO04_000414"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0212050.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="IWO04_000415"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="IWO04_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0212051.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO04_000416"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO04_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0212052.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO04_000417"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO04_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0212053.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO04_000418"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO04_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0212054.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="IWO04_000419"
     CDS             39886..40824
                     /locus_tag="IWO04_000419"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0212055.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO04_000420"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO04_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0212056.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="IWO04_000421"
     CDS             41504..42343
                     /locus_tag="IWO04_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0212057.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="IWO04_000422"
     CDS             42319..43188
                     /locus_tag="IWO04_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0212058.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="IWO04_000423"
     CDS             43178..43975
                     /locus_tag="IWO04_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0212059.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="IWO04_000424"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="IWO04_000424"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0212060.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="IWO04_000425"
     CDS             complement(45257..47926)
                     /locus_tag="IWO04_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0212061.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="IWO04_000426"
     CDS             complement(48164..48352)
                     /locus_tag="IWO04_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0212062.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="IWO04_000427"
     CDS             48622..48984
                     /locus_tag="IWO04_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0212063.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="IWO04_000428"
     CDS             49036..50718
                     /locus_tag="IWO04_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0212064.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="IWO04_000429"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="IWO04_000429"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0212065.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="IWO04_000430"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="IWO04_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0212066.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="IWO04_000431"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="IWO04_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0212067.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="IWO04_000432"
     CDS             54553..55557
                     /locus_tag="IWO04_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212068.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="IWO04_000433"
     CDS             55558..56502
                     /locus_tag="IWO04_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212069.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="IWO04_000434"
     CDS             56502..57464
                     /locus_tag="IWO04_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212070.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="IWO04_000435"
     CDS             57491..58405
                     /locus_tag="IWO04_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212071.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="IWO04_000436"
     CDS             58431..60212
                     /locus_tag="IWO04_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0212072.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="IWO04_000437"
     CDS             60560..61246
                     /locus_tag="IWO04_000437"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0212073.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="IWO04_000438"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="IWO04_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0212074.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="IWO04_000439"
     CDS             64856..65665
                     /locus_tag="IWO04_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0212075.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO04_000440"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO04_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0212076.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO04_000441"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO04_000441"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0212077.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="IWO04_000442"
     CDS             complement(69217..69669)
                     /locus_tag="IWO04_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0212078.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="IWO04_000443"
     CDS             69819..70769
                     /locus_tag="IWO04_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0212079.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="IWO04_000444"
     CDS             70762..71721
                     /locus_tag="IWO04_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0212080.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="IWO04_000445"
     CDS             71718..72473
                     /locus_tag="IWO04_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212081.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="IWO04_000446"
     CDS             72496..73449
                     /locus_tag="IWO04_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0212082.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(73972..74268)
                     /locus_tag="IWO04_000447"
     CDS             complement(73972..74268)
                     /locus_tag="IWO04_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0212083.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74623..74979
                     /locus_tag="IWO04_000448"
     CDS             74623..74979
                     /locus_tag="IWO04_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0212084.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            74989..75888
                     /locus_tag="IWO04_000449"
     CDS             74989..75888
                     /locus_tag="IWO04_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0212085.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24796 a        13752 c        16323 g        21219 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    73921 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    73981 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    74041 cttactccac cgttttaata attgatagaa ataactctct tctgctttga ttttatactc
    74101 gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa
    74161 tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat
    74221 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
    74281 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
    74341 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
    74401 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
    74461 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
    74521 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
    74581 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
    74641 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
    74701 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
    74761 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
    74821 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
    74881 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
    74941 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
    75001 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
    75061 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
    75121 aacaaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
    75181 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
    75241 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
    75301 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
    75361 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
    75421 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
    75481 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
    75541 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
    75601 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
    75661 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
    75721 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
    75781 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
    75841 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
    75901 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
    75961 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
    76021 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
    76081 aaaaaaaata
//
LOCUS       DACECB010000006        74407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECB010000006 DACECB010000000
VERSION     DACECB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.006"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IWO04_000450"
     CDS             complement(179..586)
                     /locus_tag="IWO04_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0212086.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IWO04_000451"
     CDS             complement(775..1602)
                     /locus_tag="IWO04_000451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ0212087.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IWO04_000452"
     CDS             1911..2129
                     /locus_tag="IWO04_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212088.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IWO04_000453"
     CDS             2150..2785
                     /locus_tag="IWO04_000453"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0212089.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IWO04_000454"
     CDS             complement(2978..3217)
                     /locus_tag="IWO04_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212090.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IWO04_000455"
     CDS             complement(3241..4764)
                     /locus_tag="IWO04_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0212091.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IWO04_000456"
     CDS             complement(4782..4904)
                     /locus_tag="IWO04_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0212092.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IWO04_000457"
     CDS             complement(4934..5917)
                     /locus_tag="IWO04_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0212093.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWO04_000458"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWO04_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0212094.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IWO04_000459"
     CDS             complement(6111..6488)
                     /locus_tag="IWO04_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212095.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWO04_000460"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWO04_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0212096.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWO04_000461"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWO04_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0212097.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IWO04_000462"
     CDS             complement(7506..9278)
                     /locus_tag="IWO04_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212098.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IWO04_000463"
     CDS             complement(9232..10986)
                     /locus_tag="IWO04_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212099.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IWO04_000464"
     CDS             complement(11096..11665)
                     /locus_tag="IWO04_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0212100.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11683..13107)
                     /locus_tag="IWO04_000465"
     CDS             complement(11683..13107)
                     /locus_tag="IWO04_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0212101.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13109..13777)
                     /locus_tag="IWO04_000466"
     CDS             complement(13109..13777)
                     /locus_tag="IWO04_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0212102.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13908..14492)
                     /locus_tag="IWO04_000467"
     CDS             complement(13908..14492)
                     /locus_tag="IWO04_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0212103.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14823..15056)
                     /locus_tag="IWO04_000468"
     CDS             complement(14823..15056)
                     /locus_tag="IWO04_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0212104.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15071..16021)
                     /locus_tag="IWO04_000469"
     CDS             complement(15071..16021)
                     /locus_tag="IWO04_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212105.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16018..16860)
                     /locus_tag="IWO04_000470"
     CDS             complement(16018..16860)
                     /locus_tag="IWO04_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0212106.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16993..17718)
                     /locus_tag="IWO04_000471"
     CDS             complement(16993..17718)
                     /locus_tag="IWO04_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212107.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18179..18493)
                     /locus_tag="IWO04_000472"
     CDS             complement(18179..18493)
                     /locus_tag="IWO04_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0212108.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18509..20104)
                     /locus_tag="IWO04_000473"
     CDS             complement(18509..20104)
                     /locus_tag="IWO04_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ0212109.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWO04_000474"
     CDS             complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWO04_000474"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ0212110.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWO04_000475"
     CDS             complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWO04_000475"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ0212111.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVCLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22083..23090)
                     /locus_tag="IWO04_000476"
     CDS             complement(22083..23090)
                     /locus_tag="IWO04_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0212112.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23203..24363)
                     /locus_tag="IWO04_000477"
     CDS             complement(23203..24363)
                     /locus_tag="IWO04_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ0212113.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24439..25893)
                     /locus_tag="IWO04_000478"
     CDS             complement(24439..25893)
                     /locus_tag="IWO04_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ0212114.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(25957..26781)
                     /gene="iolB"
                     /locus_tag="IWO04_000479"
     CDS             complement(25957..26781)
                     /gene="iolB"
                     /locus_tag="IWO04_000479"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ0212115.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(26820..28736)
                     /gene="iolD"
                     /locus_tag="IWO04_000480"
     CDS             complement(26820..28736)
                     /gene="iolD"
                     /locus_tag="IWO04_000480"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ0212116.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(28747..29781)
                     /gene="iolC"
                     /locus_tag="IWO04_000481"
     CDS             complement(28747..29781)
                     /gene="iolC"
                     /locus_tag="IWO04_000481"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ0212117.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(29938..30954)
                     /locus_tag="IWO04_000482"
     CDS             complement(29938..30954)
                     /locus_tag="IWO04_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212118.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(31056..31253)
                     /locus_tag="IWO04_000483"
     CDS             complement(31056..31253)
                     /locus_tag="IWO04_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212119.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(31225..32544)
                     /locus_tag="IWO04_000484"
     CDS             complement(31225..32544)
                     /locus_tag="IWO04_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ0212120.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(33285..34265)
                     /locus_tag="IWO04_000485"
     CDS             complement(33285..34265)
                     /locus_tag="IWO04_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0212121.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(34278..35156)
                     /locus_tag="IWO04_000486"
     CDS             complement(34278..35156)
                     /locus_tag="IWO04_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0212122.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(35172..35972)
                     /locus_tag="IWO04_000487"
     CDS             complement(35172..35972)
                     /locus_tag="IWO04_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ0212123.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            37364..37690
                     /locus_tag="IWO04_000488"
     CDS             37364..37690
                     /locus_tag="IWO04_000488"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0212124.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            37698..38606
                     /locus_tag="IWO04_000489"
     CDS             37698..38606
                     /locus_tag="IWO04_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0212125.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            39235..39486
                     /locus_tag="IWO04_000490"
     CDS             39235..39486
                     /locus_tag="IWO04_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ0212126.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            40028..40186
                     /locus_tag="IWO04_000491"
     CDS             40028..40186
                     /locus_tag="IWO04_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212127.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            40258..41484
                     /locus_tag="IWO04_000492"
     CDS             40258..41484
                     /locus_tag="IWO04_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0212128.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(41573..41965)
                     /gene="rpsI"
                     /locus_tag="IWO04_000493"
     CDS             complement(41573..41965)
                     /gene="rpsI"
                     /locus_tag="IWO04_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0212129.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(41981..42424)
                     /gene="rplM"
                     /locus_tag="IWO04_000494"
     CDS             complement(41981..42424)
                     /gene="rplM"
                     /locus_tag="IWO04_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0212130.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(42943..43410)
                     /locus_tag="IWO04_000495"
     CDS             complement(42943..43410)
                     /locus_tag="IWO04_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0212131.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(43530..45068)
                     /locus_tag="IWO04_000496"
     CDS             complement(43530..45068)
                     /locus_tag="IWO04_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0212132.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            45148..45834
                     /locus_tag="IWO04_000497"
     CDS             45148..45834
                     /locus_tag="IWO04_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0212133.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(46035..46562)
                     /gene="msrA"
                     /locus_tag="IWO04_000498"
     CDS             complement(46035..46562)
                     /gene="msrA"
                     /locus_tag="IWO04_000498"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0212134.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(46646..47323)
                     /gene="pgmB"
                     /locus_tag="IWO04_000499"
     CDS             complement(46646..47323)
                     /gene="pgmB"
                     /locus_tag="IWO04_000499"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0212135.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(47320..48450)
                     /locus_tag="IWO04_000500"
     CDS             complement(47320..48450)
                     /locus_tag="IWO04_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0212136.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(48450..50633)
                     /locus_tag="IWO04_000501"
     CDS             complement(48450..50633)
                     /locus_tag="IWO04_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0212137.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(50630..51655)
                     /locus_tag="IWO04_000502"
     CDS             complement(50630..51655)
                     /locus_tag="IWO04_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0212138.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(51700..52527)
                     /locus_tag="IWO04_000503"
     CDS             complement(51700..52527)
                     /locus_tag="IWO04_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0212139.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(52524..53591)
                     /locus_tag="IWO04_000504"
     CDS             complement(52524..53591)
                     /locus_tag="IWO04_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0212140.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(53617..54453)
                     /locus_tag="IWO04_000505"
     CDS             complement(53617..54453)
                     /locus_tag="IWO04_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0212141.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(54450..55319)
                     /locus_tag="IWO04_000506"
     CDS             complement(54450..55319)
                     /locus_tag="IWO04_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0212142.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(55389..56672)
                     /locus_tag="IWO04_000507"
     CDS             complement(55389..56672)
                     /locus_tag="IWO04_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212143.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(56686..58371)
                     /locus_tag="IWO04_000508"
     CDS             complement(56686..58371)
                     /locus_tag="IWO04_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0212144.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            58646..59671
                     /locus_tag="IWO04_000509"
     CDS             58646..59671
                     /locus_tag="IWO04_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0212145.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(60266..63919)
                     /gene="rpoC"
                     /locus_tag="IWO04_000510"
     CDS             complement(60266..63919)
                     /gene="rpoC"
                     /locus_tag="IWO04_000510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0212146.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(63998..67615)
                     /gene="rpoB"
                     /locus_tag="IWO04_000511"
     CDS             complement(63998..67615)
                     /gene="rpoB"
                     /locus_tag="IWO04_000511"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0212147.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            68343..69311
                     /locus_tag="IWO04_000512"
     CDS             68343..69311
                     /locus_tag="IWO04_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212148.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            69329..70234
                     /locus_tag="IWO04_000513"
     CDS             69329..70234
                     /locus_tag="IWO04_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212149.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            70231..71037
                     /locus_tag="IWO04_000514"
     CDS             70231..71037
                     /locus_tag="IWO04_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212150.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            71194..72162
                     /locus_tag="IWO04_000515"
     CDS             71194..72162
                     /locus_tag="IWO04_000515"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0212151.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(72479..73003)
                     /locus_tag="IWO04_000516"
     CDS             complement(72479..73003)
                     /locus_tag="IWO04_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0212152.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            73290..>74407
                     /gene="scm"
                     /locus_tag="IWO04_000517"
     CDS             73290..>74407
                     /gene="scm"
                     /locus_tag="IWO04_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0212153.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT        21763 a        15725 c        12812 g        24107 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
    11761 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
    11821 ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
    11881 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
    11941 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
    12001 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
    12061 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
    12121 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
    12181 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
    12241 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
    12301 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
    12361 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
    12421 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
    12481 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
    12541 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
    12601 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
    12661 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
    12721 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
    12781 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
    12841 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
    12901 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
    12961 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tacctttttt
    13021 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
    13081 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
    13141 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
    13201 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
    13261 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
    13321 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
    13381 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
    13441 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
    13501 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
    13561 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
    13621 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
    13681 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
    13741 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
    13801 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
    13861 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
    13921 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    13981 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    14041 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    14101 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
    14161 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
    14221 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    14281 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    14341 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    14401 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
    14461 actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
    14521 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
    14581 cttgaagttt caatcgttaa cgtctattac tgaactcatt ccttagagct aaactaatga
    14641 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    14701 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    14761 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagcc
    14821 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    14881 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
    14941 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    15001 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
    15061 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    15121 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    15181 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
    15241 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    15301 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    15361 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    15421 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    15481 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    15541 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
    15601 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    15661 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
    15721 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    15781 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    15841 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
    15901 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
    15961 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    16021 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    16081 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    16141 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
    16201 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
    16261 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
    16321 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
    16381 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
    16441 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
    16501 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
    16561 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    16621 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    16681 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    16741 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    16801 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    16861 attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc
    16921 tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata atatatttat
    16981 tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt
    17041 ttttaaattc tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt
    17101 caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag
    17161 cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac
    17221 taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca
    17281 tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga
    17341 atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt
    17401 aattctttga aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt
    17461 tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt
    17521 tttattgata tcttcattat taatggagac ctctccctga tgagggataa tatttaacat
    17581 tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc
    17641 ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag
    17701 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
    17761 gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt ttttctgttg
    17821 ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt
    17881 tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga
    17941 tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt
    18001 taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt
    18061 gaataaatac aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg
    18121 cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt
    18181 aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt
    18241 tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct
    18301 cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt
    18361 cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg
    18421 tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata
    18481 gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca
    18541 cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag
    18601 gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt
    18661 tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga
    18721 aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg
    18781 tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga
    18841 gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg
    18901 caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt
    18961 taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat
    19021 ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc
    19081 caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa
    19141 aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga
    19201 tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc
    19261 ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg
    19321 tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa
    19381 cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt
    19441 cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca
    19501 gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc
    19561 cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg
    19621 cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg
    19681 atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg
    19741 aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga
    19801 tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag
    19861 cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga
    19921 ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta
    19981 agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag
    20041 aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat
    20101 tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg
    20161 gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg
    20221 atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc
    20281 ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc
    20341 ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga
    20401 tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta
    20461 tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc
    20521 gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg
    20581 gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa
    20641 actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca
    20701 gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct
    20761 tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc
    20821 tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg
    20881 ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta
    20941 ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat
    21001 tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt
    21061 cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc
    21121 attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat
    21181 gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc
    21241 gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt
    21301 tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa
    21361 agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg
    21421 atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc
    21481 ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct
    21541 gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata
    21601 acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg
    21661 atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc
    21721 gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa
    21781 gacatgcttg ccattttcca tcgccaagca gacttggtca cagtgaaagc ctgatggtga
    21841 aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt
    21901 atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc
    21961 agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa
    22021 tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag
    22081 aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt
    22141 tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta
    22201 ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag
    22261 gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat
    22321 ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca
    22381 catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg
    22441 gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt
    22501 tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca
    22561 tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg
    22621 ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt
    22681 gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat
    22741 ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct
    22801 aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca
    22861 tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct
    22921 tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca
    22981 ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata
    23041 tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc
    23101 ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt
    23161 ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac
    23221 gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct
    23281 ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc
    23341 ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag
    23401 ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat
    23461 cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat
    23521 cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg
    23581 aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat
    23641 ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat
    23701 tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata
    23761 agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac
    23821 agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat
    23881 catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc
    23941 agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt
    24001 tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc
    24061 tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa
    24121 aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc
    24181 cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg
    24241 ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat
    24301 aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt
    24361 cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca
    24421 gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg
    24481 atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg
    24541 cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag
    24601 catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag
    24661 acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt
    24721 tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat
    24781 cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg
    24841 accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg
    24901 ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc
    24961 gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc
    25021 ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc
    25081 aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt
    25141 cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc
    25201 accgacattt tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt
    25261 gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt
    25321 ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac
    25381 caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa
    25441 attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat
    25501 atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg
    25561 ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt
    25621 ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt
    25681 gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc
    25741 ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc
    25801 ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg
    25861 accatcaata taatttctta aaaatcttgt catttatttt cctcctaaaa acctaactaa
    25921 acctaactaa actttatgta tctaacagct taataactac ttgcgctgca agatccattc
    25981 gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca catttaaata
    26041 gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa cttcgacgac
    26101 attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt agacccgctg
    26161 gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta aaaaagactc
    26221 atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat taccgccctc
    26281 ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg gtaaaatgtt
    26341 gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat cctccgcgcg
    26401 aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca atcggacatt
    26461 gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac tgtcggttgg
    26521 aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt ctgctgcttt
    26581 aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc cggtttcctt
    26641 gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca aagcagaatt
    26701 agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt ttaatggttg
    26761 atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa aagcaatatt
    26821 taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc ctgcatggct
    26881 gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta gccttctgtc
    26941 atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt cttcgcatcc
    27001 tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc accatagcct
    27061 tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct gaattcggtt
    27121 ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa accagaatta
    27181 tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag ctcgctatgc
    27241 aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg tcgagccatt
    27301 cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta ttccaaatga
    27361 taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa tgaaccggct
    27421 gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa gacctctgtt
    27481 tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg agaaaaatga
    27541 tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa ccgatttcgc
    27601 tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt ttggtaacct
    27661 tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac ttgaaaggct
    27721 tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc ttgttcaaaa
    27781 ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat ccctatgatc
    27841 agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc cattccgccg
    27901 agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc aactagcgga
    27961 atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata tttgcagccg
    28021 cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc ttcagcaagc
    28081 tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa aaattcctcc
    28141 ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc cggacccatc
    28201 gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa ctgctctggt
    28261 cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt cgaaaaggct
    28321 acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt agcaaacgta
    28381 tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc tgctgttaac
    28441 agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt gcgtaaattc
    28501 tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc gctataactt
    28561 ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt tccgtgacca
    28621 tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct gatgtattgt
    28681 tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt ttccattttt
    28741 ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg taataaaatc
    28801 ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga ccatcattga
    28861 tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga ataaaccgta
    28921 taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt ccaccggaaa
    28981 cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt tcattccatg
    29041 tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt cattatctga
    29101 catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac ccatgataat
    29161 gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt tcttccagtt
    29221 ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg ccagcatcac
    29281 tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg agattaatag
    29341 tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct gcaggtctgc
    29401 ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt cggtaaaagt
    29461 cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga tgccttgttc
    29521 attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc cgataaaacc
    29581 agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc cgcccacaaa
    29641 tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt gatcattgta
    29701 ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt cttttgattg
    29761 gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat ttctgaggca
    29821 agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata ttgcgtgctg
    29881 tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac gcctagatta
    29941 aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg taaccgtttt
    30001 attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa caaatccatc
    30061 ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt tcttagtcaa
    30121 atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg ctcgggcaat
    30181 tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat tctccaaggt
    30241 gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa cacccgtcag
    30301 ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc gtaagctgtt
    30361 gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt ttgtcagcat
    30421 acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa caaaacactg
    30481 tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt gatcggtcaa
    30541 ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga tcccttttac
    30601 tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga ccccgtctac
    30661 ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag attccagacc
    30721 tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc ccagcttgag
    30781 cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct tggcgattgc
    30841 tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg cccgatccac
    30901 tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg ccatcttgcc
    30961 tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca ccggagaatg
    31021 aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact gatcttaaac
    31081 tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca tttttcacag
    31141 gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa aaattttact
    31201 acatccattg ggattgattc tttttcacca caacttagac atataaattt cacgttggcg
    31261 ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat actgttgcct
    31321 gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg catggttatc
    31381 tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa cgaattcata
    31441 gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg ttattctttc
    31501 ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt ttactcgttt
    31561 ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat agattccata
    31621 gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag ctatgaattc
    31681 gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt agttgaacat
    31741 gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac gcttcatata
    31801 acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag gagcatttac
    31861 gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa ctctttgctt
    31921 gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc agtattttct
    31981 taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg ttaagccacc
    32041 catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa aggtgtggat
    32101 tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa taatttttgc
    32161 ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca taaagatttc
    32221 ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa acatatcatt
    32281 cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac agaactttcc
    32341 acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa acagtcggta
    32401 tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga cgactagccg
    32461 aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt caaaaaagat
    32521 tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca aaatgaaaag
    32581 ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt tctttactgt
    32641 ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg
    32701 tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg gtaattttta
    32761 ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta atttcatttc
    32821 tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata tttttcaact
    32881 tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact aacttcttta
    32941 acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat ttcatcaaat
    33001 gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa ttttagtata
    33061 acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc cacccaaaag
    33121 ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac aagagatgtt
    33181 ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc tttgttttct
    33241 ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa aatcaatttt
    33301 cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac atctaataca
    33361 gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc gatcaattct
    33421 tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata cccatcttgc
    33481 agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc aaaaataaca
    33541 acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt gtaagcaccg
    33601 actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc acgttttgaa
    33661 gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt ttttgttggg
    33721 cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc acgctcaaaa
    33781 tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc ttctaattgt
    33841 gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc tcttgcaaga
    33901 agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata agtaccatcc
    33961 aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc tggtacactt
    34021 ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc tagcgcgcct
    34081 tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt tggcacatcg
    34141 atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag tgcttttttg
    34201 tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt tttaagaaat
    34261 aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact gcccaacatc
    34321 gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata ggtatctaaa
    34381 gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga agtaaaaagt
    34441 gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat cggtactgag
    34501 aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc tagcaagtag
    34561 acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc accatagaca
    34621 aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc tgttccgttt
    34681 gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac caatcctatg
    34741 ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg tcctaaatat
    34801 gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac gttgatggca
    34861 gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt caacgttccg
    34921 ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc gatagcaaag
    34981 tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg caacagacgc
    35041 atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct tagaaagtaa
    35101 ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg aatcataaaa
    35161 aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata ttttgcttta
    35221 agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt gacatattct
    35281 tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga ttgcagttct
    35341 tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat ccatcttgct
    35401 ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc taagggaaga
    35461 tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct tttttcagct
    35521 tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg aatgagcaaa
    35581 ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc gataccatca
    35641 actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt cttggtaaga
    35701 aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt tgccgagatg
    35761 gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt taatagtttt
    35821 tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac ttcatatcct
    35881 gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc tgaaaaatca
    35941 tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta tttttagtag
    36001 ctactacggt aacaactata aattcttttt gattatctgt caagagttag ttcaataaca
    36061 cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt ataatatgaa
    36121 tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag aaaaacaacc
    36181 tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt tccagtttta
    36241 ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat tctcctcttt
    36301 atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa aagcatttct
    36361 aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt taactatttg
    36421 atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga cctttcggga
    36481 gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt tcgtatacct
    36541 aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa aacaaggggc
    36601 ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc cttgttttgt
    36661 cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac gaaaatgacc
    36721 tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct cccaaaatgt
    36781 cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt aagtattaaa
    36841 taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca aaaataaact
    36901 gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta atttatcgat
    36961 tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca gcgaatccag
    37021 cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc ttcctacgaa
    37081 aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta ttccgtgtta
    37141 ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg gcggggctgt
    37201 atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat caatgaatgg
    37261 cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc gccggatgac
    37321 cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca aaatctttcg
    37381 taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta tcggaatcac
    37441 agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta caaaaggtct
    37501 caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt tggaatcttc
    37561 cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg tcacactggc
    37621 tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag atgcaaaaat
    37681 ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct ggtcttgaaa
    37741 tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg aaaacaagtg
    37801 aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat gatgttttta
    37861 gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa catcccggtg
    37921 aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt gaagaaatcc
    37981 aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc caatgcctca
    38041 accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt caagctcttg
    38101 cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta gacactcagc
    38161 caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg tttccaactt
    38221 cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt gcccgacgaa
    38281 caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt gtacagattg
    38341 ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat cgtagaatca
    38401 aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc ctgaccgcta
    38461 tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg gagccaaaac
    38521 ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta agaaaacgcg
    38581 gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct aaatagactc
    38641 gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac cgttcttcag
    38701 gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact ctcctatagc
    38761 ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc cgtaataagc
    38821 ctcctagtat ataggcagac tgccaattat aactgattca atccacatta tgataaaatc
    38881 caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac gagtcttttc
    38941 attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa agataattag
    39001 tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt tgaatttgtt
    39061 gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat cactgcagct
    39121 gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta ttatcaagca
    39181 gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct gttgattaaa
    39241 tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt actcgaatgt
    39301 gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc taccgaatca
    39361 ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca aatcgttttc
    39421 tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc gcttttatta
    39481 aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc acatgcaatc
    39541 tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat ctttaaatac
    39601 gtatttgata atttatctta acaaccagat aattttattc agcaccgtat tatctatatt
    39661 gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa tcttcttctt
    39721 gttattaata gtgattagga tgatattcct atattgatct ttgataatta gatataattt
    39781 ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac ctagttagcg
    39841 acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca atagcagtaa
    39901 ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt
    39961 acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta taaaatcgaa
    40021 agcctacttg aataaaaaac aagcctgtga atttctagga attgataata atactttaga
    40081 taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag ttcgctttga
    40141 cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt ttcttctcgt
    40201 cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg aggaagtttg
    40261 cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa aaaattcaga
    40321 gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag gcaattagct
    40381 attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga ggagaatgca
    40441 aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc aaaatggctt
    40501 cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa tgtaacaatt
    40561 agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga atatgcaatg
    40621 aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa atcgaaagaa
    40681 tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat tgccgaaata
    40741 ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac cgcgcctaag
    40801 aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc atcagaagag
    40861 ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct tacacttcac
    40921 gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga gacttatgct
    40981 ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata tgctttagat
    41041 aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca ggcaccagaa
    41101 gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt gttgaaacta
    41161 aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc tggagaagta
    41221 aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa aaaaaggcat
    41281 cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac tatagctagg
    41341 gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga tatttctact
    41401 acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga agcatttcaa
    41461 aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt ataaaaaaag
    41521 acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa tattaacgtt
    41581 ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt tcgaccatac
    41641 gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca ggatcaactt
    41701 ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg tatcctccgc
    41761 cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg aaaggttgat
    41821 tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt ttatttacag
    41881 taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta cgacggcctg
    41941 tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag tttgtgatgt
    42001 ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat acgttcaatt
    42061 ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca gaagtttcga
    42121 tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc aatcctcctg
    42181 ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt aatttcactt
    42241 tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt
    42301 tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag
    42361 ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata tatgttgtac
    42421 gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga gtttccgggg
    42481 ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt caatgtcaac
    42541 gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt cttttatata
    42601 aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
    42661 aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    42721 tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc
    42781 aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    42841 aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
    42901 tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg
    42961 cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc
    43021 gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg
    43081 gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt
    43141 acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt
    43201 agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt
    43261 caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat
    43321 gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt
    43381 taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact
    43441 taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt
    43501 tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc
    43561 agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga
    43621 tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct
    43681 atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc
    43741 acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca
    43801 tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct
    43861 gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct
    43921 tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg
    43981 ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc
    44041 agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt
    44101 tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca
    44161 aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca
    44221 taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct
    44281 ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta
    44341 gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct
    44401 gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat
    44461 tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata
    44521 tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta
    44581 taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg
    44641 ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc
    44701 tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct
    44761 cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc
    44821 aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt
    44881 ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct
    44941 gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg
    45001 gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa
    45061 acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta
    45121 tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc
    45181 ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct
    45241 agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc
    45301 tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat
    45361 ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca
    45421 agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta
    45481 ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt
    45541 tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt
    45601 gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct
    45661 tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt
    45721 ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct
    45781 ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag
    45841 gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt
    45901 gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac
    45961 cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta
    46021 gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca
    46081 gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa
    46141 tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat
    46201 cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca
    46261 ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca
    46321 gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga
    46381 tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa
    46441 gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc
    46501 tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc
    46561 atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg
    46621 cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa
    46681 acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat
    46741 ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag
    46801 cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt
    46861 tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat
    46921 caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc
    46981 ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca
    47041 aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag
    47101 caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc
    47161 gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg
    47221 gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta
    47281 caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa
    47341 atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc
    47401 ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat
    47461 gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg
    47521 aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc
    47581 atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc
    47641 cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag
    47701 tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa
    47761 tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc
    47821 tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc
    47881 agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt
    47941 agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa
    48001 agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac
    48061 gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc
    48121 tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat
    48181 ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt
    48241 gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga
    48301 tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa
    48361 aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt
    48421 gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct
    48481 ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt
    48541 tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa
    48601 ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc
    48661 gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca
    48721 gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct
    48781 tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc
    48841 gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa
    48901 acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc
    48961 ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg
    49021 ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct
    49081 tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga
    49141 cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa
    49201 tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca
    49261 atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct
    49321 gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct
    49381 tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct
    49441 gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct
    49501 tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt
    49561 gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact
    49621 tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc
    49681 cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg
    49741 ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa
    49801 tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc
    49861 tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg
    49921 tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt
    49981 tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct
    50041 gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat
    50101 gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca
    50161 aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac
    50221 ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa
    50281 gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct
    50341 cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa
    50401 tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct
    50461 gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc
    50521 cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga
    50581 ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa
    50641 taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc
    50701 cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag
    50761 ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc
    50821 catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa
    50881 aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga
    50941 tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg
    51001 gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg
    51061 cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa
    51121 tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc
    51181 ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat
    51241 caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca
    51301 aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag
    51361 ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga
    51421 agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt
    51481 gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct
    51541 ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag
    51601 cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt
    51661 tctctctcct ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt
    51721 taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt
    51781 cacttcattt tctcctcgca aacgacattc atataccata taaccttcat actcattttg
    51841 aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg
    51901 attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat
    51961 atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg
    52021 attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg
    52081 gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg
    52141 aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg
    52201 taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc
    52261 gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat
    52321 aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc
    52381 tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta
    52441 ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa
    52501 tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg
    52561 atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat
    52621 ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa
    52681 catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat
    52741 gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca
    52801 gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctatt
    52861 aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt
    52921 ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa
    52981 tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg
    53041 gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg
    53101 aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat
    53161 tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt
    53221 ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact
    53281 aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc
    53341 atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc
    53401 aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag
    53461 tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc
    53521 ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact
    53581 aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac
    53641 taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt
    53701 caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt
    53761 caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata
    53821 atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa
    53881 tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc
    53941 ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag
    54001 tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga
    54061 aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa
    54121 gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct
    54181 gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa
    54241 atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca
    54301 ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt
    54361 catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta
    54421 aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc
    54481 aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga
    54541 tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc
    54601 agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt
    54661 gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca
    54721 ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat
    54781 gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta
    54841 gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac
    54901 agatcattga cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa
    54961 atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac
    55021 ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag
    55081 aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca
    55141 agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa
    55201 aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg
    55261 cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt
    55321 gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga
    55381 acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt
    55441 ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt
    55501 ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca
    55561 atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt
    55621 tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc
    55681 atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg
    55741 tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt
    55801 gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg
    55861 taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc
    55921 acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct
    55981 ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt
    56041 gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca
    56101 attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc
    56161 caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct
    56221 tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg
    56281 tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc
    56341 gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt
    56401 acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct
    56461 tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt
    56521 tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc
    56581 tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct
    56641 agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt
    56701 cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat
    56761 ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt
    56821 ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa
    56881 agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt
    56941 tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg
    57001 caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc
    57061 tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc
    57121 cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca
    57181 ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg
    57241 actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt tcttcagctg
    57301 ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat
    57361 accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt
    57421 ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg
    57481 tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat
    57541 attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc
    57601 agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt
    57661 tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc
    57721 taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa
    57781 gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat
    57841 gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag
    57901 tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca
    57961 agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct
    58021 catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac
    58081 ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt
    58141 atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc
    58201 aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc
    58261 tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt
    58321 ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc
    58381 ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca
    58441 atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat
    58501 caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa
    58561 attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc
    58621 tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc
    58681 taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa
    58741 agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc
    58801 aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca
    58861 tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga
    58921 acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa
    58981 atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat
    59041 ggatgaaaca cttctttttg aattggatca atataatatt cctattgtgg taatcggaaa
    59101 agtcggaaac gactacacga acatctactc agtggacacg gataatttcc aggatagtta
    59161 cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc
    59221 gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca
    59281 ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat
    59341 ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga
    59401 tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga
    59461 tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt
    59521 gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca
    59581 actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct
    59641 cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg
    59701 aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg
    59761 tattttagcg aatttttctt attttccatc tgttttagat agctgtggat aactataatg
    59821 tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat
    59881 aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg
    59941 ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt
    60001 cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc
    60061 aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga
    60121 agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac
    60181 ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt
    60241 tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag
    60301 ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt
    60361 ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa
    60421 taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca
    60481 agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg
    60541 cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg
    60601 tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg
    60661 tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat
    60721 gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt
    60781 agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt
    60841 ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat
    60901 aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg
    60961 ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc
    61021 ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt
    61081 ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga
    61141 ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa
    61201 ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac
    61261 ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca
    61321 attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt
    61381 aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt
    61441 ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca
    61501 cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca
    61561 tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga
    61621 agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca
    61681 ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga
    61741 agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag
    61801 cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga
    61861 attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat
    61921 tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc
    61981 ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga
    62041 cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt
    62101 tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg
    62161 tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga
    62221 agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt
    62281 tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc
    62341 gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt
    62401 cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga gtaacaactg
    62461 gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg
    62521 cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat
    62581 tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt
    62641 cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt
    62701 ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt
    62761 tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt
    62821 tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc
    62881 caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga
    62941 aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc
    63001 cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca
    63061 tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac
    63121 ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc
    63181 caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa
    63241 ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga
    63301 ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt
    63361 ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt
    63421 ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt
    63481 ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt
    63541 cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa
    63601 agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcacggcgaa
    63661 ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac
    63721 gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga
    63781 acaacccttc acgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt
    63841 caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga
    63901 atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac
    63961 ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg
    64021 tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt
    64081 ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat
    64141 cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac
    64201 gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac
    64261 ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta
    64321 cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca
    64381 gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg
    64441 gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca
    64501 tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac
    64561 ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg
    64621 tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta
    64681 actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag
    64741 gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat
    64801 cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat
    64861 ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg
    64921 gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc
    64981 cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg
    65041 catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta
    65101 caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat
    65161 cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt
    65221 ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt
    65281 ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga
    65341 aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg
    65401 ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc
    65461 ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt
    65521 cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata
    65581 acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg
    65641 cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt
    65701 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag
    65761 gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac
    65821 gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg
    65881 ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac
    65941 ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg
    66001 cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac
    66061 acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag
    66121 tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt
    66181 ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca
    66241 caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca
    66301 ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa
    66361 gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga
    66421 taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg
    66481 tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc
    66541 ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg
    66601 atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga
    66661 cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta
    66721 atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt
    66781 tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca
    66841 agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt
    66901 tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat
    66961 tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta
    67021 acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga
    67081 tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac
    67141 cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga
    67201 ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca
    67261 ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg
    67321 tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg
    67381 ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat
    67441 tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc
    67501 attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc
    67561 ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct
    67621 tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca
    67681 aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa
    67741 aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa
    67801 agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc
    67861 atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg
    67921 caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac
    67981 agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga
    68041 accaacatca accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga
    68101 taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc
    68161 taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt
    68221 gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca
    68281 atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag
    68341 aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt
    68401 gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc
    68461 aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag
    68521 aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc
    68581 aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg
    68641 gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg
    68701 tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg
    68761 gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa
    68821 atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta
    68881 agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac
    68941 tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag
    69001 atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag
    69061 tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg
    69121 gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg atcatggcag
    69181 caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg
    69241 aactgtatgt caataaagac atggccaaag cattaggcat caatccagac agcatcaaag
    69301 aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc
    69361 tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat
    69421 cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc
    69481 agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg
    69541 tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct
    69601 tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc
    69661 gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac
    69721 aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact
    69781 tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc
    69841 aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc
    69901 taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct ttgctgattt
    69961 aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt
    70021 gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta
    70081 tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc
    70141 ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt
    70201 acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc
    70261 catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt
    70321 tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact
    70381 ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga
    70441 aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa
    70501 gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg
    70561 aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc
    70621 aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt
    70681 ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca
    70741 gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg
    70801 atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat
    70861 gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc
    70921 gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg
    70981 ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca
    71041 gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc
    71101 gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc
    71161 ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa
    71221 ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt
    71281 tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag
    71341 cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag
    71401 caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct
    71461 acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt
    71521 gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga
    71581 cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa
    71641 tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct
    71701 gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta
    71761 tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg
    71821 cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct
    71881 gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa
    71941 gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca
    72001 ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca
    72061 aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg
    72121 tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa
    72181 ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat
    72241 tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag
    72301 catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg
    72361 atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga
    72421 taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt
    72481 aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat
    72541 catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa
    72601 ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag
    72661 ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt
    72721 ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc
    72781 agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt
    72841 ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca
    72901 ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt
    72961 atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt cctccttttt
    73021 atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc
    73081 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
    73141 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
    73201 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
    73261 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
    73321 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
    73381 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
    73441 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
    73501 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
    73561 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
    73621 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
    73681 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
    73741 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
    73801 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
    73861 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
    73921 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
    73981 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
    74041 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
    74101 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
    74161 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
    74221 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
    74281 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
    74341 gctcagattc atcaacttca agcgacagta cagatactac ctctaccaca agtgatagca
    74401 cggatac
//
LOCUS       DACECB010000007        61526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECB010000007 DACECB010000000
VERSION     DACECB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.007"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..186)
                     /locus_tag="IWO04_000518"
     CDS             complement(58..186)
                     /locus_tag="IWO04_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0212154.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IWO04_000519"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IWO04_000519"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0212155.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="IWO04_000520"
     CDS             complement(1673..2728)
                     /locus_tag="IWO04_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0212156.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IWO04_000521"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IWO04_000521"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0212157.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IWO04_000522"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IWO04_000522"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0212158.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IWO04_000523"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IWO04_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0212159.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IWO04_000524"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IWO04_000524"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0212160.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IWO04_000525"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IWO04_000525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0212161.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="IWO04_000526"
     CDS             10490..12049
                     /locus_tag="IWO04_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0212162.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="IWO04_000527"
     CDS             complement(12160..14091)
                     /locus_tag="IWO04_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0212163.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IWO04_000528"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IWO04_000528"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0212164.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="IWO04_000529"
     CDS             complement(15020..15772)
                     /locus_tag="IWO04_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0212165.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="IWO04_000530"
     CDS             complement(15931..16140)
                     /locus_tag="IWO04_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212166.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="IWO04_000531"
     CDS             complement(16151..17479)
                     /locus_tag="IWO04_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212167.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17844..18431)
                     /locus_tag="IWO04_000532"
     CDS             complement(17844..18431)
                     /locus_tag="IWO04_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0212168.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IWO04_000533"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IWO04_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0212169.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /locus_tag="IWO04_000534"
     CDS             complement(20011..21300)
                     /locus_tag="IWO04_000534"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0212170.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21454..22386)
                     /locus_tag="IWO04_000535"
     CDS             complement(21454..22386)
                     /locus_tag="IWO04_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212171.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22543..23202)
                     /locus_tag="IWO04_000536"
     CDS             complement(22543..23202)
                     /locus_tag="IWO04_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0212172.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IWO04_000537"
     CDS             complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IWO04_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0212173.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24243..24533)
                     /locus_tag="IWO04_000538"
     CDS             complement(24243..24533)
                     /locus_tag="IWO04_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212174.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="IWO04_000539"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="IWO04_000539"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0212175.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="IWO04_000540"
     CDS             complement(26820..28175)
                     /locus_tag="IWO04_000540"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0212176.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="IWO04_000541"
     CDS             complement(28199..29368)
                     /locus_tag="IWO04_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212177.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="IWO04_000542"
     CDS             complement(29365..30252)
                     /locus_tag="IWO04_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0212178.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="IWO04_000543"
     CDS             complement(30441..34121)
                     /gene="nifJ"
                     /locus_tag="IWO04_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0212179.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34319..34840
                     /locus_tag="IWO04_000544"
     CDS             34319..34840
                     /locus_tag="IWO04_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0212180.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35539..36104)
                     /locus_tag="IWO04_000545"
                     /pseudo
     CDS             complement(35539..36104)
                     /locus_tag="IWO04_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36283..36939)
                     /locus_tag="IWO04_000546"
     CDS             complement(36283..36939)
                     /locus_tag="IWO04_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212181.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="IWO04_000547"
     CDS             complement(37507..38442)
                     /gene="whiA"
                     /locus_tag="IWO04_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0212182.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38476..39474)
                     /locus_tag="IWO04_000548"
     CDS             complement(38476..39474)
                     /locus_tag="IWO04_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0212183.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="IWO04_000549"
     CDS             complement(39471..40355)
                     /gene="rapZ"
                     /locus_tag="IWO04_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0212184.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40490..41221)
                     /locus_tag="IWO04_000550"
     CDS             complement(40490..41221)
                     /locus_tag="IWO04_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0212185.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41225..42490)
                     /locus_tag="IWO04_000551"
     CDS             complement(41225..42490)
                     /locus_tag="IWO04_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0212186.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="IWO04_000552"
     CDS             complement(42753..45569)
                     /gene="uvrA"
                     /locus_tag="IWO04_000552"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0212187.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="IWO04_000553"
     CDS             complement(45579..47573)
                     /gene="uvrB"
                     /locus_tag="IWO04_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0212188.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47832..49334
                     /locus_tag="IWO04_000554"
     CDS             47832..49334
                     /locus_tag="IWO04_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0212189.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49336..50073
                     /locus_tag="IWO04_000555"
     CDS             49336..50073
                     /locus_tag="IWO04_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212190.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="IWO04_000556"
     CDS             complement(50441..51607)
                     /gene="dnaJ"
                     /locus_tag="IWO04_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0212191.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="IWO04_000557"
     CDS             complement(51758..53587)
                     /gene="dnaK"
                     /locus_tag="IWO04_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0212192.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="IWO04_000558"
     CDS             complement(53638..54201)
                     /gene="grpE"
                     /locus_tag="IWO04_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0212193.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="IWO04_000559"
     CDS             complement(54295..55338)
                     /gene="hrcA"
                     /locus_tag="IWO04_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0212194.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55573..56739)
                     /locus_tag="IWO04_000560"
     CDS             complement(55573..56739)
                     /locus_tag="IWO04_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0212195.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56755..56982)
                     /locus_tag="IWO04_000561"
     CDS             complement(56755..56982)
                     /locus_tag="IWO04_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0212196.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57369..57625)
                     /locus_tag="IWO04_000562"
                     /pseudo
     CDS             complement(57369..57625)
                     /locus_tag="IWO04_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57968..58991)
                     /locus_tag="IWO04_000563"
                     /pseudo
     CDS             complement(57968..58991)
                     /locus_tag="IWO04_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59629..60303)
                     /locus_tag="IWO04_000564"
                     /pseudo
     CDS             complement(59629..60303)
                     /locus_tag="IWO04_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60820..61410
                     /locus_tag="IWO04_000565"
     CDS             60820..61410
                     /locus_tag="IWO04_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0212197.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17075 a        13210 c        10645 g        20596 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    21361 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    21421 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    21481 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    21541 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    21601 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttcattta acacggaact
    21661 aagcagcgca tcttcattac cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    21721 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    21781 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    21841 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    21901 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    21961 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    22021 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    22081 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    22141 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    22201 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    22261 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    22321 tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    22381 cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta gcaaaacaaa
    22441 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    22501 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    22561 gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcctag
    22621 atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca aaggaaattt
    22681 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    22741 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttgac catgattgaa
    22801 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagatact
    22861 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    22921 cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga taaaaaaacc
    22981 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    23041 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    23101 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    23161 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    23221 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    23281 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    23341 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    23401 tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    23461 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    23521 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    23581 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    23641 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    23701 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    23761 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    23821 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    23881 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    23941 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    24001 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    24061 ctgtctccct cttccaacac atttgaagct agttcttcat ccccatataa ccaactgttc
    24121 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    24181 acaaataatc ctgcagcaat cagaactgct gcccaacgtc tcttattcat tctttctcct
    24241 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    24301 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc atcttctacc
    24361 gtaaactcac gagcttcagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    24421 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    24481 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    24541 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    24601 cccattattt tttgacataa aaagagcatt tgacaaagca tttgatatat accaatttat
    24661 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    24721 agataaaaaa caaaagagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tatctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgctg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaatg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagattctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctctggaaaa attgacacag atccctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagatagccg ccaagttctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gcgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gattggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttactcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccatcatt gatatttaac
    27541 ccgtctggat ttgtgcccat ggtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacctc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgattg
    29221 gcaccccagt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatca gttctctacg
    29461 caaaattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaatgtaat
    29521 gctgacatcc ccagtttctt cagagacaac gatggtaatc gcgtcgctga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttcttttgga atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctct ccagtgatat ctgcatctaa
    29761 tgcaattcct gtctcaatat actcatctaa tccagtatgc cgttcgatag taattagcgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat ctgctcgtat ttttcttgag aaaaacgcat aattttcagg
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttctt tcttctgctt caaggtccgt cgttttttct
    30541 tttctagtct taacgggtac atcgatagat tcctctggtt caagtcgttt cttgattttt
    30601 tctaattctc ccgtcagtgc agcatagttg taaaaacgtg ttttagcgtc ttggatagtc
    30661 ttttcaaata aagcttttgc aagatttgga tttgcgttct ctagcgagga aaagcgaact
    30721 tgtttcctca tgaaatcagg catagcagag aaatcaggtt ttttgtagtc caaagtcatt
    30781 gggttcttgt ttttttcgac cattgcaggg ttatatcgat ataatgacca atagccagaa
    30841 gaaacagctt cttttgcttc tttaagcgat tctttcattc caccaagcaa accatgtgtg
    30901 atacaaggcg tataggcaat gattaaagaa ggtccaggat acttttctgc ttcttcaaaa
    30961 gcctttatcg tctgcatttg attggcacct gaagcaattt gcgccacata aacatttcca
    31021 tatgtcatcg ccatcattcc tagatctttc tttgaagcat actttccact agccgaaaat
    31081 ttagcgattg ccgaagctgg tgttgctttt gaggtttgac cgccagtatt ggaatagact
    31141 tcgttatcca aaacaagaat attcacgtct gctccgctgg caagtacatg atcgatcccg
    31201 ccaaaaccaa tgtcgtaagc ccatccgtct ccaccaatca tccattggct gttttttacg
    31261 aatagatcct ttctcttgta tatatcctcc agttcaggga caattgatcg ttcgctttcg
    31321 atcgcagctt gcagttttgc tgctctttgc tgcgttcctt cgctctcaaa catgtgtacc
    31381 aaccagtctt ccatcaattc tcgtagttct tctgatactt ttggcaatac ttctttgatt
    31441 gaacttgcca aagcctcgcg acgagtctgt gccgctagaa gcataccata accaaattca
    31501 gcattatctt ccaataaaga atttgaccat gcaggccctt gaccacattc attggtcgta
    31561 taaggagaag caggagaagc accgccccaa atagaagaac agcctgtcgc attcgcgatc
    31621 atcattcgat caccgaacat ttgtgtcaat aattttacat aaggtgtttc gccacagcct
    31681 gagcaagcgc ccgagaattc aagaagcggt tgattgaatt gtgtagacaa gacagtattc
    31741 tttcctttga ctggatttgc tttttgtctt aatgtcattg caaaagccca attcacagct
    31801 tgttctttct gttcttcata aggcttcatc actaaagctt tttcttttgc gggacaagcc
    31861 tctacacaca gaccacaacc tgtacagtct tccactgaaa cttggatacg gtattttacg
    31921 ccgtctgcgc ctcgcatttc tctcacgata aatccttctg gcgcttcttc catttcctct
    31981 tcatctgcta aaaatggacg gatggcagca tgaggacaaa caaaggcaca ttcattacac
    32041 atcgtacagc gatcactaat ccactcaggt acttctaaag cgacgccccg cttttccata
    32101 gaagccgttc ctaaaggcat ttcgccagtt gtcattccat ttgctacaag tgtaccaaca
    32161 gagagttgat ttccttcctg tgcattcact ggttcaagta tttcttgtat ataaccagtg
    32221 ttccttttat tgattttttc aggcatctcc aacgttctcc aatggtctgg gatcgcgact
    32281 tgatgaagca gttcggtcgt ccgatcgatt gcttgtatgt ttttttctac gatagccatt
    32341 gatttatgtc catagctttt cagggcttct tcttttagca gcggaagcac ctcatcaaaa
    32401 ggaatgatgt cagctaattt aaaaaaagcc gtttccattg ccgtattgat ccggcgtccc
    32461 aaaccgactt cttgggccaa tctcatggca ttgatcgtat agaatctaat gttattttca
    32521 gccaagtaac gttttaattt taagggcaga tgcgtttcta attgttcgtc gctccaaaga
    32581 gtattcaata aaaaaatgcc acctggcttc aatcctttga ccaagtcata gctatgcagg
    32641 taagctggtg tgtgacaggc aacaaggtcc gcatgctcga caagatacgc tgaacgtatc
    32701 ggcgtatctc caaaacgaag atgagagaca gtcagaccgc cagatttttt cgaatcataa
    32761 taaaaatagc cttgcgcata tttatcggtg tgatctccaa taatcttgat agctgattta
    32821 tttgctccaa ctgttccgtc agatccaaat ccccaaaact tggcctggaa tgtttgcggt
    32881 tctgtcaaat ccagactttc ctttggttcc aaagattgat aagtcacatc atccacgatc
    32941 cctatggtaa accgtttttt gcgtatgctt gggtcttttt tcaattcatc aaaaactgct
    33001 gagatttggt cgggagtcac atctttagac cccaatccgt agcgaccacc aataacagcc
    33061 gggcgaagtt cagaatcata taacgcactt tgaacgtcta ataagagagg ttctccacct
    33121 gcccctggtt ctttacttcg atcaagaaca gcaattgttt ttactgtatg aggcaacttt
    33181 tgcacgaatg tttccactgg aaaaggacga taaagatgga tattcaaaaa acctgttttc
    33241 cgtccgtttg cattcaagta atcaatcgtt tgttcaatcg tctgtgcaac tgatcccata
    33301 gaaacaatga cttcttccgc atcgtctgct ccgtaataag tcaccaagtc ataagaagtt
    33361 cctcgtaatc ggttgatctc tttcatatat ttttgaacga tttcaggtaa tttatcataa
    33421 taggggttga tcgtttcccg ttgttgaaaa tggatgtctg gattctggtt cgtaccacta
    33481 acagtcggat gattgggatt catccctcgt ttacgaaatg aagctaattt ctcctgattg
    33541 atcagaggag ctaattcttc atattccaaa acttcgattt tttgaatctc gtgactcgta
    33601 cggaatccat caaagaaatt caaaaaaggt acttctcctt caatagaagc aagatgggca
    33661 acaggtgcta aatccatcac ttcttgtaca ctgctttcag ctaacatagc aaatcctgtt
    33721 tgtcttgcag ccatcacgtc tccgtgatct ccgaaaatat tcaacgcatt tgtcgtcact
    33781 gcccgactag caacatggaa aacacttggc agtagttccc cagcaatctt gtacatattc
    33841 ggtatcatca aaagcagccc ttgagaagcg gtatatgtag ttgtcagtgc accagttttc
    33901 aatgatccat ggaccacacc agctgctcct gcttcggatt gcatttctac tacttttact
    33961 ggttgaccga atacattttc ttttcctgct gctgaccatt gatctactaa ttcagccatg
    34021 gtcgaactgg gtgttattgg atatattgca gcaatctcgg taaatgcata agaaatatat
    34081 gcggctgccg tattgccatc cattgttttc atttttcgca tgattcatca tccttgttca
    34141 tattctctct tattatctgt tccattataa cgtaaaacat tccatttttt cttttattta
    34201 taaaaatatt tcacatatgt ctatttgaat tggtcatcta atttttagac aaacagtcta
    34261 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    34321 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    34381 atcgaatgct cctaaaaaaa tggtcgaatt catcaaccta tcttgtccgt attgccgaca
    34441 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    34501 agtgatcaaa ttgttcgata aagaaaaaga aagcttgcag cgaggaaacg tgatgcaccg
    34561 gtatctcaca atcagcgatg gtcaaaaagc gatcaaagag atcaaacaaa tattcgatac
    34621 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    34681 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    34741 agcacatatt cgatttgttc cgactgtcat cttgggtaaa gaaatctttg atgaatctat
    34801 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtaggac
    34861 aaaagtaaag aacacttttg tctcacgctc taaacaccta taaacggcgg aagcagaagc
    34921 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    34981 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctgagcaaat
    35041 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    35101 tttcatacaa aatttctcaa attcccttaa cgagtaatat ttatgtacgg tgattcacac
    35161 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    35221 tttatgtatg ctgttagttg attagatggt cgtttgttga gctaggtcgt ttattttaaa
    35281 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    35341 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaactc ctaactcctc
    35401 tcatttaata gttgctctct tttttattca gatacatttt tgctgatcac agcctagtat
    35461 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    35521 tggattaata gatttcgatt attcaaagcg attttctatt ggtggattgt gttcccaact
    35581 aacaagacgg attccaaagt cattatataa ctcttcaaga gttaaccctt tcctgttccc
    35641 aatagatgta tctctcccac gctcgatata aaatatggag tttcgaatgc caagacctgg
    35701 caaatctttc cttgttacag ttacactgct actaacttcc ccatataaaa tccagctcct
    35761 ccaaaatgat aatatatttt tcgaccatca tctaactcat gtaacaataa ttgttgtgcc
    35821 ctcttgtcac tcgccaaatg caaacgccac gtgcgatacc atgccatgct acctgcatct
    35881 gcagaaaatc catcaaataa tgcaacaatt gtatcttcta tcacaatcct ttccccatct
    35941 acttcatatt ctaatctaaa atcaaactcc cctcgggtaa tcgcaggtct tggtggctta
    36001 ggacccaata ttattctact taaaaccgca ttcgttccca gatgaaacac agctataaca
    36061 acaataatga ttaatatttt gtttctattt gataaatttc tcattattcg caaatcacct
    36121 ctacattctc acatactagt tcgtaatact cctttaatat caaaatatct actgatcgta
    36181 ataatattcg ctatgaattc ctactactcg tataagaaag gccaaatcat cagcttttac
    36241 ttgctgatga tttggcttgt ttctatttat cgtaattgtt ttctaatcac caaaattgtt
    36301 aacaatcggt tcagatattt cccaattcac tagccttata ttatattgat ttaacaattc
    36361 ttcttcattt attgtatttt gatcaagcgg accaaattgt tctaataatt ctttgtcatg
    36421 taaaatatcc cttctagtaa tgcctctttc cctttcaatg aaagaagcac taggaaaagt
    36481 atgattataa ccattcaacc tatctcccat gtaataatta gcaggtcctg gaaaataaaa
    36541 tatttgctta ttatcactaa cttctaacag caacactaaa tcttctccgc tggctaaacg
    36601 acttgtccat ctacgatatc tccctatgcc ttcgttcatt ccaaatccat caaattcaac
    36661 tattaacgtg tcttctatca catgtaattc ttcattcaat tcatactcaa gtctaaaagg
    36721 aaattctcca tatgtaatct ctggcaatgg tggtgctgga gttctccaaa taaatacaat
    36781 aactgtgaat agaaaaaatc caacgatcga tataaatgtt atgagacctg ctcgaagtaa
    36841 aacccgatat aataaaaatg ctgtatttcg ctttgctata tctagaaaaa taaaaagtac
    36901 tgcaatcata atgatagtaa aagttgtaaa aaaacgcatt tttgccctcc ctttataaac
    36961 atcgattctc aaattctaaa ccaaatcttg gtagttcaag ttcaggtata acgtattcca
    37021 accatacatc tcaattacta tttatgaact tatgattatc actgaataaa tcactccatc
    37081 cccttgttaa aacttctaca ttaattacac ctggttgttt tcgactagct aatccaatga
    37141 acttatcaac catccttatc caccaaccac gagaatcttg accagaagta agaagagcag
    37201 ttctcaagga tgatcgagca gttccttcca gttttttaat taatcaggcc agttatagat
    37261 aaatacttga tcttatctat atcaggactt ctatattaag caaaaattta tgtacaaaac
    37321 taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa acaatagaag
    37381 cctttccaaa ttatgttact ccagtaaaga tggaattata gtttgacgga agaaaagcaa
    37441 gtataacttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
    37501 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
    37561 gtgattaatt cccgattttg atatcgcacc tgagggaatc atttccccta attctttcaa
    37621 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
    37681 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
    37741 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
    37801 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
    37861 taagaaatca gcaatttttt ctgctccttt taaataagaa atatagccat tgcgccgttc
    37921 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
    37981 ttcataaata gaaaatattt ccaaatgata acggctcgtt tccggattat tgactgaacc
    38041 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
    38101 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
    38161 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
    38221 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
    38281 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
    38341 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
    38401 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
    38461 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
    38521 tttgtctcca tcgtggaaga caccaccatc tttcaattcc aaaaagtcgg tggaaatcac
    38581 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
    38641 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
    38701 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
    38761 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
    38821 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
    38881 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
    38941 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtc
    39001 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaagacag catgcaaggt
    39061 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
    39121 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
    39181 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
    39241 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
    39301 catagcgatc gattctctga ttgcaccgct gcttccccca tcatctgcaa cagtcactac
    39361 tgctgtgata tctgctcctt gatttcgcaa acttttcagg ataactggca gaccagtgcc
    39421 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
    39481 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
    39541 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
    39601 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
    39661 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
    39721 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
    39781 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
    39841 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
    39901 tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga tcacggaagc ttgggctttc
    39961 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
    40021 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
    40081 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
    40141 atttcttcaa agaaggaacg agaacgtaaa tccactccta aagccatttt tgtgactttg
    40201 cctgattctt tgattaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
    40261 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accgctcata
    40321 cctgttatga tgactaattg caaattatct gtcataatta cgaactcctc tcacattgtg
    40381 catagtaaaa ttatatcatt ccgggcgtat cattaccagt tttctttttc ttttacaaaa
    40441 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
    40501 atgctgtatc caatgccttg cttctttcag ctagcgctcg ttcaatcgtc cgatctgcta
    40561 aaatagattg ggcatcgatc actggataat ggttatcttc agcaaattca tgcatcaatg
    40621 acaattctgt gcaacctaag ataacttttt cacagtttag gcgctctacc gcttcttcta
    40681 agatttcgta atatagctct tgattcaaat aatccgattc cttgatctca tgatagatca
    40741 gataattgat tttttcttga agaacagtat caggaatcac tgtttcgaat ccaagatttt
    40801 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
    40861 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgaaggatag
    40921 gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg tgcggtgtta caagtcaaca
    40981 caatgaagtt cggctgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
    41041 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
    41101 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
    41161 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
    41221 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
    41281 tatatttcat caacaaccag cgcttgattg accggtcttt ttcataaaac acagtagtac
    41341 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
    41401 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
    41461 ttccataggt cgtttcaaca aagggtttgt taaacactcg atcttctgtg acgaaacgag
    41521 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
    41581 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
    41641 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
    41701 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaac agtggatgac
    41761 caagacacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
    41821 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
    41881 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
    41941 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
    42001 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
    42061 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaagctt actttattga
    42121 tcagtctttc aaataaagaa taatcaatat atggacagat aaaatattct gacagctctt
    42181 gtttatgttg ggagatcaat tctgcatatc catcaccaca agcaatcaaa agatattttt
    42241 tactcttatc agaatatctt tcttttgcca aacgaagcat cgtctcgata aacacagggt
    42301 ctttatcaaa tcctggaata acttctacat tgacaatttt actgtagcgg gtaggggcta
    42361 actgatccga agcatatgct tggcagattt tgccgtatgc ttcattaaaa gaacgtgcca
    42421 taccataaac gttcatgtcg cttcctaata atatagggat aaattcttca ttttcaatac
    42481 tgttcatcat tatctctcac ttactcataa tctcttgttt ttcttttcca actacacaag
    42541 gaatatcata ccataaagaa aaaagcaatc aaaggaaact cctccgattg ctcaattctt
    42601 ttttgaacgg attgaactta taggaagttt ttgtctatac caatcagtgt agaaagaact
    42661 accatgtaat gattctagaa cactgatatt aataaaacaa gcaaaaattc atttttttat
    42721 gtttttttaa ctatctcagc tatctatcct ctttatacca ttattttttt caaatagtga
    42781 cctgtataac tttcttttac gttaatgatt tcttctggtg ttccagtcgc aaggatcgtt
    42841 cctccacctt ctccaccttc tggtcctaaa tcaatgatat aatctgctgt tttgattaca
    42901 tctagattgt gttcaatcac taaaactgtg ttaccagcat ctactaatct ttgtaataca
    42961 tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg ttggttcatc tagtatatag
    43021 aaattctttc cattagagat tttgtgcaat tcactggcaa gtttcatccg ttgtgcctca
    43081 ccaccggaca atgtcgttgc tggttgcccc atcgtcacat aacctaagcc aacatcaaca
    43141 atcgtttgca gtttgcgatg aatctttgga atgtgcttga agaattctac agcatcttct
    43201 actgtcattt ccaaaatatc agcaatgctt tttcctttgt aatgcacttc taatgtttca
    43261 gagttatatc gtttgccatg acatacttca caaggaacat agacatcagg caaaaagtgc
    43321 atttcgatct taataattcc atccccgcga caagcttcac aacgaccgcc ttttacgttg
    43381 aaactaaaac gccctttctt ataaccccgc atttttgcct cgttcgtttg agcaaataac
    43441 ccacggatat catcaaatac acttgtataa gtcgctggat tacttctcgg cgtccggccg
    43501 attgggcttt gatcgatatc gatgatcttt tcgatacttt cgtagccgga aattgtcttg
    43561 aatttacctg gcttagcaga attcttattt aatttttgcg ctaacgattt ctttaagata
    43621 ctattgacca atgtactctt tccagaacca gacacacctg tgactgctac aaattctcct
    43681 aatggaaatt caacactaat atttttcaga ttgttttctg aagcaccagt gatcttgatt
    43741 gctttcccat tacctttacg gcgttctttt ggtacaggga ttgatttttt ccctgaaaga
    43801 tatttccctg tcaaagaatt ttcatcatta gctacttctt caggagtgcc tgcagccacg
    43861 atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa tcagataatc tgctgcccac
    43921 atcgtatctt catcatgttc gactacaacg agggtgttgc caagatcacg cattttcttt
    43981 aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc cgatcgatgg ttcatctaaa
    44041 atatacaaaa ctccagaaag attcgatcca atttgtgtag ccaatctgat tcgctgcgct
    44101 tctcccccag ataatgttcc ggaagcgcga ctcaaagtta gatagtctag tcccacattt
    44161 tccaagaatg aaagtctgtc atttacttct tttaaaatcg gtttggcaat tactttctct
    44221 tgttcagata acgttaagtt tgagaagaaa tctactgctt tttgaataga caactcattt
    44281 gtttgcccga tattcgttcc attgatttga acagatagag cttggggatt caatcgataa
    44341 cctttacatg tctgacaagt taattctgtc atataaagac gcatttgctc acgcgtgaaa
    44401 tcactgttcg tatcatgata acgacgctcg atattcttca ataccccttc gaaagtcgtt
    44461 tctacatcgc gaacgccacc aaaatcattt tcataatgga aatggaaaag atcatctgtc
    44521 ccattcaaga tgatatcctg atgctcttcc ggtaattctt cgaatggtgt atctaaatcg
    44581 ataccgaaac tttcacaagc ctgcgctaac atttgaggat aatactgaga gctgattgga
    44641 ttccatggaa cgatagcacc ttgactaagt gtcttcgttc tatctggaat cactaaatct
    44701 tgatcgactt ctaatttgac acctaaacca tcacagtctg gacaagctcc aaaaggcgca
    44761 ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa atccgcaata aggacaggca
    44821 tagtgttcgc taaacaacat ttcttcttca ccgatcacgt caaccagagc atagccgtct
    44881 gccaatcgca gagccgcctc taaagaatca aatagacgag aacgaatccc ttctttcaca
    44941 atgatccggt cgatgacgat cgcgatatca tgttttttgt ttttctccag ctcaggcact
    45001 tcacttaaat catagatctc accatcgact cgaacgcgga cgtatccctc tttttggatt
    45061 cgttcgaata tttttttatg ttgtcctttt ttctttacca cgatcggtgc caacacttgg
    45121 atctttgtgc gctcaggcaa agccattact tgatctgcca tttgatctac tgattgactg
    45181 gtaatctcga tatgatcgtt gggacaaatc ggatgtccta cacgtgcaaa caacaagcgt
    45241 aaatagtcat tgatttccgt cacagtacct actgtcgacc gaggattttt acttgttgtt
    45301 ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc tatctacgtc cggcttatcc
    45361 atttgtccta aaaactgacg agcataagca gaaaggctct ctacgtatct ccgctgccct
    45421 tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag aaccggacaa acctgtaacg
    45481 accacgaatt tatctcgtgg aatcgttaca tctatatttt tcaagttatg ggcacgtgca
    45541 ccgtgaatcg taattttatc gttagccatg ttgtcctcct actttgaagc ttttaattct
    45601 aaaatcatat ctcgcacatt agcggctgtt tcaaaatcca atgctttcgc cgcatttttc
    45661 atttcttttt ctaatttcat caataacgta tctttttctt ctttcgtcat ttcttcatac
    45721 gagacttgta ccgcttcttt tgtatcatct tccgattctt tcgtaataga aatcaaatct
    45781 cggatttctt taatgatcgt ttttggaacg atcccatgtt cttcattgta cttctgctga
    45841 atcgtacggc gtcttgatgt ttcatccatc gctagacgca tagaatctgt tactttgtct
    45901 gcgtacatga ttactttacc ttcttcattc cgagcagctc gtccaatcgt ttggactaag
    45961 gaacgttcac tacgtaaaaa gccttcttta tccgcatcca agatagcaac aagggaaact
    46021 tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa ctaacacatc aaattcacct
    46081 aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt tgatatcact atgcaaatat
    46141 ttgactttga tccctaattc tttgaaataa tctgtgaggt cttctgccat ttttttcgtc
    46201 aatgtcgtta caaacacccg ctggtctttt tcgactcgtt catttatttc gcctactaga
    46261 tcatcgattt gtcccatgat tggacgaact tcgatcactg ggtcaagcag ccctgtcgga
    46321 cggatgattt gttggataac ggtatctgtt tgttcatgtt catatggtcc tggcgtcgca
    46381 gagacataaa tgatctgatt gacatgtttt tcaaactctt ctaaccgaag tggccgattg
    46441 tctaaagcag atggcaaacg gaaaccgtaa tctactagca tctgttttct cgctcggtca
    46501 ccattataca ttcctctgac ttgaggcatg gttacgtgcg attcgtccgc cacgatcaaa
    46561 aaatcttcag gaaagaaatc caatagcgta taaggcggtt caccttcttt acgtccgtcc
    46621 atatgccgag aataattttc aattcccgac gtgtagccca tttctaacat catttcaata
    46681 tcgtaattgg ttcgctgttc taaacgttga gcttccagaa gtttgttttc atttcgcaag
    46741 acagttaaac gctgttctaa ttccgctttg atattcgcta cggcatgctc catatgttca
    46801 tcattcgtca caaagtgagt cgccgggaag attgccacgt gttccgtttc tcctaaaact
    46861 tcacctgtca gtgcattgac ttctcggata cgttcgacct catctccaaa aaactccaca
    46921 cgtaaggcac gttcatctct tgaagccgga aaaatctcca cgacatcacc gcgcactcga
    46981 aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt ggatactgac taagtcacga
    47041 atcaactgat tgcgatctag ttctgctccc tgtcgaatcg aaactacttg tttttggtac
    47101 tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg caatgacgat cacatcattt
    47161 ctttctaata atgaactagt agccgaatgt cgtaatttgt cgatctcatc atttacactt
    47221 gaatcttttt cgatatacgt atcgcttgaa ggcacataag cttcaggctg ataataatca
    47281 taataagaaa caaaatattc aacagcattg ttagggaaaa attccttgaa ttccccgtac
    47341 agctgaccag ccaatgtttt gttatgggca ataattagtg ttggtttatt cactttctca
    47401 ataagattgg aaatcgtata tgttttccct gttccagtcg cacctaataa gatttgtgct
    47461 tttttcccgc cgactacgcc atccactaat tggttaatcg cttcaggctg atcaccagcc
    47521 ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat ctctctcaat cattcttcgt
    47581 cctccttttc attcattcct tttcatttta cgaccaataa aaagaaaagg aaagcaaagg
    47641 cattccttac taaaaaatta taccacaccg aacatacttt cgccactttt ttatcttatt
    47701 atgcatgtta cgttttataa cataaaatgt tagaaactcg catttttttc attttttcga
    47761 gcttacagtc ttgtctgatt cagcactttt cgcctataat aataaaatat tggaaattta
    47821 ggaggttgat tatgaaaaga aagcatttat ttcactcatt tgttttctta atgacaggac
    47881 ttgtgacact tgcgtttgga catgttgcac aagctgacaa cactacaacg gcagagtcaa
    47941 gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga cgtatacgta attgccagtg
    48001 actctgcttt tgctccattt gaatttcaaa acgcaaaggg cgagtacgaa ggcatcgatg
    48061 taaaattgat ggaaaacatc gccaaactgc aaggatttaa catccgaatg gactaccgtg
    48121 gtttctctgc tgctttgcaa gctttggaat caggacaagc agacggaatg attgccggaa
    48181 tgactgtaac agatgaacga aagaaaagct atgatttttc tgattcttac tttgatagcg
    48241 ggatccaatt agctgtcaaa aaaggagata cttcgatcaa gtcttatgaa gatttgaaag
    48301 gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga atttttaagc gaccatgaat
    48361 ctgaatatgg ctattctatc aaatattttg atgctgctga tcaattgtac gacgggttaa
    48421 aagttggcgc aatcgatgca atgatggatg attatccggt aatcggttat gcgatcaaac
    48481 aaggacaaga attggaaaca ccgatcaaaa aagaatctgg cggaaaatat gcatttgccg
    48541 ttaaaaaagg acaaaatcct gagcttctta atatgttcaa tgaaggattg aaagaattaa
    48601 aaagaacagg cgaatacgat aagattatta aagaatatgt agcggatggt tcagaaactt
    48661 ccagtgagtc ttccactgct gatgaatcaa catttcttgg acttatccaa aacaactatc
    48721 aagcattgtt gaaaggactt tggagaacaa ttctcctaac tttgatctcc tttgctttgg
    48781 ctatagtaat cggtgtgatt tttggattat tcagtgtagc accaatacgc gctcttcgca
    48841 caattgctag tatttatgtc gatatcatcc gaggaattcc aatgatggtt cttgcgttct
    48901 ttattttctt tgggttgcct ggtatcgttg gatttacgat tcctgacttc cttgctggtg
    48961 ttatcacctt gactcttaac gcaagtgctt atatcgcaga gattgttcga ggcgggatca
    49021 acgccgttcc tgtcggacaa atggaagctt ccagaagttt aggtctttct tataatcgga
    49081 caatgcaaaa aatcattttg ccgcaagcga tacgcatcat gatcccatca ttcgtcaatc
    49141 agttcgttat ctcattaaaa gacacaacga tcatttccgc aatcggtgta gtcgaattgc
    49201 tgcaaactgg taagatcatt gttgcacgta caacacaaag tacgtatgtt tacttgatca
    49261 ttgcgatcat gtacttgatt ttgatcacag cattgacaaa attagcaaaa gttttagaaa
    49321 agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc atttagtcaa aaaatacggc
    49381 gataacacag tattaaacga catcaatgtg tccatcaacg aaggagatgt cgtctgtatc
    49441 attggccctt caggttcagg aaaaagtaca tttttgcgtt gcttgaatcg tttagaagaa
    49501 ccttctgacg gtgaaattat tattgacggc gcccacttga tggataaaag cacagatatc
    49561 aacttggtga gacaacatat cggaatggta ttccagcact ttaacctatt ccctcatctt
    49621 tctattttgg aaaacatcat tttagcaccg atggacctga agaaagaatc tagagaagaa
    49681 gcagagaaaa aagcaatcaa attgctgcag acagttggtc tagaagataa aaaagatatg
    49741 tatcctgaaa tgctttccgg tggacaaaag cagcgtgtgg ctattgctcg tgctctagcg
    49801 atgaaccctg acatcatgct ttttgatgag cctacatctg cactcgaccc cgaaatggtc
    49861 ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag gtatgacgat ggttatcgtt
    49921 acgcacgaaa tgggcttcgc taaagaagtg gccaatcgtg tcatgttcat tgacgacgga
    49981 aactttttag aagacggaac gcctcaacaa atctttgaaa atccgcaaaa tgaacggaca
    50041 aaagatttct tgaataaagt attaaatatt taataaaaac caaaaatgcc cggtcgcttc
    50101 atgacgaccg ggcattttta gtttcttgtt ttttcactta actgtaccag ccgcatcgct
    50161 tgttctcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
    50221 ggttcattga cgatatcaag tacatagtcg ataccacctt cagaaactat gatgattatc
    50281 ttattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
    50341 acgtatgatt gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaatag
    50401 aaaatttggg caagtctact ttatattttt actagaaaaa ctacttccgc tttttcccgc
    50461 caaaagcatc tttaaactta tcgaagaaac cgtcttcttg ctgttcttca actttgatgc
    50521 cgccagcttc agcgaatgca cgtaatgctt ctctttgttg ttcatttaga tttttcggtg
    50581 tgatcagttt tactttgaca tgttggtcgc ctgttgcacc accacgtaac ttcggagcgc
    50641 cttttccacg taaacggaag ttcgtacctg tctgtgttcc agcagggatc ttcaatttca
    50701 catctccatg gacagttggt acttggactt catcccccaa agctgcttgt acaaaactta
    50761 aaggcaattc atagtaaatt tcagaacctt cacgatcaaa gatatcactt tcttctacac
    50821 gaaaaaccac atacaaatca ccaaatggtc cgccgttttc tccagcttct ccttgaccag
    50881 ctaaacgcat ttgttgtcct tcttctacac cagctggtac attcacttta actgaatgtg
    50941 ctttcttctc atgtcctgag ccatgacagg ttggacaagg ttctttgatt tctttccctg
    51001 tgccatgaca aacatcacaa gtttgacggc tcatcactcg tccaagtggc gtttggcgtt
    51061 ctacattgat cgtacctgca ccatgacatt tatgacaagt agttggttgt gtgcctggtt
    51121 ttgcaccgtt tccaccacaa gtatgacaga tttcttcccg attatacttg atttcttttt
    51181 ctacaccaaa aatcgcttct tcaaaagaca agtcgaccgt atattgcaaa tcagcacctt
    51241 gacgaggcgc atttggatcg actgaccgac cgcctcctcc aaagaaagag tcgaagatat
    51301 cttcaaaacc accgaagccg ccaccagaga agccgccgaa tccaccaaat ccgccaccag
    51361 ctccaccagc accgtaattt ggatctgtgc ctgcatgccc gtattggtcg tatgcagcgc
    51421 gtttttgcgg gtcacttaaa atctcatatg cttctgatac ttctttgaat ttttcttccg
    51481 catcaggttc tttattgatg tcaggatggt attttttcga aagcttacga taagctttct
    51541 ttatttcatc ttctgatgct ccttttgaga gccctaagac ttcataataa tcacgttttg
    51601 ttgccatagg ctttcctcct attggttctc atcattttca cgtcgcttgt tatcatatca
    51661 tattttgttt gaaacaaaaa ccgatacttc aaactatttt tcaaaagaaa aagccaaagt
    51721 agcaactttg gccttctctc gaaattcagt gaactgatta tttatcgtcg ccatcgactt
    51781 cttcaaaatc agcatcaacg acatcatctg ctccgccttg agctgcttct ggattttctt
    51841 gagcttgttg ttgtgcagct tgttcgtata gtttaacagt caagttttga acgatttcgt
    51901 tcaatgcatc gcgtttagtt ttcatttcct cgatgttgtt tgcttcaaca gcggctttta
    51961 attcgtcacg agctgtttct gcttttttca cttcttcttc gtccactttg ccttctaatt
    52021 ctttcaatgt tttgtccaca gagaatagca atgcgtctac atcattacgt aggtcaactt
    52081 cctctttacg agctttatct gcttcagcgt ttgcttctgc atctttcacc atacgttcga
    52141 tttcttcgtc tgttagacct gaagaagatt tgatcgtgat tttttgttct ttttgtgtac
    52201 ctagatcttt cgcagataca tttacaatcc cgtttttatc aatatcgaat gttacttcga
    52261 tttgtggaat accacgagga gctgctggaa tatctgtcaa ttggaatctt cctagagttt
    52321 tgttgtcagc agccattggg cgttcacctt gtaatacatg gatgtctact gctggttgat
    52381 tatctgccgc agttgagaat acttgtgatt tacttgttgg aatcgttgta ttgcggtcaa
    52441 tcaatttagt gaacactcca cccattgttt cgatacctaa tgacaatggt gtaacgtcaa
    52501 gtaaaacaac atctttcaca tcaccagtga tcacgccacc ttggatagca gcacccatag
    52561 caactacttc atcagggtta actgatttgt ttggttcttt tcctgtttct ttacgtactg
    52621 cttctacaac tgcagggata cgagtagatc caccgactaa aataacttcg tcgatttctg
    52681 attgtgacag acctgcatct tttaacgctt gacgtactgg tacttttgta cgttctacta
    52741 agtcagctgt taattcatca aattttgcac gtgtcaatgt catttccaaa tgcaatggtc
    52801 cagattctcc tgctgtgata aatggcaagc tgatttgtgt gcttgatacg ccagataaat
    52861 cttttttcgc tttttcagca gcgtctttca agcgttgtaa agccattttg tcttgagaca
    52921 agtcaatgct gttttctttt ttgaattctg ctaccatgta gtcgatgatt ttgttatcaa
    52981 agtcgtcccc acctagatgg ttgtcgccag ctgttgacaa tacatcaaag acaccgtctc
    53041 ctagttcaag gatagatacg tcaaaagtac cgccaccaag gtcgaatact aagatttttt
    53101 cgtcgcgatc tgttttatct aaaccatagg caagagcagc tgcagttggt tcattgacga
    53161 tacgttctac ttctagacca gcgatcttac cagcatcttt tgttgcttga cgttgtgcat
    53221 cattaaagta agctggcaca gtgataactg ctttttcgac tttttcacct aaatagtctt
    53281 ctgcgaatcc tttgatatat tgaaggatca ttgctgagat ttcttgtggt gtatatgatt
    53341 taccttctac atctactttg taacctgctt caccgatatg gcgtttgatt gatgaaatcg
    53401 tatttgggtt tgtcacagct tgacgttttg caacttcccc tacttggatt tctccatttt
    53461 tgaatgatac aacagaaggt gttgtacggt ttccttctgg gttagcgatg atttttgctt
    53521 cgtttccttc caatactgct actgcagagt tagtcgtacc taagtcaata ccgataattt
    53581 tactcataat taacgtctcc ttctttaatt gaaagtgtaa gttttatatc cgcacaatta
    53641 ttgtgcaaca atgaccatag ttggtcttaa cacgcgatca tgtaatttgt agccttcttg
    53701 tagtacttct acaatcgtat cggctggtgt atcttcagtc gctggaacag tttgtactgc
    53761 ttgatgaaga ttaggatcaa aggcttctcc tttagcaggg attttttcaa ttccttcttc
    53821 ttccagtgcg ttacgcaaac tttccaagac catttccacg ccttttttca aacctgcccc
    53881 ttgatcatct gacacttctg tggctaatgc acgttccagg ttgtcaattg atggaagtaa
    53941 ttttttggct aaatcttgtg aacggtattt ttgtaattgc tcacgctcat tttttcctcg
    54001 atttgccata tttgcgattt cagcacgagc ccgcaagtat ttatcttcca tttcttctaa
    54061 ctcagctttt aatttcgctg tttcgaattc ttccgcttca gcttctgaaa tacccgaatc
    54121 ttctgcagct tcgacatcga tttccggttc tggctgagca tctgtcattt ctttatctag
    54181 ttcttcttga ttttctttca cgatacagtc ttcctttcat tattcagtca gctgtttgat
    54241 tgctcttact aagaaagaga gaacgatcag ctgcaaatag cttcgttatt cacattacga
    54301 gtttgacaag tcaagcgagc ggtagtaatc agcaagttta gctgctagtt ctcttcgata
    54361 aacatcgact agaccgaaca tccgagaata aggcatacta gtaggcccta acaaggctat
    54421 tgtacctctt ccatgaccat ctatctcgta acttgcttga atcatactca tattctgcaa
    54481 taaatcattg cctaattcag caccaatctt gatatgaata ttgctatcca tcggagtgat
    54541 catttgtgta agtccgtctg agtctttcat aaaagaaaac atcgatttga actgatcaag
    54601 atcttgacgg ggctcataat ccaagagatt catttgtcca ctgacataaa ccttttcttc
    54661 aaaaacatga ccaagaacgg tgtcaaataa ctgagacatc ccttctgtcg tctggaaata
    54721 tttatgaaga atcatcggaa tctccgtacg aagtttatgg tacaccgtca tcagaggatc
    54781 tccgactaga cggtcgttta caatctgtac cattttttct agttcctgac ttcccatata
    54841 ttcgggaaga gtgaacactt ggctttctac attccccata tctgttacga cgatagcaat
    54901 aatctgtcga ttattcaagg gtacgatacg aaagccagtc agtcggcggt cttttatttc
    54961 aggtcccaat gaaaaagtcg tatagcttgt cagctcagat aatatctcag cagattgctt
    55021 aataatctca ttgatttcat gaaattctct gcctagagac tgacgaatca gcagcaaatc
    55081 atcttctgct acgcgagcag gttttaacaa atgatccacg taataacgat agcctaaagc
    55141 agatggaatc cgcccagaag aagagtgagt cttcaacagc aatccttcat cttctaatgc
    55201 tttcatatca ttgcgaatcg ttgctgagct ggccttgact ccctcagaca ttaatgtttt
    55261 ggaaccaacg ggcacgccac tactcgtata gtgttggatg atcagacgta aaatgtcgtt
    55321 ttgtcgcttt gttaacatct tcctcacctc tcattagcac tcatatattc taagtgctaa
    55381 ttcacttata aatatagcaa gaagcccttc tcctgtcaag agaaagactc cattttttag
    55441 cactctaaac acaagagtgc taattctaaa taattgatgt aaatccagtt atatcaatac
    55501 ttcaatacct cattgtcaaa ctttctaaac tttttctttt caagtttcga taaaaaacaa
    55561 atattcttca aactattttt caaataaaaa gcgctcaaac acattgttcc ccaaaaaagt
    55621 ccctcttggt gtcaaataaa cacggtcttc ttcattaaca agtaaccctt cttgctctaa
    55681 ttcccttagc acatcaccgt acaccgattc aattgttttt tgatagcgat catagaaatg
    55741 ttttttagta atccctgttt tttttcttag tcctaagaac atttcttctt ctatttgatt
    55801 ttttcgtgtc aatttttctt cttccaatac cgggcgttca tttgctcgta atgggcgtaa
    55861 atagtgttgg attggtcctt tgttgcggta acgaatatct ccaagataac cgctagcacc
    55921 agctccaaac ccaaaatagt ggtcattgtt ccagtacatg aggttatgtt gagattcatg
    55981 tccggagagc ccaaagttac ttatttcata ttggtgacgt ccatgctttt ccattgcttc
    56041 aatcgtctct tcaaacattc tggtttccgt ttcctgatca ggcagttcga gtcttccttg
    56101 tcgtacccag ttcatgaaca ttgttttgtt ttcgaggatc aatgagtaca aagaatagtg
    56161 aggcagatcc aaagccaaag ctttttccaa agtatcccga tatccttcta gtgtttgtcc
    56221 cggtaatgca taaatcaaat cgatgcttac attggtaaaa ttttctgctt ctaaaaattg
    56281 cattgtttga tacacatctt ctgcagtatg tttacgtcca atttttttca ataagcgatt
    56341 gtcaaatgtc tgcactccca tggataaacg attgacgcca tagtttttca tcacttgaag
    56401 tttttcacgg gtcaaatcac caggattcgc ttctacagta aactcattac cattttgaaa
    56461 aggcaggatc tctctagcac ctgataatag tcgatcaagt tgtttagcag ataaagaagt
    56521 tggtgttccg cctcctacat aaagtgtttc caaattcgct gtaggatgac tttccagcat
    56581 taatctcatt tcattcagca gcatttccac atactcatct actggctgcc cttctaaaaa
    56641 aactttatta aagtcacagt aatagcagat atgctcgcaa aatgggatat gaatgtacgc
    56701 agaaaccttg ttttctgcgg gatttattgt ttctatcatt gaaattcttt ctccttacgt
    56761 cgaaaagtcg tgcctgtttt cgaccttctt aaaatcgcgt tcaagcacca actcattgaa
    56821 ataagccgca tatttagaat caaggaaacc tagctctaaa aatgcttgct tattgtcatc
    56881 ggagctacac ggtgctttta cagcctctcg ataaacggta atcgcaaagc tgaccttcgg
    56941 cattaagtca aaatctgcaa aaatatgaaa agctattttc acagattcct ccttaagtgc
    57001 tcagaccaca acgctttgct tacaacctct catatacgca tactaaacgt atttggcgaa
    57061 caaatccact aaaaaatcca agaatcgact tcttggatga tttttttaca ctcatgattc
    57121 ttcctaaaca attttgtcct tattttattc ataaaaaaat ctagtcaaat ctctcacttc
    57181 aagagtttta actagattcc aattattatt tttttgtaaa aataaacagt ctcttaatca
    57241 tagacatttc ttcggtgccc tacttgaata gcgataacaa cgaattgatt atcttctatc
    57301 tctaccaata cacgtatttg ccaatcagag ctgcttctga atttcgggcg tgaattttaa
    57361 acggcaggtc attccagccc accaaattca ttaaggattc ttccatgaat actgttgttt
    57421 tcccacttct tatccaatgt ttatgggcaa tttcagccgt ttgacgattg tattcatctt
    57481 ctagttgttc catcgaatag ttttttagaa gttcagataa agtaatccca taaaattcag
    57541 ccatataaga aagccattct tttttggcgt cacttacacg aaccgtaatc atagccctat
    57601 aattgcctct cttaaagaat ctcatgcatt tcctgatgtg attttagaaa aatttacttg
    57661 ctaaaaaact gtgaaaatgt aggagctagc atccttgtag taaatgatcg agctctttat
    57721 tatacattaa aaaagttcag aaattttgat gaagtagcca actacttttt catgagtaaa
    57781 gcaacactag aaaaagaatc ctccagtact tagaatttac tggaggatgt acttttgaag
    57841 aagcctatag gttatttaaa aaatattact acaggaagga acgagaatta cttcgttact
    57901 tataatagaa cttagtctta tatagaaggc atagctcaca ttagcttatt tgtcctataa
    57961 aaataaatta cggtatttta gaaaagctat tttctctttt ttcaacgatc ttcagcaatt
    58021 tttctttatc gtcaggtaac tcaagttcat gaattctagc atgacaattg ggacataacg
    58081 cgatacagtt ttcaatcaca tcttttccac cttgcgacaa atattctata tgatgacaat
    58141 gtaaataagg tacaccgtct tttataaatg gccctttttc ttggcaaagt tgacaaatac
    58201 cttttgcaag tcgtttaaca tattcagata cgaagggatt acgtttatat atttttgttt
    58261 ttgagaagca aaactcctct tcgttaacat attttgcagc cattttttta ttataattac
    58321 taacttttct tgcttcttcc tctaatttag cattagataa attacgaata tgtctagttt
    58381 gattttcttc ctggttatat atctttatct tcaatcaatt gctcttgatt tattaaagct
    58441 aatggaaatt tatatacttt tctgctgttg cccgaactat ctttttcatt aacagtatat
    58501 atttgtcctg ctaaatatac ttctcccaga tatgtgtgtt cttttggttt aaatgtttca
    58561 aataaatgaa tctttacaaa attacttttt gaattggcta aaactttatt ttgattctta
    58621 tccaatactt gatccccttt tagccccata ccggtatatt gtaaaatacc atttaaccac
    58681 ttatctttat aaacattact tcctgtttga tcactaaaca gtacaagagt attagtttga
    58741 tgagatcttc tcattccacc ctgtttggaa catttaaact tctctgaaat ttctttgtta
    58801 gtatatactc taccaacttc taactcacct aatatgtttt tagaccaatt ttctcgccat
    58861 gtaataatct tatcaagatt aaacatttct ccctcttttg gcatgccatt tctaattaaa
    58921 ttagctaata caccagtaga aatatctaat ttttttagta attctttctt agtaatcaat
    58981 gttggattca taacaatgca gctcctaatg atatcaatat aattgcatta gataacttga
    59041 aaaatcgaaa aaaactcatt ttgtgtcaag ttatctgtaa tatataaaaa tatcattatt
    59101 atacctcatc tatgtgaatt tttactattc ttaccttagc atattcttta taggtatatc
    59161 agagctaatc aaactaaaaa atacccgaag tcttcttatg taaaaacacc ggttattact
    59221 taaatatgac ttaggtaaaa caatgagtga tccaattata aaattttatg gcgaataatt
    59281 tagtaagcgc tacatattga atgatttaga aattttgaaa aagtagctag ttccttttat
    59341 accagaaaag taaacctaat aaaaaacaca actaattttt agctgtgttt tttaagttac
    59401 aataattatc tatttaatag tcttaattca ttaaaataaa aaactcaact ttacaaggat
    59461 atggtttaaa aaatttattt atccaaccca actagttttc gctgcttacg atttgcttca
    59521 tctgctacgt aattcattac tgttttaaat tctttcgctg aggcgttttc tgataaatca
    59581 gcgatacttt tattatactt cttcacgatt tgttgtactt gttctttctc agtcatgtaa
    59641 ttcctcctcc aactcttttg aagtatattg tattggtagt actaattgaa tattatctgc
    59701 tgcataatgc acttcagaaa tgtcgttttg ataatcctta atcattatgt gaaggactac
    59761 ttcaggatgt ctatttttga ctttttcaat attctctctg gacttaaaaa acgcattgat
    59821 aaacatttct tttggcacat gacgtccttc agctagttca cgttttcgag tgaaatccca
    59881 ggcaactttg ggatcttggt aaacatagaa aatcgtaacg cgaaagttat ttttaagggc
    59941 tcgaataata tttttctctg cactaaggat agcaaaagta tcctctagta tgaaagaata
    60001 gccatgttcc agtacatatt ttaatgattg ttcaactaac caagaagaag ctttctgaaa
    60061 aacacttgag ttctttccat tgtattctgg aaattgtgct ctaaaatagt ccgcatcaat
    60121 cacaacatgg ttgtcataat tttaagctaa acctagtgca acctctgttt tacctgctcc
    60181 tgggcttcca gccatgaata ccgcatcttt ttcatcatca gggatttttc ccttgatgat
    60241 tgtttcaata aatttatctt tattgaccct agcgtaatct aaatacttgt ctttaggttc
    60301 caagattgca ccttctttcc ttcttctata ttcatactaa ttatacgata tttatataaa
    60361 aaatataacg gtcattttaa ccatattaac atttagtcac agtaatagca gatatgctcg
    60421 caaaatgaga tatgaatgta cgcagaatcc ttgttttcta cggcattcgc ttttttttca
    60481 ttggactagt tatatatttc tagggttgtt ttcatgtctg agtgcccgat atgatttatt
    60541 tttcgtttct ataattactt tttgccttcc agtttctttt aagattgtac tcaagcaagt
    60601 gggaattggt tcttttgtca tccatgtatg caaaatgtta taatgctggt aaacaaaaat
    60661 gattacctgt catttttatt tagttcgtat ttaattaagt tgactgtctg tatactctgt
    60721 ctaaaactta agaattgttg ttttcgctgt ccaaatttaa tgaatgtctg tatgttaaaa
    60781 ctgttgtcct tgtcatagat agtaaaggag agcttgaaaa tgaaaactgc gattacgttt
    60841 ctattagagg aatatgccga ttgggaaggt gcttatatta ctagcacact aaaccaaaaa
    60901 gagggatggc aaaacaaaac tgcttctatc caatcagagg tcacttccat tggaggatta
    60961 aaaacaaaaa tcgattactt aatcgaacaa atacctgaaa aagttgacct ttttattatg
    61021 atcggtggca attcatggga cataaaaaat catcatttat atcaaataat cgataaattg
    61081 ttaaaaacag acgtaccagt agctgctatt tgtggggcag ttgattattt agctcagagc
    61141 ggattattgc aaggttacaa acacaccgga aatgcgcaat ttccttggtt agaatcggaa
    61201 aattatcaga ataaagaaga ttttatagaa caacaagctg tgaaagatcg taatttgatt
    61261 actgcgaatg gaacagctcc tatagaattc acagacttga ttcttcaagc agtccatttt
    61321 gaaacagaag aaataagaga aaaaaagatt cgtctataca aacttggatt ctatgattac
    61381 tataaaaagt acggagatcc tttctcctaa tcattctctt caattaaata ctccttatca
    61441 atagtatgac aagtaatttt actgatggtt atttgtcaca ctttttctga catttatcaa
    61501 agaagataca ctctatttat attcat
//
LOCUS       DACECB010000008        57516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECB010000008 DACECB010000000
VERSION     DACECB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.008"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWO04_000566"
     CDS             complement(61..2424)
                     /locus_tag="IWO04_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0212198.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IWO04_000567"
     CDS             2872..3093
                     /locus_tag="IWO04_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0212199.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IWO04_000568"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IWO04_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0212200.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IWO04_000569"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IWO04_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0212201.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IWO04_000570"
     CDS             complement(5894..6205)
                     /locus_tag="IWO04_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0212202.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IWO04_000571"
     CDS             complement(6202..6495)
                     /locus_tag="IWO04_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0212203.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IWO04_000572"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IWO04_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0212204.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IWO04_000573"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IWO04_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0212205.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IWO04_000574"
     CDS             complement(8333..12685)
                     /locus_tag="IWO04_000574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0212206.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IWO04_000575"
     CDS             complement(12897..14606)
                     /locus_tag="IWO04_000575"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0212207.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IWO04_000576"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IWO04_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0212208.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IWO04_000577"
     CDS             complement(16103..16903)
                     /locus_tag="IWO04_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0212209.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IWO04_000578"
     CDS             complement(16900..17712)
                     /locus_tag="IWO04_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0212210.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IWO04_000579"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IWO04_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0212211.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IWO04_000580"
     CDS             complement(18467..19189)
                     /locus_tag="IWO04_000580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0212212.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IWO04_000581"
     CDS             complement(19325..20206)
                     /locus_tag="IWO04_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0212213.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IWO04_000582"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IWO04_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0212214.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IWO04_000583"
     CDS             21446..21925
                     /locus_tag="IWO04_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0212215.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IWO04_000584"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IWO04_000584"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0212216.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IWO04_000585"
     CDS             23226..24011
                     /locus_tag="IWO04_000585"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0212217.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IWO04_000586"
     CDS             complement(24357..26048)
                     /locus_tag="IWO04_000586"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0212218.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IWO04_000587"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IWO04_000587"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0212219.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IWO04_000588"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IWO04_000588"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0212220.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IWO04_000589"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IWO04_000589"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0212221.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IWO04_000590"
     CDS             30332..31033
                     /locus_tag="IWO04_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0212222.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IWO04_000591"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IWO04_000591"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0212223.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IWO04_000592"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IWO04_000592"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0212224.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IWO04_000593"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IWO04_000593"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0212225.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IWO04_000594"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IWO04_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0212226.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IWO04_000595"
     CDS             complement(34528..35508)
                     /locus_tag="IWO04_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0212227.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IWO04_000596"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IWO04_000596"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0212228.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IWO04_000597"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IWO04_000597"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0212229.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IWO04_000598"
     CDS             38266..38433
                     /locus_tag="IWO04_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0212230.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IWO04_000599"
     CDS             complement(38495..40306)
                     /locus_tag="IWO04_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0212231.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IWO04_000600"
     CDS             complement(40303..40668)
                     /locus_tag="IWO04_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0212232.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IWO04_000601"
     CDS             complement(40831..41226)
                     /locus_tag="IWO04_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0212233.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IWO04_000602"
     CDS             complement(41244..42206)
                     /locus_tag="IWO04_000602"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0212234.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IWO04_000603"
     CDS             complement(42206..42418)
                     /locus_tag="IWO04_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0212235.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IWO04_000604"
     CDS             complement(42439..43137)
                     /locus_tag="IWO04_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0212236.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IWO04_000605"
     CDS             complement(43157..43699)
                     /locus_tag="IWO04_000605"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0212237.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IWO04_000606"
     CDS             complement(43831..44835)
                     /locus_tag="IWO04_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0212238.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IWO04_000607"
     CDS             complement(44832..45821)
                     /locus_tag="IWO04_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0212239.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IWO04_000608"
     CDS             complement(45818..46624)
                     /locus_tag="IWO04_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212240.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IWO04_000609"
     CDS             46790..47746
                     /locus_tag="IWO04_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212241.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IWO04_000610"
     CDS             complement(47823..48341)
                     /locus_tag="IWO04_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0212242.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IWO04_000611"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IWO04_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0212243.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IWO04_000612"
     CDS             48806..49252
                     /locus_tag="IWO04_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0212244.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IWO04_000613"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IWO04_000613"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0212245.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(51667..52641)
                     /locus_tag="IWO04_000614"
     CDS             complement(51667..52641)
                     /locus_tag="IWO04_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0212246.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(52977..53064)
                     /locus_tag="IWO04_000615"
     tRNA            complement(52977..53064)
                     /locus_tag="IWO04_000615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53030..53032),aa:Ser,seq:cga)
     gene            complement(53209..53775)
                     /locus_tag="IWO04_000616"
     CDS             complement(53209..53775)
                     /locus_tag="IWO04_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0212247.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            54030..55361
                     /locus_tag="IWO04_000617"
     CDS             54030..55361
                     /locus_tag="IWO04_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0212248.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            55327..55677
                     /locus_tag="IWO04_000618"
     CDS             55327..55677
                     /locus_tag="IWO04_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212249.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(55769..56188)
                     /locus_tag="IWO04_000619"
     CDS             complement(55769..56188)
                     /locus_tag="IWO04_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0212250.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(56189..56533)
                     /locus_tag="IWO04_000620"
     CDS             complement(56189..56533)
                     /locus_tag="IWO04_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0212251.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            56883..57479
                     /locus_tag="IWO04_000621"
     CDS             56883..57479
                     /locus_tag="IWO04_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0212252.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        16181 a        12116 c        10052 g        19167 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    51661 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    51721 atgacgatcc aagtcttctt tatttatatt tacagcagta atttgactgt cttcatacgt
    51781 acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta
    51841 ttttccttct ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa
    51901 aaattgactg atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt
    51961 ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc
    52021 acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga
    52081 tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa
    52141 cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga
    52201 tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc
    52261 gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg
    52321 ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc
    52381 tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt
    52441 agcctcataa tagagtggat agttggatac cccagctgct attcctatca ttgcataatg
    52501 tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac
    52561 tacttcatta taagacatct cataatcaaa attccttcca aaataatggt ctttcgttgc
    52621 ataagtgatt gcggtacaca tcgttttccc tcctaaaaca aatcgttttt acacttttat
    52681 cttacatcta gtattttttt ttatcaaaaa aaaggccaaa gaagattgat ttactagcat
    52741 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    52801 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    52861 ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg
    52921 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    52981 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
    53041 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
    53101 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
    53161 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
    53221 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
    53281 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
    53341 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
    53401 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
    53461 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
    53521 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
    53581 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
    53641 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
    53701 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
    53761 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
    53821 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
    53881 tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
    53941 ccttacattt ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata
    54001 gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc
    54061 atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg
    54121 ttttttttct ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc
    54181 taactattct tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg
    54241 gaagcgcatt cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc
    54301 acaaggtgat tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt
    54361 tttttggcaa aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg
    54421 tatttgtcaa taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat
    54481 caccggtcgt tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat
    54541 tggggaattt agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt
    54601 tagttttcca tcgtggattc acactttata atattggatt cacctctggg ttgattgcga
    54661 tgacttttac tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt
    54721 tcaatgaata tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg
    54781 gaattggttt ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat
    54841 ctggaaaatt gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca
    54901 tgggcttagt tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg
    54961 gacctgtcat cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa
    55021 aaaacagttt tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg
    55081 agatcaccag tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa
    55141 gcggttttta cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc
    55201 ataacgtcag cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt
    55261 tcgttgcagg tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa
    55321 agacacttgg aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat
    55381 tcccatgaaa taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata
    55441 gagatacagg gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg
    55501 aacaatcctc gggaccctag tatggaaatc tactatcaag aactattagg taatccccga
    55561 catgatcggg aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat
    55621 gcccagccga ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc
    55681 tattttctat ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc
    55741 tactttcagg agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat
    55801 ctgttacagg ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac
    55861 tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag
    55921 ccattaatcg tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa
    55981 gtaagatttg atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag
    56041 ttacattacc tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt
    56101 gcccggtaaa tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat
    56161 tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt
    56221 ctaaatagat ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa
    56281 aagctaaatg ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa
    56341 tcttcagatg agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga
    56401 tgatactacg ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat
    56461 catcatttaa ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt
    56521 gtttttctgc catttctctt attctccctt cacgactgtt aggtgattca actatggttc
    56581 taaggcaact ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc
    56641 tgtccaatgc ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt
    56701 ttattttaaa aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa
    56761 tcttttatac ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc
    56821 gcagttagtt gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa
    56881 taatgaatat tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc
    56941 tatcaatcgc gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt
    57001 taagaggatt agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa
    57061 tcctgcaagt agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga
    57121 tatttggccc ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta
    57181 tcatttttct gctagggcgt cgttatggaa agccattcat actttccatg attagcgata
    57241 agacatataa caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg
    57301 ctctagctat ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga
    57361 caaacatgtc agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt
    57421 tcttatatag tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat
    57481 atttattttg taaaattagt cattgattga caatgt
//
LOCUS       DACECB010000009        56307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECB010000009 DACECB010000000
VERSION     DACECB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.009"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWO04_000622"
     CDS             330..1175
                     /locus_tag="IWO04_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0212253.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWO04_000623"
     CDS             1456..2676
                     /locus_tag="IWO04_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0212254.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWO04_000624"
     CDS             2673..5393
                     /locus_tag="IWO04_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0212255.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWO04_000625"
     CDS             5377..6321
                     /locus_tag="IWO04_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0212256.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWO04_000626"
     CDS             complement(6390..7400)
                     /locus_tag="IWO04_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0212257.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWO04_000627"
     CDS             complement(7580..7924)
                     /locus_tag="IWO04_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0212258.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWO04_000628"
     CDS             complement(7917..8351)
                     /locus_tag="IWO04_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0212259.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWO04_000629"
     CDS             8489..9226
                     /locus_tag="IWO04_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212260.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWO04_000630"
     CDS             9219..10430
                     /locus_tag="IWO04_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212261.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWO04_000631"
     CDS             10637..11416
                     /locus_tag="IWO04_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0212262.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWO04_000632"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWO04_000632"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0212263.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO04_000633"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO04_000633"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0212264.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO04_000634"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO04_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0212265.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWO04_000635"
     CDS             15018..16067
                     /locus_tag="IWO04_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0212266.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWO04_000636"
     CDS             16064..16342
                     /locus_tag="IWO04_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0212267.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWO04_000637"
     CDS             16317..16787
                     /locus_tag="IWO04_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0212268.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWO04_000638"
     CDS             16804..17106
                     /locus_tag="IWO04_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212269.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWO04_000639"
     CDS             17123..17494
                     /locus_tag="IWO04_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0212270.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWO04_000640"
     CDS             17466..17825
                     /locus_tag="IWO04_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212271.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWO04_000641"
     CDS             17997..19004
                     /locus_tag="IWO04_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0212272.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWO04_000642"
     CDS             19029..20213
                     /locus_tag="IWO04_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0212273.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWO04_000643"
     CDS             complement(20650..22233)
                     /locus_tag="IWO04_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0212274.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWO04_000644"
     CDS             complement(22811..22954)
                     /locus_tag="IWO04_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0212275.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IWO04_000645"
     CDS             complement(23177..23656)
                     /locus_tag="IWO04_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0212276.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IWO04_000646"
     CDS             complement(23729..24013)
                     /locus_tag="IWO04_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212277.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IWO04_000647"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO04_000647"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IWO04_000648"
     CDS             complement(24357..25643)
                     /locus_tag="IWO04_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0212278.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IWO04_000649"
     CDS             25781..26452
                     /locus_tag="IWO04_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0212279.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IWO04_000650"
     CDS             26418..26906
                     /locus_tag="IWO04_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0212280.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IWO04_000651"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IWO04_000651"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0212281.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IWO04_000652"
     CDS             27867..28631
                     /locus_tag="IWO04_000652"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0212282.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IWO04_000653"
     CDS             28864..31077
                     /locus_tag="IWO04_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0212283.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IWO04_000654"
     CDS             31088..31534
                     /locus_tag="IWO04_000654"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0212284.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IWO04_000655"
     CDS             31705..32964
                     /locus_tag="IWO04_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0212285.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IWO04_000656"
     CDS             complement(33053..34000)
                     /locus_tag="IWO04_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0212286.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IWO04_000657"
     CDS             34394..35686
                     /locus_tag="IWO04_000657"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0212287.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IWO04_000658"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IWO04_000658"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0212288.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IWO04_000659"
     CDS             37579..38454
                     /locus_tag="IWO04_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0212289.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IWO04_000660"
     CDS             38720..40603
                     /locus_tag="IWO04_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0212290.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IWO04_000661"
     CDS             complement(40678..40962)
                     /locus_tag="IWO04_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0212291.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IWO04_000662"
     CDS             complement(40952..41686)
                     /locus_tag="IWO04_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0212292.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IWO04_000663"
     CDS             41815..42438
                     /locus_tag="IWO04_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0212293.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IWO04_000664"
     CDS             42630..43751
                     /locus_tag="IWO04_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0212294.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IWO04_000665"
     CDS             43758..46886
                     /locus_tag="IWO04_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0212295.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IWO04_000666"
     CDS             46905..50099
                     /locus_tag="IWO04_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0212296.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IWO04_000667"
     CDS             complement(50245..50952)
                     /locus_tag="IWO04_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0212297.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IWO04_000668"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IWO04_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0212298.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IWO04_000669"
     CDS             52447..53100
                     /locus_tag="IWO04_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0212299.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IWO04_000670"
     CDS             53326..54324
                     /locus_tag="IWO04_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0212300.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..>56307
                     /gene="pepF"
                     /locus_tag="IWO04_000671"
     CDS             54500..>56307
                     /gene="pepF"
                     /locus_tag="IWO04_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0212301.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18531 a         9716 c        11671 g        16389 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccgc cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttata
//
LOCUS       DACECB010000010        55286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECB010000010 DACECB010000000
VERSION     DACECB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.010"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(149..2302)
                     /locus_tag="IWO04_000672"
     CDS             complement(149..2302)
                     /locus_tag="IWO04_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0212302.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2596..3444)
                     /locus_tag="IWO04_000673"
     CDS             complement(2596..3444)
                     /locus_tag="IWO04_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0212303.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="IWO04_000674"
     CDS             complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="IWO04_000674"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0212304.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5945..6124)
                     /locus_tag="IWO04_000675"
     CDS             complement(5945..6124)
                     /locus_tag="IWO04_000675"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0212305.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="IWO04_000676"
     CDS             complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="IWO04_000676"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0212306.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8272..10551
                     /locus_tag="IWO04_000677"
     CDS             8272..10551
                     /locus_tag="IWO04_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0212307.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10644..11291)
                     /locus_tag="IWO04_000678"
     CDS             complement(10644..11291)
                     /locus_tag="IWO04_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212308.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11470..12189)
                     /locus_tag="IWO04_000679"
     CDS             complement(11470..12189)
                     /locus_tag="IWO04_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0212309.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12320..13471)
                     /locus_tag="IWO04_000680"
     CDS             complement(12320..13471)
                     /locus_tag="IWO04_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0212310.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13688..14428)
                     /locus_tag="IWO04_000681"
     CDS             complement(13688..14428)
                     /locus_tag="IWO04_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0212311.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="IWO04_000682"
     CDS             complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="IWO04_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0212312.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14812..15405)
                     /locus_tag="IWO04_000683"
     CDS             complement(14812..15405)
                     /locus_tag="IWO04_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0212313.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15389..16036)
                     /locus_tag="IWO04_000684"
     CDS             complement(15389..16036)
                     /locus_tag="IWO04_000684"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0212314.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="IWO04_000685"
     CDS             complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="IWO04_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0212315.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="IWO04_000686"
     CDS             complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="IWO04_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0212316.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17482..18009)
                     /locus_tag="IWO04_000687"
     CDS             complement(17482..18009)
                     /locus_tag="IWO04_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0212317.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18189..18974)
                     /locus_tag="IWO04_000688"
     CDS             complement(18189..18974)
                     /locus_tag="IWO04_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0212318.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18967..19836)
                     /locus_tag="IWO04_000689"
     CDS             complement(18967..19836)
                     /locus_tag="IWO04_000689"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0212319.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19837..21216)
                     /locus_tag="IWO04_000690"
     CDS             complement(19837..21216)
                     /locus_tag="IWO04_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0212320.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAKQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="IWO04_000691"
     CDS             complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="IWO04_000691"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0212321.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21668..22144)
                     /gene="accB"
                     /locus_tag="IWO04_000692"
     CDS             complement(21668..22144)
                     /gene="accB"
                     /locus_tag="IWO04_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0212322.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="IWO04_000693"
     CDS             complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="IWO04_000693"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0212323.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="IWO04_000694"
     CDS             complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="IWO04_000694"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0212324.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="IWO04_000695"
     CDS             complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="IWO04_000695"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0212325.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25174..25398)
                     /locus_tag="IWO04_000696"
     CDS             complement(25174..25398)
                     /locus_tag="IWO04_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0212326.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25451..26413)
                     /locus_tag="IWO04_000697"
     CDS             complement(25451..26413)
                     /locus_tag="IWO04_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0212327.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26423..26863)
                     /locus_tag="IWO04_000698"
     CDS             complement(26423..26863)
                     /locus_tag="IWO04_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0212328.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27169..28134
                     /locus_tag="IWO04_000699"
     CDS             27169..28134
                     /locus_tag="IWO04_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0212329.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28191..28421)
                     /locus_tag="IWO04_000700"
     CDS             complement(28191..28421)
                     /locus_tag="IWO04_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0212330.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28507..29394)
                     /locus_tag="IWO04_000701"
     CDS             complement(28507..29394)
                     /locus_tag="IWO04_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0212331.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29483..30706)
                     /locus_tag="IWO04_000702"
     CDS             complement(29483..30706)
                     /locus_tag="IWO04_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0212332.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30723..31802)
                     /locus_tag="IWO04_000703"
     CDS             complement(30723..31802)
                     /locus_tag="IWO04_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0212333.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31933..33660)
                     /gene="ptsP"
                     /locus_tag="IWO04_000704"
     CDS             complement(31933..33660)
                     /gene="ptsP"
                     /locus_tag="IWO04_000704"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0212334.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33660..33926)
                     /locus_tag="IWO04_000705"
     CDS             complement(33660..33926)
                     /locus_tag="IWO04_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0212335.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34405..36639
                     /locus_tag="IWO04_000706"
     CDS             34405..36639
                     /locus_tag="IWO04_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0212336.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36787..37107
                     /locus_tag="IWO04_000707"
     CDS             36787..37107
                     /locus_tag="IWO04_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0212337.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37312..37536)
                     /locus_tag="IWO04_000708"
     CDS             complement(37312..37536)
                     /locus_tag="IWO04_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0212338.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37734..39314)
                     /locus_tag="IWO04_000709"
     CDS             complement(37734..39314)
                     /locus_tag="IWO04_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0212339.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39715..41091)
                     /locus_tag="IWO04_000710"
     CDS             complement(39715..41091)
                     /locus_tag="IWO04_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0212340.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41219..42337)
                     /locus_tag="IWO04_000711"
     CDS             complement(41219..42337)
                     /locus_tag="IWO04_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0212341.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42479..42913
                     /locus_tag="IWO04_000712"
     CDS             42479..42913
                     /locus_tag="IWO04_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212342.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42991..43524)
                     /locus_tag="IWO04_000713"
     CDS             complement(42991..43524)
                     /locus_tag="IWO04_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0212343.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43618..44796)
                     /gene="mutY"
                     /locus_tag="IWO04_000714"
     CDS             complement(43618..44796)
                     /gene="mutY"
                     /locus_tag="IWO04_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0212344.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44789..45586)
                     /gene="recX"
                     /locus_tag="IWO04_000715"
     CDS             complement(44789..45586)
                     /gene="recX"
                     /locus_tag="IWO04_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0212345.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45693..47075
                     /gene="rlmD"
                     /locus_tag="IWO04_000716"
     CDS             45693..47075
                     /gene="rlmD"
                     /locus_tag="IWO04_000716"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0212346.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47249..47827)
                     /locus_tag="IWO04_000717"
     CDS             complement(47249..47827)
                     /locus_tag="IWO04_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0212347.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48149..48619
                     /locus_tag="IWO04_000718"
     CDS             48149..48619
                     /locus_tag="IWO04_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0212348.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48727..49953)
                     /locus_tag="IWO04_000719"
     CDS             complement(48727..49953)
                     /locus_tag="IWO04_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0212349.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50707..50907)
                     /locus_tag="IWO04_000720"
     CDS             complement(50707..50907)
                     /locus_tag="IWO04_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0212350.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51041..51649)
                     /locus_tag="IWO04_000721"
     CDS             complement(51041..51649)
                     /locus_tag="IWO04_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0212351.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51734..52204)
                     /locus_tag="IWO04_000722"
     CDS             complement(51734..52204)
                     /locus_tag="IWO04_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0212352.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52252..53166)
                     /locus_tag="IWO04_000723"
     CDS             complement(52252..53166)
                     /locus_tag="IWO04_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0212353.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53312..54115
                     /locus_tag="IWO04_000724"
     CDS             53312..54115
                     /locus_tag="IWO04_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0212354.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54293..55240
                     /locus_tag="IWO04_000725"
     CDS             54293..55240
                     /locus_tag="IWO04_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0212355.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        15908 a        11671 c         9445 g        18262 t
ORIGIN      
        1 tttttgtcac aacctttttt gccgagtaaa cggtgttcaa aagccagttt ctcggtatga
       61 agtcaaattt aagcaaaaat gtgagaagct attttcacaa aatttttctt catactcgaa
      121 actaagcggc tttttcacga cctctttttt attttgccat gaacatatca taatacgcgt
      181 ccattatttc tttagcaatc gtcaagttta tgtggtcttg ttcgtctaat aagttcggca
      241 agacaacact gatggcaatt tccggattgt ctgttggtcc gtatgccacg atattactat
      301 tgactgtcgc aatatctgga tgaccttccg caaccgtttc tgcagtccct gtttttgcgg
      361 aaaggtccat tttcgcactg gctaatggtt tggctgttgt gtaaggatcg gtaccatgga
      421 caacttggtg gaatccttca cggaggatcg ccatattttc tgcagaaata ttgaccgtat
      481 tttctactgt tgtgccaatt tctttcttca cttcacccaa gtttccattt tcgtcattgc
      541 catagatcgc tttgactagg tgcggctgta ctcgtttccc gccattagcg actgttgaag
      601 catattgagc taattggatt ggtgtatatg tgtcaaactg tccaaatgcc aaatcggtaa
      661 agttacctgg agcataccat tctttgccat tatccgcatt gaacttcttc atataatcaa
      721 cagaacgtga gatcccagga gattcattcg gcaaatcgat cccagtcgtt gtaccaagac
      781 caaactcttt gaaagagtct ctcaattgct gataagcttt agcttcttca tctaaactag
      841 gtagtcccat tccaggtgta tagtctagtc ctaacatctt taacgccact ttgatcatat
      901 acgtattaga agacaattcc gatgctttga ctgcatcaag agcaacttgt ccgcttcgat
      961 taaagacaga gcttttttct gaagcacctt gcagacgaat cggctcatca atcaaaactt
     1021 gatttccgct gatcgcgtta cttgcccagc ctgctgtcaa agtaccagcc ttgactacag
     1081 accctggatc aaaggcgcta gtaatcgtgc ctaaagcatt ctcggtgatt tctttggaac
     1141 cttgttcatg actgaaacca gtcattgcta gcacttctcc tgtctgagga ttcatagcca
     1201 ctgcataagc accaggcgaa tactgtgctt tgccgttgtt gatcaatgtt tgataattcc
     1261 ttttcaaaat ttcttctact tttgattgga attctgcatt catagataac attaaattag
     1321 aacctttttc cccagagaag atttcttttt gattcgaaac atttccttca ttatcaaggg
     1381 aaacttcata ttgggttttg gttccttgca ggacatcttc atattgtttt tccaaataac
     1441 tttgtcctac acggtcattc cgagaataac cttttgctag gtatttctct gcttcatctg
     1501 ctggaagtcc ttgcttttca gtcgtcacac tgccaagaat acttttcagt gaacttgctg
     1561 cattatattc tcgtgtccag tctgtgccag tcgaaactcc tggaagttca gaagcgcgtt
     1621 ctgccacaac agctaactct tgatcagtaa caccagagtt tttgatatag acagtgttta
     1681 attcatatgc actattcatc cgtttaaaga tggtagcaat ttttaattgc tcctcgttta
     1741 aattaatctc atcagctgtg actttattga ccatcgcact atattgttcg ctgttcccta
     1801 attgcttttc ttttgccgaa agtctttttg tggcttcttt caaatgtttg ggatcagcca
     1861 gccaaaaatc ttttttatct ctatctgtca gattttcatc taccggaaca tcgatcagcg
     1921 agttcagctt cgttgcgatt tcaagcaaat ctttagccgt aacttgtgtt cctctcgtat
     1981 atgtgatggc ctgattcgct ttattcgtaa ctaaagcttt tcctgtggag tcgtagatca
     2041 ttccgcgagg agtactttct tggaccgtaa tcgtagaagt agacttcact cgcgcctcca
     2101 tctcttcgcc gttcacgatc tgcaaataac ccaatctagc aatcaacgca acaaataacg
     2161 taaatataat gaaaaaaaga aaatttaagc gaaaaggaac atgggaccgg cgaaacgttt
     2221 tgttcttagg ttcttgtgca ccttttctta ttttttctaa ccaagaagta tttaatattt
     2281 ttttcataaa atctttcatc atttcctatc cttccgccta tttacattca tgcttcattg
     2341 tacccaaaaa cttcttaata aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa
     2401 tttcaagata aaatcaactt tttgtttcac agcaatcact taaagttaca tatccaacta
     2461 tttttttcat taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca
     2521 ctttgatgga tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa
     2581 aaaatagtaa ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt
     2641 tttcactttt tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat
     2701 cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg
     2761 tctagatgtt tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt
     2821 caacgcaatg atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc
     2881 acttctacct gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg
     2941 cgggagcaag acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt
     3001 ttgcagaata tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc
     3061 tgccgattcc aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt
     3121 ttcttttatc gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc
     3181 atttgcttct atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt
     3241 gattttgaag tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg
     3301 cgcactcaac ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat
     3361 attaccgata atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact
     3421 ttcaatacga ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag
     3481 tataccatat cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc
     3541 gcccaacaga atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg
     3601 aggaatacct gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat
     3661 ttacctatta gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga
     3721 tattcaaatc ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat
     3781 cttccaattc cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac
     3841 gtatcttata gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt
     3901 gcaaacgttc tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga
     3961 ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat
     4021 aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg
     4081 tattttcccc gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta
     4141 attgtatagt tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt
     4201 ttggtccgta aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca
     4261 tagcggcttt cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat
     4321 ctgtattgtt tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag
     4381 catatactgc caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac
     4441 gaacaaatgt atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag
     4501 cacctgattt ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac
     4561 gataagaatg gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa
     4621 gaacaagcat ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat
     4681 cccagtgtcc agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt
     4741 ggtaacctag actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg
     4801 ccccttttgg aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt
     4861 caagttcttt tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat
     4921 attctttcaa agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat
     4981 tgtttgagtt tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac
     5041 ggccagtgga tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag
     5101 cagtaatcac ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg
     5161 caaaaatttc taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt
     5221 tgacgatttt catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt
     5281 gtccattatc tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga
     5341 tatttggata gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta
     5401 agccaagtgc atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg
     5461 gacgagtcaa atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc
     5521 tttttgaaat gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg
     5581 caccatctgg aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat
     5641 cagatcatca agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac
     5701 gaaaagttcg tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg
     5761 gtccgaccgc ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg
     5821 gaaaagtttc agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc
     5881 tcatcaatca attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact
     5941 aaacttattt tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga
     6001 tgacatggat gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt
     6061 tcatcatatt ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg
     6121 gcataatgct ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg
     6181 tttgtcttgg gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc
     6241 aaaatagaga ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt
     6301 cccgtacttg caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt
     6361 ttcgctattc tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca
     6421 tctttcaaga tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt
     6481 gcaataccga tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc
     6541 gcaacaggtc tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag
     6601 ctacgggcat gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat
     6661 ccatccccgg tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc
     6721 tttgctgctt ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca
     6781 attgttacat agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca
     6841 cgtccaatct ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct
     6901 ttcagttgac taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct
     6961 aagatatggc tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt
     7021 attccattga ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt
     7081 ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca
     7141 acgatcccgt ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga
     7201 atcagtggac aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag
     7261 aaccaccaag aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga
     7321 tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc
     7381 atttgatact ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca
     7441 tttaaagacc ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct
     7501 gtcaaagaag attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca
     7561 agttcgccag gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc
     7621 tgcaattttc cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc
     7681 agttctttac ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg
     7741 atcaatatca tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta
     7801 atcgctaata catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac
     7861 atcaagaagg cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg
     7921 tgaaaaacaa attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt
     7981 ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat
     8041 ttccagctgc ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg
     8101 ccttaattga aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa
     8161 aaatattcag caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa
     8221 actatgctat acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag
     8281 aaacaactag aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat
     8341 atgctggata caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca
     8401 gctaatcaca agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat
     8461 cgccaacatg agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc
     8521 ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa
     8581 gaccgtgaaa cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa
     8641 acaatcgtta ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt
     8701 cccgcatttt atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa
     8761 tttaaaatcc aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat
     8821 gactttttgc tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct
     8881 acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt
     8941 gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg
     9001 tatcataatc gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg
     9061 ttttctgatt acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag
     9121 acattcaaaa attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa
     9181 caagaaagtg gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg
     9241 accttagtcg atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc
     9301 cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat
     9361 aaagaaacaa atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc
     9421 ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg
     9481 gcggaagaac agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa
     9541 aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga
     9601 atccatcgag ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt
     9661 ctacaaactg tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag
     9721 gaacatttga caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct
     9781 gtactattct ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc
     9841 tatatcttta ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc
     9901 ttgttcccaa aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat
     9961 gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa
    10021 ttaacgacaa gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg
    10081 aaagaacatc aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga
    10141 acaacagatg atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa
    10201 gcccggtatt ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat
    10261 gctcaactgc cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt
    10321 atgaagcgtt ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga
    10381 gttttcttgt gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt
    10441 tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt
    10501 cctttgttaa acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga
    10561 caactgctat cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa
    10621 gtaattcaat catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat
    10681 tttaatttga caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg
    10741 tcaaatacct tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc
    10801 cattcaatga attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa
    10861 atctctgata cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct
    10921 atcttctcat ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat
    10981 agtagaaaga aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa
    11041 cgacgaaaaa gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc
    11101 accatacact ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag
    11161 ccttcaacgc cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca
    11221 gctaactctt ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt
    11281 tttgcttcca tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac
    11341 ttatgctctc atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca
    11401 agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa
    11461 actggttgct tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc
    11521 ttccagtgca tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc
    11581 gttttgaggg atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa
    11641 tgcttcttgt atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc
    11701 ttccattaca tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc
    11761 gtcttctcct gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga
    11821 aacacccatg ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt
    11881 cacattgttc gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata
    11941 gcgcaattca gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat
    12001 aatatgtttt ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt
    12061 tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc
    12121 ataaatccgg ctattattag catctttagc agttttcttt gctacgatat ttgcccattt
    12181 acgtcccata ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg
    12241 ttccattata cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt
    12301 ctcattttta gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa
    12361 tattgcctcc caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct
    12421 ctttttttcc gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc
    12481 cagcatcggt aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt
    12541 gctttttgat attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc
    12601 tttttgtttt tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt
    12661 tcatgcgcga ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag
    12721 aagataacaa ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag
    12781 ttgaaagatc ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta
    12841 acgcatggcg aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat
    12901 agcctgcact tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt
    12961 cctttgcata acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg
    13021 gatacagact gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca
    13081 gttcttgaaa tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc
    13141 tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt
    13201 gcaagatttt tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg
    13261 gaagctccac tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat
    13321 cgatgatagc cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct
    13381 ctgctcctgt tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat
    13441 tgtactctac gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa
    13501 aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa
    13561 ctattctcgc aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat
    13621 atcgttcgga tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc
    13681 aaaaccgcta gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt
    13741 cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta
    13801 aataactttc caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac
    13861 cagacccttc ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc
    13921 ttcctggata actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt
    13981 ctggaaaaac ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt
    14041 cttctaacgc ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat
    14101 agcacagcga atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga
    14161 gcatattgcc ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa
    14221 tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg
    14281 caaccgctaa tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta
    14341 aatgacgttt cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt
    14401 aaatgaaagc aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta
    14461 gcggagcatc tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa
    14521 catgtacgat cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa
    14581 tacgttttac ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt
    14641 tgatttgacg ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat
    14701 ttcgaacgtc taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa
    14761 tttttaattg ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt
    14821 tgctacccag taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag
    14881 caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc
    14941 aatggctcgt gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac
    15001 ataaatgatt ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc
    15061 atgaagaatc tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag
    15121 accatgccag attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat
    15181 taccattttg aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat
    15241 actagctttt tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac
    15301 tccaagcaca tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt
    15361 ttctctagaa taagcagtat attcattact atagtccata ttcatacaac ccttcatttt
    15421 gaatataatc aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct
    15481 tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat
    15541 atggagtatc cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca
    15601 gctcatcgat tttataccat tttggcaaat attctaccat atccccaccg ataataaagt
    15661 aataatctgt atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt
    15721 tcccaccacg agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta
    15781 attccaacat gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg
    15841 gctgataaga aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag
    15901 ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct
    15961 ttttcttctc taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct
    16021 tagcttgcgt attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt
    16081 gatacttttc ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga
    16141 tttcgcaatc tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat
    16201 tttgaagtaa cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga
    16261 tcattgctga attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt
    16321 tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa
    16381 tcaatgcttt gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg
    16441 gcgctgttac agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag
    16501 aaaactcaaa acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta
    16561 ataatccacc tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa
    16621 tatttacatc atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa
    16681 atcttgcaag tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt
    16741 tgatttcttt taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt
    16801 gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct
    16861 cgattttatc caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg
    16921 ctgtattttt gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga
    16981 tatagacatc ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat
    17041 gagatttctt agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac
    17101 gttccttcat ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt
    17161 tgttcccaac taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg
    17221 aaccattgaa gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt
    17281 cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac atcttggatc tcattataat
    17341 ggcgtaatct gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag
    17401 ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc
    17461 cgatacattg taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt
    17521 tgctaataga taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact
    17581 atctgttgcg ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg
    17641 tatatcagtc ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg
    17701 tttctttgct atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag
    17761 atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac
    17821 agggattcct gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg
    17881 attattccat gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt
    17941 ctttaattgg gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt
    18001 tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac
    18061 cccgtattat actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa
    18121 aaagcatttc tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca
    18181 aaaaacaact aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct
    18241 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
    18301 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
    18361 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
    18421 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
    18481 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
    18541 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
    18601 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc
    18661 aaggtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
    18721 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
    18781 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
    18841 gcttcgtcat ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct
    18901 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
    18961 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
    19021 tttctgtttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
    19081 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
    19141 tccagcaaaa ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa
    19201 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
    19261 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
    19321 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
    19381 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
    19441 aaaattagga tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt
    19501 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
    19561 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
    19621 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
    19681 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
    19741 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
    19801 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
    19861 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
    19921 gactgcagga tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
    19981 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
    20041 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
    20101 tgcactgtct actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
    20161 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
    20221 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
    20281 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
    20341 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
    20401 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
    20461 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg
    20521 ttgtaaagaa caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat
    20581 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
    20641 aaatgccgct gctgcttctt gcttagctga agaaaaatgc tgtgggagct cttcttttga
    20701 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
    20761 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
    20821 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
    20881 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
    20941 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
    21001 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
    21061 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
    21121 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
    21181 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
    21241 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
    21301 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
    21361 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    21421 caagagaaag taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg
    21481 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    21541 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    21601 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct
    21661 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    21721 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    21781 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    21841 aagctggttt gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct
    21901 cgattccatc aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt
    21961 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    22021 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    22081 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    22141 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    22201 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    22261 gtcaatctca ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    22321 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    22381 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    22441 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    22501 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga
    22561 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat
    22621 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    22681 gcctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    22741 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    22801 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    22861 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    22921 aatacctttt gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat
    22981 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    23041 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    23101 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    23161 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    23221 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    23281 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    23341 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    23401 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    23461 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    23521 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    23581 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    23641 agattttgtt aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt
    23701 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    23761 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt
    23821 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    23881 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    23941 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    24001 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    24061 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    24121 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    24181 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    24241 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    24301 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    24361 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    24421 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    24481 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    24541 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    24601 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    24661 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    24721 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    24781 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    24841 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    24901 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    24961 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    25021 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    25081 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    25141 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    25201 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    25261 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    25321 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    25381 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    25441 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    25501 taaatattac tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca
    25561 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    25621 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    25681 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    25741 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    25801 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    25861 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    25921 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    25981 gcttagatag tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt
    26041 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    26101 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat
    26161 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    26221 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    26281 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    26341 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    26401 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    26461 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    26521 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    26581 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    26641 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    26701 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    26761 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    26821 cactaacaag ataatcattt actgttttta aatctggttc catgttccac cgtccactaa
    26881 ttagtttgat tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca
    26941 accttaaaag atcatttttt tactatatga gcttttttta agattttact catttaaaac
    27001 aactgataaa tcagcattta taatttcttt tattagaaaa atcatctatc tcacatattt
    27061 tgattatcaa agtattttct gtgaacagaa ctatagaatt ccctaaaact tgtccattat
    27121 ttcaaaattt gttatagtct ttttattcaa agcgtaaaag gagatcctat gttaaaagaa
    27181 aaaatgaaaa ctctccccat gctgccgggt gtctatctta tgaaagaccg agatggtaag
    27241 gtcatctatg ttggtaaagc aaaacggctc aaaaatagag taagcagcta ttttcatcag
    27301 aataaacagc attcaaaaaa agtattacgt atgattcatc atataacaga ttttgatttc
    27361 gtcgtagtag acacggaatt agatgcttta ttgctcgagt gtcaattgat ccaacattac
    27421 cgtccttttt ataaccgaca aatgaattat ttcagtaatt ataattatgt acatattacc
    27481 aataagggat ttgtattaac agatactcct acggctcgaa catacgggcc ttttcgtctc
    27541 tataaaaaaa tgccgtctat tttgcgtata atggaagaaa cttatcaaat gccttggttg
    27601 tctgaaatta gtttgttagc tttgcgagtc cagcttcctg acctacagga aatgactttt
    27661 gaacagaaaa aaaaggaact ccaagggctg tttcaaggcc gaaataaaaa gctactgact
    27721 tacttgaaaa aaagacaaca acatttcatc tatcagttga atttcgaaaa agccggtatg
    27781 cttcaaaaag atatcgaatt agttacttat tttattagac ggatccagga acaaaagcag
    27841 tttttaagaa caccatcgct caccttcagc atgccattag cagcagatga gagtcagaaa
    27901 aagcactacc tcgtttgtta tggtcaactt gcagaaacaa tgattacctc tggtgacgtt
    27961 tctcctgact tctattatga aaagaaagaa gctcatttat ctttgaaacg tcaattgtca
    28021 aaagaagaaa tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa
    28081 cagtttgaaa ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt
    28141 ctcggcttct tttaggcatc ttcaacttca aatgtcaatt atcctaaaat ttattggatt
    28201 aagtcataga tttccatcgc tacgatatcg atattatcaa attcgtattg ctgatgagta
    28261 tctgtttcag ttaattcaaa tgtatcggtc gctttatcat aagttactgt acacttctct
    28321 acgccttctt tttcaaaacg gcgaacttgc agttcattat ctgctgactc gaccatagcc
    28381 tccagtcgtt ttatgattgc gactaattgt gaatgtgtca tcggaggtcc tccttttctt
    28441 atttctgtta tattgtatca aaagcatagc ctacttgcat tgattttctc taaaaaaact
    28501 cgttttttat gatttttttt ctggtatttt tccttcaggc caccaaagtt tgatcaaagc
    28561 ttgggttccg tcattctcaa aacctttatc tgccaactct tcatatagtt tcaatgcttg
    28621 ggtagttgct ggcaatacaa gatccatttt ttctgcttct tctaatgcaa tctttaaatc
    28681 tttgataaaa tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga
    28741 ataattgctt aaagaccagt tagctgcact tccacctgaa agcgtctcaa tcactttctt
    28801 taaatcaagt ccgttttttt gtgcatagac aagcatttcc gtcattcctg tcatcgtacc
    28861 tgcaatcatg atctgattag ccatttttgt atgctgacct ttcccagcag aaccatgcaa
    28921 tgtaaatgtc gtaccaaatt ctttgaataa aggcagtact tgatcataaa cggcttcttc
    28981 tccaccgacc atgatcgtca gtgtaccatt ctttgctcct aagtcaccac cggataccgg
    29041 tgcatctaac gcatctgcgc ctttttcttt tgctgttttt gcaatttttt ctgccaggct
    29101 tggtgtactt gtagtcaaat caactaaaat atgtcctgag atatctgcag aaaaaatccc
    29161 cgactgtcca tagtagattt cttctacatc tttggggtaa cctaccattg taaagagaat
    29221 attactttgt tctgctaccg cttttggtgt atctgcccag atagctcctt ctgttactaa
    29281 aggatcagct tttgcttttg tccgattata gacagctact tcgtgtcctg cttctagtag
    29341 atggcgtgcg acagcacttc ccatgacgcc tgttccgata aaaccaagtt tcattttttc
    29401 gacctcctaa aaatcaaaaa tccttgcttc tatgctagta tacgctagaa acaaggattt
    29461 tttcataaaa aatgtttgaa ttttatcttt taaatgaatt taattttatt ttgattcgtt
    29521 caatcgagtc ttcttcttct tttcttcgca tctcttcttc atgataaaca accatgtctt
    29581 gataaaattc gatcatagct tcgccgaaat gtgtagatga aatatcataa agtttagctt
    29641 ctaaaacttt tggatcttta ggaacttcac tggaaatata atcaataagt gctggtgcaa
    29701 agtcttcgtc ggtttcaaaa gttttcccta atgaaggatg atcgaacaat cggttcaaat
    29761 aagcatttcc ttccacgaca caaggtgttc cggctgccat cgcttctgta taagtaagcc
    29821 cttgtgtttc tgaagtagaa gcactgacaa aataatctgc cgctttataa taaagtgcta
    29881 catcattgtt gtccacttct cctgtgaact gcacaaattc ttctaactgc agttctgaga
    29941 tcaattcctc taactcttct acataaggtc cttttcctac gataactaat ctggcgttag
    30001 gataagcgga gatgacctga ggaaacccat taatcaaggc ttggatattt ttctcataag
    30061 aaatcctgct taaagaaaga atcatcaaat ggttttcctg aagacctagt gactccctta
    30121 atttgctgat atcctgctga gtgatatcag aacgtttgaa tttttcaatg tcgataccag
    30181 taggaatcac gcgaagcggt gctattacac catagctgcg taatgtatca ataacacgtt
    30241 cacttggaca aactacacct gtcgtatgat ttgcaaataa tctagacaag tattttacat
    30301 gggattgcct gatcactttt cctttaccga tataatgaag gtagtcttca tacatcgtat
    30361 ggtaagtatg gatcagaggt attttcaact tcttagctac catcttccca agtatccctg
    30421 ctccgaattc tgtatgggta tgaataagat ctagttctag ttctttggca accaaataag
    30481 cataccacat gcctctaaca acaactcttc gatccttgaa agaaataaat ggtacacttg
    30541 gcattcgaat cacatctttt tccaacttat ccgcattagg atcggtcgtt gtaaaaatat
    30601 agacttcatg cccatgtctt tctaattctt gttttaatgt tttgatcgat gtagcaactc
    30661 cgctgacttg cggaaagtag gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca
    30721 aactagtcct tgacctgatt tacttttttt aatttgctag agactttttc attcagtaca
    30781 ctttcgtata catttttaag ttcttcaccg attttattga tacttcgctc ttttgctgtt
    30841 tgataaccgg cttgtgtcgt actcggtagt tcttgattgg taatctgttg gatcagtcgg
    30901 ataaattctt cgtttgtctt ccccatatag cagttcttgc tatcttctaa ccagccatta
    30961 tacactggaa tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg
    31021 atcccttctg tctcttcata agaagggaaa aagaatagat ctgcacctga atacgcacct
    31081 tctatgatct ctccacgaat gtaaccagga aacaggacat tttcaggatg atcttctttt
    31141 acaattttcc ggatgttccg aggaatcgag tacatgggaa catgaccaaa ccaaataaat
    31201 gtgtattcgg gcatcttctt agcaatttct acgaaatcaa tgattccttt acgttcgaaa
    31261 aacaaaccga cacaaatgat tactttttga tccggagaca gtttgaaata ttctctgaac
    31321 ttttgttctt tcattggatc agggtgatat ttctctaagt cgatcccatt ggaaatagct
    31381 tgaatcggca cacggatacc atagctttcc aacaactgtt ttgaataagg tgttggcgtg
    31441 atcagatagt ccgctttttg gtacaatcct accagatatt tttttacgat aggagaaagc
    31501 tggttcgatc cgataaagga atttcggaaa tcttcctctg tagaatgcgc atgatagaca
    31561 actttttttc cttctcttct ggctttattg accatattat gactattgat tccgtatgta
    31621 ttgatgtgca ggatgtcata atcttctttt tcatctagtg tataagagat accgacttcc
    31681 gtcagagcac gtttttgatg atcaagcgca cgcccgattc cagattttgc aagcatcttt
    31741 tcactttcaa aatataataa taccttcacc ttacgtcccc cttcacttat atagtataac
    31801 atagcctaca aactgtcact caaacttaag ccctattttt gtctattctt actgacaaaa
    31861 acgttttctt aaaaaagaac aaaaaaactg gcgtagctct tttacaagcc acaccagctt
    31921 ttgatacgtt gtttatttta catattcatg aacaagttca acgacttctt ccatcgtatc
    31981 gcattctttc aatgcacgat cagcaagttc agccattttc gctgtgtcca agcgtttcat
    32041 taaactacgc gttttaagaa tagatgtcgc actcattgag aactcatcta gacccatacc
    32101 tactagcaat ggaacagctg tttgatctcc agccatttct ccacacatac ctgcccattt
    32161 tccttcagcg tgtgctgcat caattacatt tttgatcaaa cgtaaaattg atgggttata
    32221 tggttggtac aagtaagaaa cgcgttcgtt catacggtct gctgccattg tgtattgaat
    32281 caaatcgttc gtacctacag agaagaaatc tacttctttt gcaaacttgt ctgctaagac
    32341 tgctgctgct ggaatctcga tcatgatgcc gacttggatc gtatcagata cttccttgcc
    32401 ttcacttatc aatttttgtt tttcttcttc aaagatcgct tttgctgcgc ggaattcttt
    32461 gagtgtagct accataggga acatgatacg taaattacca tgaacagatg cacgaagcaa
    32521 tgcacgcatt tgtgtacgga acatttcgtc gccttgttca gataaactga tacgcaatgc
    32581 acgatatcct aagaatggat tcatttcatg aggtaattgc agatatggta gttctttatc
    32641 tccaccgatg tccattgtac gaacaactac aggttttcct tccatacctt ctaatacagc
    32701 tttgtaagct tcatattggt catcttctgt tgggaagtct ggagaatcca tatacaggaa
    32761 ttcagtacgg tacagaccaa ctgcttctcc accattatta tggacaccaa ctagatcttt
    32821 tggtgttccg atgtttgcag ctaattcaaa atgcttgccg tcagctgtga cagtttctgc
    32881 attttttaat ttttcccatt ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa
    32941 ttcagctttt tgttcatctg taggatcgat gattacatcg ccttcgatcc cgttgacagc
    33001 caaaatatcg ccttctttga ctttagcagt aatttctttt gtcccaacga ttgctggaat
    33061 ttctaaagaa cgtgccatga tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa
    33121 cgcttttaca aaattgcgat ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac
    33181 aacgaccact tcttcgttga tcattgatgg atttggtaaa gtaacgccta ataagtgcgc
    33241 taaaatacgt ttagcaacgt cacgaatatc tgcagcacgc tcttgcatat aagcattatc
    33301 ttccattgct tcgaacatac cgatatacat atccgttact tcttttagcg ctgattcagc
    33361 atttacgctg ttgtctttaa tattttgctt gatttgacct accatttctg gatctgaaag
    33421 aaccatcaaa tgggcatcaa atacttgcgc ttctgcttca cctaagcttt gcgctgcttt
    33481 ttcacgaatt tgctgcaatt cttcagtaga tttagctaat gcagcatcca aacgagcttc
    33541 ttcagccgca gtatcttcta ctgttgcctt actgaatgtt aaatccggtt gaactagcag
    33601 gtaagctttt gcaacggcta caccgtcact agcggcaatc ccttttagca tttcaaccat
    33661 tattcagata agccttcttt cttcattgtt tcaacgattg ctgccattgc atcagcttcg
    33721 tcagcacctt ctgcagtaat agtaacatca gaaccttgac ctacacctaa agacataacg
    33781 cccatgattg atttcaagtt tactgattta cctttatact ctaagtttac atcagagtta
    33841 aatttgcttg ctgtttgtac taatagtgta gctggacgag cgtggatccc agtttctgct
    33901 actacgtgaa attctttctt ttccatatta atcggtctcc tttaagtgag tgttgttttt
    33961 atgtgagggt tttctttttt agtaattcag agaatcctaa caaatgccac cctttcatct
    34021 gtaaagatta acattttttt tcgttagata caacagtttt cattcaattt cagataaaag
    34081 tattcaaatt attccggatt ctttttcatc cttttcttca cattttataa caaacgtgtt
    34141 tatttccttt ttcttgtcgc tttctcttct cctgacgttt atcattcttt cctttgaccc
    34201 tctttgtttt catatgaaac gaattacttg cttccctttt tatttttagt ataataagag
    34261 tcaaaggtca ggaatggtca atagagattt caccttttct acctcataaa tctcttaaaa
    34321 aactaggggt tattctttta cctcatgaaa aacgagatgt aaaatatgga tgtacccatt
    34381 tttgtgaaag gacgtgaatg attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg
    34441 attcacttgt atgccactgt caatggtcag cggacacaac tcgattactg tcagagctgt
    34501 tatcaaaaaa tcaaaaatca acaaaatgga ggtctgacta atatggccca aaatgatcca
    34561 tttggatttg gcagtttgga cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa
    34621 ggaaattatg agcaaacacc accaacacaa tttggcggaa acaacaactt caatggtggt
    34681 cagccacctc aaggcaatac agaaggttta ttgggagaat acggaatcaa tatcacagaa
    34741 caagcacgac aaggtgatat cgatcctgtg atcggacgcg atgacgaaat caaacgagtc
    34801 attgaaattt taaatcgccg aaccaagaac aatcctgtct tgattggaga accaggtgtt
    34861 ggtaaaacag cagttgttga aggtttagca caaaaaatcg tagatggtga tgtaccgcaa
    34921 aaattaatgg acaaagaagt cattcgttta gatgtcgtat ctttagtcca aggtactggt
    34981 atacgaggac aatttgaaga acgtatgcaa aaattgattg aagagatccg tcaagcagaa
    35041 aatgtgatcc tattcataga tgaagttcac gaaatcgtcg gtgctggttc tgctggtgat
    35101 ggaaatatgg atgctggtaa tattttaaaa ccagctttag ctcgaggaga attgcaaatg
    35161 gttggtgcta ctaccttaaa tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt
    35221 cgtatgcaac ctgtacgggt agatgaacca actgtcgaag aaacgatcag tattttgaaa
    35281 ggtttgcaaa aacgttatga agactatcat catgtaaaat atactgatga ggccatcgaa
    35341 gcagccgcaa ctttatccaa tcgttacgtc caagatcgct tcttgccgga taaagccatc
    35401 gatttgctag atgaaacagg ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa
    35461 acaatcgaga aaaaactgca agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa
    35521 gatttcgaaa aagcagctta ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa
    35581 cgtcagctca ctgaagagga aacacctgtg attaccgaaa aagatatgga aaaaatcgtt
    35641 gaacaacgga caggtattcc agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa
    35701 aacttagcag atgacttgaa agctcatgtc atcggacaag ataacgctgt agatcgagta
    35761 gcgaaagcga ttcgccgaaa ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc
    35821 ttcttatttg ttggaccaac tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac
    35881 gaactattcg gttcacaaga ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa
    35941 catagtgtct ctaaattgat tggttctcct ccaggttatg tcggatacga agaagctggt
    36001 caattaacag aaaaagtacg gagaaatcct tacagtcttg tcttgttaga tgaagtagaa
    36061 aaagctcatc cagatgtttt gcatatgttc ttgcaaatct tagatgatgg tcgattgaca
    36121 gatgcacaag gaagaacggt cagctttaaa gatacgatca tcattatgac aagtaatgcc
    36181 ggaactggta aagtagaagc aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt
    36241 tctgtattga accaattaaa caactacttt acaccagaat ttctgaaccg ttttgacggg
    36301 atcatcgaat tcagtgcatt atcaaaagaa aacttgatga caatcgtcac actaatgcta
    36361 gacgatgtca accaaatgct ggcagcacag caacttcaca tcgaagttcc tacgaatgtc
    36421 aaagaaaaat tagttgactt aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc
    36481 acgatccaag aacaaatcga agatggtatc gctgaatttt atcttgatca cccttctatc
    36541 catgagttga aagcaaaact ggataaagat ggaaaaatca tcgttacttc taaaccagaa
    36601 cgtttggcta aagaatcagc cgaagaaacg gctgaataaa tccagtttta tagcgtgaga
    36661 cggtatttct ttgagaaatg ccgtctcatt cttttttcag aaaagtcaaa atactgttca
    36721 ttttataaaa aagaaattat aataagagaa acaagcaatt aaaaatacat aaaagaggtg
    36781 agcgccatga aattggtaaa tgtaacaaac agccattccc gtcttgtatt aaaccagctt
    36841 gaaaacactg atgcccatct agtcaaggtc tacacagccg gaaataccac tattgtctac
    36901 actgaagccc ctgaacataa tgaaattctg ctgatcaatg acaaacgtaa aatccagccg
    36961 aaagaaatcg aagatgctaa aaattatttc ttagataaaa tcaaagacgc cgtttaccac
    37021 gaggatgaaa tcaaagtaat cgaattggca ggccttgttg aaatctcgat tccaaaacat
    37081 gaattaggcg ctacattagc cgtgtaaaca aattttgaac ataataatta aagagcgtgg
    37141 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cgaaagcaga
    37201 agcaactcct tcggaaataa gctgaaattc acgaaaattg cttttcaaat ttttgtgaat
    37261 tccagcttat ttcttggagt taaacgcttc tgtctcagcc tctttttctt tctagctttt
    37321 tgatttttct cttttctttt gtacttttct cttttcattt tcagttctac gcgtgtcttt
    37381 atttttaact ttttcctctt tatggatgat tgatcgtact ttttgtgcaa tttgctcgtt
    37441 tttgtcccag tcttttacat ctaataatcg acataatcgt aacatctctt cttttttctg
    37501 tttttttctt tcacgtcttt gccattctgt ttccatgtgt cacactctcc tttctttaat
    37561 catatgtacg caaaataatt tttatatttt tttgattaga catttctatg attttaatat
    37621 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
    37681 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
    37741 aaacttttta attttacatt gggatattta tccgcaaacc agcgttctgc aaattggttc
    37801 tcgaataaga acaacggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
    37861 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
    37921 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
    37981 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
    38041 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
    38101 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
    38161 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
    38221 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
    38281 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
    38341 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
    38401 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
    38461 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
    38521 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
    38581 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
    38641 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
    38701 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
    38761 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
    38821 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
    38881 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
    38941 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
    39001 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
    39061 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
    39121 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
    39181 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
    39241 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
    39301 tttggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
    39361 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
    39421 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
    39481 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
    39541 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcagc
    39601 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
    39661 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
    39721 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
    39781 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
    39841 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
    39901 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
    39961 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
    40021 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
    40081 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
    40141 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
    40201 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
    40261 gccgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
    40321 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
    40381 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
    40441 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
    40501 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
    40561 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
    40621 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
    40681 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
    40741 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
    40801 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
    40861 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
    40921 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
    40981 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
    41041 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
    41101 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
    41161 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
    41221 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
    41281 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
    41341 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
    41401 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
    41461 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
    41521 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
    41581 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
    41641 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
    41701 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
    41761 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
    41821 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
    41881 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
    41941 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
    42001 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
    42061 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
    42121 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
    42181 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
    42241 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
    42301 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
    42361 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
    42421 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
    42481 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
    42541 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
    42601 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
    42661 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
    42721 cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg ctaagaatca
    42781 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
    42841 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
    42901 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
    42961 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
    43021 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
    43081 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat tcatcatttt
    43141 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
    43201 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
    43261 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
    43321 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
    43381 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
    43441 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
    43501 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
    43561 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
    43621 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
    43681 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
    43741 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
    43801 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
    43861 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
    43921 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
    43981 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
    44041 tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct ttgctttttt
    44101 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
    44161 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
    44221 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
    44281 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
    44341 tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac ttgcaatcgc
    44401 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
    44461 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
    44521 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
    44581 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
    44641 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
    44701 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
    44761 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
    44821 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
    44881 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
    44941 aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc gcttgttgga
    45001 tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc aataattctt
    45061 tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
    45121 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
    45181 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
    45241 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
    45301 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
    45361 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
    45421 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
    45481 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
    45541 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
    45601 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
    45661 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
    45721 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
    45781 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
    45841 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
    45901 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
    45961 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
    46021 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
    46081 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
    46141 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
    46201 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
    46261 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
    46321 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
    46381 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
    46441 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
    46501 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
    46561 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
    46621 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
    46681 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
    46741 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
    46801 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
    46861 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
    46921 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
    46981 aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact gcacgttgtg
    47041 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
    47101 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatact
    47161 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
    47221 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
    47281 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
    47341 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
    47401 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
    47461 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
    47521 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    47581 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    47641 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    47701 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    47761 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    47821 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    47881 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    47941 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat caaatcatat
    48001 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatt
    48061 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    48121 actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    48181 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    48241 aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    48301 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    48361 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    48421 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    48481 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc atcgcctggc
    48541 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    48601 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    48661 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    48721 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    48781 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    48841 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    48901 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    48961 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    49021 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    49081 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    49141 ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat caagcatgtc
    49201 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    49261 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    49321 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    49381 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    49441 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    49501 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    49561 catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc cataacctac
    49621 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    49681 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    49741 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    49801 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    49861 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga atacccctaa
    49921 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    49981 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    50041 aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt cttaactatc
    50101 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    50161 ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    50221 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    50281 atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    50341 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgattgcat
    50401 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    50461 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    50521 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    50581 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    50641 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    50701 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    50761 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    50821 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    50881 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
    50941 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    51001 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    51061 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    51121 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    51181 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    51241 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    51301 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    51361 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    51421 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    51481 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
    51541 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    51601 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    51661 tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga aggaaattct
    51721 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    51781 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca ataatttgag
    51841 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    51901 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    51961 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    52021 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    52081 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    52141 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    52201 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    52261 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    52321 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    52381 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    52441 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
    52501 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    52561 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    52621 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    52681 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    52741 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    52801 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    52861 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    52921 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    52981 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    53041 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    53101 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    53161 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    53221 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    53281 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    53341 ctttatacga ttcaaagacc taaaggagac gagaaatgtc ccattttgga aagccattgt
    53401 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    53461 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    53521 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    53581 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    53641 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    53701 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    53761 tttaaaaaac ttaacaggta aggagctccg ttctttttta agtaaagcat ctataccact
    53821 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    53881 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    53941 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    54001 aacaatcttg atggtctttc ttccttcttt tgacacaagc gccttcattg cttttgcagg
    54061 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    54121 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    54181 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    54241 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    54301 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    54361 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    54421 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    54481 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    54541 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    54601 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaagcacaga gtgacatgaa
    54661 atggttaggt tcatcggtcc attaccaatc tatccaagga aaaaaaatac taatcattgg
    54721 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    54781 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    54841 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    54901 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    54961 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    55021 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    55081 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    55141 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    55201 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    55261 aaaagaacaa agagaaggtg acaaaa
//
LOCUS       DACECB010000011        55155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECB010000011 DACECB010000000
VERSION     DACECB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.011"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            17..154
                     /locus_tag="IWO04_000726"
     CDS             17..154
                     /locus_tag="IWO04_000726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212356.1"
                     /translation="MAEKSKKILVLEKIGIIDGYQHPNEKELLRTMTVFQQIVSEIRG
                     N"
     gene            156..1415
                     /locus_tag="IWO04_000727"
     CDS             156..1415
                     /locus_tag="IWO04_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0212357.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1421..2002
                     /locus_tag="IWO04_000728"
     CDS             1421..2002
                     /locus_tag="IWO04_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0212358.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2084..2959)
                     /locus_tag="IWO04_000729"
     CDS             complement(2084..2959)
                     /locus_tag="IWO04_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0212359.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3107..3439
                     /locus_tag="IWO04_000730"
     CDS             3107..3439
                     /locus_tag="IWO04_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0212360.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3551..4759
                     /locus_tag="IWO04_000731"
     CDS             3551..4759
                     /locus_tag="IWO04_000731"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0212361.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4913..5368
                     /locus_tag="IWO04_000732"
     CDS             4913..5368
                     /locus_tag="IWO04_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212362.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5730..6005
                     /locus_tag="IWO04_000733"
     CDS             5730..6005
                     /locus_tag="IWO04_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212363.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6018..8096
                     /locus_tag="IWO04_000734"
     CDS             6018..8096
                     /locus_tag="IWO04_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0212364.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8256..10142
                     /locus_tag="IWO04_000735"
     CDS             8256..10142
                     /locus_tag="IWO04_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0212365.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10154..11101
                     /locus_tag="IWO04_000736"
     CDS             10154..11101
                     /locus_tag="IWO04_000736"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0212366.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11121..11648
                     /locus_tag="IWO04_000737"
     CDS             11121..11648
                     /locus_tag="IWO04_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0212367.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11711..12364)
                     /locus_tag="IWO04_000738"
     CDS             complement(11711..12364)
                     /locus_tag="IWO04_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0212368.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12496..13338
                     /locus_tag="IWO04_000739"
     CDS             12496..13338
                     /locus_tag="IWO04_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0212369.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13496..14392
                     /locus_tag="IWO04_000740"
     CDS             13496..14392
                     /locus_tag="IWO04_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0212370.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14395..15108
                     /locus_tag="IWO04_000741"
     CDS             14395..15108
                     /locus_tag="IWO04_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0212371.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15128..15646
                     /gene="msrA"
                     /locus_tag="IWO04_000742"
     CDS             15128..15646
                     /gene="msrA"
                     /locus_tag="IWO04_000742"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0212372.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15643..15870
                     /locus_tag="IWO04_000743"
     CDS             15643..15870
                     /locus_tag="IWO04_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0212373.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16022..16573
                     /gene="lepB"
                     /locus_tag="IWO04_000744"
     CDS             16022..16573
                     /gene="lepB"
                     /locus_tag="IWO04_000744"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0212374.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16656..17198)
                     /locus_tag="IWO04_000745"
     CDS             complement(16656..17198)
                     /locus_tag="IWO04_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0212375.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(17435..17833)
                     /locus_tag="IWO04_000746"
     CDS             complement(17435..17833)
                     /locus_tag="IWO04_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0212376.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            18093..18953
                     /gene="ylqF"
                     /locus_tag="IWO04_000747"
     CDS             18093..18953
                     /gene="ylqF"
                     /locus_tag="IWO04_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0212377.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            18946..19713
                     /locus_tag="IWO04_000748"
     CDS             18946..19713
                     /locus_tag="IWO04_000748"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0212378.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            19769..20626
                     /gene="dprA"
                     /locus_tag="IWO04_000749"
     CDS             19769..20626
                     /gene="dprA"
                     /locus_tag="IWO04_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0212379.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            20748..22826
                     /gene="topA"
                     /locus_tag="IWO04_000750"
     CDS             20748..22826
                     /gene="topA"
                     /locus_tag="IWO04_000750"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0212380.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            22795..24171
                     /gene="trmFO"
                     /locus_tag="IWO04_000751"
     CDS             22795..24171
                     /gene="trmFO"
                     /locus_tag="IWO04_000751"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0212381.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            24369..25274
                     /gene="xerC"
                     /locus_tag="IWO04_000752"
     CDS             24369..25274
                     /gene="xerC"
                     /locus_tag="IWO04_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0212382.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            25311..25859
                     /gene="hslV"
                     /locus_tag="IWO04_000753"
     CDS             25311..25859
                     /gene="hslV"
                     /locus_tag="IWO04_000753"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0212383.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            25873..27273
                     /gene="hslU"
                     /locus_tag="IWO04_000754"
     CDS             25873..27273
                     /gene="hslU"
                     /locus_tag="IWO04_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0212384.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            27291..28082
                     /gene="codY"
                     /locus_tag="IWO04_000755"
     CDS             27291..28082
                     /gene="codY"
                     /locus_tag="IWO04_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0212385.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            28164..29039
                     /locus_tag="IWO04_000756"
     CDS             28164..29039
                     /locus_tag="IWO04_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0212386.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(29171..29809)
                     /gene="plsY"
                     /locus_tag="IWO04_000757"
     CDS             complement(29171..29809)
                     /gene="plsY"
                     /locus_tag="IWO04_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0212387.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            29936..30370
                     /locus_tag="IWO04_000758"
     CDS             29936..30370
                     /locus_tag="IWO04_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0212388.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            30536..32581
                     /gene="parE"
                     /locus_tag="IWO04_000759"
     CDS             30536..32581
                     /gene="parE"
                     /locus_tag="IWO04_000759"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0212389.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            32593..35043
                     /gene="parC"
                     /locus_tag="IWO04_000760"
     CDS             32593..35043
                     /gene="parC"
                     /locus_tag="IWO04_000760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0212390.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            35320..37551
                     /gene="pflB"
                     /locus_tag="IWO04_000761"
     CDS             35320..37551
                     /gene="pflB"
                     /locus_tag="IWO04_000761"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0212391.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            37795..38556
                     /gene="pflA"
                     /locus_tag="IWO04_000762"
     CDS             37795..38556
                     /gene="pflA"
                     /locus_tag="IWO04_000762"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0212392.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            38641..39567
                     /locus_tag="IWO04_000763"
     CDS             38641..39567
                     /locus_tag="IWO04_000763"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0212393.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            39749..40642
                     /locus_tag="IWO04_000764"
     CDS             39749..40642
                     /locus_tag="IWO04_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0212394.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            40776..40901
                     /locus_tag="IWO04_000765"
     CDS             40776..40901
                     /locus_tag="IWO04_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212395.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(41035..41667)
                     /locus_tag="IWO04_000766"
     CDS             complement(41035..41667)
                     /locus_tag="IWO04_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212396.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(41815..42357)
                     /locus_tag="IWO04_000767"
     CDS             complement(41815..42357)
                     /locus_tag="IWO04_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0212397.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            42492..42881
                     /locus_tag="IWO04_000768"
     CDS             42492..42881
                     /locus_tag="IWO04_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212398.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            42885..43649
                     /locus_tag="IWO04_000769"
     CDS             42885..43649
                     /locus_tag="IWO04_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0212399.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            43778..44725
                     /locus_tag="IWO04_000770"
     CDS             43778..44725
                     /locus_tag="IWO04_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0212400.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            44855..45595
                     /locus_tag="IWO04_000771"
     CDS             44855..45595
                     /locus_tag="IWO04_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212401.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(45886..46554)
                     /locus_tag="IWO04_000772"
     CDS             complement(45886..46554)
                     /locus_tag="IWO04_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0212402.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            46688..46975
                     /locus_tag="IWO04_000773"
     CDS             46688..46975
                     /locus_tag="IWO04_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212403.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            47391..48929
                     /locus_tag="IWO04_000774"
     CDS             47391..48929
                     /locus_tag="IWO04_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0212404.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            48926..49891
                     /locus_tag="IWO04_000775"
     CDS             48926..49891
                     /locus_tag="IWO04_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212405.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            49878..51200
                     /locus_tag="IWO04_000776"
     CDS             49878..51200
                     /locus_tag="IWO04_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0212406.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      51263..51367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            51441..52184
                     /locus_tag="IWO04_000777"
     CDS             51441..52184
                     /locus_tag="IWO04_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212407.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            52199..53191
                     /locus_tag="IWO04_000778"
     CDS             52199..53191
                     /locus_tag="IWO04_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212408.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            53203..53940
                     /locus_tag="IWO04_000779"
     CDS             53203..53940
                     /locus_tag="IWO04_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212409.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            53930..54652
                     /locus_tag="IWO04_000780"
     CDS             53930..54652
                     /locus_tag="IWO04_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0212410.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(54736..>55155)
                     /locus_tag="IWO04_000781"
     CDS             complement(54736..>55155)
                     /locus_tag="IWO04_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0212411.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        19065 a         9426 c        11554 g        15110 t
ORIGIN      
        1 ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata cttgttttag aaaaaatagg
       61 tataatagac ggataccaac atccaaacga aaaagaatta ttgagaacaa tgacggtatt
      121 tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa tacaaatagc gaaaaaatgt
      181 tgcaagcttt atcagatgaa gacttgtcac aagcacaaat ctttttagaa aaagcattaa
      241 aagaagaccc agcagatata ttagctgaac taggcgaaga attgctggct attggctttt
      301 tgatcgaagc aaaacagatt tttgaacaac tgatcaacca atacccagaa aatgacggac
      361 tgaacatccc gttagcagaa attgcaatcg aagataatga gatcgactta gcttttgaat
      421 acctggaaaa agtggataag caaagtgatt actatccgca aagcttattg gcgatagctg
      481 atttgtacca agttatcgga atccctgaag tgagtgaagc aaaattgaaa gaagcagctg
      541 ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc tgaattgtat ttttcagttg
      601 atcgtttcaa agaggcagca gtggtttatg aaacattgtt ggcaagcggg ataaatgaaa
      661 tttcaggaat ctcgatgcag gaacgtttag gacaatcctt aagtatgcaa ggagaattcg
      721 aagcagctat cgtaccttta gaagaagcac tgaaagaaga aaggacagat gatcgactgt
      781 tccagctagc ttttacgaat cttcaattga aagaaaatca gcaggcaatc aactatctgg
      841 aagaacttcg agaagttaat ccgcattatc aatcgttata tctatatcta ggacaagctc
      901 ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt agaagaagga atcaaagaaa
      961 atccttacca agtcgaactt tatcacctgg cctcggaaaa tgctttccgt ctgcatgata
     1021 gacagcgagc agaagaactt ttactgaaag ctttagaagt aggagacaaa caagatgaaa
     1081 cattacttac tttaagtaat ctttacttgg atgatgaaag atatgaagat gtgatacaag
     1141 tgatcaatca gatggaagaa actgctaatc cttatgcaga atggaaccta gcccatgctt
     1201 ataatgaatt agaagacttt accgttgcag ctgtacacta tgaacaggct tatcatgaac
     1261 tatcacatga accagatttc ttgaaagagt atgccttttt cttaagagaa gaagggcagc
     1321 tgaaaagagc gcaagaatta ttgacttatt atttaacact tgaaccagga gatatggagg
     1381 cactgtcatt attagatgac ctaacagaga ggtgagaaaa atgctaattg atgtacggga
     1441 aaagaagaat tttttaacgt ggatggtcaa tcatgtatct tttagccgaa gagaagtttt
     1501 ttggatcttg aattatcttg ccaatcatga agcgatttta agcaatgtcc actttgttga
     1561 aggggcgaat tttgcggatc gaggactgca gatcagtgat ctatctgttg aaggggaacc
     1621 catgaaattg tttttgcagg gaaaagaatt tacggacaca gaccagattt ttcatgaaat
     1681 ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata ttcgaaaatg cttggcagac
     1741 aagagaatat ctatctatct tagaagacaa tccattttct tcttgggatt cttcagtcag
     1801 tgaagaagat actgaattaa ttgaacgata ttttcaagaa aaagaagaag aggcaaaact
     1861 aaaaatgctt tatgctcaaa tcgatcaagc actagaagat ggagataggg atgctttttt
     1921 gaaattatct gatgaagtta accgaaagat ccttttgcag acacaaaaga aaagtaaccg
     1981 ggtaagtgaa cataacaatt agttaaaaat agtatagtac taaaaaagac aaaggtcagc
     2041 tggatgtgga atctttcctc atagcctatc tttgtctttt ttcttatagt tttttcagta
     2101 atcttgtctg tggcttcaaa taagtaaagc cttgcccttc cacttcatgc acattgatga
     2161 ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct cttagcttca gttaattcac
     2221 tagagctaac gaccatatag atgatttttt ttgaattacc agagaatgcg ccttctccag
     2281 aaaggaaagt cacacctctt tgcagaccgg tcatcagtag tggtgcaatc tcttcgcttt
     2341 tgttggagac gacaagtaga ccttttgcag aatatccgcc atctaagatc gaatcaatca
     2401 cacgactgaa aacatacgaa acaatcaacg tatacatcat acgtttcaag tcgatatagg
     2461 ttaatgaagc aagtaagacg atgatatcaa atgccaatag ggaacgcccc atagtgatgc
     2521 cgtaattttg ttctaaaatc cgagcgataa tatcactgcc gcctgttgtt ccacccatgc
     2581 ggtagacgat cccgcttcct acacctgcaa ctaaacccgc aagtagagaa acgatcagta
     2641 gatcatgctg taaattgatt tctagtggaa ccctttgcca gatccataaa aagaatgaca
     2701 aagaaagtgt acctagaaca gtataggcca aggaacgttt accgaggatc tttcctccta
     2761 gtaaaatcaa tgggatgttg attaataaag tcgtataagc tggatcaata ccaaataaag
     2821 ctcttaagat caaggtgatc cctgagactc ccccttctgc taaatgatta gcgatgttca
     2881 agtaaacaag accaaatgca taaatacttg ttcctataat gataaaaaga acttctttta
     2941 ctgtttttcg tttgaccata tgaattccag ctttcaatta tgtattttca cagaatttcc
     3001 atttagaaat tccttcctaa atgtagcatg gaaattcgat tttctcaatt gctcttatct
     3061 aggatttgcg ttaaagtttt ggtaagatag ttttggaggg aaaaagatgg ataaaaaacg
     3121 ttctttatac agtatgcagc aggaagtgga tgaatatatc cagcaattca aggtggggta
     3181 tttctctcca ttagctcaga tggctcgatt aactgaagaa gtaggtgaat tggcaagaga
     3241 agtcaaccac acttatggag aaaaaagcaa aaaggcttct gaaccagtta actcggtagc
     3301 agaagaactt ggtgatgttt tattcgttac gatgatcatg gctaattcat tgaatatcga
     3361 cttaactgaa gtttttgaaa aaaacatgga aaaattcaat caaagagacc ataatcgctt
     3421 tgagcgtaag gacgagtaaa caagatgaag tggtcctggc cttgaagact ctgaaacgga
     3481 cttgtcaaac agtcaccatg caactgagtc tttatctcag gacaagaaac acgcaggaaa
     3541 ggttggaaaa atgaaattaa aacaattacc tctggaatac caaaaggcaa ttccagttct
     3601 gaaaaaatta gagaatgcag gatatgaagc ttattttgta ggtggaagtg ttcgtgatat
     3661 cttgctgaac cagccaatcc atgatgtgga tatcgcaaca agcgcatttc cagaagaaat
     3721 caaacagctt tttccaaaaa cgatagatat tgggatcgaa catggaactg tgttggtatt
     3781 agaaaatgat gaacaatatg agatcaccac atttcggacg gaatcaactt atcaggacta
     3841 ccgtagacca gatcatgtcg aattcgttcg ttcgttggca gaagatttga aaagaagaga
     3901 ttttacgatc aatgcgtttg ctttgaaaga agatggagaa atcatcgatt tgttcgatgg
     3961 tctgcaagat ctagaagaac agatcttgcg agctgtaggc aatcctcatg agcggttcca
     4021 tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc agtcaactag ggttcaaatt
     4081 agaggaagaa accttctacg ccatcaaaga aaaccacgag ctattaggta aaatatctgt
     4141 agaacgtata aatgtggaat tcatcaagat gctcttggga aatgaacggg ccgcgggaat
     4201 ccatgcattt attgaaacag agtgctacat ttgctgtccc ggtttgaaag aagaaggcga
     4261 agcactttta cgttttgctg aattaccatc cgtcccattg aaagaagaga gccaagcatg
     4321 ggcattattg atcagccaac tggatctaaa agaaaatgag attcgttcct ttttgaaatc
     4381 gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca cttgtccaag gactacgaca
     4441 acgagaaact ggagctttgg aaccattcga attgtacttc ttaggcaaaa aacatgcact
     4501 gcaagtagaa gaattgatga tctattttga cgaaacacct cagatggatg ccgtaggaga
     4561 atcctatgat cgtttaccta tcaagtcatt aagcgaatta gcagtagatg gtaaaatctt
     4621 gctagaagaa actggaaaaa aacctggtaa gtgggttagt gaagctttgc aattagctga
     4681 aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa caggttttgg attttttatc
     4741 gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc tcagctttgt tggctactta
     4801 aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg
     4861 tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa
     4921 agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg
     4981 tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag
     5041 tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga
     5101 cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc
     5161 attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct
     5221 tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac
     5281 aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga
     5341 agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa
     5401 gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt cagaataagg
     5461 gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag
     5521 gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact
     5581 tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac
     5641 tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga
     5701 tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac
     5761 tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt
     5821 tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc
     5881 gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag
     5941 tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa
     6001 aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca
     6061 ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg atccaaattt
     6121 tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat
     6181 tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt
     6241 cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga
     6301 cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct
     6361 gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg
     6421 gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg
     6481 atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt
     6541 cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct
     6601 ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag
     6661 atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag
     6721 aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg
     6781 gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga
     6841 caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc
     6901 aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta
     6961 tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg
     7021 tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat
     7081 ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca
     7141 ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag
     7201 tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa
     7261 gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa
     7321 taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt
     7381 atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag
     7441 tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc
     7501 gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg
     7561 aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta
     7621 ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc
     7681 agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc
     7741 agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat
     7801 tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt
     7861 cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt tattatcttg
     7921 aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca
     7981 gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca tctgataact
     8041 tttctttccg atttgcgttt cgagcaaagt caaaaaaaac gagtccaata aaataaaaag
     8101 gtaaaaattt tcaagtttta taaaaaacaa aacatatcag aacaggatcg aatcggtcct
     8161 gttctttttt tgagaacaca aaaagaacaa ataccaaaaa aattttcctg aagttcacta
     8221 tttttatgat acaatatcca agataaggag ggaacatgtt gaaagaatta aaagtaactg
     8281 acctcaaaaa aacttatggt gaaaaagatt tgtttgacca gatttctttt ttggtccacg
     8341 aaaaagaccg tatcgggttg attgggacga acgggacggg taaaacaagt ctcttaaata
     8401 ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt
     8461 accgtattgg ttatctttct caagcagctg aattttccga tgaattaact gttctccaag
     8521 cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga
     8581 tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag
     8641 aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa
     8701 aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac
     8761 gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa
     8821 caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg
     8881 gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct
     8941 tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag
     9001 aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata
     9061 aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac
     9121 ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt
     9181 tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca
     9241 gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg
     9301 aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag
     9361 ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt
     9421 attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag
     9481 aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg
     9541 aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag
     9601 aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg
     9661 atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa
     9721 gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag
     9781 ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca
     9841 gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac
     9901 agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa
     9961 cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga
    10021 atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag
    10081 aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt
    10141 aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg
    10201 gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg
    10261 gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat
    10321 caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa
    10381 taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaagtc cagactataa
    10441 tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga
    10501 atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga
    10561 aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga
    10621 cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc
    10681 tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc
    10741 ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca
    10801 acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac
    10861 tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga
    10921 tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg
    10981 cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga
    11041 agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta
    11101 gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga
    11161 ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa
    11221 tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc
    11281 cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg
    11341 tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct
    11401 ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat
    11461 accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt
    11521 ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc
    11581 atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga
    11641 cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt
    11701 atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa
    11761 caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc
    11821 ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt
    11881 gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag
    11941 atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa
    12001 tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca
    12061 aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt
    12121 ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata
    12181 aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt
    12241 gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat
    12301 gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt
    12361 ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata
    12421 ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa
    12481 gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa
    12541 aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga
    12601 acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa
    12661 gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat
    12721 gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga
    12781 acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac
    12841 agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa
    12901 gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa
    12961 ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca
    13021 actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa
    13081 ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg
    13141 aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca
    13201 aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc
    13261 cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc
    13321 atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt
    13381 attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc
    13441 tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa
    13501 attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta
    13561 tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt
    13621 gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac
    13681 tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct
    13741 aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg
    13801 aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac
    13861 agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa
    13921 tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt
    13981 cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg
    14041 catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa
    14101 caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc
    14161 aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac
    14221 agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga
    14281 agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga
    14341 tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa
    14401 acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc
    14461 cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct
    14521 aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc
    14581 ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag
    14641 tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat
    14701 ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg
    14761 atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg
    14821 ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa
    14881 gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg
    14941 atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat
    15001 ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc
    15061 agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg
    15121 aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt
    15181 cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga
    15241 tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat
    15301 ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc agcaaactga
    15361 tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt
    15421 ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag
    15481 tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc
    15541 agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc
    15601 acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt
    15661 cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca
    15721 gaagccggta aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat
    15781 tatctggaac tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa
    15841 aaatatttag aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa
    15901 gttttttgtc caatagccta attgaaagaa ttatttttat ttcagctatc tagtcgcatt
    15961 tttttgatat ttttcttata ataaagaaag aatgaaaaaa agagggatgg attgatagag
    16021 attgacaaaa aaacaacgct atattaatcg cttctggctt gtattcaaat acttacttgt
    16081 ttctgtattc cttgcgttca tgttgcgggg atttttattc atcccggtac cagtggaagg
    16141 caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa aattttcgga
    16201 gatcagacga tttgatattg ttgtttttca attagcagat ggcacgattt atatcaaacg
    16261 agtaatcggt cttccaggag aaaatgtcag ctaccaaaat gatcaattga agatcaatgg
    16321 gaaagtagtt aaggaacctt atctaactaa aaacctgaaa tcagatcatg caaatgcatc
    16381 ctatacgacg gactttacac tacaagagct aacgggacaa tccaagcttc cggaagataa
    16441 ttattttgta cttggagata atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat
    16501 caataaaact gatattctag gtaaagcaag atttgtctat tatccacttg atgaaataaa
    16561 atggatccag tgagaaacaa taaagcgtgc agtctgttga gactgcacgc tttattgttt
    16621 aatccaaaat cagtttacta ccttagtacg taaaatcaaa cagctcgttt aaacactttt
    16681 ctagcctcta aagttttgat aaatgaataa acaaccatta tttgaatagg gatttggatc
    16741 atattcctga tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa caaaacagac
    16801 atcagccaag aattgataaa aaggctgact gggatcataa tcaataattg ggcagcgatg
    16861 acggaaggca ggtttatctt tttatgatat aaaaataagc cattaataaa tccaccaagg
    16921 aaagccgtga taagaaaagc agcttgaaac gttcctgaac caaatatcgt gaaatttaaa
    16981 gtgttcgaca acatttcaag caaaccagcc cagatcggtc catatagata acccataagc
    17041 gccatactta aaaatccgaa tgtgatttga aaataagcag gaccaatcgc tatcttgtat
    17101 aagatgacat caagggctaa taaaagagca gaggtcgaca acaatcttgc attcatttcg
    17161 ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt
    17221 aataataaaa ggataccata aaatcaccaa tgtgtcatga cacattggtg atttacatac
    17281 actggcatct aacaattaaa aaaagggtga gaagactgct tttataaact gtaaaaaaga
    17341 gattgtacga ataaaaaaaa tagaggcgga gaatacttat cgtatcatcc gcctttgttt
    17401 ttttgttata aaaaacgtca gattagttat ctgcttacat cttcatccat acgggttcta
    17461 atgtatttcc gtctagatct tggacttcta atgaaaacat ttcttcttca ggcattccca
    17521 tatctacttc gtggaaagta ccaccatttt cttttgcaat ttgagcaaat ttttttactt
    17581 catccgcatt ttcaacacta aaagaaatca aagcaccact tgttttttta ttatcagcta
    17641 tttctttatc ttttataaat tttttataaa aatcatgaga gagaagcatt atccaaaaag
    17701 tgtcatccca taccattgaa ccagcttctt cagtagaaaa ttcttcattt tttttaaaac
    17761 ccagcttttc ataaaaagca acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa
    17821 ccatactatt catttgtttc gtctcctttc tgttttgttc gtctatcatc tcttatttag
    17881 gaaacgctgt caaaaattta gattcatata tagaaaccaa aataaaaatt tgcatcatca
    17941 aaaaagtgaa gatgtgatag aatgaggact ggttcacttt cacttaacta atagatggaa
    18001 tttctttata ataaaaaagt gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg
    18061 aatattccgg tatgaaagga atggagagaa caatgaccat ccaatggttt ccgggacata
    18121 tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata tgtggacatc gtatttgaat
    18181 tggtagacgc acgattacct ttatcttctc gtaatccaat gctagatcag atcattcagc
    18241 aaaaacctcg ccttgtttta ttgaataaag cggatctggc agatccacaa cagacaaagc
    18301 tgtggcaaga atattttcgt aaacaaggcc atcttgcatt agctatcacg gcacaagaaa
    18361 gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa
    18421 gagatgctgc tcgggggatc aagcctcgcg ctattcgggc aatggtctta ggtattccaa
    18481 atgttgggaa atctacgttg atgaatcgac tagtaggcaa gaaaattgca cagacaggaa
    18541 ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt
    18601 tagacacacc agggatttta tggcccaaat ttgaagatga agaaatcggc aaaaaactag
    18661 cgctgacagg cgcgatcaaa gaccagcttg tccatttaga tgatttagcg atttacggat
    18721 tagacttttt tgcccgatat tattcaggaa gaatcagcga tcgttatggt ttatcaatcg
    18781 agcaagaaga aaaattggca ccggaattac tgatggatat tacagcaaaa cgtgggtata
    18841 aagaagacta tgatcgtggc agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg
    18901 gacgatacac actggatcgt tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc
    18961 caaagtatca aagaacgatt gaaaagcata cagagaatag atgatcctac actagaatca
    19021 ttaagaaatg atccacgcaa aggcgtgcag caagcactgg cttcttttga acgacgattg
    19081 aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg
    19141 tacgcacaag gtcaccaatg gattgccggc attgatgaag taggaagagg accattagct
    19201 ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg agatcatatt agggctgaat
    19261 gactccaaac aattatcaga gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa
    19321 gccctggcaa tcgggatcgg gatcgttgat cagcagacaa tcgatgatgt gaatatttat
    19381 gaagcaagta aattagcgat gatccaagca gtagaaaaat tgtccattgt accaagctat
    19441 ttgttgatcg atgcgatgac cttggatttg cctattcagc aggaaaaaat aatcaaaggg
    19501 gatgcacgat ctgtgtcgat tgctgctgca agtatcattg ctaaagtata tcgagaccaa
    19561 ttgatgaaag aatatgacgt gctttatcct ggatatggtt tctcgcataa tgcaggttat
    19621 ggaacaaagg aacacttaga aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa
    19681 agttttgcac ctgtaaaaaa atatttacaa taaaaaacag ccgcttttac gtatcttatt
    19741 agaaagagag acgaaaggcg gttttatgat gtatcaaata gaagaaaatt tattgaaact
    19801 tgcttataca aaaggaatca gtagtcaagg aaaatgggca cttgctaact ggatgatgca
    19861 gtatccttat aaagaaatcg gatttgaaga catcattaag ataggtaaaa taaccaaaca
    19921 ccgggagctg tttcgcaaaa gttggcagga aattcacgct aactggggca aaatccaatc
    19981 gaaacagtcg tttatcactt gctttgatcc caaatatccc cctcagctgc ttcacttgac
    20041 ctatccgcca attgtactgt tttataatgg tgatctatct ttactttctt gtaacatgtt
    20101 aagcgttgtt ggtggaagac acacttctgc tttagccaaa aaaacagttt attatttact
    20161 aaaaccagtg atagaagctg gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac
    20221 atacggtcac caagcagcaa tctctcatgg tggacggaca atcgcagtca ttggaacagg
    20281 gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca
    20341 tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag
    20401 aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag
    20461 cggctctctg ataacagcac agcaagcatt ggatcttgga agaacagttt tttctgttcc
    20521 aggaaatatt ttaacagaac attctaccgg atgtcatcat ttgatccaag acggtgcagt
    20581 ttgcacggtt tcgggtgaag atatcttggc agaatttaaa gaataacacc atacttttca
    20641 tttgcttttc ttgacaaacg ttttctgaat ggatatgatt agctacgatt tgtatgaatc
    20701 ctcttttaat agtaggaaca cgacaatgga aaggagtcca ttcagtaatg gcgtataaat
    20761 atttagtgat tgtagaatca cctgcaaaag ccaagacaat tgagaaatat cttggacgaa
    20821 actataaagt aatggccagt gttggccata taagagattt accgaaaagt aaaatgggta
    20881 tcgattttga aaacaattat gaaccccatt atatttctat acgcggaaaa ggcgatgtca
    20941 tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg
    21001 atagagaagg agaagcgatt gcttggcatt tagcgtacct acttgggttg gatctgaaag
    21061 aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc agtcaaagca gcttttaagg
    21121 aaccaagaac gatcgatgta gatttagtag atgcacagca agctcgtcgt accttagaca
    21181 gaatcgttgg ttattcgatc agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg
    21241 caggacgtgt ccaatctgtc gctttaaaaa ttattattga ccgtgaaaaa gagatccgag
    21301 aatttgttcc agaagaatat tggagcatcg acggtaattt taaaaaagct cgcaagaaat
    21361 tcaaagcaaa tttctgggga atcgacggta agaaaaagaa attaccagat gcacaaagtg
    21421 taaaagaagt cactgctaga attgatggaa cagacttcga agttaaaaaa gtagaaaaga
    21481 aagagcgcaa aagaaaccct gcgaatcctt tcactacaag tagcctgcaa caagaagctg
    21541 cccgtaagtt aaactttcga acaagaaaaa ccatgatggt agcacagcaa ttatatgaag
    21601 gaatcacttt gggtaaacaa ggaacagtcg gcttgattac atacatgcgt actgattcta
    21661 cccggattgc cgatacagca aaagcagagg ccgcagcatt tattgaagat acgtatggaa
    21721 aagaattttc tagacatgat caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc
    21781 atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga tgaactcaaa caatatttag
    21841 ataaagatca attgaaactt tatacattga tctggtcacg ttttgtggct agtcagatga
    21901 caccagctat tttagataca atgaaagtga cattagaaca aaatggtgtc atcttcattg
    21961 cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg
    22021 acggcaaaga agagaaagaa aatattcttc cagaacttga agaaggcgat gtagtccaat
    22081 ctgttgatat tgaaccaaaa caacatttta ctcaaccacc agcacgtttt agtgaggcta
    22141 cgttgattcg tgcactagaa gaaaacgggg tagggcgacc ttccacttat gcaccaacac
    22201 ttgatacgat ccaaagacgc tactatgtaa aattgacaca aaaaagattt gagccgactg
    22261 aactaggaga aattgtcaat tcattaatct gtgaattctt ccctcaaatc gtcgatatcc
    22321 atttcacagc agaaatggaa ggagatttgg ataaaattga agaaggcacg gaagcgtggg
    22381 tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa
    22441 aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt cgattgtgat gtctgtggac
    22501 atccgatggt gataaaatta gggaaatatg ggaagtttta tgcttgcagt aattttcctg
    22561 attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg
    22621 aaggacaagt cattgaacga aaatctaaga aaaacagact attctatggt tgttcccgtt
    22681 tcccagaatg tgaattcact tcttgggata aaccagtggg cagaaattgt ccaaaatgtg
    22741 acggatatct ggtcgagaag aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag
    22801 attacgaaga agacatccaa aaataataga aaagaggttc tttcattgaa aatggtaaat
    22861 gttgttggag cagggcttgc cggaagtgaa gctgcttggc agatcgcaca agcaggagtc
    22921 cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg aggcgcatca cacaagtaat
    22981 tttgctgaac tagtatgttc caattcatta agaggaaaca gtttggctaa tgcagtaggg
    23041 ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa tcacttcggc agatgaaaca
    23101 gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt ctttttctga aacaattacc
    23161 cgaaaagtca aagagcatcc attagtaaca gtgataaacg aagaaattac tgaaataccg
    23221 gaaggaatca ccgtagtagc aacaggacct ttgacttctc atccattagc agaaagcatc
    23281 aaagcattca atggttcaga tggtttttat ttctatgatg cagctgcacc aatcgtagac
    23341 aaagctacga tcgatatgga taaagtatat ttgaagtccc gctatgacaa aggagaagca
    23401 gcttacttga actgtccgat gacagaagag gaattcaaag ctttccatga ggcactaatc
    23461 aatgccgaag tggcagaact taaatctttt gagaaagaaa aatattttga aggatgtatg
    23521 ccaattgaag taatggcaca gcgaggattc aaaacgatgc ttttcggacc tatgaaacca
    23581 gttggattgg aagatcctaa aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa
    23641 gacaatgctg cagcttcttt atacaacatc gtgggctttc agactcactt gaaatggggc
    23701 gagcaaaaac gtgttttccg gatgattcct gggttagaaa atgctgaatt cgttcgctat
    23761 ggtgtgatgc accgaaacag ctttatgaat tcgccagaat tgctgaaacc gacttaccaa
    23821 tcaacgaaac gcgaagatct gttctttgca ggtcagatga ctggtgtaga aggctatgtt
    23881 gaaagtgcag cgagcggact acttgctgga atcaacgccg cacgcttagc aaaagaaatg
    23941 gaaccagtcg agtttccaca agaaacagct attggaagta tggcttacta cattactcat
    24001 gcggaaggaa aacatttcca accgatgaat gccaactatg gtctgtttcc tgaactgcca
    24061 gagcgcattc gagacaaaaa agcaagatat gaagcaattg cacaacgagc tttaaaagct
    24121 aatgaggcaa taaaaacaga attagaaaaa actgcaatcc caaatgagta aggaaactcc
    24181 atgaagaggg tgagacggat agtctcgctc tttttcttgc gtttttctta agaatttctc
    24241 aaacaaaatg tgaggaattg cgccaattgt gaacaaactt agaaatattg gttaaattct
    24301 gacaatttat ttgtcttttt ataaacgctg tgctaaatta ttctctggga caaaaggaag
    24361 gtgagaaaat gacagaagcc acgtgggaac aaaaattttt gcgttatttg attgtcgaaa
    24421 gaggatattc agaaaaaacg aaagaagcat atgaagaaga cattctccat ttcaaacatt
    24481 ttttggagga atctggagac gctgatctgc tgaaggcgga atattttacg gttcgtgtct
    24541 atttgagtgc attagctgat catcgtccta gctatagcag aaattctatc agtcgcaaga
    24601 tagccagcct acgttcgttt tatcagtatc ttctcaaaga agaagtgata aaagaaaatc
    24661 cattttctta cgttcatctc aaaaagaaaa atttgagact gccacgtttt ttttatgaaa
    24721 atgaaatgca agcacttttt gacagcgtag caggagatag tcctttggat ttgaggaacc
    24781 gcgctttact ggaagtcctt tacgggtcag gcatccgttt aagtgagtgt agcagtttgt
    24841 cggtatcaga cattgatttt gattcagagg tcatgttgat ccatggtaaa gggaacaaag
    24901 agagatatgc tcctctaggt tcttttgctc aagatgctct gcaagaatat ttcagggaag
    24961 gccgccaggt attgatgaca aaatatcacg aagagcatga tatcgttttt gtgaatcatc
    25021 atggaaaacc aatcacgcct actggaattg aatatgtctt gaatcaaatg atcaaaaaaa
    25081 gcagtttgga tagtgatatc catcctcaca tgctgcgcca tacatttgct actcatttat
    25141 taaataatgg ggcagatttg cggacagtcc aagaattgct aggacatgcc aatttatcga
    25201 ctacccagat ctatgcccat gtaacaaaag aaagtttaca aaaaaactat cgttctttcc
    25261 atccaagagc gtaaaaataa ggaaaagaac aaatttagga ggaaacaaac atggtcgaat
    25321 caccatttca ttcaaccact atctgtgccg tcgaaaaaga cgggaaattt gctatggctg
    25381 gagatggcca agtaacaatg ggtgaatctg ttgtcatgaa aggcactgca aagaaagtac
    25441 gacgtatcta caatggagaa gttgttgtag gtttcgccgg aagtgttgca gatgcgttta
    25501 ctttagaaga aaaatttgaa ggcaaactaa acgaatataa cgggaatcta caacgcgcag
    25561 ctgtcgaatt agctcaagaa tggcggacac agcaatctat gcaaaaactt gaagcgatgc
    25621 tgatcgtaat gaatgataaa gaaatgcttc ttgtttcagg tacaggagaa gttatcacac
    25681 cagatgatgg tatcttagct atcggatcag gagggaattt tgcgttggct gcagcccgag
    25741 caatgaaaaa ctatgctcaa aacgacatga gtgcgaaaga aattgcagaa aatgcgttaa
    25801 atattgccgc agatatctgt atctttacta atcataatat tatcgtagaa gaactgtaag
    25861 aggtgagtag atatgtatga attaaaacaa acaccaagac aaatcgtaag tgaattagat
    25921 gaatatatcg taggacagca cgcagcaaaa aaatctgttg ccgttgcttt gcgtaaccgc
    25981 tatcgccgtt tgcagttaga tgaaaaaatg caacaagata ttacaccaaa aaatatgctg
    26041 atgatcggac ctacaggggt cggtaaaaca gaaatcgcac gacgtttggc aaaaatcgtc
    26101 aatgcaccat ttgtcaaagt cgaagcaaca aaatttactg aagtcggtta tgtcggaaga
    26161 gatgtagaat caatggttcg ggatttagta gaaaatgcga tccaaatcgt agaaaaagag
    26221 cagtacagcc gtgtatacac aagtgctaaa aaaaatgcag aaaaacgttt ggtaaaaatc
    26281 ttggtacctg gtatcaaaaa agaacagaaa caatccacta atcaatttga acaaatgatg
    26341 aatcttttta atgctagtca gcaaaatacc gctgaaccgc aagaagaagt gacagaagat
    26401 attcgaatca atcgccgtac agtcttggaa caactacaaa agggtttact ggatagtcgt
    26461 gaagtaacca ttgaagttga agaccaaaag aaacctgcac caatgatgaa caatggattg
    26521 gagcaaatgg ggatcgacct aaatgaaact ctaggtgctt tgacaccgaa gaaaaaagtg
    26581 gaacggacgg taacagtcaa agaagcttta gagttattga tcaaggaaga atcagcaaaa
    26641 ttagtcaagg atgctgatat ccatagtgag gcaatcaaat tagcggaaga aagtggaatt
    26701 atttttatcg atgaaatcga taagatcact tctaaatcac agcaatctgg agaagtatcc
    26761 agagaaggcg tccaaagaga catattgcca attgtcgaag gttcacaagt aaacacaaaa
    26821 tacgggatca tccaaacaga tcacgtccta ttcattgctt caggagcttt ccatttgtct
    26881 aaaccaagtg atctaattcc agaacttcaa ggacgcttcc cgatacgggt agaattagat
    26941 gatttgacag ctgaagattt tgtgaagatt ctgaccgaac caaataatgc tttagtaaaa
    27001 caatacgtcg cgttgatcgg tacagaaaat gtgtcagtta cgtttacaaa agaagcaatt
    27061 gaacggatcg ctcaaatcgc tttcgatgtc aaccgtgaca cagataatat cggcgcacgc
    27121 agactgcata ccattttgga aaaactacta gaagatctat tatttgaagc accagatatg
    27181 caaatgggag aaataacgat cacagaagca tatgtggatg acaaacttgg ctcaattgcg
    27241 gcaaatgaag atttaagtcg ctatatttta taaaaaatcg gaggacgaca atgacagact
    27301 tgctaacgaa aacccgcaag ataaacacac ttttgcagca aaagaatact tttgacatgc
    27361 aagcagattt gccttacgat aaaatggctg tcacactagg tggtattctt gacagcaaca
    27421 cctatatcat aggaagtgac ggagtattac taggttataa tgaacgtcac gatgtgaaca
    27481 atgaacgaat caaaacaatg tttgttgaga aacaatttcc tgaatcttat actgatacag
    27541 tggcacagtt gatgaaaact gaagctaata tcccaattac tagtgatatt actgcatttc
    27601 cggtagagtc aagagagaaa tatccatttg gtctgacgac tgtcgttccg atctttggag
    27661 ctggcgaacg tttgggtacg attatcttat ctcgtatgga acaatcgttt gcagatgacg
    27721 atctagtctt agcagaatat ggagctacgg ttgtcggtat gcagatcctt tatcagaagt
    27781 ctcgaagcat cgaagcagat gtacggagtg caacggctgt ccaaatggca atcaacacat
    27841 tatcttatag cgaattgaag gctgtacaag ctatctttga agcattggat ggagatgaag
    27901 gtcgtctgac tgcttctaat attgccgata gtatcggcat cactcgttct gtgatcgtca
    27961 atgctttacg aaaattggaa tcagctggaa tcattgaatc tcgttcgtta ggcatgaagg
    28021 gaacttattt aaaagtattg aattcccgtt ttaaagacga gttagcaaaa cacagttatt
    28081 agaagtaagg ggactggggc agatagaaat gtttgttcca gccttcccat tttaaaaagt
    28141 aagtcgaagt tggaggaatg gcaatgactg ttattattca aaatgaccag ctgatcgccg
    28201 aaatcgctga acacggtgcg gaattgatca gtctgaaatc aaaagaacat ggtttagagt
    28261 atatctggca aggtgatcca gcgtattggg gcagacatgc accggtttta ttcccttttg
    28321 ttggacgttt aaaagatgat cagtatgttt atcaagggaa aacatatcaa atggggcaac
    28381 acggatttgc tcgggatatg gattttgaag tcattgaaca tggacaagaa atggctagtt
    28441 tcatcttaag gagtacgccg gagacattag aaaagtatcc atttgatttt gaattagtga
    28501 tcagctatga attaggagga gacggcatca cggttcatta tcaagtggca aataccggtg
    28561 aaaaagaaat gttcttttct atcggtggac atcctgcttt caatgtacct cttgaagaag
    28621 gattgtcatt tgaagatttc tatctggcat tttctccatt gaaatcaaga ttacgcctac
    28681 cattgaaagg cccattcatc gatatggatc aaaagacgtt aggtcagaca aatacgaatc
    28741 tggctttgaa tcgtgaactt tttgagcaag atgccttggt ttttgaaaca aggggtctaa
    28801 atgcgtacgc cattcgaagt gaaaaatctt cccatagcgt aactttaagc tataaagata
    28861 tgccttatgt agggatctgg tctccttatc caaaagaagc gcctttcgtt tgtatcgaac
    28921 cgtggtgcgg aatcgctgac acggtgaaca gtagtggaaa cttgatggaa aaagtaggga
    28981 tcaatcaact aggtgcccat gaaatcttca agacaaaata ttccatcaca gtgaagtaag
    29041 aggttgtgac cgagaagtta gcagggaaca ccgtttacca aggtgtgtaa aaaagctatc
    29101 ctgtaagtaa taagggagtg tgacatcgat ttgtcacact cccgttttat ctctttggaa
    29161 tgaaaaagta tcaggatttt ttcttgctgc tattcaaacc aaacgggatt cgactttcag
    29221 tatggtttct gatacgcttg atattgtctt tatgccgata aaagataaag gaagaaacta
    29281 aaaaggcaat gatcgtcagt aaccaattgg gcttagccaa aatcacagga gctacataag
    29341 gaaggacgat cgtagataaa gtgatcaata ctgcactgat catactgctc aaactcacca
    29401 tacttgtaca aaataaacta acgataaaga tgatacaaga ataaacaaaa aatacaggat
    29461 taaatcctaa aagcattcct gcacttgtcg caacagcttt tcctcctttg aacccagcaa
    29521 aaatcggaaa tgtatggcca agaatggcgc ataccccaaa ccaaagaggg ttaatcccag
    29581 aaaggtggaa gatcatcggc aaacaagtag ccaatgtacc ttttaaaata tccataagca
    29641 ggacaacaat cccggctgtt ttgcctaata cacgaaatgt attggtcgtt ccagtattac
    29701 cgctcccgtg ttgtcggata tcttttttga aaaacaattt gccaatccag acaccggaag
    29761 gaatagaacc taaaagatat gctaaaataa gtaataagat caatttcatt ttttaccctc
    29821 tttcaagttg aaaactatcc tttattttag catgaatagg taacaggaac aattgaaaaa
    29881 atgaaagata caagtataat agatggaata aaaagaaaag caaggggtgg cagaaatgtc
    29941 tgtaaaaaat ccaacacaag aaacgatttt caactatttg gaagaagcaa aaaatattgc
    30001 cgtcgtaggc ttgagcaata aaccagaacg aacaagttac atggttgcac aggtattgca
    30061 agacaatgga tataaaatca taccagtaaa tccattattg gctggtgaag aaattctagg
    30121 cgagaaagtc tatgcgaatt tgacagaaat cgatgatcca atcgatatcg tagatgtatt
    30181 tagacggagt gaatttttac ctgaaatagc aggtgatttt ttgaaaactg atgctaaagt
    30241 gttctgggca caattaggga ttgaaaacaa agaagcagct gagatgctaa gaaaagcagg
    30301 acgtacagat atcgtaatgg atcgttgtat taagattgaa cttggtaagt tgaaagaaaa
    30361 ccaaaaataa aaatgaccac aaagaagaga tgatcgatag aggggaatgt cgaaaaagcg
    30421 gttaggagat aattaaaatt gatttctcgt ctaccctctt ttttttcacg accttttcta
    30481 gtatgataat cgtggtgcgg ttttgccgca aagtattatt gaaggagcgt ttgccttggc
    30541 aaaaaaagta aacaacgaat acaatgactc gtccatccaa gtattggaag gattagaggc
    30601 agttagaaaa agaccaggga tgtacattgg atccactgac agccgaggct tgcatcattt
    30661 agtctatgaa atcgtcgata atgcagttga cgaagcatta tcaggttatg gcagtgagat
    30721 tgatgtgacc atccatgaag acaatagtat aactgttgca gatagcggac gtgggatgcc
    30781 tgtaggaatg catgcttccg gaataccaac agttgaagtg atttttactg tgctgcatgc
    30841 tggcggaaag tttggtcaag gagggtacaa aacttctggt ggactgcacg gtgtaggagc
    30901 cagtgtggta aatgccttat cgaaatggct aattgtaacg atcgttagag atggcgtaga
    30961 gtatcagcag aaatttaaaa acggcggtaa acctgacggt actttgaaaa agataggaaa
    31021 aacaaaaaaa gcaaacggga cgactgtcca ttttttacca gatgatacaa tattcagtac
    31081 aacgaaattt tcatatgaaa ttttagccga acggttaaga gaatctgcct ttcttctaaa
    31141 aggcgttaaa atctctcttt ctgatcttcg tggagaagaa ccagtcaagg aaatttttca
    31201 ttacgaagaa ggaatcaaag aatttgttga ttatctgaat gaagaaaaag atacattgac
    31261 accagttgtt tatttttcag gagaaaaaga aggtatcgaa gtagaggtcg cttatcaata
    31321 caatgacggt tattcagaaa atgttttatc ttttgtcaat aacgttcgga caaaagatgg
    31381 gggaacccat gaagctggta tgaaagcggc gatgacgaaa tcttataatg aatatgcgag
    31441 aaaagttggt ttgctgaaag aacgggacaa gaatctggaa ggtagtgatt tcagagaagg
    31501 gttagctgct gtactttcta tccgtgtacc tgaaaatctt ctgcaatttg aagggcaaac
    31561 aaaagaaaaa cttggtacgc cagttgctcg taccgtagta gataacgtca tcagtgaaca
    31621 aatgggcttt tatttgcagg aaaatagcga aatgagccag atgttggtcc gtaaagcaat
    31681 caaagccaga gaagcgagag aagcagcacg taaagccaga gaagaaagcc gaaatgggaa
    31741 gaagcgtaaa aaaggcgaat ctcttctttc aggaaaattg acacccgcac aatcccgaaa
    31801 ccctaagaaa aatgaattgt atttagtcga aggggattct gctggcggat ctgctaaaca
    31861 aggaagagac agaaagttcc aagcaattct acctttacga ggaaaagtca tcaataccga
    31921 aaaagctaaa atgcaagata tcttgaaaaa tgaagaaatc aatacgatga tctatacgat
    31981 tggtgcagga gtcggtcctg agttttctat cgaagactgt aattacgaca aagtaatcat
    32041 catgaccgat gcggatacag acggcgccca tattcaagta ttactgctta ctttctttta
    32101 tcgttatatg aaaccgctga ttgaagctgg gaaagtatat atcgcgttac cgcctttata
    32161 taaagtatcc aaaggacaag gaaagaagca agtcattgaa tatgcgtgga cagatgacga
    32221 attggcagca atgattaaaa aagtcggcaa aggatatatg ctgcagcgat ataaaggact
    32281 tggagagatg aatgcggaac aattgtggga aacaacgatg gatccgactt cccgtacgtt
    32341 gatccgcgtc cgtatcgatg atgctgcgca agctgaacgt cgagtaacca cactgatggg
    32401 tgacaaagta gaacctcgaa gaaaatggat cgaaaatcac gtgcaattta cgctcgaaga
    32461 agatggcagt atcttagata aaaaagagga tactgaaatc tctccttcag tttctaatga
    32521 tttattagat gaagaacgag cagataaaaa tgaaaacaat cagttatttg aagtagaata
    32581 ggaggtcacc tgatggaaaa tcgacaagaa gtacaagaat taactttaga agaagtgatg
    32641 ggtgaccgct tcggacgtta ttccaaatat attatccaag aacgtgcact gcctgatatc
    32701 cgtgatggat tgaagccagt gcaacgccgg atattatttg ccatgaacaa agatggcaat
    32761 acgtatgaca aaggattccg taaatcagca aaatctgtcg gaaatatcat ggggaattat
    32821 catcctcatg gtgacatcag tatttatgaa gcgatggtcc gcatgagcca agactggaag
    32881 ttgcgggaag tactgatcga aatgcacggg aataacggga gtatggacgg tgatccacct
    32941 gctgcgatgc gttatactga agccagatta tccaagttaa gtggagagat gctagcagat
    33001 atcgagaaag aaacagtcga tctcgtgtgg aattttgatg atacagaaaa agaaccaaca
    33061 gtccttccag ctcgataccc aaaccttctc gtcaatggat cgacaggtat atctgctgga
    33121 tatgcaacag aaatcccgac tcataactta gctgaagtga tcgatggcac aatctatatg
    33181 atcgatcatc cacaagcgag ccttgaaaaa ttgatggaat atattcctgg acctgacttt
    33241 ccaacaggag ggatcttgca aggaaaagat gaaatcaaaa aagcttatga aacaggacga
    33301 ggaaaagtta ttcttcgttc gaaaacaaag atagaggcaa tgaagggtaa taaacagcaa
    33361 atcgtgatca cagaaatccc atatgaagta aataaagcga ccttggtgaa aaaaatggat
    33421 gagatccgtt tgaataagaa aatcgatgga atagctgaag tacgagatga aagtgataga
    33481 acaggtctgc aaattgtcgt cgaattgaaa aaagacgcca atgctcaagg aattttgaat
    33541 tatttgttca aaaacactga gctacagatc aattacaatt tcaatatggt cgctatcgac
    33601 catatgactc cgcatcaagt tggactgaaa gacattttaa gaagttatat cgagcatcga
    33661 aagcaagtca taacaaaacg tagtcaattc gatttagcca aagctcaaaa aagacagcat
    33721 atcgtagaag gattgatgaa agctttatcg attctggatg aagtaatcgc aacgattcgt
    33781 gaaagtaaag acaaaaaaga cgccaaaaag aatttagtcg acgtctttca atttacagaa
    33841 gaacaagcag aagcaattgt cacattgcaa ttataccgtt tgacgaacac ggatatcaca
    33901 gagcttcaaa aggaatccga aagcttgatt gcgcaaatca cagagttgaa taaaatcctt
    33961 tcaaatgaca aagagctttt ctctgttatg aaaaaagaat tgcgagaagt gaaaaaaaat
    34021 tattcttccg ctcgtctaac gacaatcgaa gacgaaatcg aagaaatcaa aattgatacg
    34081 caagtcttag tggcacaaga agacgtaatt gtcagtgtga cgcgagaagg ctatgtgaag
    34141 cgaacaagcc ttcgttctta tagtgcttcc aaaccagaag aaatcgggat gcgagaaggc
    34201 gactacttgc tatactcagg tgaattgagt acattggacc atgttcttct gatcacaaat
    34261 aaagcaaatg tgatctaccg ccctgtccat gaactacccg atttgaaatg gaaagatgct
    34321 ggtgagcaca tttctcaaac aatctccaat ctgtctgtag acgaatcgat tttagctgtt
    34381 ttcccatatc aaaaaattca agcagagaaa acatttgtat tcatcagcaa gaatggattg
    34441 atcaagcaaa cacgaatgac agagttcgaa ccatggcgca cctataagag ccgtccgctc
    34501 agctgtatga agctaaaggc ggaagaagat gaactgattg ctgtgtatct gaaagaagat
    34561 cagccaggct tagatgtttt cttagtaacg catcaaggaa tgggtttgcg ctatccttta
    34621 acagatgttc ccgttgttgg agcaaaggca gctggagtga agtctatgaa tttgaaagat
    34681 ggagatagga ttatcaacgg gctgcttgtg ttagcagaag gtgatacgcc gatcgtaatc
    34741 gttactcagc gaggagctgt taaacgcatg cttgctcaag aaatcagcca aacttcacgt
    34801 gcgaagcggg gagtgacggt catgcgtgag ctgaaaaaaa atccgcaccg tattatctat
    34861 atgtcggaag gaagatcaaa agaaataacc ttgatgaatc aaaaagggca gcagttaacg
    34921 attgacccaa ctgatttccc tataggtgat cgaacatcga atggatcatt tgcgatggat
    34981 gagaaaaaag gtggagaagt catcgaagtt atcgatgctc ctgagatttt catcgagtcg
    35041 taactgttgt agtattttaa tgaagagacg ccatctgtat taatacaggt ggcgtctctt
    35101 tttcattcat ttttaataaa tccttatacg tttgcaaaaa aagatgaaag tgttcacaat
    35161 gcttaaatgt tggtatgtca acgttgattt aaatatatgt aaataaaaca taaaaattgt
    35221 taaataaaaa acaagaaaac ccttgcaaag atagggtgct gtgttatact aatcacgtaa
    35281 aaactgttac ataaaacaga aatttaggag gtccatacaa tggagcaatg gaatggattt
    35341 aaaggtacca aatggaaaga aggcgtagac actagagact ttattcaaaa taactacact
    35401 gaatatcgcg gtgatgacag ctttttggaa ggcccaacag aagcaacaga aaaattatgg
    35461 gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta acggtgtcta tgatatggac
    35521 acagaaatcg tatcaactat tacttcacac gatgcaggct atttggataa agatttggaa
    35581 aaaatcgtcg gtctacagac agacaaacct ttgaaacaag cattcatgcc gttcggcgga
    35641 atcaatatgg ctaacaatgc gctaaaaagc aatggctata caccaaacga tgaattgaca
    35701 catattttca ctgactggcg caaaacacat aatcaaggtg tatttgacgc ttatactgct
    35761 gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc ttccagacgc ttacggtcgc
    35821 ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg gaatcgacta tttgatggaa
    35881 caaaagaaaa aagatcatga tatgactggt tatcatacaa tgagtgaaga agtgattcgt
    35941 ttacgtgaag aaatcactga acaatatcgt gcattagcgc aaatgaaaga aatggctgcg
    36001 aaatatggct atgatatttc taaaccagct gcaaatgcaa aagaagctgt ccaatggcta
    36061 tacttcggat atttagcagc aatcaaatca caaaatggtg ctgcgatgtc aatcggacgt
    36121 atctcagcat tccttgatat ctatatccaa cgtgatttgg ataatggagt aatcacagaa
    36181 aaagaagcac aagaattgat cgaccactta gtaatgaaat tacgtatggt caaattcgcg
    36241 cgtacacctg aatataacca attattctca ggattcccta tctgggctac tttatccatt
    36301 gcaggtatgg gattagatgg tcgttcatta gttacaaaga atgatttccg tctgcttcat
    36361 acattaacaa atatgggacc ttcaccagaa ccaaacttga ctgttttgta ttctgaacac
    36421 cttccagaag gattcagaac atatgctagc agaatcgcta tcgaaagttc ttcgattcaa
    36481 ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg atgattgtgc gattgcttgc
    36541 tgtgtatcag gaacagttgt cggaaaagac atgcaattct ttggcgctcg tgctaacttg
    36601 gctaaaacac tattatatgc catcaatggc ggagtcgatg aagtgactgg cgcccaagtt
    36661 ggaccagaat atcgtccaat cacatcagaa atactagact ttgaagaagt gaaacataac
    36721 ttcatgaata tgatggattg gttagcagaa ctatatgtca atacgttgaa catcattcat
    36781 tatatgcatg ataaatattc ttatgaagca gctcagctgg ctttgatgga ttctgacttg
    36841 caacgcacat ttgctacagg tattgctggg atctctcatg cggcagacag cttgtcagcg
    36901 atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag gaatagcagt tgattttgaa
    36961 gtggatggcg acttccctaa atacggaaat gatgatgacc gtgcagatga attagtttca
    37021 tggatcttgg aatacttcat gggacaaatc aaacgtcata aaacttaccg caactcaaaa
    37081 ccaacaactt cattattgac gattacttca aatgttgttt acggtaaaaa tacaggaaat
    37141 actcctgatg gccgtgaagc tggaaaacca cttgcgccag gagcaaatcc aagttacaat
    37201 gcggaacaaa gtgggttatt agctagcctg aactcaacag ctaaaatgaa ttacggctgt
    37261 gcattggatg gaatttcaaa tacccaaaca atcaatcctt caggtcttgg aaaagaacaa
    37321 gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat acttctcaaa aggagcttat
    37381 catttgaatg tcaatgtatt cagtaatgat ttattgcgtg atgcaatgga aaatccaatg
    37441 aaatatccga acttgacgat ccgggtatct ggttatgcag ttaaattccg tgacttgaca
    37501 agagaacaac aattagatgt cttgatgaga acttcacacg acagaatgta aaaagttcgt
    37561 gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat agccatcgca tcaaaatcga
    37621 ataatcagta tgcttatttg aaagaaacga tttacagggg caggacttgt atgttccgtc
    37681 cctgtaaatt ttacacataa cgaagcggca attgcgaaaa attttgttca gaaagcttcg
    37741 cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa agatgtgaac ttaaatggaa
    37801 gaaaaaacaa tcggctatgt tcattctatc gaaactttcg gttcagtaga cggacctgga
    37861 ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt gccaattttg tcacaatcca
    37921 gatacttgga atattggcgg agggaaagaa tacacagcag atgaactttt agataaagca
    37981 gaacgcttca gaccttactg gggagataaa ggtggtatta ctgttagtgg tggagaacct
    38041 ctattgcaga tcgatttttt gatcgaacta tttaaaaaag caaaagagcg gaagatgcat
    38101 actacattgg atacatgcgg aaaacctttt acgtatgaag aacctttctt ttcacgtttt
    38161 caagaattga tgaagtatac agatttactg ttatttgaca tcaaacacat cgataatgaa
    38221 gagcataaaa aactaacgca ttgggacaat gaaaatattt tagaaatggc tcaatatttg
    38281 tcaaaaatca acaaaccggt ttggattcgc catgtactag ttcctgagcg aagtgattat
    38341 gatgaatatt tgatccgact agacaacttt attcaaacct tgtcaaatgt agacagagta
    38401 gagattcttc cttatcatac gatgggaaaa tacaagtggg aaacacttgg tttgaaatat
    38461 ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg aaaatgctaa acgattgctt
    38521 catacagaag agtatacaaa atatttaacg cgataggtcg catcggaaag aagataagta
    38581 ttgcttcttt ccgttttttt gctattatta aataggagaa atcacaaagg agcgaatgac
    38641 atgtcaaaaa ttttagtttt tggtcaccaa aatccagata cagatgcaat cggggcagcc
    38701 attgcgtttt cacacttgca aaatgaatta ggtaaagaaa cagaagcagt tgctctaggt
    38761 acacctaatg aagaaacaca atacgcatta gaccactttc agctatctgc accacgagta
    38821 gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg tggatcataa tgaattccaa
    38881 caaagtattt cagatatcag cgaagtaaat attttagctg tagtagacca ccatcgtatt
    38941 gcaaactttg aaacagctaa tccattgtac tatcgtgcag aaccagttgg atgtacaagt
    39001 acgattattt taaaactata taaagaaaac caagtaacaa ttcctaaaga aatcgcagga
    39061 atcatgctct cagctatcat ttcagataca ttgttgttca aatcaccaac atgtacgcct
    39121 gaagatgaag cagctgcaaa agaacttgca gcaatcgctg aagtcgatct aaacacttat
    39181 ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata aatcagctgc ggttcttctt
    39241 gatttggatg caaaaagttt cccaatgggc agttccaacg ttcgtatcgc tcaagtcaat
    39301 acagtggacc ttaatgaagt gatggatcga caagcagagc tagaagcagc aatggaagat
    39361 gaaatgaaag caaatggcta cgatctgttc gtattagttg tcacaaacat cttagacagt
    39421 gattcagaat tacttgttgc tggtaaagga aaagaaaaag tagaagaagc tttccaaaca
    39481 actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt cacgtaaaaa acaagtcgta
    39541 cctcaattga cggctgtatt tagctaatca aatggtagta tgaagcattc aaaatgaatg
    39601 gataagcgat tgatataaaa agaaacatag aacatactaa aggccgaaga aatggaggag
    39661 aataacagtc aattatgact cttgttcttc ctccttttta cgtaaaagcc ttttaagtaa
    39721 aataaaacgg atagaaaaga gaacatatat gaactattat tcaaaaaata aggaaaactt
    39781 ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg accgattatc tatatgaaca
    39841 tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa ttttcgcaaa ataaattttc
    39901 ccattttctt gatttattga tcgatgcagg attaatcaag agggaagaac gaagatatca
    39961 tttgaacttt ccaatatttg atcctaaaga ttacttgcaa caagccactt cggctgctga
    40021 aaccatagct gaacaattga agagactctc tgttgatgaa caaaaattag cgatgggaga
    40081 agttatttgg gcgtattgtt tcgaagatga acggaaagaa gcgtattttt atggagtaag
    40141 aaacagccga gagacagaat tgctgaggac aactgcagga aatgagaagt accgttttat
    40201 cacactttcc tctatagaac acttcccgct tactttggct aattactttt ttgtacagaa
    40261 gaatcagctt cctgtaacga aggcattcaa agaattagcc gaacttattg gagacgtaaa
    40321 tgaatcgtac ttttttgatc aaatagaagt tatcgttgac aggatacgta agaataaata
    40381 taaaaatcgc cggcctagta ttttccatca gtctctactt gtgacgaata ctataaagga
    40441 agaagaatca ttcacattag aattacctat tgtcgaaaaa aacaattttg agattgaatt
    40501 accaacattg gatccttcat tgacaatgga ggaaatggcc tttcttaaac gacaaatctt
    40561 ttctgagctg agcaagaaat ttataccgca tgcattttct tatatcaaag aatatggaac
    40621 agttttagtg agtaaaacct aagagagcag taagcaaaaa ttatactgac tcttgcagga
    40681 ggagcctttc tcttccttta tggttaaaag aattatttga gtattttgcc tatctaccac
    40741 ctattagatt cactacatgg tctggaaaca aaggaatgca aaaaaatggt gagaaatgtg
    40801 gaatgacaaa ggaagtagtg ataagaaaag ttagattttt aaataatcaa tattatgatt
    40861 cagtcaagta tggcatttta tgggaagaac tagctgatta gattaggaag tccatttgat
    40921 aatccagaat cactaagaaa cgaatggcga gtcattaata atttatccaa tcttgggtta
    40981 ttcttttaga ttgaagtgtt taaaactaaa aaagcattta tgaagtcgta atagtcaggc
    41041 ttcaaaatgc tttttatttt gtttgttttt tctttgccac ataaatacac ctagactgac
    41101 gatgaagagc atatccaaca gatttcctgc gacttggatc gtcgaagggt tggtcagtgg
    41161 cattaaagca aggacaaaaa aggaaaacaa gctgtaaata aaacaggtga caggaactaa
    41221 tcgaaagaag agcggacatt tcaattggct aaaaatgagt gaaaaaataa aataagtaaa
    41281 aatggataaa atcacgacta aaccattcat gataaagaag aaaagcgtat tgtgattaaa
    41341 cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat gcaatcaata cgcttgaagc
    41401 ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca ccgaaataaa cagaaaacag
    41461 taagccgcaa agagaagaca aaatcaaaaa aaacggattt tcttcggctt ctgtgatatc
    41521 agaatgatcc aaaagcaacc agcctaatgc actagaaggc gcatgtgagg tcttaaggta
    41581 tgaataacat agaaaaaata agatcgtcaa agttgcaaaa aaagagccta aatgtaaaaa
    41641 attagtggtt ctacgttgaa tttccataat atctccaaat taaattgata agtatataaa
    41701 gtataataac aaattgatga gtatatgtcg atgtttttta atcaaaataa taaataaaaa
    41761 ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg tgagttattt
    41821 tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt ttggctcata
    41881 agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt cacgttcgct
    41941 ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca ttgcagaagt
    42001 ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat cttctgcgct
    42061 atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt cttcaatcaa
    42121 aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat tcagtggatt
    42181 atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct cgaccatact
    42241 gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta acgtgttcag
    42301 agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat caatcatttg
    42361 taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg aagaaaaatt
    42421 gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata aagataggtt
    42481 aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg ctaagtctcc
    42541 ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta gagaacattg
    42601 tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt ttgatactgt
    42661 ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt tatccactcc
    42721 tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc gagccattca
    42781 gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt ggagcatggt
    42841 tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat ccaattatat
    42901 tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa ttaagagagg
    42961 aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag ttagttttga
    43021 cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat gcgatgaatc
    43081 atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg gatagtatct
    43141 acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga ggtcttctac
    43201 atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc gccttgttgg
    43261 ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca cctttggaat
    43321 atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca aaagactatt
    43381 ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa aagttagctg
    43441 gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat atacaaacgg
    43501 aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt tttcgttcct
    43561 caaaaacaca gcgacatatg gtacaaatac cactaatgga agaagcttcc acttttttag
    43621 caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat gacgatagag
    43681 gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt ggtataattg
    43741 atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag cagatgttgc
    43801 aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc cggaacaggt
    43861 gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt accgtccaaa
    43921 tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat ttatactgga
    43981 agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg ctaaacggat
    44041 cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg tcggttcttg
    44101 tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg accgtttgga
    44161 aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag acagcgataa
    44221 tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc aaacgattat
    44281 ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg gttatttatc
    44341 tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc attcgacaga
    44401 agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt gttttgtatg
    44461 tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg gaggacactt
    44521 gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa tcagttcacc
    44581 taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg gagaaatgtt
    44641 gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt ttcctgaact
    44701 agttatcaga gagacaacga attaagtctt attactatat atatatttat gaaattcatc
    44761 gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg tgctattatt
    44821 gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac gaaaacattt
    44881 ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca agaagcgagt
    44941 attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt ttgaacagat
    45001 gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc gtcgtgcaac
    45061 aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag gaaaattacc
    45121 ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa aactttttgg
    45181 tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc atactacacg
    45241 aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta aatatgtacc
    45301 cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt taatcgaatc
    45361 gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc cgtatcaaga
    45421 aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt tagaggaaac
    45481 aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac gctcattaca
    45541 aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta aataagtgat
    45601 atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc cttattttat
    45661 actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc gaattttttc
    45721 tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg cagatcgttt
    45781 gcaaaaatag tttatttttt tataaagaag attttactga ataatatcat tttagtttac
    45841 ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt gctctaactg
    45901 ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca attggggcaa
    45961 aacagatcca atttccagat acttcacttt attcaatgta taagatagaa tagaacagct
    46021 aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa ctaaaatacc
    46081 aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt caatggcttg
    46141 ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat
    46201 gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag caaaatgtat
    46261 atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga cgatcgtccg
    46321 gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta aattaaaaag
    46381 cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag caataccagt
    46441 aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta atccgtagat
    46501 tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt tcatttctag
    46561 cctcttttct tttgaaaatt ttcagcaact tctttattat actgacttat actcttactt
    46621 caagaggaaa atgaaaaaaa attatttttg gctgtttctt tttatttatc aaataaggag
    46681 tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca aaaaaacaga
    46741 gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa tggtccagat
    46801 aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac ttgctatgaa
    46861 agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc agtcaaaaga
    46921 agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga tataagtaaa
    46981 aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa
    47041 agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc tcccccacga
    47101 gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga
    47161 tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta gtgaaaaaac
    47221 gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca gcatatttag
    47281 aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg
    47341 aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc atgaataatt
    47401 ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt caacatctgt
    47461 ccatttctct catttctttg ctgattgcag cactgattgc tattccttta gcgatttggg
    47521 cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg ctccaaacga
    47581 ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga actgttccag
    47641 cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg tatattggtc
    47701 tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg tccagaatga
    47761 gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca ggtatccgaa
    47821 ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga gcaggtggat
    47881 tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca ttgattggtg
    47941 cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg ttgcagcata
    48001 agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt ggcggcgttt
    48061 ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga aaattaggag
    48121 cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac acaaaaacag
    48181 aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca ctggatcaag
    48241 gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg gtgaaggtac
    48301 ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa gcagaagaat
    48361 tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa aatacgtatg
    48421 ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt tccgatttgg
    48481 cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat cgttcagatg
    48541 gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa agcatggagc
    48601 caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat gcctattcca
    48661 cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga gggttattcc
    48721 cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat cctgaaatcg
    48781 ttaaagcact gaataaatta agcggaaaaa taacagagga tcaaatgatt gatatgaatt
    48841 accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg
    48901 aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac atgtgcaaaa
    48961 aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat
    49021 ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg
    49081 attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa atgaatataa
    49141 tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt tgttcccgac
    49201 catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa
    49261 aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg atatttatcg
    49321 agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga tcatccgagc
    49381 tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat tagatccgat
    49441 ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg gcacgaccat
    49501 cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta ttgcatttat
    49561 gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga atcctaaaaa
    49621 cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc gagtagaaga
    49681 tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc aacaagtttg
    49741 ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa gcattgtgat
    49801 agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg cttttttaag
    49861 tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca ggtgtgagcg
    49921 gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg gtcgaagaag
    49981 atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg
    50041 cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac gagatcccaa
    50101 ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca gtcccagaaa
    50161 gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt
    50221 tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac ttagttttgg
    50281 caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg aaaactagtg
    50341 cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt ggtggttata
    50401 ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc attgtccatc
    50461 ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc gtccatcaaa
    50521 tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc atttcagaag
    50581 gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg attttttgtg
    50641 cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat atcgtctttg
    50701 ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa atttttgcgt
    50761 gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact tttgaaggaa
    50821 attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat ccgattatac
    50881 ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa agtcacgcct
    50941 cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat catcgtgtca
    51001 atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg attggtgctg
    51061 cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc atcaatcaaa
    51121 aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg gcaagtgatc
    51181 tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt tttccttctt
    51241 gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga catgagagta
    51301 agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga gaaacatagc
    51361 agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt tattcatatt
    51421 agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt atggcattca
    51481 ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt attttacccg
    51541 gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt tcccattctg
    51601 tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt tcttttatta
    51661 ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg ttactagtga
    51721 tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg tttggtttcg
    51781 gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc gtagtttctt
    51841 tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa acgatggggg
    51901 cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc ggcttctttt
    51961 ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga gaatggctaa
    52021 gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac cagatcgata
    52081 aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat ggcgcttgtt
    52141 cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag gggatcaaat
    52201 gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg gcactgcaaa
    52261 agaaacaagt agcaaacaag aaataaagga tttgaaaaaa gtgacacttg ttttggatta
    52321 tgtaccgaat actaatcata ctggcatcta cctagcaaaa gaaaaaggct attataaaga
    52381 ggctggttta aatgtccaaa tcatcgaacc aggagataat tcgacaagta ttggtctagt
    52441 aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc
    52501 agatggacag aatataccag tcaaagcaat tgctacagtg atcaaacata atacttctgg
    52561 attcgccacc ctatcagaca gtaacatcca ttcaccaaaa gattttgaag gaaaaacata
    52621 tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg
    52681 cggggacttt tcaaaactga caatggtagg aagcaatgga gaagggccag aaagcttagg
    52741 aaaatcctca gatatccaat ggtactttga aggttgggat atgatcaaag caaaagaagc
    52801 gggtattgaa ctaaactata tcccattgaa agaattagat gaacggttag attactatac
    52861 tccggtaatt attacgaatg atcaactgat caaaagtgat ccagaattgg tacagtcttt
    52921 tatggatgcc acgaaaaaag gctatcagga agctattaag gatccaaatg acagtgctaa
    52981 actgttgcaa aaatatgcaa aagaaaatga tcggacgttt ttagaagaat cacaagcatt
    53041 cttgtcaaaa aattatacgg atgatcctga gaattgggga ttgatggaag aaaaagtttg
    53101 gcgcaattat acagctttta tgcaggaaaa cggattgatc aagcaagatg taccttcgca
    53161 agaactattc actaatcagt ttataaaata ggagtgatag atatgtcaat ccaaatcagc
    53221 cacttgaatg tagtcattga aaacctgaaa atattagaag acatccattt cactatccct
    53281 aaagatacct ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa
    53341 actttaacag gagtccagcc tatgacttct ggaacgatca aagtagacga tcaaaaagta
    53401 actggtctcc atacagcatt tagttatatg ccgcaagaag atatgttact tccttggctg
    53461 agcgtctatc aaaacgttac gttatatcaa aaaatcaatc atctaacaat caatgaagaa
    53521 caagtaaggg attatctgga tatctttggg ttaaacggct atgaacattt tttgccggaa
    53581 caattatctg gcgggatgaa gcagcgaacg gccttgttac gaacaatcat gaatccttct
    53641 tcttacttgc ttcttgatga accatttgga gcgttagatg ctatgaccag agggcagatg
    53701 caagactgga tgttgaagct gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat
    53761 gatatcgaag aagcaattta tttatcagac aggattcttg tgctttccgc ccgtccggca
    53821 catgtgattg ctgagattca agtgcctgaa aaacgacggt cacgtgaatg gctactgcag
    53881 caatcagagt tgaagcaaac gatttatcag ttattggcag gtgaattaga tgttaagtga
    53941 tgctcattgt catttagatg ggagtcagag tttagcactc cttcagcagg aacacacgat
    54001 tttgacgatc atcaattgtg attcgccaga agaatggaag gaaaacagac agcttgcagc
    54061 aagtaagact caagccttaa gttacggtat ccatccttgg aaagcagata gctatacgtt
    54121 tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa
    54181 tatttggacg aatgtaccga tgactacaca aaaaaaagta tttgagcgac agcttgcttt
    54241 tgcagccata aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt
    54301 aaattatatc caagcttacc ctaatcacta tttgatccat tggtattctt ctttggacta
    54361 tcaacaagac tatattgatt taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa
    54421 tccagcagtc tggcaactag ctaaagcagt cccaatcgac cacttactta ttgaaacgga
    54481 tggactacat gcagcagagt gggctttagg ggggattttt tcagaaaaag attatgtctc
    54541 actgcttgaa gaacagttaa agacaatcgc tgagatcaaa gagctatcag ttgaagaagt
    54601 taaaaaaaat acttatcaaa acttggaacg tttcattaga ttaggtaaat aaaaaagaga
    54661 tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt gtataaaagt aaactgtttg
    54721 attaataaat gaatcttagt accaaccgtt tgcttcccag aatgctttcg ccgcttccca
    54781 agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg cagcagagta
    54841 gtcaccattt aagtaagaag aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc
    54901 tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct tttgcagagc ttgtgcttgc
    54961 tggagtagca gcagtttctg ttacagcagg agcaggttgt gtttcagcaa caggcgctgc
    55021 ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt tcagcttgga ctggtgcttc
    55081 ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag ccttctgttg ggattgttaa
    55141 ttgttgtcct gagta
//
LOCUS       DACECB010000012        47991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECB010000012 DACECB010000000
VERSION     DACECB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.012"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(801..1142)
                     /locus_tag="IWO04_000782"
     CDS             complement(801..1142)
                     /locus_tag="IWO04_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0212412.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1142..1372)
                     /locus_tag="IWO04_000783"
     CDS             complement(1142..1372)
                     /locus_tag="IWO04_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0212413.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1659..1997
                     /locus_tag="IWO04_000784"
     CDS             1659..1997
                     /locus_tag="IWO04_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212414.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2202..2441)
                     /locus_tag="IWO04_000785"
     CDS             complement(2202..2441)
                     /locus_tag="IWO04_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0212415.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2417..2764)
                     /locus_tag="IWO04_000786"
     CDS             complement(2417..2764)
                     /locus_tag="IWO04_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0212416.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3496..4077)
                     /locus_tag="IWO04_000787"
     CDS             complement(3496..4077)
                     /locus_tag="IWO04_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0212417.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4260..4886)
                     /locus_tag="IWO04_000788"
     CDS             complement(4260..4886)
                     /locus_tag="IWO04_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0212418.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4908..6239)
                     /locus_tag="IWO04_000789"
     CDS             complement(4908..6239)
                     /locus_tag="IWO04_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0212419.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6394..6795)
                     /locus_tag="IWO04_000790"
     CDS             complement(6394..6795)
                     /locus_tag="IWO04_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0212420.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6881..7087)
                     /locus_tag="IWO04_000791"
     CDS             complement(6881..7087)
                     /locus_tag="IWO04_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212421.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7182..8684)
                     /locus_tag="IWO04_000792"
     CDS             complement(7182..8684)
                     /locus_tag="IWO04_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0212422.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8702..9157)
                     /gene="argR"
                     /locus_tag="IWO04_000793"
     CDS             complement(8702..9157)
                     /gene="argR"
                     /locus_tag="IWO04_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0212423.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9255..9872)
                     /locus_tag="IWO04_000794"
     CDS             complement(9255..9872)
                     /locus_tag="IWO04_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212424.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10093..11466
                     /locus_tag="IWO04_000795"
     CDS             10093..11466
                     /locus_tag="IWO04_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0212425.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11541..12653
                     /locus_tag="IWO04_000796"
     CDS             11541..12653
                     /locus_tag="IWO04_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0212426.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12725..14362)
                     /locus_tag="IWO04_000797"
     CDS             complement(12725..14362)
                     /locus_tag="IWO04_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0212427.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14530..15138)
                     /locus_tag="IWO04_000798"
     CDS             complement(14530..15138)
                     /locus_tag="IWO04_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0212428.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15522..16673)
                     /gene="queG"
                     /locus_tag="IWO04_000799"
     CDS             complement(15522..16673)
                     /gene="queG"
                     /locus_tag="IWO04_000799"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0212429.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16692..17444)
                     /locus_tag="IWO04_000800"
     CDS             complement(16692..17444)
                     /locus_tag="IWO04_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212430.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17585..18535)
                     /locus_tag="IWO04_000801"
     CDS             complement(17585..18535)
                     /locus_tag="IWO04_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212431.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18536..19375)
                     /locus_tag="IWO04_000802"
     CDS             complement(18536..19375)
                     /locus_tag="IWO04_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0212432.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19372..20118)
                     /locus_tag="IWO04_000803"
     CDS             complement(19372..20118)
                     /locus_tag="IWO04_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212433.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20356..21726
                     /locus_tag="IWO04_000804"
     CDS             20356..21726
                     /locus_tag="IWO04_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0212434.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21781..22677)
                     /locus_tag="IWO04_000805"
     CDS             complement(21781..22677)
                     /locus_tag="IWO04_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0212435.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22958..23635)
                     /locus_tag="IWO04_000806"
     CDS             complement(22958..23635)
                     /locus_tag="IWO04_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0212436.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23726..24631)
                     /locus_tag="IWO04_000807"
     CDS             complement(23726..24631)
                     /locus_tag="IWO04_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0212437.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24806..26272
                     /locus_tag="IWO04_000808"
     CDS             24806..26272
                     /locus_tag="IWO04_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0212438.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSIKKGS"
     gene            26275..27306
                     /locus_tag="IWO04_000809"
     CDS             26275..27306
                     /locus_tag="IWO04_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0212439.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27313..28500
                     /locus_tag="IWO04_000810"
     CDS             27313..28500
                     /locus_tag="IWO04_000810"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0212440.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28656..30038
                     /locus_tag="IWO04_000811"
     CDS             28656..30038
                     /locus_tag="IWO04_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0212441.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30010..30396
                     /locus_tag="IWO04_000812"
     CDS             30010..30396
                     /locus_tag="IWO04_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212442.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30596..31264)
                     /locus_tag="IWO04_000813"
     CDS             complement(30596..31264)
                     /locus_tag="IWO04_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0212443.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(31453..32250)
                     /locus_tag="IWO04_000814"
                     /pseudo
     CDS             complement(31453..32250)
                     /locus_tag="IWO04_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32531..33910)
                     /locus_tag="IWO04_000815"
     CDS             complement(32531..33910)
                     /locus_tag="IWO04_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0212444.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(34085..35254)
                     /locus_tag="IWO04_000816"
     CDS             complement(34085..35254)
                     /locus_tag="IWO04_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0212445.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(35229..36380)
                     /locus_tag="IWO04_000817"
     CDS             complement(35229..36380)
                     /locus_tag="IWO04_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0212446.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(36340..37701)
                     /locus_tag="IWO04_000818"
     CDS             complement(36340..37701)
                     /locus_tag="IWO04_000818"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0212447.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(37698..38699)
                     /locus_tag="IWO04_000819"
     CDS             complement(37698..38699)
                     /locus_tag="IWO04_000819"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0212448.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(38715..39410)
                     /gene="dapD"
                     /locus_tag="IWO04_000820"
     CDS             complement(38715..39410)
                     /gene="dapD"
                     /locus_tag="IWO04_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0212449.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(39416..40132)
                     /locus_tag="IWO04_000821"
     CDS             complement(39416..40132)
                     /locus_tag="IWO04_000821"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0212450.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(40129..41010)
                     /locus_tag="IWO04_000822"
     CDS             complement(40129..41010)
                     /locus_tag="IWO04_000822"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0212451.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(41013..41813)
                     /gene="dapF"
                     /locus_tag="IWO04_000823"
     CDS             complement(41013..41813)
                     /gene="dapF"
                     /locus_tag="IWO04_000823"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0212452.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(41800..43059)
                     /gene="lysA"
                     /locus_tag="IWO04_000824"
     CDS             complement(41800..43059)
                     /gene="lysA"
                     /locus_tag="IWO04_000824"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0212453.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(43545..44108)
                     /gene="efp"
                     /locus_tag="IWO04_000825"
     CDS             complement(43545..44108)
                     /gene="efp"
                     /locus_tag="IWO04_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0212454.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            44536..45453
                     /locus_tag="IWO04_000826"
     CDS             44536..45453
                     /locus_tag="IWO04_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0212455.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            45466..46611
                     /locus_tag="IWO04_000827"
     CDS             45466..46611
                     /locus_tag="IWO04_000827"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0212456.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            46782..47240
                     /locus_tag="IWO04_000828"
     CDS             46782..47240
                     /locus_tag="IWO04_000828"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0212457.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            47399..47869
                     /locus_tag="IWO04_000829"
     CDS             47399..47869
                     /locus_tag="IWO04_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0212458.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT        13573 a         9892 c         8599 g        15927 t
ORIGIN      
        1 ataatattga caaaaagtga ctcaaaataa aaaaaaactt acattatact ggaatgaact
       61 gtattgatag tgtacctaaa agctctactg acataagatc gtgtaatcgg taaagtaatt
      121 ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa ggtactttta
      181 cattcataat tgtcgctttg agattctact ttggttcact atctgaccca attgcacatt
      241 ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag cggtagattt
      301 ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt ctcttttcat
      361 tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat agaaattctt
      421 tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag tttaaaatga
      481 cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt tgttcttttt
      541 cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa
      601 acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat cattcgttta
      661 aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc acactagtag
      721 aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata taaaaatatt
      781 atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat tttagccatg
      841 tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc aagagatttt
      901 aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga gtaacgtgtc
      961 ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt tttacggtta
     1021 aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt tcctacggag
     1081 ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct ttcttctgac
     1141 actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc tcataatact
     1201 cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta attgttccat
     1261 catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg ttatcagaag
     1321 gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc attatcatca
     1381 ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc tttgtaaact
     1441 atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa ataaattgta
     1501 gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac ctatggatcc
     1561 aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca gataaaacgc
     1621 tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat aaacaaaaag
     1681 ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg
     1741 aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca ttggttcaac
     1801 gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct atttttgtag
     1861 aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa cctcttatct
     1921 ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt agattccaag
     1981 aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac tcgcagcacg
     2041 agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta tagatgcagt
     2101 atatgcagct tatgagtatt tcttctgttc tctttgtttt tgggatctgt gctgaagtct
     2161 ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt ctccattact
     2221 aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt tacccaaggt
     2281 tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc gactgtgcgg
     2341 ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg gctacgataa
     2401 aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc tttttcttca
     2461 ttccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     2521 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     2581 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     2641 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     2701 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     2761 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     2821 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     2881 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     2941 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     3001 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     3061 aaagctattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     3121 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     3181 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     3241 cctctccact aaacgatgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     3301 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     3361 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     3421 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     3481 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     3541 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     3601 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     3661 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     3721 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     3781 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     3841 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     3901 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     3961 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     4021 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     4081 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     4141 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     4201 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     4261 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     4321 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     4381 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     4441 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     4501 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     4561 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     4621 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     4681 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     4741 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     4801 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     4861 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     4921 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     4981 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     5041 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     5101 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     5161 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     5221 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     5281 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     5341 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     5401 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     5461 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     5521 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     5581 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     5641 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     5701 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     5761 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     5821 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     5881 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     5941 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     6001 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     6061 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     6121 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     6181 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     6241 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     6301 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     6361 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     6421 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     6481 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     6541 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     6601 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     6661 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     6721 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     6781 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     6841 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     6901 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     6961 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     7021 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     7081 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     7141 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     7201 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     7261 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     7321 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     7381 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     7441 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     7501 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     7561 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     7621 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     7681 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     7741 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     7801 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     7861 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     7921 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     7981 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     8041 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     8101 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     8161 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     8221 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     8281 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     8341 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     8401 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     8461 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     8521 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     8581 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     8641 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     8701 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     8761 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     8821 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     8881 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     8941 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     9001 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     9061 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     9121 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     9181 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     9241 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     9301 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     9361 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     9421 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactacttgt
     9481 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     9541 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     9601 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     9661 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     9721 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     9781 atgctaaaga atgatttttt aggaacctct agtatttgcg ggcttttttt catactatag
     9841 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     9901 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     9961 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
    10021 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
    10081 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
    10141 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
    10201 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
    10261 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
    10321 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
    10381 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
    10441 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
    10501 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
    10561 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
    10621 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
    10681 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
    10741 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
    10801 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
    10861 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
    10921 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
    10981 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
    11041 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
    11101 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
    11161 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
    11221 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
    11281 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
    11341 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
    11401 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    11461 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    11521 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    11581 tgaccctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    11641 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    11701 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    11761 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    11821 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    11881 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    11941 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    12001 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    12061 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    12121 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    12181 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    12241 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    12301 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    12361 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    12421 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    12481 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    12541 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggccta
    12601 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    12661 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    12721 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    12781 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    12841 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    12901 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    12961 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    13021 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    13081 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    13141 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    13201 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    13261 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    13321 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    13381 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    13441 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    13501 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    13561 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    13621 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    13681 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    13741 gcacaaccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    13801 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    13861 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    13921 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    13981 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    14041 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    14101 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    14161 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    14221 ctagttgata agctgtcttc ctatcaaaaa cttcatgaac attattaccg taaaatgtcg
    14281 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    14341 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    14401 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    14461 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    14521 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    14581 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    14641 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    14701 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    14761 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    14821 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    14881 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    14941 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    15001 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    15061 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    15121 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    15181 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    15241 ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata caaaaccata
    15301 aaagccttgt tgtataagca aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa
    15361 aacgcagatt ctgtaacatt cactgcaata atccataaaa aaaagcaaaa aaatttttga
    15421 tttttatcaa attctcttaa aaaaatgtaa aaaatacaaa aaaaccagta attgttacaa
    15481 caattactgg acaaaaactt tgaataatgt taactatttt tttattttaa ctttctccgc
    15541 aataatttct gggcatgttc caattcgttt ataacgactt cttctgtttc tttagctaat
    15601 atttcatcta atacatcaag ccatttttcc ggttcttttg ttcctagacg tccaatagcc
    15661 caagctgctg ttcctctgat gacagggcgg acatctgtta aacattctga aatctcaggc
    15721 aatgccgttc gatcacctag attagctaaa gcaatcaatg cgtttctctg caaaggtttc
    15781 ttcccacgcc atgaaccagc taaatgccca aattgctgtt tgaagtcttt attagacatc
    15841 gaaagcattg gctttaattt tggatatatt tcgtctactt ttggttccat ctcttcatga
    15901 aaatgaaaat cttttccttg attgtaaggg cagactaact ggcagatatc acatccataa
    15961 atgacatttc gcattttttt acgatattct tccggcatca tcccttttgt ctgagtttga
    16021 taagacaaac atcgctgggc attcattctt ccgtcaccaa gcaatgcttt tgtcggacaa
    16081 gcagtcacgc agcgcataca gtcaccgcaa ccaaattctc ctggtttatc aggctcaaat
    16141 acaatattag taacgatctc accaagatag acaaatgaac cgaactcttc agtgatcaac
    16201 aatccgtttc gtccaataaa tcctaaacca gctcgctgtg ctacagcgac atcgatcagt
    16261 tctccagtat ctacttgcgg agctagacgc cattcctctt ctttttgcca tttttctgct
    16321 cttgtttgga tgaattcgat caatcggttc aaacggtctc tgagtatatc atggtaatcg
    16381 atcccccaag aagcacgagc aaactgcccg cgtttttcat ccctaggcac ttcttcatgg
    16441 atcctagtag gatacgcaag tgcaatggaa atgatcgatt tcggctgatc aaatgtcatt
    16501 tctggataaa gacgctcatc gatatttgga tgttcaaagc cagaagtatg gccagctgct
    16561 ttttgttctt ccaacgattc ttttaatgaa tcaaaaggtt ccgccgtggt gaacccgatt
    16621 ttgtcaattc ctaatcgttt gctttcttgt atgatttctt cttttaatgt catggtcttc
    16681 ctcgcttgct gttaacggct gtataaccca taatatagtt tttctttttt cagctggaca
    16741 aactggatct gctgatcggc aatcttcttt tcttccatcc atttttttct tgcagcaaat
    16801 acttcccaat aatttgttga aattaatttt cttttgttat ctaatgcatt aaaaaaattc
    16861 aatgaaactg gttttgtact taacagttca tggagcctta cctcttccca tggcgaaaac
    16921 tcagacagat cgcgggaata caatacttct tcatagagat ttttttgctg tgtgtacgtg
    16981 aatgtttgcg ggattttccc aggcctagca atttctacta gacgattata aaacatattc
    17041 attaatttgc tgttggcaat aaattgactt ttataatagg ccgactggat ctttttgatt
    17101 actccttctg ctttgatgac tgaaaataat gcaggccatg ctttgacggt tatttcgtcg
    17161 ttttgtttaa ctaatacgcc aggtgtgaag ccgactggag aaaggaacac aaaataaccg
    17221 ataccggtat tttcatctaa aggctgaaat tcatcaaaca accatttgcc tatgtacagc
    17281 tcttcatctg gttctgttgt ctcattttga tttgctatga ataatttagc taattctgct
    17341 tcaagaaatt gaaggtcttg atccttgtcg gtattttcct gctggctgat caatcgaaag
    17401 acttcttctt taatcgtata tttatcttta cgtgtaaaat tcatagccac accttccttc
    17461 cttactctaa ctataacgga atttcttcaa aaaaggtatc tgtacgacga aaaacagcat
    17521 aatttttttt catatctctt ataaaaaatg caaggtctgc tcctcaagct agaccttgca
    17581 cttttcagct ttcttttgtt tgtgacaaac cttcatggat cgtttcaata ttccatttca
    17641 tcatctgata gtaagaatct ccttgttctc cttcttttgc tatggaatca gtgaacagtt
    17701 tatcatagat tttcaaaccc gtttcttttg ctacccgttc catgcttctt gagtctacac
    17761 tggtttccac gaataaaacc ggtacttctt ttgctcttat ctgatcaatg atcgctttca
    17821 tttgatcagg cgtaccttga ctttctgtat tgatctccca tatataagct gctggcaaat
    17881 catatgctcg tgaaaaatac ttgaatgccc cttcacttgt tactaataat tttttgttct
    17941 cttcgataga agcaaaactt tccttcgctt gtgtatctaa ttcttttagt ttttccacat
    18001 atgcttctgc atttttttta tagagtgtcg cattttctgc atctttttca gagaatatac
    18061 gtgcgatttc ctccacatat tttattccgt tagataggtc tagccaagca tggggatctg
    18121 cttttgtatg ttcttcaccg ctagttaaat aaagaggttc tacatttttg ctaactgcaa
    18181 aataatcttt tccttcttct tttttagccg tttccatcaa gttatcgaac cagctgttac
    18241 ctgtttcaag attcaaaccg ttgtataaaa taacatctgc atcgcttgcc tttttgatgt
    18301 cttctggtag tacttcatat tcatgcggat ctgttccgac tggtacgata ctgtggatat
    18361 ctatttgatc tgttccgacc tcttttgcca tgtccgccaa gatcgaattg gtagccacga
    18421 ctgacaactt ttctttactt ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg
    18481 ctaataccaa gaaactaact gccaagatta aaaagagtga tttttttgtt ttcatctatc
    18541 tgcttccttt ctgaaataaa attccttttg ttggagaaaa taaaaaagca aggataaaaa
    18601 atgcggtaca tgctaacacg atcgtagctc ctgatggcca attgtaacta taactgaaaa
    18661 acacaccgac aactgcactt agcataccga ttcctgcagc tgtaatgatc attttcagta
    18721 aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat caacatggca atcaccaaga
    18781 ttgttcctac tgtctgtaaa ctgactacag ccactaatgt caaaaagaac atcaaaagat
    18841 aatggatcag cgatgtgttc agcccataag cttttgccat tgtcggatcg aatgatgtga
    18901 tcttcaattc tttataaaag aaaaagacaa atatcaagac gattccacta acgagtgctg
    18961 tcagcaacaa atcgctttcc ctaacagcta agacattccc aaataaaata tgatacaaat
    19021 ctgtcgcact ctgggcatat gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga
    19081 caattccaat cgctgtatca tttttcaatc ggctccgctg agtcacgaaa ccaatcactg
    19141 ctgctgttag catcccaaaa gcagtggctc caaagatgta actaaaacca aacatataag
    19201 aaatggctac acctggaaga actgcgtgag agatagcgtc tcccatcaaa gacatccctc
    19261 gtaaaataat aaaagaaccg atgactccag aagcaagtcc tacaagcatg gaagtgacca
    19321 aagcgtattg caggaattga taattcatta atccatcaat aaaggattgg atcatttttt
    19381 ctctcctcct tttactaaat gtctgatgat ctcaccatag gccaattgaa ggttcttcga
    19441 tgtaaagact tcttgtactg gaccggaagc gatcagctgt ttatttaaaa gaataacctc
    19501 atcgaaatat tcttctactt tatgaagatc atgatggacg atcaaaatcg tttttcctga
    19561 attcttcaat tcctgcaaga tgtcaaagat ctttcgttca cttaacgcat caatccctac
    19621 gaatggttca tctaaaaaga tccattctgc tccttgggct agagctcttg caataaaaac
    19681 tctctgaagc tgtccacccg atagttcgct gatctgtctt ttggcatatt cttccatccc
    19741 tacttttttc aaagcttgtc ttgcacgttc tttttctttt ttcccaggtc tttgtccaat
    19801 tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat acatcaattg gaaaggaaag
    19861 atccaattca cttcgctgtt ccacgtaggc tattttttta cggactgagt ttaccgactg
    19921 tccagaaaag gtgactttac cagtagtagc tggtatcaac tctagcaaag atttcataaa
    19981 tgttgatttt cctgcaccgt ttggtccgat aataccggta attttttgtg gatttatcgt
    20041 caagttgata gttttcaaaa cagttttccc ttgataagcg gctgaaaggt cttgaattac
    20101 aatggccgta cgttccaaat cttacactcc tttttaggtt tacctaactt tttatttaaa
    20161 tatagtttaa ctaaaatgga aagcattttc aagtagaatg atgaaaaagt ttttttcgaa
    20221 ttttcctcgc attttatgaa aattttctgt agaatcacat caagagttca ataaggctaa
    20281 cctaataatg aaaaacatgt gttgaaacgt ttgattattt tttcttgttg actcaacaac
    20341 gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc
    20401 caaatgatcg cactaggtgg aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc
    20461 atacgttgga cagggccctc agtgatggga gcctatgcga ttgcaggatt ctttttgtat
    20521 ttgattatgc gtgctttagg cgaaatgctt tacgttgatc cttcgactgg ttcttttgca
    20581 aattacgcga gcgagtacat ccatcctgtg gctggctact tgaccgcttg gagtaatatt
    20641 tttcaatata ttgtcgtagg aataagtgaa gtgattgctg taggatctta tatgaattat
    20701 tggtggcctg atttgccggc aattattccc ggaatcattg tcgtcttgtt tcttatgctg
    20761 gcaaatctta tttctgtcaa ggcgtttggc gaattagaat tttggttttc gatcatcaaa
    20821 gtcataacga tcattttgat gatcattgca ggtcttgggg taatcttatt tggatttggt
    20881 aatcatggtc aggcagtagg gatttcaaat ctttggaaaa atggtggatt cttcactgga
    20941 ggcgtcaaag gatttttctt tgcattatcg atcgttgttg cttcctatca aggaatcgaa
    21001 ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa agacgatcat tgaagctgtc
    21061 cagtccacta tcggtcgaat cttgattttt tatattggtg ctatttttat cattgtgagt
    21121 atctatccgt ggaatcaatt gagtgagatt ggttcacctt ttgtacaaac tttttctaaa
    21181 gtcggcatta catttgctgc aggactgatt aattttgtcg tgattacagc ggcattatca
    21241 ggttgcaatt cagggatttt cagcgcaagt cgtatggtct atacacttgc cataaatgga
    21301 aagatgtcta aaaaatttct gaaactatct cgtcacggtg cgccttttta tcctgtcgta
    21361 gcaatctcag gtggaatctt aattggtctg atcttaaatt atcttctacc aatgttcatc
    21421 aaccagtcag aaaatatttt cgttttcgta tacagctcaa gtattttgcc tggtatggtt
    21481 ccttgggtag tgattttgat cagcgagatt cgcttcaaaa agaagcacgc agataaaatg
    21541 catgatcatc cttttaaaat gccactatct ccttatacga attatgtgac acttgctttt
    21601 ttagctatcg tattgatttt catgtttatc aatcctgaaa cgagaatctc attgattatt
    21661 ggactcgttt tcttgcttta catgacgatc cattactttg tacgcgaaaa aagaaccgga
    21721 aaataaaaaa agaacgaagc ttcaccttag tggaagcttc gttctttttt attgctttta
    21781 tcaattcaat tttagcgtct caccgggagt caggaaataa ttctccatgc tcatcccatt
    21841 cagcgacaag atcgtttcta ctggtacgcc agttcgttcg gctatttgat ttggaccttc
    21901 tccttccaaa actacggttg tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga
    21961 ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca gaagcttcta ccgactctgc
    22021 tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc
    22081 gctagtctgc aaataaatat cggaaatctt agcgacatga ttcgtataat tagccgataa
    22141 ttgattgatc aaagtatcta agtctacacc gtctttattt tctgctgcct gatacgctgc
    22201 acgtaatttt ctgatccctt cgtcggagat tgcttgaagc tcacgtgctt tttggttagc
    22261 aactgctgat aaaccagcta ttccctcttg atttccctga atttctgtct ggtattcttc
    22321 aatcaatgca ggcgtctttt cttcgatctt tttcgaataa tcatttacga ttgcttgaag
    22381 ttgctctggc aaaccatttg ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt
    22441 tgatgactca cttgtttgtt tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt
    22501 acttttcgct tctgacgaat tttttccatg aaaatataga cccgcaactc ctcctgctat
    22561 gatcaaaaac acggcaactg ctgccccaat catgatcatc cgcttacgag ctgcttgttt
    22621 ttgctgtctt cttcgggtca tccgaacatt ttctccccac tcattttcat catacatttt
    22681 tttcaccacc aatttttatg tataatagcg ttatcaaacg cttcatattc ttattgtagc
    22741 aaataattgt cgctgtgcac atttttatct ttgttattta taagaaaatt tcaccagttg
    22801 agatgattcc aagattgtcc gtcgatttct ccttgcagct gcattgttcg ttcgtctatc
    22861 tcagccatca gtttctttat ttcatataag agcgcacggt cttgatattc tgatttttcg
    22921 cttaattcat ctaattgttg gatcagccaa tcatttgtca taatgaagtt cctttcattt
    22981 cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc ctcagtcagt gcttctacga
    23041 tatatgcttg tttttcagat tcttcttcct gcattttctg attttcctgt actctttgaa
    23101 taaaccagtc aatccgctga gtcagcgatg gtttgccttc caatgagaga tactgctgat
    23161 aagcaattag cttctcccaa cgtttttcct gggataaata cataaattgt gctaaaacga
    23221 atggcggaag ataggtcata tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt
    23281 cggaaatcgt atattcttct tttcctccag cttggtattc tttttccgcc acacctgttg
    23341 tcaatacgat tcctagttct ttccctttta atacatgtgc tgctcgttcc aagacattat
    23401 cttgccaaat ttttaaatga gcaggagaac tataccagta aaacggaaac tggaaaatga
    23461 tccgatcatg ctgggccaag agctgtagtt cagtctctat atctattttt ccgtctggat
    23521 aacttgattc caaatgatgc caagtagctg gtaaaccagc agaagcttct ttgaaaaatt
    23581 gctgatttaa agattcttcg atcgttggat ggctaatgat gataagtgta cgcatccctt
    23641 ttcctccttt ttagtcttcg ctttacagtg tagcataacc gccacaagtt tcttttgccg
    23701 atgcttgtca ttttaaccag actaatcagg ttgtccaaga aactgcggtt tgtttttcca
    23761 actgtttttt caaggtatcc aaagcaatat tcttaaaatt ttcgtttttc atacgtttac
    23821 gatagttcgt agccgtttct ccggtactct ttttgaacat tcggctaaat acgactagac
    23881 tattgaaccc tacttgatag gcaatacttt ccacggattc atccgtcaga gaaagaagaa
    23941 acgcccctcg attgatccgt accccgatca aatattcctt gatactggta cccatatgat
    24001 ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa atgctgggcg atttcctcaa
    24061 cagacaattt ttcagcataa tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg
    24121 aatgaacctt ttgtacacca ccgctgacct ttccgccatc ttttaaaagg actgataaaa
    24181 aacggtaggt taatgctgtt aactgtaatt cattttccaa ggtttcttcc gtatctttca
    24241 tgcattctaa aataatgtcg atatattttt gtatgtcaga agagaccatc gtgtattggt
    24301 cttctctgaa aagcgactgc cgaatgattt cttctgcagc actcccacca aatgaaagcc
    24361 agcaatacat ccacggatct tctccatctg ataagtaaaa cgtcgagtct cctggacgaa
    24421 tcaagaacag atcacctttt ttcaattgga attgttgatt tttcacatga taagatcctt
    24481 tgccttccat cacgacatgc aggatgtaat cattgcgaat agcaggacca aaagaatgaa
    24541 acggcatcgt tttagagaaa ccacaaaaat caaaatacaa ttctttttct acttgttgat
    24601 gttccttttt taaaaaaatc gattgctcca cgtctatccc tccttactcc tttagtataa
    24661 tgcaacaaaa agttaaataa aagtcacaga aatatattaa aaaggaagcg atttcttgtt
    24721 aagtttaagt cattaaaaga tctttgttat tcgttgtaac cggtttactt agtattatat
    24781 attgaacagg agaggaatac agattatgaa aagcaaaatg atccaacgtg tgtcctacgc
    24841 tttaggcgct tttggacacg acacattcta tgttacatta agcacttatt ttatggtatt
    24901 cgtgacaagc cagatgttcg caggaacaga taaggcaacg aatgcaaaaa tgattgctac
    24961 agttacaagc ttagttgtag gtatccgctt agttgaaatt atttttgatc ctttgattgg
    25021 tggcatcatc gataatactc gtacaaaata cggtaaattc aaaccatggt tagttatcgg
    25081 cggcgttgtt agttccgtac tcttggtagc aatctttacc aatttctttg gcttagctac
    25141 tcataatacg actttattta tcatattatt tgcaatcgtt ttcatcttat tggatagttt
    25201 ttattcattt aaggatatcg ctttttggtc aatgattcca gctttgagta cggaaacaaa
    25261 ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct tctctaggtg caaatggtac
    25321 tactctagtc gttgtcccta tcgttacttt ctttacctat cttgttactc ataaacatat
    25381 tgaaggcgct tcaggctggt tctggttcgg tgtgattgct gctgttattt caggcggtac
    25441 tgcgattatc actgccttgg gcacaaaaga agtcgatact gcgatccgtc agcaaaatga
    25501 aaaagctggg atcaaagatg tgttcaaagc agttgctcaa aatgatcagt tgatgtggct
    25561 tgcccttgct tatatcgctt atgcgattgc caatgtagca acaactgccg tattattcta
    25621 cttcttcaaa tatgtacttg gccaaccaac tgaattttgg atcgttggtc tagttgctgc
    25681 cttacttggt ttagtagctg ttccactatt cccaatgctt acacgcgtca tctctcgtcg
    25741 ctacgtttac cttggaggga tcgcaatgat gatttcttct tactttttct ttacgatcgc
    25801 aggttcaagt ttgccaatcg ttatcatcgg gctagtattg ttctacttcc ctcaacaatt
    25861 gattttctta tccgctttga tgacgatcac agactctgtt gaatatggtc aatggaaaaa
    25921 tggtatccgt aacgaagctg ttactctatc aattcgccct ttactagata aaatcgctgg
    25981 tgctttgtct aacgggatcg tagggtttgt cgctatcgct tctgggatga ctggtaatgc
    26041 tactgctgct gatatcactg ctcatggcgt aacgactttc aaagcttatg cattctatgc
    26101 tcctgctgct ttgatgattt tctccgctat tatttttgct tggaagatta cattgacaga
    26161 gaaaaaacat gcacaaatcg ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat
    26221 ggaagatgaa cttcacgatg cagtagttcc ttccattaag aaaggatctt gaaaatgaag
    26281 attacagaaa aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag
    26341 ctagcacttt ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga
    26401 gaattagttt tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt
    26461 gcttctatcg gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa
    26521 acttaccaat tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga
    26581 tttgaattaa aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt
    26641 tttactacta caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc
    26701 cgctatactt tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa
    26761 gatactttat tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag
    26821 tcaatcgatc aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat
    26881 agtattccta taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct
    26941 aaaaaattag cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc
    27001 gatcatccat ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc
    27061 cccgataaaa gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact
    27121 gcaaatttcg gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc
    27181 atcacatttg agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt
    27241 catttaaaag ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag
    27301 gaataactca tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga
    27361 cctaaaaaaa ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca
    27421 gattacaatg gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca
    27481 gcacctcgag acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc
    27541 attagtttta ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta
    27601 aaaggaatgg tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta
    27661 ttagtggaag gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta
    27721 ttagtaggtg ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc
    27781 caaacaggta aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag
    27841 tttgccatcg gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa
    27901 tacgaaatgg ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag
    27961 cggcgggaat tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg
    28021 gcacgtttgc aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt
    28081 gaatcaacta ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt
    28141 tctgaaaatc agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca
    28201 tttggtcaac tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg
    28261 atcgaattag atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga
    28321 atgacaggag ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct
    28381 gccttcaaaa acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt
    28441 tatgttgcac acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga
    28501 taaaaaaatc aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc
    28561 caaatgtgag ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga
    28621 acgtgtgttc taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat
    28681 ttgtttatga aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg
    28741 cctccgtaga gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt
    28801 cctatccgtc cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg
    28861 cagcaaaaaa agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct
    28921 atccaagtga tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag
    28981 aaatcaattc tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg
    29041 atgaaagctt ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg
    29101 acatggctaa actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag
    29161 gaattggcga taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa
    29221 agcaaatgtt tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag
    29281 atatcactga cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta
    29341 tacattctgt ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg
    29401 tcatcggtac tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag
    29461 aatatacacc aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa
    29521 gacgagaaga aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga
    29581 gacgatctgc agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc
    29641 ttgatccaaa tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta
    29701 gcagagatct agcagatgct ttattgacca ttttccacca gcattaccac taccaggata
    29761 ttcggcgtgt aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc
    29821 tttttgaaga tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga
    29881 ttagaaaaaa atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac
    29941 gcgccatcgc acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag
    30001 gtggaaaaga tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg
    30061 ccatttggat taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa
    30121 aaaaatgaag aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa
    30181 atcgatagta tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat
    30241 ttaaaagatg aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc
    30301 tatgaagatt actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt
    30361 gagttaaagg aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac
    30421 ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc
    30481 ttatttttcg aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa
    30541 agtcgcttag ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc
    30601 catctattcg gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt
    30661 cttctgacca ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt
    30721 tctccataag ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc
    30781 cttcatctac aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca
    30841 tttgaccggc attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct
    30901 gtcttaagat tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt
    30961 tgatgacgcc gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt
    31021 catttttttg gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa
    31081 ataggacatg ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta
    31141 aaacaagcgt atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca
    31201 aacgtaccac taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa
    31261 tcattttttc tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg
    31321 catggaatat acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag
    31381 tgacccccaa aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct
    31441 aaaatgatat ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca
    31501 gtttaatttc tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga
    31561 ttttaattcc tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc
    31621 aaaaaaattc tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa
    31681 gattttgtac tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc
    31741 tgagtgaaac gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc
    31801 ttccattacc gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt
    31861 gaacatatcc ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc
    31921 cacttcatgg tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt
    31981 gtagaaacga cgatggatcc gatttttggc tactgctcca acttttcctc gataagaatt
    32041 gtagcgacgt gatttacgcg tgtatgccgt tacttttatt cctagcttct tgatcaagcg
    32101 ctgaactttc tttttattga tagagaaacc gcggtttctt agttcattgg tcatccgtaa
    32161 acagccataa tccttgtgct tctcccgaat tttaagcatt tccgcttcaa tctcttgatc
    32221 tggattttta cggtccaatc gcctttgcca tccaaataat catgaacgat ctttaattta
    32281 agttcaaaag tatatttaac cataaaaaat accgacctcc aaaagttaga ttttttggtc
    32341 taacttttgg gggtcggtac acaaatcgtg tcacagtccc ttttttgaaa ataaacggtg
    32401 ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctgttt
    32461 ttacttggct gttcttatta cttgatgcaa ggcagctttt tcacaacctc ttttcaaata
    32521 tttcgttttc ttattctaca aatggttctt ctcctaacgc ttgttgagcg gctagattca
    32581 acatgctcca ttgtcggtca aagcctggct ggaagaagaa gtcagcttct gctagttctt
    32641 ctaaagtcag tttgttttgg atcgctagtg ccaatacatt tccttgacct gtaacatcat
    32701 aggtagataa tactgctcct cctagcaatt gatgggtcat tggatccgag aataattgga
    32761 tggacacttt tggatttcct tgttcttctg gtacatattt tggtcgtaac gtatcttcat
    32821 aataagacac tcgtacatct actcctgctt ttgctgctgt aaagctattc aatccgcttg
    32881 ctgcaaaatg atagtcaaag acacttaatg cagaggaacc tacgactccg ccgaacggtt
    32941 tgtttggtac ttcttcaaaa agatgtttta ctacataacg tgcttctcta cgtgcaacag
    33001 tcgctaaagc gatcggcatt tttttgcgtg ctgggataga ataagcaagt gtagcatctc
    33061 ctactgcata cacatctgga agattcgttt gcaagtattc atttgtttgg atccagccac
    33121 gttcatctaa gtcgacgatc ccttttaacc attctgtatt tgccttcaca ccggcagctt
    33181 gaatgactag atctgctggg atttcttttt cacttgtttt gattgcctca acttttccgt
    33241 taccagtaaa agcttcgatt tttgcatttg tcaaaacttc aataccgttt tcatttaggt
    33301 gttgttctaa aatatctgtc atctcagcat ctaaataagt gcctaaaggt ctatcgatca
    33361 catcaagcaa tacgacttct ttcccagctt ttcggcttgc ttctgctgct tcgatcccaa
    33421 tgtaacccgc acctacgaca gcgatttttt taatagccgg atcatttaat ttttctttga
    33481 ttttggttgc ccaattgtat ccacgcatca agtagacatt ttctagatct gctcctggta
    33541 ctggcagatt gtttggtttg acaccagaac tcaaaatcaa tttgtcataa ggatattctt
    33601 ttgtttcgtt atctgatgcg ctagtaaccg ttactgtttt tttatcggca tcgatcgttt
    33661 ttacttcgtg attatttaaa atatgaacat tggattgcgg aaagttttct ggtctgaaat
    33721 tacgtacatc atttacatct gttaccttgt cttctaaata cagctccata ccacatgaca
    33781 tgaaggaaat atagtctcct gcttcaaata gcgtgatatc tacattttca ccgtatcgtg
    33841 tcaatagttc caagatcgat tgatgccctc catgagatgc acctacaatg acaactttga
    33901 ttttttccat tgatgaaacc tccttgaata ttcttttaaa atcattctaa acaatttaaa
    33961 cttaacatta cattagttat gaatcaaaag aaacgcgttc atttgaacta aatttaaaaa
    34021 acaatcgtct ccttgcagaa tataccgcaa aattaacgat tgtttttttg ataaaatcta
    34081 tgaatcactg ttctgttaaa aagtctttga tccgtttgca ggcctcatgt aatttttcca
    34141 tgctgctagc ataagaaatc cggatataac cttctccttc tgggccaaat gcaattcctg
    34201 ggatacacgc aacttttgcc tgttccgcta attctacaca aaactgccaa gagtcagttt
    34261 gaatggcagc tgggagtttg caaaacaagt aaaatgcacc gtttggctgg gtcacttcaa
    34321 atcctaaagg cgccaattgc tggaccagat agtctcttcg tgtttgatat tgctctttca
    34381 tattctcgct atctttcacc ccatttatta gcgcttcagc cgcagctttt tgcgacatcg
    34441 ttgtagcggc agtcaccaga tattgatgga ctttaattag ttcgtcaata agggcttttt
    34501 gagcaaaaat aaagcccact cgccaaccag tcatcgcatg tgacttggat aacccgctta
    34561 tcacgattgt ttgttctgga agataagtag ctatggacat atgcgttcca ctataagtca
    34621 attcagcata tacttcatca cttataacaa ataccggata tttttttaaa acctcagcga
    34681 ttgctttcaa ttgttttgca gacaatgtcg tcccagtagg attgttagga tagtttaaaa
    34741 tgactgcttt gacagctgat ccatattgtt caaatgctcg ttcaaattgt tccggctgac
    34801 agacaaaccc tgtgtcagaa gtatctatcg ggatcaattc agcaccagct agttccacta
    34861 atggctgata tcctgaataa gcaggcgctg gaataagaac cttgtctcct tcttccaaaa
    34921 cagataacaa cgcagaggca atcgcttccg ttgcaccaac tgtcgtcaaa acttccgttt
    34981 gcggatcata agttaatcca taacgctgtt gctgaaatat acaagccgct tcccgcagtt
    35041 cctctaatcc tctcattccg gtatagtgag aaaagtcttc ttcaatcgct gctattcctg
    35101 cttgcttgac atgttcagga gttggaaaat ccggttctcc cagggtgagt ttgatcacat
    35161 ctggtatcga cgatatttgc tgatcgaatt gtcgtatttt tgatacttct attctagaaa
    35221 gcctaggact aaatcgattg gataaattca tcattattta ctcgctcctc ttttgaactg
    35281 ctatcgattt ttacaagaag attttctgta aaaagaatcg ttttgtacca ttctacacca
    35341 ttttctaaaa catccatatc aaaatcgaac gtcggatcat gtaaagcagg ggtatcaccg
    35401 acacctaaaa agaaaaatac acatggatat tgttgcgtat atatgccaaa atcttcggct
    35461 tgaagaactg gagcatctaa ttcatgtagt ttgtttgaat ctaacagtgc tgcatataag
    35521 cctctatggt tgattacagc tggatagccg tcattatagc gaaaagaaat agtagctgta
    35581 gagtgaaact gttctgctaa atggtctaat cccctcttca aataattttg catatctgaa
    35641 ctgtaagaac ggatacttcc ggatacaata acttctccag ctaaaacatt tcggatcgtt
    35701 ccaccttgga tttggccaaa tttcattaga tgattccact ctgccggtaa ttgccggtca
    35761 aaatcataaa cattcttcaa aaatcgtaca gctgcctcca gactatcgat tcctttttca
    35821 ctagaagcaa tatgtgcaga ctggccttta atgataatgt ctgtttcact gctttgtgcc
    35881 atcaaaggtc ctggtcgaga gaaaacttct cccttagcta gtccaggcca tagatgaaag
    35941 ccaaatattg cttgtacttc atatttatcc aacaatcctg actgaataac gctttgtgcg
    36001 ccaccggaaa tttcttctga tggctgatag atcagtacta cattataaat aagatcctca
    36061 atgtgtttag ttataaagtc agtcaaccct aaaagcatcg ccatatgtcc gtcatgacca
    36121 catgcatgca tataaccttc atgcttagaa gcaaaagaga gattcgtttc ttctttgact
    36181 ggcagtgcat cgatatctgt tcgaaaagca atcgtttctt cttgctgatt atcaaaataa
    36241 aggagtagtc ctgtttcgcc gatttcatgg ataacggcct cgctgttttt tacttgttca
    36301 tataaatatt tttttgtctt gaattcatga aacccgattt caggaatttg atggagtgcc
    36361 attcgatatt ctttcagcat ccgtattcac catcccttca tagataccct tcactactgt
    36421 ttcatattga ttattcggta caccgatgat cagattcaac tcctgtgctc cttgtgataa
    36481 gatcgatgtg cgaatttcta atttgttcaa tatcgataac actttcccag ataatccaat
    36541 caattcttta tgcggacccc caacgacaga gatcaaagct aagtcttcga tcacgacgat
    36601 ttcttccacg ccaagtgttt cttttaattc tttcgtgatg agaaataact tatcggcaat
    36661 cgcttctgct gagacgatca ctccgatatt gtcgatccct gaaggaatat gttcgattga
    36721 tatctcatgt ttagcaaaaa tgctcattgc tttccagata aacccgattt catcggacat
    36781 atgacgtttg aataaagtga tggaaagaaa atccttgcgt ccagctattc ctgtcagtcc
    36841 attttccttt tctatttcgt gattcgatat ttttgtccct tgagcgaaag gatcattggt
    36901 attttttatt tggatgggga tatcagcctc ttgaactggg aaaacagctg cttcatgaag
    36961 aacgttagcc cccatataag ccatctctct cagttctcta aaagagattt cctcgatgat
    37021 tttaggatga tcgatgattc gcggatcagc catcatgatt cctgaaacat ccgtccaatt
    37081 ctcataactt tccgcttgca acatcttcgc cagtatcgcg ccggtaatgt cgcttccccc
    37141 acggcccaat aatttttctt ttcctgcagg gttcgccccg taaaaccctg gaacaaccat
    37201 tttcggatat cttttaaatg cttttgtgat ttcctgtgct gttttttcct gatcgatttt
    37261 cccatcatag tcaaagaaaa tcaggtcttt tgcgtcaata aactggtaac caagatactc
    37321 agccatcagt tgagcagtca gatattcacc tctacttact aaataatctt cagttatctt
    37381 ttcgtcttcc agttgtttct gtatgcgtgc tagttcatct tcaataggta actgcagggc
    37441 aagaacatct cttacttgaa tgaaacgatc acaaatcctt ttccaaaggg gtgtccaatc
    37501 aacatgatgc tgaaggtaag cataaatcaa ataaagtaag tctgttactt tgtcatcatc
    37561 tgacgtacgt ttgcctaaag cagaaacaac gatcacttgg cgattgggat ctgaaagaac
    37621 aattgtacgg acttttttga attgctcttc atgagacatc gatgttccgc cgaatttcgc
    37681 tactttcagc gtattactca tcttgccacc tccgctattt tctgcattaa agctgcagca
    37741 atctgtacgg cattcgcagc ggctcctttg cgtatgttat cggcagtcgt atagaacaac
    37801 aatccgttct ctaaagaagt gtcttttctg atcctcccaa cataaaccat atctgttcct
    37861 ctagcaagaa tgctcgtcgg atatttatgt gctgctagat catcttctaa ttgaatccct
    37921 ggaaactggc tgagacactc tctaatttct tccgtcgtaa actctttttg cagttgaact
    37981 gccacagaga ctccatgtcc attttcgatt ggcacacgta cgcaggttgc tgatactggc
    38041 aaagcaggct gatgcagaat cttacgggtc tcatttatca ttttctgttc ttcttttgta
    38101 tacccatttt ccaaaggctg atcgatttcc ggaatcaccg tttggctaat attataagga
    38161 tacaaaactg gttcctcgtc attttgcgta cgatgaagat cttcaatgcc tttttgcccg
    38221 gaaccagaaa ctgcttgata ggtcgaataa ttgactcgct ggataccaaa ggcttcttgt
    38281 aaagctttaa gcggaagtac cgcttgaatg gttgaacaat ttggatttgc aatcagccga
    38341 ttcaatgtat agtcttccag attgacctct ggaacgatta atggaatatc cgaatcttcc
    38401 cgccatgctg aggaattatc tatgacaaat agtccctgac ttcgtgcgat tggcgcaaac
    38461 tctttggata cagaagcacc tgcagaaaat agcgctaaat catatccttc aaatgaagtt
    38521 tctgtcaatt cctgtacagt gtgtttcttt ttcttgaata cacaaacttt accggctgaa
    38581 cgttttgaag ctaataattt caatcgtcct actggaaaat catattcttc taacactttg
    38641 agcatcgttt ggccgactaa tccggttgcc cccacaatac aggtatctat ttcgcgcatt
    38701 tttctccatc tcctctataa ttcgcgtaac gcagcaatca atgctgtatt ttcttttgtc
    38761 tgatcatcac ttatttttaa tacacgtgca ggtattccac cgacaacagt ttctgctgcc
    38821 acatcttcta aaaccactgc tcctgctgcc acgactgcat cttttccgat atggacacct
    38881 tcaacaatca ctgcattcgc acctaccagt acgccgtctt ctacgataac cggtttggcc
    38941 gatgctggtt cgatcacacc tgctaagaca gctcctgcac caatatggca gtttttgccc
    39001 actgtcgctc gtccacctaa gacagcaccc atatcaatca tcgtgttttc accgattact
    39061 gcaccaatat tgataatcgc tcccatcatg atcacggcat tgttgccgat tgacacttga
    39121 tctctgataa tagcacctgg ttcgatacga gcattaattt ttttaatatc tagcaaagga
    39181 actgcgctgt atcttgcagc agtttcgatt tcgatttcaa tgattttttc tgattcagct
    39241 tctaaaaccg gagcaatcgt ctcatagtca ccaaaaacaa cgaagctgct tccttcacca
    39301 aaaactttac tttctttaaa ttcgattggc tcggttacat tcagatagac tttcacaggc
    39361 gtctttttag atgccgtttg aatgtagcgg atgatttctt gagcttccac gttttttaca
    39421 cccctttgtc cattaataaa tcttctaatt tgtaataacc tggtgactga tctttcaacc
    39481 agtccgctgc ttctactgcg cctgcagcaa agattcgatt agaaaaagat tcatgtttga
    39541 tcgtaaatat ttcatcagta gcagcaaaca atacatcatg ttcaccaggt aaagaacctg
    39601 cacgaatact gtgtacacca atttggtttt cttctctttt tttcccttgc cagttgtata
    39661 cgggctgcag cttttgcact tcttggattg tttgaagcag catatttgca gtaccagatg
    39721 gtgcatcttt tttacgacta tgatgttttt caatcagttc gatctgccag ccttgcaaca
    39781 ttgcagctgc ttgcttgatt agttgattca tcaccatgat gccgaacgaa aaattagcac
    39841 tatacatcac aggtacatgt ttggcatgtt cttttatttc ttggaactgt gattgtgtat
    39901 aacctgtcgt gccaatgact aacggaagtt gatatgataa gctatacgaa aggattttct
    39961 ctaaattatc tggatgagaa aaatcaataa gcagttctgg cagttccgta aaagcaagcg
    40021 tagttgcttc tttaggtgcc gtccgtaaaa gaaggaccgt atctcctctt tcttgtgcaa
    40081 cttcttcgac tctatttccc atctgtccac tgccaatcag tccgattttc atttttcaac
    40141 cgtcctttct tttaacaaaa gctgagctaa ttgtttcttt ttctcttttg aaagagaaac
    40201 taaaggcagg cgtagaggtc cggaaggtaa gtctaataat gacatggctg ctttgacagg
    40261 aataggattg gtctcactga acaaggcatg gatcagttca agtgcattta attgcttttc
    40321 ttttgcttgc tgccatcttc cgtcctgcgt atcttttact aattcatgga ctattttagg
    40381 ttggatattc gcccatacac ttattacacc gcttcctcct acactcatta ctggtaggat
    40441 caaatcatca tttccactgt aaaggaagaa ttcttctcca atcaaccgag cggcatccat
    40501 cacataagcc atatctcctg aggcttcttt caaaccaatg atattaggat gttctgctaa
    40561 atttactaat acatggattg gaatcgacat gcctgttcta gaaggaacat tatacaaaat
    40621 aagcggaatc ggtgaggctt ctgcaattgc tgtaaaatga gcagctagtc ctgcatcact
    40681 tgttttgttg tagtaagggg tgatgaccaa aagtgcgtct gcccccaaag aagcaaaatg
    40741 cttcgctttt tcaatcgttt ctttcgtatt attcgttcca gctcctgcaa tcaccggcac
    40801 acggccagcc actttcttta ctgtcagttg aaggatctgc tcctcctcag attgtgtgag
    40861 cgtagaagat tctcctgttg tacctaaaac taataatcca tctgtttgtt catctaaatg
    40921 gaattggatc aatttttcca aaccagcata atctactgat ccctcttcat tcatcggcgt
    40981 gatcagcgcc acgatcgaac cttttatcat cttcattcct ccaatattct tgtccattca
    41041 tgaactgctg ggccagccat ttgtatcgca tcctcaccag agatcatcaa gtctccttgt
    41101 tccaagtgaa caactacctg atctgctgaa attttcccag tttccttagc aaccacataa
    41161 gaggcacagc aacctgtacc gcaagcaagt gtccaaccta ctcctctttc ataggtacgg
    41221 acgatcaatt cttgttcatt gactacttct acaaagttga cattggtttt ttctttgaac
    41281 aaaggatgat gacagatcag ttcacctctt tttctccaga cttcctcacg cgcattttca
    41341 acaaaaacaa cggtatgaat cgttcctaaa aataaactag tgatcgttac tttttcacca
    41401 ttgatctcta tttcctggtt ttttattgga taagtttgat tagctttgat tttttccgga
    41461 gaatcatctg gcactcccat atctacgctg caataaaaag gtgagggatc caacaccgta
    41521 atttttcttg tgcctgctaa tgtctctacg ttgaattcct gttcattcat catgttctga
    41581 tcatttacgt atcgagcaaa acagcgaatg ccatttccgc acatcggcgc gcggctgcca
    41641 tctttgttgt aatagatcat ttccaacgga tcgattttta cggcgatcaa tccgtctata
    41701 tcatattttt cttttgtgca taaagaagcg gctaattgac tataatctgt gtcttctgcg
    41761 taatctaata agataaaatc atttccgcag ccattatatt tatgaaggat cataggaaac
    41821 ttcccccctt agtagatcag aaaatgcttg tctgcgaaca atcagtcttg attggccatt
    41881 attgacaaaa acaactgctg gtcgtgtcgc tcgattgtag ttgctgctca tcgagtatcc
    41941 ataagctccc gtggaataaa cagctaaaag atcacccgat tctgctttcg gcaaataagc
    42001 ttctttaatt accacatcgc ctgattcaca catttttcct gcaatcgtca ctttctctgt
    42061 tttttcttcc gacagttttg ctgctaaatc acaatcgtat tctgcctgat acagagctgg
    42121 acggatatta tctgtcatcc cgccatctac aaagtaatat tttttatgtg gtgtcttttt
    42181 gatgaaacca atcgtgtata gtgttgtacc agcttctcca acaatacttc tacccggttc
    42241 gatcagtaat tgacgaatgg tcagttttcg tttttccaaa gcttcttccg tatattcaat
    42301 cagttttttt agtgcctctt cgactgggat cggctgatca ttttgtgtat aacgaatccc
    42361 aaaccctccg ccaagattca aggaaagagg ttggtcaaaa tcggttaagt aattcgtcaa
    42421 tttgtcgatt tcagctaacc aagcagtaac atcaaaaatt tgtgacccga tatgagcatg
    42481 aaatccttct aactctagat agctgctttc ttttatcaac tgaagggctt gttggcactc
    42541 tttactttca tagctcatgc cgaattttga atcaatatgc gcagtgacga tatattcatg
    42601 agtatgtgct tctactccga cattcaaccg aagcatgata caaatttttt ttaaatagcg
    42661 ttggcttaat tctgctaata attcaacttc catcaaatta tcggcaacaa tatgcaacaa
    42721 atcattttca agtgcataga tcagttcctc tggcgtcttg ttgttaccat ggaagtagat
    42781 agtatctcta ggaaaatcag cttgcaaagc ggtatagatt tccccaccac tgacaacatc
    42841 tagcccgaat ccatattcac gaactaaatg cagcatctca atcgtttgga atgctttaga
    42901 ggcgtaaagc acttttgtat caaaacgttt cgatacaaag cctcgcttga atgacagcat
    42961 cgtttgtctc atcttttctt catcatacac atacagaggt gtcttatact tttctgccaa
    43021 tgtgcttgct gtgactccgc caattatcaa ttctgtcatt ttttcattcc tcttttcact
    43081 ttttttccgt ttttattgga atttcatttg ataaagattt gaattttatt ggaataatat
    43141 taaacaaaat tttctgaatt gtctagtata tttcgaaata tttttttaaa aataagaata
    43201 aaaatggtta taaaaacaaa atacccgaac tattagaagc tatttataca taaaaacata
    43261 aattcaacac tatttagtga tgaaaaaata ttttcgcttc aatatagttc ggaatagttc
    43321 ggatattttc aattttatga atcatttcaa tttcttcata aataagtgca gttttttctt
    43381 tctctagctt ttgccattca tctttcaaaa tataaataaa atcaaatact tttttatttt
    43441 tcaatttgct gattccacaa aaaacctctt gaaaggcaga tccgatgatc tgcctttcaa
    43501 gaggtcaagc tctgttttca aattcttatc aatccgttag cgaattattt tgacgcacgt
    43561 tttacataag aaccttctgc tgtattgatt tccaacaatt catctgtttc gatgaagtca
    43621 ggaacattga ctactaagcc agtttccatt gtcgctggct tacctgaacc agtgactgtt
    43681 gcacctttga tggaaggttg tgtttccaca acacgtaata cgacagttgt tggtaattga
    43741 acgccgatga cttcgctacc gaaaaattgg atttttactt ccatgttttc taggatatat
    43801 ttcatttctt cttctactgt ttctacagga atttcatatt gctcatatgt ttccaaatcc
    43861 atgaagaaag ctgtatcatc catcgtgtac aaatattgta caggttttgt gtcgatatgt
    43921 gctttttcaa atttttcttc tggtctgaac gttgtgtcgt atgttgcacc tgaacggaca
    43981 tctttcagtt tcatacgcat aactgtattt cctttaccag gtttatggtg gcttgcatcc
    44041 agaactttaa tcagtttgcc gtcttgtacg aaagtcatac cggctcttaa atcacttgct
    44101 gagatcatta tttttgctcc ttcttttctt tatcataatc aactttattg tatcagacaa
    44161 ccggccagaa aactagagta ttctctatct tttaaggtct catgcctatt tgtcagaaaa
    44221 ataaacacgg gtaaacgatg ctatcgaaat atttttttct ttcaagcctt tgacttttat
    44281 tttttttcat gctatcgtta ataaaaattt tacttggagg gatacacatg acagtcagca
    44341 accaaaaaca attgaatcgt tgctgttgta tggtgcataa aagcagcata ctatttgtca
    44401 tgtctattaa ttgcccttca tagaatgagt accaggtaac ttatcttttt atctactcaa
    44461 tttttctcta tgtctttcaa ggagctgatt taatagtcag ctcctttttt tattatcttt
    44521 ttttaggagg cccacatgat tatctcgaca attacagaag ctattggttc cacaccgctt
    44581 tatcgctttg acaatcagca ctattctatg cctaaaggtt ctgctattta tgcaaaacta
    44641 gaatatttaa atcctggagg aagtatcaaa gatagattgg gcagttattt gatcaaagaa
    44701 ggatttgccc aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac tgccggaaat
    44761 acaggaattg ggatcgctct ggccgcactc acatacaaat taagaacggt ttttgttgtt
    44821 ccagagaagt ttagcttaga aaaacaacaa ttgatgaaag cattgggagc aaagatcatc
    44881 cattctccta gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa acttgcagag
    44941 gagatatcaa acagctatct gcctcttcaa tttgaaaata aagataatcc tgctgcttac
    45001 tatcatacgt tagggccaga aatctttcat gaaatcaaag aaaatatcca tagttttgta
    45061 gcaggaattg ggagtggtgg aacctttgct ggaacttcta cctttttgaa agaaaaatat
    45121 cccgacattc gaataatcgg tgtagaacca gagggctctg tgttaaatgg tggtgatcca
    45181 gcccctcacg aaattgaggg tatcggtgta gaattcattc cgccgtttct ttctcccctt
    45241 tcaatcaatc agatcgagac aatctctgat gtagaagggt ttaactatac taggcagtta
    45301 gcacgagaac aaggactatt agttggaagt tcgagtggtg cggcttttgc agcagcatta
    45361 agagaaattc gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc agacgcagca
    45421 gaccgctatt tatcaaaaaa catttactta taggaggaaa gaaacatgca cattcaaaca
    45481 aaattgatcc acggaggcat cagtgaagat cccacaacag gagctgtcag tgtacctatt
    45541 tatcaaacat ccacttatcg gcaagatggc gtcggccagc ctaaacaata tgaatattca
    45601 agatctggta atcctactcg ttttgcattg gaagaactga ttgctgattt ggaaggcggt
    45661 gttcgaggat tcgctttttc ttctggtctt tctgggattc atgccgtttt ttctcttttt
    45721 caagctggcg atcatatctt attaggagat gatgtctatg gaggaacatt ccgtctattt
    45781 gacaaagttc ttactaaaaa tggtctcgag tacacgatca tagatacgag taatcttgat
    45841 aaaatagaac aatccatcaa acccaataca aaagcacttt atcttgaaac acctagcaat
    45901 ccgcttctaa agattactga tttagaaaaa agtgccactc tggctcatca acatggattg
    45961 atagttattg ctgacaatac atttgctact ccttattttc aaagaccttt agatctcggt
    46021 tctgatatcg ttgtccatag cggtaccaaa tatctaggcg gacacagcga tgtagtagct
    46081 ggtttagtga cttctaacca taaagatcta gccgatcaga tcggatttta ccaaaatgct
    46141 atcggagccg tacttggtcc ccaagatagc tggttacttc agcgcggaat caagacacta
    46201 agtgttcgta tggaggaaca tcaaaaaaat gcttttgtgg tagctgattt tctattttct
    46261 catccagcag tagaaaaggt ctactatcca ggattacccg atcatgaact ccatggagtt
    46321 gctaagcagc agatgagcgg ctttagcgga atgatttctt ttactttaaa aaatgaagaa
    46381 tctgctattc catttgttga atcactccaa ctatttactt tagcagaaag tttaggtggc
    46441 gtagaaagtt tagtggaaat tccttctgtc atgactcatg cgtctattcc aaaagaaaaa
    46501 cgagaagagg ctggcataaa ggacggcttg atccgtcttt ctgtaggaat tgagtatggt
    46561 caggatctca taaacgattt agctcaggca tttgacagaa tcaagaacta gaagtcccaa
    46621 taaacgactt agcagaaaaa tctaaaaaat atttcagatt gctacacctt ggaaacgata
    46681 agtgttagaa tcaagtagaa agacgttttt gtaaatttca ctaaataatt ctttagtgaa
    46741 aagcatcaaa acgaatgaac aaacagatag gagtcttaca aatgagaatg aatgtcgaaa
    46801 gttttaatct tgaccataca aaagtcaaag caccttatgt acgtgtagct gatcgaaaaa
    46861 cgggtgcgca tggcgatgtc atcattaaat acgacgtccg ctttaaacaa ccaaataaag
    46921 aacacatgga catgccaagt ctacattcac ttgagcactt gactgccgaa ctaatccgca
    46981 atcacgcaga ttacatcgta gactggtctc caatgggatg ccaaacaggc ttttacttga
    47041 ctgtattgaa tcatgaaagc tacgaagaca ttttagatgt tcttgaagca acaatgaaag
    47101 atgttcttga agctactgaa gtacctgcaa gcaatgaaac acaatgtggc tgggctgcca
    47161 gccatacact agaaggtgca caagctttgg ctcgtgaatt tttagcaaaa cgcgatgaat
    47221 ggtctgaagt agaagcatag tttctaaaag aacgtaaaga tgagaagcaa tatagctatc
    47281 ttatcttcgt attttattaa atatttattg tctgaaaaga gttctaaacg ggaaaacaaa
    47341 tagctagttt tcagtttagc gctcttttta ttatataaaa tagaaaggag aaaactttat
    47401 gtctatttat aattttgaaa ctactttaga agacggcact gtctattcat tgaaaaaata
    47461 tgcaggcaaa ccgataatca ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt
    47521 taaacaatta gaagaacttt accaagctta tcagacacaa ggtctggtta tcttaggctt
    47581 tccttccaat caatttaaac aagagctaga ttcagcgact gaagcagcag aagcttgtaa
    47641 aaccacttat ggcgttacct tccctatgca tcaattgatt gacgtaaatg gagaaaatac
    47701 agatcctctt tttacttatc ttaaaaatga agctcctggt actgtcacta aagcaataaa
    47761 atggaatttt actaaatttt tgattgatag aaatggcaaa gtaatcgaac gatttgcacc
    47821 acaaacttct ccattgaaaa tgaccgctgc aattgaatcc gttctttaac ttaataaaaa
    47881 taaaaaaaga tgaactattt ctagttcatc tttttgcctt tgcgtggcga cgtcctactc
    47941 tcacaagggg caacccctca ctacaatcgg cgctaagaag cttaacttct g
//
LOCUS       DACECB010000013        47631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECB010000013 DACECB010000000
VERSION     DACECB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.013"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWO04_000830"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWO04_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0212459.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IWO04_000831"
     CDS             1995..4661
                     /locus_tag="IWO04_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0212460.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNFPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IWO04_000832"
     CDS             5025..5777
                     /locus_tag="IWO04_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0212461.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IWO04_000833"
     CDS             complement(5799..6953)
                     /locus_tag="IWO04_000833"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0212462.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IWO04_000834"
     CDS             7138..9579
                     /locus_tag="IWO04_000834"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0212463.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IWO04_000835"
     CDS             complement(9909..10613)
                     /locus_tag="IWO04_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212464.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IWO04_000836"
     CDS             complement(10800..11045)
                     /locus_tag="IWO04_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212465.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IWO04_000837"
     CDS             complement(11062..11913)
                     /locus_tag="IWO04_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0212466.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IWO04_000838"
     CDS             12079..13083
                     /locus_tag="IWO04_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0212467.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IWO04_000839"
     CDS             complement(13324..13680)
                     /locus_tag="IWO04_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0212468.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IWO04_000840"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IWO04_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0212469.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWO04_000841"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWO04_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0212470.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IWO04_000842"
     CDS             complement(18040..18759)
                     /locus_tag="IWO04_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0212471.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IWO04_000843"
     CDS             18968..19600
                     /locus_tag="IWO04_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0212472.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IWO04_000844"
     CDS             19659..20519
                     /locus_tag="IWO04_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0212473.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IWO04_000845"
     CDS             20626..23019
                     /locus_tag="IWO04_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0212474.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IWO04_000846"
     CDS             23201..25042
                     /locus_tag="IWO04_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0212475.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IWO04_000847"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IWO04_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0212476.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IWO04_000848"
     CDS             26395..28494
                     /locus_tag="IWO04_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0212477.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IWO04_000849"
     CDS             28690..29553
                     /locus_tag="IWO04_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0212478.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..30131
                     /locus_tag="IWO04_000850"
     CDS             29658..30131
                     /locus_tag="IWO04_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0212479.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30227..31114
                     /gene="xerD"
                     /locus_tag="IWO04_000851"
     CDS             30227..31114
                     /gene="xerD"
                     /locus_tag="IWO04_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0212480.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31133..31504
                     /locus_tag="IWO04_000852"
     CDS             31133..31504
                     /locus_tag="IWO04_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0212481.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31474..32271
                     /locus_tag="IWO04_000853"
     CDS             31474..32271
                     /locus_tag="IWO04_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0212482.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32255..32824
                     /gene="scpB"
                     /locus_tag="IWO04_000854"
     CDS             32255..32824
                     /gene="scpB"
                     /locus_tag="IWO04_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0212483.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            32830..33546
                     /locus_tag="IWO04_000855"
     CDS             32830..33546
                     /locus_tag="IWO04_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0212484.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33628..33753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33876..34487
                     /locus_tag="IWO04_000856"
     CDS             33876..34487
                     /locus_tag="IWO04_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0212485.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34865..36388
                     /locus_tag="IWO04_000857"
     CDS             34865..36388
                     /locus_tag="IWO04_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0212486.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36625..37965
                     /locus_tag="IWO04_000858"
     CDS             36625..37965
                     /locus_tag="IWO04_000858"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0212487.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            38118..38855
                     /locus_tag="IWO04_000859"
     CDS             38118..38855
                     /locus_tag="IWO04_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0212488.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(39020..39241)
                     /locus_tag="IWO04_000860"
     CDS             complement(39020..39241)
                     /locus_tag="IWO04_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0212489.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39272..40303
                     /locus_tag="IWO04_000861"
     CDS             39272..40303
                     /locus_tag="IWO04_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212490.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40290..41693
                     /locus_tag="IWO04_000862"
     CDS             40290..41693
                     /locus_tag="IWO04_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0212491.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41759..42394
                     /locus_tag="IWO04_000863"
     CDS             41759..42394
                     /locus_tag="IWO04_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0212492.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42443..43123
                     /locus_tag="IWO04_000864"
     CDS             42443..43123
                     /locus_tag="IWO04_000864"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0212493.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43258..44487
                     /gene="rpsA"
                     /locus_tag="IWO04_000865"
     CDS             43258..44487
                     /gene="rpsA"
                     /locus_tag="IWO04_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0212494.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44669..45979
                     /locus_tag="IWO04_000866"
     CDS             44669..45979
                     /locus_tag="IWO04_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0212495.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46222..46497
                     /locus_tag="IWO04_000867"
     CDS             46222..46497
                     /locus_tag="IWO04_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0212496.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46732..>47631
                     /locus_tag="IWO04_000868"
     CDS             46732..>47631
                     /locus_tag="IWO04_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212497.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEK"
BASE COUNT        15823 a         8288 c        10037 g        13483 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact tcccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtgtttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc
    30121 agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa agactatcag
    30181 aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga aagagcaaat
    30241 caacgattat ctccactact tgaatattga acggggattg tccgccaata cacgacaaag
    30301 ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa ttgcttcatg
    30361 gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg aagaaaaaaa
    30421 agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc agtttttacg
    30481 gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga agaaagcaca
    30541 gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag cacctgatac
    30601 gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg ctactgggat
    30661 gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc ttggactggt
    30721 acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt atgcgatcca
    30781 atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg catctgaaat
    30841 gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct ggaaaaatct
    30901 gaagcagtta gtacgagagg ctgggattta caaagaagta acaccacata cgctgcggca
    30961 tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac aggaattgtt
    31021 aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga aaagaatgac
    31081 ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga agatgttaac
    31141 acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc ataagaaact
    31201 gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag acgattatga
    31261 actttttttc tttacgccag aaggttcaac caacattcaa gggattattg gtatttactg
    31321 ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt atcgcggtga
    31381 gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg tctccatccg
    31441 tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa agaagaaaga
    31501 gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgagggtcc tctcgatctg
    31561 ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat cgctacgatc
    31621 acggagcaat atatgaatta tatccataca atgaaaacac tcgagctagc tgttgcagga
    31681 gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc
    31741 caagaattcg tcacagacga tgaagaatta tgggaagaag atcctcgaga agaattggtc
    31801 actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac agaaaaagca
    31861 gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt agcggatgct
    31921 gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa
    31981 atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca
    32041 acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc gcatggctgt
    32101 actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac ttttatggct
    32161 ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa ctatgaatca
    32221 attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg aacttgaagc
    32281 ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg gactgttaaa
    32341 tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt acactcaaaa
    32401 ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa cgactaaaaa
    32461 agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat tatctcaagc
    32521 cgccgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag ctgaaatcga
    32581 gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc ataaactgat
    32641 tgaagaaaaa ggtagagtag aaggaccagg tcggccgata ttatatggaa caactggcta
    32701 cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac aagaaatgga
    32761 aatttcactt gaagaagacg tgtcaccaga tctattcttt gatggaatga ataaaggaga
    32821 ataattgtaa tggagagatt gcagaaagta attgcccacg ctggcatcac ttctcgtcga
    32881 aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa
    32941 ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc aatttatcaa
    33001 gaagaatacg gttactattt attgtacaaa cctaaaggag ttatttctgc tgttgcagat
    33061 gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg tatctatcct
    33121 gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga tggagatttt
    33181 gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa
    33241 gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat cgaaggacga
    33301 aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa ccacagtatc
    33361 gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct tcaagcagtt
    33421 ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt acaaggccta
    33481 cgtcctgggg attaccgtga actgaacaaa aaagaagtca gcagtttgct gaaccactca
    33541 aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag atatgtataa
    33601 tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga aattcccgac
    33661 cggtggttat agtccacgac ctattttgca aaaaaatagc tgaatcggtg aaattccgat
    33721 accgacagta tagtctggat aaagaagata gagcttattt gacgacgctt gatcaaataa
    33781 ctctaactct attttccctg gaaaatagag ttttttcatt tggagagaaa cgtcaaaaag
    33841 ttcgctgaag aggagtccta acaggaggat ttcaaatgaa gaacacccgt gtacagaaaa
    33901 tggttgcagt agcgatgttt gctgcgatcg cattagtttt acagtatttt gcttttccga
    33961 tcatgccggc ttttagtttt ttgaagatcg attttagtga tatctcggta ttgatcagca
    34021 tgttcttatt cggtccagcg gccggagttg caacagcgtt cttgcgttca gtattacatc
    34081 tgctgactac aggtttttct ccggataatt tggtaggaga cactgctagt ttcttatcaa
    34141 caacgatctt tacattcccg atttattatt tctttagtgt aaagaaacag cataaaggaa
    34201 gaaacaaatt tttaggtgtc attacaggaa ccatcgctct aactattttt atgagtatcg
    34261 caaattattt cgttattacg cctctttact taatgttttt agggctgaac gcaacacaga
    34321 tgctgggcat gtctttagca aactatgtac tgattgggat cgttcctttc aacttgatca
    34381 aaggattcat cgtgagtgct gtctttttag tgctgcatgc gcaacttctt ccatggttaa
    34441 gtcgaaaaca acatcagttc gaacaacgtc atacagcagt caaataaata tcaaaaagat
    34501 caagaaattt cttggtcttt ttttttggaa aaaaatacaa gacatgcaat aatgtaaacg
    34561 ttttatttgt ttgttttgag tatttttttg atttatgcat aaaaatcaat caattctttg
    34621 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
    34681 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
    34741 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
    34801 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
    34861 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
    34921 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
    34981 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
    35041 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
    35101 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
    35161 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
    35221 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
    35281 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
    35341 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
    35401 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
    35461 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
    35521 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
    35581 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
    35641 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
    35701 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
    35761 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
    35821 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
    35881 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
    35941 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
    36001 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
    36061 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
    36121 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
    36181 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
    36241 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
    36301 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
    36361 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
    36421 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
    36481 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
    36541 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
    36601 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
    36661 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
    36721 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
    36781 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
    36841 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
    36901 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
    36961 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
    37021 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
    37081 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
    37141 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
    37201 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
    37261 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
    37321 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
    37381 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
    37441 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
    37501 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
    37561 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
    37621 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
    37681 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
    37741 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
    37801 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
    37861 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
    37921 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
    37981 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
    38041 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
    38101 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
    38161 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
    38221 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
    38281 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
    38341 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
    38401 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
    38461 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
    38521 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
    38581 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
    38641 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
    38701 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
    38761 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
    38821 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
    38881 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
    38941 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
    39001 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
    39061 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
    39121 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
    39181 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
    39241 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
    39301 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
    39361 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
    39421 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
    39481 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
    39541 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
    39601 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
    39661 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
    39721 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
    39781 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
    39841 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
    39901 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
    39961 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
    40021 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
    40081 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
    40141 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
    40201 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
    40261 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
    40321 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
    40381 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
    40441 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
    40501 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
    40561 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
    40621 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
    40681 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
    40741 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
    40801 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
    40861 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
    40921 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
    40981 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
    41041 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
    41101 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
    41161 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
    41221 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
    41281 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
    41341 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
    41401 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
    41461 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
    41521 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
    41581 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
    41641 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
    41701 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
    41761 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
    41821 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
    41881 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
    41941 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
    42001 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
    42061 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
    42121 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
    42181 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
    42241 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
    42301 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
    42361 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
    42421 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
    42481 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
    42541 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
    42601 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
    42661 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
    42721 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
    42781 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
    42841 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
    42901 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
    42961 gaaagagata caggaaagag accattatga ttcaacaagg gaagtctctc cgctgaagca
    43021 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
    43081 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
    43141 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
    43201 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
    43261 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
    43321 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
    43381 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
    43441 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
    43501 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
    43561 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
    43621 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
    43681 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
    43741 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
    43801 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
    43861 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
    43921 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
    43981 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
    44041 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
    44101 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
    44161 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
    44221 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
    44281 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
    44341 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
    44401 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
    44461 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
    44521 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaataaaaat
    44581 acttcgttta attgacaatc tatgcttgaa agttggcaat gcattcgtta aactaagtga
    44641 gattggaaac ttgtgaggag gaaataatat ggcaaatcca acaattgcaa tcgttggccg
    44701 cccgaacgtc ggtaagtcta cgatttttaa ccgcatcgct ggtgagcgta tctcgattgt
    44761 cgaagatact cctggagtaa ctcgtgatcg tatttatgca aaaggtgaat ggttaggtcg
    44821 agaattcagc gtgatcgaca caggcggaat tgatcttggc gatgaacctt ttatggatca
    44881 gatcaagcat caagcagaaa tcgcgattga agaagcagat gtaatcattt gtgtagtcag
    44941 cggacgcgaa ggcgtgacag acgcagatga gatggttgcg aaaattttat acagaagtaa
    45001 taaaccagtg attttagcag tcaataaagt agataatcca gaaatgagaa acgatatcta
    45061 tgaattttat tccttagggc taggcgatcc ttatcctgtt tcaggaagcc atggactagg
    45121 gattggtgat attttagatg aagcagtgaa gtatttctct gaagagactg aagaagagga
    45181 cgacgacacg attaagttta gtttgattgg tcgcccaaat gttggtaaat cctctttgat
    45241 caatgcgatt ttaggagaag aacgtgtaat tgtttcggat attgaaggaa cgacccgaga
    45301 cgcaatcgac acccacttcg tttctgaaag tggtcagaaa ttcttaatga tagatacagc
    45361 tggtatgcgt aaacgcggaa aagtatacga aaatactgaa aaatacagcg tgatgcgtgc
    45421 aatgcgtgcc attgaccgtt ctgatatcgt tttgatggta ctgaatgctg aagaagggat
    45481 tcgggaacaa gacaagcgag tagcgggtta cgcccatgaa gctggtcgtg gaattattat
    45541 cgtcgtgaat aaatgggatc ttgtaaagaa agaaaccaat acaatgcgtg atttcgaaca
    45601 agagattcgt gatgaattcc ggtatcttga ttacgctcca atcgtttttg tttctgcagt
    45661 gactaaacaa cggttggaac gtctgcctga aatgatcgaa caagtaagta tgaaccagaa
    45721 tcttcgtatc tcttcggctg tgttgaatga tatcattatg gatgctgtag caatcaatcc
    45781 aacaccaaca gataaaggaa aacgcctgaa gattttttat gctacacaag tcgctgtcaa
    45841 accacctaca tttgtcgttt ttgtcaatga agaggaattg atgcattttt cttatgaacg
    45901 tttcttagaa aatcaaatca gaaaagcatt tacttttgaa gggacgtcca tccgaattat
    45961 ccctcgtagg agaaaatagg cattttacca tatcgacgtt tttttttcaa aagaaaaaga
    46021 cagtttcgtt tgttacgttt ctatgactaa aaaaagcatg tgaaaattgg taaaaacctt
    46081 gctattaagc ggttcatatg atatcgtgat aacagaatat tgaagttaat caatatttct
    46141 ttttaaactt ctggaccact cagaaagttt taactaaatg atatttctat catatctctt
    46201 gtggaggagg tgaatctatc catggcaaac aaagcagaat taatcgaaaa agttgcttca
    46261 gctactgatt taactaaaaa agatgcaact gcagcagtag atgctgtatt ttcaactatt
    46321 caagatgctt tagctaacgg cgaaaaagtt caattaatcg gttttggtaa ctttgaagtt
    46381 cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg gtgaagaaat cgaaattcca
    46441 gctagcaaag tacctgcatt caaaccaggt aaagcattaa aagacgctgt taaataagac
    46501 agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt ttctttttat ttcctagcag
    46561 aaaaggaata aataagagac agagattccg aatccgtatg tcatacatag aaaacacatg
    46621 tattttattt aatttttatt tatttgcaag ttatgaggtg agctttgctc ttatcttttc
    46681 tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg tatggaactt
    46741 gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct agtcagtcag
    46801 ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg ctattatgat
    46861 ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga ctggtatatt
    46921 tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga tttcgtcaaa
    46981 cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg gatcatggat
    47041 ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat gatccctgta
    47101 tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca gctaccaaat
    47161 cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca aacaatcagc
    47221 gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg cggaatgatt
    47281 tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa gcaattaacg
    47341 actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt agaggcagaa
    47401 aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga aaatggccat
    47461 ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt gtggaatctc
    47521 caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga cttgcttggc
    47581 tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa a
//
LOCUS       DACECB010000014        45761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.014, whole genome shotgun sequence.
ACCESSION   DACECB010000014 DACECB010000000
VERSION     DACECB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.014"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..730
                     /locus_tag="IWO04_000869"
     CDS             8..730
                     /locus_tag="IWO04_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0212498.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="IWO04_000870"
     CDS             788..2827
                     /locus_tag="IWO04_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0212499.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="IWO04_000871"
     CDS             complement(2936..4087)
                     /locus_tag="IWO04_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0212500.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="IWO04_000872"
     CDS             complement(4241..4771)
                     /locus_tag="IWO04_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212501.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="IWO04_000873"
     CDS             complement(4870..5220)
                     /locus_tag="IWO04_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0212502.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="IWO04_000874"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="IWO04_000874"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0212503.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="IWO04_000875"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="IWO04_000875"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0212504.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="IWO04_000876"
     CDS             complement(7623..8285)
                     /locus_tag="IWO04_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0212505.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="IWO04_000877"
     CDS             8469..10115
                     /locus_tag="IWO04_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0212506.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="IWO04_000878"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="IWO04_000878"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0212507.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="IWO04_000879"
     CDS             complement(11296..12849)
                     /locus_tag="IWO04_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0212508.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12997..14166
                     /locus_tag="IWO04_000880"
     CDS             12997..14166
                     /locus_tag="IWO04_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0212509.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14292..15371)
                     /locus_tag="IWO04_000881"
     CDS             complement(14292..15371)
                     /locus_tag="IWO04_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0212510.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15480..15920)
                     /locus_tag="IWO04_000882"
     CDS             complement(15480..15920)
                     /locus_tag="IWO04_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0212511.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15977..16387)
                     /locus_tag="IWO04_000883"
     CDS             complement(15977..16387)
                     /locus_tag="IWO04_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0212512.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16568..17002)
                     /locus_tag="IWO04_000884"
     CDS             complement(16568..17002)
                     /locus_tag="IWO04_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0212513.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17102..17950)
                     /locus_tag="IWO04_000885"
     CDS             complement(17102..17950)
                     /locus_tag="IWO04_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0212514.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18339..20105
                     /locus_tag="IWO04_000886"
     CDS             18339..20105
                     /locus_tag="IWO04_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0212515.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20207..21466
                     /locus_tag="IWO04_000887"
     CDS             20207..21466
                     /locus_tag="IWO04_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0212516.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21613..22914
                     /locus_tag="IWO04_000888"
     CDS             21613..22914
                     /locus_tag="IWO04_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0212517.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22917..23768
                     /locus_tag="IWO04_000889"
     CDS             22917..23768
                     /locus_tag="IWO04_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0212518.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23781..24596
                     /locus_tag="IWO04_000890"
     CDS             23781..24596
                     /locus_tag="IWO04_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0212519.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24747..25913
                     /locus_tag="IWO04_000891"
     CDS             24747..25913
                     /locus_tag="IWO04_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0212520.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26143..26790
                     /locus_tag="IWO04_000892"
     CDS             26143..26790
                     /locus_tag="IWO04_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0212521.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26874..27818
                     /locus_tag="IWO04_000893"
     CDS             26874..27818
                     /locus_tag="IWO04_000893"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0212522.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            27953..29404
                     /locus_tag="IWO04_000894"
     CDS             27953..29404
                     /locus_tag="IWO04_000894"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0212523.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29425..30261
                     /locus_tag="IWO04_000895"
     CDS             29425..30261
                     /locus_tag="IWO04_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0212524.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30261..30920
                     /locus_tag="IWO04_000896"
     CDS             30261..30920
                     /locus_tag="IWO04_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0212525.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31046..31735
                     /locus_tag="IWO04_000897"
     CDS             31046..31735
                     /locus_tag="IWO04_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212526.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31741..32697
                     /locus_tag="IWO04_000898"
     CDS             31741..32697
                     /locus_tag="IWO04_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0212527.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32741..33244
                     /locus_tag="IWO04_000899"
     CDS             32741..33244
                     /locus_tag="IWO04_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212528.1"
                     /translation="MYIRQTRKEEVVEIINAAADFIASQGSPQWQNGQKPTKETILND
                     IERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAESTRGK
                     KLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHGERK
                     AYQIIIN"
     gene            complement(33500..34048)
                     /locus_tag="IWO04_000900"
     CDS             complement(33500..34048)
                     /locus_tag="IWO04_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0212529.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34137..35318)
                     /locus_tag="IWO04_000901"
     CDS             complement(34137..35318)
                     /locus_tag="IWO04_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0212530.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35554..37062)
                     /locus_tag="IWO04_000902"
     CDS             complement(35554..37062)
                     /locus_tag="IWO04_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0212531.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37221..37412
                     /locus_tag="IWO04_000903"
     CDS             37221..37412
                     /locus_tag="IWO04_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212532.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37615..38532
                     /locus_tag="IWO04_000904"
     CDS             37615..38532
                     /locus_tag="IWO04_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0212533.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38525..39598
                     /locus_tag="IWO04_000905"
     CDS             38525..39598
                     /locus_tag="IWO04_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212534.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39746..40264
                     /locus_tag="IWO04_000906"
     CDS             39746..40264
                     /locus_tag="IWO04_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212535.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40245..40637
                     /locus_tag="IWO04_000907"
     CDS             40245..40637
                     /locus_tag="IWO04_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0212536.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40713..41648
                     /locus_tag="IWO04_000908"
     CDS             40713..41648
                     /locus_tag="IWO04_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0212537.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41781..42560
                     /locus_tag="IWO04_000909"
     CDS             41781..42560
                     /locus_tag="IWO04_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0212538.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42553..42870
                     /locus_tag="IWO04_000910"
     CDS             42553..42870
                     /locus_tag="IWO04_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0212539.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43114..44724
                     /locus_tag="IWO04_000911"
     CDS             43114..44724
                     /locus_tag="IWO04_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0212540.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            44881..45729
                     /locus_tag="IWO04_000912"
     CDS             44881..45729
                     /locus_tag="IWO04_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0212541.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15176 a         8249 c         8800 g        13536 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcctgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    12961 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    13021 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    13081 atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    13141 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    13201 atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca
    13261 tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa
    13321 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    13381 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat
    13441 agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa
    13501 gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca
    13561 gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca
    13621 gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca
    13681 gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13741 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga ggtagatcca
    13801 gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca
    13861 gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat
    13921 ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc
    13981 gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag tcctgaaacg
    14041 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    14101 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    14161 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    14221 acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    14281 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    14341 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    14401 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    14461 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    14521 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    14581 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    14641 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtgttt
    14701 aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    14761 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    14821 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    14881 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    14941 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    15001 tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt
    15061 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    15121 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    15181 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    15241 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    15301 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    15361 acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac
    15421 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
    15481 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
    15541 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
    15601 gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat tctttcatca
    15661 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
    15721 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
    15781 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
    15841 gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca tacttgatta
    15901 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
    15961 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
    16021 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
    16081 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
    16141 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
    16201 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
    16261 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
    16321 cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
    16381 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
    16441 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
    16501 gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac taatctcatc
    16561 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt acttcacgat
    16621 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
    16681 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
    16741 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
    16801 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtgccat
    16861 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
    16921 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
    16981 ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
    17041 ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
    17101 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
    17161 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
    17221 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
    17281 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
    17341 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
    17401 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaacaa
    17461 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
    17521 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
    17581 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
    17641 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa aacaagcttc
    17701 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
    17761 cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct tccagtaata
    17821 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
    17881 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
    17941 tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta ttataactta
    18001 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
    18061 ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt actttagaaa
    18121 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagatt
    18181 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
    18241 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct ctatcactta
    18301 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
    18361 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
    18421 gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca tataccataa
    18481 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
    18541 atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat gcgttccatg
    18601 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
    18661 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
    18721 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
    18781 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
    18841 cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat ttggattact
    18901 tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
    18961 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
    19021 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
    19081 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
    19141 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
    19201 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
    19261 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
    19321 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
    19381 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
    19441 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
    19501 attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
    19561 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
    19621 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
    19681 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
    19741 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
    19801 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
    19861 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtatatg
    19921 atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc tatgtttact
    19981 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa
    20041 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
    20101 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
    20161 ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga aaaaaagaag
    20221 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
    20281 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
    20341 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
    20401 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
    20461 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
    20521 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
    20581 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
    20641 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
    20701 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
    20761 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
    20821 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
    20881 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
    20941 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
    21001 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
    21061 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
    21121 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
    21181 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
    21241 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
    21301 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
    21361 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
    21421 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
    21481 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
    21541 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
    21601 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
    21661 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
    21721 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
    21781 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
    21841 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
    21901 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
    21961 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
    22021 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
    22081 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
    22141 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
    22201 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
    22261 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
    22321 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
    22381 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
    22441 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
    22501 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
    22561 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
    22621 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
    22681 gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc cggaagcgta
    22741 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    22801 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    22861 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    22921 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    22981 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    23041 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    23101 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    23161 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    23221 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    23281 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    23341 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    23401 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    23461 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    23521 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    23581 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    23641 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    23701 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    23761 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    23821 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    23881 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    23941 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    24001 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    24061 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    24121 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    24181 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    24241 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    24301 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    24361 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    24421 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    24481 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    24541 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    24601 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    24661 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    24721 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    24781 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    24841 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    24901 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
    24961 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    25021 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    25081 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    25141 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    25201 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    25261 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    25321 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    25381 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    25441 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    25501 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    25561 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    25621 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    25681 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    25741 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    25801 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    25861 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    25921 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    25981 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
    26041 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
    26101 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
    26161 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
    26221 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
    26281 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
    26341 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
    26401 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
    26461 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
    26521 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
    26581 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
    26641 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
    26701 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
    26761 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
    26821 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
    26881 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
    26941 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
    27001 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
    27061 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
    27121 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
    27181 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
    27241 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
    27301 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
    27361 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
    27421 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
    27481 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
    27541 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
    27601 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
    27661 tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa gggccggaaa
    27721 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
    27781 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
    27841 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
    27901 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
    27961 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
    28021 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
    28081 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    28141 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    28201 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    28261 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    28321 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    28381 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    28441 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    28501 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    28561 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    28621 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    28681 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    28741 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    28801 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    28861 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    28921 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    28981 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    29041 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    29101 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    29161 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    29221 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    29281 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    29341 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    29401 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    29461 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    29521 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    29581 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    29641 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    29701 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    29761 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    29821 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    29881 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    29941 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    30001 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    30061 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    30121 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    30181 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    30241 gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc atgtggactt
    30301 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    30361 ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    30421 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    30481 tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    30541 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    30601 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    30661 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    30721 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    30781 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    30841 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    30901 ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    30961 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    31021 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    31081 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    31141 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    31201 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    31261 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    31321 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    31381 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    31441 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    31501 aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat tgaaaagacg
    31561 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    31621 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    31681 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    31741 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    31801 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    31861 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    31921 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    31981 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    32041 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    32101 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    32161 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    32221 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    32281 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    32341 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    32401 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    32461 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    32521 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    32581 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    32641 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    32701 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    32761 gaaagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    32821 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    32881 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    32941 aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    33001 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    33061 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    33121 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    33181 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    33241 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    33301 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    33361 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    33421 aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta
    33481 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    33541 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    33601 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    33661 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    33721 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    33781 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    33841 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    33901 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    33961 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    34021 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    34081 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat
    34141 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    34201 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    34261 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    34321 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    34381 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    34441 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    34501 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    34561 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    34621 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    34681 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    34741 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    34801 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    34861 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
    34921 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    34981 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    35041 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    35101 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    35161 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    35221 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    35281 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    35341 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    35401 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    35461 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    35521 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    35581 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    35641 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    35701 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    35761 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    35821 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    35881 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    35941 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    36001 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    36061 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    36121 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    36181 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    36241 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    36301 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    36361 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    36421 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    36481 gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc
    36541 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    36601 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    36661 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    36721 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    36781 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    36841 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    36901 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc
    36961 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    37021 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    37081 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    37141 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    37201 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    37261 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    37321 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    37381 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    37441 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    37501 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    37561 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    37621 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag
    37681 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    37741 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    37801 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    37861 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    37921 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    37981 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    38041 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    38101 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    38161 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    38221 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    38281 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    38341 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    38401 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    38461 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    38521 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    38581 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    38641 aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac
    38701 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    38761 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    38821 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    38881 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    38941 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    39001 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    39061 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    39121 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    39181 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    39241 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    39301 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    39361 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    39421 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    39481 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    39541 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    39601 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    39661 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    39721 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    39781 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    39841 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    39901 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    39961 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    40021 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    40081 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    40141 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    40201 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    40261 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    40321 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    40381 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt
    40441 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    40501 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    40561 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    40621 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    40681 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    40741 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    40801 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    40861 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    40921 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    40981 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    41041 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    41101 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    41161 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    41221 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    41281 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    41341 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    41401 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    41461 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    41521 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    41581 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    41641 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    41701 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    41761 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    41821 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    41881 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    41941 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    42001 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    42061 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    42121 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    42181 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    42241 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    42301 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    42361 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    42421 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    42481 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    42541 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    42601 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    42661 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    42721 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    42781 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    42841 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    42901 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    42961 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    43021 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    43081 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    43141 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    43201 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    43261 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    43321 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    43381 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    43441 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    43501 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    43561 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    43621 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    43681 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    43741 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    43801 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    43861 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    43921 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    43981 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    44041 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    44101 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    44161 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    44221 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    44281 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    44341 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    44401 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    44461 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    44521 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    44581 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    44641 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    44701 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    44761 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    44821 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    44881 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    44941 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    45001 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    45061 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    45121 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    45181 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    45241 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    45301 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt
    45361 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    45421 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    45481 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    45541 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    45601 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    45661 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    45721 gcgcaataat tccttttatt caaaaaacat accaaaaata t
//
LOCUS       DACECB010000015        45748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECB010000015 DACECB010000000
VERSION     DACECB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.015"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..544
                     /locus_tag="IWO04_000913"
     CDS             101..544
                     /locus_tag="IWO04_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0212542.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IWO04_000914"
     CDS             complement(550..1734)
                     /locus_tag="IWO04_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0212543.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IWO04_000915"
     CDS             2002..2700
                     /locus_tag="IWO04_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0212544.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IWO04_000916"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IWO04_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0212545.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IWO04_000917"
     CDS             complement(3510..4043)
                     /locus_tag="IWO04_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212546.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IWO04_000918"
     CDS             complement(4340..6721)
                     /locus_tag="IWO04_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0212547.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWO04_000919"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWO04_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0212548.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IWO04_000920"
     CDS             7422..7961
                     /locus_tag="IWO04_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0212549.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IWO04_000921"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IWO04_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0212550.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IWO04_000922"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO04_000922"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IWO04_000923"
     CDS             9068..10234
                     /locus_tag="IWO04_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0212551.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IWO04_000924"
     CDS             10280..11776
                     /locus_tag="IWO04_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0212552.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IWO04_000925"
     CDS             11857..12540
                     /locus_tag="IWO04_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0212553.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IWO04_000926"
     CDS             13185..14375
                     /locus_tag="IWO04_000926"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0212554.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IWO04_000927"
     CDS             14477..15430
                     /locus_tag="IWO04_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0212555.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IWO04_000928"
     CDS             15499..16848
                     /locus_tag="IWO04_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0212556.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IWO04_000929"
     CDS             complement(17031..17354)
                     /locus_tag="IWO04_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212557.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IWO04_000930"
     CDS             17483..18646
                     /locus_tag="IWO04_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212558.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IWO04_000931"
     CDS             18716..20065
                     /locus_tag="IWO04_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0212559.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IWO04_000932"
     CDS             complement(20110..20541)
                     /locus_tag="IWO04_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212560.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IWO04_000933"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IWO04_000933"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0212561.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IWO04_000934"
     CDS             23771..24367
                     /locus_tag="IWO04_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0212562.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IWO04_000935"
     CDS             24489..25637
                     /locus_tag="IWO04_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0212563.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IWO04_000936"
     CDS             complement(25675..26088)
                     /locus_tag="IWO04_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0212564.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IWO04_000937"
     CDS             26167..26598
                     /locus_tag="IWO04_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0212565.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IWO04_000938"
     CDS             complement(26697..26930)
                     /locus_tag="IWO04_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0212566.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IWO04_000939"
     CDS             27113..28783
                     /locus_tag="IWO04_000939"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0212567.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IWO04_000940"
     CDS             28930..29316
                     /locus_tag="IWO04_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212568.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IWO04_000941"
     CDS             29303..30001
                     /locus_tag="IWO04_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0212569.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IWO04_000942"
     CDS             30006..30470
                     /locus_tag="IWO04_000942"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0212570.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IWO04_000943"
     CDS             30467..31378
                     /locus_tag="IWO04_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0212571.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IWO04_000944"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IWO04_000944"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0212572.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IWO04_000945"
     CDS             32166..33449
                     /locus_tag="IWO04_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0212573.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IWO04_000946"
     CDS             33462..34388
                     /locus_tag="IWO04_000946"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0212574.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IWO04_000947"
     CDS             34407..35687
                     /locus_tag="IWO04_000947"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0212575.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IWO04_000948"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IWO04_000948"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0212576.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IWO04_000949"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IWO04_000949"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0212577.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IWO04_000950"
     CDS             40012..40788
                     /locus_tag="IWO04_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0212578.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IWO04_000951"
     CDS             40785..41717
                     /locus_tag="IWO04_000951"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0212579.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IWO04_000952"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IWO04_000952"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0212580.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IWO04_000953"
     CDS             42437..43066
                     /locus_tag="IWO04_000953"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0212581.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IWO04_000954"
     CDS             43054..43368
                     /locus_tag="IWO04_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212582.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IWO04_000955"
     CDS             43406..44047
                     /locus_tag="IWO04_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0212583.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IWO04_000956"
     CDS             44078..44782
                     /locus_tag="IWO04_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0212584.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IWO04_000957"
     CDS             44779..45681
                     /locus_tag="IWO04_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0212585.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        15005 a         7997 c         9883 g        12863 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat cgttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattt
//
LOCUS       DACECB010000016        43449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECB010000016 DACECB010000000
VERSION     DACECB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.016"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(113..637)
                     /locus_tag="IWO04_000958"
     CDS             complement(113..637)
                     /locus_tag="IWO04_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212586.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="IWO04_000959"
     CDS             complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="IWO04_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0212587.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1226..2206)
                     /gene="pta"
                     /locus_tag="IWO04_000960"
     CDS             complement(1226..2206)
                     /gene="pta"
                     /locus_tag="IWO04_000960"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0212588.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2359..3039)
                     /locus_tag="IWO04_000961"
     CDS             complement(2359..3039)
                     /locus_tag="IWO04_000961"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0212589.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3165..4034
                     /locus_tag="IWO04_000962"
     CDS             3165..4034
                     /locus_tag="IWO04_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0212590.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4060..4575
                     /locus_tag="IWO04_000963"
     CDS             4060..4575
                     /locus_tag="IWO04_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212591.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4622..5185)
                     /locus_tag="IWO04_000964"
     CDS             complement(4622..5185)
                     /locus_tag="IWO04_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212592.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5240..5419)
                     /locus_tag="IWO04_000965"
     CDS             complement(5240..5419)
                     /locus_tag="IWO04_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212593.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5496..6005)
                     /locus_tag="IWO04_000966"
     CDS             complement(5496..6005)
                     /locus_tag="IWO04_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212594.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6101..6481)
                     /locus_tag="IWO04_000967"
     CDS             complement(6101..6481)
                     /locus_tag="IWO04_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0212595.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6811..7560
                     /locus_tag="IWO04_000968"
     CDS             6811..7560
                     /locus_tag="IWO04_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0212596.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7744..9021)
                     /locus_tag="IWO04_000969"
     CDS             complement(7744..9021)
                     /locus_tag="IWO04_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212597.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9100..9744)
                     /gene="pcp"
                     /locus_tag="IWO04_000970"
     CDS             complement(9100..9744)
                     /gene="pcp"
                     /locus_tag="IWO04_000970"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0212598.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9943..10887)
                     /locus_tag="IWO04_000971"
     CDS             complement(9943..10887)
                     /locus_tag="IWO04_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0212599.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10889..11575)
                     /locus_tag="IWO04_000972"
     CDS             complement(10889..11575)
                     /locus_tag="IWO04_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0212600.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12049..12369
                     /locus_tag="IWO04_000973"
     CDS             12049..12369
                     /locus_tag="IWO04_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0212601.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12523..13428
                     /locus_tag="IWO04_000974"
     CDS             12523..13428
                     /locus_tag="IWO04_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212602.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13425..14198
                     /locus_tag="IWO04_000975"
     CDS             13425..14198
                     /locus_tag="IWO04_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0212603.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14379..15803)
                     /locus_tag="IWO04_000976"
     CDS             complement(14379..15803)
                     /locus_tag="IWO04_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0212604.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16014..16460
                     /gene="nrdI"
                     /locus_tag="IWO04_000977"
     CDS             16014..16460
                     /gene="nrdI"
                     /locus_tag="IWO04_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0212605.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16525..18507)
                     /locus_tag="IWO04_000978"
     CDS             complement(16525..18507)
                     /locus_tag="IWO04_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0212606.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18521..19417)
                     /gene="murQ"
                     /locus_tag="IWO04_000979"
     CDS             complement(18521..19417)
                     /gene="murQ"
                     /locus_tag="IWO04_000979"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0212607.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19420..20481)
                     /locus_tag="IWO04_000980"
     CDS             complement(19420..20481)
                     /locus_tag="IWO04_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0212608.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20692..21219)
                     /locus_tag="IWO04_000981"
     CDS             complement(20692..21219)
                     /locus_tag="IWO04_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0212609.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21286..22788)
                     /locus_tag="IWO04_000982"
     CDS             complement(21286..22788)
                     /locus_tag="IWO04_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0212610.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23046..24167)
                     /locus_tag="IWO04_000983"
     CDS             complement(23046..24167)
                     /locus_tag="IWO04_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0212611.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24293..25234
                     /locus_tag="IWO04_000984"
     CDS             24293..25234
                     /locus_tag="IWO04_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0212612.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25384..26280)
                     /locus_tag="IWO04_000985"
     CDS             complement(25384..26280)
                     /locus_tag="IWO04_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0212613.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26549..27889
                     /locus_tag="IWO04_000986"
     CDS             26549..27889
                     /locus_tag="IWO04_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0212614.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28107..28550
                     /locus_tag="IWO04_000987"
     CDS             28107..28550
                     /locus_tag="IWO04_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0212615.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29054..30472)
                     /gene="nhaC"
                     /locus_tag="IWO04_000988"
     CDS             complement(29054..30472)
                     /gene="nhaC"
                     /locus_tag="IWO04_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0212616.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30875..32098)
                     /locus_tag="IWO04_000989"
     CDS             complement(30875..32098)
                     /locus_tag="IWO04_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0212617.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32129..32968)
                     /locus_tag="IWO04_000990"
     CDS             complement(32129..32968)
                     /locus_tag="IWO04_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0212618.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32985..33665)
                     /locus_tag="IWO04_000991"
     CDS             complement(32985..33665)
                     /locus_tag="IWO04_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212619.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33667..34740)
                     /locus_tag="IWO04_000992"
     CDS             complement(33667..34740)
                     /locus_tag="IWO04_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212620.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35387..36058)
                     /locus_tag="IWO04_000993"
     CDS             complement(35387..36058)
                     /locus_tag="IWO04_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0212621.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36145..38823)
                     /gene="mgtA"
                     /locus_tag="IWO04_000994"
     CDS             complement(36145..38823)
                     /gene="mgtA"
                     /locus_tag="IWO04_000994"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0212622.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39482..39919
                     /gene="ndk"
                     /locus_tag="IWO04_000995"
     CDS             39482..39919
                     /gene="ndk"
                     /locus_tag="IWO04_000995"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0212623.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39941..40807)
                     /locus_tag="IWO04_000996"
     CDS             complement(39941..40807)
                     /locus_tag="IWO04_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0212624.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40967..43060)
                     /locus_tag="IWO04_000997"
     CDS             complement(40967..43060)
                     /locus_tag="IWO04_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0212625.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12546 a         9102 c         7667 g        14134 t
ORIGIN      
        1 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
       61 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
      121 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      181 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      241 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      301 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      361 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      421 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      481 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      541 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      601 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      661 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      721 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      781 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      841 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      901 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      961 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     1021 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1081 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1141 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1201 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1261 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1321 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1381 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1441 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1501 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1561 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1621 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1681 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1741 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1801 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1861 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1921 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1981 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     2041 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2101 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2161 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2221 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     2281 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     2341 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     2401 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     2461 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     2521 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     2581 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     2641 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     2701 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     2761 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     2821 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     2881 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     2941 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     3001 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     3061 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     3121 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     3181 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     3241 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     3301 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     3361 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     3421 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     3481 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     3541 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     3601 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     3661 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     3721 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     3781 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     3841 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     3901 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     3961 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     4021 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     4081 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     4141 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     4201 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     4261 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     4321 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     4381 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     4441 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     4501 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     4561 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     4621 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     4681 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     4741 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     4801 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     4861 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     4921 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     4981 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     5041 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     5101 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     5161 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     5221 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     5281 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     5341 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     5401 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     5461 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     5521 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     5581 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     5641 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     5701 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     5761 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     5821 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     5881 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     5941 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     6001 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     6061 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     6121 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
     6181 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
     6241 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
     6301 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
     6361 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
     6421 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
     6481 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
     6541 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
     6601 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
     6661 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
     6721 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
     6781 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
     6841 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag
     6901 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
     6961 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
     7021 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
     7081 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
     7141 tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca
     7201 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
     7261 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
     7321 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
     7381 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
     7441 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
     7501 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
     7561 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     7621 aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
     7681 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat
     7741 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
     7801 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
     7861 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
     7921 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
     7981 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
     8041 ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat
     8101 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
     8161 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
     8221 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
     8281 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
     8341 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
     8401 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
     8461 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
     8521 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
     8581 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
     8641 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
     8701 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
     8761 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
     8821 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
     8881 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
     8941 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
     9001 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
     9061 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
     9121 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
     9181 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
     9241 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
     9301 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
     9361 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
     9421 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
     9481 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
     9541 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
     9601 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
     9661 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
     9721 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
     9781 tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata
     9841 acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag
     9901 aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat
     9961 ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc
    10021 gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg
    10081 tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt
    10141 cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc
    10201 aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc
    10261 gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac
    10321 tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg
    10381 ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg
    10441 agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc
    10501 acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat
    10561 aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg
    10621 gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc
    10681 accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca
    10741 gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt
    10801 gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa
    10861 aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg
    10921 ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca
    10981 ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg
    11041 ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc
    11101 agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt
    11161 ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc
    11221 cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa
    11281 tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt
    11341 ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca
    11401 tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac
    11461 cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa
    11521 atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt
    11581 ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt
    11641 tgttttctca cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa
    11701 attatcttac ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa
    11761 ataaggtcta gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa
    11821 ttgcaaagca ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt
    11881 actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat
    11941 acaatctgcc accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg
    12001 attttcctct ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg
    12061 gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat
    12121 ggtttttcta aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac
    12181 tttttgtcaa aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg
    12241 attggcgcag ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt
    12301 tccacttggt tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc
    12361 ggccactaga cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt
    12421 caattttttt atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc
    12481 aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc
    12541 caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta
    12601 tcccctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg
    12661 aaagctattt taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag
    12721 atttctccct ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt
    12781 atctatccat tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta
    12841 tctgctatcg acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg
    12901 aaatcctact cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat
    12961 ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat
    13021 gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac
    13081 ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc
    13141 atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct
    13201 acaacaaatg atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat
    13261 cctgataaaa tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta
    13321 ttgtctgcac aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct
    13381 tctctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa
    13441 aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag
    13501 ttgttttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag
    13561 ttgaaatgac tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag
    13621 ctgcaagcac gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc
    13681 gaaagtattc tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg
    13741 tttatttata ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt
    13801 cgatttcctt cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt
    13861 atactttggg tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt
    13921 gctttatcaa tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt
    13981 caatcatatc tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc
    14041 cgatcagtat gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga
    14101 ctcatctttc tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag
    14161 cattaggata tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg
    14221 tgaaagagac gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa
    14281 aaaggagctg tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg
    14341 acaggctcac aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg
    14401 gcaaataaaa ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct
    14461 aagtggatat gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg
    14521 cctggcaaga aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc
    14581 catacttgaa aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga
    14641 taagaaagtt tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta
    14701 ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca
    14761 ttcgtacata acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt
    14821 ccccatgctt taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag
    14881 atcgtccatg agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact
    14941 gctccccatt taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc
    15001 gtttgcagct gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata
    15061 ataatcaagc cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct
    15121 cgtgaaccta cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg
    15181 caactcaata cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg
    15241 ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat
    15301 aaaaataatg gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg
    15361 actccccgat tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat
    15421 gccagatttt gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat
    15481 gttgcaaaag ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa
    15541 ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga
    15601 cgttttttat tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt
    15661 acccaactga aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat
    15721 acgccacctc caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct
    15781 tttttctgtt gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa
    15841 agtataacaa acctttttta tttttactta attaataaaa actttaatag aaatataatt
    15901 ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac
    15961 caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca
    16021 ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg
    16081 ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg
    16141 aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag
    16201 acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat
    16261 atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa
    16321 acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt
    16381 tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt
    16441 tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct
    16501 ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt
    16561 tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac
    16621 gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag
    16681 cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa
    16741 gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc
    16801 aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag aatagatatg
    16861 tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc
    16921 acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact
    16981 ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt
    17041 cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa
    17101 gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat
    17161 caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat
    17221 tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag
    17281 aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc
    17341 tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc
    17401 tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt
    17461 ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat
    17521 cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca
    17581 gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat
    17641 ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc
    17701 aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac
    17761 acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg
    17821 tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg
    17881 tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat
    17941 cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt
    18001 tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac
    18061 aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt
    18121 agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg
    18181 gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc
    18241 taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc
    18301 gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa
    18361 ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg
    18421 gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc
    18481 ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa
    18541 atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca
    18601 tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt
    18661 aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg
    18721 ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc atactgatag
    18781 tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg
    18841 tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg
    18901 cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc
    18961 caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg
    19021 tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga
    19081 cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta
    19141 caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg
    19201 ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg
    19261 gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca
    19321 ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc
    19381 gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat
    19441 aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac
    19501 tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc
    19561 acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc
    19621 tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac
    19681 atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc
    19741 atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc
    19801 ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa
    19861 tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc
    19921 ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc
    19981 ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg
    20041 acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa
    20101 ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga
    20161 ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat
    20221 tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga
    20281 aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg
    20341 cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa
    20401 gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat
    20461 aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga
    20521 ctagagtata tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca
    20581 tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct
    20641 aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata attactcata
    20701 agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg
    20761 aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc
    20821 ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga
    20881 ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt
    20941 gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag
    21001 cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga
    21061 atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc
    21121 ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc
    21181 gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc
    21241 aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag
    21301 agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat
    21361 ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata
    21421 attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc
    21481 cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc
    21541 catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc
    21601 aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag
    21661 atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg
    21721 gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc
    21781 gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac
    21841 gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc
    21901 gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc
    21961 tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct
    22021 agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga
    22081 ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc
    22141 attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt
    22201 cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc
    22261 gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc
    22321 cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa
    22381 atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag
    22441 tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc
    22501 gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt
    22561 atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata
    22621 aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat
    22681 aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc
    22741 ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct
    22801 cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac
    22861 acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt
    22921 ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat
    22981 gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt
    23041 gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt
    23101 ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata
    23161 cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa
    23221 atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt
    23281 ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg
    23341 atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct
    23401 cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct
    23461 aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag
    23521 aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt
    23581 tgacctgtat atggattggt aacatctgca gcgataatca attttacttt agaaacaaga
    23641 acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac
    23701 ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt
    23761 cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct
    23821 cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct
    23881 aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat
    23941 aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt
    24001 cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca
    24061 ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat
    24121 gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac
    24181 tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt
    24241 ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat
    24301 cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg
    24361 aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg
    24421 gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga
    24481 agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca
    24541 attagatgat atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg
    24601 tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat
    24661 cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt
    24721 aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa
    24781 agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta
    24841 ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga
    24901 ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt
    24961 atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta
    25021 ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta
    25081 ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg
    25141 gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga cccaattagt
    25201 ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga
    25261 atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct
    25321 catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt
    25381 tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc
    25441 gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag
    25501 gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag
    25561 caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac
    25621 tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc
    25681 ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt
    25741 aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc
    25801 tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc
    25861 accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa
    25921 tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc
    25981 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg
    26041 attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc
    26101 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc
    26161 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    26221 ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat
    26281 cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    26341 gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga
    26401 aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg
    26461 aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata taaattttga
    26521 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
    26581 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
    26641 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
    26701 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
    26761 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
    26821 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
    26881 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
    26941 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
    27001 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
    27061 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
    27121 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
    27181 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
    27241 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
    27301 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
    27361 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
    27421 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
    27481 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
    27541 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
    27601 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
    27661 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
    27721 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
    27781 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
    27841 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
    27901 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
    27961 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
    28021 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
    28081 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
    28141 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
    28201 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    28261 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
    28321 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
    28381 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
    28441 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    28501 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
    28561 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
    28621 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    28681 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
    28741 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
    28801 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    28861 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
    28921 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
    28981 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
    29041 cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca
    29101 caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa
    29161 gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta
    29221 cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta
    29281 gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat
    29341 taccaggcag aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg
    29401 tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca
    29461 tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa
    29521 tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt
    29581 gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg
    29641 aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg
    29701 ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg
    29761 accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa
    29821 gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg
    29881 ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag
    29941 ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg
    30001 aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa
    30061 atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg
    30121 cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga
    30181 ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa
    30241 taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc
    30301 aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga
    30361 tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa
    30421 ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct
    30481 ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca
    30541 attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat
    30601 gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc
    30661 aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc
    30721 gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta
    30781 ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta
    30841 tttttataac tttcacacac aagtttattt tttactagaa agtagcgccc tcttgttttt
    30901 ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca
    30961 acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt
    31021 tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga
    31081 ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac
    31141 cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat
    31201 gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga
    31261 acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca
    31321 ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag
    31381 cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat
    31441 tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg
    31501 cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa
    31561 atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg
    31621 gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt
    31681 tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac
    31741 cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca
    31801 aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa
    31861 ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta
    31921 cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat
    31981 aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt
    32041 ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa
    32101 aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca
    32161 cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt
    32221 ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca
    32281 cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa
    32341 atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac
    32401 gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat
    32461 cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca
    32521 ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt
    32581 ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag
    32641 tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt
    32701 gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt
    32761 aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt
    32821 tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt
    32881 cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa
    32941 tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt
    33001 ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat caataatgta
    33061 gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct
    33121 aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc
    33181 aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata
    33241 ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc
    33301 agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa
    33361 gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa
    33421 agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat
    33481 agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg
    33541 attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc
    33601 gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca
    33661 gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa
    33721 aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt
    33781 ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt
    33841 gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc
    33901 gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc
    33961 aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat
    34021 aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc
    34081 aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat
    34141 ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc
    34201 aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac
    34261 cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag
    34321 ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac
    34381 tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc
    34441 aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct
    34501 ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga caatgacttc
    34561 tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc
    34621 tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac
    34681 tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat
    34741 taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga
    34801 ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
    34861 caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc
    34921 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
    34981 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
    35041 aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac
    35101 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
    35161 tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg
    35221 ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc
    35281 caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag
    35341 gaaaactgaa aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt
    35401 ttttatttaa catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt
    35461 tttccttttc ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc
    35521 ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg
    35581 aactagcttc taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt
    35641 tctccgacac ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat
    35701 ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac
    35761 gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg
    35821 tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc
    35881 tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt
    35941 caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac
    36001 gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct
    36061 atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca
    36121 gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt
    36181 cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta
    36241 attgccagga agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga
    36301 accgacaata attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat
    36361 aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc
    36421 gacaaaccat cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt
    36481 atggaaactt ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt
    36541 aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc
    36601 attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat
    36661 gcaggaaaca tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa
    36721 tggcaggaac gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt
    36781 agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc
    36841 caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac
    36901 agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc
    36961 atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt
    37021 ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg
    37081 atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg
    37141 acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc
    37201 atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc
    37261 aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct
    37321 tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac
    37381 ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc
    37441 aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt
    37501 accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc
    37561 atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat
    37621 agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg
    37681 caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc
    37741 ttgtgtcaat gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat
    37801 ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa
    37861 attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc
    37921 aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg
    37981 agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa
    38041 gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt
    38101 tccaacagaa acaatgattc cttccgcact tccactgatg acattacttc ccataaagac
    38161 aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc
    38221 tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa
    38281 taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac
    38341 gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt
    38401 cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat
    38461 tgtcccgctg acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc
    38521 aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa
    38581 tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata
    38641 tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc
    38701 atttgctgat gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc
    38761 atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga
    38821 cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga
    38881 aaggggctga gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga
    38941 atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca
    39001 atcatttttc gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg
    39061 gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca
    39121 tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc
    39181 agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca
    39241 ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca
    39301 aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt
    39361 ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt
    39421 tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt
    39481 tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag
    39541 catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat
    39601 gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga
    39661 actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt
    39721 gatcgatatc gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac
    39781 gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg
    39841 tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc
    39901 tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa
    39961 tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca
    40021 ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc
    40081 tttgataata atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta
    40141 agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat
    40201 aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg
    40261 ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga
    40321 atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca
    40381 tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata
    40441 cttctgtttt ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct
    40501 taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc
    40561 gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc
    40621 attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg
    40681 acattttttt cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt
    40741 ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat
    40801 catacataat agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt
    40861 aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct
    40921 tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt
    40981 tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat
    41041 tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt
    41101 ccgcttcatt tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat
    41161 aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt
    41221 tttcctgatc tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc
    41281 ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc
    41341 catttcgaaa agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa
    41401 gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc
    41461 caccgtattc atgattgact gtccctttag tataaccagg catatggatc atcaatggca
    41521 ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc
    41581 ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga
    41641 ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat
    41701 ccagataatg tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg
    41761 gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt
    41821 tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt
    41881 tatctaatat cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga
    41941 aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc
    42001 catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa
    42061 agacattgtc tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag
    42121 tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt
    42181 caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc
    42241 cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat
    42301 ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat
    42361 aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc
    42421 ctgttttgat tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg
    42481 aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc
    42541 ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt
    42601 tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga
    42661 tatccagcca gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc
    42721 cttgcgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc
    42781 gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa
    42841 tagctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag
    42901 tcgcaattgg gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa
    42961 aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga
    43021 ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt
    43081 cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta
    43141 agattttttt attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc
    43201 gatgtaagcg attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg
    43261 gtattcaaaa gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg
    43321 tcgcagctcc ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg
    43381 ttctcctgcc aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt
    43441 ttgaaattg
//
LOCUS       DACECB010000017        43207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECB010000017 DACECB010000000
VERSION     DACECB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.017"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..390
                     /locus_tag="IWO04_000998"
     CDS             160..390
                     /locus_tag="IWO04_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0212626.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="IWO04_000999"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="IWO04_000999"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0212627.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="IWO04_001000"
     CDS             1801..1977
                     /locus_tag="IWO04_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0212628.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="IWO04_001001"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="IWO04_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0212629.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="IWO04_001002"
     CDS             2592..3059
                     /locus_tag="IWO04_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212630.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="IWO04_001003"
     CDS             3144..4028
                     /locus_tag="IWO04_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0212631.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4145..4378
                     /locus_tag="IWO04_001004"
     CDS             4145..4378
                     /locus_tag="IWO04_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0212632.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="IWO04_001005"
     tRNA            4466..4546
                     /locus_tag="IWO04_001005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="IWO04_001006"
     tRNA            4553..4624
                     /locus_tag="IWO04_001006"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            5139..5846
                     /locus_tag="IWO04_001007"
     CDS             5139..5846
                     /locus_tag="IWO04_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0212633.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="IWO04_001008"
     CDS             complement(5906..6550)
                     /locus_tag="IWO04_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0212634.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="IWO04_001009"
     CDS             6677..6964
                     /locus_tag="IWO04_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212635.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="IWO04_001010"
     CDS             7072..7392
                     /locus_tag="IWO04_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0212636.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="IWO04_001011"
     CDS             7473..8786
                     /locus_tag="IWO04_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0212637.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="IWO04_001012"
     CDS             8783..9502
                     /locus_tag="IWO04_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0212638.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="IWO04_001013"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="IWO04_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0212639.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="IWO04_001014"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="IWO04_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0212640.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IWO04_001015"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IWO04_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0212641.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO04_001016"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO04_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0212642.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IWO04_001017"
     CDS             14940..15833
                     /locus_tag="IWO04_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212643.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IWO04_001018"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IWO04_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0212644.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IWO04_001019"
     CDS             17770..18627
                     /locus_tag="IWO04_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0212645.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IWO04_001020"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IWO04_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0212646.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IWO04_001021"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IWO04_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0212647.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IWO04_001022"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IWO04_001022"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212648.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IWO04_001023"
     CDS             21383..22141
                     /locus_tag="IWO04_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212649.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IWO04_001024"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IWO04_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0212650.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23053..24087
                     /locus_tag="IWO04_001025"
     CDS             23053..24087
                     /locus_tag="IWO04_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0212651.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            24084..24851
                     /locus_tag="IWO04_001026"
     CDS             24084..24851
                     /locus_tag="IWO04_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0212652.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            24951..26519
                     /gene="liaX"
                     /locus_tag="IWO04_001027"
     CDS             24951..26519
                     /gene="liaX"
                     /locus_tag="IWO04_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0212653.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26698..27003
                     /locus_tag="IWO04_001028"
     CDS             26698..27003
                     /locus_tag="IWO04_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0212654.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            27003..27353
                     /locus_tag="IWO04_001029"
     CDS             27003..27353
                     /locus_tag="IWO04_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0212655.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            27519..28457
                     /locus_tag="IWO04_001030"
     CDS             27519..28457
                     /locus_tag="IWO04_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0212656.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28472..29302
                     /locus_tag="IWO04_001031"
     CDS             28472..29302
                     /locus_tag="IWO04_001031"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0212657.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            29346..30371
                     /locus_tag="IWO04_001032"
     CDS             29346..30371
                     /locus_tag="IWO04_001032"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0212658.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30386..31324
                     /gene="galU"
                     /locus_tag="IWO04_001033"
     CDS             30386..31324
                     /gene="galU"
                     /locus_tag="IWO04_001033"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0212659.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31449..31898
                     /locus_tag="IWO04_001034"
     CDS             31449..31898
                     /locus_tag="IWO04_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0212660.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            31891..32505
                     /locus_tag="IWO04_001035"
     CDS             31891..32505
                     /locus_tag="IWO04_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0212661.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32568..33671)
                     /locus_tag="IWO04_001036"
     CDS             complement(32568..33671)
                     /locus_tag="IWO04_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0212662.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33881..34882
                     /gene="ccpA"
                     /locus_tag="IWO04_001037"
     CDS             33881..34882
                     /gene="ccpA"
                     /locus_tag="IWO04_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0212663.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35236..35631
                     /locus_tag="IWO04_001038"
     CDS             35236..35631
                     /locus_tag="IWO04_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212664.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            35628..36884
                     /locus_tag="IWO04_001039"
     CDS             35628..36884
                     /locus_tag="IWO04_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0212665.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            36896..37219
                     /locus_tag="IWO04_001040"
     CDS             36896..37219
                     /locus_tag="IWO04_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212666.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37212..37478
                     /locus_tag="IWO04_001041"
     CDS             37212..37478
                     /locus_tag="IWO04_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212667.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(37641..39986)
                     /locus_tag="IWO04_001042"
     CDS             complement(37641..39986)
                     /locus_tag="IWO04_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0212668.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            40265..41167
                     /locus_tag="IWO04_001043"
     CDS             40265..41167
                     /locus_tag="IWO04_001043"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0212669.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            41378..43153
                     /locus_tag="IWO04_001044"
     CDS             41378..43153
                     /locus_tag="IWO04_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0212670.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT        14230 a         7536 c         9253 g        12188 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttggggttat atccatacca cgtacaatta atgggtggga tcgtgcttca
    10681 cgatgggaat atccctgaaa tgcgtactgg tgaaggtaaa accttgactg caacgatgcc
    10741 ggtgtatctg aatgcgttga ctggcgaagg ggtccacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga gctttataat ttcttaggat tgacagttgg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gcgatatcac
    10921 ttatagcacg aataatgaat taggatttga ctatttgaga gacaacatgg tcgtttaccg
    10981 tcatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcgatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat caacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tatacaatca aaaacaatca gtttaactga agcagggatc gaaaaagctg aagaaacatt
    11221 cggtttggac aacttatatg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactacatca tgcttttaga catcgattat gttgttcaag atggaaaagt
    11341 attgattgtt gaccaattta cgggtcggat catggatgga cgccgttatt cagatggtct
    11401 tcatcaagcg atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaacgatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggga tgactggtac
    11521 agctaagaca gaagaagaag agttcagaga gatttataat attcaagtat accagattcc
    11581 gacaaaccgt ccggtcatcc gtgacgatcg accagatttg ctttacccaa cattacaaag
    11641 caagttccat gctgtcgtac aagatatcaa agatcgctac cgcaaaggac agcctgtctt
    11701 agtcggaact gttgcagttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtactga atgcgaaaaa tcactttaaa gaagcagaaa tcatcatgaa
    11821 tgcgggtcaa aaaggtgcag tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagctaggg cttggcgtaa gagaacttgg tggcctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacaa ttctacttgt ctttagaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaagct ttcttagatc gaatgaagat cggtgacgaa gatgctgtga tccaaagcaa
    12121 gatgctgacg aaacaagtag aagctgcaca aaaacgcgta gaaggaaaca actacgacac
    12181 acgtaagaac gtcttgcaat acgatgacgt gatgcgtgag caacgtgaag taatctataa
    12241 acagcgtcaa gaagtgatca tggaagaaaa aagcttatct aaaccactga tgaatatggt
    12301 caaacggaca atcagtcgtg tggtagatgc gcatacgcaa ttagaaaaag aaaattggaa
    12361 tttggaaagt atcgtcgatt ttgctggcag cacgcttgtc catgaagaca gtatttccct
    12421 tacagatatc gaaggaaaaa cctctcaaga aatcaaagaa tatctaaacg aacgagcaca
    12481 agaagtattc aataccaaag cagctcaatt gaacggacca gagcaactgc ttgaatttga
    12541 aaaagtcgtg atcttacgtg tcgtggactc taaatggaca gatcatattg atgcaatgga
    12601 ccaattacgt cagtcaatcg gcttgcgtgc ctatggtcaa aacaatccat tagtcgaata
    12661 ccaaacagaa ggttataaga tgttcgaaga tatggtaggt gccattgaat atgaagtcac
    12721 tcgtatcttc atgaaagctg agatccgtca aaacgtacaa cgtgagcaag tttctcaagg
    12781 aacagcttct cactcagaag atggagatgc caaagaaaac agtaatacta gcgcgaaaaa
    12841 acaaccaatc catgtgaaca aggttggtag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcacg gaaaagatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt ttttaaagaa tttgtctcac cttctttctg ttatttgacg attctgttag
    13021 actagatata ataaaaaaag tatagtgaaa gaaggaaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gtaatgcaag aagaagcaga agcacgtatc aatgcgctag
    13381 atgaaaaaat gaatacgtat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt cgaattgcat ccaggggctg gcggaacgga gtcccaagac tggggcagca
    13501 tgcttttacg catgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggcgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ctatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc aaaacgtcgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa gatcgacaca tatcgggcga
    13801 gtggtgcagg tggtcagcac atcaataaga cagaatccgc tgtacgaatc actcacttgc
    13861 ctacaggaac agtagtagcg agtcaggcac aacgttcaca attgaaaaac cgggaacaag
    13921 cgatggggat gctgaaagct aagctttacc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact gagaggagaa caaaaagaaa tcggctgggg atcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagctgttat ggacggagat cttgatttat ttattgacgg ctatttgaaa atgcatctgc
    14161 aataagagcc ctttgttaaa ttgaaataaa attgtaagat tttgcaagca agttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaacgg taccactgct atccgcaatc tctcgattgt
    14341 catcgaacaa ggagagtttg tgtatgttgt tggtccgtct ggagctggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gccatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgccat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggatct
    14641 tgttggtttg aaacacaaag tccgagtctt tcctagcgaa ttatctggtg gggaacaaca
    14701 acgtgtttct attgcccgag caatcgtaaa tacacctaaa gtacttatcg cggatgaacc
    14761 gacagggaat ctagatccgg aaaactcatg ggaaatcatg aaactattag atcggatcaa
    14821 tgcacaagga acaactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 acaccgagta attgctatcg aaaacggccg cattattcga gaccaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagta gcttcggtca gtgcggtaac gatcacgttg actttagttg
    15061 gtgtgttcat ggcggtcatc atgaacgcaa ccaaactagc acaagatata gaaggaaacg
    15121 tggatgtatc tgtgttcgta gacattggta caaaagacga agagatgaaa aaattagaaa
    15181 aagaattaaa tgacttagat catgtcaaaa aagtcgaatt ttcaagcaaa gaacaggaat
    15241 tgaagaaaat ccaaaacgaa atgggcgatt cttggaaatt gtttgaaggg gataataacc
    15301 cgttatatga tgtatacgta gtaagtgcta ccgatccttc ttatacaaaa caagtagcag
    15361 aaaaagcggc agatttgaag aatgtttccc gttctgatta cggtggggct tcctcagatc
    15421 ggattttcca aattgctggt gcagttcgaa catggggatt agttgcggcg gcattgctgc
    15481 tgtttgtagc aatgtttctc atttctaaca cgattcggat cacgatttta tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtagggg caaaaaatgg atatatccgc tggccatttt
    15601 tccttgaagg tggttggatc ggcttaatcg gtgccattct tccgatactg atcattacat
    15661 ttggttacgc atggttttac caattgatca atccgtcatt attacgatct cattattcat
    15721 tgatccatcc agaaaatatc gtttggaaaa tcaatctttt gatggtcggt attggtgtga
    15781 tcattggttc actcggctcg atcatctcta tgagacgttt cttgaaattc tagtgtcaca
    15841 agataaatat aatcagaaag ttggacgatt tcgttccagc tttctttttt tatcagctag
    15901 gtggagtaaa tggggcggct tttttctctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa aaagaggtga agccgtgaag attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatt atcgtttttc gagaaagaaa ggaaactgcg
    16081 caaacatggg catggctgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga atctagaaat cgaagaagat aaacaagcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgcttac tgtatttgaa
    16321 agtacgcttt atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga tcgaggatat caaacaggca gaagaccata tccatttcca atattatatt
    16441 taccgcagtg atacattagg tgaagaagtt cgagaggcat tgacagaggc agcaagacgc
    16501 ggagtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttctagtttt
    16561 tttcagaatt tgaaaaaagc aggtggagaa gtcgcatttt tctttcctct attcgttccg
    16621 tatatcaatc ctcgaatcaa ttatcgcaat catcgtaaaa tcgtagtgat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aacgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tctgcgtatc tatggagaag cggtttacac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggatattc accggaattt
    16861 ttcccgacca ttcattctac aggtgatatg gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg agcaaatcaa aatgacatat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgccgta ttacataccg gatggatcga ttcatgaggc attgaagttg
    17041 gctcttctct caggcgtaaa agtccatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctgatcg agtatggggc aagaatcgaa
    17161 acatatgaaa atggatttat ccatgcaaag acgatgatta tcgatggcgg catcgtttcc
    17221 gttggttcag ctaatatcga cgttcgctca tttagattgg attttgaagt caatacactg
    17281 atctatgatg aacgaatggc ttctcgcgta agaaaagcat tctttgaaga ttcaaagatt
    17341 tcgacgcatc tcacaaaaga agtgtatgaa aaccgaggga tgatgatcaa gatgaaagaa
    17401 ggattagcaa gactgatttc gccgctcctt taaaatacaa aaaggacagg agagccatca
    17461 atagatggaa atctcgcgtc cttttttctc ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagct ttttttgcaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gatagaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact aaaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta ctgcagttgg ctcttctgcc
    17881 ttacagcctt tagtagaaac ggttgctgaa acctaccagg cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact gggttgtctc aagtacagtc aggagcagtc
    18001 gatatcggaa attctgattt gttcgcggaa gaaaaatcag ggattgatgc ttcggcatta
    18061 gtcgatcatc gagtagcagt tgttggaatc actcctattg tgaacaagga tattggcgta
    18121 aaagatatca gtatggaaaa cctgaaaaaa ttatttacgg gcgagatcaa aaactggagt
    18181 gaagtcggtg ggaaagatca gccagtagtt atattgaacc gtgcatcagg aagcgggacc
    18241 cgtagtacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccaaatcgtt gcggacacac ccggagcaat cagctatgtt
    18361 gctttttctt acgtttccga tgatgtggat acgttgaacg ttaacggtgt ggaaccaaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gaccatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataatattg tcggagagct aggctatatc cctgtttcac atatgaaagt tgaacgtgat
    18601 tggcaaggaa acattgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg cggaaaattc attagttttt tgtgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgtc gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatagga
    18961 gcaaatggga aacccatggt aggggcattg cctatgatca caggttcatt tattgtgacc
    19021 tttttatctg ctattgttgc cacaccattt gctattggag cagcagtgtt catgacggag
    19081 atttctccta aaaacggaac gaagatcttg caaccggtca tcgagctact tgtaggtatt
    19141 ccgtctgtcg tttacggatt tatcggttta tctgttatcg tgccttttgt cagaagtatc
    19201 tttggcggga cagggttcgg tatcttagct ggtacatttg tgttattcgt tatgatctta
    19261 ccgactgtca caacgatgac tgtggatgca ttaaaggcag tgccgcgaca ctatcgggaa
    19321 gcttcattag cgctaggtgc cacaagatgg caaacaatct acaaagtggt gctgcgagca
    19381 gcagtgcccg gaattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggcgaagcc
    19441 ttagctatcc aaatggttat tggtaatgcg gcattaatgc caacaggact tactactcct
    19501 gcttctacat tgacgtccat cttgacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ctatatgtgg tttcagggat catcgtattg atcttagcag
    19741 ctttattact ttatatctta gttcgaggaa taccgcacat ttcatggtca ttcttgactg
    19801 aaccttccaa agcctaccaa gaaggcggtg ggatcgggat tcaattattt aactcgattt
    19861 atttgctttt gattaccatg atcatcagtt ttcctatctc tttaggtgcg ggtatctacc
    19921 tttcggaata tgccggaaaa aactggctga cagacgtgat ccgtacgtct atcgaaatct
    19981 tgagctcttt gccttctgtt gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcaatc atttctggtg cattagcttt gaccgtattc aacttgcctc
    20101 ttttgactcg aacggtcgag gaatctttac aagcggtcca ttacacacaa cgagaagctg
    20161 gattggcgtt aggtttgtca cgctgggaaa cagtgataaa agtggttgtt ccagaagcga
    20221 caccaggaat tttgactggt gtgatcctaa gttcaggaag aatttttggt gaagctgccg
    20281 cattgattta tacagctggt caaagcgcgc cagcattaga ttttacgaac tggaatccat
    20341 taagtgtttc tagtccgatc agcattttcc gccaagctga aacactggct gtccatattt
    20401 ggaaaatcaa cacagaaggt acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat cattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagtaaa atgaaaaaat ataattggaa
    20581 tgaaacaaat gtcattacca tgccaaaaga taaggaagtt gcattatata cgaatgatct
    20641 gcatgtttgg tatggcgaca atgaagcaat caagggagtg gatctagaat tcgaaaaaaa
    20701 taagatcact gcattgattg gtccctctgg ctgcggaaaa tccacgtatc ttcgttcttt
    20761 gaatcgtatg aatgatggga ttgctaatac gaaaattact ggaaagatta tgtataaaga
    20821 cgtggatatc aatgcgcctc aagtggatgt ttatgaaatg cgtaaacgga tcggcatggt
    20881 gttccaacgc cccaaccctt ttagcaaatc gatttatgac aatataactt ttgctttgaa
    20941 acagcatggt gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agcagctttg tgggatcaag taaaagatag cttggataaa agtgccttgg ctttatctgg
    21061 cggacaacag caacgtttgt gtatcgctcg tgcaatcgcg atgaaacccg atatcttgct
    21121 gttagacgag ccggcaagtg ccttggatcc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact acacgatcat tatcgtgacg cataatatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tctaggaaaa gtaatggaat atgacaaaac
    21301 aaacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agaaattatc tcatccaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaggg aattgatcta agtttcaatc aaggtgaaat
    21481 cacagcgatg atcggtcctt ctggttgcgg gaaatctacc tatctacgtt cgttgaatcg
    21541 aatgaacgat ctgattccaa atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccgaaaacag atgttgttga actaagaaaa gaaatcggca tggtgttcca
    21661 acagccaaat ccatttcctt tttctattta tgaaaatgtc atctatggat tgaagttaaa
    21721 aggtgaaaaa gataaaaaag tccttgatca agtagtggaa gaaagtctga aggcagcgtc
    21781 cgtttgggag gatgtgaaag ataaactgca taaaagtgcc ttgtctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagtgaat cccgaaatca tcttattgga
    21901 cgaaccaaca agtgcacttg accctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaacg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgctgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag ctcaagaagt tatcgaaaac gatataaata tcaataatat
    22321 ggaaacgcgt ttggagaaga aaagttttga aatgattgca ttacaacagc cagttacaac
    22381 tgacttgcgt atgattatca cggtcatgaa agctagctct gatttagaac gtatggccga
    22441 tcatgcggtg tccgttgcta aatccacaat ccgcgtaaaa ggacagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttacgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcgtat gtcaaaacag acgaaaaaga tgcgcgtaca atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaaccatc aaaaacatgc aagaaaatcc
    22681 tgaaacagtc atcagtggca cagactattt gaatgttgcc agctatctag aacgaatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 attaaatacg aaccacaatg agattttcta aactgaagtt gtgattctgc tgtttattta
    22861 ggaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggtaaaat
    22921 agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    22981 ttattctgga aaagggtttg tgacaaaaca tgtcacaaac ccttttcatt attccaatag
    23041 aaaggataaa gaatgcaaaa aaagattagc atcatcatca ctctattttt tatttgtata
    23101 cttgcttcat ggtttattca gcgtcaagct gctctatatg acgtacctgt catcgagatc
    23161 gaaagagtga gaatagaaga ggatcaacaa ttgattacag gaagattgat gaatacggac
    23221 caaaaaggag aagtgatttc tttaacggct ccttatcaaa aaaatcaaat cgaaacagca
    23281 aatataacaa caagacaaat ctatcttgtc caaatgcatg gagatgaatg gcaactagta
    23341 gcaaaaaaaa atgatggctg gctttttttc tttacaagtg tcttcattgg caccatgttg
    23401 cttattggtg gaaaacaggg cgtatttgct ttaggaagtc tcatcatcaa catcgtttta
    23461 cttttacttt tgttgctcct ctttaccaaa acagaaggta tttctctctt aaatatagca
    23521 atcttatttg tcgttatagg gataatcgtt agtatattgg cgttagacgg atggaataga
    23581 agcagtataa tcaaaatttc ggctgcagtt acaagtgttt ttttagcatt ttttatttgt
    23641 cttttgacaa tgaatcattg gaaagatcaa ggactgcgct atgaagaact gaattatctg
    23701 acacgaccgt atcgttctgt ttttctttcc agcgtgctga ttgggacagc tggagggaca
    23761 ctcgataccg tgatcacagt cattgcgaca ttagaagaat tgaccaagca acaaccgaaa
    23821 atatcagcga aacagttatg gctttcagga aggcaagtgg gccgagatgt gattggttcg
    23881 atggctaata tccttttatt cgtctatatc agcggagcga ttccatcgct tctgttatat
    23941 ttaggaaatc aatggtcttt caaagaaacg gtagaacgac atttatcatt agaactttta
    24001 cgcgtcattg ctggcagctt aggaatagta ttatcaatcc ccatcgcggt cctctttttt
    24061 ctcgctgtaa ggaggttaaa aaaatgaatg tacttattat acttgttggg atttttgcaa
    24121 tcagtgtgct atttgtcggt ggtacgcaag gtatgtatat tcttttagga ttattcatta
    24181 atttaggaat ctttttccta ttattattcg gctatcatca gaagtggccg atattggtat
    24241 tgtcgattat agggttccta ttgatcgctg tggttatctt attttttatc aatggttata
    24301 atctgaaaat gcgagccgct tttgccagta tactgatctt tttgttttgt ttcctactat
    24361 tgattcctat cactgatttt ttagcgatcc aaggctttac ttcgatagaa ctagaagaac
    24421 tatctggctt agataagacg ttagcaattg attttagact acttacgagg tcgttgcttc
    24481 tgatcagtct tagcggagct gttttagatg cgagtgtagc gatttctagt ggaacattcg
    24541 aagtatatca ggcaaatccg caaatgtctt ttaaccaatt gcgccatgct agctttgcca
    24601 ttgctaaaaa aatattggca tcaacagtga tgacgcttct ttttgcgttt atggggagtt
    24661 ctttagcgtt gattctttgg tttattgact tagacatttc ttttcagcaa ataatcaatg
    24721 aaaaatcatt tgtcttggaa tatacgatgg ctattcttac cacactggcc gcactacttg
    24781 tcctgccctt aacttgcgtg actacagcat atctctttac aaaaaagaaa gaacatctca
    24841 agatggagta aaagttacaa cttaagtcct atgacatttt tactgaatca gtgcataata
    24901 tggatgtaag gaaaagcttg caaacaattt tgcttaagga ggcaatttcc atgaaagaaa
    24961 gagaacgtat tttagactta gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt
    25021 tattagaaag catggcaact gaaaaagacg aaaaacaaat caaaaaagaa gcagacaagg
    25081 taactgctag tcataaagaa aaagatcagg caagtcaatt gatcgatgaa cttgaaaacg
    25141 gtgaagaaaa aatttctgag ccagttgatc caaaagaaaa agaaagacaa gatcaagaaa
    25201 acttagaaaa gatcttagat gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag
    25261 acgaagtaaa tgctcaattc gctgacaata aggcagcacg taatgagaaa caggaagcat
    25321 tgatgcagct gaatacaaaa gaagaattag gcgagttgac agaagaagaa ttagcacagc
    25381 gtcaaacgtt agaagcagaa atcaaagagt tagaagcaac cggcgataca ctattagaag
    25441 aacaattgaa gctggaagca gaattaaaag atatccgcaa gaatcaatgg tctgagaaaa
    25501 aagaaacatt tacggataag tttgaattgc cagatgattg gaaagatcaa gcgacagata
    25561 cgctgaatca agtaggcgaa aaaatgtcag aagctggatc acagttaggc aaattcttga
    25621 agaaaacatt ccaaactgtt tcagaaacag taaatgataa catggaatgg aaagatgtca
    25681 gcttacgcgt ccctggaatt gcgacaacga aatttgaaca cgaattttat tatgaagctc
    25741 ctgcagcaag tatccttgat atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg
    25801 acagtgatga tgtgaaagtg gaagccaaga tcaaacttta cggaaaaatg ggtgctgaac
    25861 catttgaagc attctctgaa cggagccaga tcgaggtcaa tgaagatcat atctccttcc
    25921 aaattccaaa taagcgtgtg cgtgcagacc ttgttttcta cttgcctaaa cgagtatacg
    25981 accatgcagc tatcaaactt ctaaatggca acatcatgat tgagacattg gaagctaaag
    26041 atatttatac aaaatctacg aatgggaaca tcatcgtgaa ccaattaact gcaacgatgc
    26101 tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa cgggaacatc ttagattcta
    26161 ttatagaaac agtaaacggt acagtgacat ttggtgccac accagaaaac ctttctgtgt
    26221 ctttagtaaa tggtgatgta cgtcttacga ttaaagaaga taatttgaaa aaagtagaag
    26281 caagttcagt gaacgggaat gtcaaagttg ctttaccaga tagcatcggt ttggaaggac
    26341 acgctaaaac aagtcttgga agcattaaca gccgtctgtc caattacgaa gtagttcgtg
    26401 agaaaaaaga acggacaaac caaatgttgc aattccgtcg tgtttcagat gatgagatcg
    26461 cacaagtgca gctttcaacg acaacaggaa gtatctattt aaaagataca gataaataaa
    26521 gaggttgtga aagagatgtt cagccttgag caataagaag cgatttttta aaatagcttc
    26581 ccctatttta aaaaaatgtg ccttattgcc gataggctat ctcttgaaca ccgattaaga
    26641 agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg agacatgatg
    26701 aaaaaaaagt tgacaaaatc caataaaaat gttgttttga caggtacttt agcagggatt
    26761 ggggaatata tcgggattga tcccactgtt atccgtgtgc tttatgtatt cttaagctta
    26821 gttgcttttg gaagtccaat cattttatac attttattag ctttgattat cccatctgcg
    26881 cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa caattatcgt
    26941 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
    27001 taatgtcgta ctttcagcgt attttggtta atacgttaac attcatatcg ttggctgttc
    27061 tttttcctgc gatggtccat gtgagcagta tttggacggc cgtccttgcc agcttcgtat
    27121 tatctgtgtt gaacatgttt gtcaaaccga ttttgacgat cctgtccctg ccctttacat
    27181 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacttcct
    27241 tttttgtagg agcagaacat tttagttttt cttcatttgg tgcagcgtta ttcgtagcta
    27301 tcttgatgtc acttgtcaat gtaattgtta gtgaacataa cagcagaaaa taaaaaaagc
    27361 tgtgccgaga tctcggcgca gcttttttgt ttagaataaa gaatgcgttt tctattacat
    27421 tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa
    27481 atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta
    27541 gccaattagt aaaaaaatta tctttggaaa tcgtcacagg cgacgagaaa agtttagacc
    27601 gcgtcatcgt gactggggac atctcacgcc caggactaga gttgaccggt tattttaatt
    27661 attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga
    27721 tgatcccaga agagcgtttg atcattatga gaagattatg tagtgaagat atgcccgcat
    27781 ttattatttc aagaggattg ccggctccgg aagaaatgat ccaagcagcc aatgaaaatg
    27841 ggatagctgt gctgcgttcg cctatctcaa cgtcccggct gctaggtgaa ttatctagtt
    27901 atttagatgg acgcttagcc cctcgaacaa gtgtccacgg tgtgttagtc gatgtctacg
    27961 gattaggtgt actaattcaa ggggacagtg ggatagggaa aagtgaaacg gcgttggaac
    28021 tgattaagcg cggacatcga ttgatcgcag acgatcgagt agatgtctat cagcaagatg
    28081 aactgacggt aatcggagaa cctcctaaaa tcctggaaca tctgattgag attcgaggga
    28141 tcggtatcat agatgtaatg aatcttttcg gtgcgagtgc ggtacgaggc tctatgcaag
    28201 tccagttagc tgtttatcta gaagcatggg cgaaagataa gaaatacgac cgtctgggct
    28261 ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca
    28321 aaacaggacg aaacgttgca atcatcattg aagtagccgc aatgaatttc cgcgcacgta
    28381 caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa
    28441 actcaggcaa tgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt
    28501 tgagcttgta ggaattccta tctactggta tgcattgatt atcgtatcag gaatcattat
    28561 tgccatgtgg ctcagcagca gagaagctgt tcgggtagga ttaaaagcag atgacgtgac
    28621 agattttatg ttgtggggat tgccgatttc cattattggt gcccgcttgt attatgtttt
    28681 attcgatctt ccccaatata tagctgatcc aatccagata ttcaatatcc gttcaggagg
    28741 attagcgatt tacgggggat tgattgcagg tgcaatcaca ctgtactttt ttaccaaata
    28801 tcattttatt tctttgtgga cctttttaga tatcgctgca ccaagcgtat tattggcgca
    28861 ggcaatcgga cgatggggaa atttcatgaa ccacgaagcg tatggtccag acacgacacg
    28921 aagtttctta gaaagtctgc atttaccgca attcatcatt gataacatgt atatcgaagg
    28981 aatctatcga caaccaacct ttctttatga atcactttgg agtttgattg gatttattct
    29041 gcttttggtt ttgcggaaga aaccacatct tttgaaaaaa ggagaagtcc ttttatgtta
    29101 tctaatgtgg tatagtttcg gacgtttctt tatcgaaggg ttacgcacag acagccttta
    29161 ctttttaggt gtgatccgca tttctcaatt attgtctgct gttttatttg tcggcacatt
    29221 gattctgttt atttggcgta gaaaaaaagg aagtttaccg gattatgacc gctcattagg
    29281 aaaaaatcaa caaattattt gattttcaga gcgttaaaaa tgaaaaaaat ttcaggagtg
    29341 gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttgggggact gctttagcac
    29401 aagtcctcgc tgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag
    29461 aagaaatcaa cacccaacac accaatcaac gttatttacc agatcatcat ctacccgatt
    29521 ccatccgagc attcaccgat atggaagatg cagtaaaaga tgcagatgcg gtactattcg
    29581 ttatcccgac gaaagcgata cgttctgttg caaaagaact agttgccaaa ttgaatacga
    29641 agccagtgat catccacgca agtaagggat tagaacaggg aacgcataaa agaatctctg
    29701 aaatcttgtt agaggaaatc ccgacagaaa aaagaaaagc tgttgctgtt ctttctgggc
    29761 caagccatgc ggaagaagta gcaaagcatg acattacgac gatcacagcg gcaagcacaa
    29821 atgtaaaagc agcaaaatat gtacaagagc tgttcatgaa ccattatttt cgaatctata
    29881 ccaaccctga tgtgattggt gtagagatgg gagcagcttt gaaaaatatt attgccattg
    29941 gagctggtgc tattcacggc ttagggtttg gcgataatgc aaaagcggca atcatgacgc
    30001 gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta
    30061 ttggcttaag cggagtaggt gatctagtcg ttacttgcac aagtgttcat tccagaaact
    30121 ggcgcgcagg caacttgtta ggacaaggtc agcctctaga agaggtgcta gccaatatgg
    30181 gaatggttgt tgaaggtgtg aatactactc aagcagcgat ggaactagcc caaatgatgg
    30241 aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca
    30301 aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa ttctcattaa
    30361 caattttttg aaggagcgaa tgtatatgaa agtaaaaaaa gcagttattc cagcagcagg
    30421 attaggaaca agatttttac cagcaacaaa ggcgatggca aaggaaatgt tgccaatcgt
    30481 ggacaaacca acgatccaat tcatcgttga agaagcgttg aaatcaggaa tcgaagacat
    30541 cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatatcga
    30601 attagaaaca aacctaagtg aaaaaggaaa aaccgaatta ttgaaattag tagaagaaac
    30661 gacagatgtc aatttgcatt tcatccgtca atctcatcca aaggggttag gacacgccgt
    30721 tcttcaagca agagccttta ttggaaatga accgtttgtc gttatgctag gcgatgatat
    30781 catggaagac gaagtaccat tgacacagca attgatcgat gactatgagc gtacacacgc
    30841 ttctacgatt gccgttatgc aagttcctca cgatgaaact tcaaaatacg gaatcatcaa
    30901 cccaggggaa gtactggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga
    30961 tccaagcaaa gcaccaagtg atttagctat tattgggcgt tacttattga ctccagaaat
    31021 ctttgatgta ttatcagaac aacaaccagg cgctggaaat gagatccaac taactgacgc
    31081 aatcgatacg ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta
    31141 tgacgttggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc tgacacatcc
    31201 agaagtaggg gcacctttgc gtgaatacat catgtcttta ggtaaaacat tagaagcaga
    31261 aggaagcaag atcaatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa
    31321 ataatctttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt
    31381 ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga
    31441 gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc tttgcgatac
    31501 ttgtcgtttt catcgttcgc gtattgttgc aagtttccaa aactttagaa aaagtagacc
    31561 agactgtcgc agaagcaaat acgacaattg agatcgtcac acaagacgta gatcttttat
    31621 ctagacaagt agaaggcttg ctagtcaaaa gtaatgagct gctcttggat atcaatggaa
    31681 aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcgg
    31741 aacttaatca ttctagtaaa aacatcgtga cgaaagtcgg cggaatcgga aaaacgacag
    31801 ctaaagcgac tgttgccgga aaagtcggtg gaacagcgat gaaattttta aaaacaaaaa
    31861 acatactgaa acgacaggag gaaaataaga atggctaaaa aaggtggatt tttattaggt
    31921 gcagtagttg gaggtacagc cgcagcaatc gccgcattat tgttagcacc caaatctggt
    31981 aaagaacttc gggatgaatt ggctgctcaa gcagatgatt tgatggacat ggcttcagat
    32041 tacacagata tggcaaaaga aaaatcatct gagttaacag gtattgcaaa agaaaaagcg
    32101 caagatcttt ctcaacaagc aggggatcta gcaggcaatg ttaagcagaa ggtaagtgaa
    32161 tcagcagagg aagcaggaag cgaatcagat gatatcttgt taaacctcaa agaacaatca
    32221 gctgatttat ctaatcgttt caaaaaaaca gcagaagatc tgaaaaagcc agcagacgaa
    32281 ttaggtagta ttgctaaaga tacttcagaa gatatcatga tcgatgtaaa agatgctgca
    32341 gataaagcag gagagacggt caatgaagga atgtcagaag caaaaccagt tgcagatgac
    32401 acaaaagaaa tgttgaaaat ggcaaaagaa gatgcaaaag aagatgcaaa agaagctaaa
    32461 aaagaaatga aagatacaat gacagattcg actaccaatc actaagatta ctcatttcta
    32521 gaataaaaaa ggtgagagcg gaccagctct cacctttttt attttcatta ctctaaaaca
    32581 agtaattctt ttggtgtagt cgtgaatggc acacaaccat cacttgtcac atacacacaa
    32641 tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac
    32701 atcccttctt caatcactaa gtcgtttcct tctacaagag aagggaattc gtggacggtt
    32761 gttccgattc cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc
    32821 acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctgggcgaac tgcctcttgt
    32881 gcggcaagct gtgcttcaag agtgatgtta tagatttttt cttggaattc agttggtttt
    32941 tgataagcaa ctgtacgagt agcatcactg caatagccat tccaaataac gccaagatcg
    33001 aataacacta gttcatttgg tgaaaccttc gtgcttcctg gtgtaccatg agggcttgca
    33061 gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttt
    33121 aattggtatt cgatttctgc gataattgct tgttcagtta cgccttcttt tactgttttg
    33181 aagccaattt caaaggcaac atcggcccat ttaccagctt cgatcagttt gtctttttct
    33241 tctaccgttt tgatcagttg cagacgctgg ataatagaag ttagatcggt gtaagaatct
    33301 gtacctgtca agatatcaga gatagcatcg aagcgatcga cagtcagagc attcttttca
    33361 atacctacat taccaggttg cccatagcgt gcgcgaagct cttttccgat gatctcccaa
    33421 ggattttcgc tgtctaagta gccacgtaca tcgtacgccc agctgctgtt ttgagcatct
    33481 tcaacttcca gagcaggagt gaaaaggaaa ggatcttgtt ccagagggat aaaaagagct
    33541 agtacacgct cgtgaggatc actttcatat ccggaaaagt aagcgatgtg gccagggtcg
    33601 ctgatataag ccaattctaa ttggtgttgt gccatccatt gacgtagttc attgattttt
    33661 acgttattca tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact
    33721 gattgattaa aagaccaacg ttcttaaaac tgaaaaaagt aaaaattttc atgaaaacag
    33781 agataaaacg gttgcaattg ttgagattat ttgatatcct attttagaaa tgtaagacgt
    33841 attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac
    33901 tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa
    33961 tggcaatcca aacgtcaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt
    34021 ggattatcgt ccaaatgcag tcgcacgtgg cttggcaagt aagaaaacaa caacagttgg
    34081 ggtaatcatt cctgatgtca gcaatatgtt ctttgcctct ttagcacgtg gtatcgacga
    34141 tgtggcaaca atgtacaaat acaatatcat cttagcaaac tcagatggca acgatcaaaa
    34201 agaagtcaat gtattgaata atttattggc taagcaagtg gacggcgtga tctttatggg
    34261 tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaacgc ctgttgtact
    34321 agctggttcc attgatccag atgaacaagt aggtagcgta aacattgatt atgtatcagc
    34381 aacaaaagat gcagtaaata aattagcaaa aagcggcaat aaaaaaatcg cttttgtcag
    34441 cggtgcgctg attgatccta tcaatggtca aaaccgtcta aaaggctata aagaagcatt
    34501 aagcgaaaac aatctaccat atagcgaagg attgattttt gaggcacaat acaactttaa
    34561 agatggtctg tctctagttg accggatcca taatagcggt gcaacagctg tctatgtttc
    34621 agacgatgaa ttagccattg gtatcttgga tggtttatta gaccgcggcg taaaagtacc
    34681 agaagaattc gaaatcatca caagcaacaa ttctttactg acagaagtag ctcgtcctcg
    34741 tttgacaagt atcacacaac cattatacga tttaggagcg gtatccatgc gcctattgac
    34801 gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga
    34861 agaaaaagga tcaactaaat aataaaagat caatcattta aaggttgttc ccaaagtggt
    34921 caacttctgg aacaaccttt atacgagaaa aggggttatg acaaaatttt gtcataaccc
    34981 ctttttttgt gactttaaaa aaaagagaga atggcggaaa caaaccttac gtccatcgta
    35041 tacgacacaa tctgtgcgta gatgctacat tttgttcact actatcaaaa ttactttaaa
    35101 atgaaaacgt gataaaaaag aaagtagaaa agtagaaaag taactctaaa aaatccaaaa
    35161 aaatccaggc tcgattgcgt acttatctta aaggagaaac aatccatcat acataacccg
    35221 aaaggagact tctatttgcc taagaaaaga cgaaagacca tagtcatcat tttgaccatt
    35281 cttactatag gaatcggagg aaaattttac atggacaaaa gagaagcgca aaaacagcaa
    35341 gatttactcg cagtagaaaa acagtccgtc aaagttctga aaaatacctt tgcggatatc
    35401 aaagaagtga aaatcgaaca atttgagaaa aacgacatga caggaatcta tgaagcattg
    35461 ataacgatga ccaatacaga aggaaaagcc gtttattttg attatagctt tacaaaagaa
    35521 agcaatgaga tagatggcta tggtgttgaa aatgaagaag tacaaaaaga agggattacg
    35581 gttgatgaga ttatagttat ctatacagat gggagtgagg aaaaagtatg acaattactg
    35641 ataagcagta taaaggaata agtaataaag tatattggct agatccaaaa catcctcaat
    35701 atagtaaaag ttacaaagaa ggcgccatta aaaactttgg agaaacaaaa ttccaaatcc
    35761 taaaaatcaa aaactccttc gacggcatgc aggcgatggc tgtagcgcca atcaagaacg
    35821 gtaaagtgga tatttctcaa gtagtgattg cttttgcagg aacgaactta agcgattgga
    35881 aagatttgga aacggatgca agaagtgtta tgaatctcta tgggaataga ccaggagcaa
    35941 gtttttctga aaaagcaaca acaaacaaga ttttatccgt tagtggattg aaaacagcgg
    36001 ggcaaatcaa ttcagcgatt gcatttgcta atgaagtaaa aaaggaatat ggagaagctg
    36061 ctatttctac taccggccat tcattaggtg gattcttagc attatatgta gcagcagaga
    36121 aacaatggaa aaatgtcggc ttcaacgggc cggacccgta tgatctctta tcaccagaag
    36181 caaaaaaatg ggtggaagaa aatcccggta tgctcaccaa ttaccgaaat cacgccgatt
    36241 ttatcggtaa ttttggtggt aatggcacag gtgctgaagt tcggatcagt atggaaacag
    36301 catacttgaa tctgcttgct tcccacagtt taacctcttg gaagtttgat gaaaatggaa
    36361 agctgattat tcctgaaaat gaaaacaatg ttaaagctcg aaaacaacag aaagaaaacc
    36421 aagcgaccca gttgttttcc tatcagttgc aagaactgaa caacatacgt aaaaaactac
    36481 gattaagtgg cggccggctg acagttaatg aacaaattta cttagatgac acacaagcta
    36541 ggttagtggt ggaaaagtta tcggtaaaaa tgaaaagcgc tatggaagaa gttatccgca
    36601 tcaaccaaag aggaattttt gagctagaga aaaaatggca agaaggcttg cggcaagcac
    36661 gtgttgtcgc cccttcgttg actgaacagg aaatcataga agcattagct agtgtgggcg
    36721 ccacaaaaga gaacttagtc gattctgcga aagaagtcta tctgctacga atcaataagg
    36781 caagacgtat gggggaggcc tttgatacat tggcgaagga gatccaagac aaaatcaatg
    36841 aaatcgtggc acgagatcga gaattagcac agcaattcaa ttaaaggaga agtagatgaa
    36901 actagaagaa ttggaaagag attatcgaag tgaacgaaga caattagaag ctcaagaaga
    36961 tgaattgatc cactttaaga atcaaggatt acgattgatg gagcaagagg agcagcgact
    37021 gcatcatgta ttaaagaact tagctttaga taatgagcct ctgtttcaag caagacgaag
    37081 ttattcgaat tatcaggagc attatcaaca agccctacaa gtagaacgac gaaaactcta
    37141 tcagcagcaa gacgaactag aacgttccta tcgtcaaaaa atcagacaaa taactatgga
    37201 gaaatcatct tatgaatgaa tttttaatgc aattactaaa agaattagag aaaaatctac
    37261 atcgatcaaa tgtacgttca gttggtatgg ggatatctca gcaaacttgg gcagaaaaac
    37321 gattgacaga tcaaaaccac caaatgactg cagaagaagc tactcaagaa ttttctaacc
    37381 gacatgcaca cttaaaagaa tcagtaaaag ggcaatttgc agaaaaacta accgaattat
    37441 tccaaagaca agaaaagaaa ttaaaacagt ttcgttagta aagttatata gaaaaatttt
    37501 gtgaaaatgg tttttcacat ttttacataa atttgtctta atatcgtaga tgtaagcttt
    37561 tgaacaccat ttattcggaa aaagggagct gtgacaagat ttttgtcgca gctcccttta
    37621 attctgatta atctattaga tcaatcatct ttatcgtctg attctttggt ctctggattc
    37681 gctctagcat aagctgaagc ggtgttccag taatctgtat agttttcatc agtaccgcca
    37741 acgccgaatt taaaggcgct tttcttcgga ccattttttg cccaaagtga ttccactgtt
    37801 tttcctggcg tttggataga ttttttattg acagtaactt ttccagctgt ttgtcctgtg
    37861 aacccagata cttttttctt catcacatcg gatgataagt caaatttctc atcagtgccg
    37921 aaaatctcag ggtttgcttg gtaagcttga ttgattagat tggcaagata attagccgaa
    37981 cgtattcttg cttggctgtc cattccagta ttgtcatcgt gtcctgccca actactgata
    38041 gtgatagatg gagttgagac gatcagccat gaatctcggt attcattggt agaaccagtt
    38101 tttccgaccc aatctgcatt tcctagattt ccgtttaagc ttgagatgac atctttgaat
    38161 ggtgtagtga ttttttcatt gatcacacta cgcatcatgt cattcatgat cgatgccgtt
    38221 gcttcggaat aaatacggac agaattactt tcatgtttat aaatcacatt acctgcatta
    38281 tctgtgatgg aatcaatgat atagccttct tcgtagactc caccatttgc taaggcctgg
    38341 aatccatttg tctgttgaag cgtcgttaca tctactgtcc ctaaaggagc cgattcaacg
    38401 ccccaattat catttgctgg gtagttcatc ttcgccaagt agtgctcgta agcgtattgt
    38461 ttcgatccac cggaattcaa cacatcttga tataagtgat aagctgggat attgttggac
    38521 cattccagtg actcacggac ggtttggaag gtgttcgacc cttcattggt cgcattgacg
    38581 attttctcac cagcattttt tccttcttgc caagtcgttg cataatctga tacacgggat
    38641 tctgatccga caagtcccat atcgattgca ggaccgtaaa caagaaccgg tttgatggat
    38701 gaaccagctt gacgttctgt atcaaaggcg tggttatttt gattctggct gtaattccga
    38761 ccacccacga agccatagat acgtccggtt ctgttatcca tcaatacatt tccagtctcc
    38821 acttggctac cagcaccatc atctaacatg tagccataat tttctacgcc ggtttgcaat
    38881 gcagcataga tatctttatc aatcgtcgaa tggatcgtat atcctttgtt ttggatcgtt
    38941 tgctgtgctt tttgataata agcttcactg accgatgaat ctgacatatc agcattatct
    39001 ttttctgcca attgttgtgc aataatcctt gttgcttcgt tcatcacggt ataatacaag
    39061 aattctctgt cagtctgttc cgcgatttgt tgtgggagaa agtcttttgt aagatcatag
    39121 gctttcgctt cttcgtattg ttctttcgta atttgtcctt cacggtacat actgaataaa
    39181 acaaaatctt ttctggcaag accggcagaa agatcttctt ttagcgcgcc tgtattcgtg
    39241 tatggactat acgtgatagg gctttgaggc aaaccagcga tataagcagc ttgcggcaga
    39301 gtcacatcct ttgcatctac accaaagatt cctctagcag cttcctggac tccagcaata
    39361 ttttgcccct tattattccg accaaatggt gagacattca aatacgtagt aatgatctca
    39421 tcttttgaaa agtgtttttc tacttgtgca gaaagaacga tctcgtttgc tttacgtttg
    39481 aatgttgttt catcggttag gatctgttgt ttgactaatt gctgtgtcaa agtggatcct
    39541 ccagacgagc cgatgccagt cgcttctgaa actagtgctc gtaaaacagc tttagggaca
    39601 taaccttggt gtttatcgaa gtattcatct tctgtactga taatcgcggt tttcaataaa
    39661 ggcgaaatct tatcagatga aatagtcgtc cgcatcagat ctgtctggat ttttgatatc
    39721 tctgtattat ctgcataaac aatctttgac gtttccgtaa tgtttcctag ttctgtctgc
    39781 agttcttttt tagtcggcaa ttgtgtgtct tcgacaagat aggcaaagta gccagcacct
    39841 attccgatac ctagagctgc gaacaacaaa agaagaacag taataaaaac agtcaatgat
    39901 tgaaacactc taaaaataat cttaggaaca aaccattttt ctttttttct tctattctgt
    39961 ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt tatttcgcta
    40021 attatagcaa ataataggac aaaaaaacag acaaggctgt ttttgacagg aatttctttt
    40081 tcgaatgaga taaaacgtga ggaaatgatc ttttttatga aagagttctt aagcttttct
    40141 ttagggaaag tttcagttac tatggagaac agggcaaaga aaaacaaact attttctttg
    40201 tacatcatct tatcaatata caggaattgt tcgaaacaat tgcctttcaa ggaggaatcc
    40261 atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt taggcgcggc
    40321 agaagaagca gccagttacg gatcttcgac ctttatgatc tatacaggcg ctcctcaaaa
    40381 tactagaaga aaagctatcg aagagatgaa tattgatgcc ggaaaagcat ttatggcaga
    40441 aaacggacta acaaatatcg tcgttcatgc gccttatatc attaatttgg gaaatacgat
    40501 caaaccagaa atgtttccat ttgctgtaca atttctgaga gaagagattg tccgtgcgga
    40561 agctttaggt gctactcaaa tcacccttca cccaggcgct catgtaggag caggggcaga
    40621 agctgggatt gctagaatta ttgaggggtt gaatgaagtt ttgacaaaag aacagacggc
    40681 acagatcgct ttggagacga tggcagggaa aggtacggaa atcggtcgaa catttgagga
    40741 attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta cttttgatac
    40801 atgtcatacg aatgatgccg gatataatat aaaagaagat ttttcaggtg taatggaaga
    40861 gtttgataag gtcatcggat tagatcggct taaagtactg catatcaatg attctaaaaa
    40921 tcctcaaggt tctcataaag atcggcatgc caatatcggt tttggaacaa ttggatttga
    40981 cacattgaac aaaatcgtcc atgatccaat gtttgacaac atttcgaaaa tattagaaac
    41041 cccatatgtc ggtaaagata aaaagaaagc ttacgcacca tatggaaaag aaatttccat
    41101 gttcaaagcg caagcattcg atcccgaagt gttccctgag ttattagcag aagaagtcac
    41161 actttaagtt ttctacctga cagcaacaaa aaaggattgg gcagtctgcc ttgtcctttt
    41221 ttgtttaaga aacaaaggag tgctttttgc attcgttttc gccatgctcc ttttgccccc
    41281 tctttttaaa ttcagacaaa cagggtatac tgatagaaaa gtaaaaaacg aaaagtttca
    41341 caaacaagta gcaaaacgtg attgaaagga cgaaaagatg ctgaacattt taaacccctt
    41401 gatcgaccaa ctaaaaaaga aacatacata ctttattctt ggcatagccg tattatttac
    41461 aattattcgg ttgtatttat atagtaaagc aatctatgga acagatatgt cggcgaccta
    41521 tgatgatcag ctgctgattc actatgctca aaatattttg gatggtaaat ggctagggga
    41581 gtacacgact acgacacttt caaaagggat ttcctacagc ttgtttttag tacttgcaca
    41641 taaactgcat ctttcttatg cagttttatt cggcatgttc agtattcttg ccagtatatt
    41701 ggttgcacta gcgtttaaac caatcgtgaa gaacaagtat cttcttttaa gtatctattt
    41761 ttattttttg ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa
    41821 tgctctcgtg atcccgactg ttgagatcat tatcgcttgt ttactagcta tttattaccg
    41881 caaatataat tccagcaaaa aattgctgcc gtggttttta gggctagctg tttcttttcc
    41941 tttcttttgg ttcattcgcg aagattccat ctggcttctt ccttttactg tagtcgcgtt
    42001 aggcatcagt ttcgttcaga tcttgctagg acaagacttt tctttcaaag aagcaaagaa
    42061 gttaaaagaa cgaccatttc tcctttcgaa acaagaatgg ctgaaacttt cattgttttt
    42121 actccctcta gtcggtttct ccatcgtcta tcagtggatc gaatataaaa acgagacaac
    42181 atatggtatt gcagccatca atgaccgttc gagtggcgaa tttggacgtt tgatgaaaca
    42241 gctgatccga atagatgacg aaacaaactt aaataaagaa aacagtgaga tatgggtctc
    42301 gcatgctgca ttaaataaag ctttagacgc atctcctaca tttcatactg tttctactcg
    42361 tattgacgaa ttgtatacga ctcatgcgtg gacccaaggc ggaaaagtaa aagaattacc
    42421 aggagatatc attttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa
    42481 caatgcaaaa gaaaccaatg atttttggaa aaaagtcaat caagaattag aagaagccta
    42541 tcaaaaggga actatcaaaa agaaaaaaga attctatctg atgggatcag gggatggaaa
    42601 gacgatgaat gatctgccaa tcgtatttga ttttctgaaa tcaggatatg cttatgcatg
    42661 gcgatacaaa gatttccatc aaggggggaa ttatagtttt gggacgcaag aacaagtcga
    42721 acaggcacaa gaattattga atcttccact aatggataat tggatcgata gcagtgatcc
    42781 gagaacacct gatttccagt tgacaaaaac agcaaaagtc gcgaatgtca tgataaaaat
    42841 ttaccaagtt ctggccattc ccgtgctgat tgcttcaata gcagggatat tacttatgat
    42901 actaggaacc gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtagt
    42961 catcggatta atactgacac aattggtttt tcttttcggt gtctcgtggt tttgttctta
    43021 tgcgccagaa aaaaaggatt attttttaag tgtctacact ggcgcaggcg tgccattaac
    43081 gcaattcatc gcagtaacaa gcatgctagt ttttagtaaa ttgaatctga aaaaagcttt
    43141 atcttccaaa taaaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata
    43201 aacggtg
//
LOCUS       DACECB010000018        41529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECB010000018 DACECB010000000
VERSION     DACECB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.018"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO04_001045"
     CDS             214..498
                     /locus_tag="IWO04_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0212671.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO04_001046"
     CDS             complement(779..1198)
                     /locus_tag="IWO04_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0212672.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IWO04_001047"
     CDS             complement(1200..1784)
                     /locus_tag="IWO04_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0212673.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO04_001048"
     CDS             complement(1831..3306)
                     /locus_tag="IWO04_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0212674.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO04_001049"
     CDS             3487..3798
                     /locus_tag="IWO04_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212675.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3914..6250)
                     /locus_tag="IWO04_001050"
     CDS             complement(3914..6250)
                     /locus_tag="IWO04_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212676.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6392..7060
                     /locus_tag="IWO04_001051"
     CDS             6392..7060
                     /locus_tag="IWO04_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212677.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7063..8106
                     /locus_tag="IWO04_001052"
     CDS             7063..8106
                     /locus_tag="IWO04_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0212678.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8343..9224)
                     /locus_tag="IWO04_001053"
     CDS             complement(8343..9224)
                     /locus_tag="IWO04_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0212679.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9383..10111
                     /locus_tag="IWO04_001054"
     CDS             9383..10111
                     /locus_tag="IWO04_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0212680.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10267..10584
                     /locus_tag="IWO04_001055"
     CDS             10267..10584
                     /locus_tag="IWO04_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0212681.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10577..11185
                     /locus_tag="IWO04_001056"
     CDS             10577..11185
                     /locus_tag="IWO04_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0212682.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11201..12031
                     /locus_tag="IWO04_001057"
     CDS             11201..12031
                     /locus_tag="IWO04_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0212683.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12169..12657
                     /locus_tag="IWO04_001058"
     CDS             12169..12657
                     /locus_tag="IWO04_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0212684.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12708..13580
                     /locus_tag="IWO04_001059"
     CDS             12708..13580
                     /locus_tag="IWO04_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0212685.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13756..14118
                     /locus_tag="IWO04_001060"
     CDS             13756..14118
                     /locus_tag="IWO04_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0212686.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14127..14879
                     /locus_tag="IWO04_001061"
     CDS             14127..14879
                     /locus_tag="IWO04_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212687.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14893..16695
                     /locus_tag="IWO04_001062"
     CDS             14893..16695
                     /locus_tag="IWO04_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212688.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16972..18012
                     /locus_tag="IWO04_001063"
     CDS             16972..18012
                     /locus_tag="IWO04_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0212689.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18064..20208)
                     /locus_tag="IWO04_001064"
     CDS             complement(18064..20208)
                     /locus_tag="IWO04_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0212690.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20565..21938)
                     /locus_tag="IWO04_001065"
     CDS             complement(20565..21938)
                     /locus_tag="IWO04_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0212691.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21938..22333)
                     /gene="rbsD"
                     /locus_tag="IWO04_001066"
     CDS             complement(21938..22333)
                     /gene="rbsD"
                     /locus_tag="IWO04_001066"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0212692.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22361..23278)
                     /gene="rbsK"
                     /locus_tag="IWO04_001067"
     CDS             complement(22361..23278)
                     /gene="rbsK"
                     /locus_tag="IWO04_001067"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0212693.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23386..24399)
                     /locus_tag="IWO04_001068"
     CDS             complement(23386..24399)
                     /locus_tag="IWO04_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0212694.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24480..25079)
                     /locus_tag="IWO04_001069"
     CDS             complement(24480..25079)
                     /locus_tag="IWO04_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0212695.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25265..25966
                     /locus_tag="IWO04_001070"
     CDS             25265..25966
                     /locus_tag="IWO04_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212696.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25976..28687
                     /locus_tag="IWO04_001071"
     CDS             25976..28687
                     /locus_tag="IWO04_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0212697.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29138..30766
                     /gene="treC"
                     /locus_tag="IWO04_001072"
     CDS             29138..30766
                     /gene="treC"
                     /locus_tag="IWO04_001072"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0212698.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30826..32007)
                     /locus_tag="IWO04_001073"
     CDS             complement(30826..32007)
                     /locus_tag="IWO04_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0212699.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32259..33782
                     /locus_tag="IWO04_001074"
     CDS             32259..33782
                     /locus_tag="IWO04_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0212700.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33851..34579)
                     /locus_tag="IWO04_001075"
     CDS             complement(33851..34579)
                     /locus_tag="IWO04_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212701.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34592..35872)
                     /locus_tag="IWO04_001076"
     CDS             complement(34592..35872)
                     /locus_tag="IWO04_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0212702.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36013..36615)
                     /locus_tag="IWO04_001077"
     CDS             complement(36013..36615)
                     /locus_tag="IWO04_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0212703.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36860..37066
                     /locus_tag="IWO04_001078"
     CDS             36860..37066
                     /locus_tag="IWO04_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212704.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37378..38097
                     /locus_tag="IWO04_001079"
     CDS             37378..38097
                     /locus_tag="IWO04_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0212705.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38178..38861)
                     /locus_tag="IWO04_001080"
     CDS             complement(38178..38861)
                     /locus_tag="IWO04_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0212706.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39079..41265
                     /locus_tag="IWO04_001081"
     CDS             39079..41265
                     /locus_tag="IWO04_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0212707.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13482 a         7354 c         8324 g        12369 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
     3841 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
     3901 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
     3961 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
     4021 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
     4081 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
     4141 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
     4201 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
     4261 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
     4321 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
     4381 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
     4441 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
     4501 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
     4561 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
     4621 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
     4681 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
     4741 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
     4801 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
     4861 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
     4921 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
     4981 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
     5041 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
     5101 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
     5161 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
     5221 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
     5281 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
     5341 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
     5401 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
     5461 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
     5521 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
     5581 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
     5641 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
     5701 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
     5761 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
     5821 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
     5881 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
     5941 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
     6001 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
     6061 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
     6121 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
     6181 caccatccaa tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta
     6241 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
     6301 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
     6361 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
     6421 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
     6481 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
     6541 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
     6601 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
     6661 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
     6721 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
     6781 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
     6841 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
     6901 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
     6961 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
     7021 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
     7081 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
     7141 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
     7201 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
     7261 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
     7321 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
     7381 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
     7441 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
     7501 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
     7561 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
     7621 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
     7681 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
     7741 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
     7801 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
     7861 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
     7921 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
     7981 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
     8041 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
     8101 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
     8161 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
     8221 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
     8281 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
     8341 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
     8401 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
     8461 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
     8521 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
     8581 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
     8641 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
     8701 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
     8761 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
     8821 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
     8881 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
     8941 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
     9001 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
     9061 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
     9121 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
     9181 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
     9241 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
     9301 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
     9361 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
     9421 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
     9481 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
     9541 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
     9601 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
     9661 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
     9721 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
     9781 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
     9841 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
     9901 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
     9961 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    10021 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    10081 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    10141 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    10201 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    10261 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    10321 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    10381 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    10441 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    10501 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    10561 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    10621 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    10681 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    10741 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    10801 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    10861 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    10921 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    10981 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    11041 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    11101 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    11161 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    11221 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    11281 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    11341 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    11401 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    11461 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    11521 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    11581 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    11641 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    11701 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    11761 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    11821 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    11881 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    11941 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    12001 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    12061 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    12121 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    12181 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    12241 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    12301 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    12361 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    12421 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    12481 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    12541 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    12601 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    12661 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    12721 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    12781 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    12841 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    12901 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    12961 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    13021 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    13081 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    13141 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    13201 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    13261 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    13321 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    13381 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    13441 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    13501 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    13561 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    13621 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    13681 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    13741 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    13801 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    13861 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    13921 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    13981 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    14041 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    14101 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    14161 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    14221 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    14281 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    14341 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    14401 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    14461 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    14521 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    14581 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    14641 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    14701 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    14761 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    14821 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    14881 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    14941 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    15001 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    15061 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    15121 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    15181 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    15241 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    15301 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    15361 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    15421 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    15481 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    15541 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    15601 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    15661 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    15721 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    15781 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    15841 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    15901 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    15961 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    16021 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    16081 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    16141 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    16201 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    16261 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    16321 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    16381 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    16441 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    16501 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    16561 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    16621 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    16681 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    16741 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    16801 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    16861 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    16921 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    16981 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    17041 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    17101 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    17161 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    17221 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    17281 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    17341 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    17401 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    17461 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    17521 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    17581 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    17641 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    17701 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    17761 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    17821 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    17881 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    17941 ggagatgtca ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat
    18001 ataaagaaat agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt
    18061 attctatttc tttaaaggca gatcaagcca gaatgatttg tctaaactta acaaggttag
    18121 tacaggaaca gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt
    18181 aaaattgttc cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac
    18241 gcgttttatc gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat
    18301 tggcacatcg aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc
    18361 tgcttctttt ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa
    18421 tttttgtgaa tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt
    18481 gtagtctttt gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc
    18541 atttttgatc aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa
    18601 ttgtgcgcgg atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac
    18661 gctgcttcct ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa
    18721 taagctgttt ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat
    18781 tccttctttt tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt
    18841 tgtttggttt tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc
    18901 tgtcagcgaa cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg
    18961 cgtgaaatct gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa
    19021 ttgtccgttc ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa
    19081 gacgaggtcg ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc
    19141 gcctgtccag ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc
    19201 agaacagtca aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt
    19261 gaatgcttct cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc
    19321 aattggtgat tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg
    19381 atttgctgta tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt
    19441 tggatggttt ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg
    19501 attcgaagaa ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt
    19561 attattgttg ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg
    19621 gacttcttca ctaggatcag ttggtttctc tgttgaagta tccggtccag ctttaaattc
    19681 tgcagtaata gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc
    19741 tttggcacca ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta
    19801 agttaccttc gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg
    19861 ttgctgttca ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg
    19921 agcaattgta agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc
    19981 aatttcagtt ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt
    20041 aaaagaatcc gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt
    20101 ttgatcagct atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc
    20161 aatcagccaa gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc
    20221 cttttccttt cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga
    20281 taatcgtgtc gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa
    20341 tttgtaaata agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga
    20401 ccacctttgt ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata
    20461 ccaaagtttg gaaagtatca caaaaattta taaaaataat gatcctaaaa agaaaaagac
    20521 tgaaatatgt gataaacatc tcatacttca gtctttttca ttaactattc ataagacatt
    20581 aggtcttcat caaacccgct aacgagagta gtgatcattg ctacgactag actaacagcc
    20641 agtcctgcta agtaccagaa aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta
    20701 ccaaatacaa aagcgatcgc tttcacttgg gcaaatccca ttacagctcc accagcagct
    20761 gcaccaatgc aagcagcaac aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt
    20821 ggttcggtca ctcccgcaag taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt
    20881 tttgaacgtt tacttcgcag aaatacacct aaagctgcac cagccattcc aaaattggct
    20941 gctcctgtca acggaacgat tcggtcgaac ccataagcag agatattact gatcatgaca
    21001 gggtttacag cccattggat accaaatatt accaaaatat tccaaccacc agcgatgatt
    21061 gcgccactga tcggtccgtt aatatccatc atccaagtaa ttgcggcagc taaaccttcg
    21121 ccaaggtaaa cacccgcagg cccgaatacc attaacgtca aaggaaccgt gataactaat
    21181 gaaagtaaag gtacaaatac taattgcata ttttcgcgaa tatatttttt gaggaatttt
    21241 tcaacaaaag aataaaagaa aatacctaaa atcgccggca tgacagtaga cgtatactgc
    21301 atcagtacta caggaatccc cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa
    21361 gaggtcagat tcggttcaat cagtgctgca gcaatgacag ccgcaatata tccattcact
    21421 ttaaacgttt tggcagcaga aaaagcaaga aagattggca gaaaataaaa gacggcattc
    21481 gacgcagcag acaaaatatg ataagttccg cttgtttcac taagccagcc aatcgtagtc
    21541 gctaaaacaa ccagaccttt taatatacca gaaccggcaa acagcggaat gatcggcgta
    21601 aagatttccg atatcaaacc aaatagtttt tcgatcggat ttagcttttc tttcggagct
    21661 gaatgatcga tttgacgcgt caattccttg taagcaggag ccaatccctg atccatgatt
    21721 atttctgcat atctatcgag taaaatgaaa tacttaatgg aagggatttg ctgttttaat
    21781 ttttctgcat tggctactgg ctgttttttc aatttgatac gaagccgagt cgcacatgga
    21841 aggacagaat caatattgtc ttttcctccc aactgttcaa tcagcatatg aatctgctcg
    21901 gacagtacca tctgttcagg attcattgtc gtttccatta gaagaccact ccactcacta
    21961 atataatatt ggcgtaaggt gtcatttcgc ctgtacgaat aaatgcatga cagttattca
    22021 actctttttt catttcttca tgtgggataa agctaatcgg tgtatctggt aacaataatt
    22081 tgattttttc caatatttcc gggttcattg tctttatttc ttccgccaat tcaatcgatt
    22141 gtacctcaag ttcttctaaa acgttttcta aaacagataa aaaacttgga agaccgtttg
    22201 agacagccaa atctattttt tcagtaccaa aggggacagg catgcctgca tccccgatac
    22261 ttaatttatc aaagtgcccc atttgtgcga ttactcgtga aatatctgaa ttgattactt
    22321 ttcctttttt catgtttgtt tcttcctctc attgttaatt ttagactgct gattgttcta
    22381 aagcttcgcg gtaagggata gaaggttgcg caccataaga ctgcacagca acagaagagg
    22441 ctagatttcc ataaaggatc gcttctttca agttactaag gtctggctga agtgtgctgc
    22501 ttaatgcgcc aataaatgta tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag
    22561 cagggacgat accgtgttct ttttctgtat gatagaaagc gccggcactg cctagtgtaa
    22621 tgattactgt tccaatacct aattcatgga gtttttcggc tgctgcgacc aaactgttat
    22681 gatctgtcac tctgattcct gtaatgattt ctgtttctgt ttcatttggt ataatgatat
    22741 ctgtcttttc aagtagttca gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg
    22801 ttgtttttcc tgcgtctttt gcaatagaaa aagctaggat cgtcgcatct aagctacttt
    22861 caaactgagc aataataaag tcactgtttg taatcagttg cttgtttttt aggacttctt
    22921 gttcatggaa agcattattt gcccctgcat ggatcaagat actattttct cctgctgcgt
    22981 ctaccataat cattgcttgt ccggttgtgg cgccttggat ttcagctatc ccagatgtat
    23041 cgatgttttc ttttgtcagt agatctaata actgttgtcc ttcgctgtca gccccaacgc
    23101 ctccaataaa agatgtccgt gcgcctgaac gtttggctgc cactgcttgg ttggcacctt
    23161 tacctccgcc agcgtgaaaa atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt
    23221 tggttaaacg aagggtagtg tccatattaa tacttccaat tactgtaacc gtgttcattt
    23281 tacaaacctc caactatctc atttattttg atgggaaaat tattttttaa aacgttttag
    23341 taaaacgttt taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc
    23401 agttgaaaat cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg
    23461 gaattggatc cgatctaaca ataacttggc agatgtttgt cccaattcga agattggttg
    23521 tgcgatcgtt gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat
    23581 aatgctgtag tcttcaggga tgctttttcc agcttcctct aatccgcgat ataatccaaa
    23641 agctaattca tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt
    23701 gatctggtag ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg
    23761 gatttggtaa acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg
    23821 tggattttca ggatagacaa gggcgatcgt ttgatgtcct agactaagca agtgcatgcc
    23881 cgctaggtaa ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt
    23941 tttctgatcc aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc
    24001 tgtagagaca gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc
    24061 tagatattcg atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt
    24121 ctgcttatac aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat
    24181 atcaggaacg agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata
    24241 gtcaggctga tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact
    24301 gaatttctcc gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag
    24361 tttagcgact tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc
    24421 ctgtggttat tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact
    24481 tatttgcttt gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc
    24541 ggaaatatct tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct
    24601 tcagcaagca cgatatggta cccgtgttga aaggcatcta atgcagtagc atatacaccg
    24661 tttgttgtcg ctacaccgct taaaatgata gtttcgattc cacgccggcg aagttgaaga
    24721 tcaagagaag taccaaaaaa agcacttgga ttgtattttg tgatagtcaa catattatct
    24781 gtatctttca aagaatcctt caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc
    24841 attccaccca tatcagtttg ttgactcaaa taagggaaag tggagatgtc tacgttcact
    24901 aaaactttca aggcagcggt acgtttcaat gttcttacta acaaatcatt gtttgtcaga
    24961 atagatgctg cagtgtgtgg agagagtgta ccgttattta cgatctcttt ttgcatgtcg
    25021 atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa atcattcatg
    25081 atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat cgtttatttg
    25141 tcaactaaat taaaggattc ctttctttag tctagagaaa ttcagtggta aaataaataa
    25201 gtgtgagaaa acgcattcga tgcatagata tagcgagaaa tatcgtgaaa atggaggaat
    25261 cagagtgaag tatgtcgaag taaaaaatga atacaaacga tacaaaatgg gagaaacgac
    25321 cattacagca aatgacgata tttctttttc aattgaaaaa ggcgaattag tgattatact
    25381 tggtccaagt ggagcaggaa agtcaacgat tcttaatatt ttaggtggga tggatacacc
    25441 tgatgaaggt cagatcatta ttgatgacac agatattgcg caattttcag ataaacagct
    25501 tactgcttac cgacgaacag atgtagggtt cgttttccaa ttctacaatc tcgttcctaa
    25561 tctaacagca aaagaaaatg tggaattggc aacggaagtt tctcctgatg cgcttgatcc
    25621 tgtagaggta ttgaggcaag tgggcctagc tcatcgattg aataactttc cgtcccaatt
    25681 atcaggaggc gaacagcaga gggtttcaat tgctcgtgca ctagctaaaa atcccaaact
    25741 tcttttatgt gatgaaccga caggcgcgct ggattttgaa acgggcaagc aagtattgaa
    25801 attattgcaa aacgctagca gacaacacgg caataccgtt ttgatcatta cacataattc
    25861 tgctttagcg cccatcgctg atagagtcat tcacattaat gatgctaaag taaggtctgt
    25921 tgaattaaat gatcatcctt catccattga tgagattgta tggtaggggg attttatgaa
    25981 aacaagaaca tatcttaaag caggttttcg cgaaattcgg caatctaaag gacgattcat
    26041 tgcaatcatc cttattgtca tgctaggaac acttttattt gtaggtgtca aaactgctgg
    26101 acctgtcatg caaaaaacga tggatcacta tgttggtaca gcaggacttt ctgatttgca
    26161 aatcgtttct actggtggct tgacacaaaa agatattgct caagcagaaa agatcccaga
    26221 tgcaaaagta gaaacaggaa aacagatcta ttattccaat actggcaaaa acgaagtgat
    26281 tcaaatattt tcttataaca aaaataacaa gcagaacaag ctacaattaa ctgatggaaa
    26341 actcccggaa aaaccaaacc agcttgtatt ggatgaaaaa gcggaagatg aagggtataa
    26401 aatcggtgat atttatcaga ttgattcaga tgaattgaaa gaaaaagagt ataccatcac
    26461 gggctttgtc cgttctccat tgttcatcaa caatttggag agaggatacg cgaacgtcgg
    26521 aaatggtagc gtcgattatt ttgtctattt acctgaatcc gtttttaaaa cagatattta
    26581 ttctgtctta tatcttgatt ttacaaacgt aaagaatttg aatacgtaca gcaaagctta
    26641 caaagataaa atggagcaaa atcaagaaaa ggcagaaaag tatttacaag atcgacccga
    26701 agaacgctta gaagagctga aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa
    26761 aatagcggat ggcaaagcac aaacaacgca agcaagaaca cggttagaag atgctaaaaa
    26821 acagttagcg caacagtcag caactatcca gcagcttcct gaagcgcaac gaacaatcgc
    26881 tgttcaaaca ttatctcaac aagaagctca acttgctgaa caagaaaggc aattagttga
    26941 gaaagaacgt gaactggcct ctgcagagga agaattgaat cgaaatgcag aaaaaattga
    27001 aaatttaact aaaccatcat acctatacaa tgaacgttca gaaaatgtcg gttttcaaga
    27061 atttggtgat ctgtctgatc gtatcgctgc gattgccaat gtttttcctg ttttcttttt
    27121 ctttattgcc gcattgatca cctttacgac aatgacacgt atggtagaag aaaaccgcag
    27181 ggaaatcggg acattaaagg cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc
    27241 aatctatgca agtctagcct cagggatagg tattgtttta ggaacgatat taggaacgaa
    27301 tcttttgcca aggatcattt ttgagctgtc taacgagcgg tatgatattg ggagtgcgct
    27361 tattttttat gactggccgc cgattttaca agcagctgcc gcattcttca ttgccgcatt
    27421 tggcgctgca atgatcgttt tattcaaaga tttaagagaa cggcctgctg cattgcttca
    27481 accgaaagca ccgaagccag ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc
    27541 acgccttagc tttaatcaaa aaatcagtta ccgaaatctc ttccgatata agtcaagaat
    27601 gcttatggcg attatcggaa ttgctggatg tgccgggctg atggttgcag gcgtgggatt
    27661 gaaagattct ctaagttctg tttcagccaa acaatttgga ccgatcattg actatcaagc
    27721 aatcgttaca ttggattcat ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca
    27781 gaagcaagaa aaagtaaatg agatcctagc tattcatact caaaccgtag aattgagaaa
    27841 aaaaggacat gcttcgcaaa gtctgacagt gatcgtacca agtaaaacag ctgatttgga
    27901 tcgttttatc catttaactg atcttgaaga gaacccacgc tccttgccta aaacaggaat
    27961 cgctattacg caaaaagtag cagaactatt tgacttatct acaggagatc atatttcgct
    28021 ttatgatgaa gatcaaaaag aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct
    28081 aggtagcttt atttatatgt cccctgaata ttatgcttca gttactgatc aatcgatgat
    28141 ggataatgca tttttgctgc gtacagataa gatgacaaca aaagaagaag aaatgctttc
    28201 cgagaaactg cttgccacag acgctgtgac aaatacgtct ttcatgtcca aacaaatcga
    28261 aacgcaagaa gaatcaatgg cgaacttaga ctcggttgtc ttaatctttg tcgtattatc
    28321 aggactgctg gcttttatcg tcttgtataa tctaactaat atcaatattt ccgagcgtgt
    28381 acgcgaatta tctacaataa aagtccttgg attttttgat aaagaagtca cgatgtacat
    28441 tgttcgagaa aatatcatct ttactctttt aggaatcatc ggaggatttg gtgtaggata
    28501 tatattgact gacttcatct tgcaacaagc gtctatggag aatgtcatct ttccattagt
    28561 gatcacatgg tgggcttatg tcttatcagc aggattaact atcgtattta cggttattgt
    28621 gatgattgtt acccatttca aattaaagca tatcgatatg attgatgcgt tgaaatcgaa
    28681 tgaataaaaa cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
    28741 caaaatagtt tttcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
    28801 caccatgaat cagaggttgt aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat
    28861 aaaattagct cctcatgttt tacatttgag aagactgatt gccgtagata aaagcttttg
    28921 aacaccattt atagaagaaa caagaatatt ttgctatttt aaacaagagg atagacaaat
    28981 acatgtctga tctcttgttt tttattgggc aacagacctc ttagaatgta taaagataaa
    29041 ttttacaaac gctttcggtt cagttataat aataaatgat aacttgtata tataagttgt
    29101 ggtaggtaat aaatcagttg aatggaggta aatatccatg acatttaaag acaaagtcat
    29161 ttaccaaatc tatcccaaat cttacaaaga cagtaatggt gatggaattg gagatttaaa
    29221 aggaatcaaa gaaaaactcc cgtatctgaa tgatctaggc atcgacatga tttggctaaa
    29281 ccctatttat ccaagccctc aaaaagacaa cggttatgat atctctgatt acacagccat
    29341 cgatcctatc tttggtacga tggaggaatt cgaacagcta atagccgaag caaagaaata
    29401 ccggatagaa atcatgctcg atatggtgct gaatcacgtt tcgacagaac atgaatggtt
    29461 caaaaaagca ctagcagggg accagcatta tcaggacttc ttcattttaa gagaagagcc
    29521 aacggattgg gtatcaaaat ttggtggaag tgcttggtcg ccatttggag atacggggaa
    29581 atattatttg cacttatatg atcggacaca agctgacttg aactggcgga accaagcagt
    29641 aagagaggaa ctattcaaag ttgttcgttt ttggatggaa aaaggggtaa aaggattccg
    29701 tttcgatgtt atcaatgtta tcggtaaaga cgaacaattg aaaaataata aagaaaatga
    29761 tggcaaacct gaatatacgg acaaaccaat cacccatacg tatctaaaag aattgaacca
    29821 agcaacgttt gggacggatt ccactatcat cacagtcggt gaaatgagct ctacgacgat
    29881 cgaaaattgt atcttgtata cagaacctag tagagaagaa ttatcaatgg tctttaattt
    29941 ccatcatttg aaagtcgatt atgaaaatgg gaataaatgg tctactcctc catttgattt
    30001 tgcagaatta aagcgactat ttcatacatg gggagaaaag atgagtgagg gtaacggctg
    30061 gaatgctcta ttttttaata atcatgatca gccacgtgcg ctcaatcgat ttgtagctat
    30121 cgatcagtat cgaaaacaag gagcagagat gctagcaacc atgatccatt tgaaccgtgg
    30181 gacgccctat atttatatgg gagaagaaat cggcatgatc gacccagact attcatcgat
    30241 ttccgactat attgacgtag aaagtttgaa tgcttataag ttactccttg aacaaggatt
    30301 cacagaaaaa gaagcgtttc gtcggatcaa agcaaaatct cgcgataact ctcgaacacc
    30361 catgcaatgg tcagacacag aacaagcagg atttactaca gggaaacctt ggttaaaagt
    30421 tggaggcaag ctagaagaga ttaatgtaga aaaagaacta tcttctgaag attctatttt
    30481 gtcctattac aagaaattga ttcggctgag aaaaacatat ccaatcgttg cacaaggaga
    30541 ttatcatgct tatgaagcaa atcatccgga agtttatggc tttcttcgcc aatttgaagg
    30601 acagcagctg cttgtattaa caaatttcta tgataaagat acaaggatcg ccatcccaga
    30661 aggattcaca gaagccaata ttcttatcga taattattcc gatactgttc ttcatcaaag
    30721 tctggagcta aaaccatacc aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa
    30781 acgtcgttcc tacgagctat tctcgtaaga tcgacgtttt gtgttttatt taaaataatc
    30841 ttcttcagaa aatgaagagt tttcatactc tcctttatcg ccataaaatt tattgtagcg
    30901 ttgtttgaat ttacggaaaa taaaagtaac gagtaatttg actaacgtat agataggaat
    30961 accaaaaata acgcccacaa ttcccattag atcacccatg acaagtaaaa cgattaagat
    31021 cgtaatcgga tggatctgta aacgatcacc cattactcga ggaatcacca agtcaccgtg
    31081 gagaagttgt acggcaaacc agacaatgac aaatttgaca gccataaaag tagagtcttg
    31141 gaatgcaatg atcaaacctg gaaagaaggc gataaacggc ccaatatatg gaatgataca
    31201 taacaaccca gcagaaatag ctaaaacact agcaaattct aatccgatca gtaagaaaca
    31261 aacccagtag actgctccaa ggataaatga agcaatgatt tgccccttta gaaatgaacc
    31321 caattgttga ttagcaatct ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac
    31381 aatcgctaaa actgactgat aaaatttttg aggatctctc aaaaggaaaa aagcgacaat
    31441 tggaccagta aataaagtaa taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg
    31501 ttgtgcgcca ctttcccaat aatttccaat aaatccagct atatcagacg tcacgtcgtc
    31561 aatagaagcg aaaaattgat taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg
    31621 gagactctct tgaaaatctt gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg
    31681 ttcttcaaga aaaggaataa gccatatgct tccaagcccc aataaaaata aaataatcac
    31741 ataaacgata atgactgaca agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg
    31801 ttccaaaaga taatacaaga tcagtgcaaa caataatggg ggcaagatag tagcgataat
    31861 gattccgaca ggcttgaata taaacgagat ctgatggaaa aaataaatga tcagcgcaag
    31921 taagataact attcctaaaa catagtaaga ggtttttcct cctaaaaagc ggatcaaacg
    31981 attcgttgta tctgtttctt ttttcactct ttttcctcct ttatctataa cttcattata
    32041 gacaaaagcg ggtataaaag gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat
    32101 tcatataaac tttctcatca attaaaataa gaaaacgctt gacagttctt ttaaccatat
    32161 gataaattaa atcctgttgc taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa
    32221 cattcaaatg aaagaagatg agaagaggag tgtcataaat gaaacgattg ataggtatat
    32281 tggccatgct ggttttagca ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg
    32341 acagtaaaga aaaattgagc gtgatgacga ccttttatcc catgtatgat tttacaaaag
    32401 caatcgtcgg ggatgaagga gaagtagagt tattgattcc agcagggact gattcccacg
    32461 attatgaacc ttctgctaaa gacatggcaa agatccaaga tacagatatc tttgtttata
    32521 atgatgaaaa catggaaaca tgggtccccg caatccaaaa aacattacaa gaaggcaatg
    32581 tccatacgat aaaagcgaca gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg
    32641 atcatgacca tgaacacggg gaagaaggcc atacacatga gctagaccca catgtatggc
    32701 tagctccaag tttagcaatc aagcaggtag caaacatacg tgatcagctg attgaagctt
    32761 acccagaaaa acaggaagta tggacaaaaa atgcagcagc ctataccgag aaacttcaag
    32821 cactccatca gctttatcaa gaaaccttta agcaagcaaa acaaagatct tttgtcacgc
    32881 aacatgctgc attcaactat ctagcgttag aatatggatt gaatcaagta tcgattgctg
    32941 gtctttcttc tagtgaagaa ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta
    33001 aagaacatgg aatcaactat atttattttg aagaaaacgc caaagatagt attgccagaa
    33061 cgttagcaaa cgaagcaggt gtatctttag aagtattgaa tcctttggaa ggattgacga
    33121 atgaacaaat agaaaatgga gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt
    33181 tgaaaaaaac gacagagaca gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa
    33241 agagcgtcta caacggttat tttgaagacg aggatgtaca agatcgctct ctttcagatt
    33301 gggcaggaac ttggcaaaca gtagatacgt atgtagaaga tgggactttt gatcctgttt
    33361 ttgaatataa agaaaaattg aatcaggata agacagcaca agaatataaa gaatattata
    33421 cgaagggcta tcaaacagat attgcttcaa tcaagatcag tgagaaagag atggcattta
    33481 cgtttaagaa tggaacaacg aaacgttcaa attatcgtta tgtagggaaa gaaatcttga
    33541 cctacgaggc aggaaaccgt ggcgtaaggt atctattcga agcagaagat aaggaaagtg
    33601 gcgcttttag atatattcaa ttcagtgatc acacgattgc accggctgac tccgaacatt
    33661 ttcatttata tgcaggaaat gaaagtcaag aagtattgct gaaagaaatg gagaattggc
    33721 caacatttta tccagataca atggatggac gtgaaattgc acaggaaatg cttttgcact
    33781 gataggcaaa aaagaaccga agggtttgga tcggctggtc atttagtcgg tccaaactat
    33841 tcggttcttt ttatgaaatc aatttcttta atccacggat agaggctggg atggtctcgc
    33901 cgtttgccaa cgtgacttct ctggtttttt tattaatcga ccgcacattt tgtgtattga
    33961 taacatagga tttatgacaa cggataaaat tccgatgcat tttctcgatt tctttgatct
    34021 tctcatagaa ttgtaattgg gcatgagtca aatggacttc cagtttatgt ggcgctccta
    34081 gcgaagtaga aaaatattgg atgtctttga tcggtaaaaa tttgatctca tcattaaact
    34141 ctattttgaa gatatctttt tggatttggg acatttgaaa atcttcgaaa attttgctca
    34201 ggatatgctc gacattttct tttaaatcat aaagatcttc tttgacgata taatcagtcg
    34261 gttcgatgtt gctttttaag attttcatcg acatttccgt atgggaagtc acatagatta
    34321 tttttcctaa aggatctaat tcacggatca atttaccgat cgctacacca tccatttcac
    34381 tgtcttctag ttcgatatct aaaaagtaaa taccaaaaga agaagttctg atcttggctg
    34441 cattgagaag ttcttccgga tcagaggtag aaatatggac ataactgtct aagttttcaa
    34501 tcatgattct atttttgatc accgttgata aaacttctaa ttgttttgga tcgtcttcac
    34561 aaatataaat gggaatcata tcattactcc tttattttat ttgtatataa atcgtttgag
    34621 aaaaacgctg gttttcaatt gatgtttcaa ggaaaagata aggattggtc tttgtcaatt
    34681 catccaagat aaacaagcca aggccttcgt tttttggatt tgtttttgtt gagaaccctt
    34741 tttgtttcaa ctggtttaac ggtaatgtat ttgttgttgt attgatcaca cgaatcatca
    34801 cggaatgatt cttcttgagt atggtcaatt cgattttttt cttctttgca gcactagctc
    34861 cttcaatcgc attgtccaag ataataccaa gcatacgtac aaggtttacc gtatgtttcg
    34921 tatccagttc gattggctca ggaaaatgaa gctggacgat caaccctttt tcctgtgcca
    34981 tcatgagttt taatgacagt aaactacgga tctcaggaac tttcatatta tccaacatac
    35041 ttagattggc cggaaaaagg ttcatcatct ggcgtgtagg taatatcgtt tgatcataat
    35101 aagaccgtaa tccttctaag tcgccttctg aaatatagct gtgaagagaa aaaagcagat
    35161 tttggtaatc atgtttgaat tctctcaagt cttgatagtt tttttctaga tctttgatat
    35221 aatcctgttg catcctgact tgttgctcta ataactgatc agctaatttc ttattagaga
    35281 ttttccaata ataaaccaag atgatcaggc caacacacag atatagaatg aaaaaactcg
    35341 tattcaaagc taagacatca ggtgtttcac cagaaaaacg ggtataaaaa ataatgatat
    35401 agtatgtaaa taatatcaat gtcccgatcg taccgatcat acggcggtcc acagtgtgaa
    35461 accaagtctc taacgatttt tttaaacaaa aagctaataa agcagataaa aaagcggata
    35521 agacaatgtg aaaatactgc aatgaatctc ctggttcaat aaccgaagat ttcaaaacaa
    35581 aaaaatcgcc gatgcttgtt aagtgatctc ctaaaagcga cgtcaataac ccaaaaggaa
    35641 gcgcgtaaac caattgttga cgcgaagtcg aaaagaagaa aaaagtaagc atcagcattc
    35701 ctatagcaat tagagcagta aaaatcccta aaatcgaata gacccatcca atcaaaatac
    35761 aagaaatcag aatgactccc gcaaacagat atttgtatcg gatgtgatgg tcaatcaata
    35821 agatacaaat tgtgtaaatc aaatattgca acgctatgga ccataaaaac ataaagtaca
    35881 gctcccattg atttttacca aattatagca caattggcgt attatttttt taatttttca
    35941 gggattttga attcagaact cataccaaag cagcagccag atacaaaaaa gtcagataag
    36001 ttagttaatt gtttaatgat tgtttttttc atgaaagatt cctccaaaat tcatcaagta
    36061 gtttgtaata ggaagcaacg tagctatttc taaaacaatc ccaagtagca aataggtaga
    36121 aaagtaagaa gctgagaaaa ataacgtaat gagagaaaaa attagaaaca tgctgatcgc
    36181 cttctttaat aatgggggac gtttctcggg ttcaatggga ttgattgctg tacctcttga
    36241 tccatagcga agcagtaaca aaaagcagct gattaacaaa aagaccatca ttgggaagga
    36301 gatagtcaat tgcagatgtt tcatcaccaa gggaacgccc cataaataaa gtagtgtcca
    36361 cagcagacaa acgaattcag agtcggaatg caatccataa gcatatctgc ggatgcagat
    36421 ataagaaaga aaggtgattc ctgtttccgc ccatgagcct gtcaccagag aaaagactag
    36481 aacgaccgct agttttccaa gattattcaa taaaacttct aagacaaatt ttacttggac
    36541 atacaccata tcttcttccg atttgtctcc gctgaataag gcttctaata atttgttcac
    36601 aactttatct ttcatctcat acgtacctcc accacaaatt ttatgctaaa aaaaataaaa
    36661 aacgtctttt gtttcataaa aagtggtttt gacatcaaaa ccgtcatgat ctcctaaaaa
    36721 aatcttcgtt ttccaagaac cttaccaaaa aaagtgacag ttttgatacg aaaagtactt
    36781 tttatgacaa gtttgtgctt tttcgagtga tttatgttaa aaaattactg accttataaa
    36841 aggtgatgga gggattctta tggaaaatga acaattgttt gaagtcggcg aaacatatgc
    36901 atttaaatct gatttatttg aatcaccaat aaaaggaaaa atagaaaaaa tctatgaaaa
    36961 tagtgtgatg atacgtgtca tgaaatgcgc agtagaagac aatgataccg ctcataatct
    37021 gcactggcag ttagttgtac gcaaatcaag tacaatagaa gaataaaaac agataaaatg
    37081 ttgagagcat aaagaggttg tgagtctccc gtttagctct gagttacaag gaataatgtt
    37141 aaaaaacagc ttttcatgtt ttatgaatat tgccttgtga ccgaggtgtt ggcaggagaa
    37201 tactgtttat ttgaaaaaag ggctgcgaca agacttttgt cacagccttt attttcatga
    37261 ataggagtta cttttggcta cttttagaga agtagctttt aaggagcaga taaagtcaag
    37321 gcttttattt ctataaatga agtagtaaaa tgaagaacag attttattac aggaaggatg
    37381 ccgataattg tgatcattgt tgcaggaacg atcggtgcag ggaaaagcac actaactgaa
    37441 atgcttgccc aagatttaga aacaaaacct ttttatgaga acgtggaaga taatgaagta
    37501 ttgccgctat tttattctaa tccagaaaag tatacttttt tattacagat tttctttttg
    37561 aacaaacgct ttttggcaat caaagatgct ttcagccatg atgacaacgt actagaccgc
    37621 tccatctacg aagatagtat gctgtttcat ttgaatgcag atttagggcg tgtttctgaa
    37681 gtagaagtaa agcagtatga aggtctttta gaaacgatgc tgaaggagtt agaagaaatt
    37741 tcgccacaaa agaaaccaga tctactggtt tatattcgtg tatcctttga aactatgtta
    37801 gcgagaatca aaaaaagagg ccgggaatat gaacagctag aacaagatcc tgaactctat
    37861 tcctactata aagaactcaa tcgacgatac gaagagtggt atgaacagtt tgatatatgt
    37921 cccaaaatag tgatcgatgg ggacaaatat gatttcgtag ccgatccggc atgtggagaa
    37981 caaatcgtac aagaaatcaa gatgcgggca aagaaaatga cagaagaggc agatgcccag
    38041 tcgatccgct acactacaaa taacccgaaa gcaaagggaa tctttacaac taaataatat
    38101 aaaaactaag caaattgctc actcgcagat atttctctgt gagcagcatc ttgcttagtt
    38161 tttttgattc attgagatca aatagtgaac cgattcgtat gataaaattg ccataaatga
    38221 aatgtctgag tgagggccat gatcaaaaat aaaatggaaa cgatcaaaat cgaatgggcg
    38281 ccaaacagat gatgacagaa aacaaggaca atagctcctg cgataaaggc aagaacgaca
    38341 cttagatagt ggaaaaatcg atcaaaatct gctttttctt tagaaataca ataatcatat
    38401 aagttcgttg ctaccccttt taaatttcca gttgtaaata aattagtgta ggcacgcccg
    38461 ttgattttat caaaagctac ccactggatc gatgcagtaa aagcaatcaa aatcgttact
    38521 aatgcgggat gctcatggac gggtgtcaaa ctaacaaaga aaaaaatcac tgcttcataa
    38581 tatagaacaa acaagcgcca gaaatgtttt tctttttttc taaaataatc aattagatac
    38641 tttgttagaa gaatccctaa gaaaaagaaa agggtagaca atactttttt accaacactt
    38701 agccattctc cgtcaaatgc ttggatgatc gatagaatga tattgccagt ttgagcagag
    38761 gcaaaagcac catattggat ataagtataa ctgtccatgg ctcctccgat aaatgtcagc
    38821 aaaagtccta ttgtacgccc ctcgtgaaag gaagtctgca tttttttcat caccttttat
    38881 gaatattttt gtcttcctta tcttagcata aacgattgaa aaaaaggaaa aattttcaga
    38941 aaactaaaat catatcgctt cccattttca tttttagaaa ggtctgttag tataaagaaa
    39001 aaatagacaa cgaaacttta aataaccaac ttgaaataaa caaattcaaa gaagaaagat
    39061 gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc gattacaaaa agaattgaaa
    39121 cgctgggaga acaagtataa aaagcttcaa caagtctttc ggctacaaga agaaatcatt
    39181 gctacctatg aagcggctta tggcgaagca cccggactta ttgaggaaga agtacaagta
    39241 aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg agaaacgttc ctataatcgc
    39301 ttcaatcaag aacaaaaagc agctatcaca tatgatatga agaaacatct tcgaatcata
    39361 gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa aagctgttta tctgatgacg
    39421 caaaaacaag tagacgaaga acgcatacta atgatcactt ttacaaggaa cgctgcaaat
    39481 gaactaaaaa aacgagtaga taatttcagc cagagaaaaa ccgctgtcca tatcggaaca
    39541 ttccatagta tcttttttag gatatacaat gaaatttgtc gaaaatttcc tgaagtagca
    39601 atgcaaggaa tacaaggaga cttttcaaag gatactgcac aaaaagtgaa tgctgttttg
    39661 caacagctga tacgtaaata taatctttat atatttgatc aatatggaga aaaaacgatt
    39721 gcttctcggt tagactattg gcagaatatg aacctttcag tagaagaaat ggttcagtta
    39781 gttaaagaaa aatatgattc cattgataag aatgcacgac agccgatatc agaacgactg
    39841 tatggattgc tcacggaact ccaagaacaa aaaagaagcc agcaattgct agagttcaat
    39901 gatgtactcc aaaatctgaa aagcgctttg gagaatgagg agatccagca ttatatcggc
    39961 caaaaatatg attatctatt cattgatgaa tttcaagata ctaatccact tcaatgggaa
    40021 atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga ttgttgttgg agatgatgat
    40081 cagagtatct atggattcag aggatcagag ccagcataca tcaagaactt tgaaaaagaa
    40141 tatcctacca aaactctttt cttattaact aattatcgtt ctcgggcagc tattgtccaa
    40201 ggagcaaata gattgatcag ctataataaa aatgatcgga taccaaaggc gatgattcca
    40261 gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt ctgatactaa agcagaaagt
    40321 cagtggctaa tcaaacaaat tcagacattt attcaaaaaa atgggaaata taaggaatca
    40381 atcatattat accgatcagc ttcacaaacg cagcagcttg tccaatctct gctgaaaacg
    40441 aatattcctt ttgtactgga agcagatagt ccatatgaag gtatctttgg tatcaagtct
    40501 ttccaacatt tctatcaaaa aattgttcat tggcaaacag cactgaatct gcaaaagaaa
    40561 aatcaggcct accagcaatt gctccggcag ctgatggcag actgctacct gaaaaaaagt
    40621 gaatgcgatc aatacttttc tcctaaatat caaaatatag ccgttgcaga ttatattttg
    40681 tcgataagcc ccaatttaaa atcgaaatct gcagaaatcc gagagtttga ggaagcgttg
    40741 cgtaaaacag accagcatca agaaatctat tcgcttgttc aaagctatct tcatctgcca
    40801 cgtatagcta aggaaatcga ccagagtgat caagaatgga tacgagaaga agtaaagcag
    40861 catgctactt tcagaagact ccaagcctta tcgcaagaaa cccaagcgac agctaaagaa
    40921 ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg atgcactatg tatccagagc
    40981 attcacaaaa gtaaaggtct tagttaccga aatgtctttt tgattggttg taacgaagga
    41041 agtttgcctt ataacggagc gacagaaaag aaagtaatcg actttaatga aataaaagca
    41101 gaaccagtaa cgacgataga agaagaacga agactatttt atgttgcgat gactagagca
    41161 agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt caagaaaatt gaaagtatcg
    41221 agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa agtgatagaa gagagggtgt
    41281 gacagaagtg gtcagtttca agaaagaaga aggaatgtac gaaaattgct tctcaaattt
    41341 ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg aacacagttt attcggaaaa
    41401 gggaggtgtg acaagaattt tgtcacacct cccttattta ttttgaaaat gagaatacta
    41461 ttatcaaaat agagaatata tagaaaaatt ctcattattc aaacgtaaat aatcagttat
    41521 tttgatttt
//
LOCUS       DACECB010000019        38322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECB010000019 DACECB010000000
VERSION     DACECB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.019"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1778)
                     /locus_tag="IWO04_001082"
     CDS             complement(<1..1778)
                     /locus_tag="IWO04_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212708.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPA"
     gene            complement(1765..1983)
                     /locus_tag="IWO04_001083"
     CDS             complement(1765..1983)
                     /locus_tag="IWO04_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212709.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(2059..3111)
                     /locus_tag="IWO04_001084"
     CDS             complement(2059..3111)
                     /locus_tag="IWO04_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0212710.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(3264..3590)
                     /locus_tag="IWO04_001085"
     CDS             complement(3264..3590)
                     /locus_tag="IWO04_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212711.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3602..3802)
                     /locus_tag="IWO04_001086"
     CDS             complement(3602..3802)
                     /locus_tag="IWO04_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212712.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            3877..4527
                     /locus_tag="IWO04_001087"
     CDS             3877..4527
                     /locus_tag="IWO04_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0212713.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4582..5748
                     /locus_tag="IWO04_001088"
     CDS             4582..5748
                     /locus_tag="IWO04_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0212714.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            6165..9392
                     /gene="fss3"
                     /locus_tag="IWO04_001089"
     CDS             6165..9392
                     /gene="fss3"
                     /locus_tag="IWO04_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0212715.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            9504..9818
                     /locus_tag="IWO04_001090"
     CDS             9504..9818
                     /locus_tag="IWO04_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0212716.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9831..10205
                     /locus_tag="IWO04_001091"
     CDS             9831..10205
                     /locus_tag="IWO04_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0212717.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            10215..10559
                     /locus_tag="IWO04_001092"
     CDS             10215..10559
                     /locus_tag="IWO04_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0212718.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDIPDWLGEEESFFMVVEVNESLCRQAESVLEEIGVEMPDAIEGFLKQL
                     VETKQLPVVVND"
     gene            10628..11644
                     /locus_tag="IWO04_001093"
     CDS             10628..11644
                     /locus_tag="IWO04_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0212719.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            12238..14112
                     /gene="ltrA"
                     /locus_tag="IWO04_001094"
     CDS             12238..14112
                     /gene="ltrA"
                     /locus_tag="IWO04_001094"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0212720.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            14238..14594
                     /locus_tag="IWO04_001095"
     CDS             14238..14594
                     /locus_tag="IWO04_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0212721.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            14639..15781
                     /gene="dcm"
                     /locus_tag="IWO04_001096"
     CDS             14639..15781
                     /gene="dcm"
                     /locus_tag="IWO04_001096"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0212722.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKIDDL"
     gene            15771..16060
                     /locus_tag="IWO04_001097"
                     /pseudo
     CDS             15771..16060
                     /locus_tag="IWO04_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16316..17500
                     /locus_tag="IWO04_001098"
     CDS             16316..17500
                     /locus_tag="IWO04_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0212723.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            17515..17610
                     /locus_tag="IWO04_001099"
     CDS             17515..17610
                     /locus_tag="IWO04_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0212724.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            17639..17860
                     /locus_tag="IWO04_001100"
     CDS             17639..17860
                     /locus_tag="IWO04_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212725.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            17873..18376
                     /locus_tag="IWO04_001101"
     CDS             17873..18376
                     /locus_tag="IWO04_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0212726.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            18503..19540
                     /locus_tag="IWO04_001102"
     CDS             18503..19540
                     /locus_tag="IWO04_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212727.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            19555..21252
                     /locus_tag="IWO04_001103"
     CDS             19555..21252
                     /locus_tag="IWO04_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ0212728.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            21245..21706
                     /locus_tag="IWO04_001104"
     CDS             21245..21706
                     /locus_tag="IWO04_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212729.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            21719..22579
                     /locus_tag="IWO04_001105"
     CDS             21719..22579
                     /locus_tag="IWO04_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212730.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            22617..23006
                     /locus_tag="IWO04_001106"
     CDS             22617..23006
                     /locus_tag="IWO04_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0212731.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            22993..25440
                     /locus_tag="IWO04_001107"
     CDS             22993..25440
                     /locus_tag="IWO04_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0212732.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            25445..27553
                     /locus_tag="IWO04_001108"
     CDS             25445..27553
                     /locus_tag="IWO04_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0212733.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            27550..28554
                     /locus_tag="IWO04_001109"
     CDS             27550..28554
                     /locus_tag="IWO04_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0212734.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            28573..29484
                     /locus_tag="IWO04_001110"
     CDS             28573..29484
                     /locus_tag="IWO04_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0212735.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            29671..29958
                     /locus_tag="IWO04_001111"
                     /pseudo
     CDS             29671..29958
                     /locus_tag="IWO04_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(30146..30361)
                     /locus_tag="IWO04_001112"
     CDS             complement(30146..30361)
                     /locus_tag="IWO04_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212736.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(30620..30787)
                     /locus_tag="IWO04_001113"
     CDS             complement(30620..30787)
                     /locus_tag="IWO04_001113"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0212737.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(31132..32571)
                     /locus_tag="IWO04_001114"
                     /pseudo
     CDS             complement(31132..32571)
                     /locus_tag="IWO04_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(33119..33790)
                     /locus_tag="IWO04_001115"
     CDS             complement(33119..33790)
                     /locus_tag="IWO04_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0212738.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(34139..35542)
                     /locus_tag="IWO04_001116"
     CDS             complement(34139..35542)
                     /locus_tag="IWO04_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0212739.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     AESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            complement(35654..36997)
                     /locus_tag="IWO04_001117"
     CDS             complement(35654..36997)
                     /locus_tag="IWO04_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0212740.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(37131..>38322)
                     /locus_tag="IWO04_001118"
     CDS             complement(37131..>38322)
                     /locus_tag="IWO04_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0212741.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT        12486 a         6583 c         7247 g        12006 t
ORIGIN      
        1 cttgcaggat tattattcga ttcagcatta tttttcattg cttcacgcat acgttcagaa
       61 ctagacataa ttctatcggc attttcaact ttagcaccat cttttctatc tgaaagacgt
      121 gttggcacgg aatcagcata ttttccagtt tcatcttctt tatttttagc taacataggt
      181 ttgctatttt tattaatagc ggacactgta ccatcatcta aaccttcttt aaacttattc
      241 ttggtatcat tagctatatc acgcatactc ttaaatggtt ttgttgctct ttcactagcc
      301 attgtagctc catctgaagc taaaccggat aatccacgtt catgggcata tcccaaagct
      361 cttccagttt tgccagcttt ttttgataga gtttcgccga aattcttttc tggttttcga
      421 tcttctcctt ctccgcttcc tttagcacga ttaaatccat ttgatccttt tctaaacagc
      481 attgcgccca ttccaaaagc agaagcaagt tgcccatagc cttctttgag tccaatatca
      541 acaccaaagt atctcatgac tgttccactg cctttgatta aaacaaaaac ggcacagata
      601 taagcaattg tttttataaa aatatttgtt gagatacctt gtcctgagtt cagaaaattg
      661 ataaacatag caaacattga taacccaaat ccttgaaacc caacagttaa gtaacattgt
      721 agaatatctg atagaacaac ttttgaacgc tgtcctgtct caatatctgt tgcaaaaaca
      781 agaacaccta acacacgttt aaatgcaagt tctagtattg ctgtaatgat cacaaacgcg
      841 ctgaacacat aggcgattgc taacgctgtc aacccaattg aaatccccca tagatttgct
      901 tggtatcggt aatagcctga tttcaagcta tcagaaaatg ttgataagaa gttgtcagaa
      961 aactttgtag caacaaattc attattagaa ttttcaacta attcataacg aagattgtcc
     1021 gcaattggat tttctttttc caatttatca atcactttat ccgttaacac ttgtgtgaga
     1081 tccgtctgtt gaaaattttt tcgtgttaaa ttatttcgat cctccgtttt atcaatgctt
     1141 gagtactgat ctgttttatt aatatatgct aaatctgtta ctccttgttt tatcaatgac
     1201 caagcgactc cgtcatctga atttgtaatc gtagtagagt ccttataaaa tgttttagca
     1261 aattgaatac cactagaaat catggttggc attaaaagta ttaatgctat agacatcact
     1321 acgttcattc caaccgattt taaatccact gttccttttc cagtaatcat tttgtaacca
     1381 acaaacatca atgaaaggat cattaatcca atgacaattg tatttacggc tgattgataa
     1441 acattattta gccctgtact ttcaatgaaa ttgaacaaat ccagcacatc tggaatcata
     1501 ttttgaatcg aatctgtcac agtaaacatg ccttttacca cagcccatcc gagccaaatc
     1561 ccgaactgac taaggaaatc tccattgtac ttttttaggt aaggtctata aagagaatta
     1621 ttcaaaaact ctgtttgact ttttgtgtca tcaaaccaat tgaaaatact cgcatgagca
     1681 atattacttg taaaaataat catagctatc agaacaagaa aattaacaac caaccaattt
     1741 atttttttat tggcaatctt tttttcaatt attttcatcg ttacctccct tagcccattt
     1801 tctgaggctc tcagagagct tctgagccat tttttgatta attatgacat gagttacaca
     1861 attatcattt tcagctctta aatcgagaac tatagtgttg aagcaaatag tctcgattaa
     1921 ttcgagacta tctgtatttt gatctagaca ccaaacttga tctattaaac gatttttaat
     1981 caaaataacc cctcctatat tcaaagttct caaatgaagt ggagataccc ctccatttca
     2041 tttgactcaa ttctattctt attgcttatc tattccttcg ctgatttgca attcaattag
     2101 ttctaacgct ttatttttta gagaagcaaa gatataatcg tctttttgtt tgatacgatg
     2161 atctgttttt gctcttcgta tcaagtcacg taaagtcatt gatagctgtt ctctaacatg
     2221 atctacaaaa tacttgttat tacaaatttc atagataatt ttcagttgaa ttttttgttc
     2281 tttttcagca gatctaatcg catatttgaa ttttttgtac cagttgtcga attgacgata
     2341 atcactattg ctagccagtt ttaagcaatt cacaatactt tctccccata tttgtttaag
     2401 aagattttct tcaataaatg tttcgatcaa ttcttcatct aatttattat ttttagttga
     2461 aagtgattca agaagatcat cattatgtga ttctttaata tctttactat ctttataaga
     2521 cttatataat tctagttcat tttttgaact atcagcaaaa ttgtttttgt ctcttgtttt
     2581 cattgatgtg tcgcatagtt caaattttga attatcaaca gaatattgat ttgttccata
     2641 gttcaaattt tgaactatca agtttttttg tctattttct tgatgggtta tcttattaat
     2701 tttataaaca tcatctttgc acagaacagg ctttaacaaa taataaaaat ttgggaacat
     2761 ttgcatttta cttttattga agccattttt tcttttcaca agaagtttaa ctttttgaag
     2821 ttcatctagt gctgataaga ttgtattttt tgacattccc gaaatttcta cgagattttt
     2881 gatttcaaat atcaaaaaaa ttttttgatt ctcatcatac aagttatttt tcaatgaaag
     2941 tctatttcta tcttttagta acatataaac taacttagaa cttagtttca ttttcttata
     3001 tttttcattt acgtttaaaa aaataggata ttgatagtat aattctgcat atatatcaga
     3061 attagaatag ccttgctttt caactaagct ttcaacttct ttttcattca ttgtcacacc
     3121 tgcttctgat tttatttgct ataatagatt caaataaagt tttctcagaa ctttattatt
     3181 gagtgttccc ccgatctgca aactgacggg aacactcttt ttaatttttt ctacttcatc
     3241 aaatttaaaa aacgaggtgt tttttaaatt tgatgactca atatccattt tttgcattct
     3301 tcttttgaaa acaactccat tttttctaat aaaataactg gcaatcccat tcttttaaat
     3361 ttcaaaaaag ttgtatatga tacattcaaa tatttagcca tctcttttcc ttttagaaat
     3421 ggtctttcta atccggtatc aattcttgct tgatcaatag cctcttttgt catttcaaaa
     3481 acataatttt tcaattgatt cgtttgttgt ttatctaaca ttactgtaat tcctgcatcc
     3541 attatttttc tcctctcatt ttctatttct tcaatcagcc aactcgccaa gttgtattta
     3601 tttagatttt caaatcatta agtaaccaat ttaccaactt ttgataagta gatgctctaa
     3661 tactttcttt gctcccattt tcgattttga catatgtttt ccgatttata cctatcaatt
     3721 ttgctgcatc gtttttagta agtataagat cggctctatg tcttcttaag cttctaatga
     3781 attgatcatt aagagttatc attcagcgcc tcctttcaaa ttattcactt tgtggttttt
     3841 atcttaataa gcacatttta aaccacaaaa cgagtaatat caattaaaat attactcaat
     3901 ttgtggtttt catgctataa tagtttggaa tttactatag ggggcgttac tttgaataga
     3961 ataaaatttt atattgattc cagtaaaaag accattaaac aaattagtac agaaactggt
     4021 attaactata caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa
     4081 acatggaata tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac
     4141 gaaacaaatt ccatagaaca acctaatctt gatagaataa aaactgctcg tttagaaaaa
     4201 aaggacaaaa tagtcaagaa tattgccagt cttgactcag attattttac tgaattatct
     4261 aatctatact tttccatgac ttctttactt gaagtcaaac ttcaagaaga tgactccgac
     4321 tttctttttt taaaaagtat tctagaggac tcctttgaat acacgattct atttgccaaa
     4381 caaaagaagt ttattaataa agctttaaac ttaaatatcg atgaaagaaa agagttagaa
     4441 tctgagttat ttggcaactt aacccaagta caggttaaca atctaaaaag agagataaca
     4501 gctcgattaa tatcaaaact tcaataaaac taattattca atcagccaac tcgccaagtt
     4561 atcagattgg agagattagt tatgtcaata caaaagtaca caacaaaaag cggtgcacga
     4621 tatagtgtcc gcttgtatgt tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa
     4681 aaagggttta aaactgaaaa agaagccaag ttacacgaag caagaaaaaa ggtcgagatt
     4741 gaagataccg gctatcataa gccttctaat gacttgttta taacagtgta tgaagaatgg
     4801 ttgccttctt ataggaatcg tgtacaagaa acgacatatc aacgtacaaa agaccttttc
     4861 cgcttgcata tactaccaaa attcgggaat aagtccattt ctaagattac tcctgttcac
     4921 tgtcagaatg cagtcaatga atgggcttta aaattcaaga atatcaaaca gcttaagtct
     4981 tacagctctc aaatatttga gtatgccatt tttgcagagc tattacaccg aaatcctatg
     5041 gcaaatacaa atctaccaaa gcgggaaaag aaagaaaatg aaaattactt ttcattagaa
     5101 gaactaaaag ctttttggga tattctcaat aaagaagagc ctttaaaaca tattttaatc
     5161 ttccagcttt tgattactac tggtatacgt aaaggagagc tagctgctct tcattggtct
     5221 gacatttatt ttgaagaaca gctaatgtat gttagaaaga gctatgcaac tattcgaaat
     5281 gaagagaaga atgcaagaag aaaaactgtc aggattcaaa aaaatacaaa aaatatatct
     5341 tccgaacgaa tattaccaat tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa
     5401 gaacaagctc gagagctttt gcagtttggc ataaatacaa atacaaaaaa tcaacttatc
     5461 tttacctatg taaatgcaga cggtgacatt aatcaacctt tgcatgcgga ctattcaaat
     5521 aatatattga aacgtttaga gaagaaatat aaatttaagc acgttactat tcatggattc
     5581 cggcacactc acgctactct tctactagaa ggtggagcaa gtatcaaaga aacacaagat
     5641 cgattgggtc ataaaaatgc tgaaactacc cttaacactt attctcatgt tactgaaaaa
     5701 gctcaacgaa atgcgttaga aaaattcatt gcttacactg gtttataata ttgtcattac
     5761 ctttctcatt accagaacga aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc
     5821 gttggtataa caacgattaa cgttttgaaa actgtgaagc tttacgtgct ttcttaagac
     5881 ctggtttttt cttcgtatca ttgaataagt ttgaaaagcc ttgatttata aaggtttttc
     5941 cgtaaatata ttttgttata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc
     6001 taatttaaag gcagtatatt tcgtatttta tattaaattt ttaggctcat tcattctcaa
     6061 atccattcta tttagtcaat aaatatttta aaaagacttt ataaacaaag atgttcaagc
     6121 gagcatctta tttttttgtg cctaatttga aaggagaaca tttcatgtat aaaaaacaaa
     6181 aaaagaaatt atgtgtatta actattttga gtttggctgt tcaatcaatt gtaggagcaa
     6241 ttgctcctac gattgctttt gctgatgaaa ttacccatcc acaaacggta accgtagaac
     6301 tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag
     6361 aagtcactgt tccgcattat gcagtgtaca acggcgttaa acaagatatt ttctgtgttg
     6421 aaccaggagt cccgatttac aatgagttca ctcctggata tgagaagaac ccgttaccag
     6481 atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag
     6541 atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact
     6601 caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaatcaatc
     6661 aagccattgc cgattatcag aagaaaccaa gcttccataa tagtacggct aaaacagtat
     6721 tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg
     6781 ttgtggaaaa taccgcaaac attgattatc gtgtgaatgg caatcagttg ataatcagcc
     6841 caaatgccaa ttcaaaagaa agtggtgtgt tgacgcttaa gaaatctgct ggtaccggaa
     6901 cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg atcgataagc
     6961 cgaacactta tacggtcaaa attgatgtgg aaatggaggg ttctttgaag attaaaaaag
     7021 ttgataaaga atcgggtgat attataccag gaacggtttt ccatttagac tttggaaaaa
     7081 ctttacccgc aaaagacgtg accaccgata aagaaggcat tgcgacctta gatgagattc
     7141 ctcatggtac aaaggtaacc attactgaaa aatcggtgcc ggcgccttat acgattgata
     7201 ccacacccat gactgccaca attaaagcag gtgaaaccat ttcagtgact tcaaaaaata
     7261 cgcgagaaaa aggtcaaatt attctggata aaaccggaat tgaaacaggg agtgatcttt
     7321 ggaacgataa ttactcttta gcgggcaata cgtttgccat tcgcaaggat agcccaactg
     7381 gtgaaatcgt ccaagaaatg accacggatg aaaaaggtca tgcagaaaca ccaaaagaga
     7441 ttgccaatgc gttggaattg ggaacctact atgtgactga aaccaaggct agtcatggct
     7501 ttgtgaatac tttcaaacca gtaaaagtcg aattgaatta tgtcaatcaa acagtggctc
     7561 ttgttaccag taacgtaaaa gggcaaaacc aagaaattac tggtgaaacc actttgacaa
     7621 aagaagacaa agataccggt catgaaactc aagggaaagc tgagtttaaa agagctgaat
     7681 acaccttgtt tactgttaag gatggaaaag cggtcaaatg gaccgagaac tttaaaccag
     7741 aattgttgaa gggaacgaaa gcttctgatg aaaccgtaac tttgacttta gatgaaaaga
     7801 atcaagttgc cgttaaacac ttagcgatta atgagtatta ctggcaagaa accaaagcgc
     7861 ctgaaggata taccttggat gaaacaaagt atcctgtatc catcaaaaag gtggatgata
     7921 gcgaaaaaaa tgctgtgatt actcgagatg ttgcggcaaa agaacaagtg attcgctttg
     7981 gctttgattt ctttaaattt gctggatcaa ctactggcac tgccgaaact ggcttcaatg
     8041 atctaacctt taaagtgtcg ccattggaag gaaccaatga aatcaccggt gccgaagatg
     8101 aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc
     8161 ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta
     8221 cgccattaga aatccgttct acttttaaag aaaataaaga ggattatacc aagagtgatt
     8281 atatctttac tattaccgaa aaagggcaaa agcaaccgat taaaacggta acggtccctt
     8341 atgggaatct aaccaatgag aatttctcag tcagtttgaa ccgtttgatg ctttatgatt
     8401 tgcctgagga agaagatagc ttaacttctc ttgcgacttg gaaagaagga gataaagaat
     8461 tgactactct tgattctacc gagctagtcg ataaattgag ctataatcta catgaaatca
     8521 aagaagactg gtatatcgta gctcaagcca tcgatgtaga tgctacaaaa gctgcccaag
     8581 aaaaagataa aaaagccaaa ccggtggcga ttgcggaaac aaccgcaact ttagcgaata
     8641 aagagaaaac aggaacttgg gagattctgc ataaattaac tgtcgaacaa gttttagaca
     8701 agaccattgt cttattcaac tatgtgtatg aaagcaaaga agcctatgaa gctggtgatg
     8761 agccggtagc gaaggatgct agcttgaaca atcaagcgca aactgttaat tgtatggtgg
     8821 aacgtcatgt ttccatccaa accaaagcgc acttagaaga aggttctcag acctttactc
     8881 atggtgacgt ggtagatatg tttgatgatg tatccattac ccatgatgta ctagatggct
     8941 caaaagaagc ttttgaaaca attctgtacg ctttatttcc agatggtaca agtaaagaaa
     9001 tttggaaatc tggcaagatt gattacgaag tgaatgacaa agaatttacc aaaaccgtac
     9061 ttgcggaaaa agtggataca agtaaatatc cagaaggtac aagttttacc ttcaaagaaa
     9121 ttaactatga caaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat
     9181 ctcaaacttt aacgcccaaa aaagttccga caacaccaag tacaccagaa caatctgaaa
     9241 tgccaactgt tccaagtggc tctcaagaat ctagtcctac agtgaaggca ttccctcaaa
     9301 ccggggagaa aacttccaat gtgctactgt tcattggctt tagcttgatc tttgcgacgg
     9361 ctggctatta tttttggaat cgtcggaact aaggtgataa gatgaaacat gtaaatagta
     9421 gaaaacaata cgcgcgaccg cctcctaaaa aagtggtcgc gctatttttg tattcaaaac
     9481 taacaatgaa aagaggaaaa aatatggaat tgaaatttgt tgtaccagat atggtcgaga
     9541 cgttcggaaa actaacatac ggtggtgaag gtgaagttct atcggaaggg tatggtcgaa
     9601 atactacaat tgttggtcga agttaccatc tgttttcaaa taaacaacgg gccgatgata
     9661 ttgaagtggt ggtagctgcg gaagccggcg aaaaggattt tgaccaagat caaccactaa
     9721 aagctgtaaa tccccactta attgccaaag gctatgaaat tgagaatcgt gggtttaccg
     9781 attacgtgtt gtatgttgat gatttagtga aagcatagga ggaaaagaag atgagattaa
     9841 cagaaggtat tgtcgtagat tcaggattaa cctttggcaa gttgcgtttt tccgcattac
     9901 gtcgagaagt acgcaagcaa aatgaggacg gaacggttag taacgaggta aaagaacgga
     9961 cctataattt gaaatcctcc gcgcaaggtc gaatgatcca agtcagtatt ccagcaaatg
    10021 tacccttacg cgagtttgct tatgatgcag aggtagagct ggtcaatcca atcgtggata
    10081 cggtcgccaa ttatgttttt cgggaaggaa ccaccgttaa ttggtttatc aaggcagatg
    10141 atttagtctt gaaacgacaa ctgaatcaag gaaaacctac gaaccaaaat gaaggaaaga
    10201 aataggtgaa gaatatggaa attaatgttg aacatatttt agattgtctc gatcaatatg
    10261 gaaaaggtga gctaacggag gaacaactca ctaacgcatt aacctatgat gaaaaaatgt
    10321 ttctgattat gcatcaaggg ttactggaag taggtaacga tgccaaagaa gactttgata
    10381 taccggattg gttaggagaa gaagagtctt tctttatggt tgtcgaggta aatgaaagtt
    10441 tatgtcgaca ggcagagtct gtcttggaag aaatcggtgt ggagatgccg gatgccattg
    10501 aaggcttttt aaaacaattg gtggaaacga aacaacttcc cgtagtagta aatgactagc
    10561 ataacttttt gaaattaccc aatgaaatcc tattgggctt tttcgactgg aggtagaaat
    10621 catcaaaatg tacaaaggac atcgcatacg cgcaggagat caacacttga tttatcactt
    10681 tgttcttggc tggctaagtg ccttattcat cggttggatg ggcgtctttt attttccaga
    10741 gcttagacaa tttggtcttt ctaaggtatc attttccact attgaaatcg cttggtccat
    10801 gaaagattta gtatgtctgc ttggaagtct cgctctttca ggaagtatga tgttgcttta
    10861 tattcatttt tttcctgatc attggcgaag tttgtggaat cgacaaaagc tagccagaat
    10921 gattctagaa aaccattggt acgaagtgaa gcaaactcaa agtgatggct ttttcaaaga
    10981 tttgaatagg agtcgaacca aagagactat cagttacttc cccaaagttt attatcgaat
    11041 gaaagatggc ttactgtcta ttcgcgttca aatttcactg ggaaaatatc aagatcagct
    11101 attgaagttg gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact
    11161 caaagactcc tatgtggaat acaccttgtt atacgatatg attgccaatc gaattgggat
    11221 agaagaagta gtggcagaaa atgggacgtt gcgattgatg aaaaatcaag tatgggcgta
    11281 tgattccttg cctcatatgt taatcgctgg tggcacaggt ggtgggaaga cctatttctt
    11341 actcaccatt attcaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa
    11401 aaatgcggac ctcgccgatt taggtacggt aatgcctcat gtctattctc aaaaagaaga
    11461 aatttctaaa tgtgtggaag atttttacga acgcatgatg gctcgtagta ggtcaatgaa
    11521 agaaatgccc aactacaaaa caggagagaa ttatgcgtat ctcggacttc cgcccaactt
    11581 tttaatcttt gatgaatacg tggcttatat gggtgcgaat cgttttccta caagcatcga
    11641 atagatgtga aatggaagcc tattgagatt atcaatagtg acaactaatg ttttgagaga
    11701 tggaatgaaa gccttcaagt tgagctgaaa catccccgct aatactccta catgctctaa
    11761 tcaatgaagt agattggttt tggtatgaag ctaggtgaag tcgtctgaaa tctgcctaag
    11821 tgcttgagca taaggctgac tatagggcgg gcggctgaaa aagataacta tggtaagaat
    11881 gtctgaaaat gactgacgaa tctgcgaatg tacgggtcta aaaggctgat cttattgaaa
    11941 gataggaaca tacggacgta tggtgagata ccgacaagta aggttccatg atatgaaaat
    12001 cgggatagct aacgaagaag ctataatttc acaggctcat agtagatacc taaggttata
    12061 catgatagct aaaaacattg gaacgatgta agcagaaaac tatcaattaa atgactactt
    12121 ctagataggt gcatataagt aataacgaaa ttgctttgtt tctgtgaaag taagggcaca
    12181 gtaccgatga agcgtctaac aaatgtggag ggatagccct aagtctattt aataacaatg
    12241 aatgttgaaa cagatgaagt tcaaggatct ggtaagaacg tgggaaaatc cgttatggag
    12301 gtgccacgct tgaacacaaa aatacgttac tgggaatatt atgatctaca agaaacattc
    12361 gatcagctat ttactcaaag tagaaacggg aaaaagtttt atcaattata tgagttaatc
    12421 atttctgaaa ataatattct tttagcatac cgtactatca aggcaaacaa ggggtcttcg
    12481 acaccaggaa cggattcctt cacaattgat aactataagg aaatgaacca agcagagttt
    12541 atccatctta ttctcagtca tttagaaaat tacaaaccaa aatccataaa gcgagtaatg
    12601 atacctaaac ccaatggtga gaaacgccca ttaggaattc catgcatgat tgatcgaatg
    12661 atccaacaga tgttcaagca gatactggag ccaatatgtg aagctaagtt ttatgaacat
    12721 agctacggtt tcagaccatt gagaagtgca aagcatgctc taggtcgcat tatgtatctc
    12781 ataaacatca gtaaaatgca ctatgcagta gacattgata tcaaaggttt ctttgataat
    12841 gtaaaccacc gtttactgat aaaacaatta tggaatatag gaatttgtga caaacaagtc
    12901 ttagctattc tttcaaaatc tctgaaatca cccattcaag gagaaggtat cccgagcaaa
    12961 ggaacgatcc aaggaggtat catttctcca ctattatcta atgttgttct aaacgactta
    13021 gaccattggg tctcaaagca gtggcatacc tttgaaacaa agtatcccta tactaaaggt
    13081 tacaataaat ttcgtgctct aagagataca aatctaaagc aaggctatat tgtcaggtac
    13141 gcagatgact ttaaaatcat gactaatgat tatccgacag cattaaaatg gttccacgct
    13201 gtcaagcttt acctaaaaga cagacttaaa ttagatattt ccaatgagaa gtctaaaatc
    13261 gtcaatttgc gtaaatgtaa gtcggaattt ttaggattcg ctatttgcgt gaaacaaaaa
    13321 gggaaaaaat gggtttgtaa ttcacgtatt tctaacaaaa aaaaggatca gattaaagaa
    13381 gagattaaac aaagaatcaa agatattcaa aagagtccta cagcacaaaa tgctctcctg
    13441 tttaatgctc tcgtgctcgg tgttcataat tatttcaggt atgccactca tattaattta
    13501 gatttgaata ggatgaacta cgttctttca ttcactactt acaaccgatt atcaaattgt
    13561 agtacaagat gttaccccaa caaagcttcc ccaacttaca aaaagtttta ctcaacaacg
    13621 atgaaaacgt ataaagtagc tggagtctat ttgtaccctc tttgcgacgt atctacaaaa
    13681 acaatttacg gatttaatac ggaggataca ccgtttactc cgtttggtcg tcaaagattg
    13741 gagcacaaaa gcctacagtc tcttgtgttc caagaattaa gaaaattgat ggaatcgaaa
    13801 ataccgaata gaacggttga gtatttagat agtagaatat ctcgttactc aatgaaatca
    13861 ggcaagtgtg agattacaaa acaatttttg ccagcaaaag ccgttcactg tcatcactat
    13921 ttgccaaaaa gtcttggtgg agacgacaaa ttcgataact tgagaattat tcataaggat
    13981 atccatcttc tgatccatac aacaaataaa atgataatcg atcactacgt gcatgaactg
    14041 aaactggtgc cagagcaaat agcaaaaatc aacctttatc gaaagatgtg taatctgcaa
    14101 aacattcaat agaaaataaa tagatggaac gccgtatgag gtgaaagtct cacgtacggt
    14161 gtggagcggg ggaaaagatg gagattatat caaattctta cctatcgcta tagatgttaa
    14221 cgacaaaaga aagtgcggtg attttgaata agcttaaaca aatcgtgatg ttaggccgtc
    14281 aatctggttt ctttctgatt ctagcttgtc aaaggccgga tgcgaaatat ctaggagatg
    14341 ggattcgcga tcagtttaac ttccgtgtgg ctttgggtcg tatgagtgag cttgggtatt
    14401 ccatgatgtt tggagaagtc gataaaaatt tttttatgaa acgcatcaaa ggacgaggtt
    14461 atgtagatac agggggcagt gtgattagtg agttttatac gccacttgta ccaaaagggt
    14521 atgatttctt aagggaaatt agcaacattg ttggattaac cgttcatacg gaaagagaaa
    14581 ataatagtgt gtagcctagg aatatcaata atcctgggct tttttattga ggtgatttat
    14641 gacattttta gatttattcg ccgggattgg tggctttcgc ttaggtatgg aacaagccgg
    14701 tcaccagtgt atcggttttt gtgaaattga tgaatttgct cggaggagtt ataaagcaat
    14761 ccatgataca aggaaggagg tggaaatgca tgacatcaca agtgtatcag acgaatttgt
    14821 tcgatccctc ggaccagtgg acatcctttg cggcggcttt ccgtgccaag ctttttcaat
    14881 tgcgggaaag cggcaaggat tttccgatac tcgaggtact ttattctttg aaatcgctcg
    14941 gttcgccact cttctccaac ctaagtttct attccttgag aacgtccggg gattgctcaa
    15001 tcacgaagga ggggctacgt tcgagacgat cctccgaacg ttggatggat tggggtatga
    15061 tgtggaatgg caagtgctta actcaaaggc ctacgttccc caaagccgtg agcggatctt
    15121 ccttatcgga cattctcgag atgcatgtac cgaacaagta tttcctatca ttggatcgtc
    15181 ttcaacatct gatcaaaaca tcagaaactt ggtaaatatc aatccttcca atcgaggaat
    15241 gggtggccaa gtctatggtt cagatagcgt agcaccaacc ttgaccggtg atgaaggaat
    15301 taaaattgcc ttaccggtaa aggatgggat ttctgtagcg ggtatgttgt cgggaaattt
    15361 tgaacaaggc aataggattt atgaggtaac cggaacagcc cccactttgt caacgaaaca
    15421 agggggaacg aagattatga ttcggaaaaa tgaaacagaa tatcaagagg tgaagccagg
    15481 ggacagtgtg aacctggctt ttcctaattc cacaagtcgc cgaggtcgtc tgggaaaaca
    15541 aagtgtgcat accttactaa caggagacca acaagcggta gtcacggatc agtaccagat
    15601 tcgaaagtta acacctaggg agtgctggcg gcttcagggc tttccagatt gggcttttga
    15661 tcgagcttct caagtcaatt cagatagtca gttgtataaa caggccggaa actcagtaac
    15721 agttccggtc atttttgata ttgctagaag attaaagggg gtaaaaatag atgatttatg
    15781 acgagttgat tggagaaatc tactgggtga ttggcaagat tcaggcagat ccgaaattgg
    15841 aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa gtccaggtga
    15901 ttcgtatgta gtggatgagg aaacggatgc tcgaatggag ctcaatcaag tcatcggtga
    15961 attggaacga attgcggacc ttgccaaaga acctgatatc cgccaatact tgtttgagat
    16021 aaaagcagag ttggaaattg agggtatcac cgcggagtaa tagggaaata acccactgat
    16081 aggcactgac cgcgggggat ttgcgaagca gaaatcctcc gtggtgctgt gcctgttcat
    16141 ggacggcggc agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac
    16201 acaaaaaaca agcaagtaac cataaaaaat gcttgttgta tcaaggatat taagtaattt
    16261 gtataggtgt taactcattt ggaaaagtta acaccttgaa tttggaggtg aaaacttggc
    16321 gcaacgaaat ttagattatc ggttattaaa agaacgacgc aacgaatatg gcgtttcaca
    16381 aaataaatta gctactgctt gcggacttag ccgcccatac ctaaatcaga tagaaaacgg
    16441 tggcgtaatc gcctctacaa agaccatgag aaaaattttt gatcaactgg aaagtttcaa
    16501 tcccgattta cctttaacgt tactatttga ctatgtaagg attcgctttc ccacaacgga
    16561 tgtgcggaaa attattcaag aaattctcca tttgaaattc gagtatatgc ttcatgaaga
    16621 ttacgccttt tactcctatc aagaacaata tgtcatgggc gatattgtag tgatgttatc
    16681 tcatgaagaa gataaaggtg tccttttgga attaaaaggt cgtggttgcc ggcagtttga
    16741 aactttttta ctcgcccaaa agcgtagctg gtacgacttt tttgaagatt gtctaaaagc
    16801 cggtggcgtg atgaaacgct tggatttagc aatcaatgat cgagtggggc tactggatat
    16861 tcctgattta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa
    16921 aagttaccgt tccggggaac tgttgaaagc tgatgaaaag gatggtatgg gcaatacctt
    16981 atatattggc agcctcaaaa gcgaggttta tttttgtttg tacgaaaaag attatgaaca
    17041 atatatcaag ttagggattc cattggacca gaccgaaacg aaaaatcgtt ttgagattcg
    17101 gttgaaaaat gaccgtgctt attatgcgat tcaagatttg ttaaaaggtc gtagcattga
    17161 aagtaccacg ttttccatta tcaatcggta tttgcgattt gcggataaag tagatggaaa
    17221 acggcgaacc aattggccct tgaatgaaca atggggtcgt tttattggac ggaatcggaa
    17281 agagattcaa ttaacttctg aaccgaaacc gtataccatt gaacgaacat taaattggct
    17341 aggtagacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcaaac
    17401 aacgtatatc caggatatgg tacagaatgc gcgactctcg gaccggcata aaaagatttt
    17461 ggagcaacaa agtatggcaa tcgaaaactt gattgtatga aaagaggaat tttgatggaa
    17521 attgtaaaag atatgttatt ggtacttggt ggcagtatgc ttggtgttac cgtgatgtgc
    17581 ttgatgaatg caagtgcagc agcggattga gcaatggaaa agaaaggaaa gtgacaaaat
    17641 gaatttcgga caaaatttat atcaatggtt tttaaccaat gctcaatctt tagtcttact
    17701 agcgattgtg gtcattggtt tgtttttagg atttaaacgg gagttttcca aactgattgg
    17761 cttcttggtg attgctttga ttgcggtagg tctggtcttt aatgcttctg gtgtgaagga
    17821 tgtcctactc aatttgttta accgaattgt cggtgcctga gggggaatca caatggaaca
    17881 gatgcgtgtt tatattgcga accttggaaa atacaatgaa ggtgagttgg tgggggcatg
    17941 gtttacccca cctgtcgatt ttgatgaagt gaaagaacag attggattaa atgacgaata
    18001 tgaagagtac gcgattcatg attatgaact gccttttgag attgatgagt atacgccaat
    18061 tgaagagatt aatcggttgt gtaacttagc ggaggaacta gaaggaacgc caattggtga
    18121 agctgcttct gagattcaac acgctttctt caattctttc gaggaaatgg tagagcatgt
    18181 ggatgatatt gtctattatc cagattgcga tgatatgagt gatgtggcgc attacttgat
    18241 tgtagaagct ggtgattttg gcgaagttcc agaacaattg aagccgtata ttgattttga
    18301 tgcttgcggt cgagatttgg agattggagg aaattatttg gttactagtc gtggagtttt
    18361 tgagtatcca gaatgaatca aacactatat atagtgtttg attagtatat gtaagcaaaa
    18421 aaacacaata tatagagtgc aaactgaagg gtaatgtgat ataatatttg ccgaagaaat
    18481 atttttgaaa tgaggtaata ttatggcaaa tattcaaaca agttttattg atttccataa
    18541 ttcaattaga ttggacgtag aagacaatac gctacttaag gactataaag accaagtaat
    18601 tgatggatta aaagattact tacctgatga tgtgaagttt gaaacttttt tacagggaag
    18661 ctattctgtt tataccggaa taaaaagctg tgatgaaaaa attgattttg acatcgatat
    18721 tgcagttgct ttcgaaattg atcatactgt ttatgaagat ccaagggagc ctaaactgtg
    18781 ggtaaaagag gcgctagttg aaatttttcc taatgctcaa gttaatttaa aagtaccttg
    18841 tgtaactgct acatttactg gaaaaaagac aaaaaaaaat gtgcacgttg atgttgcggt
    18901 ttatgctaaa gaagatgaaa attatttctt agcaaaagca aaagaattca gtgctcccga
    18961 aaaccgatgc tgggaggaag cggatcccaa agtattaaaa gagaaaatta atagtcatgt
    19021 agcagattca gatgatcgaa aacaatttcg aagatgtatt cgctatttaa aaagatggaa
    19081 agataacaat tttaaccaag aatataaacc aacaggaatt ggacttacaa ttaatgtgat
    19141 ggatacgttc ctagttaata agtccactga ttttttaact agaaaagttc agtataatga
    19201 tatggaatgt atgaagcaga ttgtttcttc gctgaaagat tcatttgtgt atgagtacag
    19261 tgaaacagat ggttggcatt atcgtctaca tgcatatctt ccagtgaagc ctaacagtga
    19321 cacctattca aaaatgacag tgaatcagat gagtgatttt aaaaacaaac tttctaaatt
    19381 atatgatgat ttgattttcg ctatcgatac tgaagatgag tatgaagcta caaaacgatt
    19441 aaataatcaa tttggggagg attttttaat tatttcagaa gaagaagtta cagaaaaaaa
    19501 cttaagaaat gcatttgtta cagactatcc gagtgcttag gttggtgtta aagattggat
    19561 tatactgata ttttaaaaga aaagctaaca gatgttgtgc gtttattcga tggtaattac
    19621 tatggggatt tttcatttgc tacaattgga aagttgcgtt taaaaattga aataccaaaa
    19681 cattttccat tggtacttcc taggatttac attcataatt ttgatcattt aggactttac
    19741 ctaccacatg tagaaaaaga tgggttggta tgtttttctt cagatataaa ttatttagta
    19801 gatgaagaaa attcagaatt ggttttattg cagtgtgtta ataaaactat aaaaacaatt
    19861 gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa aagagtttgt tgctttttgg
    19921 gacaaacaag agaatcaaat ttcgttattc tacctttgtg aaccagcgtt gtttatcaag
    19981 tcagttgata caataatgta cggtaagtct ttggttgctt tggataaaga gaacgaatta
    20041 caattaaatg cagcgagtag attgtttaat ggagagaaag aagatactca tgaattagat
    20101 gcatactatg ttccaattag aaacaagaat aatattatgc ccccgaagaa taataaagca
    20161 tggtcaaaga aggatatttt acaaatagta aaaaaaaatg taacaggtgc aaataaaaga
    20221 ttttttttaa aacttgaaaa aagaaagaat agacataatg ttttacttat tctagatatc
    20281 ccaattgaag aaaagtcaaa taatgtcata attggatttc tttttacaaa acaatctgga
    20341 tctataaaag attataaggg tacgcctagg actgtattag ttcagcgtca tgatgaatta
    20401 tatttggtga atagaacgag cggagaaaac tatggattgg gtgctaatat agctattgtt
    20461 ggggtagggt cagttggcag taaaattgct gaaaatcttg gtccattagg tgtgagaaag
    20521 atgacactca tagacaatga ttgttttcaa gctgaaaatt tgtatcgaaa ttcacttggt
    20581 gcagatagtt taaaaaatgc ccgatctcag aataattttt ttaaagttga tttaatgcag
    20641 gaggagctcg aaaaaaaatt cccatacctt tgtgttgaag tggagtcaat gaacgtttta
    20701 gacttaatta gtatgcaacc tggttttttc aaggaatatg actatgtttt tgtttgcata
    20761 ggagatacaa tggcaagcct ggctttaaat aattattttt ccaaaagtag tcaaaaagtt
    20821 ttctattctt ggctggagcc atatggcatt gggggacaca ctctatttat aaattattct
    20881 ctaaatcaaa aaggttgttt caattgtctt tatacagatt caaactctaa aaataggtgt
    20941 tcaaatagag cttctctggt aaaagaagga caaatttttg aaaggacttt agcgagttgc
    21001 cgctcaaaat ttataccata taatgctatt gcctcgacgg aatgtgcaat taatgcaata
    21061 aaacttttcc ataaagtcaa tcaaaataat atgaccaata gtactctttt ttcatggata
    21121 ggagatggga gtacttttga gaatttagga tatcaatata gcgacagaaa tacttttctt
    21181 aatggagtca ataagtttga agaaagattc ttttctaaat attgcaaagt atgtggggag
    21241 ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa gtaagggatg aagttcttaa
    21301 gatatttgaa caatatcggc agggaaagga tgacttgcct gaagctggag gagttcttca
    21361 tggaaaaaga cttaagaatg ggagcataat aattaccgat ataacaacac ctcaaatagg
    21421 agatatacaa aaaagattac tctttaaaaa aaaccgagaa attcatcaac agctttctaa
    21481 tgagaaatgg atagaatcaa ataagttttc tattgcattg ggagaatggc atactcatcc
    21541 tgaagctgta ccaactccat caaatgttga taagaaaagc tggagactga acgtgagcaa
    21601 gcaacatgat gatagagttt atgtttttat tatcgttgga ttttctgatt tgagaatgtg
    21661 gttactgtca aaaaaatcag atattaggca ggctataaaa ctataaagga gtgattaggt
    21721 gaaatcacaa aagaatattt ggtggtgggg gtttttagta ggaaaggtaa tgataccagc
    21781 ccttctctta atttctctac cgatgattac ttttttcttt accaataaaa aaatgagtaa
    21841 agaagccgtg atttttttct ttggagacca aaaagctacc tttattgaaa cgttagtaac
    21901 atctcttcag cttaatttga attacatttt ttcaattatt attgttatat cgttattgaa
    21961 tttttttaaa aaaagaaatt caggaaaggt gtttaactct aatggaaatg tgtactacaa
    22021 ctatttttat tttgtatttt gggtagcagc aactttactt ggttatgata aaattcagat
    22081 tgcaggaatt cctatacaca tgcaatataa gttggttttg agtggaattt tttctgaggt
    22141 gttgccagat atatacgatg atcattacga cagtgatgga acatgtaaag taagcattga
    22201 aaaagaaaat ttcgatgaca tcgatggata tgactctgtg aacttactga ttattgatac
    22261 ttacgatata aaaatgagtg aattatctat ggaaaatcaa acatatccta cgataattgt
    22321 tagagggaat tcaatagatg gagttcgtaa agttaataga tctttaatct tggaaataaa
    22381 aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa gtatttgttg cttcaacatc
    22441 taatccaaaa aatagtatca atattattaa ttcatcgttt agatttttcg gtcgatcacg
    22501 taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc aatggaaagt atagtaagaa
    22561 atatagaatt tttatttaag aaggaatttt tccttctttt tattttggag gaaactatga
    22621 aaaaaataaa aagctacacc agtatttggg cagttgaaaa agtcatttat gcgattaatg
    22681 actttcagct gccttttcct gtgaccttta accagatggc ttggtttgtt ttatccttga
    22741 tgtttgtcat tgtatttgcc catgtgcctc cgctatccat gatagagggg gcttttttaa
    22801 aatacttggg cattccgata gcggtcactt ggttcatgtc gcaaaagacc tttgacggta
    22861 agaaaccatt gggattttta cgatccttta tcagctatca cttacggttt aaggtcactt
    22921 ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga cgaaccaatc accttggtaa
    22981 ggagtgtgaa ttatgtaccc gattaagtat attgaaaata atcttgtctt taaccaagaa
    23041 ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt actccttttt gtcacctgaa
    23101 cagaagtatc aagtacatga taactttcgc caattaattg cgcaaaatcg tgaaggtaag
    23161 atccatgcct tacaaattgc cacagaaagt agtattcgtg ccacacaaga acgttcgaaa
    23221 aaggaaatta ccggtagact caaagaagta gcaaagcaac gaattgattt acagacagat
    23281 gcattagtat ccatgattgg cgatagtcag attgattatc gcttctttat tggctttaaa
    23341 ctgatttcga cagatgaaga ggtcaatcta aagaatctca ggaaatcttt cttttctgga
    23401 cttcaagaat ttgtctatgg agtgaatcat catttgatgg gtgattttgt ttccttatcc
    23461 aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa tggaaagtaa attggctcga
    23521 agatttaaag tcaaacgagt aacgccgagt gacttgacgt atttaattga acatatctat
    23581 ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc taccaaagaa aaagctgaaa
    23641 tcagaaacgc tggtgaaacg ttacgactta ttacgtccca ctcgttgttt gattgaagaa
    23701 aagcctcggt acttacggat ggaacatgaa aatcatgagt cttatgtagc gtacttaaca
    23761 attaatacca ttgtagggga aatggagttt ccttcctcgg aacttttcta ttaccagcaa
    23821 caacaattta cgtttccgat tgatacttca atgaatgtgg aaattgtgac gaataaaaag
    23881 gccctagcta ccgttcgtaa taagaaaaag gaattaaaag atttagacaa ccatgcctat
    23941 caatctgata atgaaacgaa ctccaatgta ttagatgctt tggattccgt ggatgaattg
    24001 gaaaccacct tggatcagtc gaaagaatcc atgtataaac taagctatgt ggttcgagtg
    24061 agtgctaaat cagtggatga attaaaacgc cgctgtgatg aagtgctcga tttttatgac
    24121 gataccaatg taaaactagt ccggcccttt ggcgatatga tgggtctaca caaagaattc
    24181 ttaccctcaa gtaagcgata catgaatgac tatattcaat atgttacgtc tgactttttg
    24241 gcgggacttg gttttggggc tactcaaatg ctaggagaat tagaaggcat ttactttggc
    24301 tataacgtag atacggggcg caatgtttac ctgaaaccgg cattggcatc acaaggggtc
    24361 aaaggctcag taaccaatgc tttggcatct gctttccttg gttcgttagg tggggggaaa
    24421 tcttttagca acaatctctt agtctattat gccgtccttt ttggtgggca agctgtgatt
    24481 gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt tgccagaaat tgcggatgaa
    24541 attaatattc ttaacttgac cagtaagcca gaaaatcaag gcttacttga tccctatgtg
    24601 attatgaaga aaaagaaaga ctcagaaagt ttggcgattg atatcctgac ctttttaaca
    24661 gggatttcca gtcgggatgg ggagaagttc cctgtgttac gcaaagccat tcgaacggtt
    24721 acccaaagtg atcagcgagg attattacaa gtaatcgttg aattgcgtaa agaggactca
    24781 ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta ccgattatga ttttgcccat
    24841 ttgcttttct cagatggttc gattacccag tcgattagtt tagataagca gttaaatatc
    24901 attcaagtag cggatttggt tttaccagat gcagaaacca cttttgaaga atataccacc
    24961 atggaattgc tgagtgtttc catgctaatt gttatttcca cttttgcctt ggactttatc
    25021 catagcaacc gagaaatttt caagattgtg gacctggatg aggcttggag ttttcttcaa
    25081 gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg ctggtcgggc catgaatgcc
    25141 ggtgtttact tcgtcacgca aaacagtgat gacttgttgg atgaacgttt gaagaataat
    25201 attgggctaa agtttgcatt tcgcagtacc gatattcatg aaatcaagaa aaccttggaa
    25261 ttctttggat tagataaaga agatgaaagt aatcaaaaat gcatgcgaga tttggaaaat
    25321 gggcaatgct tgatgcagga tttgtatggt cgagtaggtg tggtccaagt tcatccagtc
    25381 tttgaagaac ttttccatgc ttttgatacc cgtccgccag ttcaagagag aatggagtga
    25441 ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt ttaatcagtt tggtcactct
    25501 gataatactt ttgagcttag ttggaacggt agctgaggca acaggtctgg ttgatgatac
    25561 agttggagcc ggaaatcttt attcccaata ttcacttggt aactatcagt tggatttctt
    25621 tgtggatagt tcgtgggatt ggctgccttg gaattggggt gatggcctag ggaagagcgt
    25681 aatgtatgga ctttatgcca ttactaattt tatttggaca gttagtctct acttatcaaa
    25741 tgccacgggc tacgtggttc aagaagccta taagctggat ttcatttcag atacggctga
    25801 aagtattggg aagaatatcc aaaccttagc cggcattacc gaaaatggac tccagacctc
    25861 gggcttttac tttgggtttc tattattgat gattttagct ctaggagttt atgtggcgta
    25921 tacaggatta ttaaaacgag aaactaccaa agcagtccgg gctgtcatta attttgtgat
    25981 gatttttcta ctttcgggtt cgttcattgc gtatgcgccc acctatatca caaaaatcaa
    26041 tgattttagc tcagatgtca gcgaagcggc tcttagcctt ggaaccgaga ttgtcgtgcc
    26101 gaattcagaa agccaaggaa aggacagtgt ggatttaatc cgagatagct tgttttccat
    26161 tcaagtccaa caaccgtggt tactcttgca atttgatgat tccaatatag aagaagttgg
    26221 cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat gaaaataaag gcaaggatcg
    26281 ggaagaagcg gtcaaagcgg agattgaaga taatgacaat gcgaatctca gtattaccaa
    26341 gaccatgaac cgcttaggga ccgtggtctt tttggtgttg ttcaatattg gcatatcctt
    26401 ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa attctcttta ttatctttgc
    26461 gatgtttttg ccgattagtt tcttattgtc catgttgccg acttatgaga gtttgggcaa
    26521 gaaggcgatt attcgcttgt tcaatacgat tatgatgcga gcaggagtta ctttggtgat
    26581 tacgactgca tttagcatct ccacgatgtt tttcaatatt tcggcaacct atccattttt
    26641 catggttgct tttctgcaaa ttgtgacctt tgccggtatc tatttcaaat tgggcgacat
    26701 tatgaatatg ttcaatctcc agagtaatga tagccaatca atgggaagac gggtcatgcg
    26761 aaaaccgcaa atgttaatga atcgaacatt gcggcaactc aatcgaaatg tgggacggac
    26821 tttggctttt ggtggtggtg cagcagttgg aaataagttg gcaaaggaac aatcaaaatc
    26881 caaatttaag ccatccggtt cttctctacg aaagaattca cgattaccga acgatcacga
    26941 gataccatct gacttgaata aggaaaagcc aattccaaat aataagaaac aatcccgaat
    27001 gaatttggcc gggagaaaag tcggcaaggt tctggatacc caagctttgg tgaaagataa
    27061 gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc aacttgaaat acaacttcca
    27121 taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt aagcgaggac tggtccaaga
    27181 aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa cgtagagacg aaaaaatagc
    27241 tgaaaagcgc aaagtattga acgaaatagg gaatcctcat gggaaaagaa gggaaaatgt
    27301 tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca ccaaagcgga ttgtgagagc
    27361 aaattcgaat cccgaaatca aacgaaaatt cgctagccaa gaaatcattt caaaaggaat
    27421 aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg aaacggcggt ctactctgtc
    27481 caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct catcccaaac caaggtcagg
    27541 tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt taactttcct aacaatctct
    27601 tttatgggta tcttgatgtg tgtgggctta ttgtttggtg aagacagtga aggaagtagt
    27661 agttcaggtg ctcctcctgg ggggagtagt gtctcagaag aggttttgaa acaccgtgca
    27721 atggtggaaa aatatgccaa agaatcggat ataacagagt atgttccgat tcttttagcg
    27781 attattcaag tggaatctgg tggcacaatg gaagatgtga tgcaaagttc tgagtcagcc
    27841 ggtttaccac cgaattcatt aagtaccgag gcatccatca aacaaggatg tctctatttt
    27901 gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc aagatagtat cattcaggcc
    27961 tataactttg gcggtggtta tttggactat gtggcaaaaa acgggaagaa gcatagtttt
    28021 gctttagctg aatctttttc aaaggaaaaa tcttgcggtc aaaaaatcga ttaccctaat
    28081 cccatcgcga ataaggaaaa tggtggatgg cgctataact atggcaatat gttttattgc
    28141 ttattggtga agcaatacct aactacaaca cagtttgacg ataaaacagt ccaagggatt
    28201 tttgacgagg cgtttaagta tgaaggaacg gcttatgtat ttggtgggtc aagacccgaa
    28261 acaggatttg attgtagtgg cttgactcaa tggtgttatg ccaaagttgg tctgaaactt
    28321 cctcgggtgg ctcaagatca gtatgatgcc atgacccata ttgacctaaa agatgccaaa
    28381 ccaggggatt tggttttctt tcattcgact tatgatgccg gtacttatgt gacccatgtc
    28441 ggtatttatg caggggagaa tcgcatgttt catgcaggag atccagtagg ctggacgaat
    28501 ctaacagaaa gctattggca gcagcatctt atcggtgctg gacggtacaa ataagagaaa
    28561 agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa aggaaaagag cctaaaaaag
    28621 aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt
    28681 ttattatctt gtagtcttac ttttggcgtt tataaaaatt tcaccgccat tgatcaacat
    28741 acaactcatg aaaaagtggt tattaaagag aaagtagtga atacttctgg tattgaaaac
    28801 tttacgaagg atttttcgaa agaatatttt tcgtggaaaa ataacaaaga agcaattgaa
    28861 aagagaatga acaacctcga acagtacctt acagaagaag gtttcgcttt aagtcaagat
    28921 atggttcgag cagatattcc cactaattcc gaagttcagt cagtaaaagt cttagatgtt
    28981 gaaaagagct cggaggaatt tgtcgtttca tttttagtgg agcaaaagat tacagaggga
    29041 aaaaaggcac aatcgatttc ttctgcatac cgagtgacga tctttgaaga tgaaaacagg
    29101 aattatatag taacgagttt gccaactatg attgcaaaac ctgatagggc aaaatacaag
    29161 acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa ctacagaaga aatcgctgag
    29221 tttttggaaa cgttctttaa attatatcca tcagcttctg aaaaagaatt ggagtattat
    29281 gttgaaaatg atgcgatgcg accaatcaat attgacttaa agttttcgaa gttagaggat
    29341 ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta caaagataaa ataccttgat
    29401 aatgtatcaa aaaccactag ctactttcag tacaaattaa tattacaaaa aaatggaaac
    29461 tggaaaatca ttgatgttaa ataataatga ctcaatctaa atttatgaga agttataagt
    29521 agtttaactt atttcaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt
    29581 cggtaactcg aaaaccgaca attaagacat agtgcctttt tattttaatc catgtcataa
    29641 tcagtctata taagaaatgg ttgatatacc atggaattag atacaaataa tcattcatta
    29701 tttcttcttt gttaccacct tattttagta gtgaaacatt gtaaacatct cattgatgat
    29761 gtaatgttag cttatgcaaa aaactatctt tgaaagaata tcagaatcac atcacattac
    29821 cttagttgag tgaaatcgtg ataaagacta ctttcatatt atgctcaaag cacaacctaa
    29881 aaccgaatta acaaaattca gcaatgccta agctcatgtc tgataaaatg agactttcta
    29941 agggtcaaaa atttcctttg aatacgatca ttagcttaga taaaagtaaa aaccttagaa
    30001 ctacggggat agcttggtta ataagagata tcgctgttaa taaagacata agcttgcaag
    30061 tatgctctat tcccaagaat ctcccacttc aagcactaag gatcgttaaa ttcagctaag
    30121 tggcaagtag ttcactagac gataattatc tccatatttc aatctcagct tttccatttt
    30181 tcatagcaaa ttccgttatt tttatagcat ctaaaagatt cagcttttcc actgatcttt
    30241 caccactttt cagctttttt attgtgctta aattgatacc agtacctttt gatattgaat
    30301 tggaagtaaa atcattaagg gtttttttta ttaaattcat atccgcaatt ccagcgttca
    30361 taagaatgaa gattctcctc tcatagacta ctttgaaaat ggtactacag tggctgttta
    30421 tatatttcag taggttactg tagtggtcat tttattataa ttagaaaagg aacctaaatc
    30481 aaaaatgcta aagaaaattt tcatagtatt aaacctatca tgactaaagc cacggaattc
    30541 ttgataaaag agtatacttg caagcctatg tctttactaa cagcgatatc tcttattaac
    30601 caatctatcc ccgttgttcc taaggttctt gatgttttct aaggcaatgc ttgtatacat
    30661 agcccttgat caaaatattg atactaacgt tgacgtttcg atcatgatgt gtacggcaaa
    30721 taggacaagt ccattctcga attgtgaatg acttcttacc atctgtgtgt ccacattctg
    30781 aaccaatttg actagacgga aatcatttat ctatcttgat gatttttttg acagaagtat
    30841 cgaaaatctt tacataatct gggtgttgca ttcttatgca gatgagagcg ttagctccgt
    30901 attcataacc agttgcctct aaaacgtagt ccatcggtgg atacctagct tgcggttgcc
    30961 agtggctttc gcagtcttct tgtaatcaaa tcgagcatct aattgagtac tagtattttg
    31021 aagataatac ttgagttttt cggttttcag aagttcactt catcttccat catccatgga
    31081 aatcccactc acccatacgc aggcctccaa aaattcttca acaatggttt ttcagacatt
    31141 tttctgaata cctccttgtt ttttgatacg ccagatagtc aggaaaaagg caacaaaaca
    31201 caaaacaaag ctgcctaaaa aggtaatctt cattccatat aaaaagacat ccggccgatc
    31261 tgcgatatag ttagttactc gttggcccat cttagcagac atcgcttgat ataagaccgt
    31321 cgtagaaagc gaaatcccga tgaccatccc caaatttcgg gcaaaggaat tcatggagcc
    31381 tgccagtccc aaattttcga tagtgacaga actcataacc atcgtattat ttggggactg
    31441 gaacaaggca ttgccggcac ccatgatggc agtagcgaca atgtacagcc acaaaggaga
    31501 gtccactgac aaaaacatat acatgacttg ggttaccgac aataaaccca agcctactaa
    31561 tacaaaaatc tccgggccaa cccggtcggt caggtatccg cttaaaggcg ccccgacgac
    31621 catcatcaag ggaaagacca tcatcaacag tccggcatag ctaggactca acccgcgagc
    31681 attttgcaga taaaaaggaa tcactacatt aacaaagaag ttggaagaaa aaatcaacaa
    31741 ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa ataccgaaag aaatcaatgg
    31801 agaggctgct ttcttttcaa cttttaaaaa gctgacaaag gcaatcactg aaattacgaa
    31861 cagtacaagg gggactggat ggaaaaagcc actttcttgc cccaaaaaga ttgttccaaa
    31921 aaggctgata atcgtagccg caatggcagc aaagccccct ccatcgattg tatggccgct
    31981 catggtgata tcttttggta agaatttttc agcaatcaac atcgttacga ttcccaccgg
    32041 tacattgatc caaaaaatgt acgcccaaga aaattgggac aaaatcaagc cgccaagacc
    32101 tggtccagca atcgagccta atgaaacaag cgcaccgatt gctcccaatg cccgaccacg
    32161 ttcatgtatg ggaaagactt ccgtgatgat tccggcattt gtagccatag tcatactagc
    32221 accaatggct tgtaccaccc gtgcgaacaa ttaaatacct agcgaatgat tgaacccaca
    32281 caacagcgag cccaagacaa agatcagcgt accaatccga aatactttga ttttcccaca
    32341 gctatcgcca attttaccaa acagcaacaa acaagaacag acaatcatca gataaacgga
    32401 cactacccat tccgcttgat tcattggtat gcccatatca tctgagatgg taggtagtgc
    32461 aatattgaca atactgccat ccaatgtgga catgaaggta aacatagata tagcaatcaa
    32521 aatccaccag cgattttttt ggacttttgc atcttcttga taactatgca taataggatt
    32581 tcctttctaa tgatcgtgta gctaagaaca atataaccta tgtctactgc ccaacaactt
    32641 ctcgcgactc tagccatagg aagcttgagt aatttcgatt tgttattctc atgtaaccat
    32701 gtaagtttga gatgctttat ttgaacttac tctatagtaa ctagtgataa ttcgctttac
    32761 tttcttctag caatttctaa aagtcgactt cattgttctt cagattaata gtgtgagcta
    32821 tctcgtgaga cgtaagaaaa tttccattct actttttccc aattttttca tatttatggt
    32881 gttgcaccta tttgttttcc aatttgcttt tcaaatacat tgctatgata ttcaattcca
    32941 taggattata ctaaatgtca atagtatgaa taatatatta tagatgttta tatatcactg
    33001 ttaattatta tccacaataa ttaatgatta cctcgatatt tgtcaatcaa aggttgaaac
    33061 aatttatttt cagcatcttt tctaacttga gctgcactct taatagaatc atacgatcct
    33121 aaatagtacc tctttttttt aaagttatat acgcaatcca tttctcttta gaacgataaa
    33181 ctcccttaac accacttgta ttatttttcg gtaattttga cgttagacac tctattactg
    33241 tcccttcaac acatgtcctt gtttcttgcc tttttaaaca tccacaactt tgagtatgtc
    33301 caaaaaataa gttactataa cttatctcac acatactgcc acactcacat tgacaaaccc
    33361 attttgtttt ccatgaacag ttattgtcca aaggatactt tgcagtaagt aatccgaatt
    33421 ttttattttc aatgtgatgc ttttttgatt tcaaacatcc acagcttttt acatcacctt
    33481 ttagaagtcg ctttgccgat actaactttt ttctcccaca cagacattga cattcccaaa
    33541 caacagttcc ttcacttcgc agtttagttg gcttgattgc aactaactta ccaaaagttt
    33601 tatttgtcaa atttttatat ttatgcctat ttttataatt tccacatcca caacttttgg
    33661 ttctacctga tagtaagttt gatcgatata caatcgtttc attcccacag tcacataggt
    33721 aacgatattt tttatatcca ttatgagtaa tctcctcaac aacagtaagg tgaccatatc
    33781 tttccttcac tgcacctacc ctcccaagaa agaagtacca tataatttca attaactttt
    33841 aataaatcag aaaaaaatat tttcaatttt tcttagtctg attttatgac taatttataa
    33901 tacaagactg aacgatacgt acacgaaatc ctcaagatag ataaattgtt ttcatctagt
    33961 aaaacctgct caaaatatga gcataaagac ggcaataata cactcgatat tcgataatgg
    34021 acttgtttta tttgtcaaac tcatcgtaat cgagatatca attccagtct caatatcctg
    34081 atcaaaggtc taagaatcca accattttct tgttactttg tcagattgat tacctttttt
    34141 agtctccgaa taataaggtt ttcggatcaa ctaacatgct ctgagaaaga ctactatctt
    34201 tgcaaatttc caaatgaagg tgtgggccag tactattccc agtacttcct acatagccaa
    34261 ttatttgtcc ttgcaaaaca ttatctccag tttttactag gtattgttgc tggtgagcat
    34321 ataacgcggt tgatccatct tcgtgttgta tcaccacgta atttccccaa gagtagtgaa
    34381 actctgcttt aacaacctta ccagctttaa ctgcatgaat cggctcaccc tgtgtagctg
    34441 ccatatcaag tcctcggtga aattcatttc cacgatttcc aaacaaactg gttattgaat
    34501 aattatgaat aggcacttgg tatccagtct tactcatttg tggaccttta acttctttat
    34561 catagttggt taaatcatag gttttaatta attcatttag cttcaaatag tattgtgtat
    34621 ctgttgcata ccgtcctgta aggaatttag ttgcttctcg atatgtttta ctattagctt
    34681 ttaatgctcc tgcataaaaa tctttatttc cactaattcc cttagttaat aatttggcat
    34741 aatcttcaaa actttcctca tagttttcat acactctaaa ttttgattgg gtcgtataaa
    34801 gcgttccatt acccaaatct tcgtttgtta ccataatgac aaagtttcca ttataagttc
    34861 cttttatccc aaataaattg taattcggtg cctgagctaa ctggctttga ccactggcag
    34921 attctaagat cgcttgtgca atcataacag aagcatataa atcatatttc tgaccaattt
    34981 ttctcgcaga ctcaccaatt ttcataataa aattttctac agattcattt ttgtcaaaat
    35041 gaatttcttc attaccagat agtattggtt gtgattggaa agattctgtt gattctattt
    35101 gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt tggcgcttcc ttttttgaag
    35161 actctatatg gtttggtctt gtcactttcg aagaagttat ttcacttgta ctacttgaag
    35221 ttgttgaaga ttcggcactt gaaatcgtac tactttcact tgacgaagtt gttgaagact
    35281 cggcatttga agtcgtacta ctttcacttg atgaagttgt tgaagactca gcatttgaag
    35341 tcgtactact ttcacttgat aaagttgtca aaagttcact actctctgct gttacatcgg
    35401 aattactcat ttcagtcgtc tcggttcttt cttttgcaat tttgtatgtt gaattttcgc
    35461 ttgccaacac atggaaaggt gttgctataa agagttggga cattaatata actgctgatg
    35521 caagttttaa agattttttc atttgattta cctctctttt ttctctttaa tagtgttttc
    35581 tattaaaaat tatgataaat attttcaatg aattgagtaa tacagtatat ttcttgattt
    35641 catttagtaa cgattatgtt tcaatgttta atcctgttac tgcctttaaa ataaaattat
    35701 agggttggct aaaactagga tgaaataaat aatctaacat tttcaatttg tctaatgtaa
    35761 tttgttcttc tatagcaaga gaaaacattg ataatagtcc agataaatct tcttgactac
    35821 aaagttgtac acctactatt ctctttgtat ttttttcaaa gctaattctt aattttacac
    35881 gactattttt tctcaaaaaa acaggtctaa tgaaatcttc tatttctata taatctatag
    35941 actcacttaa taatcgtgct gtttttaaat tcaaacctgc tgcgtaaaaa ttgtaattaa
    36001 atatagaaaa gccatttgta aattgggaac cagaaggtgt aaaatcttga ttgtcaatat
    36061 gttgagttgc aatataagct gttcttagag cattagaaat agaaaaatca acactacttc
    36121 tattaatgga cgtaaaaaaa gttgtagcac aatccccaat agcatatata tcttttatat
    36181 ttgtttgctg gtattcatta gttagatatg ctccactaac atctctcaag agttcctttt
    36241 tgccaattgg actattagat agaaatccta tagcttgtac gaccatatcc atagagtatt
    36301 gggtaccact tgtagtgact attttttcaa tcttttttct tcctaaatat tttttaggac
    36361 tttctgatag acaaagatgt attccatttt ttgatagtat ttcttcaact ttatctgaaa
    36421 aactacggtc gtaataagtc gacaacactc tatctgcaac atcgaaaaga tatacttctt
    36481 tccctctttt tcttatggct tcagcaattt caactccaat atatcctgct ccaataattc
    36541 ctactttttt aatagtatta tcatctaaag ctttaaaaat ctctttagca tttggaaaat
    36601 tctttatcga aaaaatatta gctaaattat tccccggtac atctaacata atttgctttg
    36661 aacctgtagc tagtataagt ttgtcatatt cttgggaaaa tcttgtgcca tcttttttta
    36721 cacaaataat ctttttattt tgaatatcaa ttttgtcaac tgctgtctcc aaaaacaact
    36781 tttttatctt agaatttagt tctcttctat tttgataaaa aagatcttct aaattgctaa
    36841 cttctctttt tatgaacaac gatgtcccac attctaagta actaatttct gattttttat
    36901 ctatcagaat aacttcataa tttgaatcac ataagtttag aaatcgagct gcagctagcc
    36961 ccgaatggtt tgatccaata attactattt ttttcactta atcacattct tttcccttct
    37021 atgaaaagta tcttcatttt taactaacaa cctatctccc aaaataaaaa acaaaaagag
    37081 gaagaaaaag attcttcctc ttttcagaga agatttaaga aattgatttt ctattcattc
    37141 tctttttttc gatttttata ccctaataac cctaaagttc ctaacaggat taaaccacca
    37201 ctaatagtaa agatattact atcaatctct cccgtcttag gataagcttt tgaggactgc
    37261 ttttgaggac tgcttttgcc tctatttttg ttttgcttgc taccttgatt gtcagtctta
    37321 ttatctttac taacggcttt ttcactgtct ttttcgtttg aagaaacaac tacattagta
    37381 ggttcactag tattaccaga ttcatcagtt gcagttacta tcacttttgt tcctgcctct
    37441 aatttaggaa ctttaactgt aaaattacca tttttatcag ctgtcccatg gtattgactc
    37501 ccgtttggta atgtaactgt cacctctgca tttggttcag ttttaccagt aacaatttgg
    37561 tcaccctctg taataggatc aacttgaggt ttattcggtg cttttttatc ttctacagaa
    37621 atagtaacct ctacttcttc tttagatcca tctggatatg ttactactac aatccctttt
    37681 ttgtttccag ctgtactaac atctactttc tctttccaag tataagtagt tccttttggt
    37741 agatcttctt tgttcttaat tccctcagat gcgtctggct ctttatttaa gtctgtagtc
    37801 actttttgtc cttcaggatt gtatttatcg gcatcggaac gattgtctgt cacttctact
    37861 ggaactttca ctgtatcttt cgtaccatct ggatacgtta cttcgataac cccttcgtaa
    37921 ttacctggtg tgttggtatc aatcgtacca tcaggagtga catctgttac tgttgttcct
    37981 tctggtaatg ttggcaagtt agtaacatta tctgttagat ctactgtgcc tccaacttct
    38041 actttttcgc cttctactgt tggctcatat ttatcggcgt cagaacgatt gtctgtcact
    38101 gtaacatcta cttcaacttc atcagatgat ccatctggat atgtcactac aacagtccct
    38161 tttttatttc cagctgcgct aacatctact ttttctttcc aagtatactt agtatctttt
    38221 ggtaaatctt ctttgttctt aattccctca gatgcgtctg gttccttatt caattctgta
    38281 gtcacttttt gaccttcagg attatatttg tcagcgtcag at
//
LOCUS       DACECB010000020        37922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECB010000020 DACECB010000000
VERSION     DACECB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.020"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..1469)
                     /locus_tag="IWO04_001119"
     CDS             complement(159..1469)
                     /locus_tag="IWO04_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0212742.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IWO04_001120"
     CDS             complement(1481..2899)
                     /locus_tag="IWO04_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0212743.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IWO04_001121"
     CDS             complement(3139..4707)
                     /locus_tag="IWO04_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0212744.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IWO04_001122"
     CDS             complement(4711..5121)
                     /locus_tag="IWO04_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0212745.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IWO04_001123"
     CDS             complement(5118..6041)
                     /locus_tag="IWO04_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0212746.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IWO04_001124"
     CDS             complement(6038..6916)
                     /locus_tag="IWO04_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0212747.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IWO04_001125"
     CDS             complement(6903..8240)
                     /locus_tag="IWO04_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0212748.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IWO04_001126"
     CDS             complement(8243..9346)
                     /locus_tag="IWO04_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0212749.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IWO04_001127"
     CDS             complement(9420..10928)
                     /locus_tag="IWO04_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0212750.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IWO04_001128"
     CDS             complement(10894..11586)
                     /locus_tag="IWO04_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0212751.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IWO04_001129"
     CDS             complement(11608..12582)
                     /locus_tag="IWO04_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0212752.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IWO04_001130"
     CDS             12882..13754
                     /locus_tag="IWO04_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0212753.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IWO04_001131"
     CDS             complement(14212..16410)
                     /locus_tag="IWO04_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212754.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IWO04_001132"
     CDS             16642..17634
                     /locus_tag="IWO04_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0212755.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IWO04_001133"
     CDS             17694..19115
                     /locus_tag="IWO04_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0212756.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IWO04_001134"
     CDS             19108..20607
                     /locus_tag="IWO04_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0212757.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IWO04_001135"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IWO04_001135"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0212758.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IWO04_001136"
     CDS             22199..22882
                     /locus_tag="IWO04_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0212759.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLSSQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IWO04_001137"
     CDS             22884..23720
                     /locus_tag="IWO04_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0212760.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IWO04_001138"
     CDS             23731..25485
                     /locus_tag="IWO04_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212761.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IWO04_001139"
     CDS             complement(25761..26102)
                     /locus_tag="IWO04_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0212762.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IWO04_001140"
     CDS             complement(26170..28341)
                     /locus_tag="IWO04_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0212763.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IWO04_001141"
     CDS             28552..29409
                     /locus_tag="IWO04_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0212764.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IWO04_001142"
     CDS             complement(29480..30337)
                     /locus_tag="IWO04_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0212765.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IWO04_001143"
     CDS             complement(30322..33024)
                     /locus_tag="IWO04_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0212766.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IWO04_001144"
     CDS             complement(33037..34326)
                     /locus_tag="IWO04_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0212767.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IWO04_001145"
     CDS             34476..35522
                     /locus_tag="IWO04_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0212768.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IWO04_001146"
     CDS             35524..37677
                     /locus_tag="IWO04_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0212769.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11203 a         7808 c         6747 g        12164 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 atcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gt
//
LOCUS       DACECB010000021        37912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECB010000021 DACECB010000000
VERSION     DACECB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.021"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(322..1266)
                     /locus_tag="IWO04_001147"
     CDS             complement(322..1266)
                     /locus_tag="IWO04_001147"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0212770.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1562..2506)
                     /locus_tag="IWO04_001148"
     CDS             complement(1562..2506)
                     /locus_tag="IWO04_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212771.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2727..3941
                     /locus_tag="IWO04_001149"
     CDS             2727..3941
                     /locus_tag="IWO04_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0212772.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3963..5978
                     /locus_tag="IWO04_001150"
     CDS             3963..5978
                     /locus_tag="IWO04_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAQ0212773.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6061..7434)
                     /locus_tag="IWO04_001151"
     CDS             complement(6061..7434)
                     /locus_tag="IWO04_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0212774.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7460..7744)
                     /locus_tag="IWO04_001152"
     CDS             complement(7460..7744)
                     /locus_tag="IWO04_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0212775.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7746..9761)
                     /locus_tag="IWO04_001153"
     CDS             complement(7746..9761)
                     /locus_tag="IWO04_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0212776.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9940..10245)
                     /locus_tag="IWO04_001154"
     CDS             complement(9940..10245)
                     /locus_tag="IWO04_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0212777.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10334..11365)
                     /locus_tag="IWO04_001155"
     CDS             complement(10334..11365)
                     /locus_tag="IWO04_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0212778.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11572..12447
                     /locus_tag="IWO04_001156"
     CDS             11572..12447
                     /locus_tag="IWO04_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0212779.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12599..13441)
                     /locus_tag="IWO04_001157"
     CDS             complement(12599..13441)
                     /locus_tag="IWO04_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0212780.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13533..15410)
                     /locus_tag="IWO04_001158"
     CDS             complement(13533..15410)
                     /locus_tag="IWO04_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0212781.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15407..16828)
                     /locus_tag="IWO04_001159"
     CDS             complement(15407..16828)
                     /locus_tag="IWO04_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0212782.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16831..20220)
                     /locus_tag="IWO04_001160"
     CDS             complement(16831..20220)
                     /locus_tag="IWO04_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0212783.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20477..21868
                     /locus_tag="IWO04_001161"
     CDS             20477..21868
                     /locus_tag="IWO04_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0212784.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21941..22588)
                     /locus_tag="IWO04_001162"
     CDS             complement(21941..22588)
                     /locus_tag="IWO04_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0212785.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22596..23282)
                     /locus_tag="IWO04_001163"
     CDS             complement(22596..23282)
                     /locus_tag="IWO04_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212786.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23288..24361)
                     /locus_tag="IWO04_001164"
     CDS             complement(23288..24361)
                     /locus_tag="IWO04_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0212787.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24583..25257
                     /locus_tag="IWO04_001165"
     CDS             24583..25257
                     /locus_tag="IWO04_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212788.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25262..26602
                     /locus_tag="IWO04_001166"
     CDS             25262..26602
                     /locus_tag="IWO04_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0212789.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26751..27737)
                     /locus_tag="IWO04_001167"
     CDS             complement(26751..27737)
                     /locus_tag="IWO04_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0212790.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKT"
     gene            28461..29492
                     /locus_tag="IWO04_001168"
     CDS             28461..29492
                     /locus_tag="IWO04_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0212791.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29601..31133
                     /locus_tag="IWO04_001169"
     CDS             29601..31133
                     /locus_tag="IWO04_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0212792.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31272..32084
                     /locus_tag="IWO04_001170"
     CDS             31272..32084
                     /locus_tag="IWO04_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0212793.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32074..32559
                     /locus_tag="IWO04_001171"
     CDS             32074..32559
                     /locus_tag="IWO04_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0212794.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32582..33127
                     /locus_tag="IWO04_001172"
     CDS             32582..33127
                     /locus_tag="IWO04_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0212795.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33200..34843)
                     /locus_tag="IWO04_001173"
     CDS             complement(33200..34843)
                     /locus_tag="IWO04_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0212796.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34972..37200)
                     /locus_tag="IWO04_001174"
     CDS             complement(34972..37200)
                     /locus_tag="IWO04_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0212797.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37233..37712)
                     /locus_tag="IWO04_001175"
     CDS             complement(37233..37712)
                     /locus_tag="IWO04_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212798.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11054 a         7179 c         6900 g        12779 t
ORIGIN      
        1 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
       61 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
      121 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
      181 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta
      241 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
      301 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
      361 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      421 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      481 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      541 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      601 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      661 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      721 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      781 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      841 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      901 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      961 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
     1021 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
     1081 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
     1141 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
     1201 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
     1261 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1321 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1381 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1441 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1501 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1561 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1621 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1681 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1741 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1801 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1861 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1921 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1981 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     2041 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     2101 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     2161 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     2221 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     2281 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2341 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2401 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2461 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
     2521 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
     2581 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
     2641 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
     2701 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
     2761 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
     2821 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
     2881 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
     2941 aacaaactgt gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct
     3001 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
     3061 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
     3121 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
     3181 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
     3241 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg
     3301 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
     3361 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
     3421 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
     3481 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
     3541 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
     3601 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
     3661 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
     3721 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3781 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3841 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3901 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3961 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     4021 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     4081 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     4141 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     4201 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     4261 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4321 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4381 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4441 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4501 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4561 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4621 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4681 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4741 cttctttact gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt
     4801 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4861 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4921 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4981 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     5041 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     5101 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
     5161 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
     5221 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
     5281 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
     5341 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
     5401 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
     5461 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
     5521 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
     5581 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
     5641 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
     5701 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
     5761 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
     5821 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
     5881 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
     5941 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
     6001 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
     6061 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
     6121 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
     6181 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
     6241 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
     6301 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
     6361 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
     6421 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
     6481 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
     6541 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
     6601 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
     6661 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
     6721 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
     6781 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
     6841 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
     6901 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
     6961 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
     7021 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
     7081 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
     7141 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
     7201 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
     7261 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
     7321 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
     7381 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
     7441 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
     7501 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
     7561 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
     7621 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
     7681 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
     7741 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
     7801 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
     7861 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
     7921 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
     7981 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
     8041 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
     8101 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
     8161 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
     8221 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
     8281 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
     8341 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
     8401 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
     8461 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
     8521 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
     8581 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
     8641 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
     8701 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
     8761 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
     8821 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
     8881 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
     8941 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
     9001 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
     9061 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
     9121 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
     9181 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
     9241 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
     9301 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
     9361 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
     9421 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
     9481 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
     9541 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
     9601 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
     9661 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
     9721 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
     9781 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
     9841 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
     9901 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
     9961 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    10021 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    10081 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    10141 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    10201 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    10261 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    10321 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    10381 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    10441 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    10501 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    10561 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    10621 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    10681 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    10741 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    10801 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    10861 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    10921 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    10981 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    11041 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    11101 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    11161 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    11221 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    11281 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    11341 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    11401 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    11461 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    11521 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    11581 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    11641 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    11701 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    11761 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    11821 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    11881 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    11941 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    12001 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    12061 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    12121 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    12181 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    12241 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    12301 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    12361 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    12421 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    12481 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    12541 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    12601 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    12661 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    12721 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    12781 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    12841 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    12901 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    12961 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    13021 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    13081 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    13141 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    13201 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    13261 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    13321 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    13381 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    13441 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    13501 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    13561 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    13621 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    13681 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    13741 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    13801 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    13861 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    13921 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    13981 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    14041 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    14101 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    14161 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    14221 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    14281 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    14341 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    14401 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    14461 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    14521 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    14581 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    14641 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    14701 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    14761 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    14821 tttgtaactt tcaacgttcc atcattacag actgtatttt tagggtagag atggatagta
    14881 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    14941 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    15001 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    15061 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    15121 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    15181 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    15241 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    15301 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    15361 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    15421 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    15481 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    15541 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    15601 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    15661 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    15721 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    15781 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    15841 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    15901 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    15961 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    16021 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    16081 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    16141 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    16201 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    16261 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    16321 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    16381 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    16441 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    16501 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    16561 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    16621 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    16681 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    16741 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    16801 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    16861 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    16921 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    16981 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    17041 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    17101 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    17161 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    17221 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    17281 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    17341 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    17401 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    17461 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    17521 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    17581 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    17641 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    17701 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    17761 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    17821 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    17881 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    17941 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    18001 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    18061 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    18121 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    18181 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    18241 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    18301 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    18361 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    18421 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    18481 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    18541 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    18601 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    18661 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    18721 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    18781 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    18841 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    18901 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    18961 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    19021 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    19081 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    19141 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    19201 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    19261 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    19321 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    19381 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    19441 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    19501 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    19561 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    19621 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    19681 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    19741 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    19801 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    19861 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    19921 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    19981 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    20041 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    20101 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    20161 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    20221 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    20281 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    20341 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    20401 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    20461 atgcaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    20521 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    20581 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    20641 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    20701 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    20761 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    20821 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    20881 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    20941 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    21001 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    21061 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    21121 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    21181 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    21241 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    21301 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    21361 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    21421 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    21481 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    21541 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    21601 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    21661 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    21721 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    21781 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    21841 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    21901 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    21961 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    22021 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    22081 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    22141 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    22201 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    22261 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    22321 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    22381 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    22441 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    22501 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    22561 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    22621 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    22681 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    22741 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    22801 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    22861 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    22921 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    22981 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    23041 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    23101 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    23161 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    23221 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    23281 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    23341 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    23401 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    23461 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    23521 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    23581 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    23641 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    23701 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    23761 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    23821 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    23881 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    23941 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    24001 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    24061 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    24121 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    24181 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    24241 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    24301 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    24361 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    24421 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    24481 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    24541 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    24601 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    24661 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    24721 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    24781 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    24841 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    24901 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    24961 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    25021 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    25081 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    25141 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    25201 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    25261 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    25321 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    25381 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    25441 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    25501 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    25561 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    25621 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    25681 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    25741 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    25801 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    25861 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    25921 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    25981 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    26041 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    26101 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    26161 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    26221 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    26281 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    26341 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    26401 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    26461 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    26521 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    26581 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    26641 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    26701 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgttttt
    26761 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    26821 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    26881 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    26941 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    27001 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    27061 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    27121 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    27181 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    27241 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    27301 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    27361 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    27421 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    27481 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    27541 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    27601 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    27661 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    27721 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    27781 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    27841 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    27901 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg
    27961 tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat
    28021 tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac
    28081 tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa
    28141 taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa
    28201 gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag
    28261 ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta
    28321 aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta
    28381 agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac
    28441 actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa
    28501 agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt
    28561 ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt
    28621 tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag
    28681 attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga
    28741 accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt
    28801 cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc
    28861 ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac
    28921 ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag
    28981 ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt
    29041 gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc
    29101 agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag
    29161 cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt
    29221 ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga
    29281 gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt
    29341 atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc
    29401 tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat
    29461 tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat
    29521 aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa
    29581 caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta
    29641 cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac
    29701 tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt
    29761 tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt
    29821 ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag
    29881 tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt
    29941 tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa
    30001 atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg
    30061 tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa
    30121 ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc
    30181 aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag
    30241 tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc
    30301 ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg
    30361 cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag
    30421 aagctttttc gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa
    30481 tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc
    30541 aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg
    30601 atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag
    30661 ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg
    30721 ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg
    30781 atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag
    30841 tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt
    30901 tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta
    30961 ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac
    31021 ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa
    31081 tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta
    31141 aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat
    31201 aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg
    31261 aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag
    31321 gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta agcgccttga
    31381 cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat
    31441 tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg
    31501 ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa
    31561 atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc
    31621 aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta
    31681 atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga
    31741 tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca
    31801 caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat
    31861 tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg
    31921 caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa
    31981 aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct
    32041 ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat
    32101 gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct
    32161 acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt
    32221 ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga
    32281 tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt
    32341 tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt
    32401 atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc tggagtggct
    32461 tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact
    32521 gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa
    32581 aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt
    32641 ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt
    32701 cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac
    32761 gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt
    32821 gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc
    32881 tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa
    32941 tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc
    33001 aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg
    33061 acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa
    33121 ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat
    33181 gaactttaac gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta
    33241 gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact
    33301 gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa
    33361 gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat
    33421 attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct
    33481 tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg
    33541 tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag
    33601 tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt
    33661 cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat
    33721 caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa
    33781 taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc
    33841 aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca
    33901 tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt
    33961 tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa
    34021 attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag
    34081 tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat
    34141 cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg
    34201 cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta
    34261 tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt
    34321 cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat
    34381 gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat
    34441 ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt
    34501 cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga
    34561 tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct
    34621 cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga
    34681 tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga
    34741 tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt
    34801 gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta
    34861 acggtccctc ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag
    34921 aaacgaaaag ctcaacgaat ctgctaagtg cgttcgttga gctttatttt atcatagata
    34981 gcgggcaatc agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa
    35041 acgactttca ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac
    35101 aaataggatt ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt
    35161 tttgcctgta taatccggca atgcttttgg ctgaccgtca ctaatattaa gcaacagttt
    35221 ggttgttgcg gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg
    35281 attgttttgc cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc
    35341 aaattcttta taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc
    35401 gtaaatcaac aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt
    35461 ttttgccgct tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc
    35521 taccgctaaa gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc
    35581 atatgctact ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc
    35641 tgtttgttca ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat
    35701 tacttgcatg tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac
    35761 gatgagacct tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag
    35821 ttgcttcttg atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc
    35881 atctcctgtt tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg
    35941 catttgagga ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt
    36001 tggttcttca gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc
    36061 agtgatgttt cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga
    36121 ttgaggatga ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact
    36181 gtcagacaaa gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa
    36241 aacttttgga taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata
    36301 aaaataagaa cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa
    36361 caaacggata atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc
    36421 catcgcacgt tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat
    36481 ccgatctctt aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat
    36541 cacaatgacg caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt
    36601 tggttcgagc gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc
    36661 ttgaatctct aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct
    36721 gtaatggatt gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc
    36781 tttagcagaa aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag
    36841 tgtatctgtt tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac
    36901 tacaggtaat aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt
    36961 tgtataggga agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc
    37021 ggcatctcct tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt
    37081 gatttcggag gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca
    37141 gatcatttgc ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat
    37201 gcttctcttc cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt
    37261 tttaaaatac ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac
    37321 aaaatgatgt cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc
    37381 ccttgtaatc caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga
    37441 taagcatctt ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt
    37501 gtgattggta attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg
    37561 cctgagatat cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt
    37621 gcatccatat ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact
    37681 ggtttatgtt ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt
    37741 agtaaataag taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga
    37801 ttgaagtgag gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt
    37861 tcttttacac gctcattagt aacatgtaga aagtccaaga tgatgttgga at
//
LOCUS       DACECB010000022        37103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECB010000022 DACECB010000000
VERSION     DACECB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.022"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(161..445)
                     /locus_tag="IWO04_001176"
     CDS             complement(161..445)
                     /locus_tag="IWO04_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212799.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IWO04_001177"
     CDS             complement(463..1353)
                     /locus_tag="IWO04_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0212800.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IWO04_001178"
     CDS             complement(1373..1591)
                     /locus_tag="IWO04_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212801.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IWO04_001179"
     CDS             complement(1561..3261)
                     /locus_tag="IWO04_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0212802.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IWO04_001180"
     CDS             3528..4301
                     /locus_tag="IWO04_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212803.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IWO04_001181"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IWO04_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0212804.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IWO04_001182"
     CDS             complement(5125..6096)
                     /locus_tag="IWO04_001182"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0212805.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IWO04_001183"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IWO04_001183"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0212806.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IWO04_001184"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IWO04_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0212807.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IWO04_001185"
     CDS             complement(9051..9875)
                     /locus_tag="IWO04_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0212808.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IWO04_001186"
     CDS             complement(9848..10528)
                     /locus_tag="IWO04_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212809.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IWO04_001187"
     CDS             complement(10551..11489)
                     /locus_tag="IWO04_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0212810.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IWO04_001188"
     CDS             complement(11734..12585)
                     /locus_tag="IWO04_001188"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0212811.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IWO04_001189"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IWO04_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0212812.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IWO04_001190"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IWO04_001190"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0212813.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IWO04_001191"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IWO04_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0212814.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IWO04_001192"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IWO04_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0212815.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IWO04_001193"
     CDS             complement(19463..20704)
                     /locus_tag="IWO04_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0212816.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IWO04_001194"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IWO04_001194"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0212817.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IWO04_001195"
     CDS             complement(21731..22615)
                     /locus_tag="IWO04_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212818.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="IWO04_001196"
     CDS             complement(23393..24679)
                     /locus_tag="IWO04_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0212819.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="IWO04_001197"
     CDS             complement(24672..25940)
                     /locus_tag="IWO04_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0212820.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="IWO04_001198"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="IWO04_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0212821.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="IWO04_001199"
     CDS             complement(26712..27158)
                     /locus_tag="IWO04_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212822.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27251..27496)
                     /locus_tag="IWO04_001200"
     CDS             complement(27251..27496)
                     /locus_tag="IWO04_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212823.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27611..28189)
                     /locus_tag="IWO04_001201"
     CDS             complement(27611..28189)
                     /locus_tag="IWO04_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0212824.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28266..28796)
                     /locus_tag="IWO04_001202"
     CDS             complement(28266..28796)
                     /locus_tag="IWO04_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0212825.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IWO04_001203"
     CDS             complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IWO04_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0212826.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29653..30048)
                     /locus_tag="IWO04_001204"
     CDS             complement(29653..30048)
                     /locus_tag="IWO04_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0212827.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30045..31457)
                     /locus_tag="IWO04_001205"
     CDS             complement(30045..31457)
                     /locus_tag="IWO04_001205"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0212828.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IWO04_001206"
     CDS             complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IWO04_001206"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0212829.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32312..33772)
                     /locus_tag="IWO04_001207"
     CDS             complement(32312..33772)
                     /locus_tag="IWO04_001207"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0212830.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33942..35099)
                     /locus_tag="IWO04_001208"
     CDS             complement(33942..35099)
                     /locus_tag="IWO04_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0212831.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IWO04_001209"
     CDS             complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IWO04_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0212832.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36566..37093)
                     /locus_tag="IWO04_001210"
     CDS             complement(36566..37093)
                     /locus_tag="IWO04_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0212833.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10249 a         8086 c         6507 g        12261 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    27301 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    27361 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    27421 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    27481 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    27541 ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
    27601 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
    27661 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
    27721 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
    27781 cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg
    27841 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
    27901 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
    27961 ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
    28021 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
    28081 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
    28141 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
    28201 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
    28261 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
    28321 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
    28381 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
    28441 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
    28501 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
    28561 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
    28621 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
    28681 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
    28741 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
    28801 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
    28861 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
    28921 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
    28981 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
    29041 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
    29101 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
    29161 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
    29221 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
    29281 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
    29341 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
    29401 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
    29461 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
    29521 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
    29581 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
    29641 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
    29701 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
    29761 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
    29821 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
    29881 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
    29941 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
    30001 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
    30061 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
    30121 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
    30181 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
    30241 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
    30301 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
    30361 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
    30421 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
    30481 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
    30541 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
    30601 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
    30661 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
    30721 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
    30781 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
    30841 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
    30901 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
    30961 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
    31021 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
    31081 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggc
    31141 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
    31201 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
    31261 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
    31321 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
    31381 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
    31441 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
    31501 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
    31561 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
    31621 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
    31681 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
    31741 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
    31801 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
    31861 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
    31921 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
    31981 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
    32041 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
    32101 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
    32161 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
    32221 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
    32281 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
    32341 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
    32401 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
    32461 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
    32521 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
    32581 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
    32641 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
    32701 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
    32761 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
    32821 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
    32881 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
    32941 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
    33001 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
    33061 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
    33121 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
    33181 aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg atattatcgg
    33241 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
    33301 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
    33361 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
    33421 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
    33481 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
    33541 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
    33601 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
    33661 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
    33721 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
    33781 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
    33841 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
    33901 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
    33961 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
    34021 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
    34081 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
    34141 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
    34201 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
    34261 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
    34321 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
    34381 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
    34441 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
    34501 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
    34561 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
    34621 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
    34681 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
    34741 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
    34801 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
    34861 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
    34921 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
    34981 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
    35041 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
    35101 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
    35161 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
    35221 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
    35281 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
    35341 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
    35401 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
    35461 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
    35521 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
    35581 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
    35641 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
    35701 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
    35761 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
    35821 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
    35881 ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg gctgacactt
    35941 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
    36001 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
    36061 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
    36121 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
    36181 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
    36241 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
    36301 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
    36361 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
    36421 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
    36481 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
    36541 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
    36601 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
    36661 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
    36721 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
    36781 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
    36841 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
    36901 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
    36961 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
    37021 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
    37081 ctctttttct caccggtttt cct
//
LOCUS       DACECB010000023        35319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECB010000023 DACECB010000000
VERSION     DACECB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.023"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IWO04_001211"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IWO04_001211"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0212834.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IWO04_001212"
     CDS             1011..2258
                     /locus_tag="IWO04_001212"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0212835.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IWO04_001213"
     CDS             2334..2678
                     /locus_tag="IWO04_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212836.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IWO04_001214"
     CDS             complement(2795..3121)
                     /locus_tag="IWO04_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212837.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="IWO04_001215"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="IWO04_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="IWO04_001216"
     CDS             4812..5327
                     /locus_tag="IWO04_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0212838.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="IWO04_001217"
     CDS             complement(5526..5972)
                     /locus_tag="IWO04_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0212839.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IWO04_001218"
     CDS             complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IWO04_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0212840.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="IWO04_001219"
     CDS             complement(7851..8480)
                     /locus_tag="IWO04_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0212841.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="IWO04_001220"
     CDS             complement(8464..8928)
                     /locus_tag="IWO04_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0212842.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="IWO04_001221"
     CDS             complement(9415..10683)
                     /locus_tag="IWO04_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0212843.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="IWO04_001222"
     CDS             complement(10914..11651)
                     /locus_tag="IWO04_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0212844.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="IWO04_001223"
     CDS             complement(11718..12287)
                     /locus_tag="IWO04_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0212845.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="IWO04_001224"
     CDS             12415..13287
                     /locus_tag="IWO04_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0212846.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="IWO04_001225"
     CDS             13629..14501
                     /locus_tag="IWO04_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0212847.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="IWO04_001226"
     CDS             14498..16456
                     /locus_tag="IWO04_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0212848.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="IWO04_001227"
     CDS             complement(16707..17558)
                     /locus_tag="IWO04_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0212849.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="IWO04_001228"
     CDS             17681..18148
                     /locus_tag="IWO04_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0212850.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="IWO04_001229"
     CDS             complement(18411..18893)
                     /locus_tag="IWO04_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0212851.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="IWO04_001230"
     CDS             complement(18993..19856)
                     /locus_tag="IWO04_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0212852.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="IWO04_001231"
     CDS             20454..21302
                     /locus_tag="IWO04_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212853.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="IWO04_001232"
     CDS             complement(21473..21814)
                     /locus_tag="IWO04_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0212854.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="IWO04_001233"
     CDS             21900..22607
                     /locus_tag="IWO04_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0212855.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="IWO04_001234"
     CDS             22594..22917
                     /locus_tag="IWO04_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0212856.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="IWO04_001235"
     CDS             complement(23006..24043)
                     /locus_tag="IWO04_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0212857.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="IWO04_001236"
     CDS             complement(24490..25473)
                     /locus_tag="IWO04_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0212858.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="IWO04_001237"
     CDS             complement(25889..26659)
                     /locus_tag="IWO04_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212859.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="IWO04_001238"
     CDS             complement(26652..27587)
                     /locus_tag="IWO04_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212860.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="IWO04_001239"
     CDS             complement(28181..29830)
                     /locus_tag="IWO04_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0212861.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="IWO04_001240"
     CDS             complement(30025..30921)
                     /locus_tag="IWO04_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0212862.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="IWO04_001241"
     CDS             complement(30914..31594)
                     /locus_tag="IWO04_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212863.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="IWO04_001242"
     CDS             complement(31595..32377)
                     /locus_tag="IWO04_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0212864.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="IWO04_001243"
     CDS             complement(32370..33287)
                     /locus_tag="IWO04_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212865.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="IWO04_001244"
     CDS             complement(33290..33574)
                     /locus_tag="IWO04_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0212866.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34275..34895)
                     /locus_tag="IWO04_001245"
     CDS             complement(34275..34895)
                     /locus_tag="IWO04_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212867.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(34976..>35319)
                     /locus_tag="IWO04_001246"
     CDS             complement(34976..>35319)
                     /locus_tag="IWO04_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212868.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        10574 a         7352 c         6244 g        11149 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttaaacaaa actctatttt attgcatttt acaggaatgt atttcatgcg aaattaataa
    34141 gtctttgttt atagagtttt actagggtat aaaaagttgt atgttatcat tacctcaaaa
    34201 cattacctca gcttggcgag ttggttgatt gaagaataaa tcatttatct agatatctca
    34261 ataaaaaatg atttctaaga aacttgtatt ttcgtgttat tatttttttg ccctatcaaa
    34321 atttctaatt cttctagcgt aatatgatta tgacacataa tcgattcaat tacttcaata
    34381 cctatttttt tcgagaaaga tcccttttgt tcactcacaa aaatactttc agaagacata
    34441 tataaggaac gaagttttgg aattagtata tcgtacaaat agaccaccat aaaatcgtcc
    34501 ttgcaaccat tctcaaaaag aattaaattt gctgattgtg ttacatatat cactgaatta
    34561 cattcatggt caattccaaa aacaatgtct acttcatttt cttttctaat tcgcgcagtt
    34621 acatttttca aactttcacc attacttata aatgctttag ttactgtaag ctctgatgtt
    34681 tgttcagata ttccttttat aatattttgt tcatcagcca cattccccat aaatccgccg
    34741 attcctttta agtaataatt ctcacctaag agtcgcttgc attcactgaa ttgataaaaa
    34801 aaccagttaa aaaaatcttc tggaaaaata aattctgatc gaactttatt tatattttta
    34861 gctcctatta attttataac tcgattaatg ttcacttgct gcgatgaaca tataactgaa
    34921 taaattgagt tgtttaattc aaaaaaacaa ctttaacata tgcatcatta attcgttatt
    34981 ttttaggtag atattgatca ttgttatctc tctttttttg cttcccatac tccaacttag
    35041 aaattgccgt tataaaattt attttaaagg tgactttttc ccctgaagta tcggtagtag
    35101 gaatacttaa tacctcagct tctgaggagg tatcccaatt atagttatac tttgcatcat
    35161 caccataaag aacaattcca ttttttttat cttgtgccgt tagctttact ggttcgctta
    35221 cttttcttgt taatatcttg gactttacat catcgatatt gcttatttta tcaagcttaa
    35281 ataatttaca aattaatttc gtactagttt ttatatttg
//
LOCUS       DACECB010000024        34603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECB010000024 DACECB010000000
VERSION     DACECB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.024"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO04_001247"
     CDS             591..1280
                     /locus_tag="IWO04_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212869.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO04_001248"
     CDS             1267..2475
                     /locus_tag="IWO04_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0212870.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO04_001249"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO04_001249"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0212871.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO04_001250"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO04_001250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0212872.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWO04_001251"
     CDS             complement(6578..7273)
                     /locus_tag="IWO04_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0212873.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO04_001252"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO04_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0212874.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWO04_001253"
     CDS             complement(8472..8663)
                     /locus_tag="IWO04_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0212875.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWO04_001254"
     CDS             complement(8684..9574)
                     /locus_tag="IWO04_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0212876.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWO04_001255"
     CDS             complement(9561..10328)
                     /locus_tag="IWO04_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0212877.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO04_001256"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO04_001256"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0212878.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO04_001257"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO04_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0212879.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO04_001258"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO04_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0212880.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWO04_001259"
     CDS             14944..15282
                     /locus_tag="IWO04_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212881.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWO04_001260"
     CDS             complement(15450..16208)
                     /locus_tag="IWO04_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0212882.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWO04_001261"
     CDS             complement(16226..17044)
                     /locus_tag="IWO04_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0212883.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO04_001262"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO04_001262"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0212884.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO04_001263"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO04_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0212885.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO04_001264"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO04_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0212886.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO04_001265"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO04_001265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0212887.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO04_001266"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO04_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0212888.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWO04_001267"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWO04_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0212889.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO04_001268"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO04_001268"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0212890.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO04_001269"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO04_001269"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0212891.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWO04_001270"
     CDS             27086..27385
                     /locus_tag="IWO04_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0212892.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWO04_001271"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWO04_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0212893.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO04_001272"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO04_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0212894.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30316
                     /locus_tag="IWO04_001273"
     CDS             28352..30316
                     /locus_tag="IWO04_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ0212895.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTARTF
                     DVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEYNSV
                     IAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFTNAA
                     TQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30322..30774
                     /gene="rplI"
                     /locus_tag="IWO04_001274"
     CDS             30322..30774
                     /gene="rplI"
                     /locus_tag="IWO04_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0212896.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31048..32415
                     /gene="dnaB"
                     /locus_tag="IWO04_001275"
     CDS             31048..32415
                     /gene="dnaB"
                     /locus_tag="IWO04_001275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0212897.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33195..34487
                     /locus_tag="IWO04_001276"
     CDS             33195..34487
                     /locus_tag="IWO04_001276"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0212898.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10549 a         6722 c         6839 g        10493 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttca gagctagttt ctgagctgat
    29761 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29821 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29881 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29941 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    30001 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    30061 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    30121 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    30181 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30241 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30301 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30361 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30421 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30481 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30541 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30601 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30661 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30721 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30781 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30841 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30901 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30961 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    31021 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    31081 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    31141 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    31201 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31261 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31321 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31381 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31441 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31501 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31561 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31621 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31681 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31741 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31801 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31861 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31921 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31981 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    32041 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    32101 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    32161 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    32221 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32281 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32341 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32401 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32461 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32521 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32581 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32641 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32701 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32761 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32821 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32881 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32941 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    33001 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    33061 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    33121 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    33181 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33241 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33301 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33361 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33421 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33481 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33541 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33601 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33661 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33721 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33781 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33841 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33901 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33961 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    34021 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    34081 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    34141 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    34201 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34261 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34321 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34381 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34441 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34501 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34561 acttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DACECB010000025        33919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECB010000025 DACECB010000000
VERSION     DACECB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.025"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            401..2062
                     /locus_tag="IWO04_001277"
     CDS             401..2062
                     /locus_tag="IWO04_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0212899.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2132..2479)
                     /locus_tag="IWO04_001278"
     CDS             complement(2132..2479)
                     /locus_tag="IWO04_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0212900.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2563..3504
                     /locus_tag="IWO04_001279"
     CDS             2563..3504
                     /locus_tag="IWO04_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212901.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3505..4548
                     /locus_tag="IWO04_001280"
     CDS             3505..4548
                     /locus_tag="IWO04_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0212902.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4554..5624
                     /locus_tag="IWO04_001281"
     CDS             4554..5624
                     /locus_tag="IWO04_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212903.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5614..6567
                     /locus_tag="IWO04_001282"
     CDS             5614..6567
                     /locus_tag="IWO04_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212904.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6579..7589
                     /locus_tag="IWO04_001283"
     CDS             6579..7589
                     /locus_tag="IWO04_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0212905.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7874..8695)
                     /locus_tag="IWO04_001284"
     CDS             complement(7874..8695)
                     /locus_tag="IWO04_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0212906.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8792..9070)
                     /locus_tag="IWO04_001285"
     CDS             complement(8792..9070)
                     /locus_tag="IWO04_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212907.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9338..11347
                     /gene="metG"
                     /locus_tag="IWO04_001286"
     CDS             9338..11347
                     /gene="metG"
                     /locus_tag="IWO04_001286"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0212908.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11826..12413)
                     /locus_tag="IWO04_001287"
     CDS             complement(11826..12413)
                     /locus_tag="IWO04_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212909.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12588..13910
                     /locus_tag="IWO04_001288"
     CDS             12588..13910
                     /locus_tag="IWO04_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0212910.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14065..14841
                     /locus_tag="IWO04_001289"
     CDS             14065..14841
                     /locus_tag="IWO04_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0212911.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14834..15403
                     /gene="rnmV"
                     /locus_tag="IWO04_001290"
     CDS             14834..15403
                     /gene="rnmV"
                     /locus_tag="IWO04_001290"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0212912.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15396..16280
                     /gene="rsmA"
                     /locus_tag="IWO04_001291"
     CDS             15396..16280
                     /gene="rsmA"
                     /locus_tag="IWO04_001291"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0212913.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16731..17360
                     /locus_tag="IWO04_001292"
     CDS             16731..17360
                     /locus_tag="IWO04_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212914.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17484..18593)
                     /gene="ugpC"
                     /locus_tag="IWO04_001293"
     CDS             complement(17484..18593)
                     /gene="ugpC"
                     /locus_tag="IWO04_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0212915.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18802..19224
                     /gene="mgsA"
                     /locus_tag="IWO04_001294"
     CDS             18802..19224
                     /gene="mgsA"
                     /locus_tag="IWO04_001294"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0212916.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19547..20083
                     /locus_tag="IWO04_001295"
     CDS             19547..20083
                     /locus_tag="IWO04_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0212917.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20123..21886)
                     /locus_tag="IWO04_001296"
     CDS             complement(20123..21886)
                     /locus_tag="IWO04_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212918.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21889..23604)
                     /locus_tag="IWO04_001297"
     CDS             complement(21889..23604)
                     /locus_tag="IWO04_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212919.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24383..24607)
                     /locus_tag="IWO04_001298"
     CDS             complement(24383..24607)
                     /locus_tag="IWO04_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212920.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24807..27575
                     /locus_tag="IWO04_001299"
     CDS             24807..27575
                     /locus_tag="IWO04_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0212921.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27656..28075
                     /locus_tag="IWO04_001300"
     CDS             27656..28075
                     /locus_tag="IWO04_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0212922.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28087..28557
                     /locus_tag="IWO04_001301"
     CDS             28087..28557
                     /locus_tag="IWO04_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0212923.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28574..29353
                     /locus_tag="IWO04_001302"
     CDS             28574..29353
                     /locus_tag="IWO04_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0212924.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29340..30161
                     /locus_tag="IWO04_001303"
     CDS             29340..30161
                     /locus_tag="IWO04_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0212925.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30178..31227
                     /locus_tag="IWO04_001304"
     CDS             30178..31227
                     /locus_tag="IWO04_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0212926.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31240..32244
                     /locus_tag="IWO04_001305"
     CDS             31240..32244
                     /locus_tag="IWO04_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0212927.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32877..33896
                     /locus_tag="IWO04_001306"
     CDS             32877..33896
                     /locus_tag="IWO04_001306"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0212928.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11072 a         6005 c         6928 g         9914 t
ORIGIN      
        1 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa
       61 tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa gggactgtga
      121 caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat ggcaataata
      181 gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc ttgtacaaag
      241 ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt atgaaaaaga
      301 caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa cttcagaaaa
      361 tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt
      421 tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac
      481 aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt
      541 gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc
      601 attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc
      661 aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg
      721 agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg
      781 gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga
      841 aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt
      901 tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct
      961 tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta
     1021 tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg
     1081 accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac
     1141 agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt
     1201 attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc
     1261 aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca
     1321 acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat
     1381 cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt
     1441 gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg
     1501 aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga
     1561 attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa
     1621 agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc
     1681 aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt
     1741 actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac
     1801 aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc
     1861 agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat
     1921 caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct
     1981 agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa
     2041 atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg
     2101 gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa
     2161 tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc
     2221 tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat
     2281 ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga
     2341 taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg
     2401 aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg
     2461 ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta
     2521 gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa
     2581 tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc
     2641 tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct
     2701 gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat
     2761 gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac
     2821 cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag
     2881 gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa
     2941 aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac
     3001 tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc
     3061 gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg
     3121 cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat
     3181 gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt
     3241 cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc
     3301 ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag
     3361 tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg
     3421 ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt
     3481 gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca
     3541 gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag
     3601 attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa
     3661 gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc
     3721 tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt
     3781 attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt
     3841 gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt
     3901 ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca
     3961 tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg
     4021 ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca
     4081 aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg
     4141 gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg
     4201 aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa
     4261 attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt
     4321 agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg
     4381 cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg
     4441 ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta
     4501 cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga
     4561 aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag
     4621 cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat
     4681 ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca
     4741 atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag
     4801 agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt
     4861 tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag
     4921 tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg
     4981 aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga
     5041 tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa
     5101 cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa
     5161 aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg
     5221 atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt
     5281 tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata
     5341 gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa
     5401 aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac
     5461 caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg
     5521 cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac
     5581 aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa
     5641 ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc
     5701 agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa
     5761 tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc
     5821 gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat
     5881 gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat
     5941 atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa
     6001 aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct
     6061 catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa
     6121 ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa
     6181 gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca
     6241 catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg
     6301 ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa
     6361 agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc
     6421 aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa
     6481 cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa
     6541 aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc
     6601 gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat
     6661 ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca
     6721 atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca
     6781 gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg
     6841 gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca
     6901 acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag
     6961 aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt
     7021 ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa
     7081 aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat
     7141 ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg
     7201 cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc
     7261 aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga
     7321 accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact
     7381 tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc
     7441 ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga
     7501 tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa
     7561 tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg
     7621 ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga
     7681 aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt
     7741 ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa
     7801 aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct
     7861 tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc
     7921 cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat
     7981 tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt
     8041 ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat
     8101 tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt
     8161 tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat
     8221 ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg
     8281 cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg
     8341 gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca
     8401 caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga
     8461 caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca
     8521 aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag
     8581 agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac
     8641 ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac
     8701 cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc
     8761 aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga
     8821 gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg
     8881 caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg
     8941 tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat
     9001 agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt
     9061 tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt
     9121 gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt
     9181 attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag
     9241 tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa
     9301 acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta
     9361 tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac
     9421 gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct
     9481 cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa
     9541 accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga
     9601 tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa
     9661 aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta
     9721 ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga
     9781 cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga
     9841 agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga
     9901 acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa
     9961 accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa
    10021 gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc
    10081 actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca
    10141 gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc
    10201 ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg
    10261 aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg
    10321 tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt
    10381 tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt
    10441 attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc
    10501 ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg
    10561 ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct
    10621 gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga
    10681 agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc
    10741 agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg
    10801 attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac
    10861 aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc
    10921 ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga
    10981 tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga
    11041 aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa
    11101 gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat
    11161 cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa
    11221 tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc
    11281 accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat
    11341 cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt
    11401 tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa
    11461 cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta
    11521 atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc
    11581 gggcagattt taattgattc atctggtact cttttttatt gtccttacat tcataaagtt
    11641 tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat
    11701 tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata
    11761 tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa
    11821 tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga
    11881 gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca
    11941 taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag
    12001 ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa
    12061 ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg
    12121 atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc
    12181 aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt
    12241 gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca
    12301 aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg
    12361 acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt
    12421 tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc
    12481 ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca
    12541 gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac
    12601 taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg
    12661 caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat
    12721 tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat
    12781 tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa
    12841 aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg
    12901 ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt
    12961 atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga
    13021 caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg
    13081 tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg
    13141 aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt
    13201 ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa
    13261 aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga
    13321 tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag
    13381 cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga
    13441 tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt
    13501 atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga
    13561 agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga
    13621 gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt
    13681 tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat
    13741 ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt
    13801 taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga
    13861 taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa
    13921 caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat
    13981 aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag
    14041 taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa
    14101 cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa
    14161 atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac
    14221 tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa
    14281 gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa
    14341 attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc
    14401 agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca
    14461 cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg
    14521 catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt
    14581 tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat
    14641 gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt
    14701 agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt
    14761 cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt
    14821 ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga
    14881 cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc
    14941 aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt
    15001 ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc
    15061 tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct
    15121 tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc
    15181 agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt
    15241 aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg
    15301 ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga
    15361 actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga
    15421 caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag
    15481 gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag
    15541 atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag
    15601 ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg
    15661 ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg
    15721 atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga
    15781 atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg
    15841 tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag
    15901 gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag
    15961 cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga
    16021 aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat
    16081 tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt
    16141 atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg
    16201 atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga
    16261 tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg
    16321 catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc
    16381 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt
    16441 ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt
    16501 ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac
    16561 aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc
    16621 acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag
    16681 ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa
    16741 attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa
    16801 tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat
    16861 tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat
    16921 ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt
    16981 ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac
    17041 agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt
    17101 ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat
    17161 attattctca aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc
    17221 acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg
    17281 atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg
    17341 cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga
    17401 taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg
    17461 gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca
    17521 aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa
    17581 tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt
    17641 tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct
    17701 tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt
    17761 ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc
    17821 ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga
    17881 gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta
    17941 ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct
    18001 tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca
    18061 atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt
    18121 aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca
    18181 ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca
    18241 ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc
    18301 atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata
    18361 tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca
    18421 gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga
    18481 ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa
    18541 ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt
    18601 tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa
    18661 cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc
    18721 tttaataaat atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca
    18781 atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct
    18841 atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct
    18901 accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa
    18961 tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat
    19021 ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca
    19081 cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag
    19141 gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata
    19201 gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg
    19261 cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt
    19321 ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat
    19381 agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct
    19441 aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca
    19501 ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg
    19561 gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc
    19621 ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt
    19681 gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt
    19741 agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt
    19801 tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc
    19861 cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct
    19921 tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa
    19981 aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc
    20041 ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc
    20101 catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt
    20161 aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat
    20221 ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac
    20281 tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat
    20341 cgactgagct ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag
    20401 caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag
    20461 tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat
    20521 cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac
    20581 tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt
    20641 ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac
    20701 gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg
    20761 tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag
    20821 aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg
    20881 catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac
    20941 cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa
    21001 actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct
    21061 tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac
    21121 gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat
    21181 cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga
    21241 ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct
    21301 gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg
    21361 gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg
    21421 aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg
    21481 aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat
    21541 aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg
    21601 ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta
    21661 aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt
    21721 ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc
    21781 cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag
    21841 gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc
    21901 ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc
    21961 ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg
    22021 gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa
    22081 atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg
    22141 cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt
    22201 cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc
    22261 ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg
    22321 aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg
    22381 ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc
    22441 ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt
    22501 tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga
    22561 aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga
    22621 agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac
    22681 ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac
    22741 aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg
    22801 ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac
    22861 attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata
    22921 attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc
    22981 aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc
    23041 tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat
    23101 gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa
    23161 agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat
    23221 tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt
    23281 tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg
    23341 taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca
    23401 aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg
    23461 gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc
    23521 gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt
    23581 tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa
    23641 aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt
    23701 tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa
    23761 aaaaaagaaa atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta
    23821 atttttctat aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac
    23881 tctatttact tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa
    23941 aaagtgggtt gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat
    24001 atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg
    24061 gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa
    24121 aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt
    24181 ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa
    24241 aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca
    24301 ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca
    24361 ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca
    24421 agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac
    24481 gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag
    24541 atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg
    24601 atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa
    24661 ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct
    24721 ataataagat atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt
    24781 tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga
    24841 accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag
    24901 ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg
    24961 agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg
    25021 aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg
    25081 aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca
    25141 gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac
    25201 cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc
    25261 agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg
    25321 cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag
    25381 cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct
    25441 tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc
    25501 ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac
    25561 gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa
    25621 gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag
    25681 aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca
    25741 gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga
    25801 aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa
    25861 tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata
    25921 cagcacagca aggaatctat attacgcaag accagacctt agagcaatta atggagaagc
    25981 aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga
    26041 aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat
    26101 ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga
    26161 cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg
    26221 gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac
    26281 cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt
    26341 atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata
    26401 ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt
    26461 caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca
    26521 gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc
    26581 cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg
    26641 atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt
    26701 tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag
    26761 caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga
    26821 ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag
    26881 cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag
    26941 aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa
    27001 aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca
    27061 ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg
    27121 aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa
    27181 tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta
    27241 ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc
    27301 agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc
    27361 tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga
    27421 agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc
    27481 attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag
    27541 atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat
    27601 tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga
    27661 tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag attcattaga
    27721 gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt
    27781 tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt
    27841 tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga
    27901 tcatgtctat ctcgttgccg gattgaattt gccgctattg attgagctag tatctcggtt
    27961 ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat
    28021 gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga
    28081 agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt
    28141 tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg
    28201 aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc
    28261 aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta
    28321 ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc
    28381 aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg
    28441 gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa
    28501 gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa
    28561 aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca
    28621 atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac
    28681 aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc
    28741 atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat
    28801 tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg
    28861 tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc
    28921 aattatgaac cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca
    28981 tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc
    29041 ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca
    29101 aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg
    29161 tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta
    29221 cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt
    29281 caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga
    29341 tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt
    29401 ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg
    29461 tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag
    29521 ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag
    29581 gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga
    29641 tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt
    29701 ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata
    29761 ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc
    29821 tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg
    29881 gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa
    29941 tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg
    30001 tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg
    30061 gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag
    30121 ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg
    30181 tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag
    30241 aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg
    30301 ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt
    30361 actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc
    30421 gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa
    30481 atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt
    30541 gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt
    30601 acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg aattggaata
    30661 agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata
    30721 atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt
    30781 ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct
    30841 aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta
    30901 gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt
    30961 gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac
    31021 gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta
    31081 gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc
    31141 gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt
    31201 gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa
    31261 gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc
    31321 gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat
    31381 gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta
    31441 cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt
    31501 attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag
    31561 aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta
    31621 tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca
    31681 gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag
    31741 ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc
    31801 ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat
    31861 caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca
    31921 tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca
    31981 accccagtca cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct
    32041 aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta
    32101 gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg
    32161 acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc
    32221 tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag
    32281 agcagcgata actgctccct ttattttttt aaaatattgg catcaattac gttataaaac
    32341 acattttttg cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc
    32401 aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc
    32461 caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac
    32521 aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt
    32581 tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc
    32641 taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa
    32701 gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt
    32761 caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac
    32821 tttaagaaat gtatactata atggaaatta tcaattattt atttggagga atatccatga
    32881 aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg
    32941 cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca
    33001 ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg
    33061 gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa
    33121 caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca
    33181 ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg
    33241 ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg
    33301 ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag
    33361 cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg
    33421 atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg
    33481 ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg
    33541 cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga
    33601 gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg
    33661 gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg
    33721 ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga
    33781 tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag
    33841 caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag
    33901 atgatcaata gttaatcgt
//
LOCUS       DACECB010000026        31694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECB010000026 DACECB010000000
VERSION     DACECB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.026"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IWO04_001307"
     CDS             363..731
                     /locus_tag="IWO04_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0212929.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IWO04_001308"
     CDS             911..1852
                     /locus_tag="IWO04_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0212930.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IWO04_001309"
     CDS             1921..2652
                     /locus_tag="IWO04_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0212931.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IWO04_001310"
     CDS             2796..4976
                     /locus_tag="IWO04_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0212932.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IWO04_001311"
     CDS             4996..5478
                     /locus_tag="IWO04_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0212933.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IWO04_001312"
     CDS             5494..6255
                     /locus_tag="IWO04_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0212934.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IWO04_001313"
     CDS             6386..7720
                     /locus_tag="IWO04_001313"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0212935.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IWO04_001314"
     CDS             8027..8512
                     /locus_tag="IWO04_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0212936.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IWO04_001315"
     CDS             8505..9188
                     /locus_tag="IWO04_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0212937.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IWO04_001316"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IWO04_001316"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0212938.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IWO04_001317"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IWO04_001317"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0212939.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IWO04_001318"
     CDS             12804..13433
                     /locus_tag="IWO04_001318"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0212940.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IWO04_001319"
     CDS             13485..13817
                     /locus_tag="IWO04_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0212941.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IWO04_001320"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IWO04_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0212942.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IWO04_001321"
     CDS             14643..16016
                     /locus_tag="IWO04_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0212943.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IWO04_001322"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IWO04_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0212944.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IWO04_001323"
     CDS             17071..17511
                     /locus_tag="IWO04_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0212945.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IWO04_001324"
     CDS             17655..18356
                     /locus_tag="IWO04_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0212946.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IWO04_001325"
     CDS             18353..19474
                     /locus_tag="IWO04_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0212947.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IWO04_001326"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IWO04_001326"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0212948.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IWO04_001327"
     CDS             20853..21761
                     /locus_tag="IWO04_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0212949.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IWO04_001328"
     CDS             21796..22068
                     /locus_tag="IWO04_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0212950.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IWO04_001329"
     CDS             22079..23125
                     /locus_tag="IWO04_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0212951.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IWO04_001330"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IWO04_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0212952.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IWO04_001331"
     CDS             complement(26136..26807)
                     /locus_tag="IWO04_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212953.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IWO04_001332"
     CDS             complement(26800..27294)
                     /locus_tag="IWO04_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0212954.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IWO04_001333"
     CDS             complement(27314..28534)
                     /locus_tag="IWO04_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0212955.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IWO04_001334"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IWO04_001334"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0212956.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31692)
                     /locus_tag="IWO04_001335"
     CDS             complement(30610..31692)
                     /locus_tag="IWO04_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0212957.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10548 a         5658 c         6786 g         8702 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atat
//
LOCUS       DACECB010000027        30567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECB010000027 DACECB010000000
VERSION     DACECB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.027"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(297..2372)
                     /locus_tag="IWO04_001336"
     CDS             complement(297..2372)
                     /locus_tag="IWO04_001336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0212958.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2374..3291)
                     /gene="glyQ"
                     /locus_tag="IWO04_001337"
     CDS             complement(2374..3291)
                     /gene="glyQ"
                     /locus_tag="IWO04_001337"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0212959.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3677..4489)
                     /gene="recO"
                     /locus_tag="IWO04_001338"
     CDS             complement(3677..4489)
                     /gene="recO"
                     /locus_tag="IWO04_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0212960.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4632..5531)
                     /locus_tag="IWO04_001339"
     CDS             complement(4632..5531)
                     /locus_tag="IWO04_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0212961.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5546..5947)
                     /locus_tag="IWO04_001340"
     CDS             complement(5546..5947)
                     /locus_tag="IWO04_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0212962.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5925..6401)
                     /gene="ybeY"
                     /locus_tag="IWO04_001341"
     CDS             complement(5925..6401)
                     /gene="ybeY"
                     /locus_tag="IWO04_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0212963.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6418..8610)
                     /locus_tag="IWO04_001342"
     CDS             complement(6418..8610)
                     /locus_tag="IWO04_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0212964.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8634..9605)
                     /locus_tag="IWO04_001343"
     CDS             complement(8634..9605)
                     /locus_tag="IWO04_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0212965.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10311..11690
                     /gene="brnQ"
                     /locus_tag="IWO04_001344"
     CDS             10311..11690
                     /gene="brnQ"
                     /locus_tag="IWO04_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0212966.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11846..12292)
                     /locus_tag="IWO04_001345"
     CDS             complement(11846..12292)
                     /locus_tag="IWO04_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0212967.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12319..12495)
                     /locus_tag="IWO04_001346"
     CDS             complement(12319..12495)
                     /locus_tag="IWO04_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0212968.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12657..13085)
                     /locus_tag="IWO04_001347"
     CDS             complement(12657..13085)
                     /locus_tag="IWO04_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0212969.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13177..14037
                     /locus_tag="IWO04_001348"
     CDS             13177..14037
                     /locus_tag="IWO04_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0212970.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14131..14424)
                     /locus_tag="IWO04_001349"
     CDS             complement(14131..14424)
                     /locus_tag="IWO04_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0212971.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14497..16224)
                     /locus_tag="IWO04_001350"
     CDS             complement(14497..16224)
                     /locus_tag="IWO04_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0212972.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(16438..17364)
                     /gene="trxB"
                     /locus_tag="IWO04_001351"
     CDS             complement(16438..17364)
                     /gene="trxB"
                     /locus_tag="IWO04_001351"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0212973.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17509..18942)
                     /gene="ascB"
                     /locus_tag="IWO04_001352"
     CDS             complement(17509..18942)
                     /gene="ascB"
                     /locus_tag="IWO04_001352"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0212974.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18942..20345)
                     /locus_tag="IWO04_001353"
     CDS             complement(18942..20345)
                     /locus_tag="IWO04_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0212975.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20575..21477
                     /locus_tag="IWO04_001354"
     CDS             20575..21477
                     /locus_tag="IWO04_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0212976.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21518..23404)
                     /locus_tag="IWO04_001355"
     CDS             complement(21518..23404)
                     /locus_tag="IWO04_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0212977.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23501..24352)
                     /locus_tag="IWO04_001356"
     CDS             complement(23501..24352)
                     /locus_tag="IWO04_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0212978.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24687..26471)
                     /locus_tag="IWO04_001357"
     CDS             complement(24687..26471)
                     /locus_tag="IWO04_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212979.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26471..28222)
                     /locus_tag="IWO04_001358"
     CDS             complement(26471..28222)
                     /locus_tag="IWO04_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0212980.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28364..28588)
                     /locus_tag="IWO04_001359"
     CDS             complement(28364..28588)
                     /locus_tag="IWO04_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0212981.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28839..30425
                     /locus_tag="IWO04_001360"
     CDS             28839..30425
                     /locus_tag="IWO04_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0212982.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8542 a         6593 c         5457 g         9975 t
ORIGIN      
        1 aaactaatat attcatagaa atatttataa acaggttttc cctatataat cataccacaa
       61 aaagttttct tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatca
      121 tgattaaccg ttttcatttt tatttcctct cttttcatga gcattttgaa aattcgaaca
      181 aagaataaaa taacaaaaat tattaaaata gaattaataa cagctattta tatttataac
      241 aaaaaaacag agacacagca cgtttcgtgc atgctctctg tttttcaaac aaaagactat
      301 tttgtgatga tttgatccaa gcttgctaat gacaaagcca tggcagctat ttgactaagt
      361 tgtgttagtc ggttcttacg tactgctggt tcatcagcca tgaccatcgt ttgatcaaaa
      421 taattttcga tcagtggacg aagagcgaca agctggtcgt agttttcgtg gatcgtttgg
      481 tcagcaaaac ttgctttcat tgcttctact gctttgtgta attctttctc tgcgtcgttt
      541 tcgaacagct caggatctac ttctgattgt ttttcaagct gcgctttttt cgctaagttc
      601 acgacacgtg tcaaagcttc cattgaaggt ttgaagtctt catctgcaag atgttgtttg
      661 aacaattgtg ctgctgcaaa tacttttgtc agatcttttt gttccgcatt caaaacagca
      721 tcgatgacat catgacgaat attcttcgtt gtcaataatt ggcgcagacg gcctttgata
      781 aagtcgatca cttgttgttg cccttctgat aaaccaatgc cgaaacgatc cgtatcttta
      841 ctgataacat cgctaatcgt ttcttgcaga acagacaaag gaaaggccca ttttttcgat
      901 tcgatgatcc gaacgacacc gtatgcttgg cgacgtaagg cataaggatc gtttgaaccg
      961 cttgggatca tcccaacagc aaagaaagaa aagatgctat ctaatttatc tgcaatcgct
     1021 aaaacagaac ctactgtaga ctgaggtagc tctccttcac tggatatagg catgtaatgc
     1081 tcacgaatcg ctgtcgctac agcaggtttc tcgcctttca gcagagcata tttttcgccc
     1141 atgatccctt gcagttcagg aaattcccct accatgttgg ttactagatc aaatttatag
     1201 atttcagcag ctcgttgcag atccatcaat tcttcctcag atagtcctac ttgttcgcca
     1261 atgatacggg caatgacttg tacccgttgc attttctcat agatactacc gattttttca
     1321 tggaaagtaa cagtttttag tttttcaacg tattggtcga ttgaagcttt cttgtcttca
     1381 ttataaaaga attccgcatc ttctaaacga gccgtcaata ctttttgatt gcctcgaacc
     1441 acattttcca attttacccg atcaccatta cgagcagaaa taaaatgagg aagcagcata
     1501 ccttgatcat tccgtacctc gaaataacgt tgatgttctt tcatagaagt gactagtact
     1561 tcttcaggaa cagaaagata tttttcttcg aagtcgccag aaaagacagt tggatattcg
     1621 actaaattgt tgacttcttc tagtaaatct tcgtctagat caataaccca atgatttttt
     1681 tcggctaatt cattggcttg tgtaacgatc atttcttgtc ttttttgcgg agacgcaatg
     1741 acgaattgtt cattcaattt ttcttcatat tcttttgcat gcgcaaatgt cacatcttcg
     1801 cctaagaaac gatgacctcg agaggctctg cctgttttga catccaagat ttcgaatggg
     1861 attacttcgt catctaacaa tgccacgatc cagtgaatag ggcggatata ttcaaaatcg
     1921 tattctgccc aatgcatcgt tacagggaaa gtcaggcttt taatgacatc tgctaaacct
     1981 gtcaatactt ctttggattc cttaccatga gtgtatttcg ttacatagac atattccaca
     2041 ccttttaatt ctctaaaagt gatagcgtct gtcgtcagac cttgtccgcg gacaaatcct
     2101 tccgctgctt tcgtccagtt tccatctgcg tccaaagcaa tttttttggc tggtcctttg
     2161 acttcttctt cactgtccgc ttgtcgttct ggaatgtttg ttactttgat tgctaaacgg
     2221 cgcggtgtgg aaaatgtttc gatcgtatca taagtcaatt ggttttcatc taaaaatttt
     2281 gctgtttttt cttctaattg tttcatgctt ggtgtgacaa catgagcagg catttcttcc
     2341 agcccgattt caagtaataa gtcttttgcc atcttattca gcctccttca cgttttctgg
     2401 atttgctgtg tttttattca acaaagggaa tcctaatttt tcgcgttctg ccacaaagat
     2461 tttggctaca gcacgagcca tgttgcggat gcgagaaaga taaccggcac gctctgttac
     2521 ggatactgca ccgcgggcgt ctaataagtt gaatgtatgg ctacatttca agatataatc
     2581 atacgccgga tgaacaagat tttcatcgat acagcgttta gcttcttttt caaaacgatc
     2641 aaagttgctt agtagcaagt cttggtcgct tacttcaaaa gaatatttag aatgttcata
     2701 ttctggttgt ttgaagatct cgccatattt cacgccatca gcccattcta aatcatagac
     2761 actttcaact tcttggatat aagaagcaag acgttctaat ccataagtga tctcagaagt
     2821 tactggttta caaggcagtc caccaacttg ttggaagtac gtgaattgtg tgatttccat
     2881 tccgtcaagc catacttccc aacccacacc ggcacagccc atggatgggt tctcccagtt
     2941 gtcttcaaca aaacggatgt cgtgttctag tggatcgatt cctaaaaggc gcaagctttc
     3001 taaataaagc tcttggatat tctcaggtga aggtttcatc acgacttgga attgatgatg
     3061 ctgatacaaa cggtttgggt tttcgccata acgtccatca gctggtcggc gagatggctc
     3121 tacgtaagca gcattccatg gttctggtcc gattgcccgt aaaaaggtat atgggctcat
     3181 cgtacctgct cctttttctg tgtcatacgc ttgcatcagc atgcagccgt ttgatgacca
     3241 aaatttttgt aatgttaaaa tcatatcttg gacggtcaat tttttactca tctgttttgc
     3301 ccctttcaat tttaaagcgt tgctgtcaaa taagaataca ggaaagaaaa cgacaaaaaa
     3361 tccctatgtc tgattacaga catagggacg aataattcgc ggttccaccc tacttccgaa
     3421 taaagattcg gcagcttcat ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt
     3481 ccggctccca ctctcccgaa ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt
     3541 acgtattcat tttgcagttt tcttgtttct ataatagatc ctctcagtga acgatctatt
     3601 tgatctacgg ttaataatag acggaactcc ctgtgtttgt caaatggttt tgattttttg
     3661 ttgtatgttt catttactat tcttcttttt taggtagatc tttttcagac atgtctttct
     3721 ctgacgtctt taatacgtgt gcccaagact tcatttgatc aatgaacttt ttgcttttca
     3781 aatggatacc gacatattca tcatacagct ggtcgatgag ttcacggatt gcttgtttgg
     3841 tttcttcttt cagatggatt ccactgattt tatcataaga aatagctgaa aacatccgaa
     3901 cgaaataaac agctcgagga tctgcatgat aacggtgatc atccatcgac cagtgtcttt
     3961 cgcatatcac accaccatat ttggaagaat agtcaaattt tccttttgtt tccccacata
     4021 cgacgcagtg tgtccagatt ggtgcaattc cgaagcgttg cagtatttgg acttcaaaaa
     4081 tattcgtgat gatttctgca tcatctcctt ggtccatcag ttgcagtgct tgttgcgtaa
     4141 actggaacaa atgcgggtcg tactgatgat cttcgattgc cgcatctact agattcaata
     4201 aatatgtgcc atagccgctc aaaaagatgt cttcttggat gtgtcgaaac gcgtggacat
     4261 ctttgacact gcttaaaaat gacaaacctt cacttcgaaa attcccgata taaactgctt
     4321 cagtaaaagg gagcaaagcc ggagctaagg gctgattttg ccgatggatc cctttgatat
     4381 aaaacatcat tttgccagaa gattccgtaa agattttgac taatttatct ttttctttgt
     4441 aattacggct agataaaatc aagccttttg attctgcaag ctggctcata ttctccctcc
     4501 taaaaaataa atctggaaaa gcagattctg ccgatccaga tcatttatgt tgcgatcttc
     4561 cgcagctttt ctcaacacgc ggatttttat ccagtttatt tctataccta acttacaaga
     4621 agctcagaat atcaatattc gtcttttcgg tagccgaagt cttgcagata gacttttttg
     4681 tctcgccagt ctttttgaac tttgacccaa agttctagaa agactttgtc tcctaaaaga
     4741 ttttcgatat cttttctcgc tttcgttcct acttgcttca gcatttttcc gcctttgccg
     4801 ataatgatcc ctttttggct atctctttcg acaataatcg tagcctggat atggatttta
     4861 tcattctcgt ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct
     4921 cgtgttaaaa acaagacttt ctcacggatc aattcagaaa cgatgaagta ttcaggatga
     4981 tcagtcactt gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca
     5041 tccatcaaac gttcgacgtt gtttccttct gtagcagaaa ttggaatcac ttgtgcaaac
     5101 ggcatctgcg ttgaataatc ttcgatgatt cccaacaact cgtctggatg gattttatcg
     5161 attttattga taatcagata aacaggagaa tgcacgtttt ttagacgttc aataataaaa
     5221 tcgtctcccc gaccgcgttt ttgatcagcg ctgatcataa ataagaccgc gtccacttct
     5281 ttcaacgcac tgtaagctgc ttctaccata aaatctccta aacgatgctt tggtttatgg
     5341 attcctggtg tgtcaataaa aatcaactga gcttctggtg tagtatatac tccttggatt
     5401 ttatttctag ttgtctgcgc cttatcactc atgatggcaa ttttttgacc cacgattcga
     5461 ttcaataatg tggattttcc tacattcggg cggccgacga ttgcgacaaa ccctgattta
     5521 tgttctggca tatacgttct cctttttaat taaacaacaa atgatatatt ttagggacaa
     5581 ataaaatcaa cccaacgatg acggaaaaac aagcggtcac taaaactgct ccagctgcca
     5641 tatctttgat ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccacgacat
     5701 tttcaaaaac agtattgatg atctctacta tccatacgag aaaaacagct aaaagcagcc
     5761 ataaccattc tatccgatca agctggaaaa tcacgcccat aaacaaagcg aggattccga
     5821 acgcaacatg tttcctcatg ttgcgttctt cctcaaaaac ggttttgact ccttggacgg
     5881 caaattccac ggacatcagg aaatgtttgt ttttctctac gtttttatct tttaagtcca
     5941 taggcatcta agatctcttt ctgtaaacca aacatttctt tctcatcctc tggtgtcata
     6001 tggtcatacc cgttgatatg caagaatcca tgaaccgcta aaaagccaag ttcccgatca
     6061 tacgaatggc cgtaagcttc tgcttgctct gcagcacgtt cagtagagat catgatgtct
     6121 cccagtacac gaggcaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg
     6181 ccctcttctt ccaaggcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca
     6241 cgattgatta cttggatcgc tgcattgtcc atgaaagtaa cagacatttc tgtttcttct
     6301 ggcaactcta aatatgatgc tgcaaattgc agcaagtcgt cgatttcttt cattttctct
     6361 tctgagactt ggtttgtttc atccatgaaa gtaatatcca ttatacatcg cttctttcta
     6421 ttcttcttgt tcttctttta tcttctcaac ttccgatttc attttaggat atttgatccg
     6481 agaatggaag gtactgctta aaccgctgat caatgatttt tctactttac ggatttcttt
     6541 taacgtcaat ccactgtcgt ccaattgtcc atcggaaata cgctcttcaa taagattatg
     6601 gacaaaactt tcgatttttt cgctcgttgg atggtccatt gctcgtactg ccgcttcaca
     6661 actgtcagct atattgacga ttcctgcttc acggctttga ggttttggtc caggataacg
     6721 gaattgctct tccgtgactt ccgggttgcg ttcctttgcc ttaacgtaga aataacgcat
     6781 cagcgtggtt ccatgatgct gtctgcaaat atcgatcacc atttgcggca tattataatc
     6841 ttccaatatt ttggcaccat ctgtcacatg tccaaagata atatgtttac tgtcttctgg
     6901 caataagaaa ttgtgcggat tttccgctcc tgaaggcaag ttctcaacga aaaagctggc
     6961 gtgtttgatc ttgccgatat catgataata gcaagctact cgagttagta aagaacgtcc
     7021 accgatttct gctacagcat tagcacttag attggctacc atcatgctat ggtgatacgt
     7081 gcctggtgct tcttccagca actgtttcaa taaaggatga ttaggattgc tgagttcatt
     7141 taatacgatc acactatcat cattgaccat caattcaata taggggtgca gacctatcgt
     7201 cagcaagaat gagaacagtg cacctgtgaa tccgcatact agcgttaacc aagtcgttgc
     7261 atccccaaag ctcattcctt ggtagataat caaaatgaca tccatcatca caggaaaaat
     7321 aatgacccac atagcagcgg ataacccttg ttctgagatc cgtttgcgtc tgacgatcgt
     7381 tgccatgaga ccagaaaata cataggacat caaaataact gtcagaaagt tcgtgccaat
     7441 cgaatcataa aaaatgaaaa gtgcggatac tgcttgaaaa ataccagcca tgatccctgc
     7501 tcttcgattc aagaaaaagc ttaaaacaag tggagcaaaa gccgcggggt aaaacagcgg
     7561 aatataagca gcttgttctg tttggaagag ttggaaaaat ttcattagta cgacgcttac
     7621 tgacattgcc gtcacgtaga aaagtacgta ttttgttctt tctcctgcat tttggaattg
     7681 aagtgaatta taaatcagta caccgatttg aagcaaaaca gctaaaacca tggcaatgag
     7741 tgggaaaata gaagtcgttt gattcgtcaa tcctagtaat tccaattttt tcatcgctga
     7801 agcatcaatt tgatttcctt cgcgaacaat aatttctcct tggaagatca tcactggttg
     7861 tacagcttct cgtgcagatt gttttaattc ttctgtcttt ttctcattca atacatcatt
     7921 aactacaatg ccttgatcca ctaagtagcg aatcatttgt tgctgctctg ttgtgacatt
     7981 caaatattga atctgatctt ctgcttcttg tttaaaagct tccaattcgc tttcacgaac
     8041 ttgtttactc atctgttcgt caatgagctt caaactttca tccctaaccg tgtgaatttc
     8101 cgtttcagtc atttcaaata tcgtcgaata aaaattgttc ggcagttttt ggtaaaacgc
     8161 aacgttttct gcattcacgt tttcaaaatt tttcttcagt gtagcaatcc gctcttctgt
     8221 agtaggtttc gggatatttt catcttcctt cgccttatct accttgtcat tataagattt
     8281 gttgatggta tcattcgtct tatcgatcaa ttcgaataat tgcttgatcc gattgttctg
     8341 gtcatctgcc agatcttttt gataagtata ttctggagtc actgcttctg ctgccagttt
     8401 tcttctttgt tcggtttctt ccgtgttctc aatcgtttta ttcgcacgga tactctcttc
     8461 tgctacttgg ccttctctga aatcattggc cttttgtttg acactactaa atgtaacgcc
     8521 gcagacaatg ataaaaaaca agaacagaag gaaaggcaat gctgcctttc cgagtttttt
     8581 ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc tcctaagcaa agcttattct
     8641 tgatgaagat ccgccttttc ataagctttg atgatttcgg caactacagg gtgccgaaca
     8701 acgtcacttg cttcaaaatt aacaaaatcg atttttttga tttgctttaa tgttcgttct
     8761 gcatgaacaa gaccacttgt cgtcccacga ggcaagtcta tctgacttgt atccccattg
     8821 acaatcattt ttgattgata accgagacgg gtcaagaaca ttttcatctg agcgactgtc
     8881 gtgttttgtg cttcgtccaa gataacgaat gcatcatcca atgtccgccc ccgcatgtaa
     8941 gctaaaggtg cgatttcgat cacgcctcgt tccatcaatc ggttcgtatg atccaatcca
     9001 aaaatttggt ataaagcatc ataaacaggc cgcagataag gatccacttt ttctttcaga
     9061 tccccgggta gaaagcctag gttttcccct gcctccacag cgggccgcgt caaaatgatt
     9121 ttttgcactt cgcctttttt tagggcagca attgctaata ctacggcaag aaacgttttc
     9181 cctgttccgg ctggccctac accaaatacg atgtcatgtt tggctaccga ttcaacgtat
     9241 ttcttttgac cgctgttttt tacccgtata ggttttccat tacggtcttt gacgatctct
     9301 tcctcataca tctcaacaaa ataatctaat gtaccttttt gccccatctt caatgccgtc
     9361 acaacatctg gcgtacttac gtggattcct cgtttgatca attcctgtaa tgtccgaatg
     9421 actgaggcag ccaaggaaat ctgggattgc tcaccaatga tctgaatgat ttctccacgt
     9481 gtatggatgg ttgtttccgt tgtttcctcg atcagtttta tatgcttgtc gtgtgcacct
     9541 aaaagcatgc tgacatcatc agcagctgtc aatctaatct ctaaagaact agactcttcc
     9601 gtcaaaaata gacatcccct ttttttcgat tcattctttc ctattttacc atactcagaa
     9661 gaaactatat agcaaataag aaaaggttgt aaaagagtcg tttaaccata cgtaccaaga
     9721 aacgttaaaa aatagccgtt tccattcgct aaaggtctcg ttttgataaa aagaagctga
     9781 gaatgtaagt tatatgaaag tggagaacaa aatattccgt ttcattcagc aggtattctc
     9841 taggtatttt tcttgtttca aaaaaagaaa acaaaatagt taaaatattc ttgcatttca
     9901 aaaaaaacag gtgtattatt acgtcaacaa ataaacatac agcaaagcgt cgaggaaaac
     9961 agtaggctat tcaacgattt attcccagtg agtcatgtaa gtgagaaatg acaataaatc
    10021 gaatagacga acatcatttc cgagtcaatg tcgaaaacgt agatcattgt ggctgcctcc
    10081 attatcaggc tagcggataa ctttatcaaa aagcgtccgt gaatgaagaa gcttttttaa
    10141 gcgaaaaaag gtggtaccgt tctagaatct agagcccttt tcccaagtgg aaaagggctt
    10201 tttttatatt ttaaggtggt ggttttcatg gaatctgata ttagtggcat tactaattgc
    10261 tgcaacgttt atatcgtcaa tcaaaatgat aattattgga ggaaaaattt atggaagaaa
    10321 caaaaactta ttcatggaaa caactattga tggtcagttc acttatcttc gggatgttct
    10381 tcgggtcagg taatctaatc tttcctgtcc acttaggtca aatggctggc gcaaactggg
    10441 tcagtgctgg cgtaggattt gccatctcgg gcgctctttt cccattacta gctatcttag
    10501 ctgtagtagt gacaaaaagt gatggtctgt atgacttagc taaacctgtc ggaaaatggt
    10561 atgctgcctt gttcttaatc cttgttcatt tgacgatcgg accatttttc ggtacaccac
    10621 ggacagctgc tactgctttt gaaatggctg ctaaaccatt tttgcctgaa aaatataatt
    10681 cattaggtat gtttttattc tctgctcttt tctttttact tgcttatttc cttactgtcc
    10741 atcccacacg gttggtcaaa tacgtcggaa agtatctaaa ctccttgttt cttgcattgt
    10801 tagcaatcat ttttgtcgtt tctttattcc acccaatggg aaatctaaat caatctgctg
    10861 cagatactta ccaaacaaac gctttaacaa atggtgtcct agaaggttat aacacggttg
    10921 atgcagtagc cttgcttgcc ttaagtgtca cttttgtcca tgcagtccgt gaccttggtt
    10981 tcaaagataa aaaagtcaca gaattaacag caaaagctgg tagcctgagc gttgcgctag
    11041 aagtcatgat ctattttggt cttgttcttc tcggtgcgat gagtttgaat aagatgtctt
    11101 tatcagaaaa tggcggaact gcactttctc agatcactaa tttttattta ggtaacttcg
    11161 gcgctagctt cttaggagtg atggtcacat taggtgtctt cactaccgct atgggactcg
    11221 ttgtttcttt tgcgcaagat ttccataaac tgttccctaa agtcagttat atgacatggc
    11281 tgcggttaac gactttcgtc tcttttgtcg ttgcaaatgc tggtttagac aatatcatcc
    11341 aatggtcttt accagtattg atgcttttgt atccattgtc gcttgcgttg atcttgcttt
    11401 ccttaacagc aaaatttttc caaaagacac catttgtcta tcaagtaacg atgttgtttg
    11461 ctgctgttcc tgccgtattg gatatgctgg caaattcgcc tgctttagtg agtcaacaaa
    11521 gagttgtagc aagcatgctc gaattttatc atcatcacgt tccttttgct gcattaggac
    11581 ttggctggat ggtcccaacg ttacttggtt acgctggcag cctactgttt tattacgcct
    11641 atcgcctatc agggtataaa caagaagcaa atgaactacc agaagaataa ccaaatcaaa
    11701 cacaatttaa cataagaaat attaaacagg aaaaaaccag ctagatgaaa gaatcctatt
    11761 ctttcgtcta gctatttttt tgttctcttt tttacgcgca caaaaaaact gcggacaatg
    11821 attgtccgca gtttaaatga taaaattaag attgtaatag ttctttcaca atggcattta
    11881 cttggtttcc atctgctttt cctttgactt taggcatgat cgcacccatg actttgccaa
    11941 attctttcat tgatgaagca ccagtttgtg tgattgcttc ttgaacaagt tgtcgaattt
    12001 cttcgtcgga aagttgttcc ggcatgtatt tttcgactat cacaatttca ctttttactt
    12061 tttcagctaa atcgtcacgt cctgcttctt caaactcatg caaagaatca cgtcgctgtt
    12121 tcatttcgcg agacaacacc gttaattctt cttcttctgt caagtcgtga ccttctttaa
    12181 tctgctcgtt ttgtatagat gacttgatca ttcggatgac ttgtaaagat tccttatctt
    12241 tcgctttcat cgctgttttc atgtcatcgt ttaatctact tagtagtgac acactaccaa
    12301 ctccaattca tcacagaact agaatttttt gcgttttcta gctgcttcag attttttctt
    12361 gcgtcttaca cttggttttt cgtagaattc acgtttacga gattcttgta aagtacccgc
    12421 ttttgacacg gaacgtttga agcgacgaag agcatcgtca agtgattcgt ttttgcgaac
    12481 gactgttttt gacatgttaa ttccctccct ccgagcttaa aaagtaaccg taattgttat
    12541 tgaaatatat ctcaaaaaac aagactgtcc tgtaaatatt atatctaatg ctgttttgac
    12601 cgtcaaggaa atcttgcttt tttttaaaat tatcaaatgt tattttattt aaatccttat
    12661 tgacaatttt cacatcttcc gaaaatctcg aatctatgcc cttcgatcgt acatccaccg
    12721 agctgttctt cgaaataatt cattggacac atatggattt ctttcgttgt tccgcacacc
    12781 gtacagataa agtgatggtg atggccgatt tccccttgac agcaatgaaa acgaaacttc
    12841 atctctccat ttaaatcggt ttcttccagt aaatcgattt ctgcaaaatc atggagattt
    12901 cgataaatcg tatcgtaact tactcctttg aattcctgat tcatatagtc aaaaacttct
    12961 ttagcggata cgtagcgatt gcgcttcact aaataagtta acaatgcttc tcttttttta
    13021 gtatatttaa aaccgttatt tttcaatgtt tctagtgatt gttcaatcaa tgtctgtttt
    13081 ttcatgactt attcctccaa atcaaaatga ttacgaagta cacattctag agtatggtat
    13141 aataaacata aaaaaaatgc aagtgaggtt ttatccatgt cttccgattc tacagaaatc
    13201 gttacttttt ttgtcatctc tgactctgct ggagaaactg cgacaaaatt agcgcaagcc
    13261 actatggccc aatatcctac cgttgagttc aatttattcc gcagaacatt tgtgacagat
    13321 gagaaaacat tgatgcaggc actgcaagat gcactaaaag aaaaagccct tgtcctgcac
    13381 acattgatca atcaagaatt gatcgagatg acgcgttctt tttgtgaaga acatcaatta
    13441 tttagttttg atgtgatgac tccgccgata tcagaaatcg agcgcttaac tggtatcaag
    13501 cccattcggc aacctggcgc ccttcattta ttgaatgaga actatttcaa acggatcaaa
    13561 gcaatggaat ttgctgtaaa atacgatgac gggaaagatc ctcgtgggtt tctagaagca
    13621 gatgtcgttt tgctaggcgt atccagaact tccaaaacgc ctttgagctt gtttttagct
    13681 aacaaaaatt taaaggttgc caatctacct ttgatcccac aagcacatat tcccaaacag
    13741 ctttgggaga tcgatcctaa aaaaatcgtg ggactgacga ataacccaga tattttaaac
    13801 aatatccgta aagaacggat gcgctcttac ggattaaatc ctgatactgc ttattccgat
    13861 atcgaaaaaa tacgagctga actcgatttt gcgaatgatc tttatgaaaa attgggctgc
    13921 gtagtaatca atgtcgcttc tttatctatc gaagaaacag cttcacttat tttgaatgct
    13981 ttagacctag aagatcatag ttattatgga actgaaacca ccgaagaaac agactaacag
    14041 tttttcttgt ctttttctta aaaaaactgg actgtgtcag cgaagaagat tcgttgatac
    14101 agtccagttt gtctctattt gcacctatcg ttattgatgt tccacatgtt tgagcgtctg
    14161 gttcaaaata tccagtacgt gagaatcatc aagactatac aaaatcgctt ttccctcacg
    14221 atgggacttg acgacatggt tttctcttaa taaacgcagt tgatgcgata cagcagattg
    14281 ttccatttct actgcttggg tgattgctgt cacattttgt tccccgtctt tcagatacag
    14341 cagaattttg agacgagtgg ggtcactcaa cattttgaat agttggctga ctttctctac
    14401 ttttgttttt tgcgtgtctg gcaatccatt cacctccaac ttgaaaaaag actgacttct
    14461 aaagtttacc actttttcca tcagtctcaa gtttttttat tcttctgtca tttttttgat
    14521 aatcgcttca taagcggcta ctttttcagc tgcgtcttct gaagagtttc cttttacacc
    14581 gatataaaat ttgattttcg gctcagttcc tgaaggacgg atagcgatcc agccattttc
    14641 ttccagcgta tacttcaata cgtcagatgg cggtgtcgtt agtttttcta cgtttccttc
    14701 acggtctgtt ctcgtcaact ctttgaagtc ttctgtttgg atgacttttg ctccggcaaa
    14761 ttctgtcggt gcttcttcac ggaatttttc catcaaagca gagatttttg cagaaccttc
    14821 ttgtccgctc attgtcacag aaatcgtttt ttctgcgtag tagccatatg tttcaaaaat
    14881 cgtttgcaag ccgtcataca acgttttgcc ttgtttttta tagtaagctg ctacttctgc
    14941 taataagagc aatgcttgaa tcgcgtcttt gtcgcgaacg aaaggtttaa ctaaataacc
    15001 gtaactttct tcgaatccga acataaaagt atgggaatga tcttcttcaa attgttgaat
    15061 tttttcagcg atgaatttga atcctgtcaa tacattgaac atggtagccc catagtgttc
    15121 cgcaattgct gtaggcaatt cgctagaaac gattgatttc aataccgctg cattttcagg
    15181 taaagtgcct cgcgattggt gtgcttccaa aatatattga gcaaacaggg ctccgatttg
    15241 gttccctgtc aatacttcat aggagccatt tggcaaacga acggctgcgc ctaaacggtc
    15301 tgcatcagga tctgttgcaa tcagtaagtc agcgccttct ttctcgccaa gtcgcatcgc
    15361 atactcgaaa gcagagtgtt cttctggatt cggtgatttg actgtagtga aatccgggtc
    15421 tgcgacagct tgttctggaa ctagtacgaa ttgttcgaat ccagcttgtt tcaacgcacg
    15481 ttcgcccaac atttttcctg ttccgtgaag tggtgtgtac acaagtttca aatctttacc
    15541 cattgtttcc accaattcat gatcaatcgt tactgctttg atttcttcta gataagcggc
    15601 atccacttct tctccgatga tcgtgatcaa tccgctatgt tcagcttcgt cgtcagataa
    15661 tacttcaact tttaacggat tgctgacttc acggacatat ttcgtcaatg catctgcgtc
    15721 tgcaggaggc atttgtcccc catcttctcc gtagacttta tatccgttgt aagctgctgg
    15781 attgtgggat gcagttacca tgattcccgc aaacgtcttc aaatgacgga ctgcaaaaga
    15841 aagctctggt gttggacgta aactttcgaa tacataagaa gggatattgt gatgtgctaa
    15901 tgttttggct gcttccatgg caaattctgg tgattgatgg cgtgagtcat aagcgatagc
    15961 gacacctcgg cgttttgttt cttcaccttg tgtatccatg aaacgtgcaa gtccttctgt
    16021 tgcttgtctt accgtataga tgttcattcg attgatccca ggtcctaaga ttccccgcat
    16081 gcctgcagta ccgaattcca acggtgcata aaaagcatct tcaagttttt caggatcttc
    16141 acccagatca attaattgtt tttttaagtt ttcttctaaa ttttcttcat gtacccattg
    16201 ttcatacact tgttcccaag acatgaaaat aacacctctt tctgaaatat ggttcttaat
    16261 aagtatagca ttcacttatt agatatgaaa gtacgaggtc taaaaaacgc ttaaatcaag
    16321 aaaggattct tttatcaagc acggttgcaa gtattcgcat aaatcggatt cagtagattt
    16381 cacgcgaaaa aaaagagtaa aagaacaatt gaaaccgttc ttccactctt tttaaactta
    16441 tgcagttgtt tcttctaaag attccaagta tttatacact tgttgtccag cgatcccacc
    16501 atcaccaaca gcagtagtga tttgtcgcaa atctttttca cgaacatctc cgatggcaaa
    16561 aacgcctggt atattcgtct tcatttcgtt gtctgttgga atccagccag cttcgttcgt
    16621 caaccctgct tctctaaatg gtgcagttaa tggatccagt ccaacataga tgaatactcc
    16681 gtctgctgcc aaatcaaact cattgccggt tttgacatta cgtgctttca cacctgtcac
    16741 taccatttca ttcccaacga tttcgtcaac gactgtatcc cacataaagg aaattttttc
    16801 attcgcaaat gcacggtctt ggatgatctt ttgtgcacgt aattcatctc tacggtggac
    16861 gatgactact tcagaagcaa attgtgtcaa atagatggct tcttctactg ctgagtctcc
    16921 tcctccgatc actactagac gtttgttacg gaaaaacgca ccatcacaaa ctgcacagta
    16981 agaaacgcca cgtccagcaa attcttcctc gccaggtacg cctaatttac ggtggataca
    17041 gcctgttgcg ataatgactg tttttccttc gtaagtttta tcttcacaaa taacttcttt
    17101 gtggctgccg tgatccttga tatcttgcac gattccataa gcattttctg tcccaaatct
    17161 tgaaacacct tcatacatct tataagctag atcaggaccc atgattgaat cgaaaccagg
    17221 atagttttcg atttctgccg tattgttcat ttggccgcca ggggcgcctt gttcgatcat
    17281 caatacggat aagtttgaac gtgaagcata taaagcagca gtcatcccag caggacctgc
    17341 gccaataacg attacatcat acatatgtag attctccttt acttaatcaa gtaagcatac
    17401 tttacagcca atgcgaaaaa aagtctattg ttctgcttgc gtttctcgaa tagctttatt
    17461 atactcgaaa aatccgctga aaaacaatag acttttacaa atcattgatc aatcattcga
    17521 aagattttct ccatttgaat caattacttt tttataccag ttgaatgatt tcttcttcga
    17581 acgggctaat gtgccatttc cttcgttgtc acgatcgaca taaatgaatc cgtacctttt
    17641 cttcatctca cctgtaccag ctgatactaa atcaatacat ccccaagtcg tatagcctaa
    17701 tagatcgact ccatctaatt ctactgtatc tttcattgcc tggatatgtg ctgccagata
    17761 gtcgattcga tagtcatctt ccacataacc attctcatcg ggcgtatcga ctgcacctaa
    17821 gccgttttct acaataaata gtggtttttg ataacgatca taaatatcat tcatcgttat
    17881 tctcaagcct aatggatcaa tttgccaacc ccattcactt gcttccagat atggattttt
    17941 gacagaagca aagatatttc ctgcagtctg ttccaagagt tcgttgtcag tagtcgaaac
    18001 tcgagaagaa taataagaaa aggagataaa atcaacagta tgttctttca gcaatgcggc
    18061 atccccaggt tccattttga tctgaatgcc atttcgttcc atttctttca atgcataagc
    18121 gggatattct cctcgtgact ggacatcaat gaagaaataa ttttctcgat ctgattttct
    18181 agaagcccaa acatcttcag gattgcaagt gtatggataa tatgctcctg ctgccagcat
    18241 acagcctact ttgttctccg gatctacttc atgagcaatc ttcgttgcga tggcacttgc
    18301 aaccaactca tgatgtgccg cttgatattt gatttgttct tcattttctc cttcttcaaa
    18361 atacagccct gcgcccatga atggtgcatg aaggatcata ttgatctcat tgaatgtcaa
    18421 ccaatatttg acaagtcctt tatagcgatt gaagatgact cggcacagat tttcataaaa
    18481 accaacgagt tctctagaac gccacgcacc atattctttg atcaaatgca tcggacaatc
    18541 aaaatgtgta atcgtcacaa gcggctcgat cccgtacttt tgacattctt taaagacatc
    18601 ctcataaaat ttcaaaccag attcatttgg ctcattttca tcgcccattg gaaaaatacg
    18661 actccaagca atggacatcc gataagtctt gaaccccatt tctgcaaaca aagcaatatc
    18721 ttccttataa tgatgataca tatcgatcgc aatttttgct ggataaaaat gatctttatc
    18781 aaattcaaac attttctttt ttccagtaat gacaggaaaa cggtcttctc cgattggcac
    18841 aacatctacg ttcgctaacc cacgtccgcc ttcatcataa ccaccctcgc attgattggc
    18901 tgctgtagca ccgccccata aaaaatcttt tctgaatccc atcatacttc ctccattaca
    18961 tgtgtattgc ttgacaattc tcttgccagt tcatcttgga cttcgattcc ttctgctttt
    19021 aaccggctga ttgtttcctt gaattgcggt aaatactgct gatgtgcaat aaacaactca
    19081 tccatgatcc gttttgctgt ctttcctgat cgtataagcg gattgatcgt aaacgcttgt
    19141 aaagctgttc cataatcacc agtcacggct gcttcgatcg tcagcaattc catattcttc
    19201 attacttgca accagcctct ttcagcagca ggaagggaac caaacgctac atttcgtgcg
    19261 ccttgtgcgc caacataggc agtgacttct actacacaat ctgccggcag atcaggaaca
    19321 gctccatcat tctttgtaga caccacgatc tgtgtttctt tatttccata gatcgaagca
    19381 atcgtttcac aggcagcatc tgaataataa gcaccacctc gtttttgcaa ttgttcaggt
    19441 ttatgatcca attctggatc tttgtataat tcaaacagct ctgcttctgt ttctttcact
    19501 tgttgcgctc ttgtaccaat cgtttgatat tcttctaaag catgttccaa catttcttct
    19561 tcacggtagt aatagcggtg gtagccacaa gggatcatgt tcatttgttt taattgttct
    19621 ttgaaaaacg ggatgtcaaa gatatttttc ggcaatccat tgtcttcatc gtataaacga
    19681 tcaatcaaat ctgctgtgcg gtcatttcct ttattatctg ctactttgtg ccaatggaag
    19741 tgatttagac cggcaaactt gtagatcagc tcttcttttt tcttgccaag cattgctggt
    19801 tctgtcatcg tcgccattac tggcacattg cataatccga tcactttatc ccatttgcca
    19861 tatctgataa ctgcttctgt caccatcccg cttgggttcg cgaaattgat cagccaagca
    19921 tttggacata aacgcttcat gtcttcaacg attgccaaaa ccactggaac ggtacgaaaa
    19981 gctttgaata tcccgccagc accatttgtt tcttgcccaa tcatcccata ataactaggg
    20041 atccgctcgt ctttgattcg tgcatctaat tgaccgacac gaaactgagt cgtaacaaaa
    20101 tccgcatctt tcaatgcttc ttcccgatcc aaagttgcat agatcttgac ttcatacgga
    20161 gaagcatccc acatacgctg agacatctga ctgacgatct caagcttttc ttttccttct
    20221 tctacatcta cgagccagat ttctcgtatc ggtaattcct catatctttt gataaatcct
    20281 tccatcaact cgggggtgta actgctgcct ccaccaatcg tagcgatttt gattcctttt
    20341 gccatgttct cactcctttg ctttttacac ttttattatc ttttttgtaa acaaaacaaa
    20401 caatagaaat tcgtttcttt ccagtaacat ttacaaaagt tagtgttttc tactggattt
    20461 ttgtaaatat cttgtcaaaa aatgacttta ttaagctata taacataaat ccgtttacaa
    20521 atcattttca aaaaagttat aatttacgta aaaaaactag aagaaggtat ctacatgtta
    20581 ttacgtgaaa aaatcaaaga aatctccttt tctccttcag aaaatgaagt gattcagttt
    20641 ctcttaagcg attctccagc acttggcgaa atgaccatcc aagagattgc tgagcgatgt
    20701 tatgtccacc cgtccacact catccgtgta gcaaaaaaaa tgggttttga tggctgggtc
    20761 agttttcgta atgctttttt agctgaaaca gcctatcttg aagggaattt tcaagacgta
    20821 gatgcgaatc ttcctttttc caccacagac ggtattatga cgatcgcaaa taaaatggct
    20881 tctttagaac agatgacgat tcaagataca ctttctctca ttcatcatga ccaactgcaa
    20941 aaagccaaac aactaatgct tcaagcaaaa aaaatcgtga ttttcgccag taactcgaat
    21001 actttgattt ctcaagattt catgctgaaa atgaagcgca tcaaaatgga tgtcacgatt
    21061 gtcaccactt ttggtgaatc gttctatgaa gcgtacaatt gcacgagtga tatgtgcggg
    21121 attctgattt cttatacggg tgaaaacaat gtgatccttc gtaccgcaga aatattgaaa
    21181 caaaatcatg ttccaatttt agcgttgacc agtatcggtg attcaacgct atcctcaatg
    21241 agtacagccg ctttgcgact tacgacacgc gaacgactgt attcaaaaat tggtaatttt
    21301 acgatcaata catcgatttg ttatcttcta gacgtgttat atagttgtgt ttttgcagaa
    21361 aattatcaac agaatttagt ccatctaatt tctgtaggac aaaaatttga taaacgccct
    21421 atcagctcag ccatcatgaa agagcctttc aattttgaaa ataaagaagt ggactaaaaa
    21481 gaatagttaa gtctattcca ttctttcacc taaccgttca ggtcagtgcg gtgataagtg
    21541 catcgtctgg cccgaccgtc tccccttgat tttccaaaat atccaaataa tccatgtaat
    21601 tggtgacgat tacaggtgtt tctaaactgt atccttcctt ttcgattttc tttttgtcga
    21661 agcggagcaa cagatcgcct tgtttcactc tttctccttg ctgcaccaag ctttcaaaat
    21721 atttcccttc caactgaaca gtatccaatc cgacatgaat cagcaattcc agacctgttt
    21781 cagaaaccat tccgattgca tgtttcgtgg ggaacagggt catgactgtt ccgtcaaaag
    21841 gggcacgtac ttctcctttt tcagggtaga tcaagattcc tcgacctagt gttccttgtg
    21901 caaaagctgc atcctttgct gctttcagtg gagcgatttg tccttgtatc ggtgttttta
    21961 caatttcttt acctgatctt ttctgtctgt catctgtttc acctgcttcc tccttccaaa
    22021 agaaaaaagt cagcgcaaag gataccacca tcgcaatggc gatcccaatc aatacattaa
    22081 tcacatctgt taaatcacca tctggtctaa tatagttggg aattgtaaag atgcctagtc
    22141 caccttgatt ccaagaagtt aaattcatta acccactata cgcgccccct aacgcaccac
    22201 cgacacaagt aaaaataaat atttttttac gtggtaatgt aaacccataa atagctggct
    22261 ctgtaatccc cacaatacca gaaacaatgg ctggtattgc aattgctttg tcttttggat
    22321 ttctaagctt aaagtagagt gcggtaagtg ctgcagtttg tccaaaagac actactgcaa
    22381 caggtgtcat aatattttgc caaccattca cagaaaattg catcaatccc attggtacga
    22441 ttgcccaatg gagaccaaac atcaccagaa tttgccagaa gaagcctacg attgctccat
    22501 aaataactgg cgaaagtgct aaaagtgttt caaatccagc cattaaaata tcagttgcga
    22561 atgtgaatac tggtccaacg agtaaaaaac ctaaaggcat tgtaataaga acggtgaaga
    22621 atggaacgaa gaaattcgcg atcatctcag gtactaactt tttaattggt ttctgaattt
    22681 ggctagctaa caacacaata aagataatgg gaatgacaga agaagcgtag ttacgcgcaa
    22741 tccagggaat gccgaatact gtttgataaa taggtgcttc aaaaatagtg ccagaaaata
    22801 tggtcgttag tggtgcttct gctgcaccac tcaaagcttc cagcgacaag ctaggattca
    22861 ttaatgctgc gccaagcaac atccctacga attcatttac tttaaattta cgggcagctg
    22921 ttaaaccgat aataacaggc ataaattgga atacagaatc accgatatta tcaaacataa
    22981 caaatgtgcc agaaccgcgg tcaaacgcgc ctaagaagac aaggaatgct gtcagtccac
    23041 gaatcatccc gccagcagct aagatagcaa ggaatggttg gaaacagccc gatattccat
    23101 caataagcat atcaaacggt ctcatttttt ctgttgaaac agcctcatca aaagtaaggt
    23161 ttcctgcttt tataacctct tcaaacacga ctggcacatg attcccgatg acaacttgat
    23221 actgccctcc cgctcgcatt accgttacaa taccgtccat atttttcaag atctcatcat
    23281 ttgctttttc ttcgtttttt aatttgaaac gaagccttgt gatacaatgt gtcaaaccat
    23341 tgatattttc cttcccaccg acattttcta cgatttttct cgctaattct tggtattttc
    23401 ccatttctat tccctccaat atattagtgg aaggtttagc caactgaatg tcactaccct
    23461 tatttcatac gttcttgtta atttatcctc ctttttttat ttatttacaa cgattctcat
    23521 gcactaaacg cgcagtatgg atcgttaaat acattttttc gtccgtggac atctggtagt
    23581 tgaacgtctc ggctaaaaag tctgctattt tctcaacgca accaaacgta tccttatatt
    23641 ttttttgaat aacctgaaac aattcctcag aaacttctcc cacttctgat ttttctccat
    23701 ctaggacgcg tgtcagaaaa aaacgtaagt gtgtgacgaa tcgaaaaaaa tggactgaat
    23761 cttcatcaaa tgtaatctta tagtaatagc ggacgatatt tagaatatcc tgtattgtta
    23821 tagtaaacag gtaggtattg ttttgttggc tttgaatgcc ttgtgcattg actaaatgca
    23881 atgcgataaa tccggcttca tcttctgata acttgatgtg gtatttatcc gagattttct
    23941 tcactgtttt tttcccgatg gcatactctt taggaaatag ttgtttgata tcatacagca
    24001 atacatttgg tacaaatatt cccattttgt gacgttcgat agccgtatac aaatgatccg
    24061 tcaatgcaat atacacagaa tcattcaact ggacttgtag agcctctttt gtatattcaa
    24121 tgatttcaca agaaatttct aagtactcaa gaggaatcgc ttgtgccaat tcttggaact
    24181 gttgtgaaag cccgtcattt gtcaactgaa attttttgtc gatgcatgag tcaaaaatcg
    24241 ggtcacccac tttctttttg aatgccaaac cttttcccat cacgatcatt tcctgatttc
    24301 tttcatccct ggttattacg acgttgttat tcagtacttt ctcaatcagc atgttttgct
    24361 cctttccatg atatataggg ctgtattcaa aacaaaaaga aaaacccaaa aagccgtcca
    24421 tggaaccatg ggagactcat caggtttagc ttgcatcagg caatcactat ccaagttgta
    24481 tcgttttctt tatattatcg aaagcaaacg cttttgtcaa cgctttctta gtggctatta
    24541 ggcacttttg actagctgtc gtcgtctttt ttaaatcggg tgactacgcc actaacgctc
    24601 acagatgaag aatctgggaa gctctgtttt attttcactc tagcgcttag gacaaaatgc
    24661 tactatgcta ctgtcacaac ctctttttac acaccactgc tttgcagttt gtacatatct
    24721 gcatacaaac cattttgcgc tagaagtgtt tcatgattcc cacgctctac tatatgacct
    24781 ttgtctaaaa ctaaaatcaa ttctgcatcc cgaatcgtgg acagccgatg ggcaatcgca
    24841 atcgttgtcc gtccttgtcg catttttgcc aatccttctt ggatcaatcc ttcggtttca
    24901 gtatcgatat ttgctgttgc ttcatccaat actagtattt taggatctgt cacgattgtt
    24961 cgcgcaaaag tgatcagctg tcgttgtcca ctagaatagc tggcaccgtg ttcgatcact
    25021 tttgagtgat atccttttgg taattcatcg atgaacttgt ttgcttgaac aaactcagct
    25081 gcatggcgaa tctgttcgtc tgtgatagct ggattcatca tccggatatt tcctgcaata
    25141 tctccataaa acaagaaggc atcttgcaag accaatccca ttttcttacg taactcttcc
    25201 attggatatt ctcgaatgtc atgatcatca atcaagattt ggccttcata aaattcgtaa
    25261 aaccgcatca atacgttgat gatcgagctt tttccgcttc cggtgtgccc cactaatgca
    25321 acggtctcac ccggattagc gacaaaagag atattgttca atacattgtg ctctccatca
    25381 taagaaaagc ttacattgcg aaactcgatc ttacctgaag taatcgcttg atctgctcca
    25441 ggattttgtt gcggtgcata ttcttctgta tccatgattt tcaaaatacg actaccggca
    25501 accatcccat ctgtaaaggt gctcaaaaag tccatcattt gagacattgg attaaaaaat
    25561 ccctggacat aggtcgtaaa tgcatagatc agtccaactt ctacgggaga atgcaacgca
    25621 tcgatcccaa acaaagtcaa cgccaacgca atcgccaatg catagagaaa attgatgatc
    25681 ggacttaaca gaatcgaatt cgttttgatc atcgcatagc gcgtgtctaa atactcgttg
    25741 ttcacttgtt caaattcttt agctaaacga gcttcttggc gaaactgctg gataatacgc
    25801 atgccggaaa tcgattcatt caatttcgtg ttcagctgac ttaatttttc ccgcatattt
    25861 cggtaaatac gagaactgaa tttctggtaa taccagatga cgattcctaa aatcggcaag
    25921 aacaccaaag tccacaaggc aatttctcca ttgatttgaa acatggcaac gaaagaagaa
    25981 acgactgcaa agatcccagt caataccatc aaaaagacat accaaaattc aaatagcgtt
    26041 tctgtgtcat tcgtcactcg agaaacggtc gatcctgccg gtgtctggtc aaaataacgc
    26101 atacctagtg tatgcagctt ttcaaataat cttacccgaa catactgata ggtcttcaat
    26161 gaagccatgg aatacaaata ccattggaaa aaccaaatga tacttttgac tatgactcca
    26221 aacaagtaca caccagcaaa aaacaaaata acttgtgtcg tagctgactg tttagccaga
    26281 tgatcatcca taaaagtttg gatgatcctt ggcaatagga cattgatcac agacagggca
    26341 aacgcaaata agatagcaac gatgaacgtt cttctaaaag gtttggcgaa cttcatcagt
    26401 cgtttgatga tcttcttctg ttctttaagt ggaatcgatt ttgtccattc cgattgataa
    26461 ttttcatcca tcaggcctcg cctccttcta tcttcgcttc taattgctgt ttttcccaca
    26521 ttcgttcata ccagccacct aatgcaagca gctcgtggtg ggttcctcgt tcaatgattt
    26581 tcccttgatc gacaacaatg atttcttttg catgcatcac gctgcttaaa cggtgagctg
    26641 caatgatggt tgttttctct tttcttattt cttttaaatt atgcaagata gcttcttctg
    26701 ttttggcatc tactgcagaa agtgcatcat ctaagatcag caattcagga tttttgatca
    26761 atgctcgagc aatcgaaatc ctttgtttct gtccgccaga taaagagact ccacgttctc
    26821 caactaaggt atcataacct tttggcaatt ccttgatttc attgttgata aatgctaaag
    26881 aagccgcatg ctctacttct tcttctgaaa gttttggatt agaaaaacgg atattatctc
    26941 gaatggtcat cgaaaacagg aagtgatcct gaggcacata accgattgat cctaataaag
    27001 cattcaatga atattctttg atcgaacgtc cgccaaattg gattttccct tgataatcat
    27061 cgtattcacg catcagtaat ttgatgattg tcgtttttcc cgcaccagtt ttcccaacaa
    27121 tacctaatgt ttctccttct tggatcgcaa atttgatttg ttctaatgct ggtgattctt
    27181 ctttcggata gttaaaagag gaaatagcta cattcagctc gccttttgcc ggggtctgca
    27241 ctgcacccag ttgttccacg atatgcgtct tttcatgcaa aagttcatta acgcgatcat
    27301 aagaagcatt tcctctttcc aatacgttga acaatcggcc aatcgcaaac atcggccaaa
    27361 caagcatacc gatataacta ataaatgaga ccatctgccc aatcgagatc gtgccttcca
    27421 agacatactt tccacctaaa ataattgttg caacataaga cagaccgata atcaaggtaa
    27481 tgaatggatc aaataaggag tctaaaaaat tgactcgttt gtttttaata attgcatctt
    27541 caatcttttc ttcaaaatcc gcgagatctt ctttttcttg tccaaatgtt ttaatgactt
    27601 tgatcccagt gatactttct tgtgtcttat cattgatact agaaaaagct tcttgcgaat
    27661 cacgaaaagc atcgtggagt tttgacccca gcactcgtga agtaacggct agcaaaggca
    27721 gaggaatcag cgcaatcagt gttaagcgcc aatcaataaa caaaatcatc gcaatgatcg
    27781 ttgttccgcc cgtaatcact gaatctgcaa aggtcaagat tccagctcct gctacattct
    27841 ggattgcatt caaatcattc gttgcatgag ccatcaaatc tccagtccga tatttttggt
    27901 aaaagagatt gtccatttct gtaaaatgtt tgaatagctg ctgccgaagt tctttctcca
    27961 accgagctgc acttccccaa atattcgtcc gccagatata acgaaaaaga tattgtaaga
    28021 aagctgcaag gatcaatagt aagatccacc aagaaatgat ctttattgat aaattcttat
    28081 cggcgatctc atcaatgatg atcccaatca cttttggagg aactaactgt accaaggcca
    28141 ctgcgaatag ggagaaaatc ccaattaaat aatgtttctt ttcctgacga aaaaaccaac
    28201 ctaattttct aaaaatcgac attttactcc tcctttgtct ttatcaagag cataggattg
    28261 tctgtccttg tcttttttgc tgaatagtct gaatagtcta acaaattacg aaataaaaaa
    28321 cggactgaaa aaatcagccc gcaaaaagtc tttgggtcag aacctatttt ttcccttggt
    28381 tcttcatttg ctgcatcatc tggcgaattt tcttctcaga tggtttttgc cccattgaca
    28441 tcatcattga tcgcaacatg tcctcattaa cgggaggatt tttttctaaa tagtctttca
    28501 tatatttacg cgctaggaaa aagccaccta ctgcgccgat tagcaaagca ataatagcaa
    28561 taagtacgac aatacctgtt gataccatat aaatctctcc tttctatttc cgaattgctc
    28621 tcaatgattt attttactaa agtttattga aaaaagaaag gcgtattctt tagtttcatg
    28681 caaaaaatag tggttttttt agagaagaac aaaatttctt tcattaacca gcaagacacg
    28741 ccacgaaaaa aaattaggca taccgaatct ttttctttaa ttttttcttt ctgaggtgta
    28801 gaatgtaatc gtagataaat aggaaaggtg gttttttctt gaataatcaa tccaacaaac
    28861 aatttcataa gcatcgactt attgaacata caaatggttt atctttagct gaagtaaatg
    28921 gaacagtgga tgtaccaaag ggaaaaagtt tttttaaaac acttcttgct tactctggac
    28981 caggtgcatt agtagcagtt ggttatatgg atccaggtaa ctggtccact tcgatcactg
    29041 gtggacaaaa tttccaatat ttattgatgt cagttatttt gatgtctagt ctaattgcta
    29101 tgcttttaca gtatatggca gcaaaattag gaatcgtgac ccaaatggat ttggcccaag
    29161 caatccgagc acggacaagt aagacattgg gtgtcatttt atggatcttg actgaattag
    29221 ccattatggc aacagacatc gcagaagtca ttggtgcagc aatcgccctc tacttacttt
    29281 ttgatattcc attggtaatt gccgtattta tcactgtgtt agatgttttc gtccttttat
    29341 tgctgacaaa agtcgggttt cgtaaaatcg aagctttggt tgtctgcttg attttcgtca
    29401 ttttattcgt tttcgtctat caagttggct tgtctaaccc tgattggcac agtttatttg
    29461 ctggctttat ccctaacagt catacattct cagactcacc tgtcataggt ggtgaaactc
    29521 cgctgactgg agcgttaggg attattggtg caacagtcat gccgcataac ttatatcttc
    29581 attcagcagt ttctcaaact cggaagatcg atcaccaaga cgaagaagat gtagcacaag
    29641 cggtacgctt ttctacttgg gactctaata tccagttgtc aatggctttt ttagtcaatg
    29701 cactgctatt gatcatgggt gtcgctgtat tcaaaaccgg ttctgtccaa gatccttcct
    29761 tttttggtct gtatgaagct ttatctgatc catcagctat gagcaatggt atcttgattg
    29821 ctgtagcaaa atctggtatc ttgtctactt tatttgctgt agcacttctt gcttctggcc
    29881 agaactctac tattactggt acgttgaccg gtcaagtcat catggaagga ttcatccata
    29941 tgcgtatccc gatttggcta agaagactag tcacacgatt gctttctgtg attcctgtat
    30001 tgatttgtgt tttgtttaca agtacaaaag gaacgatcga agaacatacc gctttgaata
    30061 acttgatgaa tgaatcccaa gtttttctgg cttttgcatt gccattctcg atgattcctt
    30121 tactgatgat gaccaatagc caagcggaga tggggagccg attcaaaaat tcatttgttg
    30181 taaaacttct tggctggttc tctgtgatta gtctgactta cttgaactta cgaggattac
    30241 caggtcaaat cgaaagtttc ttcggcgagc aagccacctc agctcaacta gcattggctg
    30301 atcaaatcgc ctatgtaatc attgttcttg tccttctcct gttggcatgg acgattggtg
    30361 atttatataa aggaaacaaa aaatatgaac agcgtttgca agaaacgata gaagaaatcg
    30421 aatagaaact gattctttta cacaaaggag gttgtgacag aagtgggcag cttcaagaaa
    30481 taagaaggaa tttaagaaaa ttgcttttca aatttttgtg aaatttcggc ttatttccga
    30541 agaagttact tctggaacac cgtgtat
//
LOCUS       DACECB010000028        29694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECB010000028 DACECB010000000
VERSION     DACECB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.028"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1817)
                     /locus_tag="IWO04_001361"
     CDS             complement(<1..1817)
                     /locus_tag="IWO04_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212983.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPV"
     gene            complement(1804..2022)
                     /locus_tag="IWO04_001362"
     CDS             complement(1804..2022)
                     /locus_tag="IWO04_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0212984.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            complement(2098..3111)
                     /locus_tag="IWO04_001363"
     CDS             complement(2098..3111)
                     /locus_tag="IWO04_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0212985.1"
                     /translation="MEEIDKLIAQYAYSKDDVYAEKYLKYPIFLDKHKKYKKMKQSTK
                     KVYMYLKKQNEYSLENSKVDSKGRIFLIFAIEKIAEKSCLTKKTVISALRELEEYKLI
                     TIKKNGFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKNTPS
                     DKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIES
                     LIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEI
                     CNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGI
                     DKQ"
     gene            complement(3315..3596)
                     /locus_tag="IWO04_001364"
     CDS             complement(3315..3596)
                     /locus_tag="IWO04_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0212986.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(3647..3853)
                     /locus_tag="IWO04_001365"
     CDS             complement(3647..3853)
                     /locus_tag="IWO04_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0212987.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            4017..4748
                     /locus_tag="IWO04_001366"
     CDS             4017..4748
                     /locus_tag="IWO04_001366"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0212988.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            4802..5947
                     /locus_tag="IWO04_001367"
     CDS             4802..5947
                     /locus_tag="IWO04_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0212989.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(6032..6337)
                     /locus_tag="IWO04_001368"
     CDS             complement(6032..6337)
                     /locus_tag="IWO04_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0212990.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6362..6793)
                     /gene="ruvX"
                     /locus_tag="IWO04_001369"
     CDS             complement(6362..6793)
                     /gene="ruvX"
                     /locus_tag="IWO04_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0212991.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6790..7056)
                     /locus_tag="IWO04_001370"
     CDS             complement(6790..7056)
                     /locus_tag="IWO04_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0212992.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7311..8036)
                     /locus_tag="IWO04_001371"
     CDS             complement(7311..8036)
                     /locus_tag="IWO04_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0212993.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8397..8467)
                     /locus_tag="IWO04_001372"
     tRNA            complement(8397..8467)
                     /locus_tag="IWO04_001372"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Gly,seq:ccc)
     gene            8584..9336
                     /locus_tag="IWO04_001373"
     CDS             8584..9336
                     /locus_tag="IWO04_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0212994.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9352..9831
                     /locus_tag="IWO04_001374"
     CDS             9352..9831
                     /locus_tag="IWO04_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0212995.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10082..11662)
                     /locus_tag="IWO04_001375"
     CDS             complement(10082..11662)
                     /locus_tag="IWO04_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0212996.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11655..12398)
                     /locus_tag="IWO04_001376"
     CDS             complement(11655..12398)
                     /locus_tag="IWO04_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0212997.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12574..13350)
                     /locus_tag="IWO04_001377"
     CDS             complement(12574..13350)
                     /locus_tag="IWO04_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0212998.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13598..14434)
                     /locus_tag="IWO04_001378"
     CDS             complement(13598..14434)
                     /locus_tag="IWO04_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0212999.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14445..15374)
                     /locus_tag="IWO04_001379"
     CDS             complement(14445..15374)
                     /locus_tag="IWO04_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0213000.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15566..15919
                     /locus_tag="IWO04_001380"
     CDS             15566..15919
                     /locus_tag="IWO04_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0213001.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15993..16649)
                     /locus_tag="IWO04_001381"
     CDS             complement(15993..16649)
                     /locus_tag="IWO04_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0213002.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16875..18809
                     /locus_tag="IWO04_001382"
     CDS             16875..18809
                     /locus_tag="IWO04_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0213003.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18895..20118)
                     /locus_tag="IWO04_001383"
     CDS             complement(18895..20118)
                     /locus_tag="IWO04_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0213004.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20112..22010)
                     /locus_tag="IWO04_001384"
     CDS             complement(20112..22010)
                     /locus_tag="IWO04_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0213005.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22004..23122)
                     /locus_tag="IWO04_001385"
     CDS             complement(22004..23122)
                     /locus_tag="IWO04_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0213006.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23205..24038)
                     /locus_tag="IWO04_001386"
     CDS             complement(23205..24038)
                     /locus_tag="IWO04_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213007.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24031..24948)
                     /locus_tag="IWO04_001387"
     CDS             complement(24031..24948)
                     /locus_tag="IWO04_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213008.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25175..26299)
                     /locus_tag="IWO04_001388"
     CDS             complement(25175..26299)
                     /locus_tag="IWO04_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0213009.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26482..27390
                     /locus_tag="IWO04_001389"
     CDS             26482..27390
                     /locus_tag="IWO04_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213010.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27531..29144
                     /locus_tag="IWO04_001390"
     CDS             27531..29144
                     /locus_tag="IWO04_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0213011.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9388 a         5742 c         4766 g         9798 t
ORIGIN      
        1 cttacaggat tattattcga ttcagcatta tttttcattg cttcacgcat acgttcagaa
       61 ctagacataa tactatcggc attttcaacc ttagcaccat cttttctatc cgaaagacgt
      121 gttggcatgg aatcagcata ttttccagtt tcatcttctt tatttttagc taacataggt
      181 ttgctatttt tattaatagc ggacacagta ccatcatcta aaccttcttt aaacttactc
      241 ttggtatcat tagctatatc acgcatactc ttaaatggtt ttgttgctct ttcactagcc
      301 attgtagatc catctgaagc taaacctgat aatccacgtt catgagcata cccaagcgct
      361 ttccccgttt tattagcttt tttagaaagt gattgtccaa agttgcgttc aggttttcgt
      421 tcttcccctt cagcttgatc gtttccacca ttatttttcc cacctttaga ccggttcata
      481 ccacctgttc ctttacggaa tagcattgca cccattccaa aagcagaagc aagttgccca
      541 tagccttctt tgagtccaat atccacacca aagtatctca tgactgttcc actgcctttg
      601 attaaaacaa acacagcaca gatataagca attgttttta taaaaatatt tgctgagata
      661 ccttcgccag tatttaggta atttatgaac attgcaaaca tagataaacc gaacccttga
      721 aatccgactg tcaaatagca ctgtaaaata tctgacaaaa cttgttttga tcgttgtcct
      781 gtttctaagt cagtagcaaa tactaaaact ccgagtacac gtttaaatgc taactcgagt
      841 attgctgtaa ttataatgaa agcactaaaa acatacgcta ccgctaaagc aatcaatcca
      901 aaagaaattc cccacaggtt cgcttgatag cgataatatc ctgatttgat accatcaaca
      961 aaggttgata aaaaattatc tgaaaattta gtagcaacaa attcattatc agaattttca
     1021 actaattcat aacgcagatt atcagctgtt gggttctctt tttccatttt atcaattaca
     1081 ttatctgtca atacttgcgt aagatccgtt ttccagaaat tttcttttgt tagattattt
     1141 cggtcatttg ttttatcaat tgtcgaatat tgatctgtct tattaatata agctaaatct
     1201 gtcactccct gctcaatcaa tgaccatgct acaccgccat cttgattatt gatactagtt
     1261 gcgtcattat aaagagtctt tgcaaacatt attcctgaaa gtttttctgt tgatccaatc
     1321 aaagtcggca ttaaaagaat taatgctaca gacatcacta cgttcattcc aaccgatttt
     1381 aaatccactg ttccttttcc agtaatcatt ttgtaaccaa caaacatcag tgaaagtatc
     1441 attaaaccaa taacaactgt acttaagacc gattgataaa ctttattcat tcccgtactt
     1501 tcaataaagc taaacaaatc taacacttcg ggaatcatat tttgaatacc atctacaatt
     1561 gcaaacatcg attttataac agaccaccct atccacaatc caaactgact gtaaaaattt
     1621 ccgttatatt tttttaaata cgattgatag acaggattat tcaaaaattc agtttgactt
     1681 tccgtgtcat caaaccaatt gaaaatgctt gcttggacaa cattaccggt gaaaacaacg
     1741 ataaacatta aaacaagtat gctcaaagct aaccagtgta tttttctact ggcaatcttt
     1801 tctttaacta ttttcattat tacctccctc agcccatttt ctgagactct cagagagctt
     1861 ctgagctgtt tttctgtcaa ttatgatatg agttacacaa ttgtcatttt cagatcttaa
     1921 atcgagaact atagtgttga agcaaatagt ctcgattaat tcgagattat ctgtattttg
     1981 atctagacac caaacttgat ctattaaacg attttcaatc aaaataaccc ctcctatatt
     2041 caaagttctc aaatgaagcg gagatacccc tccacttcat ttgactcaat tctattctta
     2101 ttgcttatct attccttcac tgatttgcaa ttcaattagt tctaacgctt tattttttag
     2161 agaagcaaag atataatcgt ctttttgttt gatacggtga tctgtttttg ctcttcgtat
     2221 caagtcacgt aaagtcattg atagctgttc tctaacataa tctacaaaat acttgttatt
     2281 acagatttca tagataattt tcagttgaat tttttgttct ttttcagcag atttaatcgc
     2341 atatttgaat tttttatacc agtcataaaa ttgactgtaa tcgttattac ccactaattt
     2401 cagacagttt acaatattat ccccccatac ctgataaagc cattcttttt caattaatga
     2461 ttcaattaat atttcatcta attttttatt ttcaactgaa agtgaatcaa taaagtcagc
     2521 attttgagat tctttaaaat ctttaaaatc tttagaagaa tgattaagtt cttgtaaaaa
     2581 tttttcacct ggaagcggga atgacttatc accatcgaat gttgatttgt cggaaggtgt
     2641 atttttttca cttggactgt aatgttgatt tgtcggaagg tgtaaatttt cctcaatgca
     2701 cttttttttg tagaaatctc cttcgctgaa ttctggtttc aataaataat aaaaatttgg
     2761 attattcttc ccagtttttt tgttaaatcc atttttttta atcgtaatta atttatattc
     2821 ttctagctct ctcaaagcgc ttattaccgt tttctttgtt aaacaagatt tttcagctat
     2881 tttttctata gcaaaaatca aaaaaattct tcctttgctg tcaacttttg aattctctaa
     2941 agaatattca ttttgttttt tcaaatacat ataaactttt tttgtacttt gtttcatctt
     3001 cttatatttt ttatgtttat ctaaaaaaat cggatacttc aagtattttt cagcatatac
     3061 gtcatctttt gagtatgcat actgagcaat taatttatct atttcttcca tttattcctc
     3121 ttctttctaa taaatgttaa aatgtagtca aataaagttt tcttagaact ttattgtcga
     3181 gtgtttcctg tggtcgccaa actgtcggga acactccttt taattttttt caaagtattt
     3241 ttatattttc tatacttcat cattattcaa acaaacactt ccttttttct atcaaaagca
     3301 cgccacgcaa ctttttatac ttcatattct ttgataaacc ttgctaattc ttcttttcta
     3361 ataaattgac aatctccgat tatgcttact tttaaacctt tctctctgat aaattttttt
     3421 aatgtacttc tgctacagct taaatactta caagcttccc ccaatctaaa aacttctttg
     3481 cttagagatg tttcaattcg aacttgatct actgcttctt ttgcaactcg atagaaatat
     3541 cttcgcaatt cttgatctaa atccgatgga agaataactt gaatctcaga tttcattttc
     3601 tttgccccca ctaatttgac tacatgtatt caaattcaac ttttcgtcac ttttcattaa
     3661 aaaaaatttg atcaattgtg atattttcaa ttcctttttc tttcacaaga tacttcaaaa
     3721 taatcatttc ttctggagaa tagtttgttt taccgctttc tttgtttctt accgcttgta
     3781 ctgaaatgcc atatttttca gcaagttcag attgtgataa tccacaaaac tttctaaaat
     3841 ctttaggctt catgactcct cactcctcct tcttttcaac ttttcgtcac taaaagaata
     3901 acatatattt attcataccg caacgtaaaa ttcaactttt atctactttt tcatttcatt
     3961 atatgagaat gtagtgtaaa ctgtagaata gaatagatga aggtggtgaa accattttgt
     4021 ttgataaaaa cattaattct gaaattggaa aacgaataca agaaatacgt gtttctttag
     4081 ggtatactct tgaagaattt ggcgaaaatc tgacacctcc cgcaagcaaa agcaatgttt
     4141 ctagatggga aaaaggaaag aacataccca acaaagcgcg gctactaagt attgctaaac
     4201 ttggtggtgt ttctactgaa tatctactga caggtaattc tcaaaaaact aagaaatata
     4261 tcaagtattt attcaatgag attttttttg acaacggtac cttccataac aaattcaaag
     4321 atttcggtaa agacagagat aggcttggtc tatatgactt atttttagca gtatcgaaaa
     4381 catatagtct agataaatca ccttctttaa aagataactt tgtagaaatg attatagatg
     4441 atgtatataa aaaactcttg caaactgata aatcagatga ccaactcatc ctgggtttca
     4501 tatatatttg tcttgatgaa aaactttcaa acagacagaa aagtcttaaa agtatcaatt
     4561 atgaggtatt caattattta ggtgttgcat ttggtttaat tgatagcgaa atcaaatcta
     4621 caaacgcagg taacgatgaa gatttattaa attatttaaa agaattaaca tcttttattg
     4681 atgaaataaa aacaactaca ttcaacaaaa aaaaatattt tcaaaatcaa tatgatatag
     4741 aagaataaag attacatttt cctatcaaaa gcacgccacg caactgatag gaggaaaaac
     4801 aatggcaaca attaaacaat atgaaaagaa agacggaacc aaagcttgga tgtttcaaac
     4861 ataccttgga atcagcccgc taactggtaa agaagtccgt actacacgta gaaattttaa
     4921 aacaaaaaaa gaagctcaaa tagagcttaa tagactactt gtagactttg aagagaatgg
     4981 acttaaaagg gaaacgaacg aaactttcca agaagtgtat gatctctggt atgaaagcta
     5041 taaaactact gtaaaagaag tcactcaatt aaaaattgag atcaagttta ataaatggat
     5101 tcttccaaaa tatgggcagt ttagaattaa agaaattact acaaaacatg ttcagaagat
     5161 attgaatcag tgggctaaaa caacagatca atataaagtc ctacattcta ccgtaagccg
     5221 tattttcaaa tacgcaatga ctctagggct gatcactaaa aatccctgtg agctcgtaat
     5281 catgcccaac agaaatgttg aaaaaaagaa ggatactgtc aaaatttatt cacgagatca
     5341 attagaaacc ttatttcaat atttggaatc caaaagaagt acgtataggg cagagtacga
     5401 taaaacttta ctgcgctttc tattttttag tggctgtcga atcagtgagg ctttagcctt
     5461 agactggtca gatattgact ttgattcaaa caccgtcact ataaacaaaa ccttaagcca
     5521 aacaaaatat gggtacaaaa tttcaagtcc aaaaacgaga aatagctatg gttccctatc
     5581 tttagacaat aaaaccatta attggctaaa gaaatggcaa atcaatcaaa aaagatatat
     5641 gttaaccctc gggattactg atccaacaat gattttttgt ggaatctaca aagaaatcat
     5701 tactaaacac gctatctatt ctcgtatgat cacaatctgc aagaattctg gtattccatt
     5761 tctaggtaac catgtaacca gacacactca tgctagtatg ctgttagagt cgggagtctc
     5821 catgaaggaa gttcaagaac gccttagaca ctcatcaatc aaattaacaa tggataccta
     5881 tgctcatcta actaaagaaa ccaaagaaaa aaccgttgaa aagcttgcag agcacctaaa
     5941 tttctagcgg aacgtattca atcccgtatt caattcaaaa caaaaaaaag ctgaaccctt
     6001 atctaccaag ggttcagcta tcatttacag cttattcttc ttcttccgcc atgaatgtat
     6061 tgaatacttc ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt
     6121 ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg
     6181 aaacaccagc aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg
     6241 ttagtaggat ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt
     6301 gtccttcatg gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt
     6361 attaaaataa ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta
     6421 atttatcgat gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac
     6481 gttctgcctg aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt
     6541 ctttgatctt ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca
     6601 tatttttagg taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca
     6661 accgatcaaa gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag
     6721 ctgtccagcc aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc
     6781 ccattactct cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat
     6841 gatctcatcg cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta
     6901 ggccgggtct ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc
     6961 ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc
     7021 aaaaccaaaa cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag
     7081 gtaattccag cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca
     7141 aatattccaa acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc
     7201 tgtagcttta tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg
     7261 tttttacaca attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt
     7321 gtttttgttg cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata
     7381 aagacattga attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg
     7441 gctaccagtt cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt
     7501 tctttgatat atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa
     7561 cgtgtccctg tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt
     7621 aaatggctgc gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc
     7681 gggtcaatat acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca
     7741 ttggcttgtc ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg
     7801 gaaaggctaa gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga
     7861 tgttttccat catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa
     7921 atagttgtaa tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc
     7981 cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct
     8041 ttactccttt actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta
     8101 gaaagaaata gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa
     8161 agagacggat tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat
     8221 tcaaaaattt tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt
     8281 gcgcaaaagc ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt
     8341 tttgcttggc tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg
     8401 ggtgacggga atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta
     8461 cacccgcaat acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct
     8521 ttacgcttct ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat
     8581 gctatgacgg gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc
     8641 atgatggaaa gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta
     8701 gcttctggaa atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt
     8761 gatcgtacct cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa
     8821 gtagtaaaag taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa
     8881 ctttactgcg aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa
     8941 atcgttagcg cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc
     9001 gatttacctg tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg
     9061 aaatttttta gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc
     9121 gctatctaca gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt
     9181 gtgatgcctt tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga
     9241 cattatgtct caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt
     9301 tttgcgattg cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg
     9361 atatttattc agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat
     9421 gtacctaggc gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac
     9481 tggatctgtg ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga
     9541 gtagcattca tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat
     9601 attcccggga tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc
     9661 gttactgaat cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga
     9721 gcaatcgctt taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa
     9781 gtaaagcgtg acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt
     9841 aacaaagaaa aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa
     9901 acattgcgac aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt
     9961 tgtaaaaata gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt
    10021 tgaacaccct ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc
    10081 tttattcaaa ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta
    10141 ttagaactaa accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca
    10201 ttccgtataa gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta
    10261 aacccaaact tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca
    10321 gcaaccgata atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac
    10381 caaatagcaa gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg
    10441 cgatcccacc agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat
    10501 attggttcat agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa
    10561 taagattgct gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca
    10621 ggaaaacaac accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg
    10681 caaaagcaag aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca
    10741 aattaggttc ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt
    10801 tctttttatg ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa
    10861 gctttggtac tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta
    10921 ttccccaacc caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca
    10981 ataatatcga aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat
    11041 tcatccacat gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa
    11101 ctgtttttct atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag
    11161 ccagacaaaa aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa
    11221 gcaaaatccc tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa
    11281 caaaaggaat gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc
    11341 caaaaggcag aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga
    11401 ctgatatact ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac
    11461 ctggcaattt aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca
    11521 gccctgaaaa aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt
    11581 tctctcttgc tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt
    11641 ctaataattg tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc
    11701 aaataaatct tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc
    11761 tgataaatca attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta
    11821 tccaatacat gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt
    11881 tctttcaaat caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa
    11941 atatctggat ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa
    12001 aaacttgcga ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct
    12061 aaacgctttt cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat
    12121 tctccagccg tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt
    12181 ttgatactca atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat
    12241 ggttcaataa tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct
    12301 aaaaatccaa aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt
    12361 ttcccgtaac ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt
    12421 attacttttt attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca
    12481 aaaaaggata gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc
    12541 ttctaacagg taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc
    12601 ttgcataaaa ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt
    12661 tgcaaatgtt actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat
    12721 ataagctttt aaatctttac ataaatactc atattcgagt gattttttat attttttaat
    12781 tgtgccgttt acgagaatct cttcatcttt caatactagg cctattttat ttttataacc
    12841 tggcatgctc attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata
    12901 acggacagtt gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt
    12961 caataagtaa ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata
    13021 accactaggc gtaatctttc gattaataac agttattgct ttattcggat gagacgttag
    13081 ataaacaatt ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct
    13141 ataataggcg ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt
    13201 tttaatgaat tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg
    13261 acttaataaa attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact
    13321 atcatccaaa ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat
    13381 caaaatcatt tcatacaact tttcaacata catgttagga ataaattgta attctcttca
    13441 caatcttttc caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa
    13501 taaatatcaa acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt
    13561 gtcagctgtc tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact
    13621 gatttttcgg agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct
    13681 tgaatggttg ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt
    13741 taaccgttac tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac
    13801 aatggataat gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt
    13861 aaacaaatcc ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta
    13921 gctccataac ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact
    13981 tttctttcaa ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat
    14041 ccggctttaa gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga
    14101 aattaaatcc tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc
    14161 ccacgattac tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca
    14221 acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca
    14281 aatacagctg aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc
    14341 gataatggac attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt
    14401 cgaaataccc agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg
    14461 aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa
    14521 tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca
    14581 gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct
    14641 gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt
    14701 gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat
    14761 ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca
    14821 tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca
    14881 tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg
    14941 tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc
    15001 acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg
    15061 attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct
    15121 tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat
    15181 cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca
    15241 cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa
    15301 ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga
    15361 ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac
    15421 gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc
    15481 aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac
    15541 ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga
    15601 catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg
    15661 tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca
    15721 tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt
    15781 tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa
    15841 aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga
    15901 aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt
    15961 tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca
    16021 tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt
    16081 tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg
    16141 attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa
    16201 attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg
    16261 gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata
    16321 cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca
    16381 ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata
    16441 agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct
    16501 cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt
    16561 ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct
    16621 gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat
    16681 ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat
    16741 tgtgaaattt tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg
    16801 aatttatggt agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta
    16861 gaaaagagga ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag
    16921 aagtcttatt tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg
    16981 gcagaaatgg cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg
    17041 atgaaggaaa gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag
    17101 gacttgattc caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac
    17161 aaatggaaga gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac
    17221 ctgcttatag ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa
    17281 acggctatgg ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt
    17341 cattttatga tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag
    17401 ctcgcatgct tttgcaacac cctgacattt tgattctaga tgaaccaacg aaccatttag
    17461 atatcgagac actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga
    17521 ttgtttccca tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca
    17581 gaagaaaaat gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac
    17641 agttagcaag tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag
    17701 acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa
    17761 agacattaga aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact
    17821 ttctattcag tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta
    17881 tcggatatga taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag
    17941 cgattgctct agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc
    18001 accagatccc ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt
    18061 acgatcaagg tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg
    18121 agcatcctac cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg
    18181 gcgaagatgt agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt
    18241 tagccaagtt agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt
    18301 tagatattga caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc
    18361 tttttgtttc ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct
    18421 ctgaaaatgg cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg
    18481 aagaagaaga aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca
    18541 cgaacaaatc tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac
    18601 gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg
    18661 aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc
    18721 aagatttaga agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa
    18781 gcttggaatt agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct
    18841 gttttggttt ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca
    18901 ataagaagac caattactag tcattctcac taaagcttct aaataatagt aatcacccca
    18961 aatacaaggt gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa
    19021 gccatcttct tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac
    19081 agcagactgg tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa
    19141 taatccacaa accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc
    19201 aaagatcaaa tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc
    19261 taatattttg cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa
    19321 accgtaaatc ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg
    19381 cgtacttcca tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga
    19441 accatcttct cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt
    19501 atatttttga tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca
    19561 atccatgatc attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac
    19621 tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt
    19681 catttttcga tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc
    19741 aagatcgtga tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc
    19801 tttttcagct gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat
    19861 tcctgtccaa aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat
    19921 aggacactcg cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt
    19981 gtgtctgcga caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca
    20041 ttcatttatc catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc
    20101 cccaccagcc tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc
    20161 ccggttgtcc catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc
    20221 cgatcttgat gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc
    20281 tctaatccag gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata
    20341 atagctgtgg attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca
    20401 gcttcactca ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga
    20461 gaacattcaa tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg
    20521 tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa
    20581 gtaggttccc atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt
    20641 cgaacgatga actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat
    20701 gtgtccgctc gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc
    20761 gctcgcggta tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg
    20821 gtcaatgtat ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta
    20881 tcatgttgta tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata
    20941 ggcatttctt tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc
    21001 ttattcagcc ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg
    21061 taggtataac caatattcag gatacctttt tcatcaaata tttctttttg catccaccat
    21121 cttatattcc gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc
    21181 cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa
    21241 ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct
    21301 cggttcttat atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca
    21361 taataatgaa atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac
    21421 cagccatttt gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct
    21481 ttaactggat aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa
    21541 ttaccatctg cacattcata ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt
    21601 tctcctgtat ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt
    21661 gcgggcattt ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg
    21721 gaaggatctg ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat
    21781 tggtcaattg cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt
    21841 ttatttccat atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa
    21901 aacggctgga gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt
    21961 aatagtggta ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt
    22021 ccgactgttc taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca
    22081 cattataagt cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat
    22141 ccatattcca atttgcatag tgcttttctg tcttttggag tatattgata gcagcttcat
    22201 gatacatttt tgatcgtaca gaggaaacat atcctgctaa ttccagaaga ccgcatgcag
    22261 cgcatactcc agcactggta tcaatattcg aatcttttac agcaaaatca atgagaggaa
    22321 tataaccagt tttttcgatt ttctgaataa aatttactgc tatcttttct gctatagcta
    22381 gatagtacgg attttttgtg taacgataac ttaacgcgaa tccatataaa gcccatgcat
    22441 ttcctctgct ccaacaagaa ttctcattgt atccttgccc gccaagtaac cgaatatatt
    22501 tccctgattc tgcatcaaat tctgcgatat gcccggttga tccatctttc ctcactagat
    22561 acttagctgt tgtatctgca tgctgccgag caattttaga gaacctcgga tcaccagttt
    22621 ccctcgaagc ccaatacagt aatggtaaat tcatcatact atcaataatc atccaaccag
    22681 gtttatggtc attccatgct actaaatagt tccctgaaat attatagcgt ccagcaagta
    22741 ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt ttgagactgt tgataatgtg
    22801 caatagccga tggtaagaat aaaaaaccta catcatgatg aacgtctgaa aaatggtaca
    22861 gggcagattc taataattgt tcttgttgta ttgctgcctc tttgaacttt tctcgttttg
    22921 tcttgtgaaa gagctgccat aaaataccac tccaaaaacc attggtccac cagtcgattc
    22981 ctctgtcctt tattacgtct gtatattttc cgttgtaaga aatatatgga attttattcc
    23041 ccattctgtc taattccgca tctatctttt cagaagctac attcaaaatt tttcttgacc
    23101 attccttcgt ctgttcattc atggatatcc cgccattctt ttaattttta tgaatgtgga
    23161 gttgctccta gaacgaacta gatggttaac attcgttatt ttttttactt atttgcagct
    23221 ccaatgtcat tctccttaaa tttagactgc gcccacacat aaatcaagac cattgggatc
    23281 actgaagttg ctaaaattag cataattgcg ccataattaa ttggctcacc tgtactagaa
    23341 accgtcatat ttttgataat taagggaacc gtatatttat ctttatctga aagcatgact
    23401 aatggaaata gatattgatt ccaacttccc ataaagataa gtaaaccact agtaataatc
    23461 gtaggtgata aaatcggtac agcgattttc gtaaaaatca cccactcatt ggcaccatcg
    23521 attctggcag cctccatcag tgctgtagga aattgactgc tatattgtcg catcataaac
    23581 actgaagttg tactggcaat cgaagggatg atcacacctg cataagtatt cgataatcct
    23641 aaacgactca taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac catcctagat
    23701 agcataactg tattgaacaa cagtttattc cccataaaac gatactttgc taatgcataa
    23761 cccgccattg tagaactggc tgtagctaaa actgtactga ttacagcaat aacaatcgaa
    23821 ttccaaataa tcaagggata attatatctc gccatgatgg cttgccagtt ttttaatagc
    23881 atatccccaa acttgaaatt gattccgcca cttaaaattt cttgattact ttgcgttgaa
    23941 gctacaatca tatataaata tggcatcaaa aatattaagg caccaaacac aataatgacg
    24001 tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt ttatcacctc cgaatttaaa
    24061 ttgaatagcc gataatataa cagatacaaa caccatcgtc caggcaatag cagaggcttt
    24121 tccaaaatca accatttcaa aacttgtttt atataaattg actcctacga ccaaagctga
    24181 attttctggt ccattggaag tcccaaagaa tatatttggg atattgaaat tacccaagcc
    24241 attgatagtg gctaaaactg tggccattaa aatgattggc tttagcatgg ggatcgttaa
    24301 taaaaagaat tgccgaaatt tcgatgctcc atctatttca gcactttcgt atatctcttt
    24361 agaaatccct tgaagaccag ctaaaaataa aatagtgtta tatcctacac taagccaaat
    24421 catcgtgatg atcattgata ttcgtatcca aaaaggattt gttaaccact gtacaccgtt
    24481 aattcctagc gcattcaaaa atccattgaa taacccagtc ggattgaaaa aagtcatgaa
    24541 tacggaagct acagccaccg ttgatgtaat ggttggaata taatagacag ttctaaaaaa
    24601 tccttttcct tttaactgtg ctgaattcaa taaaacagca aagtaaatag ctaatagcat
    24661 cgttattgga attgatcctg ccatgataat caaaacattc gtgtaagatt taataaacaa
    24721 tggatctcgg aagatagcta gataattgtc aaatccaaca aattcaaatg agtagccaat
    24781 tttcataaaa ctaaaagcaa acgagataat gatgggaatc aaaatcgtca caataaagaa
    24841 aataacaaaa ggtgtgacca atccataagc acttttttta tgagacgtat ctgttgcttt
    24901 tttcttgctg ttctttattg tcttgcttgg taacgaagat tgtatcatcg ttaagtcctc
    24961 ctaatatttt ttaatcacca gttaatttta cgccgtactt atgagcaaaa ttttctgctt
    25021 cttttagata tgaattttca aaatcacctg tttgccaata gttatatgca gcggtagtca
    25081 aataagacgt agcatctgaa taagtggctg gtaaaacagt atttgatgta ttttcattga
    25141 ttttattcaa tagttcatag tatttttgtc ctccttagta gtcaaacttt ttctcagctg
    25201 cttctgtttg ataggccaat gagttagatg tagccacgcc cagctccagt gcgttggcta
    25261 agcattcttc atctgtcagc ataaatgtaa gaaattgttg tgccacttgt ggatttttgg
    25321 catctttagg gacataaaaa cttgatcctc ctgatactgc accttttcct ggattttctt
    25381 ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga tgcatcagga aaattcaaag
    25441 acatattcgt agcataccag ccgccagcaa caaacattgc agattcttga aatgctttct
    25501 cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc gatcatttct ttgattaact
    25561 ttgcagcatg tttcgcttct ttagtattca gacggatatt tccttcttca tcaagcaaag
    25621 attgattctg ctgggctagc atgttgataa acaattcttg gtccccagat tctggcaagg
    25681 caattccaaa actaccagtt ttttctttta atgcttttgc cacctggata tattcttccc
    25741 aagaactgat ttcttcaaaa tctacaccta tttcagcaaa tttttttggt tgataataac
    25801 aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt ttcattactc gtttgttgac
    25861 gcaaaatatt caatttttga tcataaaact gatcgccata tttgtcataa aatccatatt
    25921 catcagcact ataaaaagca tcttgaaatt tttccagcac acctgctgct gaagtgtctg
    25981 ccatggagat aatatccggc atatcttcgt gtgttaccaa ttttggggtg atcatttgta
    26041 ctgttgctgt tcccgaagct acattcacaa tttttaagtc aatatttgct ccagttttgc
    26101 ggttatagat ttttgcagca ttttcaacat agtttgtcag tttgccgttt gcgacatata
    26161 cttctaatgt agtggctttt tttatcattg gatctgcgat gtttgctgtt acttcttcgt
    26221 tagtttttcc acatgcactt aagctgaata atagggtaat gtttagcaaa gctatcacca
    26281 atttgtatct ttttttcatg tctatccctt ctttcttttc aagttcttat ataaaacgct
    26341 ttcacttatg tatattaacg gaattagtaa cacaaatatt tatataaaaa aatattttac
    26401 ttgtcgtttt ggcttttcat ttttgttata atagtcatta attataaata gaaagctttt
    26461 tatataaaaa gagaaaggaa agtggttctt ttgttaacaa acaaccaatc ctccaatctt
    26521 gtcaaaaaaa cactaaccta ttccatagat acccatcttc atttatctgc tgctccctat
    26581 tttcgactac taataaatga aagcgctaat ttatatgctg aatttgcaca taaatctata
    26641 gaactagata atccttccct tttattcatt gataaacagc aagaagtcca aatcttttct
    26701 actcaaaagg aaacacaaac ttttatcatt atccagtttt attttgatga gcaattatta
    26761 gcacatatta cgggacaatc tttttctata gatcaagaaa taacatctat cattactttt
    26821 ctgaaaaatg aacagttcaa aattgattat ctaaaacata gtaagccagc tatttcctat
    26881 gatcaggaaa tcaattcttt tttcatacta tgccaacaga tgagcattca tttgctttcc
    26941 ttattagtta cttattgtct gcaaaaaatt caattgtctt ccttaccaag gcattatcaa
    27001 caggaacttg ctttattgac aataccttca aatgagaaag tcattcctaa aaaaaagcat
    27061 attctttatt cagatgaatt agttgatcca atcatacgat atttagaaga acatctttca
    27121 gaaaattttt tgcttgaaca attagcccaa atttttttta tcagtccagc cagcttaaaa
    27181 aaacatttta gaaaagtgaa tggaaaaagt gttattagct attttaaaga actaaaaatg
    27241 gaaaaagcaa aacgtctcgt gctagaaaat gaattaacct atacagaaat tgcagatata
    27301 ttaggatttc attcgatcca tcatttttct tcagctttta aaaaatatac agggttatcc
    27361 ccttcaaagt attatctatc cttaaaatag aaaaaaattt ttaggtttta tccacaaatt
    27421 atttctctat attttctgtc tattcaaaat aaacaataat tttccggcac aattatttgc
    27481 tgttttttac cataacatag tacgatcaac gtagaaaagg agatgttttt atgaaagttg
    27541 ttgtcattgg cggtgtagct ggcggtcctt catttgccac tcgtttccgt cgattgaatg
    27601 aagcgcacga aatcattatc tatgaacgcg gagaaaatat ttcttatgca agttgtgctt
    27661 tgccttatta tttaggaggt gtgatcactg accgtgactc gctgatcgaa cgtacaccag
    27721 aaatattgaa aacaaaaaac aacatcgatg tctttactaa acacgaagta acagcaatcg
    27781 atccttctac taagcgatta acagttaaag acctatccac aaatgaagaa ataaaaacag
    27841 attacgataa gttgatcatc tcttctggtg ctagaccaga ttatccggat attcccggag
    27901 tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat
    27961 cgttccttga agaaaaaaat ccacaacatg tcgtcattct tggtgcaggt gttatgggtc
    28021 tggaattagc tgagaatttc aagcatcgcg gcttagacgt gactttaatc gatcaattgc
    28081 cacaagtcgc tttcccttat gatccagaaa ttgctaattt agtttatgac aaattgctga
    28141 aagaaggatt agccgttcat ttagaaacaa gagttactga gatccgtgat aaaggtcgag
    28201 aaatagtatt atcagatggc tccgtccttt ctgctgatat gctaattttt gctgttggtg
    28261 tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca attatcagat acaggtcaga
    28321 tcattgttga tgaccagtta caaaccaatc tgccggacat ctatgcgatt ggcgatatta
    28381 tcgaaacgac tagtgtagtg actggtcagc cgatccaaag tatgctttcc agtgcggcca
    28441 atcgtcaagg acatatgctg gcagatattt taaatggtac gcctatgcgc tatcgcggat
    28501 atattggtgc aggtgtcgca aaaatctttg atcatacagc aagttatgcg ggaatgacag
    28561 aacatgaatt aaaagcatca ggcataacaa attataaaac tgtttttatc actccctttg
    28621 accatgccta tttctatcca gaagctacaa gattaaatct aaagctgatt tttgatgcag
    28681 atagcggtcg tattttaggt ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg
    28741 gagaactttc tgtagcaatc accggaaact tgacagtctt tgatttgcct gatttggagt
    28801 tgccttactc tccaccgtat tctactaccc gtgacccgtt gaatatagct ggttatgtcg
    28861 caatcaatca aatgacgaat atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg
    28921 atttgaaaga agcgttcttt ttagatatac gtgaacctaa taaagcacct tctggcagta
    28981 tctcggcaac aaaaaatatt ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa
    29041 aagataaaaa agtttacatt actttcagaa gaggattgaa tacttatact tctgcccgaa
    29101 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
    29161 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
    29221 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
    29281 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
    29341 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
    29401 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
    29461 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
    29521 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
    29581 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
    29641 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta ttta
//
LOCUS       DACECB010000029        29572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECB010000029 DACECB010000000
VERSION     DACECB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.029"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWO04_001391"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWO04_001391"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0213012.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWO04_001392"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWO04_001392"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0213013.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWO04_001393"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWO04_001393"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0213014.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IWO04_001394"
     CDS             complement(3376..4683)
                     /locus_tag="IWO04_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0213015.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IWO04_001395"
     CDS             complement(4686..5267)
                     /locus_tag="IWO04_001395"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0213016.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IWO04_001396"
     CDS             complement(5839..7131)
                     /locus_tag="IWO04_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0213017.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IWO04_001397"
     CDS             complement(7128..7751)
                     /locus_tag="IWO04_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213018.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IWO04_001398"
     CDS             complement(7754..8590)
                     /locus_tag="IWO04_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213019.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IWO04_001399"
     CDS             complement(8607..9494)
                     /locus_tag="IWO04_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213020.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IWO04_001400"
     CDS             complement(9510..10793)
                     /locus_tag="IWO04_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0213021.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IWO04_001401"
     CDS             complement(11476..12159)
                     /locus_tag="IWO04_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0213022.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IWO04_001402"
     CDS             complement(12177..12794)
                     /locus_tag="IWO04_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0213023.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IWO04_001403"
     CDS             complement(12804..13628)
                     /locus_tag="IWO04_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0213024.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IWO04_001404"
     CDS             complement(13621..14181)
                     /locus_tag="IWO04_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213025.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="IWO04_001405"
     CDS             14355..15311
                     /locus_tag="IWO04_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0213026.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="IWO04_001406"
     CDS             complement(15616..16572)
                     /locus_tag="IWO04_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0213027.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="IWO04_001407"
     CDS             16676..17278
                     /locus_tag="IWO04_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0213028.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="IWO04_001408"
     CDS             complement(17366..18865)
                     /locus_tag="IWO04_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0213029.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="IWO04_001409"
     CDS             complement(18881..19405)
                     /locus_tag="IWO04_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213030.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="IWO04_001410"
     CDS             19703..20452
                     /locus_tag="IWO04_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0213031.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="IWO04_001411"
     CDS             20443..21228
                     /locus_tag="IWO04_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0213032.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="IWO04_001412"
     CDS             21197..21913
                     /locus_tag="IWO04_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0213033.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="IWO04_001413"
     CDS             complement(22015..23034)
                     /locus_tag="IWO04_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0213034.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24111)
                     /locus_tag="IWO04_001414"
     CDS             complement(23053..24111)
                     /locus_tag="IWO04_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0213035.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWO04_001415"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWO04_001415"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0213036.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="IWO04_001416"
     CDS             complement(25556..26392)
                     /locus_tag="IWO04_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213037.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="IWO04_001417"
     CDS             complement(26576..27793)
                     /locus_tag="IWO04_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0213038.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28176..28259
                     /locus_tag="IWO04_001418"
     tRNA            28176..28259
                     /locus_tag="IWO04_001418"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..29497)
                     /locus_tag="IWO04_001419"
     CDS             complement(28499..29497)
                     /locus_tag="IWO04_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213039.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8844 a         6303 c         5063 g         9362 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgtctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
    29401 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
    29461 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
    29521 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DACECB010000030        29321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECB010000030 DACECB010000000
VERSION     DACECB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.030"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(128..1519)
                     /locus_tag="IWO04_001420"
     CDS             complement(128..1519)
                     /locus_tag="IWO04_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0213040.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1732..2118)
                     /locus_tag="IWO04_001421"
     CDS             complement(1732..2118)
                     /locus_tag="IWO04_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0213041.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2174..2797)
                     /locus_tag="IWO04_001422"
     CDS             complement(2174..2797)
                     /locus_tag="IWO04_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0213042.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2861..3529)
                     /locus_tag="IWO04_001423"
     CDS             complement(2861..3529)
                     /locus_tag="IWO04_001423"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0213043.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3541..3981)
                     /locus_tag="IWO04_001424"
     CDS             complement(3541..3981)
                     /locus_tag="IWO04_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213044.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4040..4711)
                     /locus_tag="IWO04_001425"
     CDS             complement(4040..4711)
                     /locus_tag="IWO04_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0213045.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4896..5294)
                     /locus_tag="IWO04_001426"
     CDS             complement(4896..5294)
                     /locus_tag="IWO04_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0213046.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5291..6064)
                     /locus_tag="IWO04_001427"
     CDS             complement(5291..6064)
                     /locus_tag="IWO04_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0213047.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6061..6744)
                     /locus_tag="IWO04_001428"
     CDS             complement(6061..6744)
                     /locus_tag="IWO04_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0213048.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6911..7798)
                     /locus_tag="IWO04_001429"
     CDS             complement(6911..7798)
                     /locus_tag="IWO04_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213049.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7795..8664)
                     /locus_tag="IWO04_001430"
     CDS             complement(7795..8664)
                     /locus_tag="IWO04_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213050.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8801..10087)
                     /locus_tag="IWO04_001431"
     CDS             complement(8801..10087)
                     /locus_tag="IWO04_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0213051.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10360..10938
                     /locus_tag="IWO04_001432"
     CDS             10360..10938
                     /locus_tag="IWO04_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0213052.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10938..11867
                     /locus_tag="IWO04_001433"
     CDS             10938..11867
                     /locus_tag="IWO04_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213053.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11864..13600
                     /locus_tag="IWO04_001434"
     CDS             11864..13600
                     /locus_tag="IWO04_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0213054.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13663..14499)
                     /gene="pheA"
                     /locus_tag="IWO04_001435"
     CDS             complement(13663..14499)
                     /gene="pheA"
                     /locus_tag="IWO04_001435"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0213055.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14496..15005)
                     /locus_tag="IWO04_001436"
     CDS             complement(14496..15005)
                     /locus_tag="IWO04_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0213056.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(14998..16293)
                     /gene="aroA"
                     /locus_tag="IWO04_001437"
     CDS             complement(14998..16293)
                     /gene="aroA"
                     /locus_tag="IWO04_001437"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0213057.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16306..17397)
                     /locus_tag="IWO04_001438"
     CDS             complement(16306..17397)
                     /locus_tag="IWO04_001438"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0213058.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17414..18580)
                     /gene="aroC"
                     /locus_tag="IWO04_001439"
     CDS             complement(17414..18580)
                     /gene="aroC"
                     /locus_tag="IWO04_001439"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0213059.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18582..19646)
                     /gene="aroB"
                     /locus_tag="IWO04_001440"
     CDS             complement(18582..19646)
                     /gene="aroB"
                     /locus_tag="IWO04_001440"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0213060.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19679..20701)
                     /gene="aroF"
                     /locus_tag="IWO04_001441"
     CDS             complement(19679..20701)
                     /gene="aroF"
                     /locus_tag="IWO04_001441"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0213061.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21360..22334)
                     /locus_tag="IWO04_001442"
     CDS             complement(21360..22334)
                     /locus_tag="IWO04_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0213062.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22315..24198)
                     /locus_tag="IWO04_001443"
     CDS             complement(22315..24198)
                     /locus_tag="IWO04_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0213063.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24216..26441)
                     /locus_tag="IWO04_001444"
     CDS             complement(24216..26441)
                     /locus_tag="IWO04_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0213064.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26556..27590
                     /locus_tag="IWO04_001445"
     CDS             26556..27590
                     /locus_tag="IWO04_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0213065.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27657..28670)
                     /locus_tag="IWO04_001446"
     CDS             complement(27657..28670)
                     /locus_tag="IWO04_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0213066.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28748..28936)
                     /locus_tag="IWO04_001447"
     CDS             complement(28748..28936)
                     /locus_tag="IWO04_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0213067.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         8437 a         6348 c         5179 g         9357 t
ORIGIN      
        1 attcacggtg tttcagaagt agcttctcgg aaataagccg aaatttcaca aaaatttgag
       61 aaactatttt taggaaactt cttcttattt cttaaagctg accacttcta tcacaacctc
      121 tttttgacta tgcattcaca tcgatcactt tagaatcatc attgatgaca tcgcgatcta
      181 aatctttgat gcgtgcactt gtcacgatac cagctaccat gctaccgctg acgtttactg
      241 ctgtacgtgc catatcgatt aatggttcaa cagagataac taacccaacg attgctactg
      301 gcaagttcat tgcccctaaa acgatcagtg aagcaaatgt tgcgccccca ccgactccag
      361 ctacaccaaa agaactgatt gttacaattg ccacaagcat caaaataaat tccaaactaa
      421 agacattcac tccagctgct ggtgcgacga tcgttgccaa catagctgga taaacgccag
      481 cacagccgtt ttgtccaata gacaaaccaa aacttgctga gaaattagct gttgcatcat
      541 ctactcctaa agcttttgtc tgagtctcga tattcaatgg caaagctcca gcacttgaac
      601 gtgaagtaaa cgcaaagctc aataccggaa aggctttttt gaaatagttg attggattga
      661 cttttgcacc aatcaaaatc agtgagtgga caatcaaaac agcaagcaat gctacgtaag
      721 aagcaacgac aaatttacct aaattgacga tagctgcaaa atcacttgtt gccaaaacgt
      781 ttgtcatcaa agcaaatact ccataaggtg tcaaacgcaa aacaagcgtc acgatccgca
      841 taatgatttt atacagactg tcgactaaat ctgaaaagaa tgctgcttct tttggcgctt
      901 ttcttttcac gcctagataa gcgattccga caaatgccgc gaaaataacg actccaatcg
      961 tacttgttgc acgactacct gctaaatcag caaaaacgtt tttagggata aaggataaaa
     1021 tctgctgtgg aatcgataag ttttcaactg tttcctgacg tgttgccagt tcagagattc
     1081 gtgctgtttc tgcagctcct tgtgtgaatt tagctccttg gagtccgaac agcattgttg
     1141 aaccaattcc gatcaatgct gcaatagctg ttgttccaag taaagaagtc aatacagtcg
     1201 cactaatttt ccctagcttt tcagaaactt tcatttttgt gaatgcacca atgatcgaaa
     1261 taaacactaa aggcataact agcatttgca agaatgatac atatccttga cctacaatcc
     1321 cgatccattc cattgattga gtaacgactt tgtcgtctac accaaagatc aattgaagca
     1381 gaccgccaaa gacgatacca actcctaatg ctgtaaagac gcgtgttgaa aattttgtat
     1441 ggcgcttttg catacgatag aacacatata ataatgccgc aaaaactaga accactaaaa
     1501 ctgtgatgag tgttgtcatt tttctcctcc tcatattatt aatgacaagt ccatcgaagg
     1561 aagtaaaggt tcccgagaat gattcttcaa ccatcctcct gaaaaaattt ttttcagtga
     1621 cttttatatt ttagacgatt tttatcgaaa tgacattctt tttgcttaca attagtaata
     1681 aaaaaacata cctgtgatag ctttaatcta tcgcaggtat gtttttactg tctattcttg
     1741 tttttctgat tgaaaattca attttacccc aggctgatct aaggcgatct ctgtcacctc
     1801 ataagacatt cggcgtacga tatcgaataa cggtgctact agctcgtcca cttcttcttg
     1861 tgtgacatca ttttgacctt caattttccg attgaggata tgattgatct gactcacact
     1921 tccagataaa acatatgtat caaacactaa acggaactct aagcgtgctc ctacgatgct
     1981 atgttctttc ggatagtttt cgatcgtttc ttttaaaggc gagaagccta ctttcaaatc
     2041 tgtttctact tcttggttcg gcaatctcat atcataatga aatgcttcta caacttcttt
     2101 ttgtcttctg atttccatat aattttatca ctcctttatg aaaattataa catatctcct
     2161 ttgaaagggg atactacttt ttaatgatgc tctgcaaggt aaggatttca ttcagatttc
     2221 ctaccacata atctgccggg atctcaccca gaaaatgatc gatgtcgtaa agacaagtag
     2281 cgactcctgc atttttccct gcttcgatat ctaaacgacg atcgccgatc atcatcgtgt
     2341 ctgttctttc taaatgaaaa gtatcgatca aataatttaa agatgctgga tcaggtttac
     2401 gaggaaagtc ctgatcgatc cctactactt cctcaatcag atgtgccaat cgatgttctt
     2461 ttaacaggcc ccatgttgat tctgtcaaac ggtgggtcag gatgaaatga cgaccgccgt
     2521 tgtcttttag catttccagt acttgtttcg tttctggaaa tggacgggaa acttccgtac
     2581 tttctttttc atatcggtga aataattcat taaactctct ggcattcaag tttgattcat
     2641 tgatcaaata actagtagaa tactttttca ttttgaagta gatttctttt ttgtccatac
     2701 tgataccaaa atcttttagt gcttgccatg caccatctac cattgcggga tacgtatcaa
     2761 acaatgtacc atcaaagtcc catatataat ttttcatgtt ccttttgttc cattcctttg
     2821 cttctttttt ctatctctca cttttagata aaggtgttct ttaaagtatt tttttgtcca
     2881 gaattgtcaa atcatctgct aatgtatact ggattggttg tgcatttggg atatcgatcg
     2941 tatccatttg atcttcagga acatcttcta aatattttgt cagtgctcgg atgctattac
     3001 catgacccac aactagtaaa tcttgccgat cttttagttt tggtgctaga tgatcttgcc
     3061 ataagggaat gatccgattc actgtcattt ccagactttc tccgtatggg atcaaatgcg
     3121 gatccaagtg atcatatctg cggtcaaatc tattttctgc ggttaacggc ggaacttccg
     3181 tatatcctct gcgccatgtt tttacttgat cttttccgta tatttctttc atcttatctt
     3241 tgtttttacc cacaagatcc ccataatgcc gttcatttag acgccatgtt tttattatag
     3301 gaagccataa ctgacccatt tcttctagtg cgacttgtcc ggtaagaatc gcacgtttca
     3361 aaacagaggt ataaacggca tcgaattgaa gtccggcttc aagcattttt tgaccggcaa
     3421 ctgctgcttg ttcttttcct ttttctgtta aggcgacatc tagccatcct gtccagtaat
     3481 tttcaaaatt tgcttcgctt tcgccatgac gtaataatac taatttcatt cttttctccc
     3541 ttaaacattc gtttcgaatt ttataccaca tgttttgcat gtcacacgta ttcgtccttt
     3601 tcctttaggt gcacgctgct tttgatgaca attcgggcac tcaaaaaaag tataactttt
     3661 tcgatccatt ttgacttgct ttttgttttt tatctgatga tatttttctt tgactctatc
     3721 aagatatttc aaataacgtt gattctcatt tagtctgggg tagatttttt ttgataggaa
     3781 acgaaatatc atccatccga aaaagacgat gaacaacaat tgtccgattc tatagggcaa
     3841 ccaacgactt agcaaactaa agacaagcgc agcgatcaat agaccgcgat tcaactgatc
     3901 aaatttcgca tatcttccct gcataagttt gtgatagttc tgctgaaatc gtattagcct
     3961 ttctaaccat gtttgcatca tattatctcc tccatcatct aatatttatt atgacaaaaa
     4021 aatccaaaaa gaaaaagcct taattggcct tttccattgc aacttggtga aggacattcc
     4081 gatttctggt aaagtcaatc acaatcactt tctttttatc ttctctgttg actactcgaa
     4141 atgtgtccgt caatggaaga aacagcttct tagatggtag ctgttgcata cgtataaaac
     4201 gatggatcat cgtaatcaat tgaggaaatt gaatagacgt gtgaagcaga cctggatatt
     4261 ttttcaacag ctgtgtcgtt ttgatcagat tccgtttttt actgatcact tttaaaaagc
     4321 tttttagaat cttttgctga cgtctaaaaa tagaaaaaga attttcgtca tctatttgat
     4381 aacgcatttg tttttgcagt tctgctgcag tcagatggat cttcccatcg tttgtatcga
     4441 tcattaaacc ttctggaaac agttcaacca ctgctcgatc ccaagattca agagaaagct
     4501 cgatataatc ttttactggt aattccaatt ctaccttaaa aaagtgcaaa acagccgaca
     4561 gcccttctgc ttgatagatt tctcctaatg ttagcccctc tagatggaac tctggtgaaa
     4621 aatgcagcaa tgttttattt tcctgcaatt gaacatatag aaatgtagat cgctcaatca
     4681 gagtatcttt taaaacaact aaaaaattca tttttctccc ccttttttca tcatttttaa
     4741 aatagcatat tttgaaaaga aagtctgatg cttagagtag aatttcataa aaatttacta
     4801 tttcataagt attcacgaga aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct
     4861 tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg attcagactt ttctctatcg
     4921 ttattttcat ctaaaagttc ttgcattccg ataatttctc gatattgatg aatcttagtg
     4981 ataccaatga ctttgtctac atcaatacta aaacaaatac cattacctgg aatatcgatg
     5041 ttgatgcctt gaccaatcgc atccattact ttattcgtca aactatcggg tactaatgtc
     5101 aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa atttccctga atcatgtaca
     5161 ccagaacctc ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg
     5221 ataacatctt cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct
     5281 acaagatctt tcatttgctt tcccccaatt tttgcaatct ttctttttcg attctgtctt
     5341 tttcatcttt tcgtttataa atatatccta gtactaaagt ggataatatt ggtgtcaatg
     5401 caatcattgc aaccacacca aacccgtcaa tgatcggatc tcttccttca atttgtgcag
     5461 cgattgccac gctcattgat aaaataaatg tcgaacacat cgggcccgtt gctactccac
     5521 cgttatcaaa agccattgct aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta
     5581 gcccgtatcc tgggatcaag aaataataaa gagagaatcc gacgagcaaa cgccacatag
     5641 acaatcctac tgctgcgccg atcccaattg aaattaccat cagcatgatt ttcgatcgga
     5701 ttctcccctc gctcaattct tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa
     5761 accctactaa gaaccccatt aaaaagccaa ggggaaccag tatataattg taaggcaatc
     5821 ccgcaatagc actgcctaaa tgctgtccta ccggtacata agccatattg actccgtgca
     5881 ggaaaaggat caacccaata gtcgttatca agaaaccttt cataatgtca aagaaacgct
     5941 ttttgcgcat acgaaaacta atgatattca ttaaaacaaa taggattacg ataggtgcca
     6001 ctgccaaaat gacttccatg gtaacgattt cgattccatc tatcacttgc tgccagaaac
     6061 tcattggaag atcaccccca aaatcatcac ggcaagaatc ggccctaacg aagcaatacc
     6121 gaccattcca aaactgtcat tttcattttt cttttgtttg atcatactag tcatccctcc
     6181 ggcaagggcc aagataaatg gaacagttac tggaccagta gtcactcccc ccgaatcaaa
     6241 agcaatcggc ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc
     6301 tatagccatc aattgataat aggatagctt gaatacgacc cgaagcagcg caaaggctag
     6361 aaatactcca gtgccgacgc tgacgatccc aatcagcaac actctgccaa tctctccgtc
     6421 agatatatca tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac
     6481 gatcccgata gcaaatccca aactgaccaa aacggtcaaa cttttctttt tgatcatata
     6541 tttccctact agatctccga cttccatcat agaataatct gctccaaaca aaaagagcgt
     6601 cattccgatc atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa
     6661 cggtgcaaaa acaaacgtta aaatgacaat caggacagtc atcggcaata tagcagcaat
     6721 cacttcttta aaatttttta gcatgtatcc tcctccttta ctctttaagt ttaccttaaa
     6781 atgaaagagt tttcgagcac aaacttaaaa cgttatcatt taatttttct caacataaaa
     6841 taacacacgt cccaaaagta tctttttact tggaaaaatg actttgaagg aacgcgctta
     6901 ttgtgttttt ttattttaca gagccgatca ttccttgaac gaattgcttt tgcaaaacaa
     6961 aaaatacgat ggcagttggt aaagtagcga tgactaatcc agccatgatc attccatagt
     7021 ctggtgtata ggaagcaccc atagcagata aaacaagcgg aaccgtccgt ttatcaggag
     7081 attgcaaagc tactaagggc cataaataat tgttccagct actcatgaat gtgatgattg
     7141 cagccgcggc ataggtattt ttagccgtcg gcatatacac tctaaaaaaa atacttagtt
     7201 cttttagacc gtctaatcga gcggcttcta ctagatcttt tggaaaagat ttaacatttt
     7261 gtcgaaagaa gaagatcaaa aaagcagtgc taacagatgg caggatgaca accaagaaac
     7321 tgttgattcc caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa gggatcatca
     7381 atgccgcaaa aggaaccatc atcgatagca gtagaatatt gaaaattagg tcttttcgtt
     7441 tactttgata aatctcaaat ccatatcctg ccattgacga aatcagcaat gcaaacacag
     7501 tggtcaccaa agcgatcatc gctgaattcc aaagggaacg agtaaaattc agctcattgg
     7561 aaaacaaatt ttgaaaattg atgatcaagt tactgccaat ctttattttt cccgatgtga
     7621 tatccacggg tgtattcgtc attcccaaaa ccatccagac aaaagggaaa atggaaataa
     7681 atgataggat gcttaatgct gtatacttta atactgaccc aattgtcgaa cgaagttcta
     7741 atgcttgttt tttgacgttg gggcgttcga ttgtttcttt ttctaccata tttttcatcg
     7801 actttctcct cctactttcg tttggattat cgacaatatc acaataaagg aaacaataac
     7861 aaaggaaact gctgccgcat atccaaaatt aggcgtgaat ttataactca aattatagat
     7921 gtattgagat agggtggtcg ttgcatttgc tggtcccccg ccagtaatat tttgtacttc
     7981 atcaaaaagc tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt
     8041 taaatttggg atcgtcactt tgataaactg ctgccattta gaagcaccgt caatagatgc
     8101 tgcttcataa atctccgaat caatattttg catgcctgaa aggaaaaaaa tcatattgta
     8161 tccagtccaa cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt
     8221 caaccagcca atcggtgtgt caatcatacc aatgcccaac aacaagttgt ttacaaggcc
     8281 agattgagaa aatagacttt tcatgatcag agaataactg accatcgaag tgatacatgg
     8341 aagaaagata gcggttcgaa acaacccttt atatttcaac tttttatcat ttaaaaggtt
     8401 ggaaataaaa agtgctagaa acaacatgat cggtacttgt acaagtaaat acaggaatgt
     8461 attaaaaaaa gcttttttaa aagttgcatc tgtcaacatc cgctgataat tacctaaccc
     8521 gttaaatgtc atattgacag ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat
     8581 cggaataaaa accatgattg atatcaaaat gactggcaag attaaaaaag catgcccgtt
     8641 tttgaataaa tctttctgct tcattctttt ccctccaatt ctatcattga ttatctacat
     8701 gaaaagacaa gaaagacaat atctgtaagg cagacgtgat cttttttgtc ttttcgtttg
     8761 tctgatgaaa aagcgaagaa attcactttt ttaccaccga tcaattagcc aattgtgctt
     8821 ctacttgttt ttgtgtatct gctagtacct tgtctgtctc ttctccattg atgattcgat
     8881 gaagagcttc agctacgact gcttcaattg cataggtctc atgtccgtaa ttcacttctg
     8941 gtatttcaga tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg taaaattcag
     9001 aagcttcttg ataagcttct tgttcattcg cgctaagcat cgtagatact agtccgattt
     9061 ctttcgcaag agtgcccatc agttcttcat tcgaagcaaa cgttgcttct aggaaatctt
     9121 tcgctgaatc ttctccagct actcctttga tcacatacca gccagcgccg ccaaggttag
     9181 aagcttgcgc tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc ttccattttc
     9241 cagattggtt ttctgctcct tggatcgttg aagaatacca agcgccagtt gggctagaag
     9301 ctactgctcc tgattgtatc gcagttactc cagcattcca gtcagaggtt tgttcgacta
     9361 gatcttcttt caaaagagtg gcaaacaatt caagaccata ctttaaagat gcattgtctt
     9421 ggatcgtgac tgtctcccca tcttcagatg tataccattc tcctgcctgt tgcataatca
     9481 tccttactcg accaaggtca gaaggattga cttcagcgat tttctttccc gtcttttctt
     9541 tgacatctcg cgctacttga atatactcgt cccacttcag attctccata tcctcttctg
     9601 tataacctgc ttctgccatc agatccgtac gatagaagtt tgctgttacg cccgaatcga
     9661 aaggaacacc gtatatttta tctcctactt tgtttaccgc aaatttatac ggtgcaaaat
     9721 gatcttcctt tacaatagca gacaaattag cgaatgcatc tggataagaa gtcaaatagc
     9781 cttgtatcct ataatcttca atcaagacga tattgggcaa tccttctgta ttcccgctcg
     9841 ctaaagcggt gttcagtttc tgaacgatat catcctgaga catcgtaaca acttctatct
     9901 ctgtttcttc gttctcataa actttttttg cttcattcac tgctttgata ttgaatgtct
     9961 catcccacgc ccagacagtg actttttctt ctttgtctgc tgtactacta gcacttccgc
    10021 atcctgttaa gaaacctgat gctgccgcta aacaaacgac tccagcaatt aactttctga
    10081 tgttcatttt tatccgctcc ttttccatcc attagttgtt tacgtttgct acattcatca
    10141 taccaaaatt tgaaaacggt ttctttatta ttcagatact tcaaaaattt ctcctaaaat
    10201 aataaaaaaa tacaaatcaa ctatgacaaa aggaataatc ttagcatcta gaccaaagat
    10261 cgaaatggtg aggtttcttt aaaataaaaa tatccttgca ttttctccta gaaataagta
    10321 tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg cgtaattctt
    10381 atcgatgatg aacagacgat tttagatgga atgaaacagt tgatcaattg gtctgagcac
    10441 gggtttcaat taatagggac tttttccagt ccgctgaaag cgcttgattt ttctttggta
    10501 catgcaatcg atatcgtggt cacagatgta atgatgcctg aattaaatgg aatcgaatta
    10561 tcgcggctat taaaacagca acaacctgaa acacagatcc ttatcctctc tagctatgat
    10621 gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg ctgattatct tttaaagcct
    10681 cgattgagtc ctgatttttt tctaaattcc ttaactgccg tttcacaaaa aatcaaaaaa
    10741 aagcccaaca aaagcccaat caccaatcgt tatgagcaga tctgcgagaa tctttcacag
    10801 cagattgctg gacaaactcg tccattgctt gaccctcttc tttttgaaca caccttgttt
    10861 ttctgttata taccgaaaaa cggccattca gttcaaaaca aggctctaaa caaaaacagt
    10921 cgatcaccca ttcattgatg gatagcgacg attcgtttac tatttatcct gtttctacct
    10981 tttcggaaga atccggctat gtaatcaata ctgattccaa agatacactt aggaagtttg
    11041 ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt tgttgtttct gattcttttg
    11101 caatccaaga tatcaatgaa atattccaag agatcagaaa ttgcaccaaa ggacaacgtt
    11161 tctatcataa gggaaagaac cttgcttatc agcatgaact tcttccgtta ttaaccaacc
    11221 acgagcagca agcaagtagt tatttaacaa aaatcgttca taaagactac gctttagctg
    11281 tcaaagaact tgagaatttc ttcacgacga tattggaaca gcctattgat ccagcttatt
    11341 tgaaacatga ggcaatcaat cgtttctatg cattactcaa cgctttggct gacgagcatg
    11401 ttgggaatga agaactttct ttgctcaaga tcgcccttcc ttctttatta gccgatgctg
    11461 agtatttcga agatttttct attcttgtca aagaaacact tgagaaactc acccactttt
    11521 ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt gatctacgag ttcgtccagg
    11581 aaaattatga gaaagaaatc aatttacaac ttttatccga aaaatatcat ttttcatata
    11641 gctatctctc agccatcttt actgaaaaat ttggtatcaa cttttcaaaa tatctaaaaa
    11701 aagtccgtat cagtaaagcc aagctctttt tgacggaaac acggcttact ttgacggaaa
    11761 tctgtgagaa agtcggctat accgaactgg gttatttctc cagagttttt aaagaagaga
    11821 caggaatgac accttctcaa taccgtaaag gaacttttgt aaaatgagaa aaaaaatcaa
    11881 agagcatgaa ctctttgcca aacttctgtt cattttgttc atcggtcttt ttctgcaagc
    11941 catcgtcatt tctttgttta tttatcatcg atcaagggat gcctatatcc agctgttcaa
    12001 tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa tttgaaagtt tgaatgatac
    12061 aatcgagaat acattagccg cttttgattc taatccagct gtcaaaagct atttttccaa
    12121 tgatccagcc caacatatgg aacagcttca gcaactaaga acaatccaaa aaatgaatga
    12181 ctctctttca aaaatccatc caatgatcga ttacgatgta ttgatctttg gagaaaatgg
    12241 aagaactttt gtcggaaacg acatgctcac tgccgtatct gccgattctt tttttcaatc
    12301 tgcgattgct cagagagtga atgaacgtgc tgctgataca caaatgcttt ttgcacacca
    12361 cggattgact cttcgcgata agaaagcacc cagcgtcttc tttgtcaaaa aactgaaaaa
    12421 tacattgaat cacgtttacg gatatgctgt tttatcgatc tcatccaagc aactagcgaa
    12481 tctttttcaa tctgtagtaa atcctgagat atcgaagatc tccttcatga acgaagaaca
    12541 gacgatcatc gcatcaaatg aagaaaatac aattgggagc cgatcaatga ttttcaaaaa
    12601 actaacagta ggtgaaacaa cgatttcaaa tggacagcag ttgacccgtc ttcctctgta
    12661 ccgccaggat tgtacattac tcagtcaagt cgatcttcat tctttatcta accaaatggg
    12721 cgtgattttt ccgattattt tgtataatat cgtgacactt gcttttgctg gctcattggt
    12781 atttatgtat ttggatcgac acacaaaatc gatctaccgc ttgattgatt cgctaaaacg
    12841 gctagaggaa gatggcttaa aagctactgt tcctgtacaa ggaagccatg aagtccaatt
    12901 gctagggaat acgatcaatc aattgctaag taatgctcag catcattatg aacagacgat
    12961 ccaagacgaa aagaagaaac gttcgcttga aatccaaact atgcaagcac agatacagcc
    13021 ccattttatt tataatacgc tgactagctt gaaatttttg atttggcaga aaaaaacaga
    13081 tgaagcagtt caaggaatcg acagtttcat tactttacta agaagtacga ttgggaaaaa
    13141 agaagaagtg attccagtca aggaagaaat caaaagcgta caaagttaca tcgatctttt
    13201 gagtatgcga tatggcgaaa gaatctcggc taaaataatg attcctgatg aactgcagtc
    13261 attatctatg ccgaatatga tccttcaacc aattatcgaa aacgcctatc ttcatgcttt
    13321 tcacaataaa acgcaaggat tcatcacggt gtacggcaaa acaaaaaatc agcttctttt
    13381 attcgaagtg attgataacg gcgatggctt cgatactgaa aataccaaac aaaaaaatga
    13441 tcatttttca ggaatcggca tcagccatgt agatgagcgg atacgcctaa tgtatggcga
    13501 tgcatatggc ttaacgatcc aatcagtgat tgggacagga acgactgtaa cgatttgtct
    13561 accgcttctt cctaaaaatc aatcagaata tcaaaaataa agaatagcca agcgctgatt
    13621 tttttatttt aagttaaata aatcaatcag tattcttgac tttcatctac gcgtctcttc
    13681 tactgtcagc actggataac agcccaatat ttgactttct cctccgagca tcttgatttc
    13741 ttggagcgca ttctctacta atgtcattgg acggtccaac aacaaatcga tcacgaaaaa
    13801 atattctccc aagctagttt tcaatggtcg ggattcaatc ttacttaagt tgatttcccg
    13861 ccagccaaaa gctgccagca ttttatgaag catccctggc cgattagcag gtagtgtcaa
    13921 gatgacactc atttttactg gagctgactg ctggctggtc atctttcggt caccgacgat
    13981 ccaaaaacgt gtctggttca actcattatc ttggatattc tcagctaaga tctccaatcc
    14041 aacatgctgt gctgtttcta atgaagcaat cgctgctgcg tcttctttcg gatgttctgc
    14101 tacatacatt gctgcagcag ttgttgattc tgttgctact aaaggaacat taggataatg
    14161 cgtttctaaa aactgttgtg attgtgctag tgcttgtgga tgtgaaagga tttttgtaat
    14221 ctttgttgct ggattgccta aaagctgctg cttgatagga agaacgattt cacttttaac
    14281 ttcgacttct ggatgtttgg aaagaagatc gattgtgtga tgaacagctc cttccaatga
    14341 attctctaca ggaacgacag ccagatcgac ttgtttgcga aacaaagcgc gcaaacaaac
    14401 agggatggaa gcatacgcag tcaattgttc tgtaggaaat agctgacaag ccgcctgata
    14461 agtgaatgaa ctttctggcc ctagatagct gacgttcata tgcttttgat cctttctata
    14521 atggtatcga tgatcttttc tggtgtttga ttggtcgtgt tgattcgatg tgtcgcactt
    14581 tcttcatacc aagaaagtcg ttgtgccaat aatgctttta tttcttctct tgtttttcca
    14641 agagctaaag gtcgaataga cgtttcatct tgttggatac gatcaactag acagtccgct
    14701 tctgcctcta agtaaagaac aatcggctgt gtttttaaaa aacgccgatt ttctgcactt
    14761 tggacaatcc ctccaccagt cgcgataatt ttgtcttcat ttgacagctt tcttaaaata
    14821 tctgactcca cttttcgaaa gcttttttca ccgtaatagt gaaaatattc ggcaatcgac
    14881 aattggatat gttcctcaat ctttgtatct aaatctacat actctttttg taaacattga
    14941 gctaaccctt taccgattgt tgtttttcca gcgcccatga aaccaatcag caaaatgcta
    15001 gccatgagct tctcctcctg acacaagttc tgctaaatcg tcaaaaaatg ccggataaga
    15061 gattgaaaca gcttcagctc cttctagttc gcaaggtcca tcagctatca aagcagctac
    15121 ttgcagcatc ataccgattc gatgatcgcc tctgctggac acttttgcgc catgcagagg
    15181 tgtcggccca tggatgatca gaccgtctgc tgtcgctttt atctttgctc ccattttctg
    15241 taattcttct gccaccgcat caattctgtt ggtttctttt acttttaatt cttctgcgtc
    15301 tttgatgatc gtgattcctt ctgcttgagt agccactaaa gctagaatgg gcaattcatc
    15361 aatcagacgc ggaatgattt ctccaccaat cgttgctttc ttcaaatgac tcgtcttgac
    15421 acttaaatca gccgattggt tctgttcatt gtgattcgtc tgggtaatcg ctgcgcccat
    15481 ctcctccaac acatctaaga taccagttct tgttggattg accccgacat tttttaacaa
    15541 cagttggctt tccggaacaa gaagtcctgc tgctagaaag aaagcagctg atgaaatatc
    15601 acctggaaca gttatctgtt gaccagttaa tttttgcggt ccggtcacta tgatcgtttt
    15661 atcttccact gtgatccttc cgccaaattg acgaatcatt tcttccgtat ggttacggga
    15721 acgttctttt tcaacgatta cggatgtgcc ttctgcttgt aatgccgcaa acagcaaagc
    15781 agatttgact tgcgcactgg ctacaggcat attgtatcgg ataggtgata aagagcgggt
    15841 tcctgtcaca gtgattggta gatactggtc attttcttct ccttgcaagt cagcgcccat
    15901 ctcgcgtaaa ggttccatca ctcgccccat tggacgttta gacaacgaag catccccgaa
    15961 cagagtagtc gtaaaaggct gtccagccaa aatacccatc aataaacgta tggtcgtacc
    16021 ggagtttccc atgtcaattg attcggttgg ttggacaaat gagtggctac cgcgaccatg
    16081 aatcttgatc acctcttcct cttcttcgat ctcgacacct aattgttgga aggcatgtaa
    16141 agtactcaga caatcttctg cgcgcaaaaa attttgtata gtcgtcgttc cttcagcaat
    16201 tgctccaaac atgatgctgc gatgggaaat tgatttatct gctggtatcc taacagtccc
    16261 tcttaatcca tgtatttgtt gtaataattg catttatcca gcctcctatt tgatccagca
    16321 atgataactg gtattagaca taatgattct ttttgcttgt aacaaatcct gttggttttt
    16381 aaaactgatt tgcagtattc ctaaaatatc ttccctggtc tcttggatct tcaaattaat
    16441 caaagaaact cccgcttttc cgatcagtcc agttacttct gcaataacgc caggttcatc
    16501 tggcacatcg ataaataaat caaaaaaggc aggaatcgcg ccttgcttat ggacaggcaa
    16561 ctggtctcga ctttcttttg cccgattgaa aaaagaaaag atttccgatt ggttctcagt
    16621 catgatccat tctgctattt gtgtcatttc ttgttgccaa tctgccaatc gttccaataa
    16681 tatctgctta ttgctcaaca aaatatctgt ccacatgaca ggatctgagg aagcgatcct
    16741 agtgatatct cgaaagcctc ctgctgccag ttgtttggct cgcggatact cttgattgaa
    16801 ttgttctgcc tgattgacta agccagctgc cagaatatgt ggaaaatgac ttagcattcc
    16861 tgttatacgg tcatgttctc ttgctgtaag agttacatat tttgctttgg ttccgcaaaa
    16921 tagttcttgc agtttttcaa catctttttt cgtcttttcc aaaggtgtaa agatgtaata
    16981 ggcattttca aatagtttag catctgctgc ctgtatacct gatttatgag aaccagccat
    17041 tggatgtcca ccgacaaacc gaagattttt ctgctcagcg gatgtcacga cttgctgctt
    17101 cgtactgctg acatccgtaa tgatgacgtg atcagacaag ttgattcttt caaggacatc
    17161 aagataagct aaggttgtac ggacgggaac ggctaataag ataacgtctg cttgttctgc
    17221 acctgtttca aaatcacttg caacgtaatc aacgatctgc tgttctactg cttgctcacg
    17281 ggtttttgac tgactatccc aaccaactac tatcagttct ggattggtct gacgaataca
    17341 aagtgccata gaagcaccaa tcagtccaag ccccacgata aaaacatgtt gtgccatctt
    17401 atttgccccc tttttagtaa tgtttcaagt agtcacggta ctccttcact gcagctttca
    17461 actcaacaag cgaatcactt gggaattttg acaatagctc tgtcgctacc acagtggcta
    17521 caacattttc cgcaacgacg ctagctgcag gaactgctgt actatccgaa cgttctacac
    17581 ttgctttgta aggttctttt gtatcgatat caacactttg aagtggcttg tacaatgtag
    17641 gaatgggttt catgaccccg cgaatgacaa gtggttcgcc attggtcatt ccgccttcga
    17701 agccgcctag attattggta cgtcggaaat agcctctttc ttcttcccat aggatctcat
    17761 ccatcacttc tgagcctggt aatgaaccag aagtgaatcc gaccccaaat tctacgcctt
    17821 taaaggcatt gatactaacc acagcttgtg cgattttggc atctagcttc gtgtcccatt
    17881 gtacgtagct tcctaatcca gctggaacgc ctccaacgag cacttctaca attcctccga
    17941 tcgtatctcc atttttcttt gtctgatcga tcaaatcttt tattctttgc tcaactgctg
    18001 gatcaacaac tcgtacttct gacgcttcag aaatttcttt gatttcttga atcgagatat
    18061 ttccaggtat ttctgcttgg attccaccaa gacaaatcac gtgtcctgct acgatgatgt
    18121 caagttcttc cagaattttc ttggcaattg cgccgattgc tacacgcatg gtcgtctctc
    18181 ttgcagagga ccgttcaagc acattccgaa gatctcgatg atggtatttc ataccgccta
    18241 ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg cagtgattgg tctttttcag
    18301 gcacttctgc tacagacatg acagttgtcc aatttttcca atctttattt tctaccacta
    18361 aagtgacagg tgaaccaatt gttttgccgt gacggatgcc agacgtgatc cgaacacgat
    18421 ctttttcgat cttcatcctt cctcctctgc cgtaaccagc ttgtctgcga gcgagttcgt
    18481 gattgatttc ttcttctgac agaggtaatc ctgctggtag tccttcaata atggctgtta
    18541 attctggtcc atgggattct cccgcagtca tataacgcat cttacagtcc tcctaagtaa
    18601 tctttgaatg tctctgtggg aacaggaacg atttttgctt gcccaatctt ttctagcaag
    18661 atgatattca attggcttcc tcttgctttc ttatcatggg ttatcgcgtg gaataattca
    18721 tctggatgac ttagatcgta cgtaatcggc agatggaatt tttgaagtat cctttctaaa
    18781 gcagctgtcg atccagttgg agacaagccg attttttctg catgagaagt gatggctacc
    18841 attccaatag ctactccttc tccatgactg actaccccat atcctgctgt attttcaatt
    18901 gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat ttccttggtc aaagacatct
    18961 tcttccacta cttttcgttt gatttccaac gtagcagtaa tgattggaac agcatgttcc
    19021 agaagatctt tttcgtcttt cagctcgttc agctgtttcc aaagcgttgc atctgctata
    19081 gctgctgatt tgatgatttc agcgattcct tctcgtaaac gacgtaccgg caaagttttt
    19141 agaacctcag gatcaatcaa tacaccagct ggctggctaa atgttccgac aaggtttttc
    19201 gctttgtttg tattgactgc tgtcttcccg ccaatactag aatctacttg tgcaagtagc
    19261 gtagtcggaa tttgaacgaa atggatccca cgcatgaacg tcgaggccac gaatgctgct
    19321 agatcaccca ccattcctcc accaagtgca agtatcccat cgctgcgggt aaaatcatta
    19381 tctgctaaaa aatcgtataa ctcagctgct tgttctaaag atttactggt ctcacccgcc
    19441 ggtacgattg aaaggactgg ttcaaaatta gcttgtctca attggaacat gattttttct
    19501 gcatatagtg gagcaacgtt tgtatcagtg atcacagcta ctttttgagg tctccaaatc
    19561 gtttgaagcc atgatccagc ctgttctagt gcgccttttt caatatgaat atcgtatttt
    19621 tggttatcaa catgtacagt aagcattcgt ttcacttctt tcttttaaaa agctggaatt
    19681 aatcgagtgt ctgaatcttt tccattgctt ctttcatgat cgcaacttct ttcatcatgc
    19741 gtgagtaagt cttttcattc aacgattggg gaccgtctga ccaagcattt gcaggatcag
    19801 gatggatctc tacgatcatg ccatctgctc cgcttgccac acccgcacga gccattggtg
    19861 gtacaagttc ccaaattcct gtaccatgac ttggatcaac aatcacaggg aaatgagtta
    19921 attttttcat caatgggaca gcacttaaat caaacgtatt acgtgtcgct gtttcgtaag
    19981 tccgaatccc acgttcgata aaaatgacag gagatttgtc ttcgactgca atgtattctg
    20041 cagcattaag ccattcattg atcgtaccgg aaattccacg tttcaatcca acaggtttac
    20101 ctgttttacc gacagcagat agcagtttaa aattttgcat attacgtgcg ccaatctgta
    20161 ggatatcgct gtactcagcc accatatcga tatgcccttc atccatcact tcagtaatca
    20221 ctttcattcc aaattcatca gccgcttgac ggatgtattt caatccttct tcttctaaac
    20281 cttggaaagc atatggagaa gtacgtggtt tgaaggcacc acctcgcaga atttttgcgc
    20341 ccccggcttt ggccattcgc gcacattctc tgatttggtc taatccttcg atagaacaag
    20401 gaccagccat cgtcacgaaa ctgccgtcac caatttttac tccgtctaca tccacgactg
    20461 tattgccagg atggaattct cgagaagtca atttgtatgt gtttaaaata cgcacagcat
    20521 tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa acttcgcgta tcacctttta
    20581 gtccaatcac tactcgttct gttccttgat cgattgagac tttgaatcct tctttttcga
    20641 tatgatcgac gacttttttt atttgttctt ccgttgcttc ttttttcata attactatca
    20701 ttgtttttcc cgctttcttt tgtttactta tctatcttgg ctgattttct ctactttttc
    20761 aaggtggttt tacattaatt cctactaaat gaccgtgtcc tgttgttctc attttcgctc
    20821 cttctttcct acttatgacc cagataatca acaaaaaaag atgtccctgt aggaaattgc
    20881 ctctacagtg aacatcttca acgagcctta aaaagcgctg caaaaatata aaatagccac
    20941 tatagatgaa tttctctacg tggctgtaag tcgtgtttaa aaatctttag ccatcataga
    21001 tacaaacctg gtttgtttgt acccacgaag tcatgaatac aaacctatct aaaatagcta
    21061 aagtaatacg tattcagttt tgtaagtttt ctagatttca taaaaaatcg ctccaaactg
    21121 atgtaagaat tgtacctagt atagacataa aaaaatcgac tgtcaatcat ttttatgaaa
    21181 ctttttttaa ttttctgata gttaactttg tttttcagaa aaataacaat tggttttatt
    21241 gaagaaaata gtcatgaaca aaagtatctg agaacttttt tactctcttg ttcatgacat
    21301 atagaataaa gcagctttgt cttgtaaact ccgtttttca aataataatc taaagattgc
    21361 tactgaataa taaagccgat ttcatggtcc tcttcgctgc tgattcggta aggtgactca
    21421 acatccgctc cccagctatt gatacccccg actcctctta cttttgcaaa aatcgtaaga
    21481 accgttcgtc tttctggcgg tagttcttct atatgtgttg cctgttccag ttcttctgct
    21541 ttgaatggta aagcggaaaa agcaaatggc gtatcctgct gttcaaatcg caacgaaaaa
    21601 tcttcttgat ctttatccgt attatccaac gtttgatttc ttgttagttc tacccatttc
    21661 gtttccatat gcatgccgca ttcttgggga acaaggtatg gggaaacggg gagtcctgcc
    21721 actttataaa taccttcgac accaccagcc atccgatccg gatatgtttc ccctgatagg
    21781 ccttcatact caaaaaattt tgctttcgtt ggcataatga accttaaacc caatgcgggt
    21841 aattccggta atcctttttg tccataataa tgcatattga caagcatttt accctctcca
    21901 gcgatttggt aggaaattgt cacttgggtt tttggtactg tgttgatttc cagtgtaaaa
    21961 atgatttcaa ccgtttcagc gtattcatta cttgaatatt gattatttac tggagcaatc
    22021 ggcaaatcga tctttttatt gtccaccagt acttctattt ctttgacttt tggatacaat
    22081 ccggctccta accacatacc ggatttaaat ccaaatccgc tacctctgtc attatcggtc
    22141 gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc attcttttcc gtttttattg
    22201 agcgattcca gtccgcctct tgtataagaa aaaatataat gcgtattccc cgatttgaca
    22261 cctaatgtac agtcgccgaa gacgattgca actttttccg tatccatatg acttttattt
    22321 actgtatttt tcataataat atcttacctc ctgcatggcg ggtttttctt gtccatctgc
    22381 aaaaacgatc ccattccctg agaattcata gtcacttggt ttatcgtcaa agtcaccgcc
    22441 atagcgcatc acttctctgc ctgtgattgg atcagtcact tggatcgcct gatctttaaa
    22501 atcccatata tagcctcctt ggtacatttc ataacgatca agcaacgtca tgtaagaatc
    22561 cattccacct aacgaattcc ccatgtcatg catatattca cataagatga acggtttttc
    22621 cgctttattt tcaaggtagt caatgatctc ttctggtggc gcatacatgc agctttttac
    22681 atctgaaatc gaatcttcga atgctggatt tggaaaattc ccttcataat ggacaagtcg
    22741 atcggggtct acttttttga agtaatcgtt catttgtcga atgttttctt ccgcataaga
    22801 ttcatttcca agagaccaaa aaagaatact tggatggttt tttaatgttt cgaaattcgt
    22861 ttttgctcga tcaagaacag ccgcctgcca ctgaggtact gcacctggta cgttgtaaga
    22921 aggctcaaat gcgcccattt tttgccatga gccatgggat tcgaggttcg tttccgccat
    22981 cacatggatt cctgcttggt cgcataaata ataccaagtg atctggttgg ggtaatgaga
    23041 tgtccgtacg ccgttgatct ttgcattttt tatgatagcc aaatcttttt ccatatcttc
    23101 tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt cgatttaccc cgttgataat
    23161 catccgtttt ccattgaagt agatgatttt atctttgatt tcgattttac gaaaaccgaa
    23221 ggaatagtca atatactccg ttactctttt ttcttcgtct atcgtctcca attcaagctt
    23281 atataaattg ggctgaaaat gcccccacag tgccacattt tcaaatccat cgatgcgaat
    23341 cgtcatttgc tcttttaacg gatattgctg ttgatagatt tccttcccat caggatctag
    23401 tatcgctaca ttcagctgac tgtttttggc tacttgctca aaagacaaat ctacttcaag
    23461 tttgccattt tgacatttgt catctaagcg aggtttgatc caaagatctt ctaaatggtt
    23521 ttgtcctcga gcaactaaga agacatctct gaagatccca aaaaatcgga aaaagtcctg
    23581 atcttccaag aatgcagctg tacttctttt gtaaacctcc acagctagaa tattttcttc
    23641 ttccaaataa ggtgttaaat caaattcaga cggagtgaac gaatcttcgg catacccgat
    23701 aaaatgacca ttcaaccaaa taaacattgc ttgttctaca ccagcaaaat gaatcgagat
    23761 ttgttttccc tttagacctt cttctaatcg aaaagtcgtc aaatacgaac caaccggatt
    23821 gtctttagca gctgaaaaga tcccttccgc ctctttctca gggcttgtcg tgaaaggggg
    23881 acggcgaaat acatgtccct cccacggata catcgtattg atgtaatgga tcttgtcata
    23941 atttgctaat tcaatatgac taggaacagt gatttcatca aaagaacagg catcaaaatc
    24001 ttttttataa aatgtttttg gtcgtaccga aggatttggc gaataacaaa aacgccatga
    24061 tccatttaaa gactgtttaa gggaggatgt ggtttctact tcttcccttg ttgcataaca
    24121 aacatgatcg ctatgtgcat ttgattggtt cactctaaaa acttgtggat catctaacca
    24181 ttttatattt gctttcatac ttttttcctt ctctctcata actttttgaa atgaagcagt
    24241 tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc caactgtcat caattcggcc
    24301 cccgaaaata tccgcttcga ggtctgttcc tgataaagtg cctcatcatc taaccccatc
    24361 agatatactt catggaacgc tggttgagct gaagctagta tcctccctaa aaacacaata
    24421 gcttcatctt gtttaggaga cacaaacatc catgcggcaa tattttcttc aaaaggactc
    24481 gccaaacgat aataatggcc atactggatc accggacgaa tcgtttgata ggatataatt
    24541 tggtctttta cttgcttttt ttcttcagga gacagctttg tcaaatccag ttcataacca
    24601 aaaacagcgc tcatagctgt ttctcctctt gttttcaacg aagtagatct gtcggtctga
    24661 tgatttggta cagctgatac atgagctgtg atggacgaaa tcggataagc aaggcttgtt
    24721 ccatattgga ttttcatacg ctccactgca tccgtgttat cacttgtcca tgactgcggc
    24781 atataataaa taaacccggc atcaaaccgt ccacctccac cagaacagcc ttcccataag
    24841 atttccggat aatcagttgt caaatgttca agtagttcat aaagcccgag catataacga
    24901 tgcgctactt ctccttgtct ctgtgggtca agggtaatcg aatagacatc tgaaagcgaa
    24961 cggttcatat cccatttgat gtagtcaaga ggaatcgtat ctaaaatctt tctcatttgt
    25021 ttctcaatcg tctgccgaac atctaacctt gtaaaatcca gtacatgttg tgatctggaa
    25081 gcagaaggca ttctacctgg ctcctgcata agaaaatcag gatgtgttcg atacagttca
    25141 gaatcaaccg agatcatttc aggttcaaac cataaaccaa atttcaatcc tttgctatga
    25201 gcataatctg ctatttcacg taatccattt gtcaacttgc cttcatactc gaaccagtct
    25261 cctaatgaag aattgtccgc atctcttttt ccaaaccagc catcatctaa tacgaacatt
    25321 tcgatgccta gctcctttgc ttcatcaatg atcgccttga ttttttcact attgaagtca
    25381 aagtaagtgg cttcccagtt attgaccaga atcggccgtt cagcaaattg atgacgacct
    25441 cttgcgattc tttcacgtag caactcatgg tggatctgac tcattttgtt caatccctga
    25501 cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact ggcctgcatt caattcccac
    25561 gaaaaacgat ggctgttgat cccaccgacg actctcaatt gattgatctg atctttttca
    25621 agttcgaaag aatggttgcc ggaataaaca aattgaagtc cgatcgcttc tccagtattt
    25681 tctgtcgtat gatgatgaac taaagcaata aagttattca tatggtgact gctcgttcct
    25741 cgacggcttt caaatacttt tgttccagat aaaacagatt gccgtgaaat ctgtctttca
    25801 ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc gcctagtatg ggcaaagtct
    25861 agctggaaac tagccgcttt ttctagtttg attgtttctc cggtttcatt tcttatcttt
    25921 acagatcgtg tgatcactgc tctatcagta aatatggtat agaacaaatc gaaataaatc
    25981 gctgtttctc tatccttaag tgaaatcacc aacgtttgtg cctctgactt gtccatcaca
    26041 taagtttgag gcaagccttc gatctctggc ttcccatctg taatataatg gctgtcatat
    26101 ctcagatcca gtaagtctga tccattcttg cgctgaatga tcgatgcggg cgttcgaaag
    26161 tccatgctgc catgagaaga gtattcttgc ggtagtgtat ctttcgacag agaccggtca
    26221 ggatttaagg gaacatttcc tgaaaatcca cgatctcttc ggggataatt tttattgtct
    26281 ttgtactgct tgactggttt gccaaaataa atatgcgcca atacattgct ttcttctatc
    26341 tttatcagat aagaaagaaa tgtatttcgt aaatggaaga ctcggctctt ttcatcaaat
    26401 aagatgaaat ttttatctgt ttttctatgc gtaatatcca tcatttcact cccaacaaaa
    26461 cggaaaccgt ttactttttt ttataattcc tgtatactgt ttactaaaca attttgtcaa
    26521 taaaaatttt ttattttttt gaggagtgtg taaaaatggt tggaattaga gatgtcgcac
    26581 gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt gaataacgat cagacgatcc
    26641 atgtggctca gactactcgg caacggatag tagaggcagc tgctgcgttg gattatgaca
    26701 tcagtactcg taaatatatc aaaaagagaa tgccttcgat tggtgtgatc tctacgatcg
    26761 gcaaagcgag cgaagaacag gatatctatt acaaacattt gcgagtaggg atggaagaag
    26821 aagctcgacg tctgcattta gggatgaatc gaatctataa tctatctgat aatcctaaac
    26881 aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt tgggactgtt acgaaagaat
    26941 cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt cgtggataac cctgacatcc
    27001 agcaagaggt cgatatggtg tatgcagatc ttgaacggat gactaaaacc gtactggaat
    27061 tgttcttaga aaatggtcat aaaagaatag cctatatcgg gggatataaa gtcgatatgg
    27121 atgaattcgg tgggaagcag atttctataa atgaaaagag gatgcgtgcc tatcagcatt
    27181 ttatgatcga tcatcactta gaaaaatata tgtcttatca tctaggtggc tggacagaaa
    27241 aacaaggtga ggtacttgcg actaatctat tagaaaatga atcccctctt cctactgcca
    27301 ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg ggcattgaaa caaaaaaata
    27361 tccaaattgg agaagagatt gttgtagcaa gctttgataa tatcgagaca atcgcaaacc
    27421 tctcccctgg cttgaccagt gttcaaatca atgcgaaatc aattgggcaa gcagctgttc
    27481 gaatggcatt ggagcgaatt gacggtatac gggacgaacc aatcattttg acttatcctg
    27541 cacgattgat tgttagggaa agttttatcc caagaacaaa aaggtattga aagagaggtt
    27601 gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg gctttttcac aacctcttac
    27661 ttatacttat tcacaaaatc caatgtctgc tggtaacaat tttgcgattc ttttagcgtg
    27721 taatcaaatt ggtattcatg ggtaatctct ttttttgtat ctgtataaaa caaggattgc
    27781 acgggtacct ttaattctgt caaccgattt tctaatgctt gaccttgctc ctggaatgag
    27841 tatgcatttc cgtcagttat atacgttggc ggaaattcct tagaaacatg gtcagccaca
    27901 ctagcttctt tcaattgctc actcgttttc caatcttttg ttccaatcaa cgaccacgca
    27961 acggttttga cgaaaaactt cataaatcga tcatccgaat gctgctgtgc tgttagcttc
    28021 aaatcaacag gtccgcaata agaaatggtt cctttcagac tatcttttgg aagtagctgt
    28081 ttgattttca tttgtttagc atacgcatca ttcgtttgta tagctgcata ttgcaatgcg
    28141 atttgtgaac ctgcactatc tccaccaaaa aataattgtt ccatgtctag caatttgtcg
    28201 tctttttctt cagctaataa atctttgatc agttcattca cttgaagaac ttgattaggg
    28261 tattctgaat caggcgctaa ttcataattc atcgcaacca ctgcgatgtt ggcgtctgaa
    28321 actaattttg tcgcaaattc tttgacaccc gatttatctc cgccgacgaa tcctccacca
    28381 tgtacccaaa atagaacagg aacaggcccc ttgctgtttt ttggatagta gatgtcataa
    28441 gtattctctt tatacttgga ctcatacgtc aaatctgttt ttttcgccac gtttttttct
    28501 gcttgatcga acgttttttt atcagtgatt tgaacagtat ttgaaaacat atggttgatc
    28561 aagaatgctc caggtctggg ggaaagctgg aaggcaagaa agattgcaaa cgcgatccca
    28621 agaatgattc cgaagaacca tccgataaat ttaagaattt tctttttcat aaggctccct
    28681 cagtagttag tattaagcat tctgattata ccatagtgtt atttcctatc aagctctcct
    28741 agatgattta atgattttcc ccaaacttct tctgcaatgc ttttgacaaa agaaattttt
    28801 tccattgttc ttcaaacaaa acattttctt cttctgttga agcaaatcca cattgaggac
    28861 ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc gtagatccgg tcaatcactt
    28921 gctggtgctc ttccatttcg ccatttttcg ttacagtcaa aatctaagac aactaattta
    28981 tcttcagaga ctttctctaa aagaacaaaa ctcccagcac gttcactatc gtattctaaa
    29041 taataggcac ggacattttc ttgagtaaaa taatgggtct gctacgtcgt catcccccct
    29101 gatgccgcca aaatcgaata ataatttccg cgtcaaacat gtatatttag tgttgaatca
    29161 tttgagaata tgaaaataaa aatctttttg atcaatcagt taatcaaatt gaaataacga
    29221 aaaatctgag acagaatgac gttcattctg acccagattt gattttgcac aactcaacag
    29281 tccttattgg caaatcgcca aaataatgta gactaactaa a
//
LOCUS       DACECB010000031        29105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECB010000031 DACECB010000000
VERSION     DACECB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.031"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1028)
                     /locus_tag="IWO04_001448"
     CDS             complement(<1..1028)
                     /locus_tag="IWO04_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0213068.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="IWO04_001449"
     CDS             complement(1110..1799)
                     /locus_tag="IWO04_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0213069.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IWO04_001450"
     CDS             complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IWO04_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0213070.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2326..2829)
                     /locus_tag="IWO04_001451"
     CDS             complement(2326..2829)
                     /locus_tag="IWO04_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0213071.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IWO04_001452"
     CDS             complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IWO04_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0213072.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IWO04_001453"
     CDS             complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IWO04_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0213073.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="IWO04_001454"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="IWO04_001454"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0213074.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="IWO04_001455"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="IWO04_001455"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0213075.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="IWO04_001456"
     CDS             complement(6441..7460)
                     /locus_tag="IWO04_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0213076.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8520)
                     /locus_tag="IWO04_001457"
     CDS             complement(7633..8520)
                     /locus_tag="IWO04_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0213077.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IWO04_001458"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IWO04_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0213078.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IWO04_001459"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IWO04_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0213079.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IWO04_001460"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IWO04_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0213080.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="IWO04_001461"
     CDS             9751..10395
                     /locus_tag="IWO04_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0213081.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="IWO04_001462"
     CDS             complement(10451..10954)
                     /locus_tag="IWO04_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0213082.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="IWO04_001463"
     CDS             11551..12516
                     /locus_tag="IWO04_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0213083.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="IWO04_001464"
     CDS             complement(12615..13787)
                     /locus_tag="IWO04_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213084.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="IWO04_001465"
     CDS             complement(13774..14919)
                     /locus_tag="IWO04_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213085.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="IWO04_001466"
     CDS             complement(14933..15865)
                     /locus_tag="IWO04_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213086.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="IWO04_001467"
     CDS             15982..17061
                     /locus_tag="IWO04_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0213087.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="IWO04_001468"
     CDS             17058..17687
                     /locus_tag="IWO04_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213088.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="IWO04_001469"
     CDS             complement(17797..18024)
                     /locus_tag="IWO04_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0213089.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="IWO04_001470"
     CDS             complement(18127..20715)
                     /locus_tag="IWO04_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0213090.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="IWO04_001471"
     CDS             complement(20735..21394)
                     /locus_tag="IWO04_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0213091.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IWO04_001472"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IWO04_001472"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0213092.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="IWO04_001473"
     CDS             complement(22167..23033)
                     /locus_tag="IWO04_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0213093.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="IWO04_001474"
     CDS             23142..23783
                     /locus_tag="IWO04_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0213094.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IWO04_001475"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IWO04_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0213095.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="IWO04_001476"
     CDS             complement(25224..26123)
                     /locus_tag="IWO04_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0213096.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="IWO04_001477"
     CDS             complement(26125..26922)
                     /locus_tag="IWO04_001477"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0213097.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="IWO04_001478"
     CDS             complement(26900..27604)
                     /locus_tag="IWO04_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0213098.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="IWO04_001479"
     CDS             27735..28310
                     /locus_tag="IWO04_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0213099.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..29105
                     /locus_tag="IWO04_001480"
     CDS             28428..29105
                     /locus_tag="IWO04_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0213100.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8562 a         6169 c         5232 g         9142 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     1921 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     1981 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2041 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2101 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2161 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2221 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2281 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2341 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2401 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2461 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2521 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2581 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2641 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2701 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2761 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     2821 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     2881 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     2941 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3001 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3061 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3121 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3181 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3241 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3301 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3361 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3421 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3481 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3541 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3601 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3661 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3721 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3781 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     3841 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     3901 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     3961 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4021 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4081 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4141 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4201 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4261 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4321 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4381 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4441 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4501 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4561 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 attaa
//
LOCUS       DACECB010000032        28030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECB010000032 DACECB010000000
VERSION     DACECB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.032"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWO04_001481"
     CDS             185..1183
                     /locus_tag="IWO04_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0213101.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWO04_001482"
     CDS             1295..1804
                     /locus_tag="IWO04_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0213102.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWO04_001483"
     CDS             complement(1834..2493)
                     /locus_tag="IWO04_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0213103.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWO04_001484"
     CDS             complement(2495..3259)
                     /locus_tag="IWO04_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0213104.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWO04_001485"
     CDS             complement(3267..3944)
                     /locus_tag="IWO04_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0213105.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWO04_001486"
     CDS             complement(3981..5366)
                     /locus_tag="IWO04_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0213106.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWO04_001487"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO04_001487"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO04_001488"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWO04_001488"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0213107.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWO04_001489"
     CDS             6305..7162
                     /locus_tag="IWO04_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0213108.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWO04_001490"
     CDS             complement(7243..7578)
                     /locus_tag="IWO04_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213109.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWO04_001491"
     CDS             complement(7645..9015)
                     /locus_tag="IWO04_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0213110.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWO04_001492"
     CDS             complement(9093..9629)
                     /locus_tag="IWO04_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0213111.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IWO04_001493"
     CDS             9938..11419
                     /locus_tag="IWO04_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0213112.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IWO04_001494"
     CDS             complement(11522..12229)
                     /locus_tag="IWO04_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0213113.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IWO04_001495"
     CDS             complement(12308..12646)
                     /locus_tag="IWO04_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0213114.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IWO04_001496"
     CDS             complement(12757..13719)
                     /locus_tag="IWO04_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0213115.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IWO04_001497"
     CDS             complement(13734..15065)
                     /locus_tag="IWO04_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0213116.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IWO04_001498"
     CDS             complement(15073..15753)
                     /locus_tag="IWO04_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0213117.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IWO04_001499"
     CDS             complement(15972..16451)
                     /locus_tag="IWO04_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0213118.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IWO04_001500"
     CDS             complement(16578..18257)
                     /locus_tag="IWO04_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0213119.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IWO04_001501"
     CDS             18805..20307
                     /locus_tag="IWO04_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213120.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IWO04_001502"
     CDS             20320..20982
                     /locus_tag="IWO04_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213121.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IWO04_001503"
     CDS             complement(21083..21286)
                     /locus_tag="IWO04_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0213122.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWO04_001504"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWO04_001504"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0213123.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWO04_001505"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWO04_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0213124.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(25250..25864)
                     /locus_tag="IWO04_001506"
     CDS             complement(25250..25864)
                     /locus_tag="IWO04_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0213125.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(26470..26847)
                     /locus_tag="IWO04_001507"
     CDS             complement(26470..26847)
                     /locus_tag="IWO04_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213126.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWO04_001508"
     CDS             complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWO04_001508"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0213127.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         8325 a         5873 c         4685 g         9147 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat
    24541 tatacagtaa aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt
    24601 ttgttttttt tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt
    24661 atttctctgt ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct
    24721 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
    24781 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
    24841 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
    24901 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
    24961 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
    25021 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
    25081 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
    25141 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
    25201 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
    25261 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
    25321 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
    25381 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
    25441 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
    25501 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
    25561 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
    25621 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
    25681 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
    25741 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
    25801 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
    25861 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
    25921 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
    25981 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
    26041 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
    26101 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
    26161 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
    26221 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
    26281 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
    26341 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
    26401 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
    26461 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
    26521 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
    26581 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
    26641 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
    26701 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
    26761 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
    26821 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
    26881 ttcgcttttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
    26941 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
    27001 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
    27061 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
    27121 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
    27181 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
    27241 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
    27301 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
    27361 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
    27421 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
    27481 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
    27541 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
    27601 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
    27661 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
    27721 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
    27781 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
    27841 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
    27901 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
    27961 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
    28021 accttcactt
//
LOCUS       DACECB010000033        27718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECB010000033 DACECB010000000
VERSION     DACECB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.033"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..520)
                     /locus_tag="IWO04_001509"
     CDS             complement(<1..520)
                     /locus_tag="IWO04_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0213128.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQ"
     gene            515..781
                     /locus_tag="IWO04_001510"
     CDS             515..781
                     /locus_tag="IWO04_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0213129.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(957..1526)
                     /locus_tag="IWO04_001511"
     CDS             complement(957..1526)
                     /locus_tag="IWO04_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0213130.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1600..2889)
                     /locus_tag="IWO04_001512"
     CDS             complement(1600..2889)
                     /locus_tag="IWO04_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213131.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3243..4091
                     /locus_tag="IWO04_001513"
     CDS             3243..4091
                     /locus_tag="IWO04_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0213132.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4236..5177
                     /locus_tag="IWO04_001514"
     CDS             4236..5177
                     /locus_tag="IWO04_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0213133.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5167..7338
                     /locus_tag="IWO04_001515"
                     /pseudo
     CDS             5167..7338
                     /locus_tag="IWO04_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7450..9621)
                     /gene="copB"
                     /locus_tag="IWO04_001516"
     CDS             complement(7450..9621)
                     /gene="copB"
                     /locus_tag="IWO04_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0213134.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9641..11827)
                     /locus_tag="IWO04_001517"
     CDS             complement(9641..11827)
                     /locus_tag="IWO04_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0213135.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11827..12036)
                     /gene="copZ"
                     /locus_tag="IWO04_001518"
     CDS             complement(11827..12036)
                     /gene="copZ"
                     /locus_tag="IWO04_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0213136.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12049..12489)
                     /locus_tag="IWO04_001519"
     CDS             complement(12049..12489)
                     /locus_tag="IWO04_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0213137.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12563..13102)
                     /locus_tag="IWO04_001520"
     CDS             complement(12563..13102)
                     /locus_tag="IWO04_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0213138.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13717..15183)
                     /locus_tag="IWO04_001521"
     CDS             complement(13717..15183)
                     /locus_tag="IWO04_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0213139.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15494..17575)
                     /gene="cadA"
                     /locus_tag="IWO04_001522"
     CDS             complement(15494..17575)
                     /gene="cadA"
                     /locus_tag="IWO04_001522"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0213140.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(18099..18458)
                     /locus_tag="IWO04_001523"
     CDS             complement(18099..18458)
                     /locus_tag="IWO04_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0213141.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18488..18829)
                     /locus_tag="IWO04_001524"
     CDS             complement(18488..18829)
                     /locus_tag="IWO04_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213142.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(18826..19500)
                     /locus_tag="IWO04_001525"
     CDS             complement(18826..19500)
                     /locus_tag="IWO04_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0213143.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20553..21851)
                     /gene="asnS"
                     /locus_tag="IWO04_001526"
     CDS             complement(20553..21851)
                     /gene="asnS"
                     /locus_tag="IWO04_001526"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0213144.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21891..23081)
                     /locus_tag="IWO04_001527"
     CDS             complement(21891..23081)
                     /locus_tag="IWO04_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0213145.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23102..23629)
                     /locus_tag="IWO04_001528"
     CDS             complement(23102..23629)
                     /locus_tag="IWO04_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0213146.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23676..26465)
                     /locus_tag="IWO04_001529"
     CDS             complement(23676..26465)
                     /locus_tag="IWO04_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0213147.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26612..26812)
                     /locus_tag="IWO04_001530"
     CDS             complement(26612..26812)
                     /locus_tag="IWO04_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0213148.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27264..27584)
                     /locus_tag="IWO04_001531"
     CDS             complement(27264..27584)
                     /locus_tag="IWO04_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213149.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7789 a         5884 c         4868 g         9177 t
ORIGIN      
        1 cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt tcttttccag
       61 tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc catccagcta
      121 catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa tttgttccat
      181 ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt ttatcctccg
      241 aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt ttttctttac
      301 ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa atattttgtg
      361 ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat gttaatggaa
      421 taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag ttcttttcaa
      481 aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg gctcacgagg
      541 ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa cattatgttt
      601 tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca aagatttttt
      661 aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg aagtgaaaaa
      721 aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc actcaaattg
      781 agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat aagcgaacat
      841 gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg cgttcgttca
      901 attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg gttttcttat
      961 agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga ttgactgttt
     1021 tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct taactggtag
     1081 ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc aaatccggtc
     1141 tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc tgtaatcgtc
     1201 acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc ttcagcattg
     1261 gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg ttctttcctc
     1321 tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt gtattgttct
     1381 tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc agttcccgcg
     1441 tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat cgaagatgtg
     1501 ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc tttttattat
     1561 agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt tgcctaacca
     1621 gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca gatacgtttt
     1681 ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat ctgcataccg
     1741 aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc ttagcgtttt
     1801 gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa aaaagacgat
     1861 tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt tcagtaaaag
     1921 catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc gaaattgata
     1981 tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac gaacttcttc
     2041 tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa gtacttttat
     2101 cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg aatcaagctt
     2161 ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt tccaccgctt
     2221 ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt cttttattgg
     2281 caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata gtgttcgact
     2341 gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt ggatgaacat
     2401 ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt tcaaaaagta
     2461 aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt tataagaaaa
     2521 agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac gatcgtactt
     2581 ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg gtgattggat
     2641 cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta actgaacggt
     2701 tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt tcagatgccg
     2761 atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc gccagatcaa
     2821 atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata atagtttttg
     2881 ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca aaatgagttc
     2941 actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat cttcctcatt
     3001 attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta ctttaaaaaa
     3061 acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg aaataaatag
     3121 cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc aaacaaaaaa
     3181 tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca actatccaat
     3241 tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt cccggtgtag
     3301 gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc agcagagtcc
     3361 aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac aattcacaat
     3421 caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg gcagatgaga
     3481 tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc gagaatcatg
     3541 caacgatcga caacaataca taccctctag acaatcattt ttttgtctta gctacgcaaa
     3601 atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat cgttttttat
     3661 tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt ttggataaat
     3721 tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata gaagaaataa
     3781 aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc ttgcagttag
     3841 tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga ggaagccttt
     3901 cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac gtcacgccca
     3961 aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt tacgatcgtt
     4021 cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa attcctattc
     4081 ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat taattttgtg
     4141 ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa ttctttaggc
     4201 tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc attaagcagc
     4261 tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg caaaaaaagt
     4321 atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct ccctcgttta
     4381 caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt cttacaaata
     4441 aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat gaaacattaa
     4501 ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca aaaagagaga
     4561 tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa aaattacaga
     4621 gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat tttcgggaac
     4681 atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg tctgctcata
     4741 ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct cctctttttt
     4801 gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat ttttctttat
     4861 ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc gcctttcatg
     4921 gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag aaagcttttt
     4981 tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc
     5041 ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat cttgtttatt
     5101 tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg ccaggaggtg
     5161 aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt tgtgatacta
     5221 agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta ccttcttatc
     5281 ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg
     5341 ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc gaagacattt
     5401 tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt ccatctatcc
     5461 caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat gttgctgatc
     5521 tatttattta ttacattgac cgtggattac gactattggt acgcaccctt tctttctttg
     5581 atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta cgaaatgatt
     5641 gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc catccggttg
     5701 ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg tttcttaact
     5761 tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga ggaaacgagt
     5821 gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca tgagtatcaa
     5881 ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg tggaccttta
     5941 tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta ttggaagatc
     6001 gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc acccatccaa
     6061 ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga agacaaagaa
     6121 aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg aaatactacc
     6181 ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga aagccagcga
     6241 ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga aaggttagtt
     6301 tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga aaatcagcag
     6361 acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc ggtcgaattg
     6421 accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta tttaaaaaca
     6481 agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga acacgattat
     6541 gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc aacttctatg
     6601 gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt tacaaatggg
     6661 ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa tagtcatgct
     6721 cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga accaacccct
     6781 tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac cgtccagcct
     6841 gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag tgagagatct
     6901 gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt cttattcagc
     6961 cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa atctctgcgc
     7021 tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat tgcatatcaa
     7081 aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa
     7141 tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg attaactact
     7201 caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga taaaaaaacc
     7261 tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct gacaaaaggg
     7321 agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat gccgagaacc
     7381 agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt
     7441 ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta ccgtacttaa
     7501 cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg caagaattcc
     7561 ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt tctggatcat
     7621 tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt
     7681 tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga ttgccatccc
     7741 gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca tgattacttt
     7801 tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta ataaaccgcc
     7861 ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt tattgtcacc
     7921 agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt cttttgcttc
     7981 tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta aaatcaggaa
     8041 gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta cgtttaatcc
     8101 gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga ctgttgcctc
     8161 tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga tttcatgttc
     8221 tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt ccaaggctcc
     8281 tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga cacccgttac
     8341 agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat ctagatcatt
     8401 ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg ctattgatgt
     8461 ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg cgatgataaa
     8521 gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc aggcgatgaa
     8581 tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt tgtcagataa
     8641 tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa ccttcgctag
     8701 ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc cattgataga
     8761 accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc cagtcacagc
     8821 tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca ttttatcacc
     8881 agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg tctcttcttt
     8941 tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt tttgtaaagc
     9001 attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca taataacgat
     9061 caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga gcagatttgc
     9121 gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga tcagtgtcat
     9181 catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg gctgaccgcc
     9241 atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag gaaacgttac
     9301 ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga tagctaggat
     9361 aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc
     9421 gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta tatctccatg
     9481 gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca tctccatctg
     9541 tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct catgtttttt
     9601 ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc atgtgtcgat
     9661 ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct cctcctgcaa
     9721 tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta tagacaaaag
     9781 cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg attgtttgtg
     9841 caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg
     9901 ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca tcattgattc
     9961 catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg taggctgctt
    10021 tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact tgtttaccaa
    10081 tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat cctttattct
    10141 ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg gcaatccacc
    10201 cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt tctaattcta
    10261 acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt ttacgtgtac
    10321 ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg tgggctgtaa
    10381 aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg attgctttac
    10441 ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact tcttttgaac
    10501 cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa
    10561 tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa attccatttc
    10621 gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct aatgcacatg
    10681 gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa tcaccagtga
    10741 tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga acaaaaatcc
    10801 ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca tcttccacca
    10861 tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc atgtgtagca
    10921 gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt tctacaggaa
    10981 gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata attcgaccat
    11041 ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact acttcttcaa
    11101 tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt tgaagagaca
    11161 tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa tatttgccga
    11221 gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg gggtgtccac
    11281 ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt gctaccaata
    11341 catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa aaacgagcac
    11401 cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa tgaaaaaagt
    11461 gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc aacggcaaag
    11521 tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct tgcttttctt
    11581 ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg atgttttcta
    11641 cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct ttttcagtgg
    11701 ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt tcgatcctag
    11761 cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg gtttctgctg
    11821 ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac aaatcttttc
    11881 ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca aatttacttt
    11941 cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg cacaatgatt
    12001 gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct attgatgttg
    12061 tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg gttgcttttg
    12121 ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt cttctactag
    12181 atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat tttccgtcgc
    12241 actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt ttccttcttg
    12301 ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga tcgttgctgg
    12361 tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg tctgtccaag
    12421 tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg gcgttttgac
    12481 tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta tgaatacaga
    12541 ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat ttagttaaaa
    12601 attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca ttagctactt
    12661 ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata aaagataaaa
    12721 attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct ggccgagatt
    12781 ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg agataacgcg
    12841 gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat gtagaagtat
    12901 aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt tcttgcaaaa
    12961 accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct agcggcagta
    13021 gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc gacccacttt
    13081 gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt ggttcgatca
    13141 taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct
    13201 tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa aaacggcaaa
    13261 atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg ctgattttac
    13321 aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca caatagtcaa
    13381 caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa acggcaggct
    13441 cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccagcaaa
    13501 acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct
    13561 ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa caagccgaaa
    13621 cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
    13681 cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg
    13741 ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa
    13801 gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg ctttaggatc
    13861 agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa
    13921 gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt
    13981 gaatttctta taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat
    14041 tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca tatctttgat
    14101 accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt
    14161 caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc
    14221 atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc
    14281 aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc
    14341 taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcgct
    14401 agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc
    14461 taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg
    14521 ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg tgaaataact
    14581 gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat
    14641 cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa
    14701 catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa
    14761 atataaaaag gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt
    14821 atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga ttgcttgagg
    14881 cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag
    14941 tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa
    15001 ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa
    15061 tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat
    15121 gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt
    15181 cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt
    15241 aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt
    15301 ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt taccttattg
    15361 aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta
    15421 tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg
    15481 tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca ttcaaaacag
    15541 ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca aaaccaaaag
    15601 cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc tgtttgacga
    15661 ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt tgatcattca
    15721 tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc attgcgattc
    15781 ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca aatgcggtct
    15841 ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct ggaagcaatt
    15901 cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt tttttcgaat
    15961 cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc aatgcgtcta
    16021 cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt tgatcgatcg
    16081 ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca aatctttcca
    16141 tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag atcccttcac
    16201 ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct tctaaaacag
    16261 attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc atgaatatct
    16321 ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt aatgtgccgg
    16381 ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt gaacctttta
    16441 ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg ggtacggaga
    16501 tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag atccattcag
    16561 aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg gccaatccta
    16621 caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct ggtgcttttt
    16681 tactgctggc attttctact aattctaata atttgctgat agtagaattt tcataagatt
    16741 tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat actgtttcac
    16801 caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg actaatgatt
    16861 ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca agtacaaact
    16921 gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc tgcacgtgag
    16981 ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga ctgactgcca
    17041 atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct tctggatatt
    17101 caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca tcaaagattt
    17161 ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc agcagataaa
    17221 tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc aataaagcga
    17281 tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca tttccatgtt
    17341 cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat tccttcacta
    17401 ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca atcgttagtt
    17461 ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga actccttttt
    17521 cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt ttcattttaa
    17581 tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat gaacggttat
    17641 tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa aatttcttcc
    17701 atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact gctcttatcc
    17761 aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag cctgttgctg
    17821 gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca atccctgcaa
    17881 aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga aagagttctt
    17941 taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa agacgtaaaa
    18001 gtcacatgga agaacttata caggagaacg catagagtca ggtgaaatac atgtatccca
    18061 cctgactcta tgctttatct aacctttatt attctctttt atacaatttc tgcaccgaat
    18121 ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat ccctatgatc
    18181 gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag gcctgaaacg
    18241 atcagccaga tgatattaac aatcaatgac atgccagact ggtgataaat tacttctttg
    18301 ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat tgggatacct
    18361 atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc gattagtccg
    18421 ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct tctattactg
    18481 tttttgatta aaactgggca aaaaatgatt ttattcttct ttttttagct tccagtatca
    18541 aatattctgc aaattttttt tggatttctt ccgtattttc ttttgttact aatgtgtgtt
    18601 ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc acgtccgctt
    18661 gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt ttattcttat
    18721 catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca gtaatcagct
    18781 gattgatttg aggtagatct tcttttactg cccaccgtat ttccatcatt tctcactttt
    18841 ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat ctatccagct
    18901 ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc ttgtatattt
    18961 cgaaaaacca taaacaatcc ctaaagcatt gaatatacca tctaaaggaa ccagcaatgc
    19021 gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt ctttacgcca
    19081 gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga tgttgcagat
    19141 tcccactgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt gctgagcaga
    19201 tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat tgattccgat
    19261 caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc gtacttgcag
    19321 atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca tcgttggata
    19381 ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat cagttgcttc
    19441 tgctcggtct cttcctgcgt ctgtaatgct atcaccgata aataatatac gttttttcat
    19501 ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta tcccataagc
    19561 cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta gtcccttcaa
    19621 acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt cttaacgtgc
    19681 cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac ctaagcttcc
    19741 gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19801 tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt ggtcataact
    19861 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat tgcatgctga
    19921 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19981 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat taccttcttt
    20041 gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    20101 tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa aaaaagaact
    20161 gcgagagttc gcagttcttt tttagaataa acggtgctct ccggccaact cctcggtcac
    20221 aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct tgtgactcgg
    20281 agctgaacgg caggcgcaca acctcttcct taaacggtgt tcaaaagcca gtttctcggt
    20341 attaagccaa atttatgcaa aaatgtggaa agccatttcc acaaaatttt tcttaatact
    20401 cgaaactaaa cagctttttc acaacctctc cactaaacgg tattcaaaag cttacatctg
    20461 cggtaataag tcccgccaat tcaaaaacat gaacagctgt ttttgcttgg ctgttctaat
    20521 tacttgatgc agaacagctt tttcatcacc tcttatggat aaattcggtt caacaaacgt
    20581 gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac tgttcgttca
    20641 agacctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc aagataccat
    20701 gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa atcatagtct
    20761 gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat catatctgca
    20821 cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt gatggctttt
    20881 ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt ttcatgaggt
    20941 gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag taattcgatc
    21001 gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa gacttctttg
    21061 tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg tactaagaat
    21121 gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc catttctggt
    21181 tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc acggaaagtc
    21241 ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta taattggcca
    21301 ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc cgtagtgcct
    21361 tctggtgcag aaccagtcaa gatcggcgga tcaattttga caaaatgatg ttgattaaag
    21421 aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg ttgtcttgaa
    21481 gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc ttttggtgtg
    21541 atcggataat cgtggctctc gcttaccact tccacatttg taattccgat ttcataacca
    21601 aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt ttcttggtgt
    21661 agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac tacaccttgg
    21721 aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga tcgcttattt
    21781 gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc gatgatttga
    21841 atttgttcca cgtatgactc tccttttata ttccttgaat ttctttagga ttaattttga
    21901 cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt agcgcgatca
    21961 gtagcataac tcatgcggac attttctggt gccccaaagc cttcgccagt cacaagcgct
    22021 acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata tttacacatg
    22081 cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa
    22141 acacccggca gttctgccac aagcgaatat aacgtattca ggcgttcttc aaaggcgttc
    22201 cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc atattgactg
    22261 actgctgtcg gattactggt tgactgggaa gcaacagcga tcattccatt gatgatcaca
    22321 ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt agacacgccg
    22381 ttgatgatga tagtctgatt tttgattgct tctgaaatcg ttgcaattgg agtaaactca
    22441 ttgccattat aaactaaacg accataaata tcatcagcaa ctatcaaaat atcatttttt
    22501 actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc agtcggattc
    22561 gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc tagctgttca
    22621 acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag tccttctgcc
    22681 agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac ttcatcttgc
    22741 gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc tgttacgact
    22801 gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac gattgcctgt
    22861 cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccgtt gcggattgct
    22921 tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa
    22981 ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc tgccaaagtg
    23041 acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat tcctttacct
    23101 tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc aggtaataat
    23161 tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca tcatacatcc
    23221 ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc tgttttgctt
    23281 cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca gatttaggaa
    23341 tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag tatgtcttct
    23401 ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc tttgcgattt
    23461 ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa aaaatcgagc
    23521 tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga ttaatcgttt
    23581 tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt
    23641 atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc atttttcttt
    23701 aagaattcat ggatgtcctg atagatttcc tccatagcta gctctttgat cggtactttt
    23761 ttcggtaatg catggatgat ccgtgcacca tattttgcag tgactagtct tcgatccaaa
    23821 ataagcaata cgccacgatc tgtctccgtc cttatcaagc gacctaatgc ttggcgtaac
    23881 ttcaaagctg ctttaggtac ggattcctgg taaaaaggat tgatcccctc tgattctaaa
    23941 taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa acgtgtgact
    24001 acaacgatct gcaaagcatc cccaggcaaa tctacacctt cccaaaagct gtcagcgcca
    24061 aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg gcttccgcca
    24121 aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat tttctgatag
    24181 accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc ttcttgttcg
    24241 atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct gacttgatgg
    24301 acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc ataaggagag
    24361 gcaatcactt taagaggtgc tgcttcaatg ccaagccgct tagagagata gtgtcgatta
    24421 gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg ataccaaaca
    24481 gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg ctgcgctgtg
    24541 ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca ttgatccatc
    24601 agcaagtact gcttgtctaa ttgatcaaat aagctatata aacttgctaa atcgatccgc
    24661 atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg tgactgtagc
    24721 tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga ttccagtggc
    24781 agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc gttaggttgc
    24841 ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc gcatagcgca
    24901 gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt cggtaaatag
    24961 ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg tttatggaaa
    25021 tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa atgatgtact
    25081 tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg tgtttcttgt
    25141 attaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt ttgcaagaaa
    25201 cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat acctctgtgc
    25261 gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc caaatcaccg
    25321 gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata ttgtttttgt
    25381 tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat caatatagtg gcgatagctt
    25441 ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg aatgtctttt
    25501 tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg tttttcaggt
    25561 gtagccaaat aactcaatgg cagcagataa ccgattgttt tacccattcc tgtagctgct
    25621 tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc attcatcagt
    25681 ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt
    25741 tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac ttcttttttc
    25801 tgcaaagcta gcccatcgac gatttctaat gagggatcaa gtggctctgg tttttctttc
    25861 atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg tctacttaat
    25921 cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc gatcagcaaa
    25981 agaagttgac ccgtcacttc ggcatcacta tctgcctgat gaggattctc atgaataaat
    26041 ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa gaaaatttgt
    26101 gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca tcgcatgagt
    26161 tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat ggtgtctgcc
    26221 aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg gacgcgttga
    26281 ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg atgaatatcg
    26341 atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa ttggatgatc
    26401 cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata agttcttggt
    26461 ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata ctacaaagtg
    26521 cagaaaaata ctctcatcgc tttttaaaga aaatagacaa aaacgcccag acacgcaaat
    26581 gtctgggcgt ttatttttta gtataggtta attaagcttt agtaacgttt gttgcttgag
    26641 gtccgcgttg gccttcttca acatcgaaag ttactgcttg gccttcttct aaagttttga
    26701 atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt tctgcagtga
    26761 tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc ataataaaaa
    26821 tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat ctggaagatg
    26881 tttatttgaa acaagctacc acagattacc gaacaaaaac tactcctata gtatatcata
    26941 ccgtttacaa aaaagctaga gtttttcgac tttttttatt aaaaaaagtt ttctcagcga
    27001 ttacataaag tgaaaaaccg aacttttttt aaaataaacg acatattgtt cagttggtgt
    27061 ttttttgctc tgatgttctt ctttaaacta aaaaaattat tctttctgac tacttatcgg
    27121 ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt tctcatggta
    27181 tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga tcaacgagat
    27241 tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg tccatcatat
    27301 ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg ccagcaactt
    27361 tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa agaccgttct
    27421 tctgtttcag gaatcgcaga atcaagatct gttgtctgta cataaacatt tttgtacaga
    27481 cgtgtgcctg taataggaga ccgctgtgtt tcttcagcaa aaactggctg acttgttaaa
    27541 cttagggata gaaacagcaa acaccataac agtctttttt tcataaagac ctccttatcg
    27601 ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca ttttgttcat
    27661 aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc acaacctc
//
LOCUS       DACECB010000034        27083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECB010000034 DACECB010000000
VERSION     DACECB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.034"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO04_001532"
     CDS             7..624
                     /locus_tag="IWO04_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213150.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWO04_001533"
     CDS             1644..2813
                     /locus_tag="IWO04_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0213151.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IWO04_001534"
     CDS             3286..3840
                     /locus_tag="IWO04_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0213152.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IWO04_001535"
     CDS             complement(4013..4486)
                     /locus_tag="IWO04_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0213153.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IWO04_001536"
     CDS             4498..5034
                     /locus_tag="IWO04_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0213154.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IWO04_001537"
     CDS             5074..5445
                     /locus_tag="IWO04_001537"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0213155.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IWO04_001538"
     CDS             5729..7000
                     /locus_tag="IWO04_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0213156.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IWO04_001539"
     CDS             7245..8270
                     /locus_tag="IWO04_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0213157.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IWO04_001540"
     CDS             8564..9673
                     /locus_tag="IWO04_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213158.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IWO04_001541"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IWO04_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IWO04_001542"
     CDS             10668..11492
                     /locus_tag="IWO04_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213159.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IWO04_001543"
     CDS             11524..12834
                     /locus_tag="IWO04_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0213160.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IWO04_001544"
     CDS             12982..14241
                     /locus_tag="IWO04_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0213161.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IWO04_001545"
     CDS             14251..15123
                     /locus_tag="IWO04_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213162.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IWO04_001546"
     CDS             15135..15965
                     /locus_tag="IWO04_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213163.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IWO04_001547"
     CDS             16005..18188
                     /locus_tag="IWO04_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0213164.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IWO04_001548"
     CDS             18249..19469
                     /locus_tag="IWO04_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0213165.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IWO04_001549"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IWO04_001549"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0213166.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IWO04_001550"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IWO04_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IWO04_001551"
     CDS             22431..22640
                     /locus_tag="IWO04_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0213167.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IWO04_001552"
     CDS             22633..22782
                     /locus_tag="IWO04_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0213168.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IWO04_001553"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IWO04_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWO04_001554"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWO04_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0213169.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRIKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IWO04_001555"
     CDS             25304..25537
                     /locus_tag="IWO04_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0213170.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IWO04_001556"
     CDS             25534..25941
                     /locus_tag="IWO04_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0213171.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26392..26901)
                     /locus_tag="IWO04_001557"
     CDS             complement(26392..26901)
                     /locus_tag="IWO04_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213172.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         9320 a         4024 c         5035 g         8704 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgataaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttatgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatact tggatatgct ggaatgttta agagaataat tacatttcta
    26101 gaaaatacaa taaaaattac aggataatta gccatatcct acctcctcta gcagttgctc
    26161 aaaagttagc actaagtaac ttcttttttg ttgctggtaa caatttatca atactaaaat
    26221 agcgtttaag tatctaataa atactaattt taaagctctt aattattatg aaacacgata
    26281 taacaatatt gctatatcgt gctattgtaa tattatacat ttgaagacag cgtattcttg
    26341 ttctaatatc aggaattttt tataaatatc agtcatatta agactcaaaa tttattggta
    26401 tgttcctctg tatagagttt gaccgtctac cattatatag caattattat cattgttgtc
    26461 ttgcaccaat ataccgttaa gggtagcact aggagtagta acaatcactg gaaactcatg
    26521 agaaagctcg gtcatttttc ctgttctgct agaatcgtgt tctattgtca agccatttac
    26581 tataaacgtg aatactttac ttcctattgt tatagtagct ttttgagaaa cagtttctgt
    26641 agctggagct gtgcttcttt ccgaatttgt tagggtttca ctggattcat tgctataaac
    26701 aattgtagag gattctacgg cttcttgagt gtccacgttg gtgctgctag cttcatctag
    26761 cttccttttg tattgttcgt tactgattgc taagctttcc cgttggtcat caagcttatt
    26821 catttctgta atggtataaa ataatcctcc tgctagtgct atattaacaa ctaaactaac
    26881 cgcaaataat acctttttca ttcttccttc tcttctttct ctttttgtaa ttccttactt
    26941 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
    27001 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
    27061 acaaatcgtc aatctttact tag
//
LOCUS       DACECB010000035        26540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECB010000035 DACECB010000000
VERSION     DACECB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.035"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO04_001558"
     CDS             complement(99..1229)
                     /locus_tag="IWO04_001558"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0213173.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO04_001559"
     CDS             complement(1878..3080)
                     /locus_tag="IWO04_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0213174.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO04_001560"
     CDS             complement(3140..3343)
                     /locus_tag="IWO04_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0213175.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO04_001561"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO04_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO04_001562"
     CDS             4880..5692
                     /locus_tag="IWO04_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0213176.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IWO04_001563"
     CDS             complement(6716..6868)
                     /locus_tag="IWO04_001563"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213177.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWO04_001564"
     CDS             complement(7059..7937)
                     /locus_tag="IWO04_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0213178.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IWO04_001565"
     CDS             complement(7976..9094)
                     /locus_tag="IWO04_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0213179.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IWO04_001566"
     CDS             complement(9091..11190)
                     /locus_tag="IWO04_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0213180.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IWO04_001567"
     CDS             complement(11241..12062)
                     /locus_tag="IWO04_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0213181.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IWO04_001568"
     CDS             complement(12059..12868)
                     /locus_tag="IWO04_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213182.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IWO04_001569"
     CDS             complement(12889..13383)
                     /locus_tag="IWO04_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213183.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IWO04_001570"
     CDS             complement(13395..13883)
                     /locus_tag="IWO04_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0213184.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IWO04_001571"
     CDS             complement(13960..16656)
                     /locus_tag="IWO04_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0213185.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IWO04_001572"
     CDS             complement(17084..18157)
                     /locus_tag="IWO04_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213186.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IWO04_001573"
     CDS             complement(18154..18981)
                     /locus_tag="IWO04_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213187.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IWO04_001574"
     CDS             complement(18982..19788)
                     /locus_tag="IWO04_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213188.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IWO04_001575"
     CDS             complement(19800..20885)
                     /locus_tag="IWO04_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213189.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IWO04_001576"
     CDS             complement(20902..21444)
                     /locus_tag="IWO04_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0213190.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IWO04_001577"
     CDS             21702..22691
                     /locus_tag="IWO04_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0213191.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IWO04_001578"
     CDS             22688..23425
                     /locus_tag="IWO04_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0213192.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IWO04_001579"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IWO04_001579"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0213193.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IWO04_001580"
     CDS             23989..24621
                     /locus_tag="IWO04_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0213194.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IWO04_001581"
     CDS             24706..25353
                     /locus_tag="IWO04_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0213195.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IWO04_001582"
     CDS             25358..26341
                     /locus_tag="IWO04_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0213196.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8116 a         5605 c         4500 g         8319 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa
//
LOCUS       DACECB010000036        26217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECB010000036 DACECB010000000
VERSION     DACECB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.036"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1481)
                     /locus_tag="IWO04_001583"
     CDS             complement(207..1481)
                     /locus_tag="IWO04_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ0213197.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1789..2595
                     /locus_tag="IWO04_001584"
     CDS             1789..2595
                     /locus_tag="IWO04_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213198.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            2607..3827
                     /locus_tag="IWO04_001585"
     CDS             2607..3827
                     /locus_tag="IWO04_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213199.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            3817..5403
                     /locus_tag="IWO04_001586"
     CDS             3817..5403
                     /locus_tag="IWO04_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0213200.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            5442..7580
                     /locus_tag="IWO04_001587"
     CDS             5442..7580
                     /locus_tag="IWO04_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213201.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            7868..9259
                     /locus_tag="IWO04_001588"
     CDS             7868..9259
                     /locus_tag="IWO04_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0213202.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            9318..10127
                     /locus_tag="IWO04_001589"
     CDS             9318..10127
                     /locus_tag="IWO04_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0213203.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            10099..11088
                     /locus_tag="IWO04_001590"
     CDS             10099..11088
                     /locus_tag="IWO04_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213204.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            11202..12197
                     /locus_tag="IWO04_001591"
     CDS             11202..12197
                     /locus_tag="IWO04_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213205.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            12201..13343
                     /locus_tag="IWO04_001592"
     CDS             12201..13343
                     /locus_tag="IWO04_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ0213206.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            13340..14767
                     /locus_tag="IWO04_001593"
     CDS             13340..14767
                     /locus_tag="IWO04_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0213207.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(14834..16123)
                     /locus_tag="IWO04_001594"
                     /pseudo
     CDS             complement(14834..16123)
                     /locus_tag="IWO04_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(16303..16713)
                     /gene="tagD"
                     /locus_tag="IWO04_001595"
     CDS             complement(16303..16713)
                     /gene="tagD"
                     /locus_tag="IWO04_001595"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ0213208.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            16839..18230
                     /locus_tag="IWO04_001596"
     CDS             16839..18230
                     /locus_tag="IWO04_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ0213209.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            18363..19310
                     /locus_tag="IWO04_001597"
     CDS             18363..19310
                     /locus_tag="IWO04_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0213210.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(19350..20510)
                     /locus_tag="IWO04_001598"
     CDS             complement(19350..20510)
                     /locus_tag="IWO04_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0213211.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            20675..21850
                     /gene="mltG"
                     /locus_tag="IWO04_001599"
     CDS             20675..21850
                     /gene="mltG"
                     /locus_tag="IWO04_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0213212.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            21986..22465
                     /gene="greA"
                     /locus_tag="IWO04_001600"
     CDS             21986..22465
                     /gene="greA"
                     /locus_tag="IWO04_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0213213.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            22633..23364
                     /locus_tag="IWO04_001601"
     CDS             22633..23364
                     /locus_tag="IWO04_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0213214.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            23361..24428
                     /locus_tag="IWO04_001602"
     CDS             23361..24428
                     /locus_tag="IWO04_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0213215.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            24435..25067
                     /locus_tag="IWO04_001603"
     CDS             24435..25067
                     /locus_tag="IWO04_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213216.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            25207..25872
                     /locus_tag="IWO04_001604"
     CDS             25207..25872
                     /locus_tag="IWO04_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0213217.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            25900..26193
                     /locus_tag="IWO04_001605"
     CDS             25900..26193
                     /locus_tag="IWO04_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0213218.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8861 a         3960 c         5105 g         8291 t
ORIGIN      
        1 acctaaaatg aaaatggggc tgaagaatca attctgattc atcagtccca taaattatag
       61 agcctaaatt atagagccca aaatacgaga gaggctgtga caaccgtcgc agcctttttt
      121 ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat aaaacttagt ccgatatgcg
      181 gcgcgttctg tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag
      241 atccgtgccc gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg
      301 atcttgttat tcagcccctc agtgacgccg tttgagtggg acaccttaaa ggcatttgaa
      361 atacccgatt ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta
      421 gatgaaattg ttttaagggt ttcttgaaag ccttccgtac ctcgtttaac gaaataatag
      481 cgtaataatt gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca
      541 tcaacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt
      601 ggctcccatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac
      661 ttcttctgat tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga
      721 ttgaagctgc gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt
      781 ggaaagactg ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg
      841 acctttaaac gggcttttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg
      901 cgatcttcaa ggatcgtaaa tagctgatgg gttgtcccat caacacaaat aaaactcatg
      961 gaaccagaaa cagacttcat tgatttgaac tcatccatac acaacaccgt cggaaggaag
     1021 ttcatacgtg gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc
     1081 gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga
     1141 atcgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca
     1201 gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt
     1261 aacttttctt tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata
     1321 cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac
     1381 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
     1441 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
     1501 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag tttaaacgaa
     1561 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaacc
     1621 ccttttttgg tttattgcaa ttgtctagca tcttctgtaa aatagaaacg ttagagaaaa
     1681 tcttgtatca tagaaaatta agaaagaaat aagaacgcag ctcggtacca aaaatatggt
     1741 aaaatggcac ttgactgtaa ttcactgaaa tcgcttggag gaacacaaat gtttaaagat
     1801 ttgtatttat ttatgaaaga catttatcag aatagaaaat tactgctaca gttttcgatc
     1861 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
     1921 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
     1981 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
     2041 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
     2101 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
     2161 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
     2221 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
     2281 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
     2341 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
     2401 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
     2461 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
     2521 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
     2581 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
     2641 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
     2701 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
     2761 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
     2821 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
     2881 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
     2941 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
     3001 ttgaatttgc tgatattggc gaccatattt atcaacctgt aaaaacctat tcaagtggga
     3061 tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg
     3121 atgaagcatt ggcagtagga gatcttgaat tccaactgaa atgtatggaa aaatttacag
     3181 aaattaagaa ctcgggaaaa acgattttat tcgtttctca tgatattagt tcgattcgcc
     3241 gtttttgtga tcgttcctat tggttgaaaa atggagaagt tgtcgaatat ggtgatacga
     3301 tggaagtaac gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc
     3361 gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg
     3421 aattgttgga taaaaataaa aatccagtgg atatgctgga acaaggagaa aaactatacg
     3481 ttaaagtaac ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc
     3541 tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga
     3601 tcccttggaa aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag
     3661 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
     3721 acaagacaaa atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
     3781 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
     3841 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
     3901 ttagagtttg gtcccggaaa cggtcgatta acgaaacatt tgatcggagc aaaacaatgt
     3961 gaagtgagca ttgttgagct agataaggaa ttattcgatt ttgtcagcga atttgcccaa
     4021 gatggatttt atggagatat cgaaagtttt gaatgggcaa actattatgc tggacagaca
     4081 tttgattacg tcttgtttgc ggatgtttta gaacatttag tagatcctgg aaaaacattg
     4141 aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaactta
     4201 gcacataatt ctgtcatgat cgatttgttc aataatcaat tgccttgggc ttcttatggt
     4261 ctcctagatg aaacccataa ttctttttat actcatgatg gtttccaaaa agtttttgaa
     4321 aaagccggat tattcattaa tattgaagat tacttgtact tggctgtagg agatacagaa
     4381 ttaaaatcaa cttatgaaga gttgccggaa gctgtacgct atgatttcaa aatgcgacca
     4441 tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagt acaaacttcc
     4501 attgcacagc cacaaaactc taattatgta aaagtactag aagtaaccca gcaaacgaaa
     4561 caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa tgagacacta
     4621 agtttccccg tgtcagaagc aacagaaaga atggtgtttc gcttcgcaca gcaacctagc
     4681 tttatcgaat tcagtgcaga agctgctgga gaaaaactca tatttatcga ttcaaatgct
     4741 gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaaatacc agagttcgct
     4801 ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa
     4861 ttgacaccaa caatgaaaga attgctggaa acaattcgtc ctatggctga agtcacaaag
     4921 cagctttctg agaaaaatga tgaattagaa agagaaaatc agcatcttct tgaggaaaat
     4981 acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt
     5041 gatgaatgga cgatcaaaca tagactagga cgacgtaaga aaaaaacatc gaaaaaaata
     5101 caagaaaaag agctttctat atgtattgat gagaaaatat gggatgcaga aacaaaaata
     5161 ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agccattgtc ctataagtta
     5221 tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagatga agtcaatcaa
     5281 gcagaacagc tcgcaaaagg aacagaagcg ggatttgaat tacgaatcct ttgcgagcaa
     5341 gaacgttcat tcttagtgga agcagtagct caaaatggtc aatcatggat cattgaaatg
     5401 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
     5461 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
     5521 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
     5581 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
     5641 agacccaaag cggttttaag atacgtttat ccgggaaaat gagaggaaaa gcgatcttag
     5701 attttcaaac ggcaaaacat caaaatggca tagctgtgaa actaaatggt aaataccctt
     5761 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
     5821 attatgcaag gactcatggc gtaaaaaaaa caatccgccg ggtcaaactg gaattgaaac
     5881 caggatcgat cgactatgca gagtggatca gtcgtcacga gaaacctgat ctgaaagagc
     5941 aaaggaaaga agcacaaggg tttgcatacc gtccattgat ttccatactt atgcctgtct
     6001 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtattggat caaacgtatg
     6061 atcactggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
     6121 tggaatccta tcaagcaaag gatgatagaa tcaaagttgt tttccgacaa gaaaatggac
     6181 atatcagtct ggcaactaat tctgcgttgg aaatggcaga aggagaattt atagcgctct
     6241 tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaaca
     6301 tacatccaga actggatttg atctacagtg acgaagataa aatcgatgca gatggcaatc
     6361 gctttgatcc gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata
     6421 tctctcactt aggcgtatat cgaacaagta ttgtgaaaaa gcttggcggt ttccgtaaag
     6481 gctacgaagg ctctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag
     6541 atcatattta ccatattgac cgagtcttgt atcattggcg gacgatccca ggatcaaccg
     6601 caagtaatgg tgaagccaaa agttatatct atgattctgg agtgaaagca ttgacagatg
     6661 ccctgtctag aaggaatatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg
     6721 agattgctta tgacgtcttg caagaggatt tggtcagcgt gatcattcca acgaagaatg
     6781 gctatgaaga tctaaaaact tgtgtggatt cgattatcga aaaaacgtct tatccaaatt
     6841 atgaaatcat tattgcagat aatggaagta cagatcctaa aatgcaggaa ttatttgcag
     6901 aatacaagca tcagctgaaa gaccgattta tcgttgaatt aattgacatt ccattcaatt
     6961 attcacgtat caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga
     7021 ataacgatac agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt
     7081 ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg
     7141 caggagtatt attaggaatc ggtggtgttg caggacatgc attgaataac tatgatcgaa
     7201 cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
     7261 cttgtatgat ggtcaaggca gctgacttca atgctgtcaa tggatttgat gaaacattag
     7321 aagtagcatt caatgatgtt gacttgtgtt tgaaagttta tgaactgggt cgttacaatg
     7381 tctatgcaca tcaagcagaa ttgtatcatt tcgaatctaa atcaagaggt tatgaagata
     7441 caccagaaaa acaaagacgt tttgccgggg aaataaaaaa aatgcaagat aagtggcctt
     7501 cttatattgc tcatgaccca ttttataacg ataatctaac taaagaagga atcggcgatt
     7561 tttcattgag acctgattga ttaaaagaac tgtattttca tttttattga tgaaaataca
     7621 gtttttttag tatatgaaaa ttgtaacttg tgaccgaaga gatgatagga agaacaccca
     7681 ttcagtagga agaatgtgat acgatttttg tccaactctc tgttaggcag aaatatacat
     7741 ataaacatac aatagtcacc ttttctagtt tcttttaatt tattttacaa tttggttatt
     7801 cactaggttt ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag
     7861 tgtatatatg aataaaaatg gggagtggaa tgcggcacgt aggatattca ttatcatcat
     7921 ggatgtactt gtattgaatg tgtcgatcta tcttagcttt ttgctgaagt tccaaggaga
     7981 agtcccagca agaaacttag agacctttca gcattcagca gtttttattt ccattatctt
     8041 tattgcgttg aatattttgt taggagctta tgtattctat agccgagtga tcagtgatat
     8101 cgtcttcatt actgttattg gacaggtgct gatagcattg gggatcatgg tagttacttt
     8161 tgctggacgt tggtttgcat ttccgcgtgg cgtcatcgtg ctctcgttta tacttggcac
     8221 gatattactt agcgtttatc ggatagttgt ttataaacta tacttaagag tcagtcgtga
     8281 taagaaagtc cttatcgtag gtttggagaa agatgtcgca ccagctatcc aaaactttca
     8341 atctaagaaa aacaacaaac ataaagttga agcagtagtg atcgacaagt attacgaaaa
     8401 cacaaaaaaa ttgattgatg atatcgatat tgtctacttg gcttctaaaa tcccagaaga
     8461 cgaaaaaatc aatatttatc aattagtaac taaaaaagag aagaagctat ttttaaatac
     8521 aacttttgaa aatttaacga tgatcaatcc gaacatcatg aatttcgaag atgaaagtat
     8581 cattgaagct tccggtttca aaattcagcc ggaatatgaa ttattcaaac gtctttttga
     8641 tatcatcgtt tcgcttattt tactgattct tgcttcacct tttatgctaa tcacagcgat
     8701 tttggtaaaa acaacttcac ctggaccaat tatttataaa caagtacgta ttacgaaaaa
     8761 tcaaaaggaa ttttcaatct ataaatttcg ttccatgagt gtaacagctg aagcaaaatc
     8821 aggaccggtc ttggcaaaga gcaatgatgc acgtgtgaca ccagtaggca aatttatccg
     8881 tgccgtacgt tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat
     8941 cgtcggacca cgtccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta
     9001 ttattattta agacacaatg tacgtgcagg gattaccgga tatgcacaag tatacggaaa
     9061 atatgtatca gattataata gtaaactacg ttttgatttg ttgtatatca aaaagtattc
     9121 cttactatta gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc
     9181 ctcacaagga ctagatgaag atgaaaatat ccgttcgtta gcagaaatcg acttttcaca
     9241 agagaaaatt tatcgctaga tgggaaaaat tttgagcaaa gaagtaggag agaacattgg
     9301 aaaataaaaa tataagtatg ctgctttctt tatatgataa agaaaaacca gagtatttaa
     9361 aggaagcatt aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt
     9421 atgacggccc tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc
     9481 ctcacttgtt tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa
     9541 ttggattaga acatacgaaa aatcgcttag tagccagaat ggatacagat gatatcatgg
     9601 aaccccaacg attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg
     9661 ttgggagtaa tattgaggaa tttactggca cgcttagcaa tgttatcgga agaaggattg
     9721 tacctgaata taatgatgaa atccgtgtgt tttcgaaaag aagaaatccc tttaatcata
     9781 tgacagtcat gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg
     9841 gatttgaaga ttattatttg tgggtaagac tgttgaaggc gggatataaa gggtataata
     9901 tccaagagat tctagtccat gctagagcag gtgctgacat gtatgctaga cgtggaggaa
     9961 gtaaatatct tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat
    10021 cactaaaaga ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata
    10081 aaattagggg aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc
    10141 ataccttttt acaatgcaaa aaatacttta aaaagagctg tagacagcgt cttaacgcaa
    10201 ggttatccca atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac
    10261 attggtgacg aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct
    10321 ggtccatcag ttgctaggaa ttgtgggata aaagaagcaa ctggaaattt ggtaggattt
    10381 ttagatagtg acgattattt tttacctgat tgttttaata atgttttgaa agactaccat
    10441 aggaattacc ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca
    10501 aatttatttg ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg
    10561 tttagaagta aggcagtgga tttttacgta tggaataaat tttttaaaag ggaattattc
    10621 aatcatatag tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct
    10681 tttagagcgg ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat
    10741 aatgatagca tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcaaaga
    10801 aaaatattag ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct
    10861 cgattagtag acggatacct ttcaacaggg tttaaaatta ctggcaagaa tcaaaatcag
    10921 aaagaaaagc aaaaatatta taatcagtct aaaagagaaa taaaaagtat atattcaaaa
    10981 ataagaagta acaatgagat atcaattgct aaaaaattag ctttattttt atacttaatt
    11041 aatcctaaaa cttattttat tttatataaa aaagttctaa aaaaataaag tttttttcga
    11101 tatgttattt tttatgttta ttaacttcgg aagactaaaa ttgttactgg taatgataca
    11161 tttgtaaaaa taaaaaagat gatttttagg aagtgaagag agtgcataaa attagtgtta
    11221 tagtacccgc atataacgta gaagactatt tagaaaactg cttagaatct atttccaacc
    11281 agacacattc aaatattgaa ataatagtag tcaatgatgg ttccccggat cgaagtgggg
    11341 aaattgcaga agaatataaa aagaaagata tgcgattaaa agtgatacat caagtgaatg
    11401 gtggtttaag tgcggctagg aattctggtc ttgattctgc aacaggagat tttgtgtgct
    11461 ttattgacag cgatgattgg atagaaccag actatcttga gttactatat tttggcacgc
    11521 aaacttatgg agcagatatc tcggtgctaa aaataaaaaa gatttcaagt ttagaacaac
    11581 taaaagaaca tacaattgct gagagaaaat gggaaatatt tgatagagaa caagcaatga
    11641 aaaacttatt tataaataat aaaattggct attctgcaaa taataaatta ttcaatagaa
    11701 atttatttga taatataaga ttcccactgg gtaaattaat ggaagataag gctactactt
    11761 atttgctgat cgatcattcc gaaaaagttg ctgtcaattc atctgctaaa tatcactatt
    11821 accaaagccc taaaagtata ctgagaggaa gcttcaatcc tagaattttt gattcttttg
    11881 aagtccatga agatatacta atattcatag ataagtttta tccggaatta tcaattaatg
    11941 ttagaactcg ctatgtctat gaagcaataa ggatgatgat gacaatgatt aaaaataact
    12001 ataccaataa agaagattat ttacgttcta taaatatttt acaacgcttt gaaaaagaag
    12061 tgaaaattga tcccgacttt tcaccaaaat taaaaaaatt agtaaagttt ttattaataa
    12121 ataaaaactt gccttactat atgtcaagta gtaagatagc aagtaaattc ttgattaaga
    12181 tagaatctgt gaggtaagag atgaagaata taaaaaaatt tttttcaagg aatattttcc
    12241 cggtttttat taaaatatta cctattcaaa aaaatttaat tgtattttca agttttggcg
    12301 ggcgtaacta ttctggaaat ccaagagtta tttcagagaa aattcatgaa aaaaaattga
    12361 attacagaat tatttggtta atcaaaaaag aagcgattat aactgattta ccagattata
    12421 ttaaaagaat tgactccgat tcttttttag ctgcctttta tttatataca gctaagtatt
    12481 ggatagacga tagtcggaaa acgattaagt atgcaaaaag gaaaaaacaa ctttattttc
    12541 aaacatggca cggaacgcct ttaaaaaaaa ttgaatttga tgcaaaagat aagttaccta
    12601 aacgttacct aagctatgcg aaaaaagata gtgagtcaat tacatattta ttatctggta
    12661 atcgctatag ttctaagaag tatgtttcag cattcaatat cgttccttct aaaatattag
    12721 aagtaggaac tcctagaaac gatgaattag cctctagtaa agagcaagtg tttgacttga
    12781 aaaagaaaca aatcaatgtc ttatttgctc caacttttcg aaataatatt gaggacaacg
    12841 gaattacgca actagaatgg ttaggtgtgg ataacttacg tgaatttttt aaaaagcaac
    12901 aacaagaatt aaattttatg actaaatttc atcctaatgt tcatagtaaa ttagcaactg
    12961 attctaaaag tatagaatat atgaaaaaac atcaaattac tttaattaat gatgggattc
    13021 ctcttgaaat gctttttgaa gagatagatg ttttaatcac agattattca tctatttttt
    13081 tcgattttgc gctaacaaaa aaaccaatta ttctttttaa ctatgatgaa aaagaatata
    13141 gcttagaaag aggtttttat atacagttaa gtgagttacc aattataagc gtacaagata
    13201 gttatggcct tgttgatgtt tttaaaaacc aacaagaaaa gcttttgaaa agttctcaag
    13261 agttacttga ttatattgga aattatgaaa caggagcaag tacagaaaag gttcttgagt
    13321 tgatagagag ggatataaag tgaaaaaaac agtaaaagat atattttata atgcgatata
    13381 tcaagtcttt ttgattgttt tacccttact tactatacct atacttagtc gtagaattgg
    13441 ttcaactggt ttagggattt atgggtatgt tttctcaata tcacagtttt taatgactgt
    13501 cattgctgtg ggaatgaatc cttttcgtat tcgaaatatc gctaaatcaa ggaaagataa
    13561 aaaagcacta tctttacaat tctggaatat ttactttatt caatttttaa taggcttgag
    13621 tgtttccttt ctctatatag cctatattta tttttttgat gtaaagtata aaaatttata
    13681 cttaattcaa ttgatattta ttgtagggct aacattagat atttcttggt tttttcaagg
    13741 aattgaagaa ttttcgaaag tggttattag aaatacagtt atcaaattat catcggtatt
    13801 gttaataatc atttttgttt caactaaaga tgatttgtgg atttacgtgt tcattacttc
    13861 aattactaac tttttaggaa gtttagtttt ctggttaagt atccacggaa aagtaggtaa
    13921 accaatcttt aactctaatg tttttaaaca gttgtggaaa ccagctttta ttattttgac
    13981 tcctcagttg tttatgcaag tgtacacaac tttagacaaa acgatagttg ggtattttgt
    14041 ggatccaacg gaattatctt attatgatca gtctcaaaaa attgctcgta ttatattggc
    14101 agtcttaaca tcaattacta ttgttatgtt gcctaaaata tctaaagcac aggcagaggg
    14161 gaaatcagaa aatattttag tttacacaaa aaaatctttt gattacacga ttatcattgc
    14221 attaattctt tttagtgtgg tgtttatcaa tacaaaagaa tttgttccat ggttttttgg
    14281 ggagaatttc aaaccgatga caataaacat gcttatttct tgtctaatta tagttgttag
    14341 tccaattggt ggagtttttg ctaaccaatt tgcaattgct atagaaaaag ataaggagta
    14401 tgcttatcca atgatcattg gtgcgattgt aagtattatt ggaaatgtag cttttgtgcc
    14461 tacttatggc gctgtagcgg ctacgatcgt actcgtggta gtagaattat tagtcttttt
    14521 ctttagagta ttttttgtac gaaaagaaat aaaaatatcc tatttctttg ggaagaacgt
    14581 tatcttattt tttatagtca atcttacttt gactacttgg gcctattact ttcttccaca
    14641 attattctct tcttcatttt tagatatggc aattaaatca ataatgattt tcattattta
    14701 tcttccgttt gtttattttc tttttccaga tatacgaaat gaaataagaa agattactcg
    14761 aaaataaaat aatgttgtaa aaaagtagtt ctttgtcaaa tcgggctgat aaggtcattt
    14821 atgaacaaca aagttagtaa gagttactag cttttaatgc atagctgatt aacctgttga
    14881 ttagaaaaat ccacaagcgc atattggtaa aactgcgaaa cccataagca aatcgtttca
    14941 atgttttgat atgtgtgcga agattctcta attttgttat tagaatactt tttcgaaaat
    15001 gctagttaaa agcctttttc agatatgaaa aaggcttttt gagatagtga agtttcgagg
    15061 gaacttcttt ggtcgtttct cgaagtagct agctgtttct tgtgataatt gctaagcagc
    15121 ttttgataaa ctgcttagca attttaagtt cataagagat tgagggtaga cgatcaacta
    15181 cttcactttc tgttaaatag cgccattgaa aattctgcca tttcttgaat gttgtgtaat
    15241 ccaactcgtc atctgatttt agaagtaatt tatacaaagg tttcaattgt cgatagtact
    15301 tagcttgtga agtgtctttt tgattcaact gcttcatcac acgttttcga acacgatcga
    15361 aaaacttgtt caagtgttgt ccaatgtgaa agcaatcaat gatgatttgg gcgtttggaa
    15421 aggactcttt gactaatgaa aaataagggg cattcatatt tgtgacaatg tatcgaaccc
    15481 ccatatgagc ttttcttgta aaacgcatga aataagcaat caaatctttt tttctccggc
    15541 tggacaaaat gtcaaagagc ttccctgtct ctctattcat acaagaaaag gagaactttc
    15601 catgggaaat tgaaagagag cgaaactcgt cgaatagtaa acaatgaggt aaatagtcta
    15661 gcatctgtcg cagcattgcc gatagagcag ataatgtccg ttgaaatgtc gttactgaaa
    15721 gatcacaacg tttcgtaagt gtctttaacg cttgacgctc ggtcaactct tctaatatcg
    15781 tgtaatgaac ttgtttcgaa ataaaacatg aaggaacgac aagatacgta tgagcagtaa
    15841 aatattttcc gcatgattgg tagtggtaat gttgttttct aagacgcaaa taggtttatt
    15901 tattcccact gcgatttaag ataatcattg aaatcttctt gccgtttttc acgagctgct
    15961 tcgcagtttc tttacaaaat agacaatgtc taaaagtgcg aactaatgta ccttcgatta
    16021 cgatggcttt cttgtcctta taaaaagttt tttgaaaaga attaatttca atatttttat
    16081 ctttaatagt gaagtgctct ttgatagaac gagaaatgga tatacaaaat cttcctttca
    16141 gattgatgtt ttagacgact ctattttatc gaaatttttg tatgtctgtt ttttttacaa
    16201 gaaaatctag gatcgacaga aaattctatc agtcctagaa attatagagc cattttttta
    16261 cgtcttatcc agatatattg ttctttcatt gtttaaaaaa tattatttat cactatttaa
    16321 agaattaagt tcgttcttta tttgagttgt agagatctca ggtgttcttg gtaaataaat
    16381 tacttgtgca tctgttagtt gttcaagttc atcaaattgt ccagcccaat catcgcccat
    16441 cacgaaataa tctgcatggt attcttttat atcttttact ttttgttccc aattttcttc
    16501 aggaataact aaatcaacgt agcgaatagc ttcaagtaac tgttttcttt tttcataact
    16561 aaaataacat tttttttgtt tcgcattcca attgaattca tctgtagaaa gggcaacaat
    16621 aagataatct ccatattgtt ttgctcgttt cagtaaattg atatgcccgt agtgaagcaa
    16681 atcaaaggta ccgtaagtaa ttactctttt catctttcac ctctcgaaat tcattaatat
    16741 acattgttag tttagcattt ttttgttaga attgaaagaa ccagtttatc ctaaaaagga
    16801 taagaaatgt ttatgagaaa taggtggatg gaggaaaaat gttgaatgaa aaatttttaa
    16861 aatatgtgaa aggttggtat tttatttatt ttggttttat tctaatgtta cgagcaatag
    16921 gcccgtattt actattacct actagatatg atactttact tttttggcct gctggaatag
    16981 cgggagttgt cttgatcatt atagatgcaa ttattgcatt tagagaaaaa aatcttaaaa
    17041 aatatgattg gctattaatt gcttttattg gcgcgatgct agtaagcagt ttaatgaatt
    17101 taaaatatgg atatagcgaa aatatcaagt taatcatgtg gcaattaatc tttttcttta
    17161 tgatttatca gtttggcaaa gactacagta gtaacttttt tttcaaagta tttacttgga
    17221 tactttcatt ggtatggttt ttagctaatt tagtatcatt atactggttt gtcattcaat
    17281 atggcataaa aattccaata aaatataagt attacccagt tcgtttaggg tggttaggca
    17341 accgtttatt tggtgtatat acagatccaa attttggtgc ggttatatca ttaattgtca
    17401 tcattattgc tatatattat ttggtcactt acaagcattt aaatcttcta tttaaaatat
    17461 tttttgcaat taatattttt attcaattct cgtttattgc tttatcgggc tcacgcacca
    17521 cattagtttg tttattaata gcaacttttg tttttagttt ctttacaagt tatagtagtc
    17581 gttttcttaa aactaatact ttaaaaagat ggctaactgc attagtagta ggattgctaa
    17641 gtataattgc gatgtattat gtaacagata taactgctca agcatgggca catgttccgc
    17701 atattacaga aataaaaaaa gatatatctg attcatcagg aacaactagc agcaataaag
    17761 ttgatttaaa acgccctgac actgatggcg gggatgtttc taatttaagg tttggacttt
    17821 ggaaaagtgc aattgaaata ttcaagagta agccgatatc cggcggttct ccaggatact
    17881 atattgaata cgcacatgat aaactaccat atacattaat gggaaaagat actttaactg
    17941 ctcataattt tattttctta gttatggctg ctacaggagg attaggtcta ttgacattgt
    18001 taaccttctt atttatccaa tgctttaaaa ctttgcgtta ttgtttcaca caaccaaatc
    18061 tagtaaaaga taagcacttt tttgctattt taatgattct tacaatagca gtttcggcat
    18121 tatttatcac agaactcatt ttggttagta cgataggttc atttattttt tggggatatc
    18181 tgggttctat ccaaaaaact atatctaaag aagaaggagt aactaattga aactattttt
    18241 aggtatataa agtagaaaag aaaactagtt atcccaactt caacaaattt tatagttttt
    18301 acaatataaa aatggtaaac taactttatt aagtatgaga atgcataatt ggagtgaatt
    18361 tgatggatac tatattgatt actggtggag ctggttttat cggttcaacg ctagcgaatt
    18421 atttgggaaa agaaaacaaa atcgtagtag tcgacgattt atcaatggga aaaaaagaaa
    18481 acttggatat ggagaaacaa gtgactttca ttgaggggga tgtatcagat cctcaattga
    18541 tggaaagaat catgaaacag tatcagtttg cctatatttt tcatttagct gctgtagcta
    18601 gtgtggcaga ttctgtagaa cgtcctctag aaactcatcg tgtaaatttt gacagtgcgc
    18661 ttttattgct tgaattggta cgaaaatatc aatctggttt aaaacgattg gtatttagtt
    18721 catcagctgc agtttatggc gatgaaccaa cgttgccaaa aaaagaagaa tcggtcatcc
    18781 gtcctttaac accatatgcc atcgataagt ttgcatcaga acaatatgtt ttggattact
    18841 gtcatttata tgatgttccg actagtgctg tccgattttt taacgtttat ggtccaaatc
    18901 aaaatcctaa ctctccatat agcggagtta tttcgattct agtggaccga tacaaaaaac
    18961 aattggcagg agaaaaaaca gaatttactt tatttggtga tggtagccaa tctagagact
    19021 tcgtttatat agaagatgtt atacaagcac ttttacttgt tgctaaggaa gaaaaagctt
    19081 taggacagca attcaatgtc ggtacaggaa aaagcactac gctacttgaa ttgattcatt
    19141 caattgatca aatcttagga acagagctag cgcttaaata tgaagcggag cgttcagggg
    19201 atattcgtga ttctttagcg gatatttcaa agatccgttc tttaggttat caacccaaat
    19261 ttgatatatt gaacggaatg gaacgttatt taaagacaga aatcgattga aacaaaaaac
    19321 cagcatcctg ctggtttttt tgtctcgact tagcttttta aattaacacc ttccatcagt
    19381 tcgtaaatct gatttcttaa atcagtggcc ggcaatttga tgtttttatc agattggata
    19441 ttactttcca tgacgattgc atcttttgtg tcttttttca tcgccgctac tgcatggaat
    19501 cgattgatat ttccctgagc aaggatataa tcatttccac tgtaaacacc gccgctgtaa
    19561 ggatgtttat caccattatt ttgccacaat gagaaaagta gttcttttgg tataatatcg
    19621 ccagacagaa cactggtaaa gaatttgttc aagtcataaa cagacatact tatatttcct
    19681 gtgcctaatt catttcggat gtctgtttca ggctcgacca atgcttttga ataatcgtca
    19741 gttgcagaca tttggtaaga tacagcatgc tgaggggaat tttttacatt ttcataaaaa
    19801 tctgtatgtt ctagatggag tggccgaatg atttcttttt ttaaaagagt ttcataaggc
    19861 tgattcatta gtttgatcaa tacccctgta agcaatgtat aatttaatgg ttcataatga
    19921 tacgtatcat aaccagtaaa tttcaaatga tgaagggcaa actgaatgat ctcactatct
    19981 gacataggtt tttttggaga agcggttcga tgaagaccag agcgcatgta aagcatattt
    20041 ttgattgaga tttgctgact tccaggaatc tgaggataaa attcagatag ctttgtgtca
    20101 aaagttatct tgccagattg aatttgtttt aggataagta aagcagtcat cgctttttgg
    20161 atcgaaccga tttgatagta aagagaagga gaattcgatt cttttatgcg tagatttgcg
    20221 tacccatatc ctttttctaa tatcactcga ttatttttgc gtacataaat cgaaccgata
    20281 taatctgatt tttgcagtaa ttgatcaatt ttgctggcaa gcatctgctc attagatgtt
    20341 aaattggaag caagttgtgc gggttccgtt ttataaattt gtggttgagg ttgttttgtt
    20401 aattgccatc ctgtaatgat gcctgttgaa aagaagctga cagccagcaa caaaactgcg
    20461 agaaaagaaa gagagatctg ctgttttttt tgatgttttc cttttgccat attaaatcct
    20521 ttctatccat ttttaactat ttttcattgt aatcctcaaa aagagcattt gccacttaca
    20581 attttgacat aatatttggt aaaataattc acggcagtag aaatttcatg tataataaac
    20641 agagttacga agatgatagt aaggaggagt tgttttgtca ggaaacaaac aacacccaag
    20701 caaagacaaa aaagaagcca gtaaggcagc tacagcagat ataatcgaca agcggagaaa
    20761 tctgcgtaaa aaagaagata agatcgtgag gaagatcatt ttagtgatcg ctcttacatt
    20821 attgattatt ggcggatttc tcgggtttac cgtatatcgt tatgtagata gcgggttgaa
    20881 gcctttagat aaatcggacg accaattagt ccaagtggaa atcccaaacg gctcttctaa
    20941 taagcagatt ggcgaaattt tggagaaaga caatattatt aaaagtggga ttgttttcaa
    21001 ttattataca aaatttaaaa acttaactgg ctttcaagct ggttattatc agttagcgcc
    21061 taatatgact cttgatgaga ttggcaagca gttgcaagaa ggcggcacat ctgagccaac
    21121 aaaagttgca gatgggaaaa ttgccattcc agaaggctac gacatcgatc agattgctga
    21181 gcgggtagcg aaggtaacag gtaaagacaa gaaagaattt ttagatttag taaatgatga
    21241 gactttcttt aacgagttgt atcaaaaatt cccagaattg ttggaaagtg cctctaaagc
    21301 tgagaacaca aaataccgtt tggaaggcta cttattccca gctacttatg attactacac
    21361 gagtacttcg cttaaggatc ttgtgattga gatggtgaac aagacaaata cggtcatgca
    21421 aaactattat tcagctatca aacagaaaaa tttgactgta caagaagttt taactttggc
    21481 ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt aaaaatattg cacaagtctt
    21541 ctttaaccga atcaaggcaa atatgccact tcagtcagat atctctgttt tatatgcatt
    21601 aggagaacat aaagaattgg tgacgtatga agatacagct gtagattcac catacaattt
    21661 gtataccaat accggctatg ggcctggtcc ttttgacaat ccaagcgaaa aggcaatcaa
    21721 ggcagtatta gaaccagcag aaaatgacta ttattatttc gtagcagata cgagcactgg
    21781 aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa ctagtgcaaa aatatgtaaa
    21841 taattcttag aaaaacaggg gctagtcatt gatcattttt agaaaaacat ttacatttta
    21901 agaaaagcgt ggtaatcttt tttggactat attcctcaaa agattacctt ttgcggtatt
    21961 ttatcgaaaa aaggagacgt tacacatggt agagaaagtt tatccaatga ctctagaagg
    22021 taaagaaaaa ttagaaaaag aattagaaga actaaaaaca gttaaacgcg gagaaatcat
    22081 tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc gaaaactccg aatacgaatc
    22141 agctaaagat gaacaagcat ttgttgaagg aagaatcaca acaattgaaa atatgatccg
    22201 ttttgcacaa atcatcgata atgacggcgt agatgcagat gaggtatcga tcggtaaaac
    22261 agttacgttc atcgagttgc ctgatggaga tgaagaagag tacacgattg tcggaagtgc
    22321 tgaagcagac ccgttttctg gaaagatttc caatgattct cctatcgcgc aagcattgat
    22381 tggcaaacgt aaaaatgatg aagtaaccat tgctacacca ggtggcgata tgttagttaa
    22441 aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc tagagagttc atcttactga
    22501 tgacgcactc tagatctttt ctctttgagt attgcaggtt tcactgaacg atcttacatc
    22561 taaagtccta tagccaacag tcaaaaaaat aggtatgatg aaatgagaat gtgtcaggag
    22621 ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg tagtcgaagc actgctgctg
    22681 ttatttgctg tttggcaaat cgtaaacaat actggacttt tgcttttagt aatattcggt
    22741 attttcaata tatatctagc tatgcgcaaa tatcctcgta ctaaatttca aaattttcaa
    22801 ttgatcttag ggagcttggt catttttttt agcttagtca acagtcctgc tttatggatg
    22861 atggttgtat tggccgtttt atttatcggg ctgaaaggtg tagagatttc aggaatagat
    22921 ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga tggtccaaac agagcaatta
    22981 aaaacacata ataatgagcg taaaagacag caattatttg gtaaccaacg tattggaaac
    23041 gacgtctatg aatgggatga tatcaacatt gccatcattt ctggtgatac tatcatcgat
    23101 ttgggtaata cacttttgcc aaaagatgat aatattgtga ttgtaagaaa agggataggc
    23161 agaacacgga tcttagtacc cctgggagtg gctattcgtt tagagcatgc gacattagta
    23221 ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa acgaacagat caaaatctgc
    23281 agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca ttaccaatac cttggttgga
    23341 gatgtcgagg tgatacgaat atgatgggaa gaatatccaa agcaatgcta gctgtttatt
    23401 cggggattgc ggctttcctt attattctat tttcactttt cacttatttt tatgccagta
    23461 accaaagtca ttggtgggga gaattgctgc gtgcgcgttt attatacgtt ccgcttatct
    23521 tccatttgat ggcaatttca ttaggtgtag gattgatcgt atttctattg ttatcactta
    23581 ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg tgcactttct tctggcaatt
    23641 atgaatccaa acttttgcat cagccgatcc caagcgcgtc ggatgatctg tacatcaaag
    23701 atattgatat ggaaattgct aaaataaaag aaaaaatgat cgaagtatcc agtgaattac
    23761 agattatgac tagccgtcct caatatgtag atggacaaac caaagaagag attttagaat
    23821 tagaaaggca tcgattagct cgtgagcttc atgattcagt ttcacagcaa ttatttgcag
    23881 cgatgatgat gatgtcggca ttgacggagc aagcagaaaa aagtgagaca ccagaaatgt
    23941 tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc ttcccagtct gagatgcgtg
    24001 cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa aagtttaaaa caaggcattg
    24061 agcagttatt gaaggaattg cagaataaaa tccagatttc gctaaaatgg gatgtagaag
    24121 atgtaaaatt aactagttcc attgaggatc acttgttccg aatcgttcaa gaattgttat
    24181 caaacacatt aagacatgct aaggctaatg aattagaagt ctatttgcat aaaatagata
    24241 acaatctttt attacgtatc attgacgacg ggacaggatt taatatgaat gaaaccaaaa
    24301 cgggaagtta tggattgaac aatatcaaag aacgagtagc tggtattggt ggaacagtaa
    24361 aaatcattag ttttaaaggg caaggtacga gcgtggagat caaggtccct ttgatgaagg
    24421 aggcatagat aaagatgata aaagttttat tagtagatga ccatgaaatg gtacgcttag
    24481 gcgtctcttc ttatttatct atccaagaag acattgaagt gattggagaa gctgaaaatg
    24541 gacgtcaagg ctatgaaaag gcgatggcac ttcgaccaga tgtcatttta atggatctag
    24601 tcatggaaga aatggatggc atcgaatcaa caaaagcaat cttaaaagat tgtccaaaag
    24661 ccaaaatcat tatcgtcaca agttttattg atgatgaaaa agtttatcct gcaattgaag
    24721 caggagcagc aggatatttg ttgaagacgt ctaccgcaca tgaaatagct gatgctataa
    24781 gggctacgca acgcggcgag cgagtattgg aacctgaagt gacaacaaaa atgatggaaa
    24841 aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt aacgaatcga gaaaatgaaa
    24901 ttttgatgct gatttctgaa gggaaaagta atcaggaaat cgcggatgaa ttgtttatca
    24961 cactgaaaac agttaaaacg catgtgtcga atatattagc taagttagag gtagaagacc
    25021 gaactcaagc agctatctat gcgtttaaac atggtttggt gaaataaaaa cgatgataaa
    25081 gaggataatc aaaagctgag aaaataacta ttatttttct tggctttttt acaataatga
    25141 tgcttgcttt tgtttttgga aaggctttgt tatactgaga aaagaagata ctaaaggagg
    25201 aaaatggtga aacaaaattt tgcaattgta ggccttggac gatttggcgg aagcatctgc
    25261 cgtacactga tcgaagctgg acaagaagta ctggctattg acagtaatga agatcgagtc
    25321 aatgaatata tgaatatagc cactcatgct gttgtggcaa atgcacagga tgagatgaca
    25381 cttcgttcat taggtataag aaatttcgat catgtaattg tcgctattgg agaagatatc
    25441 caagccagta tcttagttac tctgatggta aaggaaatgg gcgtaccgaa tattttagca
    25501 aaagcccaaa atgagtacca tgcccgtgta ctagagaaga tcggagcaga tcgtgtcgta
    25561 caccctgagc gagacatggg gatacggatt gcacataact tagtatcaaa gaatattcta
    25621 gactacttag agctgtcaga taaatattct ttagcagaaa tccgagtctc caatcctaaa
    25681 tttttcaaca aaacattagc tgaattaaat ttcaggcaac gttttgactt aacagtagtc
    25741 gccatacgaa gagtagatca aacggttatc gcttctcctg cagcagatga atatgttcgc
    25801 gaaaatgata atctactggt gattggagag acaggcgatg tggatattct agacgataag
    25861 atgaatcaat agaaagaaaa gagaaagtag gttgttggga tggaattaac ggtaacgcca
    25921 aaagcacaaa agtggtttga ctcagaaatt gatctgccag aaacttacgg aatacgtttt
    25981 tttggaaaag tctacggcaa aacagaggtt catgatggtt tttcgattgg aatgtcagtt
    26041 gaacagccag aacatcctgt aaagaaagtg gaaatggatg ggacactttt ttttatcgag
    26101 gaagcagatg aatggttctt taaaggatat gatttacttg ttgattatga tcccaacctg
    26161 gaggagccaa cctatcactt taaaaaacaa taaaaaaaga gagggtgaca caagttt
//
LOCUS       DACECB010000037        25998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECB010000037 DACECB010000000
VERSION     DACECB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.037"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            289..606
                     /locus_tag="IWO04_001606"
     CDS             289..606
                     /locus_tag="IWO04_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213219.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="IWO04_001607"
     CDS             complement(697..1062)
                     /locus_tag="IWO04_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213220.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="IWO04_001608"
     CDS             1270..1752
                     /locus_tag="IWO04_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213221.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /gene="pstS"
                     /locus_tag="IWO04_001609"
     CDS             1949..2839
                     /gene="pstS"
                     /locus_tag="IWO04_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0213222.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="IWO04_001610"
     CDS             3165..5639
                     /locus_tag="IWO04_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0213223.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="IWO04_001611"
     CDS             complement(5742..7478)
                     /locus_tag="IWO04_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0213224.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="IWO04_001612"
     CDS             complement(7475..8179)
                     /locus_tag="IWO04_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213225.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="IWO04_001613"
     CDS             8310..8813
                     /locus_tag="IWO04_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0213226.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="IWO04_001614"
     CDS             8773..9111
                     /locus_tag="IWO04_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0213227.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="IWO04_001615"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="IWO04_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0213228.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="IWO04_001616"
     CDS             10662..11240
                     /locus_tag="IWO04_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0213229.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="IWO04_001617"
     CDS             11244..11765
                     /locus_tag="IWO04_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0213230.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="IWO04_001618"
     CDS             complement(11844..12398)
                     /locus_tag="IWO04_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0213231.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="IWO04_001619"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="IWO04_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0213232.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="IWO04_001620"
     CDS             12938..13189
                     /locus_tag="IWO04_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0213233.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="IWO04_001621"
     CDS             13313..14104
                     /locus_tag="IWO04_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213234.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="IWO04_001622"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="IWO04_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0213235.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="IWO04_001623"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="IWO04_001623"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0213236.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="IWO04_001624"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="IWO04_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0213237.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="IWO04_001625"
     CDS             16339..16581
                     /locus_tag="IWO04_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0213238.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="IWO04_001626"
     CDS             16932..17186
                     /locus_tag="IWO04_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0213239.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="IWO04_001627"
     CDS             17215..18474
                     /locus_tag="IWO04_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213240.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="IWO04_001628"
     CDS             18527..19423
                     /locus_tag="IWO04_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213241.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="IWO04_001629"
     CDS             19434..20303
                     /locus_tag="IWO04_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213242.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="IWO04_001630"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="IWO04_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="IWO04_001631"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="IWO04_001631"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0213243.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="IWO04_001632"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="IWO04_001632"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0213244.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="IWO04_001633"
     CDS             23711..24208
                     /locus_tag="IWO04_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0213245.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="IWO04_001634"
     CDS             24393..24653
                     /locus_tag="IWO04_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213246.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="IWO04_001635"
     CDS             complement(25015..25254)
                     /locus_tag="IWO04_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213247.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="IWO04_001636"
     CDS             25540..25854
                     /locus_tag="IWO04_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213248.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8635 a         4373 c         5306 g         7684 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agtttata
//
LOCUS       DACECB010000038        25363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECB010000038 DACECB010000000
VERSION     DACECB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.038"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(137..514)
                     /locus_tag="IWO04_001637"
     CDS             complement(137..514)
                     /locus_tag="IWO04_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213249.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /locus_tag="IWO04_001638"
     CDS             770..1498
                     /locus_tag="IWO04_001638"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0213250.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="IWO04_001639"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="IWO04_001639"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0213251.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="IWO04_001640"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="IWO04_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0213252.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="IWO04_001641"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="IWO04_001641"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0213253.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /locus_tag="IWO04_001642"
     CDS             4633..6072
                     /locus_tag="IWO04_001642"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0213254.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6104..7147
                     /locus_tag="IWO04_001643"
     CDS             6104..7147
                     /locus_tag="IWO04_001643"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0213255.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="IWO04_001644"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="IWO04_001644"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0213256.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="IWO04_001645"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="IWO04_001645"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0213257.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="IWO04_001646"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="IWO04_001646"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0213258.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="IWO04_001647"
     CDS             10666..11580
                     /locus_tag="IWO04_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0213259.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="IWO04_001648"
     CDS             complement(11713..13278)
                     /locus_tag="IWO04_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0213260.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="IWO04_001649"
     CDS             complement(13271..14722)
                     /locus_tag="IWO04_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0213261.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="IWO04_001650"
     CDS             complement(14736..15752)
                     /locus_tag="IWO04_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213262.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /locus_tag="IWO04_001651"
     CDS             15972..17060
                     /locus_tag="IWO04_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213263.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /locus_tag="IWO04_001652"
     CDS             17114..18640
                     /locus_tag="IWO04_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213264.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /locus_tag="IWO04_001653"
     CDS             18642..19847
                     /locus_tag="IWO04_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213265.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="IWO04_001654"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="IWO04_001654"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0213266.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21345..22769
                     /locus_tag="IWO04_001655"
     CDS             21345..22769
                     /locus_tag="IWO04_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0213267.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23230..24339
                     /gene="pdhA"
                     /locus_tag="IWO04_001656"
     CDS             23230..24339
                     /gene="pdhA"
                     /locus_tag="IWO04_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0213268.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24342..25319
                     /locus_tag="IWO04_001657"
     CDS             24342..25319
                     /locus_tag="IWO04_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0213269.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8205 a         4317 c         5697 g         7144 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagggtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    21301 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    21361 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    21421 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    21481 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    21541 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    21601 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    21661 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    21721 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    21781 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    21841 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    21901 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    21961 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    22021 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    22081 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    22141 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    22201 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    22261 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    22321 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    22381 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    22441 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    22501 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    22561 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    22621 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    22681 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    22741 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    22801 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    22861 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    22921 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    22981 tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca
    23041 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    23101 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    23161 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    23221 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    23281 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    23341 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    23401 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    23461 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    23521 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    23581 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    23641 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    23701 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    23761 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    23821 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    23881 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    23941 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    24001 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    24061 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    24121 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    24181 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    24241 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    24301 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    24361 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    24421 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    24481 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    24541 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    24601 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    24661 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    24721 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    24781 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    24841 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    24901 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    24961 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    25021 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    25081 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    25141 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    25201 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    25261 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    25321 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACECB010000039        25066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECB010000039 DACECB010000000
VERSION     DACECB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.039"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            92..871
                     /locus_tag="IWO04_001658"
     CDS             92..871
                     /locus_tag="IWO04_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213270.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            912..1856
                     /locus_tag="IWO04_001659"
     CDS             912..1856
                     /locus_tag="IWO04_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0213271.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1872..2651
                     /locus_tag="IWO04_001660"
     CDS             1872..2651
                     /locus_tag="IWO04_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0213272.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2664..3362
                     /locus_tag="IWO04_001661"
     CDS             2664..3362
                     /locus_tag="IWO04_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0213273.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3390..4154
                     /locus_tag="IWO04_001662"
     CDS             3390..4154
                     /locus_tag="IWO04_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0213274.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4199..4888
                     /locus_tag="IWO04_001663"
     CDS             4199..4888
                     /locus_tag="IWO04_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0213275.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            5231..5404
                     /locus_tag="IWO04_001664"
     CDS             5231..5404
                     /locus_tag="IWO04_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ0213276.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            6751..8001
                     /locus_tag="IWO04_001665"
     CDS             6751..8001
                     /locus_tag="IWO04_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0213277.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            8020..9030
                     /locus_tag="IWO04_001666"
     CDS             8020..9030
                     /locus_tag="IWO04_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0213278.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            9040..9693
                     /locus_tag="IWO04_001667"
     CDS             9040..9693
                     /locus_tag="IWO04_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0213279.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            9712..10710
                     /locus_tag="IWO04_001668"
     CDS             9712..10710
                     /locus_tag="IWO04_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0213280.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            10786..11547
                     /locus_tag="IWO04_001669"
     CDS             10786..11547
                     /locus_tag="IWO04_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0213281.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            11565..12671
                     /locus_tag="IWO04_001670"
     CDS             11565..12671
                     /locus_tag="IWO04_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0213282.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            12661..12894
                     /locus_tag="IWO04_001671"
     CDS             12661..12894
                     /locus_tag="IWO04_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213283.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            13093..13548
                     /locus_tag="IWO04_001672"
     CDS             13093..13548
                     /locus_tag="IWO04_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ0213284.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            13562..14335
                     /locus_tag="IWO04_001673"
     CDS             13562..14335
                     /locus_tag="IWO04_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213285.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            14322..15452
                     /locus_tag="IWO04_001674"
     CDS             14322..15452
                     /locus_tag="IWO04_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0213286.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            15483..16718
                     /locus_tag="IWO04_001675"
     CDS             15483..16718
                     /locus_tag="IWO04_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213287.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            16743..17852
                     /locus_tag="IWO04_001676"
     CDS             16743..17852
                     /locus_tag="IWO04_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213288.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            17894..19228
                     /gene="gmhB"
                     /locus_tag="IWO04_001677"
     CDS             17894..19228
                     /gene="gmhB"
                     /locus_tag="IWO04_001677"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ0213289.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            19263..20900
                     /locus_tag="IWO04_001678"
     CDS             19263..20900
                     /locus_tag="IWO04_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0213290.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            21002..21397
                     /locus_tag="IWO04_001679"
     CDS             21002..21397
                     /locus_tag="IWO04_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0213291.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            21415..22146
                     /locus_tag="IWO04_001680"
     CDS             21415..22146
                     /locus_tag="IWO04_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213292.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            22170..23621
                     /locus_tag="IWO04_001681"
     CDS             22170..23621
                     /locus_tag="IWO04_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0213293.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            23647..24258
                     /locus_tag="IWO04_001682"
     CDS             23647..24258
                     /locus_tag="IWO04_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0213294.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            24440..24955
                     /locus_tag="IWO04_001683"
     CDS             24440..24955
                     /locus_tag="IWO04_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ0213295.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT         8280 a         3682 c         5405 g         7699 t
ORIGIN      
        1 tttaattatg cttttaaaat aaaaagcgct cattctgatg ggaatgaatc acatctttat
       61 ttatctatca tcaatattta ggagtgaatc tatggaaaag ccgttcgtca agttattggc
      121 tactgtggcg gtaggcacag gcgctattgt gatttgcttg tttggatacc atttcaataa
      181 tcagcggcaa catcatcaac ggatcaatta cgcagaatct gccatcacca atcaaaaaga
      241 caccgtgacc tctttatcaa aagaagtaga taagctgtac agtacaaaag aaaaaatttt
      301 cctaaatcct gaaataacag aagaaacagt ttcaaatttg tcacataaac tatcttctat
      361 caaactttcg gcagatgatt atgacatcaa ggaatcagaa ttacctaaag aagctgctgc
      421 gatccaagaa gaaaagaaag cagtcttaac gcagttagag gatgcagaat caaaactgaa
      481 gatccagaca gctgtcaacg atttatttac taaaaatgta tcaaactggc agcaagctgt
      541 agacgatgtc gtcatcaaag aaaaggtgac aagtgcagat gtcgctcgtg tacgtgaaaa
      601 tatgagcttt tttaaagata gtccttggaa aacggttgtt atgcaatatc taggattcgc
      661 tgatactcag atcgcgcaag tcacgcaact agatcagcta ttcgatacga tgctgaaaga
      721 cggtcaagta acagctactg ctacttacga tcaatatttg accgcactca gtctcatcga
      781 acagatccgg aatgaaaaaa taagagccgc atacgcgtca aaagcagaaa cagtcgctca
      841 gcaaatgggc tatagcaata cgagctatta ataagcgaga attcaagcaa ggctagcaaa
      901 cggaggaaac tatggcaaaa cgaagaagaa gatcaagagg gaaaaagata tttctaatta
      961 ttttagcaat tcttgtcgta ttagtattag caatcgtagg agtaggagca aaattatatc
     1021 tagatctctc acattctatt gaaaaaactt acgaatcagt ggaacgaacc caaggagaac
     1081 gaacaaaacc agttgatctg gacaaacaag aatctttctc tgttctattg atggggattg
     1141 atacgggaga tttaggtcgg gtcgatcaag gaagatcaga tacgatgatg gttgccacag
     1201 tcagtcctga agacaaacag acaacgattg tcagcattcc tcgtgatacg tatgtcgaca
     1261 tcgttggaca tgacacaaaa gacaagatca accacgctta cgcattcggc ggagctgcca
     1321 tggcgatgga taccgtacaa aaatatgtag acatcccaat cgatcactat gtttcaatca
     1381 atatgaaagg tctaaaagaa ctcgttgatg cagttggtgg tatcgaagtc aataatgacc
     1441 tcaccttctc acaagatggc tatgatttta cgatcggtaa aatcacattg aacggggatc
     1501 aggcattgtc ttattctcgt atgcgttatg aagatccaaa tggtgactac ggcagacaag
     1561 aacgtcaaag aaagatcgta gaaggtgtag cgaaaaaagt gttgagtctg aacggtgtaa
     1621 gcaactatca atccgttttg aatgctctag agtcgaatat gaaaacagac atgagctttg
     1681 atgaaatgaa aaagattgct ttagattatc gggatgcatt tggtacgatc aagcaagatc
     1741 agctgcaagg agaaggcttc atgcaagaca atatctcata ccaaaaagtc agcgatcaag
     1801 aacttgatcg tgtgaaaaaa gaattaaaag atcaaatgaa tctagagaat aaataggaaa
     1861 gtggagaaaa aatggaagag acaatcagct tgaaagaaat ttttgaaaca ttacgcaaac
     1921 acgtgataac aatcctcatc tcaatgtttg ctggcttggc catctcaggc atcgtgacgt
     1981 ttttcatctt gacgccaaaa tacagctcac aagcccaatt gatcgtgaca ctgccgcaat
     2041 cagatactgc aaatgtcaat gatgtcaata cgaacctgca aatgatcaat acgtataagg
     2101 atatgatcgt cagtgatcta gtactaaatg aagtcaaaga tcgtttagaa acagaagaca
     2161 atgtaaaaat gactgctgga caaatcaaag atgccatctc cgtcaatcag tcacaaaatt
     2221 cacaaatgtt ctcgatccaa gcaacaagca caaatgcagt gacggctcaa caaatcgcta
     2281 atacaacgtc acaagtattc caagagaacg ccaaagacgt gatgaatgtc gataaaatct
     2341 cgatcatctc aggtgcagtt gccaatacga cacctgtttc gccaaataat aaattgaatc
     2401 tagtgattgg cttagtgtta ggaatgatgg taggcgtagg tctagctttc ttatttgaat
     2461 tactcgaccg gacagtaaaa gatgaaaaat atgtgacgga tacattgggc ttcccgatct
     2521 tagggactgt accagaaatg tcagcgaaag aattgaatgc tggtatccgt aaaaaaggac
     2581 cagctccgac acaaccaaaa gctacaaacg ataaaacagc gccatcacgc agaagccgct
     2641 caagagtcta ggaggaaaga aaaatggcac gaacacagaa acaaaaagca gcccaaactc
     2701 atgcagtcag tttggtcaca ctcacaaacc cctcttcacc aatcgctgaa caatatcgta
     2761 cgattcggac gaatatccag tttgcctccg cagctggtca gcaaattaaa acgattgttg
     2821 tgacttcatc aggacctgga gaagggaaat cgaccactgc agcaaacatt gccgttgttt
     2881 ttgccaaatc tggtcaacgg gtgctgctag tcgatgcaga cttacgtaag cccgtcatct
     2941 ataaaacgtt caagttgaac aacgcaagcg gattaagtac cgttcttagt tcatcaggaa
     3001 gtgtagcaga tgagatccaa cgaacaccag tcgagaattt gtcgattctg ccaagtggac
     3061 ctaaaccacc aaatccatca gaactactaa gttcgccaag aatggatcag attttagcag
     3121 aagcaagaca actatttgat gtagtgatct tcgacatgcc gccagtagtt gccgtaacag
     3181 atgcacagat catgtcttcc aaaacggatg gcaccttatt ggtcgtacgt gaaaatatgt
     3241 cacggaaaga atcattgact aaagcaaaag aattgctgga tatggtacaa gcgcgagtcc
     3301 ttggtgttgt atacaatggg gcagaacaca gcaaagatgc tggctattac tacgggaatt
     3361 aagaaataaa aagagaggag acaagagaaa tgatcgatct acattgccat atcctgccag
     3421 gaatcgatga cggagcagaa aacatagatg ccagtatcgc catggctgaa aaagcgattc
     3481 aacaagggat cacacatatt ttatgcacac cccatcataa taatggaaaa tacagcaatg
     3541 aaaaatcaca agtcatttct ctagtcgctt cgttacaagc agaattagag aaaagacaat
     3601 tgcctttgac gttattggaa ggacaagaag tacgtattac cggaacactg atcgaagaca
     3661 tcgaccgaga tgaaatttta tttacagatt tagatgatac atatcttcta atcgaatttc
     3721 cgacactgga agtgccgtta tatgcagaac agctcttttt tgagttgtta caactaggaa
     3781 agacaccagt gatcgtccat ccagaacgca acgcgcattt tcgtaaagac ccgaatcata
     3841 tgctgccatt cctagatatg ggatgcttgg cccaactcac cgcaccaagt tatgtcggtg
     3901 tatttggaaa agacatccag aaaacagcca aaatgatggt caaacataat cttgtgcaga
     3961 tggctgcttc tgatgcccat ggggtctcta agcgtacctt ttacttaaaa gagtgctatg
     4021 agcagattgc taaggacttt ggtaaggaaa aagtagtaca gatgaaacaa gtcgcaaagg
     4081 atctgatcaa cggggatgaa atcagttatc cagtctatga agaggtaaaa aagaagaagt
     4141 ttggtttatt ttagttcttt attcgtagat aaaaaggaaa tgaggagaga gggaaagtat
     4201 ggaaagtgaa gatttattat ctcgtattag tgaagtacat cctaatcaag tttggttgga
     4261 taaagaaaaa gttgaaaaac gatatatcta tttatttttt aaaagaatga ttgatattgt
     4321 aggaagcatt attggtttga ttttagcttc tcctttgatg ttctatgtag cttatcgaat
     4381 cagaaaagaa gagccaggaa gtccgatatt tttttgccat acacgtgttg gtaaaaatgg
     4441 aaaaacattc aaaatgtata aattccgttc gatgtgttta gatgctgaag aaaaattgga
     4501 agaattactt gatcaaaatg aaatagaagg tgcaatgttc aagatgaaag aagatccccg
     4561 tgtcactaaa ataggtaaaa ttatccgtgc caaaagcatt gatgaattgc cacagctatg
     4621 gaatgtttta aaaggcgata tgagtctaat tggtccacgg ccacctttgg agagggaagt
     4681 agctgagtac acgagatatg atcttcaacg cttgtatgta aaacctggtt gttcaggttt
     4741 gtggcaagtt agtgatagaa ataatgttag tttccataaa atggttgagt tggatttaga
     4801 gtatattagt catcgaagtt taatgtacga cttgaggttg attttaaaaa cgttttatgt
     4861 tattgtcaag cccaatggag catattagtt agggatagaa tgatggattg ttaataaaaa
     4921 agattacaca tgttcatatt atcgtttcaa aagtaataac tgtaattatg gagggtttga
     4981 atcacttgta gaaaatctaa aaaaagaaag gtgaatgggg caattcagta tcattattct
     5041 tatataaaag aaaatattcg aaattttggg atgataccac tcttaaatat aatgatctaa
     5101 atgctttaat attggtatat ccgatatagg aactgacgag ctatttagta tgatacaaaa
     5161 gtatattgat agccattaat aaattttcca aaaaataatg acagcacacc aatttttata
     5221 atttagcttg ttgaatcggt atgttcatgg gacggttcaa aaaaagaatc gaaaaataaa
     5281 cggtaaactt ttagtgaatc ctaacggata tgagtggtta cgagcaaaag ggagttatcc
     5341 ggtcccaaaa tattgtaatt attctgaaac attaatggaa tggtatcaac tgataataac
     5401 ataataagag ataaaattga ggaatatcta tcattttata acaaagagaa tgctgcagag
     5461 tgtttactta aaatgtggga gtaaggtatt tgtgacttta gaagaggcta ttttttatct
     5521 ataagaattt attatccact gtgcagaaat gaagattata gttcatctaa acttttaaac
     5581 tttgtaatat attggatgtt cataacggaa aataatattt ggaaaagtac gttgttataa
     5641 agtatgtacc actatcttaa atattatgaa actaagacaa ttttttttga aactatctat
     5701 agatatattt actggaattt ttggaggtat agaaacagtt atcattttat ctttgtacca
     5761 ccaatgattt atatcagctc aacagagtga attgtattta cgcaagaatg cgtaggatga
     5821 aatggataga taattaagat atatatgtat atggtgactg aagacaaaaa aaacagaact
     5881 aatgaaactg aataaaaata tagagtgaac tagtttctaa gagggatttt aaactagatt
     5941 tcattggtaa taaaatttgg acagatggaa aaacatcaca taaggaagca gattttgttg
     6001 tcatttttgt tcctacaaac tatggtagtg aatgtgttat tgaattagtg atgtctgtta
     6061 cttcagaggc attggggata attaaatcca ctatacctgt aggatataca aaaagtggaa
     6121 gaaaaaaata ttatcaatag attttattta gttcagaatt tttacgtgaa agtagattat
     6181 taatgataat ctttatccaa ttagaattat tatcgtagga tgtgatgatg aaagccgtga
     6241 aattgttcaa gtatttacca atgtctccct gccagtaaat ccgcttagaa tttgagcctt
     6301 gaaaatgtgc aatgataaaa aacaaaaact cgctcaaaat agacagtttg gggtgggaat
     6361 agaccaataa tgtaccaaac aaacttccat ttggggtgaa aataggtcaa taaagcgatg
     6421 ttaaacatac ctttggagta agaatagagg gtaataagtg aatttataaa ctttttggtg
     6481 actggttgag tggaaaatgt aggtacattt caatgcgaaa atgtattata accccaaacg
     6541 aggtcaataa aaatgactca aacttgcaat ataaccctaa acgggtaggg gatttatgaa
     6601 aaattgaatt tgccggcgta ggagctggct gttttaagta tgaatgaaca cagctttcgg
     6661 gctgttttat ataaatagca ggttactgtt cacaaacagc attttgttaa gaaagtagtg
     6721 accacagcag tggtggaaag aggaaatatt atgaagaaac agtataacat tgcagtcgcc
     6781 ggcaccggtt atgttggttt gagtattgcc actctgttgg cacagcacca taaggttaca
     6841 gcggtggata tcattccaga gaaggttgaa ctgattaata accgtaaatc tccaatccag
     6901 gatgattata ttgaaaagta cttggctgag aaagaactga atctgaccgc aacattggat
     6961 gcaaaggcag cataccagtc tgcggatttt gttgttattg cggctcctac taactacaat
     7021 agccgcactc aacattttga cacatccgct gttgaggctg ttattaggct ggtcatggaa
     7081 gtaaatcctg atgccattat ggttattaag tccaccattc ctgttggata caccgcttct
     7141 gttcgtgtaa agactggtag caagaatatt attttcagcc ctgagttcct gcgtgaaagt
     7201 aaggcactgt atgacaatct gtatccttcg cgtattattg tgggtaccga tatggatgat
     7261 gagcgtttgg ttgaggatgc acacactttt gctacactgc tacaggaagg cgcaatcaaa
     7321 gagaatgtgg acacattgtt catgggcttt actgaagcag aagctgttaa gctgtttgcc
     7381 aatacatatc tggctcttcg tgtcagctac ttcaatgagc tggacactta tgcagagatg
     7441 aaaggcttga acactcagca gatcattaat ggagtttgcc ttgatcctcg tattggtact
     7501 cattacaaca acccttcctt tggttacggt ggctattgcc tgccgaagga taccaagcag
     7561 cttctggcca actacgcaga tgttcctgaa aatctgatcg aggcaatcgt tgaatctaac
     7621 cgtactcgta aggactttat tgcggatcgt gtacttcaga tggctggtta ttacaattac
     7681 gcagaagcaa atcagtatga tgctggcaaa gaaaagcctg ttgtgattgg tgtttaccgt
     7741 ttgaccatga aatccaattc tgataacttc cgccagagtt ctatccaggg cgttatgaag
     7801 cgtgtaaaag ctaaaggtgc aactgttgtg atttatgagc cgaccttgaa agatggagag
     7861 actttctttg gcagtaaggt tgtgaatgat ttggaacagt ttaaggcaat gtgtgattct
     7921 gttatagcaa atcgctacga tgcttgcctt gatgacatta aggaaaaagt atatacccgt
     7981 gatatcttcc agagagacta agattaggag agagtattta tgattattac aaaaacaccg
     8041 tttcgtatgt ctttttttgg cggtggaacg gatatggagg attatttcaa agagaatggc
     8101 ggtgcagttc tttctactac ttttgataag tactgttatg tgaatgtgag acatctgcct
     8161 agattttttg attacagcgc tgagttatct tattctcgta ctgagagagt aactagcatt
     8221 gaagatattg agcatcctgc gattcgtaat gcgatgaaaa tgctcgatat gcatgaaatt
     8281 cggcttacat acgaagctga tttacctgct cgctctggtc tgggtacatc cagttctttt
     8341 gcagttggta tgttgaatgc tttttatgca ctggagggta agtatgctga caagaagaaa
     8401 cttgctgatg aagccattta tcttgaaaga gttctgtgta atgaagccgg tggatggcag
     8461 gatcagattg ccgcatctta tggtgggttc aatcgaatta atttcaatgt ggatggttat
     8521 gaggtgctgc ctgtcattat ttcgcctgaa cgtaaaaagc agcttaacaa caatctgatg
     8581 atgttcttta ccggattcac tagattttct tctgatgttc aaaaggctaa caacgtcagt
     8641 ggcacagagg ataagagagt aagactgaaa aagatgtatg agcttgtaga tgaagctgag
     8701 gcaattctta ctgataagaa cagaaattta gatgatttcg gtagacagct tgatgttact
     8761 tggagattga aaaaaggtac tggtggagca attagtacag gaagcattga tgaactatat
     8821 gaaaagggta tggctgcagg tgctttaggc ggaaagttgc tcggtgcagg tggtggaggt
     8881 ttcttggttt tctatgtaca acctgagaaa caggatgcag ttcgttgggc tatgcgtgat
     8941 ttgatgtata ttccgtttga atttgaaaat ggtggaacta gagttattca ctacacgccg
     9001 gaaacttata ttgaaaaaca gaatggataa ggagtaacaa tgttagcgga aaaacttaat
     9061 aaacatattg acttattgat taaacgatat cctacacttg agacgattaa acaggacatt
     9121 atcgatggat acctaattat ggaggaatgc tatttgaatg gtggtaaact tcttgtggca
     9181 ggaaacggag gatcagccgc tgactcggaa catattgctg gcgagttgat gaagcggttt
     9241 aagacgccac gtcctgtaag taaggaattt gccgataagt tagttgcaat tgaccctgta
     9301 agaggtccgg aattagccaa aaatctcgag tgtggtttaa cggcaattcc tttggtcgca
     9361 cacgaagcat tgtctactgc ttatattaat gatgtcgatg gtttgggtgt cttcgcacag
     9421 cagctgtttg gctttggtag atctggtgac gtattccttg gtatttcgac gtccggaaat
     9481 agcaagaata ttatgagcgc aactgttgtt gctagggctc tcggtataaa agttattggc
     9541 ttgacgggcg caaagggtgg ggaacttgcc acggtagcag atgtttgtgt taaggctcct
     9601 gaagttgaaa catacatgat tcaagagtat cacttgccga tatatcattg ttggtgtttg
     9661 atgctggaag ataaattctt tggaaacgaa taaaatttgg aggaatgtta aatgaaaaag
     9721 acaattatgg ttaccggtgg ttgcggtatg attggctcca atcttgttaa gagactggtg
     9781 aaggaaggct gctgggatgt ttatgtagct gataatctgt ggagaggaaa gttggagtac
     9841 ctgaacgacg aagatgggca tcctgtcatc gacctggata ctcatttctt caatgctgat
     9901 ctgactgatt atgagcaggc taaaaaggtt attggtacga ctgagtatgt agttcatctg
     9961 gctgatgttg tggctggcat tgactatgtg tttaagaacc agggcgaatt gttccgaatc
    10021 aataatctga tcaattctaa tgtttttgat tgttgccgta aggctggcaa ggagaaaatc
    10081 aagggtgtta tctatgttgg taccgtatgc tccttccctc tgaccagaca gaatactttg
    10141 aatcctgagc ctctccgtga agaggagttg ttccctgctc ttcctgagtc tgcttatggc
    10201 tggagcaaac tgatgggcca gttggagatg gggtatttgg aaaaagaaac cggaattccg
    10261 tgctgtatcc tgatgctcca taatgtttat ggcacaccta ctgattttgg cgaacgtagt
    10321 caggttattc ctgctctgat tcgtaaagca atcaataatc ctgaagagcc ttttaatgtg
    10381 tggggtagcg gagagcaggg acgtgctttc attcatgtca atgatatcgt aaatgcattg
    10441 gtgctcgccc tggataaggg ttggggtcat ggtcatattc agattggccc ttctcactgt
    10501 acttccatca aggaaattgc atataagatc attgaaattt ctggcaaaga catcaagcct
    10561 ttctttgata cttctaagcc tgaaggtgat aaggctcgtt gcgcagatta ttctaaggcg
    10621 aaagagattt tggattggga gcctactgtt tctttggaag agggtcttcg tgaatcctac
    10681 aattggattg aaaagcagat taagggttaa taagtatata agctgttctt cctccgtgta
    10741 attagaggat gcggaggaag aacatatgag taaggatgta ctgagatgga aaagaatatg
    10801 tataaaagaa aacgtgattt cactgtgtgt ggaatagtag gtgtttgtgc tactacaaag
    10861 aacgagtttg taagggaagt tttttccatg ccaacatctc cggtaagtgc gactgttgat
    10921 ctcgtgggag tgccggccat tatttcggca aaggaaaatc ccaaaattgc tgaaatgtat
    10981 agccaggcta caatggctgc aatagatggt atgcctattg ttaaaattgg acggcgcaaa
    11041 ggatttacct gtgaacgatg tgctgcacca gatattatgg gaatggtttt tgaagaaggc
    11101 gttagacagg gaaaaacaca ttatttctat ggtggaaaag ataacgaagt gttaaataag
    11161 cttcgtgcaa acctagaaag agattacccg ggaatcaaaa ttgttggaat gtattcccca
    11221 ccatttcgtc cgttaaatga agaagaggat agcaagattt gcgaggaaat caacaactta
    11281 aatccagatt ttctgtgggt tggtattggt gcgcctaagc aagaaatgtg gatgtgggaa
    11341 caccagaaga agatccataa cacagtaatg ttcggtgtag gcgccggctt taactttttt
    11401 gcgggcactt tggaaaaagc tcctgaatgg atggaaaaag ctagtctaga gtggttattt
    11461 agactggtaa aggaacctcg tagactatgg aggcggtata tcttgggagg tataaagtat
    11521 atctactata ctattgaatc taagtagggg ggcaataaaa atagatgaaa atacttatag
    11581 tagttacaat gacgaataac tttggtaaga aggggtccta taattctcag gaaataggat
    11641 tagctcgagg gttagctgaa ttaggccacg aagttattgt atataaaagt gtaaaaagtg
    11701 atgaggaagt tggaacagag caattaagca atacggcgat gattcactat atcaagtgtc
    11761 gttatgtagg atcaaatgga ttcttatcaa caaagaattt tgataagaat gcggatgttc
    11821 taatacaatt tgcggacctt caactatcag tacctcatgt atactcatgg actgtaaaaa
    11881 atggaataaa atatatacct tataatggga taacgcgaag tcaaagtggc aataaatata
    11941 taaagacaat tattgaaata ttggcgcatc gaaattataa aatttataag aaaaatgggt
    12001 gtgttgccaa aaatgatcga gtttctgaaa aattagtaca attaggagta aataaagtga
    12061 aacttgctcc tgtttgtgtg gattttgaac agttacataa gaattacgaa gcaatagatt
    12121 ttactactct cagagaaaaa tggaatattc ctcgcaatgt tcgtgttatt ttatatgtag
    12181 gacggtttga aaaggataaa agaccgttag atatgattag tttatttaat aaacttgatg
    12241 agcataatta tttgctgatg gtaggaactg gtgctttact cgagcaaacg aagcagcaga
    12301 taaatttact tggactgcag aatagagtaa aaataattga gcgtattcct aacaaagata
    12361 tttgggagtt atattgctta tcggatgtat ttgtaaatct aaactatact gaaatttggg
    12421 gtatggtttt gctagaggca atgtactatc gcctcgggat tattgctgtt aatgcacctg
    12481 gcccacagaa tattattttg gataatagat atggaatgtt ggctaaatct gacgatgaaa
    12541 ttttgacatt cttaaaagac ggacgattta ccaacgatat catagatgca gcatatcagc
    12601 gtgtaactac agaatttaga tggtcaaagt cgggagaaat tatgcttagc atgttggaag
    12661 gtgataaatg atatggaagt aacgaaatta atggcattga ggaatcgata tgctttaaac
    12721 atagttgata attgcacgag aaaaatagcc aaaattcttg gttgttgtat tggtaaaggt
    12781 gcacagatag ggaactctgt tgaatttgtt cataattctg taggcactgt gattcattct
    12841 gacacaatcc ttgaagatgg tgttaaagtc tatcagaatg tgacgtgtgg ttgagcagat
    12901 agtaatgaac gtatcatcat tcgagtaaaa aagaacgctg ttttatgtgt gggtgctaaa
    12961 attctttgta aatctaacac agaattatgc gttggggaag gtgctgtagt tgctgcaaat
    13021 acagtggtat tgaggtctat tccaccctac gaagtgtggg gggtacctgc taaaaagatg
    13081 gggagataaa acatgaatgc atataaatta tacaaagtag aacgatggtt ttacttacac
    13141 aaaatggagt tttttgccaa gattgtaaag gcactaattt atgtacttca taatagctca
    13201 atttcatatc aaacagaaat tggagataat tgtaagttct tatacggtgg cataggttgt
    13261 gtaattggaa aagatacaaa aataggtaat cacgttataa tcggaacgaa tgtactgact
    13321 ggtggtagat ccaataagcc tggtatgcct acaattggaa acaatgtata tatcgctact
    13381 ggcgcaaaaa ttttaggtaa tatcacaata ggggacaatg taatagtagg ggctaatgct
    13441 gtggtcatta atgatgttga acctaattgt tcggtaggag gcgttccggc tagagtattg
    13501 aaaaaagata ttgatgtaaa tgattattgt agtttgaatc gttactaaaa ggagagattg
    13561 tatgttgtat tttgttattc ctttaatgag tgcgaaggca gcaaacaatt gggagatggt
    13621 tagtacctta tttaaccgaa cattatggtc gtgttataat cagactgatc ctgatttcaa
    13681 aatcattgtt gcttgccatg aaattcctaa actcacgcat gaatatgatg acagagttga
    13741 atttattcaa gttagcgaaa aggatgcccc tattcccaca aatcaagacg aaaaaatgat
    13801 tgataagggt tataaaacac atgctctcgc aatgcgtttg cgagagttgg gcggcggttt
    13861 cgctatgatg gttgacgcag atgatcttgt aagttctcat ttggcggaat atgtgaattc
    13921 ccatccccag gaaaatggat tttatgtcaa aacgggatac gtgtactttg ttggagacga
    13981 ttatatgaag gttctaccaa agttctcatc aggaagtgcg tgtatagtaa aatatgcagt
    14041 ggaagacctg cctgaggcat atccggaagt tatgacagca aattgtgatg ataatccatg
    14101 gatcattaga aaacgacatg ggggcgttgt tccagcatgt gaagagcaag ggcgtccatt
    14161 aaaacctctt ccattcaaag cagcagtata tgtattagga acaggggaca atcactcgct
    14221 ttatggtaaa acgacaaaat atcagacgaa aatgcgagag atactggaaa tgtttatagc
    14281 aaaacgtaaa attaaaggaa aactgaaagc agaattttct gttgattggt tataaggtga
    14341 gtattatgaa ggtattgatg tgtgaaagcc aaatgccaga gaattggtat tcgtttgatc
    14401 tttctaaaga acttaatcaa tatgttgatt taacattgct gtgtaagcgt gatgttcctc
    14461 ctttagatcc taaaattaag agcaaacata ttttgtatca taaaagtaca aataaagtgt
    14521 tttcggcact tgtttttctg aaaagcttgt tcttaatttg gaaggaaata agatttggtg
    14581 attatgatat tgttcatttg caattgatga aaagtctcaa atatgaaggc ggattgtacg
    14641 ttttatgtaa gagacactat aaaaaattaa ttatgacagt tcataatgtg ttaccacatg
    14701 aggttcgtga aaaggatcgc caagagcact ttaatatata ttcaaattgt gatggtttca
    14761 ttgttcacaa tcgagtaaca gaacagcatt tatatgctga attcccagaa ttaaatgcaa
    14821 agtcatatgt ttgtccacat gctatctatg gagatgttaa acatatatct agagcgtatg
    14881 ataaagataa gtttgaattt atcttgtttg gacaaattcg tccatataag ggaattgatg
    14941 tactgcttaa tgcaatcaca gaacttccaa tggatgtaag aaagaaagtt cattttacca
    15001 tagcaggtca acaacatcct aaacaggata gtactgacta taaaaaaatg attgaagatt
    15061 taaaggtttc agattgtgtt ctatataagc cagagagaat ttctgatgaa gaaatggaca
    15121 gtatgttcag aaatgcagat gccgcaatat ttccatataa agaaatctat ggaagtggag
    15181 cgcttcttat ggcatacact tattgcttac caactattgt tagtaatgta cctgctttta
    15241 tggaagaaac cgaggaagga aagatagggt tacttttcga aaatttgaat tctaagagcc
    15301 tagcagacac aattgtaaag tatgtgaacc ttagcccagc tgagaaaagc aaaatagagg
    15361 atgatatata tgagcttgta aaccgcaagt atacctgggc ggcatctgca aaactcacat
    15421 acgaggcata caaacaaatt ttgaatgagt agtaaaacaa ttagaaagga ataataatta
    15481 acatgcagat tagtaaatgg aaaatcatac agtttctaat ggtctttcta atgattatct
    15541 catcaagcat attgaagcct ttgggcggct ggttgggaga aacgatgaac gttagacttg
    15601 tgacagtcat tctagcaata gtatgtttgt taatagcctc attaccagca tttagaaaaa
    15661 atagatattt aaatttctgc actttactga tttgtgttgt tataatagca gcttacatct
    15721 actcagataa gacaatatgg gttaaatcta gggaaagtat gagtttatac tatgtattat
    15781 tggcgtatcc attgttctgt actttaatta accaagcgtg gaatttggat tcaatgctaa
    15841 atacgatatg tggtgtaaca tttctttcat acattcttag aacatctatt tcacttattc
    15901 agaaattttc aggagtactt ctctatgaaa acatatatcg tgagtgtgcc ccggaaaatt
    15961 ggtatcgtgg aggcttttta agaattaatc caccatgttt tacaattata ataataccga
    16021 ttgctttgta tatgattgag cgacaagttg tagctagaag aaaagtaaaa tattatttac
    16081 tgattgctag cgcactggca tattctgctg ttattcatgg ttctagatct gtccttgtct
    16141 atcaaattat tgtacttggt tgctatatcc tttttaagaa aggttcttcg aaaagaaaaa
    16201 ttgcaatgtg ggttttagct gggttgcttg tagtcatatt tattaattcg accatgttct
    16261 caacatttgt tgaaactttc acaaacagta caggtgaata tgcaggatct gctgaatctc
    16321 gatttgcttc agtttggttt tttattccta aatttttgca atcgcctttg ttcggtactg
    16381 gtattcttac tggtgaggaa tttacgttta tattaccagg aggagttaga ggacacctat
    16441 ctgatattgg atttttctat acaattactc aatatggtgt aggtatactt atatttgata
    16501 ttctgtttat tataaaaggg ttcaaaacag cattacttgc atcaaaagtg ggtatgactg
    16561 gctatcaata tttagcattt ggaattacat taaatgtctt gctaacaggg attaatatag
    16621 actggtttta tccgatattt actccagcta ttcctgtatg cttagcggtt atagaataca
    16681 tctatcaaga atgtagaagt gtggctaaca tagaatagga aatattaata ggagtgcatc
    16741 tcatgaataa gaaagagatt ttatataaca tattgaagaa gtacgaaaat ttaccatatg
    16801 tgtttaggaa catgtttggt cataatataa gtaaactcgt atcttggaaa ttgactgctg
    16861 attgtgcaac agtagaagaa aattctgaag tttatgcagc gtttcaattg cgctcaaagg
    16921 acatgtctga tgttccagtg atgggtttag ttaattgttg caaagatgta gcaattgtaa
    16981 tgcaggggcc actatgttta aaggatgatt tctcttataa tactctggcc tactataaga
    17041 aatgttatcc aaatgcttta attattcttt cgacatggaa ggacgaagat attaaggcat
    17101 tgaaaaggat agaggatctt ggagttatag ttatttgtag tgagaaacct gaaaagcctg
    17161 gacacttaaa tattaattat caagttggaa acactttgcc tggaatagtc agagcaaagc
    17221 agcttggtgc aaagtatgtt tgtaaaacta gaacagatca aagaatatac catccaaatg
    17281 caatggcgtt tttctgcagt ttacttgaac agtatcctgt taataacgaa gatgattgga
    17341 aattaataca aaatcagcga ttactgttac tatcaatgcc gtatggagac atgtttttcc
    17401 cgtattgttt gtcagacttc ctgtattttg gtgatgttga tgatatgata aacctcttct
    17461 cgattcctag tgatgtgaga gaaaagggag ctgtgtctag aggagtctca agaagggaga
    17521 tatctgaaaa taatttagct ccagaagttc agcttcttcg aagctatatt tcacgaatgg
    17581 ggggcaatga ggagtgctct attagggcat attgggagtt tgtaaaaaac catgttatta
    17641 ctattaacaa aaatcaaatt gatttgtact ggcataaata tgtaggtcgt tattcaaata
    17701 atactattta tggtacgtat tatattgatg atagccaaga tgcacttcac tgctataatt
    17761 ttgattttat aaattggttg aatttgtaca ctggaaaata cgaatacaaa gcagaatatg
    17821 aaaaatatat ggactttgtt tgggaagagt aattaacttt tatagtaaaa agtactttag
    17881 ggaagggcat attatgaaaa cagtaattat ggcgggcgga cgaggaagcc gaatttcgga
    17941 attgtttccg gatattccta agccaatgat tcctatagat ggcattccag ttttagaacg
    18001 agaattgtgt tcgcttagag atcaaggctt taatgatatt attttgactg tttcctattt
    18061 gcatgaaaaa atagaggagc atttcggaga tggttcaaaa ttaggaatta aaatcaaata
    18121 ttttattgaa aaaactccac ttggcaatgc aggtgcattg tattttttgt tggatgaact
    18181 gggtgatgaa ccgtttttac ttttgaatgc tgatgcagta tttgatgttg atttcaggca
    18241 gatgattaga tttcatgttg agaataacgc agatgttact ttgtttacgc atcctaactc
    18301 acatccgtat gatagtggat taattatatc cgatgacaat aagaaagtgc ttcaatggtt
    18361 agcgaaggaa gatcaaagac ctgaatatta taaaaaccga gtaaatgcag gtcttcatgt
    18421 tataaatccg aaggtgttga gagaaaatcc attaatggaa attgtaaata aatcaatagt
    18481 tgaaggtggg gggctaccat acaaaattga tttagataga atgattctga aaccattggc
    18541 taacacaggg cgtatgtatt gctacgattc tccagagtat gttaaagata tgggaacacc
    18601 ggaccgatat agagctgtaa ttaaggactt tcaaaaggga attattgttg gtaaaaatct
    18661 atccaatcca caaaaagcaa tctttttaga tagagatgga acgattaata aatacgttgg
    18721 ttttttgaga aatgcagaag aatttgagtt aattgatgga gtatcggagg caattaaaat
    18781 tattaattct ctaggttttc tgtgtattgt tataacaaat cagcctgtaa ttgcaagagg
    18841 tgaagttact gttgatgagc ttaatagtat tcacaataag cttgaaacaa tgctagggca
    18901 tgctggtgga tttttaaacg gtatttatta ctgtccgcac catccccata aaggatatgc
    18961 aggagaggtt atagaattaa aaattgattg caattgtcgc aagccaaaac ctggactgtt
    19021 attagaagca tcaaaggatt ttaacataaa cctaagtcaa tcatttatgg ttggagatag
    19081 tgaaagtgat gtaattgctg gaaaaaatgc tggttgtaag acagtactga ttacagaaga
    19141 catcaccttg cctgctaatt atggccaaga ctatagtgtc tgttcattgt tagaatttgt
    19201 taaaaatcat ttatgcacta tgcaataatt ggtttagtga aacttgtaaa aggagtaatt
    19261 agatgaaggt attaattcca gcagcaaaag ttgttgaaaa agaattgcag agtataggaa
    19321 tgttaccagc aattatctat ccaattaata aaaaaattgt ctttgattat ttgtataatc
    19381 agtataatgc aatctgcgat tcattggata ttatctgtta tgaaaaagcg gataaggttc
    19441 atagaagact gaaaaactat catgattcca gaattcatat atgtgacctt cctgaattag
    19501 gggatctagg gcaaaccgtg tattttgggt tatcagatga agatgagcct gttctaatta
    19561 acttcgcaga tacaattgtt atggagagta tgtctgatat agaaatggat acattctatt
    19621 attctgaaga ttttgtgtcg agtacttgga catactttaa aattaatgaa ggaataatta
    19681 ctgatatcca cgataaagag gaagcttacg tatccgataa acgtgaaaaa ttatttattg
    19741 gcgtatttat gttttcttcg tcaaaacttc tgaagagttg cttaagagag gcagttgaga
    19801 gtcggaaaca ggggggaata aactcatttt atcgggcttt acagctatac agcgaacgtt
    19861 gtccgctaaa ggccgtaaga acagaaaagt ggtttgatat tggacacatc gataaatatt
    19921 acaatacaaa gttggaagtt aaagcacgag agtttaatca tatctctata gataaagatc
    19981 gagggatatt gaagaaaact agcgatgaca aggataaatt tattggagaa ataaagtggt
    20041 atttaaaact tcctgctgat attgaatatg taagacctag aatttttgat tattctacat
    20101 cttacgtagc tccctatgtt gatatggaat attatgcata tcatacggtt catgagttat
    20161 acttatatgg tgatttaaaa tataaccagt ggcatgatct gtttagccgt ataaagttta
    20221 tctgtagcga ttttaagcga tacaccttaa aagatgaaaa gattagagag tcattggaag
    20281 atatgtattt gcgaaaaacc atgcagagat ttcaaaaatt gtctgaagat aaaagatttt
    20341 cagctttttt tgagaaacaa attcaaatta atggtcgtaa gtttatttct ttaaccggtg
    20401 taattgaact tctaaagaat cttatcccaa gtatgctcta tgatgtaaat gaattttgta
    20461 ttatacatgg agatttgtgt tttgctaata tgatggtaga ttccaattgt tcctttgtta
    20521 aggttattga tcctagggga aaatttggaa aatatgatat ttatggggac agcagatatg
    20581 aattagcaaa actatttcat agtttagatg gtaaatatga ttatatcatc aaggatctat
    20641 ttactgtaga atatgatacc gagtgtgcag tgatagatta tacaattaat acgcgtgtaa
    20701 gagattacga tttatttgaa ttgtttaagt cggtcttttc ggacgagatt aaggacgatt
    20761 ttgataagat tgtattgata gaagcgttgc tatttcttag tatgattcca ttacacggtg
    20821 aaagttttga tcaccaaata gtgatgcttg ctacaggtat ggaacttctt gcaagagtaa
    20881 gtaataaagt tatactctaa tataaattgt gccttaatga ataacggtta caatactgtt
    20941 ttagcaatgt aggcattaag gcacgattat attaattaat acaatagaag ggaagagaaa
    21001 aatgtatagt gaatattctt ttgtgtttga tatcgatggc actttgtgtc cgataaagaa
    21061 gaaggatgag cgatatgagg atttggtgcc gtatgagaat atggttgaaa aactgagatt
    21121 ctataaggag aatggtgcta aaattacatt gttcacctca cgcaatgtta attcctataa
    21181 aggaaatttg ggaatgataa atgctaatac tgcaaaagtg ctcttggcgt ggttggataa
    21241 atgggacatt ccgtatgatg aaatcctgta tggtaagcct tggccggggc ataaaggctt
    21301 ctatgtagat gatcgaacgg ttagacctga tgagtttttg agatgctctg tagatgaatt
    21361 aaatgaaatc tgcaacagtt caaaaaaaag attctaaata aggaggttag ttctatggaa
    21421 aaaaagatga cagttatcat tacgatggca ggtttgggaa gtcgttttcg aaaaattggt
    21481 tatgcggttc ctaaatatat gattaatgcc aaaggaagaa ctttatttga ttgggccatg
    21541 gatagtttgg ttggatacaa taaaaatgtt gataagtata tttttgttgt tcgtcaggaa
    21601 gacaacgctt cagcttttat aaaggagcat tgtgcttact ttggtattga aaacgtagaa
    21661 gtgattgaaa tagactatat gacagatggt caagcaacta catgtatgtt ggctattcct
    21721 tattgcaatc aagacaatga gattatggtt tacaatattg atacatacgt ggaatcaaat
    21781 gagatgaaat acgaagatat ttgtggagat ggacatattc catgctttca tgctgatggc
    21841 gatcattgga gttttgcaaa gcttaacgat cgaaatgaag taatagaagt ccgcgaaaaa
    21901 gttcgcattt ctgataattg tactcttggt gcgtattatt tctcatcggc aaagctgtat
    21961 aaagctttat atgaagagta ttatgcagac tcctctaata tggagaagaa tgaaaaatat
    22021 atagcaccat tatataactt tatgataaaa aaagggttaa aggtgactat tagcatagta
    22081 gatgcgaaaa aggttcatgt gcttggtact ccagaagagc tcaatgtgtt tttgaatcaa
    22141 gaataaatag tgtttaggag aaaatagcag tgaccagtaa taatctaatt gtaaaaacat
    22201 taagcagata taagaatact ccacttcctg ttaaggctgc attatggttt atggtatgtg
    22261 gttttttaca gagaggggtt tcaatgatta caaccccaat ttttactaga gttttgtcta
    22321 cagaacaata cggtttatac agcacttatc tatcgtggga atccttattg ctcatgatta
    22381 catcgcttag tttgttcaaa gccatgatga atctttatgt aaaatatgat aatcaagaac
    22441 atattctttc gacagtatcc ggattgactt tagtactgac tattttttgg tttgttattg
    22501 ctatttttat taaggatata ttggcagaaa ttttaggatt gacaccagtt ctagttgtct
    22561 gtatgttctc atattttata tttcaggcag aaatacaatg ttggtcttta cacaaacgat
    22621 atatatatga ttataagact ttattagtag ctacccttgc agttactgtt ggctcatctg
    22681 tagttggctg tttagcggtt ctgtttatat ctcctaccgc tgaggcgaga gccttaatga
    22741 atgtcctagt gactggtgtt gttggcgtgg ctgtgtatat atcggtattt agaagatgta
    22801 aggattttaa ggataatgaa gtgtggaatt tcgcagtagt attttgtgta tcgttgatgc
    22861 cacactattt atctgaattt attctgaatg gttctgataa aattatgata aattacttat
    22921 gtggttcgtc agatgttgct atttacagcg tggcatattc tattggtgct ctaattaacc
    22981 ttgttacaaa ttctgtcaat tccacttttg caccttatca gtatcaaaaa atcaagtcag
    23041 gagaatatga tttactatca aaaagagcta atgaagttct tggtttggta gcggctatgc
    23101 tttttgcatt aatgctattt agtaaagaaa ttgtgttgat atttggtggg cagaaatata
    23161 tcgacagtgt taatttaatt gtacctatat gtatcggtat ttatttcaac tatatgtttc
    23221 agctgtttgc tagagtacag gaatactttg aaaagaaact cactgtcgtt attccatcgg
    23281 ttttgtgtgc aattatgaat attgtactaa attatgtttt tataaagtta tatgggtatc
    23341 aggcggtagc atacacaact gtattctcat atatgttttt ctgcgtcatc cattatattt
    23401 tttatagaaa agtttgcaaa gaactattgg atggaaaaaa catatatgat gtcagaggaa
    23461 tacttttgat ttctgcaatc acgatagctg gtggcatcct aatttcaata attaataaat
    23521 ttgatttcat aaaatattta attatagcaa ttgcttgtgt cgctgcaatt atatttagaa
    23581 aacgattgct acttttagta aggagaatta tgagcaaata aacatttgct catagagaga
    23641 tagagaatga tagatattat ttctgctata acggtaaatg tattaacagc attatatcag
    23701 ccgttcttat ttgccgtatt aacagcgtat ctgtttatgt actggtatct atttatagtt
    23761 gataactctc gagatcctaa aaacttaaaa gattctgtat taatctggtt gagatatttt
    23821 aaagcatcta gtaaatttcg cagactcttt tttcttgcgt tttattttac tttgatatta
    23881 tttaaaacct tgcttaatcg aaatatgtgg atgaacccgc tttcaaacgt tattggacag
    23941 tggtcattat atattataga taatgctaca ggagagagca gattgacaac agaatgcgtg
    24001 gagaacacga tgctgtttat ccctttttgc atgttattgt tatggtggcg tgacattaaa
    24061 tgtgatatta tacgcaccat ctatgttggg attaagtatg tgtttttatt ctcattaagt
    24121 atagaattta cacaactgtt ttttaggctt ggtacatttc aactatcgga tttgttttat
    24181 aacaccctag ggggcttaat tggtgctttg ctatattggc tattctaccg attaaataag
    24241 tatatagact taaaatagaa ctgctttgaa ttttaatatg gttaatattt ttctgcagac
    24301 aggattgtgt aatctcatca agaatacttt acatgccatc tatttttgct aaaacattgt
    24361 tgttatatct tcaagaggta gggaatttta ttgctttaaa tgaggtcgag cgaattaaaa
    24421 aattggtaaa atatataaca tggtggtagt tatggtaaaa gtgtttgata taattaagtt
    24481 ttcggatttc agaggatata ctgctgttgc attggataat gagactcttt tctcgattgg
    24541 tattgagttt cacattaagc aaataaatca aggatttagt cataataaat atactatgcg
    24601 agggctacat tatcagagtg gagagcatgc tcaaggaaag atagtgatgt gcctacatgg
    24661 tgaaattatg aatatcgcac ttgatattcg acaaggatct tccacttatg gatgtgtgta
    24721 tgtcaacaca ttgagtgaaa gcaatggtaa atgtgcgtat atcccacgtg gatatgctca
    24781 tggctatctt actatggagg acaatacgct agttcagtgg tgcgtagata atgatttcaa
    24841 ttctaatgct gccaaatgta taagttatga aagcgtagat attgaatggc cggtcagtcg
    24901 gcagtgtttc gttataagtg agaaagacaa aaatgtggaa agattaagct tttgattaaa
    24961 atgagctgtg gagaaaagaa ggatactgga aaataagaag aacaataacc gagcaaatgc
    25021 tcggttattt ctatgaggaa tacataaaac taaatttaga tataga
//
LOCUS       DACECB010000040        24788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECB010000040 DACECB010000000
VERSION     DACECB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.040"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(3..2060)
                     /locus_tag="IWO04_001684"
     CDS             complement(3..2060)
                     /locus_tag="IWO04_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0213296.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2174..2386)
                     /locus_tag="IWO04_001685"
     CDS             complement(2174..2386)
                     /locus_tag="IWO04_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213297.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2573..3040
                     /locus_tag="IWO04_001686"
     CDS             2573..3040
                     /locus_tag="IWO04_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213298.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3037..3279
                     /locus_tag="IWO04_001687"
     CDS             3037..3279
                     /locus_tag="IWO04_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213299.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3297..3599
                     /locus_tag="IWO04_001688"
     CDS             3297..3599
                     /locus_tag="IWO04_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213300.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3671..5725
                     /locus_tag="IWO04_001689"
     CDS             3671..5725
                     /locus_tag="IWO04_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213301.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            5725..8337
                     /locus_tag="IWO04_001690"
     CDS             5725..8337
                     /locus_tag="IWO04_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0213302.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            8334..10712
                     /locus_tag="IWO04_001691"
     CDS             8334..10712
                     /locus_tag="IWO04_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213303.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            10773..11315
                     /locus_tag="IWO04_001692"
     CDS             10773..11315
                     /locus_tag="IWO04_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213304.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            11393..11725
                     /locus_tag="IWO04_001693"
     CDS             11393..11725
                     /locus_tag="IWO04_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213305.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11726..12319
                     /locus_tag="IWO04_001694"
     CDS             11726..12319
                     /locus_tag="IWO04_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213306.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12341..14035
                     /locus_tag="IWO04_001695"
     CDS             12341..14035
                     /locus_tag="IWO04_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0213307.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14723..16600
                     /gene="ltrA"
                     /locus_tag="IWO04_001696"
     CDS             14723..16600
                     /gene="ltrA"
                     /locus_tag="IWO04_001696"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0213308.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16731..17108
                     /locus_tag="IWO04_001697"
                     /pseudo
     CDS             16731..17108
                     /locus_tag="IWO04_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            17108..18313
                     /locus_tag="IWO04_001698"
     CDS             17108..18313
                     /locus_tag="IWO04_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0213309.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            18329..18973
                     /locus_tag="IWO04_001699"
     CDS             18329..18973
                     /locus_tag="IWO04_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213310.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            18984..19790
                     /locus_tag="IWO04_001700"
     CDS             18984..19790
                     /locus_tag="IWO04_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213311.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19812..20042
                     /locus_tag="IWO04_001701"
     CDS             19812..20042
                     /locus_tag="IWO04_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213312.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20588..20863
                     /locus_tag="IWO04_001702"
     CDS             20588..20863
                     /locus_tag="IWO04_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213313.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            20905..21327
                     /locus_tag="IWO04_001703"
     CDS             20905..21327
                     /locus_tag="IWO04_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213314.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            21346..22938
                     /locus_tag="IWO04_001704"
     CDS             21346..22938
                     /locus_tag="IWO04_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213315.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            22950..23285
                     /locus_tag="IWO04_001705"
     CDS             22950..23285
                     /locus_tag="IWO04_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213316.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            23432..23626
                     /locus_tag="IWO04_001706"
     CDS             23432..23626
                     /locus_tag="IWO04_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213317.1"
                     /translation="MTQTTISVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            23658..>24788
                     /locus_tag="IWO04_001707"
     CDS             23658..>24788
                     /locus_tag="IWO04_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213318.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT         9131 a         3866 c         5031 g         6760 t
ORIGIN      
        1 ttttatttga agattggatt cgcactaata aatacattat ctacgccaat tctaccagca
       61 gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata aagtaaattt
      121 gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc atcgtctgat
      181 aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc accgtagcag
      241 cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg actgaagctg
      301 acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt gaattgcatt
      361 ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt ttgacgcata
      421 aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt tttattttga
      481 tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg atcgtcattt
      541 ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa gctatcccct
      601 tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt tacatgatta
      661 acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc agtcacattg
      721 tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc ttgaatagaa
      781 ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc tttcaaaata
      841 ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg atgcacttga
      901 ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt atcattacca
      961 atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc ttttgcctta
     1021 gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac ttgacctgtt
     1081 tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg tttgtgttgc
     1141 aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt ttgagaaacc
     1201 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     1261 tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca
     1321 gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga atctaattta
     1381 tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac tttcttagca
     1441 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt
     1501 cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg tttagaggtg
     1561 tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt
     1621 acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg
     1681 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag
     1741 ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
     1801 agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc ttcccaggcg
     1861 gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt tttgctagag
     1921 gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt agttgaagca
     1981 gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc taaaacagtt
     2041 tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg tttacatgat
     2101 tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt atataatttt
     2161 ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt
     2221 tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa
     2281 ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac
     2341 cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc
     2401 ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt
     2461 tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt
     2521 gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa
     2581 aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc
     2641 aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg
     2701 gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga
     2761 atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga
     2821 ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa
     2881 caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat
     2941 ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc
     3001 agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct ttttatttat
     3061 cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc cgcgtctaaa
     3121 atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct tgaaaaagca
     3181 caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta tcaacgtgaa
     3241 cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat aatcttatgt
     3301 ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca gttgatattt
     3361 ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg
     3421 ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag
     3481 ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg
     3541 ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa
     3601 aaaaacaagt aggaggagta aaaagtacgt ggctactttt tactcctttt tttatgaaag
     3661 gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag agttaataag
     3721 aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc
     3781 tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac aagagaatca
     3841 atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg
     3901 cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt atcctacatt
     3961 gatagattta atccgccttg atgtgtttaa agatacggat gaaactatga gtgtggaaga
     4021 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     4081 aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     4141 ctttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     4201 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     4261 tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg atccagttga
     4321 agctaatgaa gagtcaacat ggactgaagg ggattttcag gaatatattg aaccacttat
     4381 cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat ttgattatat
     4441 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     4501 tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag agagtaaaga
     4561 agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg tccaatcaac
     4621 aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac gtcaacaaga
     4681 aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg agaaagtaaa
     4741 agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa aagtcaaaga
     4801 gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt gtagaaaaga tcaacaattt
     4861 tactaagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa aagaacgtat
     4921 taaagaacga aaagaagagt tctaccgcgg tttacgtaca gataagctaa agattagcaa
     4981 agaattaaat gaacaactga ataagctata tcaaacaacg actgaagttc ttatggaaaa
     5041 acacgaacag atccagcaag aattacaagt acaaattcca aaatggaaaa aagagtatga
     5101 aattcaacaa caagaattga aagaacaaga agaaaaacaa cggcaaaaag aaaaagagcg
     5161 tgaacaatta gaaataagaa aacgggaaca ggatcttgcg gaacaaaaaa tggcgataga
     5221 aaaacaacgg ttagagcaag aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga
     5281 acaaatggat acattgcgtc aagcacagtt gaattatcta gcacaacctc aaccagtagc
     5341 tgctgttcaa acgccagtga acaaccaagc aaatttaact ccttcacccc agcgttcctt
     5401 taacggatgg atggtgggat gtattgcaag tttaagcttg cttctaggtg gaggaacagt
     5461 atttgctttt aatcatatca atcaatcttc acaggctgat tctgtagcca caacggcgtc
     5521 aatcaaggaa gaagcccagg aacaagcaag aaaagagtat gaacaacaat tagaagaatt
     5581 attgaatcgt accaatcagt caacaacaga gtcaagcatg gaaaatagca aagagagtga
     5641 ttctagtaag gctaaagaag gatcaagcga ttcaactgta caatcaaaca cttccgaaag
     5701 ttcagaatca aatgaggtga aataatgcgt gttagagaaa caacgaagcg aaaagacaaa
     5761 cgattcaatt tagaaaagaa taggtatctt tctcatttct atcgctatac tccctcagga
     5821 gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa agatttttta tagtggactt
     5881 gtatacctcc ctttagagta tttagtaaca agagtaccag aagttttcaa ccgattggaa
     5941 attgttaagg gaagcttaca aggaataaat ctgtttcaat tacaggaaat gccacaggtc
     6001 tttcattttg attcagctgg tttttggaat ccatttactc ctacaactag tttgggtaca
     6061 cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac
     6121 atgtggtttc gtcctctagc agatggacaa aaaggggata atcgattaat gaccttaaaa
     6181 gaaatccaac agatttatcc gcaaatacct gaaacaaaaa ctacttttcc aggttatgga
     6241 ggtattccaa tcacacatta caaaaaatat tggtacattc aaacagatac tgttaatacg
     6301 tgtattgttg gtacctctcg atcagggaaa ggtcagattg aagtgttagc caccattgac
     6361 aatttatctc gtgcagaaaa acaaagttca atggtcgtta atgatccaaa aacagaatta
     6421 tatgttggta gtaaagatga actagaagct agagggtatg atgtatatgc ctttaatatc
     6481 accgatccac tgcaatcaat gagtgataat ccattggcac tcattgtgaa atactggaag
     6541 cgaggagaca tagacaccgc aacacagtta accaatactt ttacgaatac gatttattat
     6601 gatgcaaatg cttcagacaa taaatacttt aacgaaaatg cgcagaaagc tgtcaatgcg
     6661 cttatttttg cgttattgga acaagcggat caaacagggg attactccaa attaacacca
     6721 aataatctcg ttgaactact ttcagaaatt ggcggtttta attattatcc agatcctgag
     6781 aatgaattca agcagctcaa tgccttagat gagttcatga atcagttacc ccctggaaat
     6841 atagcaaaaa aacaatatgg ggcaacaaaa attggatctg ataaagcgaa agggaatatc
     6901 ttgtctacgg caattacagg attgaatcca tttaccctaa cgaaaattgc gaagatgact
     6961 tctcaatcga ccattgatta caagtccatt ggttttccga agtatttgga tctcaaatta
     7021 catgaaagcc tattaaatca acgcattgtc gtggaatttt atcataaggg aaagaaattt
     7081 cacgaggaac gagtcaaagt tggctatcgt ggtttttgtg aaatcaactt tgattgtcat
     7141 ttagagcagg gagatcatgt ttgcttaaaa tacaatcatc aaggaaaaca atatgttgct
     7201 tggtatgagt tttctcaaaa agtattgaaa gacgaagagg gaaaaatcgt ttatcgaaaa
     7261 aaacgaggag aaactcattt gccagaacta gaaaaagaag ccgttctaac attagatgag
     7321 aagaaaagta acctattagt agagtctatt cggatgcact ataccgataa accaatggct
     7381 atttttatgt taacgcctga ttatgatcca tctaaccata tcattgttag catctttctt
     7441 agtcagttgt ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac
     7501 cgtaggattc attttatttt agatgaattt ggaaatatgc caccgatgga caacatggaa
     7561 ggaatcatga cggttacggc aggtcgtaat atgttatggg atctgtttat tcaatcttat
     7621 cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga aaattgtcag
     7681 acacatattt atattatgtc gacagatgat aataccatcg aagaattttc taaaaaagtg
     7741 gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat gaatactcat
     7801 atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc tactttttta
     7861 gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg tcataaaata
     7921 cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta tcaatatatt
     7981 gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac acatgcggac
     8041 ttggatctta aggctttaca aattgattat gattatttcc taccagatga atatgctaga
     8101 aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat
     8161 aaaatcgttc aatcttttga gaatgagacg gctaaaaaat tgttaattca attttacgaa
     8221 gaacaagcaa cggatcgtct tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt
     8281 tctgaaatga ttaggaaacg gctgttattg gaagctgaaa aggcggtgaa agaatgatca
     8341 ttggaggaat tttctctggc atagccgact ggtggtcagg tttattcgga gaaggggatg
     8401 cagatcccaa aaccgtaacg aaagtcttac aagattatgg taattatctg cagtatcaag
     8461 atgtattcag ttggatttgt aatacttttt tatggggatt gatcaaaggg ttatataatc
     8521 tcaatacttt attagaaaaa acaatttatc aaagctttga tttaaaagac ttattgaacg
     8581 ccgctggtgt taatgaacta tttcaaagtt tgattagtaa ggtattggct cttttttgtg
     8641 tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca ccgaaagtca
     8701 aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggt agttctttaa
     8761 tcgatgaagg gttaaatatc agtaaaaact tttatggaga cgtcactgca gcaaataaag
     8821 cagataaaac gggttcacct gcttttcaaa ttattcaaaa taatgtgatt gatgtcagta
     8881 cattactagc gactgacccg aataaagtag aatccttacc aacagataaa agaaatgctt
     8941 taacagcgaa aaatttcaaa acagcgaata tcaatgggat tattacacct gatcaagcta
     9001 agaaaatgtc tgaatcaagt gatattagta cagtacaaaa agatcggtta aaggcattac
     9061 agtatcgact agagttagac gataatggga aagaaatccc tgttcaaatt agtgatgaag
     9121 gtttagcaaa atatgtgtat acgtcaggct atcgtcgata cattagtagt ccagggatta
     9181 ttttggctgg ggaatcttct ttgtcggtag cctatttatt tattttgttt acaatcgtta
     9241 cttgcattat tgagctagta tttaaaaaat tctatttagt aattgccgcg tcaacggact
     9301 ttgagacagg acaacggatg aaaagtgcga tagaagatat ttctcaatcc ttcttgttat
     9361 tggcgtttac gattttagaa ttacgaatct atacattaat gctttcaggt atcggtgatt
     9421 tgcatgcggt aggaaagatc aatggattct tgtatgttgt tgctttaatt gtgctaacta
     9481 ttgcgctatt caaaggctct caatcggtta cgaagatttt cggtgttgat acgagtctga
     9541 aaaacggcag caattctctt cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg
     9601 gtagcggagc taaaaacctt gcaagtgctg gtaagagtgg tttaagccat ttaaatgata
     9661 ttcgtaagaa tggatttggc catcgaaaag atattaccca tgataacggg gaagaacaat
     9721 cagacgataa agctacaact gtacctaata gtaagaagac agtttccgaa atgttcggga
     9781 aagcgaaaaa tggtgtttct aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt
     9841 taaaaggaac agttgaagat cttacagaaa aagccaaaga caccttagaa gagaaattag
     9901 acggcacgag agctaaagaa tttgctgaag ctgtgaagaa tccatccgaa gcactagaaa
     9961 aacaaaaaga aaaagcttca gcagtcaaag aaggactaaa agatgcgtat ggtgaaggac
    10021 aagtcaatgc agcagtgaag gcaaatacaa caagtgaaaa agaaaatcaa gaaggtgcag
    10081 cgattccttc agccaaagat caccaagatg agttacattc taacgaaaat tcgatcggag
    10141 aaaaagattc agaagaagga caacatggtc agttaccaaa aaccgatcca gaacaatatc
    10201 gttataaatc taacattaat tctgaaaacg aaaaacgcat ttcagattta ccaaaacgaa
    10261 atcaagaagt acaaacggat gaggattcca tcgatctgcc aaatgataat ggcatcgaag
    10321 ttggaactga aaaatcaacg aaaattggtt tgcctaagac acaaaattta ccaatgaaag
    10381 atagtcgttt gaatcaattc aaagagaatg atcaaagctt tgttcatcaa tcacctgcag
    10441 gtactgttca aatgaaatca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    10501 acaatttagc tgaaagtggt gaaacagaga atgcgtccgt tcaaaatatt gtgacaaaag
    10561 aagctgcacc taagccaatc aataaagttc ataatggata cagaccagca aacgtagcat
    10621 ttactgcacc gacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgaattga
    10681 aagaacaact attgaaggat atcaaaccat aaaaggccta tttaagggcc tttttcttta
    10741 taaaacaagt ttaaaatgga aaggagaaaa ttgtggagga tattttaata gctacgttta
    10801 taactgcact gggagcattt ttaacgatat ttgtggtgac tattaaagga tttaaagata
    10861 cggatagttt acaaaaagat catcagtcta ttttagatgg tcaaaaaaca cataccgaaa
    10921 gcattattgt acacctttca gaaggattca cttatttgaa agatgctatg cccacattca
    10981 cagtaaaatt aacggaaata tttcattctg ttgagcaaat atcgtcagaa ttgaaagagc
    11041 aaagacaaca aaaagatcgt caacttggct cacttaattt taggcaagaa acgatacaag
    11101 aatcattgga gaatgtaaat cgattaaacg atgaaatgca acgagttcat ttggaaaatg
    11161 aagaattacg cgcacaaaat tatgatttaa ttcaaaagaa aagaaatctg gaacaaaaaa
    11221 ttgttgaatt aaaagaagaa aatcgtcaat tgaattatca attacgacga ggaaaaaatc
    11281 gttctcgtga tttaggaaat gaattagagt tgtaatattt agaaaaacgc tgtgagaaat
    11341 cacagtgttt tttatttcta aaaatacaaa atataagggg tatggttata ttatggataa
    11401 atacattcat atgaaaaatc ctccaagatt tagtggggaa tggaagtttt tcgccttttt
    11461 ctttttagat ctattaacgc ttggtggctg ctttttttgg gcgaaaatag tcaataatct
    11521 tgtccactta ccactctttt ttgcactctt gaattatgga ttatggcttg cgatagcgtt
    11581 agtgattgtt tggcgaccct ataataaccc tggtcaacgc caaatttttg cgattatcga
    11641 atctattttc tttacggata agaaccacta tcatgcgtta gatcctaacc gaaattatca
    11701 tggtacgtat aaggaggatg aataaatgcg agtcaaagga caaaattcag ttgataagaa
    11761 atccattttg cagtttggaa aaccagaaga gcgctctatc ttggacttat ttccatataa
    11821 atcctgtaca gaagagggag tcttaattac tagagaagat cattttcagc gttattatcg
    11881 tgtcgtttcc actgacgtgg agggattaaa tgaacaagag aaactagaac gaatgaatca
    11941 gttgaccacc gtcatgcgta cgtatgtgcc aacaattaag ttagttagtt tgaccacaga
    12001 gacggatcta acggaacaaa tcgtgcaaaa gcggcagtta ttagataaaa atcgtatgga
    12061 acagtcacta ggaagaaatc tacagcgatt aagaaaatat gaaaagaaat tagtagagga
    12121 gatccaggaa ttaaaacggg cagaacaaga acgtcctgac ttaagcttct ttttcgttat
    12181 tgaaggaaag acaatcaagg atttacaaac tagaaatcgt cagttaatgc gatctagtgg
    12241 cattttaggg ttaaaaccac tctacaaaaa agaattgatt aagattcttt atcgaatgaa
    12301 caacatgaat gacgaataaa aaagatggag gaaatgattc atgctagatg tgataaaaac
    12361 agctttttca acgcaaagta atcaaaaggt gattgatcca ttaaaacaac ttggtaatcg
    12421 tgaaatagag attttaaaat caaaaggtta tgatttagat ttcttaaaac aagtgatgcc
    12481 acaaggtggg ttgaattttg atgaagaata tgtgacctat ggaaatggtt attcacgatg
    12541 tgtgacagtt tatcattaca caaaaaatcc tactcttttt tggttggcca atctcatgaa
    12601 caatcctcat acagtggcca cactagatgt tagcacagat gaaaagcaaa aagtgattga
    12661 tcaaatcaac cgcgcattag atgaattaaa tgatcgatca gataatgaac gaaaagcgac
    12721 tgaccgaaat aaaagtgcgg aagaatacct ggaactagcg gaatatgccc gaaaattgac
    12781 ttctaatggg gaaatttcta aacaagtcaa agtgagagta catgtttacg gaaatacgat
    12841 ccaagaagtg gatgaacggg cgcaggaact gatcgaaaat ctaaaaggga tggattatcg
    12901 tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta ccatgttcga
    12961 tgaacaagct gaatggtttg gaaatatcaa agggcaatca cttccatcgt taaacattgg
    13021 cggaggattc ccgttccacc accaaacgct aaaagatcca ggaggcacac aatatgggga
    13081 aaccatgaca ggcggcccat ttgtctggaa tgccacgtat aaaaatagtg tacgtttatc
    13141 taccaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat
    13201 cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg gaaaagactt
    13261 tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat ctgacggaat
    13321 gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca gtttatatga
    13381 cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga
    13441 caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga tgaaagcaga
    13501 atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac gcggctttac
    13561 ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc cagaagctta
    13621 tccgacgttc aaagaatttt tagccttttt aggacaactg gaactaccag gggcttccca
    13681 agagaaaaag aatcggaaag aagaaatgga gtctatcgtg gaatccttat gtgagactta
    13741 tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac agttggtctt
    13801 ttttgacatt gactctatta caggcctaga tcgaggggta caacaatgtc agatgtacat
    13861 ggccatgacg ttgatttgga atcaggcatt gattcaagga cgaagacaac gacgcttaat
    13921 taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga ttgatgagtg
    13981 ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact
    14041 attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa agggcttcca
    14101 ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga agctcggtga
    14161 agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa gttctctaga
    14221 ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac
    14281 aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta gaaatgggag
    14341 agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta
    14401 tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc tagcatctaa
    14461 gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg agagtgcata
    14521 ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta gtgatggtga
    14581 aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg ctcgattcgt
    14641 ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa aaggtcacga
    14701 gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg
    14761 gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca aaagtttgaa
    14821 gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca ttgtcctagc
    14881 atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg gagtaacgat
    14941 taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga agcgattcgc
    15001 acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg gtaaattaag
    15061 accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct tacaagtatt
    15121 agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc caaatagatc
    15181 agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca tggaatatgt
    15241 ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac tcatgagaca
    15301 aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga aaatgttaag
    15361 agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa caccacaagg
    15421 cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact ggtggatagc
    15481 aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga aaaatggagc
    15541 ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa aagaaatata
    15601 cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg aagcgaaaaa
    15661 agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa tatctgaaga
    15721 aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa
    15781 attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa aagcacaaag
    15841 catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa
    15901 agataccttt atctatattg gagtctataa cagtatggtt attgggattc ataattactt
    15961 caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag
    16021 ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga
    16081 aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt ttccaatatt
    16141 accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata ttaataaata
    16201 caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag aatggaagat
    16261 atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat ttgcggacaa
    16321 tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat
    16381 cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa gtgatagcta
    16441 tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta cacaaagtga
    16501 aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag aaaaactcaa
    16561 tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg taggacggga
    16621 aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt
    16681 gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta tcgattaatc
    16741 cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg cgtaaagtag
    16801 aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa aatatgtctt
    16861 ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag atctttatgc
    16921 gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca atgacgaact
    16981 cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat tttggctcaa
    17041 aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg tttgatggag
    17101 gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc gtcgtgcaaa
    17161 gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta ttttgatcac
    17221 tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt cggattcaga
    17281 ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac cgatttataa
    17341 agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg taggtgcagc
    17401 agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg aatcagcgtt
    17461 tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt ggtttgatgg
    17521 ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg gctggtacat
    17581 gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag ccacacaatc
    17641 caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga tcttaggacg
    17701 aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag gttgtatgtc
    17761 aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac attggggtga
    17821 aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc ctggcagcag
    17881 tttagataaa agttccttca gcggtggtca attatttgga acgaatccag gcggtgaatt
    17941 ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc caggaagtga
    18001 gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt
    18061 gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc
    18121 aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat
    18181 tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc
    18241 acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag
    18301 cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa tcgtcttaac
    18361 agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc tgaaagagga
    18421 acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg agaagctcaa
    18481 aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca tcaatgagaa
    18541 aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac
    18601 tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag
    18661 cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt
    18721 aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct ttacattagg
    18781 tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt
    18841 tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa aaataaaagg
    18901 tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag actttagtta
    18961 tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa cgtttcaaaa
    19021 aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt ttgattcttg
    19081 ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc acgaacgcct
    19141 ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac cagcaattag
    19201 tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg gctaatctta
    19261 attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga gccaaagtga
    19321 ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa gaatatatcg
    19381 ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg caagaaccac
    19441 aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa gatgaagatt
    19501 atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagctc
    19561 aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta gtgaagaagc
    19621 taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa aacaagatta
    19681 atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa gaagagatca
    19741 ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag aaggacaggt
    19801 ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct gagcagaaag
    19861 tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt
    19921 tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat actttaaaat
    19981 tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct gataacaaat
    20041 aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga atcagctata
    20101 tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc taacgcaaac
    20161 atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca accaaacagg
    20221 aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca tatgacaaaa
    20281 tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac acaaataacc
    20341 ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg tgttaggtag
    20401 gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga tttgtgtgta
    20461 gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt gtgtagcaaa
    20521 atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa taaatttgga
    20581 ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata aaattgacaa
    20641 acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc aatacctaaa
    20701 agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag accaatttga
    20761 tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt tagaagaata
    20821 tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat agtattgtga
    20881 atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt atatctttac
    20941 gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat atctaattat
    21001 agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat
    21061 ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac cgaaatggtt
    21121 tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac aaattcaact
    21181 gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca aaccaaaaaa
    21241 tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt agatccattt
    21301 gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa taaatcgcct
    21361 gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt
    21421 gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc cgaagaagaa
    21481 caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact
    21541 ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg
    21601 atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa atatctttct
    21661 tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa
    21721 gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca taaaaaagct
    21781 tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg
    21841 aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat ttatcaagat
    21901 gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc caaaacagat
    21961 accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac attattcgag
    22021 aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa cactaagcat
    22081 atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta tactgagatt
    22141 gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac attagacgca
    22201 atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga tcgaatgatc
    22261 ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac cttaacaaaa
    22321 gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa tgaagtatat
    22381 aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg aaccaagctt
    22441 tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac aaaagaacct
    22501 ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa aagcctttat
    22561 ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga tttaaaaaag
    22621 cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat aaaaccatta
    22681 atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg aacactcaaa
    22741 cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg taggaaacaa
    22801 ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac aattaatgca
    22861 catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag aatccaaatg
    22921 gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa tggaaagtca
    22981 tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta gaaaaagagc
    23041 atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat caatttttag
    23101 ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt gaaaaatatt
    23161 ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg gaaataaatg
    23221 aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct ctcacggaat
    23281 gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact ctttttcaat
    23341 gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat caaaagaaag
    23401 tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgatta gtgttgtggt
    23461 tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta gagcttttgt
    23521 agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt taaatctgac
    23581 tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa acgatatttt
    23641 acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt cggttgggaa
    23701 agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt caaaagctat
    23761 ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat ggtcgttcat
    23821 ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa gagatcaagc
    23881 aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa atgggctata
    23941 acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa gaagaaatcg
    24001 aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa tatcaacgga
    24061 agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa aatatcctcc
    24121 ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa aatccaacca
    24181 tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac gggggatttg
    24241 aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt cgtgaattac
    24301 ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat ttctatcgag
    24361 tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg gagatcgtaa
    24421 cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca gaagcaggtt
    24481 attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag atccgaatgg
    24541 atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca aaattgtgcg
    24601 aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt tcgaaagaac
    24661 aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga acggcagaag
    24721 agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg aaaaagcata
    24781 cccattct
//
LOCUS       DACECB010000041        24747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECB010000041 DACECB010000000
VERSION     DACECB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.041"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(57..173)
                     /locus_tag="IWO04_001708"
     CDS             complement(57..173)
                     /locus_tag="IWO04_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0213319.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1332)
                     /locus_tag="IWO04_001709"
     CDS             complement(418..1332)
                     /locus_tag="IWO04_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0213320.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="IWO04_001710"
     CDS             complement(1347..2348)
                     /locus_tag="IWO04_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0213321.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="IWO04_001711"
     CDS             complement(2345..4528)
                     /locus_tag="IWO04_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0213322.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="IWO04_001712"
     CDS             complement(4531..6978)
                     /locus_tag="IWO04_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0213323.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="IWO04_001713"
     CDS             complement(6962..7354)
                     /locus_tag="IWO04_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0213324.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="IWO04_001714"
     CDS             complement(7441..7938)
                     /locus_tag="IWO04_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0213325.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="IWO04_001715"
     CDS             complement(8055..8279)
                     /locus_tag="IWO04_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213326.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IWO04_001716"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IWO04_001716"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0213327.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /locus_tag="IWO04_001717"
     CDS             complement(11077..12282)
                     /locus_tag="IWO04_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0213328.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="IWO04_001718"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="IWO04_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="IWO04_001719"
     CDS             complement(12461..13846)
                     /locus_tag="IWO04_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0213329.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="IWO04_001720"
     CDS             complement(13875..14261)
                     /locus_tag="IWO04_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0213330.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="IWO04_001721"
     CDS             complement(14277..14591)
                     /locus_tag="IWO04_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0213331.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IWO04_001722"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IWO04_001722"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0213332.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="IWO04_001723"
     CDS             15851..17140
                     /locus_tag="IWO04_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0213333.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /locus_tag="IWO04_001724"
     CDS             complement(17179..17784)
                     /locus_tag="IWO04_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0213334.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="IWO04_001725"
     CDS             complement(17980..18444)
                     /locus_tag="IWO04_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0213335.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="IWO04_001726"
     CDS             complement(18466..18786)
                     /locus_tag="IWO04_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0213336.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IWO04_001727"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IWO04_001727"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0213337.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="IWO04_001728"
     CDS             20208..20558
                     /locus_tag="IWO04_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0213338.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="IWO04_001729"
     CDS             20743..21696
                     /locus_tag="IWO04_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0213339.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="IWO04_001730"
     CDS             21709..23496
                     /locus_tag="IWO04_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213340.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="IWO04_001731"
     CDS             23483..24685
                     /locus_tag="IWO04_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213341.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8230 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggag
//
LOCUS       DACECB010000042        24438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECB010000042 DACECB010000000
VERSION     DACECB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.042"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(369..1487)
                     /locus_tag="IWO04_001732"
     CDS             complement(369..1487)
                     /locus_tag="IWO04_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213342.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="IWO04_001733"
     CDS             1958..3265
                     /locus_tag="IWO04_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0213343.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="IWO04_001734"
     CDS             3384..4583
                     /locus_tag="IWO04_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0213344.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="IWO04_001735"
     CDS             complement(4610..5878)
                     /locus_tag="IWO04_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0213345.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6041..6687)
                     /locus_tag="IWO04_001736"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="IWO04_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="IWO04_001737"
     CDS             complement(7095..7598)
                     /locus_tag="IWO04_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0213346.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="IWO04_001738"
     CDS             complement(7721..8485)
                     /locus_tag="IWO04_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0213347.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IWO04_001739"
     CDS             8592..8867
                     /locus_tag="IWO04_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0213348.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9583..10536
                     /gene="yidC"
                     /locus_tag="IWO04_001740"
     CDS             9583..10536
                     /gene="yidC"
                     /locus_tag="IWO04_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0213349.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10658..11867
                     /locus_tag="IWO04_001741"
                     /pseudo
     CDS             10658..11867
                     /locus_tag="IWO04_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12177..12398)
                     /locus_tag="IWO04_001742"
     CDS             complement(12177..12398)
                     /locus_tag="IWO04_001742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0213350.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12559..13080)
                     /gene="tnpA"
                     /locus_tag="IWO04_001743"
                     /pseudo
     CDS             complement(12559..13080)
                     /gene="tnpA"
                     /locus_tag="IWO04_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13171..13537)
                     /locus_tag="IWO04_001744"
                     /pseudo
     CDS             complement(13171..13537)
                     /locus_tag="IWO04_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13551..13836)
                     /locus_tag="IWO04_001745"
                     /pseudo
     CDS             complement(13551..13836)
                     /locus_tag="IWO04_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13842..14036)
                     /locus_tag="IWO04_001746"
     CDS             complement(13842..14036)
                     /locus_tag="IWO04_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213351.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14036..14512)
                     /locus_tag="IWO04_001747"
                     /pseudo
     CDS             complement(14036..14512)
                     /locus_tag="IWO04_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14980..15333)
                     /locus_tag="IWO04_001748"
     CDS             complement(14980..15333)
                     /locus_tag="IWO04_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213352.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15461..16000)
                     /locus_tag="IWO04_001749"
     CDS             complement(15461..16000)
                     /locus_tag="IWO04_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213353.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16030..16221
                     /locus_tag="IWO04_001750"
     CDS             16030..16221
                     /locus_tag="IWO04_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213354.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16263..17129)
                     /locus_tag="IWO04_001751"
     CDS             complement(16263..17129)
                     /locus_tag="IWO04_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0213355.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17153..18805)
                     /locus_tag="IWO04_001752"
     CDS             complement(17153..18805)
                     /locus_tag="IWO04_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0213356.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18810..19712)
                     /locus_tag="IWO04_001753"
     CDS             complement(18810..19712)
                     /locus_tag="IWO04_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ0213357.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19796..21109)
                     /locus_tag="IWO04_001754"
     CDS             complement(19796..21109)
                     /locus_tag="IWO04_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0213358.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21177..22121)
                     /locus_tag="IWO04_001755"
     CDS             complement(21177..22121)
                     /locus_tag="IWO04_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ0213359.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22158..23171)
                     /locus_tag="IWO04_001756"
     CDS             complement(22158..23171)
                     /locus_tag="IWO04_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213360.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="IWO04_001757"
     CDS             complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="IWO04_001757"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ0213361.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6950 a         5537 c         4652 g         7299 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cctggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa gtaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc cacgaatgtc
     7201 acaatatatc cttctttata cttaggcggt gcgatcgttg caccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtcccctcg tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttgtacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgattt gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacgatccc tgctgcgact ccgacattca atgattccgc atttcctttc attgggatat
     7801 atacgttctg atcagtcgaa gacaagattt cctgacttac tccctgacct tcgtttccca
     7861 tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct tcttcattta
     7921 attctgtacc gtacaccgta aaccctttgc ctttcattac agggatcact tctgccaaat
     7981 ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac gcatctgccg
     8101 tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa agccagcctc
     8161 ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac tgctaagatc ccttgcggtg
     8221 taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta gaaatctctt
     8281 tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt cgatcagatg gaacccttcc aaaagatatt
     8401 ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttactga ttggatctct ttcacattcc tcacctcaac tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
     8641 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
     8701 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
     8761 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtttttgaa
     8881 gcgctttgcc tcgagtatta aggagaaaca ttttccaaat agcttctcat attttgaaat
     8941 gtaatgactt aataccgaga ggcagctgat aaaacaccgt caataaggtt gtgaaaaagc
     9001 tggtctgcat caagcattaa ggactggttg tgaaaaatag ctctccatat ttttcaacag
     9061 gctggactta atgccgtaga tgcaagcttt tgaacatcgt cgataaggtt gcgaaaaagc
     9121 tgatctgcat caagcattaa ggactggttg tgaaaaagtc gcttagtttt gagtattaag
     9181 gaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa taccaagaaa
     9241 ctgacttttg aacatcgtcg ataaggttgt ttttgaggca ctttgcctca agtattaaag
     9301 acaggtagta aaaaagctga tctataactt gcaataaatt tgcttaaccc atattttcca
     9361 gacagaacaa aaaagttttt agttttttaa ataagaaaga aacacttgga atcactcttt
     9421 tctttctaag tatttctttc ttgatttttt gtcttttttc aaaatttttc gctacgaaaa
     9481 agaacgaata tttatatgaa atttctatga agttgattga attccatgcg cttttttagt
     9541 atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa atggaaaaac
     9601 tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg tgtgcggatg
     9661 gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt ccatcctatg
     9721 ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg ggcagttatt
     9781 gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca atcgaaaaaa
     9841 acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc agcacaacaa
     9901 aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga aatgcagcaa
     9961 gtatatcgcg aaaatggatt gagtatgacc ggcggaatcg gctgtctgcc tctattgatc
    10021 caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg tatccgtgaa
    10081 tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgtggc catcgcaggg
    10141 ggcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga acagaaaaag
    10201 acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg
    10261 ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc
    10321 atcacaaacg ttataatgaa accgcgtttg aaagcacaag tggcagaaga gttgaaacaa
    10381 aacccgccaa aacaagtcgt tactccaaga aaagacgtga cagaatcagc agcaaagcca
    10441 gctgacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaacaacaaa
    10501 ggccgcaacg caggaaggca gcagaagcgg aaataaaaat atatgacaaa aaactgtaga
    10561 caaataaaaa aatttctttg ttctacagtt tttttgttac aattaaagaa aacgttcgca
    10621 tttcgaggag gaatgtataa tgatgtccaa ataatcaatg tatagtcgaa tggaagaaga
    10681 caaaatttcg ctagatgcac ttgttcctaa aaatcattta gtccgtaaaa ttgacaaatt
    10741 attgtcattt gattttattt atctcattgt tgaatcaact tactcgacca ttggtcgccc
    10801 tagcgttgat cctgtcgtct tagtaaaatt agtattcatt caatatttat ttggaatttg
    10861 ttcgatgcgt cagaccatta aataagttga aacaaaccta gcttatcgtt ggtttcttgg
    10921 gcttagttta attgataaag ttcctcattt ttcaactttt ggtaaaaact atgcacggcg
    10981 tttcagagaa accgatttgt ttgaacagat atttatcatt attctcgaat aagcagttga
    11041 tgctagattt attcatgaag aaattttgta tatggattca actcatatta aagcgaatgc
    11101 gaacaaacgt atttttacaa gagatatggc acataaagaa gccattttat accaaaaaca
    11161 attagaagaa gaaatcaatg aggcaagaat tgccgaaggc aaacgtcctt ttggttcaat
    11221 cattcgtcag gaggtggtcg aaagaaaatt tagcatcgca gatccagaaa gtggttatta
    11281 tgtaaaaaca gaacgagaaa aacagtttgc ttattctgct cataccgttg gtgatgaaaa
    11341 tggttttgta ttagatgtca tgattacgcc tggaaatcta catgatagcc gaatgctcgt
    11401 gccacaaatc gaacgcgtta aatcacgtta tggtgtagct gctaatgctg cgtacaaaac
    11461 accttggaac gtcaagtatt taatcgaacg aaaactacga cccattctcc cttatatcag
    11521 tttcgcacaa ctactcgaga tggctataaa aaatatgtat ctaaatcttt tatttgtgaa
    11581 agttgtccat tattgaaaaa ttgtacagaa agccaaaaac accaaaaaga aatacatcga
    11641 catatttggg aagactactt agatgaagca gaacatttac gccatacggt gtacaatcgt
    11701 gaaaaatata aaaaaagaaa agaaacgatt gaacgcattt ttgcagacgc aaaggaaaaa
    11761 catggaatgc gctacaccaa gtatcgaggc ttggaaaaaa tcaggtgcat acaatgcttg
    11821 tttttgtcgc aatgaatctg aaaaaattag ctacttggca gtgggagcat atgtcgttat
    11881 atcttttatt atacaaaaat agacaaaagt atcagtctac actcttagta aagaggaaac
    11941 tgatgctttt gtctacagtc ttagagagtg ggacaatagt tttttcttct ttacgtcttt
    12001 tcctctccat gtattttcct aattttctta atttaacgcc atgagtgcca agccaatgtc
    12061 aatcttggct ccttgtttcc ttcataagtg gaaatccgtt tccgatagat cgaaccttct
    12121 ttttcctcta aaagtttttt tacattctgc tttgaaataa cgccatgtat tattcactaa
    12181 aatttgtcgg tttcgctcac tttttgcttt cgtacattga ctacgaacag gtcagcaccg
    12241 accactttca cattcgtaaa ttttgaagac acgtataaat ccacttttat cttttcttcg
    12301 actaaagcgt tgaccgcaaa agcgacatct gtttcagaaa tatattgttc catatctaat
    12361 ggtaaaattt tttggttgat ggtataattt ttaaacataa agcactcgct tcctttctga
    12421 ttgggtttgg tcactttaat cctacaggga tgagtgcttt ttgtgtacct taaaatcagt
    12481 aaatatccat ccgtgaaaac gggtggcttt ttaacagccc tagaagggct caccactcac
    12541 agccctatct ctctttaact atttaaatgg atctacatac tcttgtttac ttatactgtc
    12601 tctaattcga tcatctgctt cttgttctca aatatatatt tcaattgttt tttgatttaa
    12661 cccaaccgta ctcacgtagt atcctttggt ccaaaagctt ttattcccat acttatattt
    12721 caaatttgca tgtttttcat ggattaaggt tgcacttcgt ccttttaagt agcccataaa
    12781 actagaaata ctaatctttg gcggtattct tactaacata tgaatgtgat caggcattgc
    12841 atgagcttca atgatttcaa catctttcct ctcacacaat tttcttaata tctggccaat
    12901 acttactctt aactttccat aaatcacttt tcgtcgatac ttcggggtaa agactatgtg
    12961 atacttacaa tcccatctcg tatgtgctaa acttttatcg ctaaagctat ttcgtccacg
    13021 cgtagaaacg cgcgtttttt tgtttcgtac gctacgccaa cgaaactgac taaagtcaat
    13081 aaaaaaagtg gggcaataat cagatttttt tctgactatt gtcccactct ctttttatta
    13141 ctcttcatct tctactattc cactaattaa ttagttgaca gatacaatgc tttcacatcc
    13201 tgcacgaaca aactcgggct ctgaaccaat cacttgcgag ttgataaatt caatgaccat
    13261 aggaagatta atccgacata aagtttaatc tgttgtcctt ccattatcag tttagaaatg
    13321 atattagtag gtgtaccgcc taaaagatcg catagtacca caaaatcatt taacgaatct
    13381 atgacagtaa gagcttttgt ctgtatgttt cagtgccatc ttctggtaat aaaggtaacg
    13441 tgtagatatt ctcttgaggt cccataatca tttttgtact ttttttcaat tcctcacaaa
    13501 attttccgtg actaatcaat atcaattttc tactcatttt tggctctccc ttatttcgaa
    13561 atcacacaca atgcagataa agccacacac aagtaatacg ataaaaatcg ctcgagttgg
    13621 gtcatatttt tctttcctaa caaccaataa acacctcgac gatcccagcg agtaagattt
    13681 tagataatat catatcgata tagttttgca tattcaatgt cacatcgcca atcgttggta
    13741 ccataaaaat ttaatatcaa tcattgttgc aactaatgct cctaccatga acactcttaa
    13801 taaagtcgct acgtccgtta atacagttaa gcgagattac actagatgat ctgttcctgc
    13861 caaggcaaga gccagaagcg gttgcctgcc catttgcctt tggtgtgtcg gagctgtccg
    13921 ataaagttca ccgcccgatc cgctctggct ttgtcgtagt gacattccga aagcatgaat
    13981 cggcttggtg tgtattcctt taatttggga tagccctttg gtctggtttc tgccatcagc
    14041 taccacctcc caacagagcc tccatcgcat cttctgctcc gacaccaccg ccatcaccgg
    14101 caatgatacg gcttctggac gaaggcgtca gaccgaactg ctcggaaaat cggttcataa
    14161 ttttcagata ggtctgggca ataaacacct gtggtacctg ctgccaatag ccggataggg
    14221 ttttcacgat gataccgtgc tgggtaatga attcctcggc ttccttccag cgggcgtagg
    14281 cttggcacta accggcaaag gcagccatat ccacatctgt caagattccg atggcctcca
    14341 tctgctttgc cagacgcttc cattccttct ttgcctctgg ttccagccac ttcggacagg
    14401 tcggtgcctt cttttccggc gttgactctt ttgcattgag tgggcgattg cccggatttc
    14461 cctccagctt cttcatggct gtcggggttg attttcttcc tctggttgcc acagtcgtca
    14521 cctcccttca atgtatttct gccagtagac agagaacaga gccaagtcga aacctgactc
    14581 tcttctctat ctgctaactt gtatttcagt ccactcttct gatgaatcgc acccgcagga
    14641 ttttcaagat gccgcaggca aattccaaga agtttctgac cgcatcataa ccatcgtagc
    14701 cgtgtagcca catccttcca cctcgccgcc gtcctcgtcg ctgatataaa tgattctgta
    14761 ttcgctcatc tgttgttttc tccagtcttt tgtttttttg tagtacatat atcactctaa
    14821 aagtgattat tatcaagtcg tttgaaagcc ataatgtaca caatcattcg actggttttt
    14881 ggttcataat tgaccgccaa agtgagcaaa agaaagggac ctctgtctgt ttcagaagtc
    14941 cctttcggta tttggttctt agtggttcag gctgtattct caaacctcat actcatcttc
    15001 gaggtctgcc tctgcaatga tctggccgat gctgtcgtaa cgctggacct gcatctggat
    15061 ggcatccttg tctgctccct cgtccatcat cttcagcagc ttgtctgatt cctttttgta
    15121 ggcccagaaa aggatctcct gaaggctggt ctcctgcttt ttcttgcgaa gggcgacctt
    15181 cttctcttcc accgcctcgt tcaacttggt gaactctgcc caagcaagga taccgtctcg
    15241 cttgattgcg atctccacat ccttcagctc cgggatggtc tttgcaccct tgatctcctt
    15301 caaatatttc tttgcctttt cgctcattgc cattagcgtt tcctccttgt ttttgggatt
    15361 ttcccctttg gttgtgtgta tattgccata gtctctcgac tatatcaagt caatccacag
    15421 gcatatacta cacaatcatt tggagctgta tttgtataca ttatgccgat ttatgagaga
    15481 agaaaaggct ccagccgttc gactggaacc ttgggggggt cggtttctta ggcggcgtgg
    15541 cggtgtagac cttgaggtag ctgatgttct tccagacgtc cgtgcccttc agtctgacca
    15601 tgccgtccag ctctcggtcg agggtgaact tgcggaaaag cttgatgcgg ttgaggctgt
    15661 ttgctagctc cttgccgttc ttggcatcca gaactgcgtc ctcgatctcc gcaatactaa
    15721 tttcctgtct aaaccaaagg ttgctcaagg cttcgggaat gccgtttgcg gtctgcaggg
    15781 ggttgaaagt ttccaataca gcaggggtcg aaaccacctg tccgagctgg aattttgcgt
    15841 tccctaaaat catctgcata atttcttcca tgtgaatcat ccatttctgc cgttgtggta
    15901 ttccctttgg cagtacagta ctatatatca ctctgaatcg tacatttatc aagaagcgga
    15961 tttttgtgga atcaccaatt cgatataata gtaattgcat gaaaaatcta agcactggat
    16021 tggtaggtga tgattatacg tgacgatcac atttacacct gtgattcctg ccactacagc
    16081 ttccctgccg acgaacagcc agagcgttgt cctgactgcg aaaagacagc aactcggctg
    16141 gataccgaaa tcgaaactga agattactat cgtgttcgtg ctgaaatcaa agctgagatt
    16201 aaagctttga atgcgggtta acgacaggta ctgaccggcg gctcaacacc gccggctttt
    16261 tattaccaca tcttgcggaa ttcgctggga cgaacacctt ccaccttgaa aaaagttctg
    16321 gaaaaatagt ttgcatctgt tatgccaatt tttgttgcaa tctcttccat acttagattt
    16381 gtaaagcgta gcaagtgctt ggcctgtgtg attcgtttct gattcagata accgacaaca
    16441 gttgtaccgt aagtttcacg aaaaatctta gctaagtact gtttattgat aaaaaactct
    16501 gcactcaact gatccagcgt aatcttctcc gtgtaatggt catccagata ttgttttatg
    16561 ttctgcaatt ccattttttt tgtagagaaa ttcggcactt tgtcaggatt ccaagaatct
    16621 tccatcaaat gcatcagcag ttcactgagt agaacattga ttttcatatc ccggacatac
    16681 tcctgtccct ctgccaccat atgaaggctc ctcagcaagt cagcatactt atctgttttt
    16741 gatgttttaa agaccggttg accacctcgt tcaaggtatt ttttgtagac ttccgtcata
    16801 ccaatgccat taaaatggca ccaccgcaaa gtccacaaat catcttctga tgactcatgg
    16861 acatacggct tcaaacagtc aataaacacc atatctccta cttgaagcgt atatttctgc
    16921 cccttatact ccaatgtgcc tgaaccaact tctaccagga aacaaaggta agatgacaat
    16981 cctgtacgcc tagatgtatg tgccttcaga gcagtcagct gtccaatttc ctgcaaatac
    17041 aaaagagagg atcttgcaaa gaacgatgcc gtgtacagca cacgatttga cttcaccgat
    17101 ttcaaagcgt tagaaaacag ttcctccatt acaagatttc tcctttcttt tcttactcga
    17161 acatttccgc cagttcagca gccataatag cttccatagc ttcggcggct tccttgttct
    17221 ctgcgctgac gctgatatag gtcttcagct tcggctcagt accagaagga cgaaccacaa
    17281 cagagcagtt atcttccagc ataaacttca aaacatcaga cttcggcaga ccattcaggc
    17341 ccttactgta gtctaaagtt tcaataacct tcttaccacc aattatatcg agacccttgt
    17401 ggaattcaac cataatttcc tgcatcttgg taaaacctgc cgcaccgtca aactcgaagg
    17461 aatgcagggt gttcaaacaa tagccatact ttgtataaat ctcgttcagc ttatccagca
    17521 ggctgatacc ctgagtcttg tagtaagaga acatttcaca gattagataa gcagcgttaa
    17581 caccgtcctt gtcacggaca tagctaccgg acagatagcc gtaagattcc tcaaagccga
    17641 agatatagct gtcaacctta ccagcctttt ccaacatacc aatctgctca ccgataaact
    17701 taaagccggt cagcacatcg atggtacgaa caccatagtt ggatgcgata cgttcaccca
    17761 aatccatggt gacgatggtc ttgaccatca cagggtcagc aggcattttt ccatgcttaa
    17821 ttctctgaga acagatgtag tccagaagta gcagaccagt ctcattacca gacaacagaa
    17881 catagtcacc atgtgcgctc ttaactgcga taccaacacg gtcgcagtca gggtcagtgg
    17941 ccaacagcag atccgcattg tacttcttgg catactccat acccagagac atagcttcct
    18001 taatttcagg gttcggataa ggacaagttg ggaaattacc gtccggcaga cgctgttctt
    18061 ccacaacggt cacattgctg tatcccatct ctgccagggt gcgggtaaca ggcaccagac
    18121 cggtaccatt cagaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca
    18181 ggacggactg ccccttcact tcttcgatga aagcagtcag aacttccgac tgaatggatg
    18241 caatcttaga agcatcgcca tccatcttta catcagcaaa gatgtccagc ttttcgattt
    18301 ctgccagaat tgcagtagca gcctcggtag taatctgaca gccgtctgca ccgtatacct
    18361 tgtagccgtt atatttagaa gggttatgag atgccgtaat cataacaccg gcatctgcct
    18421 tcagataacg agtcgcaaaa gatactgtag gaacaggaag cagttctggc cacaagtgca
    18481 cctgaatacc atttgcagca aagatgtctg cggcagtctt tgcaaacaca tcagatttga
    18541 tacgggaatc atagccaacc accacggaag cggcttcata gttcttattc agatagtctg
    18601 ccagaccttg gcttgcctta cctacggtgt gtacgttcat gcgattagtg cctgcaccga
    18661 tggtaccacg cagaccacca gtaccgaaag acaaattttt atagaaagag tcctcaatgt
    18721 ccttctcaga ataggaacgc agttcttcta taatatctgc attggcatga ttcagccaac
    18781 gctcatattc tactttataa ttcatattat tatcctctca catccagaaa ctgaatcgac
    18841 ccgtgaatgc tgatttcagt agaatcggcc ggcacaaaaa ggcagtcccc cttatagatg
    18901 tccaacgatt cattatcaaa tcgaacggta ccacagccat tgacacacag caacacacgg
    18961 aagctgattg aatccgcacg atagtgtacc gtctgtcttc tctcagtgtt gacaatcata
    19021 cgagagacct cgaaatgctt gcatctgcaa agcaattccg cagctacacc agcacgatat
    19081 tttagaacac gaagcggctg tttcggttct gacaaccctt tcaagttcgc tacttccagc
    19141 gctttgtcta catgaagttc tctcttttta ccatttttgt caacacggtc ataatcgtag
    19201 agacgataag tcagattgga attttcctga atttctgcaa ttagtgcacc cgcaccaatc
    19261 gcatgaatcg ttcccgcctc aataaagaac acatcattct ttttgaccgg aaccttctga
    19321 agataacgct caacagttcc ctgagcaatc gcttcacgga tcacagcttc ttctgttatt
    19381 ttctgcagcc catagattaa gctggcatcc agtacatacc acatctctgt ctttccaagc
    19441 tgcccgtttt ctttttcaat tgcatattca tctgtgggat gtacctgaac agacaaatcc
    19501 tttttcgcat caatgaattt gatcaggatc ggcaattctc ctttggtctg cggatgcgtt
    19561 cccagaaact ctgggtgttc ttgcaacacc tccgtcaagg tcatcccctg atactctgac
    19621 cagacacaac aaagctcggt ccatccgggt gcgtagagca ttccaagttt ccgcaagagg
    19681 agacatctca attccttttg caaattcatc attcaggcgg ttccccccca cagataatcc
    19741 tttgcggaag gcttcaataa aaacggtttc tgtataacga gccctcctcc gttttttaat
    19801 actctaactc aaatttctgt ttatctttaa cattgatatc cttgccaagc tgtacttcaa
    19861 tcagcttcag ctcagtatct gccataatgg tatgccgaca gccagcactc atggtgatca
    19921 catcgccagc cttaacaacc tgctcatatc catccacgat ggtcttacca gtaccggcca
    19981 gaaccaccca cacctcgtca cggttcttgt ggctgtgata attcatggag tgactagcat
    20041 tcagagtaac cttaatggtc atgctctctg cttctacatc cataacctta taactacccc
    20101 aagacttctc tgcgaacatg atctgctgct cgaacttgtc aacgaaaggc ttaatatagc
    20161 tggactgttc cttatcggaa accagaatac cttcccgaga tgcacagatg accacatcat
    20221 gcagacccat agccaacaca ggaacatcca gttcgttcag gatgtgtacg ccatcgcaat
    20281 tctcattcat aacaccttta ccgataatgg attcctccat accttcggtc agagtattcc
    20341 aagtccccag atccttccac ataccagcaa aacgctgtac ctgaatcttg tcctcatgct
    20401 caacaacggc atagtcgaag gaaatcttag tcagagtatc gtatttgttg aacagatcct
    20461 gataatctac aaagtcaatc agctgatgtg cacgctccat gacatacttc agcttgtaga
    20521 caaacacacc accgttccac agtgcaccct gtgcgatata ttcctttgcc acagcctcag
    20581 aaggtttttc cttgaaggta tttacgaaag cagtctgctc tgcgctctca ggaatgatgt
    20641 agccgtactt ctcggaagga taagtcggtt caatacccat gagcaccaga ttagcttcgc
    20701 ctttgtcagc ctgatcggac agagatttca gggcctcaaa atagtcatct tctacgtaag
    20761 ggtcaaccgg gcaaacaact acggactctt ctggatcaat gcccatcaca tctaccagat
    20821 aagcggtagc caatgcgatt gccgggaagg tatcgcggcg acaaggctct acagatacac
    20881 ccacatcctc accaagttgg ttatggatag cagaaacctg tgtcttagag gttgcaatcg
    20941 taacagttgc gtcgttatct accttcttaa tctggcggta gacacgctga accatggatt
    21001 catatccatc ttcagttttg aaaatcttaa tgaactgctt ggaacggatg tcattagaca
    21061 gcggccaaag gcgcttacca gaaccgccag acagtaaaac aatattcata atgatctctc
    21121 ctttttataa gaaagaggct gctgcaatcc tcaaaaatca cagcaacctc ggtaaattag
    21181 cgttcagcac gcatcgggtt attcaggaag tcctcgtagg acagacggat gccatcttcc
    21241 aactcagtct tatatgtcca tcccagcttg gttgccttgg aaacatccag cagcttacga
    21301 ggtgtaccgt tcggcttgct ggtatcccac agaatctcgc cttcgtaacc aataaccttt
    21361 gctaccatct cggtcagttc cttgatagac agttccttgc cagtacctgc attaacagtc
    21421 tcgtcaccgc tatagttatt catcaggaac acacacaggt ttgccagatc gtcaacatat
    21481 aggaattcgc gcagaggaga accatcaccc cagcaggtaa cagtaggcag acctgcttcc
    21541 ttagcctcat ggaagcgacg aatcaaagcg gggagcacat gactgtgagt aggatgatag
    21601 ttgtcgttag gtccatacag gttagtaggc ataacagaga tatagtcagt accgtactgc
    21661 ttgtttagaa actcacagta cttcaggcca gaaatctttg ccagtgcata agcctcgttg
    21721 gtcttttcca gctcacttgt cagcagacag ctttccggca tcggctgcgg tgccatacgc
    21781 ggatagatac aggaagaacc gaggaactgt aacttcttac agccattctc ccatgcggag
    21841 ttgatgacgt tcatttccag aatcatgttt tcatacatga agtctgccag agcgttctgg
    21901 tttgcgataa taccgccaac ctttgcagca gcgagaaata cataatccgg cttttcctgt
    21961 gcgaaaaatg cttcaacagc gtcctgacgg cacaggtcaa gctccttatg ggtgcgggtg
    22021 ataatattgt tgtagtcctg acggttcagc tcgcgcacga ttgcggaacc agccatgcca
    22081 cgatgaccgg ctacgtagat ttttgcgtct ttttccatca taataatgct tgctcctttc
    22141 taaatttaga gcatctctca ttgcatgaga gaaatcaatt tgtctgcgat ttcgctcgca
    22201 gcatcataaa atgtatcaaa atttgaggtg tctagctgtt tgaggtcctc aatcacccgc
    22261 tctacgcggt ggaggttttc cggtgtgacg gaaattgcca gacccagttt ttgcaattta
    22321 caaatagttg ttctatcttc caaaactgta tcgcgggcga acagtgccat cggacgctgt
    22381 gcgagaatac actccgcaac agttccccat ccagcctttg taataacgta atctgccgcc
    22441 gctatatagt cctgtgtatt gggggtgctg ttagaaataa cggttacgtt ttctcccaca
    22501 agcggaacac ctagcgttgt aatgaaatca tacggcgcgt gactgacatc aatcggctgt
    22561 ttgaaccgcg cggacatacc gagtgcgaca aatacaggcg gtttttcatg ctgtgattta
    22621 atgcgttgca cttcgtcgtt atgaatcgga cgcgccacaa cggaagtcgg tgttttttcg
    22681 ctgttttgga gaaattccaa catttcttcg ttatttagcg cataaagcat ggtgtgctga
    22741 attttaccgt aacgcttggc atattctttc caaatatgtt cgggtaaaaa ttcgcgatac
    22801 agctctgtcc aagtgaagtt tcctacatac aggaaaggta ctcctgccat atcgcaggca
    22861 tccattgccc agattggcat atcgcaaagc actgcgctga cagcttctcg cttgagccat
    22921 tcggcttctg tctttgccaa agaaggaacc tttgccaaaa aatattcaca gatctcggtc
    22981 agcttacccg tgtccaccag tagggtgccc tcacggttaa tcagaccgat atctgtgttt
    23041 attggacgat agcgcacgcg gtctatcagc tctttgggca gttcctgctc caaattgcgc
    23101 tttgcaaaat ccagatgatt tgcgccgcaa accagtgtaa tcttcgcttc gcatcggcag
    23161 agtgttgcca taataatggg cagattccgc gcgatatgtc cgaagccgtg actggaaaga
    23221 tagaaacaga tatgtttatt caatgtcagc ttacttgact gcgttcttca ttgcagcttc
    23281 gcgctttgcc aattcacggt catgctttgc catgatgtta accagttcct cgtaagaggt
    23341 cttctgcgga ttccaaccga gaacggtctt tgccttagtc gggtcgcccc acaggttgtc
    23401 aacgtcagtc ggacggaacc actgtggatt tacacaaacg agcatcttgc cggtttcaac
    23461 atcgtagccc ttttcatcaa tgccagtgcc ttcccaacga atggtcatgc catttgctgc
    23521 gaatgccttc tcggtgaagt cacgaacagt gtgctgctcg ccggtagcga tcacgaagtc
    23581 ctcgggagtc tcatgctgga gaatcatcca catgcactca acatagtcct ttgcatagcc
    23641 ccagtcacgc agggagtcca tgttgcccag ttccagatgg tcctgcaggc cttctgcaat
    23701 gcggccggca gcgagggtga tcttacgggt gacgaaattc tcaccgcgac gttcggattc
    23761 atggttgaac agaataccgt tgacagcgaa catgccataa gcttcacgat attccttagt
    23821 gatccagaag ccatactgct ttgcaacagc ataagggctg taaggatgaa acggagtggt
    23881 ttcacgctga ggaacttctt caaccttgcc atacagctca gaagtagatg cctgataaat
    23941 cttagtcttc ttcgtcaggc ccagaatacg gcaagcttcc agaatacgca taacgcccaa
    24001 agcatcaaca tcgccggagt actcaggaac atcaaaagaa acctgaacat ggctctgtgc
    24061 agcgaggtta tagatttcat caggctgaac aatgctgatg atacgaatca gagaagatga
    24121 gtcggacagg tcaccgtcat gaagggtgat cttgcccatc aaatggtcaa tgcgagcggt
    24181 attggaaata gaagcacggc gagtgatacc gtgaacttcg tagcccttct ctagcaggaa
    24241 atctgccaga taggaaccgt cctgaccagt aataccagta atcagcgctt ttttcatgat
    24301 acattatcct ttcctatgta agtgataagg ccatataaat ggctcgtaat ttctaatact
    24361 tttcaagaat acacgataat tttcgatatt tcatagaaca atctacgcac gcattagcac
    24421 aatattagtt tttcgtga
//
LOCUS       DACECB010000043        24395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECB010000043 DACECB010000000
VERSION     DACECB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.043"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            115..696
                     /locus_tag="IWO04_001758"
                     /pseudo
     CDS             115..696
                     /locus_tag="IWO04_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            700..1341
                     /locus_tag="IWO04_001759"
     CDS             700..1341
                     /locus_tag="IWO04_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213362.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1338..2255
                     /locus_tag="IWO04_001760"
     CDS             1338..2255
                     /locus_tag="IWO04_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0213363.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2252..2923
                     /locus_tag="IWO04_001761"
     CDS             2252..2923
                     /locus_tag="IWO04_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213364.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3464..3952)
                     /locus_tag="IWO04_001762"
     CDS             complement(3464..3952)
                     /locus_tag="IWO04_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0213365.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4153..4419)
                     /locus_tag="IWO04_001763"
     CDS             complement(4153..4419)
                     /locus_tag="IWO04_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0213366.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4419..4682)
                     /locus_tag="IWO04_001764"
     CDS             complement(4419..4682)
                     /locus_tag="IWO04_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0213367.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4675..4947)
                     /locus_tag="IWO04_001765"
     CDS             complement(4675..4947)
                     /locus_tag="IWO04_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213368.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5158..5343
                     /locus_tag="IWO04_001766"
     CDS             5158..5343
                     /locus_tag="IWO04_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0213369.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5485..7308)
                     /locus_tag="IWO04_001767"
     CDS             complement(5485..7308)
                     /locus_tag="IWO04_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0213370.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7906..10611)
                     /locus_tag="IWO04_001768"
     CDS             complement(7906..10611)
                     /locus_tag="IWO04_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0213371.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10917..11864)
                     /locus_tag="IWO04_001769"
     CDS             complement(10917..11864)
                     /locus_tag="IWO04_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0213372.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            12054..12323
                     /gene="rpsN"
                     /locus_tag="IWO04_001770"
     CDS             12054..12323
                     /gene="rpsN"
                     /locus_tag="IWO04_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0213373.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12499..12861)
                     /locus_tag="IWO04_001771"
     CDS             complement(12499..12861)
                     /locus_tag="IWO04_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0213374.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12872..13993)
                     /locus_tag="IWO04_001772"
     CDS             complement(12872..13993)
                     /locus_tag="IWO04_001772"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0213375.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(14009..14359)
                     /locus_tag="IWO04_001773"
     CDS             complement(14009..14359)
                     /locus_tag="IWO04_001773"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0213376.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14489..16000)
                     /locus_tag="IWO04_001774"
     CDS             complement(14489..16000)
                     /locus_tag="IWO04_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0213377.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16322..17674)
                     /locus_tag="IWO04_001775"
     CDS             complement(16322..17674)
                     /locus_tag="IWO04_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0213378.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17827..18903)
                     /locus_tag="IWO04_001776"
     CDS             complement(17827..18903)
                     /locus_tag="IWO04_001776"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0213379.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            19099..20844
                     /locus_tag="IWO04_001777"
     CDS             19099..20844
                     /locus_tag="IWO04_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0213380.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21124..21561)
                     /locus_tag="IWO04_001778"
     CDS             complement(21124..21561)
                     /locus_tag="IWO04_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0213381.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21545..22918)
                     /locus_tag="IWO04_001779"
     CDS             complement(21545..22918)
                     /locus_tag="IWO04_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213382.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23240..23728)
                     /locus_tag="IWO04_001780"
     CDS             complement(23240..23728)
                     /locus_tag="IWO04_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0213383.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23750..24352)
                     /locus_tag="IWO04_001781"
     CDS             complement(23750..24352)
                     /locus_tag="IWO04_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0213384.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6998 a         5196 c         4510 g         7691 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
      181 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
      241 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
      301 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
      361 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
      421 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
      481 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
      541 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
      601 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
      661 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
      721 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
      781 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
      841 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
      901 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
      961 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
     1021 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
     1081 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
     1141 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
     1201 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
     1261 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
     1321 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
     1381 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
     1441 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
     1501 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
     1561 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
     1621 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
     1681 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat ggttttgaaa
     1741 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
     1801 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
     1861 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
     1921 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
     1981 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
     2041 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
     2101 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
     2161 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
     2221 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
     2281 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
     2341 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
     2401 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
     2461 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
     2521 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
     2581 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
     2641 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
     2701 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
     2761 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
     2821 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
     2881 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
     2941 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
     3001 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
     3061 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
     3121 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
     3181 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
     3241 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
     3301 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
     3361 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
     3421 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
     3481 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
     3541 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
     3601 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
     3661 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
     3721 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
     3781 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
     3841 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
     3901 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
     3961 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
     4021 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
     4081 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
     4141 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
     4201 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
     4261 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
     4321 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
     4381 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
     4441 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
     4501 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
     4561 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
     4621 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
     4681 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
     4741 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
     4801 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
     4861 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
     4921 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
     4981 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
     5041 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
     5101 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
     5161 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
     5221 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
     5281 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
     5341 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
     5401 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
     5461 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
     5521 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
     5581 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
     5641 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
     5701 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
     5761 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
     5821 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
     5881 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
     5941 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
     6001 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
     6061 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
     6121 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
     6181 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
     6241 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
     6301 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
     6361 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
     6421 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
     6481 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
     6541 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
     6601 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
     6661 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
     6721 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
     6781 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
     6841 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
     6901 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
     6961 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
     7021 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
     7081 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
     7141 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
     7201 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
     7261 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
     7321 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
     7381 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
     7441 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
     7501 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
     7561 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
     7621 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
     7681 catctgcggt aataagctca accaaattaa aaacataaga agctgttttt atttggtcgt
     7741 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
     7801 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
     7861 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
     7921 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
     7981 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
     8041 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
     8101 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
     8161 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
     8221 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
     8281 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
     8341 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
     8401 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
     8461 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
     8521 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
     8581 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
     8641 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
     8701 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
     8761 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
     8821 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
     8881 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
     8941 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
     9001 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
     9061 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
     9121 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
     9181 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
     9241 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
     9301 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
     9361 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
     9421 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
     9481 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
     9541 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
     9601 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
     9661 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
     9721 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
     9781 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
     9841 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
     9901 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
     9961 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    10021 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    10081 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    10141 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    10201 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    10261 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    10321 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    10381 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    10441 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    10501 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    10561 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    10621 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    10681 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    10741 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    10801 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    10861 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    10921 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    10981 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    11041 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    11101 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    11161 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    11221 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    11281 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    11341 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    11401 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    11461 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    11521 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    11581 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    11641 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    11701 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    11761 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    11821 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    11881 tttctttaag tttacgttta tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    11941 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    12001 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    12061 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    12121 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcataaactt cctttagatg
    12181 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    12241 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    12301 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    12361 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    12421 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    12481 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    12541 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    12601 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    12661 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    12721 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    12781 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    12841 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
    12901 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
    12961 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
    13021 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
    13081 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
    13141 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
    13201 cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct
    13261 gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc
    13321 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
    13381 catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt
    13441 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
    13501 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
    13561 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
    13621 ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag
    13681 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
    13741 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
    13801 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
    13861 cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
    13921 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
    13981 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
    14041 tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa
    14101 caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
    14161 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
    14221 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
    14281 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
    14341 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
    14401 accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc
    14461 ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc
    14521 gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac
    14581 cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc
    14641 caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg
    14701 tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa
    14761 ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta
    14821 gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga
    14881 atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa
    14941 tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc
    15001 cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat
    15061 agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta
    15121 caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag
    15181 acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt
    15241 cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa
    15301 acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga
    15361 tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt
    15421 taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt
    15481 gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt
    15541 catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa
    15601 tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac
    15661 ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc
    15721 gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa
    15781 ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg
    15841 cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta
    15901 gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt
    15961 tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt
    16021 gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt
    16081 ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga
    16141 aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc
    16201 attattatga catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct
    16261 tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt
    16321 tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt
    16381 ttagcataat actatctgtt gctgcaagat tttgtttgat agcatcaatc aatttattct
    16441 tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta
    16501 gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt
    16561 cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca
    16621 cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg
    16681 ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc
    16741 attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg
    16801 acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc
    16861 caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt
    16921 ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta
    16981 ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt
    17041 ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa
    17101 gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa
    17161 gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg
    17221 tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt
    17281 gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg
    17341 ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg
    17401 ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg
    17461 gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct
    17521 ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta
    17581 tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca
    17641 cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg
    17701 aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc
    17761 tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat
    17821 ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa
    17881 ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg
    17941 gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt
    18001 tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc
    18061 taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg
    18121 catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac
    18181 gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc
    18241 gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa
    18301 agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt
    18361 cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt
    18421 aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc
    18481 agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc
    18541 tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc
    18601 gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt
    18661 gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa
    18721 gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt
    18781 tgtaataaat acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa
    18841 tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt
    18901 caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca
    18961 aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag
    19021 cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa
    19081 agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt
    19141 cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag
    19201 gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca gatgctttct
    19261 gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt
    19321 gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca
    19381 ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag
    19441 gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac
    19501 atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa
    19561 tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc
    19621 ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag
    19681 aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa
    19741 gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac
    19801 aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag
    19861 cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct
    19921 catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga
    19981 aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct
    20041 gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat
    20101 ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat
    20161 gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa
    20221 agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat
    20281 gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg
    20341 cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc
    20401 cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat
    20461 tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa
    20521 gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact
    20581 tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc
    20641 gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc
    20701 tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata
    20761 tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa
    20821 atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa
    20881 taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc
    20941 gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag
    21001 taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt
    21061 ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa
    21121 accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa
    21181 gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg
    21241 ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg
    21301 cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt
    21361 cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac
    21421 gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac
    21481 cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc
    21541 tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta
    21601 atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt
    21661 ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt
    21721 cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat
    21781 tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat
    21841 aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac
    21901 ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt
    21961 gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg
    22021 caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca
    22081 gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa
    22141 aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa
    22201 acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat
    22261 cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa
    22321 caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg
    22381 gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg
    22441 ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag
    22501 ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat
    22561 tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa
    22621 aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa
    22681 tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta
    22741 aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat
    22801 tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta
    22861 agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga
    22921 caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga
    22981 aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct
    23041 tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt
    23101 cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct
    23161 ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac
    23221 tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa
    23281 atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa
    23341 cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt
    23401 gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt
    23461 tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt
    23521 aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt
    23581 tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa
    23641 tgatcggcac aaaagcttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt
    23701 cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac
    23761 cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc gaccatggtt
    23821 tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta
    23881 gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa
    23941 tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa
    24001 taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa
    24061 tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg
    24121 ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa
    24181 caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg
    24241 atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa
    24301 taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc
    24361 cccacatctt ttttcattta ttaaaactgc ctcct
//
LOCUS       DACECB010000044        24209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECB010000044 DACECB010000000
VERSION     DACECB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.044"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(30..575)
                     /locus_tag="IWO04_001782"
     CDS             complement(30..575)
                     /locus_tag="IWO04_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0213385.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(587..1279)
                     /locus_tag="IWO04_001783"
     CDS             complement(587..1279)
                     /locus_tag="IWO04_001783"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0213386.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1298..1582)
                     /locus_tag="IWO04_001784"
     CDS             complement(1298..1582)
                     /locus_tag="IWO04_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213387.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1595..2158)
                     /locus_tag="IWO04_001785"
     CDS             complement(1595..2158)
                     /locus_tag="IWO04_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0213388.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2386..3048
                     /locus_tag="IWO04_001786"
     CDS             2386..3048
                     /locus_tag="IWO04_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0213389.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3172..4368
                     /locus_tag="IWO04_001787"
     CDS             3172..4368
                     /locus_tag="IWO04_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0213390.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4469..5845)
                     /locus_tag="IWO04_001788"
     CDS             complement(4469..5845)
                     /locus_tag="IWO04_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0213391.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5882..6748)
                     /locus_tag="IWO04_001789"
     CDS             complement(5882..6748)
                     /locus_tag="IWO04_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0213392.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6837..7862)
                     /locus_tag="IWO04_001790"
     CDS             complement(6837..7862)
                     /locus_tag="IWO04_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0213393.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7880..8554)
                     /locus_tag="IWO04_001791"
     CDS             complement(7880..8554)
                     /locus_tag="IWO04_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213394.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8738..9631)
                     /locus_tag="IWO04_001792"
     CDS             complement(8738..9631)
                     /locus_tag="IWO04_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0213395.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9705..11402)
                     /locus_tag="IWO04_001793"
     CDS             complement(9705..11402)
                     /locus_tag="IWO04_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0213396.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11395..12708)
                     /locus_tag="IWO04_001794"
     CDS             complement(11395..12708)
                     /locus_tag="IWO04_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0213397.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12708..16226)
                     /locus_tag="IWO04_001795"
     CDS             complement(12708..16226)
                     /locus_tag="IWO04_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0213398.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16519..16977)
                     /locus_tag="IWO04_001796"
     CDS             complement(16519..16977)
                     /locus_tag="IWO04_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0213399.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17275..17793)
                     /locus_tag="IWO04_001797"
     CDS             complement(17275..17793)
                     /locus_tag="IWO04_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0213400.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17795..18412)
                     /locus_tag="IWO04_001798"
     CDS             complement(17795..18412)
                     /locus_tag="IWO04_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0213401.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18409..19263)
                     /locus_tag="IWO04_001799"
     CDS             complement(18409..19263)
                     /locus_tag="IWO04_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213402.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19260..20171)
                     /locus_tag="IWO04_001800"
     CDS             complement(19260..20171)
                     /locus_tag="IWO04_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213403.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20212..21546)
                     /locus_tag="IWO04_001801"
     CDS             complement(20212..21546)
                     /locus_tag="IWO04_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213404.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21754..22584
                     /locus_tag="IWO04_001802"
     CDS             21754..22584
                     /locus_tag="IWO04_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0213405.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22828..23077)
                     /locus_tag="IWO04_001803"
                     /pseudo
     CDS             complement(22828..23077)
                     /locus_tag="IWO04_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23268..23462)
                     /locus_tag="IWO04_001804"
     CDS             complement(23268..23462)
                     /locus_tag="IWO04_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213406.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(23967..24152)
                     /locus_tag="IWO04_001805"
     CDS             complement(23967..24152)
                     /locus_tag="IWO04_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213407.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         7162 a         5256 c         4165 g         7626 t
ORIGIN      
        1 tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca
       61 aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc atctttgaaa
      121 atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct
      181 gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga
      241 tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc
      301 tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc
      361 acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca
      421 tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc
      481 tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa
      541 tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt
      601 tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa
      661 agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga
      721 tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt
      781 cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg
      841 taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac
      901 tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt cctgggttgt
      961 agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac
     1021 ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc
     1081 cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac
     1141 gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt
     1201 cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc
     1261 cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa
     1321 aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt
     1381 tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt
     1441 ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc
     1501 gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg
     1561 taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc
     1621 agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt
     1681 gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag
     1741 gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac
     1801 ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt
     1861 cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc
     1921 cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca
     1981 gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat accagttctc
     2041 tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc
     2101 cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat
     2161 cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa
     2221 aactccttat tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt
     2281 ctttagtccg atactcttta tagtagtccg tatgctaaaa taagtatgta atcgaataac
     2341 gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat
     2401 tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct
     2461 tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg
     2521 cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac
     2581 tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa
     2641 acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca
     2701 atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa
     2761 gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt
     2821 gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat
     2881 gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat
     2941 tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa
     3001 cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca
     3061 taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt
     3121 agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat
     3181 aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac
     3241 aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga gattgatcag
     3301 ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa
     3361 caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac
     3421 ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa
     3481 gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa
     3541 gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag
     3601 gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac
     3661 aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta
     3721 gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct
     3781 ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca
     3841 gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat
     3901 cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca
     3961 ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct
     4021 atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt
     4081 caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag
     4141 aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga
     4201 atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag
     4261 acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga
     4321 cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc
     4381 ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt
     4441 atctttcttc taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc
     4501 catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca
     4561 cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt ggcaatagcg
     4621 gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca
     4681 gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta
     4741 atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg
     4801 cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat
     4861 ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca
     4921 gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc
     4981 tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca
     5041 agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc
     5101 ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc
     5161 ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca
     5221 tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga
     5281 tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa
     5341 gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac
     5401 cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca
     5461 aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt
     5521 ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat
     5581 aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag
     5641 acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt
     5701 gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa
     5761 gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga
     5821 tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg
     5881 attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa aggcaaccaa
     5941 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
     6001 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
     6061 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
     6121 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
     6181 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
     6241 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
     6301 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
     6361 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
     6421 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
     6481 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
     6541 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
     6601 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
     6661 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
     6721 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
     6781 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
     6841 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
     6901 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
     6961 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
     7021 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
     7081 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
     7141 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
     7201 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
     7261 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
     7321 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
     7381 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
     7441 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
     7501 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
     7561 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
     7621 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
     7681 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
     7741 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
     7801 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
     7861 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
     7921 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
     7981 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
     8041 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
     8101 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
     8161 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
     8221 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
     8281 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
     8341 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
     8401 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
     8461 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
     8521 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
     8581 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
     8641 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
     8701 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
     8761 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
     8821 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
     8881 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
     8941 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
     9001 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
     9061 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
     9121 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
     9181 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
     9241 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
     9301 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
     9361 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
     9421 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
     9481 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
     9541 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
     9601 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
     9661 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
     9721 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
     9781 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     9841 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
     9901 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
     9961 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    10021 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    10081 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    10141 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    10201 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    10261 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    10321 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    10381 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    10441 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    10501 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    10561 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    10621 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    10681 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    10741 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    10801 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    10861 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    10921 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    10981 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    11041 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    11101 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    11161 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    11221 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    11281 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    11341 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    11401 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    11461 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    11521 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    11581 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    11641 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    11701 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    11761 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    11821 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    11881 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    11941 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    12001 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    12061 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    12121 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    12181 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    12241 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    12301 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    12361 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    12421 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    12481 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    12541 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    12601 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    12661 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    12721 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    12781 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    12841 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    12901 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    12961 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    13021 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    13081 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    13141 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    13201 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    13261 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    13321 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    13381 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    13441 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    13501 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    13561 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    13621 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    13681 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    13741 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    13801 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    13861 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    13921 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    13981 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    14041 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    14101 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    14161 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    14221 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    14281 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    14341 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    14401 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    14461 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    14521 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    14581 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    14641 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    14701 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    14761 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    14821 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    14881 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    14941 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    15001 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    15061 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    15121 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    15181 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    15241 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    15301 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    15361 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    15421 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    15481 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    15541 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    15601 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    15661 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    15721 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    15781 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    15841 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    15901 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    15961 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    16021 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    16081 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    16141 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    16201 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    16261 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    16321 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    16381 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    16441 caatttccta acacttttgt atagtcattc gttgtaaact tcatctattc tttcatcaac
    16501 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    16561 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    16621 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    16681 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    16741 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    16801 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    16861 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    16921 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    16981 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    17041 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
    17101 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
    17161 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
    17221 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
    17281 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
    17341 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
    17401 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
    17461 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
    17521 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
    17581 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
    17641 gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat tactgataaa
    17701 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
    17761 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
    17821 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
    17881 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
    17941 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
    18001 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
    18061 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
    18121 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
    18181 aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
    18241 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
    18301 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
    18361 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
    18421 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
    18481 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
    18541 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
    18601 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
    18661 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
    18721 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
    18781 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    18841 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    18901 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    18961 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    19021 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    19081 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    19141 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    19201 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    19261 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    19321 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    19381 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    19441 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    19501 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    19561 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    19621 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    19681 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    19741 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    19801 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    19861 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    19921 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    19981 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    20041 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    20101 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    20161 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    20221 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    20281 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    20341 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    20401 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    20461 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    20521 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    20581 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    20641 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    20701 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    20761 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    20821 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    20881 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    20941 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    21001 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    21061 aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    21121 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    21181 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    21241 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    21301 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    21361 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    21421 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    21481 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    21541 tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca
    21601 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    21661 atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga
    21721 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    21781 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    21841 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    21901 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    21961 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    22021 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    22081 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    22141 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    22201 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    22261 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    22321 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    22381 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    22441 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    22501 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    22561 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    22621 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    22681 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    22741 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    22801 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    22861 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    22921 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    22981 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    23041 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    23101 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    23161 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    23221 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    23281 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa
    23341 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    23401 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    23461 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    23521 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    23581 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    23641 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    23701 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    23761 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    23821 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    23881 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    23941 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    24001 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    24061 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    24121 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    24181 ttatagaagg tattgtaaat aataatatt
//
LOCUS       DACECB010000045        24130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECB010000045 DACECB010000000
VERSION     DACECB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.045"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            251..3529
                     /locus_tag="IWO04_001806"
     CDS             251..3529
                     /locus_tag="IWO04_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0213408.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4070..5977)
                     /locus_tag="IWO04_001807"
     CDS             complement(4070..5977)
                     /locus_tag="IWO04_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0213409.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6107..6982
                     /locus_tag="IWO04_001808"
     CDS             6107..6982
                     /locus_tag="IWO04_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0213410.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7062..8036
                     /locus_tag="IWO04_001809"
     CDS             7062..8036
                     /locus_tag="IWO04_001809"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0213411.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8225..9361
                     /locus_tag="IWO04_001810"
     CDS             8225..9361
                     /locus_tag="IWO04_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0213412.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9498..9842
                     /locus_tag="IWO04_001811"
     CDS             9498..9842
                     /locus_tag="IWO04_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0213413.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10113..11399
                     /gene="efmA"
                     /locus_tag="IWO04_001812"
     CDS             10113..11399
                     /gene="efmA"
                     /locus_tag="IWO04_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0213414.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11537..12136)
                     /locus_tag="IWO04_001813"
     CDS             complement(11537..12136)
                     /locus_tag="IWO04_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0213415.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12540..13661
                     /gene="mnmA"
                     /locus_tag="IWO04_001814"
     CDS             12540..13661
                     /gene="mnmA"
                     /locus_tag="IWO04_001814"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0213416.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13907..15328
                     /gene="arcD"
                     /locus_tag="IWO04_001815"
     CDS             13907..15328
                     /gene="arcD"
                     /locus_tag="IWO04_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0213417.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15342..16460
                     /locus_tag="IWO04_001816"
     CDS             15342..16460
                     /locus_tag="IWO04_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0213418.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16520..17812)
                     /locus_tag="IWO04_001817"
     CDS             complement(16520..17812)
                     /locus_tag="IWO04_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0213419.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17962..18522
                     /locus_tag="IWO04_001818"
     CDS             17962..18522
                     /locus_tag="IWO04_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0213420.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18651..19028
                     /locus_tag="IWO04_001819"
     CDS             18651..19028
                     /locus_tag="IWO04_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0213421.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18961..21033
                     /locus_tag="IWO04_001820"
     CDS             18961..21033
                     /locus_tag="IWO04_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0213422.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21244..22191)
                     /locus_tag="IWO04_001821"
     CDS             complement(21244..22191)
                     /locus_tag="IWO04_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213423.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22437..22901
                     /locus_tag="IWO04_001822"
     CDS             22437..22901
                     /locus_tag="IWO04_001822"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0213424.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22963..24006)
                     /locus_tag="IWO04_001823"
     CDS             complement(22963..24006)
                     /locus_tag="IWO04_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0213425.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7853 a         4413 c         5062 g         6802 t
ORIGIN      
        1 aaataaaaag cacaaaataa taaaagaaga gcataaaaca aacgactttg ttttatgctc
       61 ttttttatac gttattttaa aaaaacttat tctttttacg ttaataaatc tctttattat
      121 caacaactta agttatattt tttcttttta taaaatacac ttattataat tttgtttttt
      181 attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg
      241 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
      301 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
      361 gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg aaataccaag
      421 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
      481 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
      541 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
      601 ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga cctattatta
      661 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
      721 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaaa
      781 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
      841 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat
      901 gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc
      961 gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt
     1021 ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc
     1081 cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc
     1141 atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga
     1201 aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat
     1261 gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag atcctcctgg
     1321 gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc
     1381 aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat tgacaattaa
     1441 aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg
     1501 ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac
     1561 gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg aaacaaaaga
     1621 gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg taacaccaga
     1681 agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac aagcattgag
     1741 atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct tcgaaagagg
     1801 attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt tgattgcaga
     1861 gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa atgggatgac
     1921 actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg cagctatgca
     1981 aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg cttatcggaa
     2041 tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag catggcccgc
     2101 gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc
     2161 cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg gttgtatcgc
     2221 gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag gttcgatcgg
     2281 aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag gaattgaggg
     2341 agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt
     2401 gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata gcggaacaga
     2461 tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac
     2521 ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc gaacagagaa
     2581 tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctaccactc
     2641 gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccat
     2701 atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc agcctgttac
     2761 gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac cggggaatcg
     2821 tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta tttttgacgg
     2881 agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac caaatcagac
     2941 aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa
     3001 tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc
     3061 aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt
     3121 tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa
     3181 aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc
     3241 tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt
     3301 gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa
     3361 accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa aaaatggcac
     3421 tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag ctggaggaat
     3481 tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag tggaatttct
     3541 ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat
     3601 agatgtctca cttccttcta tctttttata gatgaggttc tccttgctgc ttttgattgg
     3661 ctgttcttat tacttgatgc aggacagctt tttcacaatc tctccactaa acggtgttca
     3721 atcagctgac cctcggtatt agctcaacaa acggcaaaat atgaggaact atttttccgt
     3781 ttttttctct aatactcagg tcaggacgct gatttcacaa cctctcgata aacggtgttc
     3841 aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaag ccattttcac
     3901 aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat acatggtatt
     3961 ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag aagcgatttt
     4021 cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct taccagattc
     4081 gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca aatgtttcat
     4141 agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg gcatggacat
     4201 cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa atcgttgccc
     4261 aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct tccaatgcac
     4321 agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg tttacttttc
     4381 cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg ttttgaaatt
     4441 gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca aataaagcgc
     4501 cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt ccaccataat
     4561 tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct gccggaaaga
     4621 cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc atttcccatt
     4681 ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg agtttgctga
     4741 aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt ttgaactggt
     4801 cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta tttaatttta
     4861 tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga cgttttttat
     4921 aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct tcgccaaaat
     4981 atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca aggtaatacg
     5041 ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa tagattccgc
     5101 tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt ttaatgatca
     5161 tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa tgatcgaagt
     5221 acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc gtgacaagtt
     5281 ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc ggattataca
     5341 ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc aatttatcaa
     5401 attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg gcaagctcta
     5461 ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg ggatggtctg
     5521 atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag aaggcgttcg
     5581 tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt aatgtatcca
     5641 acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat tcaacttttt
     5701 cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa tcgtttgcca
     5761 gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct ggatgggcaa
     5821 gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa tcttggaaac
     5881 cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct tcattgactg
     5941 cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc cttttttgtt
     6001 tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa aaaattgaaa
     6061 attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga aaaaaaggag
     6121 gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag ataaaaaaat
     6181 taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc tttttttagc
     6241 tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc cggtagaata
     6301 ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga agctgatcaa
     6361 tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc ccgttttgat
     6421 cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc cagaagaacg
     6481 ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta cttacgcggc
     6541 agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc caaatgagat
     6601 tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta gccagatcca
     6661 accactagga cttcctacga ttccttttca ggttagtaca gggatttcta gttttttaaa
     6721 gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc agcgagctac
     6781 aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac ttggcagtac
     6841 atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag gagaacgtta
     6901 ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca gttggagtaa
     6961 cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt ggaaaaatat
     7021 gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat aagtatcagg
     7081 atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa aaaatcgcga
     7141 aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat ggcgagattt
     7201 ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa tcaacgaatg
     7261 cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg aagcgagcaa
     7321 gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa gaccgcacgg
     7381 cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa gaagcagggg
     7441 caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc tttgatattc
     7501 ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa ttaggtctga
     7561 cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt gcacgtagtc
     7621 tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat gcttcaactg
     7681 agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta gatgatatct
     7741 tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac ggcgcaaaag
     7801 aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt attttagaca
     7861 atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa aatcgtcatc
     7921 cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc aaacgcatcc
     7981 atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc gaataaacaa
     8041 aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg cgacaagact
     8101 tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa atgagctctt
     8161 ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg aagggatgaa
     8221 tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc cacaagtaat
     8281 tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca tacatggatt
     8341 tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag acagtctgca
     8401 agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata atacagctat
     8461 cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta cagctatcga
     8521 acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg acgttacata
     8581 tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag cgttacgaga
     8641 agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt tgatgccaat
     8701 caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag atgcggtaca
     8761 agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat taagtatctc
     8821 tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag acggagtaaa
     8881 tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag gtacagaaaa
     8941 tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg aagaacgtga
     9001 agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat tagaagcaaa
     9061 cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg tgttgaatct
     9121 tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta atggggtcgc
     9181 tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg tattagaagc
     9241 aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct ttggtttggg
     9301 aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg tcaaaaaata
     9361 actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat tttttaggga
     9421 acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga gaaactaatt
     9481 ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc gcaccaatta
     9541 gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga tttacagaaa
     9601 caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa agcaaagaag
     9661 gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg tcgatcacta
     9721 cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa atgagccaag
     9781 ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa aaagtcgaat
     9841 gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga tgactaactt
     9901 tgtaaggacg acgcaaatta gttgtctttt ttatttttaa atttaacaca tgttgttatt
     9961 tttaatattt tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa acgattgtta
    10021 tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt gtgcaaacgc
    10081 tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg gtcgttttaa
    10141 cgaattggaa gagaaattat ttgttctttt tatcgggaca gtttttgtca ggaatcacta
    10201 gtatggtcgt ccaatatgcg atcatctggt atttgacaag agaaaccggt tcggcgacaa
    10261 ttttgagttt tgctacttta ctaggaatga ttcccatggt tttactaagc ccatttgtag
    10321 gaccgctagt ggatcgctgg gataaaaaag ctcttttgat tgtcacggat attattgtag
    10381 cgattttcgc gcttatttta gcagtagtcg gaacgatttc agaatccttc ccgatttggc
    10441 tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac attccaaatg ccaacgatcc
    10501 agtcgattat gccgacaatc gttccatctt ctcacataac aaggacgaat ggacagttag
    10561 gaatggtcca atcagcgaat ttcatcattg cgccagctct aggtgcggct ttgttttcag
    10621 tcgttcctgt caattacctt attttattag atgtattggg ggcagttttt ggtgtcggct
    10681 tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt ccgcttacta
    10741 tttttaaaga tgcaaaattt gggctgcagc aattgatgga taataaaggg ttatggtaca
    10801 taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt ctgtatccat
    10861 tgatgacgct agattacttt ggtggttccg ttggacaggc gggagcagtg gaagtagtct
    10921 acgcagtcgg aatgctctta ggcggtgcac tgatcagttt tataggaaca tggaaagacc
    10981 gaatgaaacc tattattata gcctacatca ttatgggcct gacgatcggt gcaagcggat
    11041 tggttccaaa cgatagtcaa gggttcttgt actttcttat cttgaatgca ggagcaggtt
    11101 gtgcaacgcc atatttcaat acactgctga tggcaatgat ccagcagagt tatgaatcga
    11161 atgttttagg tcgtgtctta gggaacttca actctttgat gaacctggca ggtccaattg
    11221 gattattatt tgcaggacca ttagctgatc gtttaggggt agaaaaaatg ttcttgtttt
    11281 ccggaatcgg gattttatta tgcggaatcg ttttattttt aacgtcagct gcccgaaatt
    11341 atgataaaga attacagaaa aaactggtaa aagaacacca tgagcaaaaa gacgaatagt
    11401 atggaatgga gttgtgacag aagtcttgtt acaactccat ttctgatcct aatgttcctg
    11461 tggcaataag cctactcaaa tgaaaaatat gagtaattat ctttatttgt ttaatacttg
    11521 atatagggca gttttttcac gacctatcgt acttcctctt tgcttcatag atttctttga
    11581 tttgttgctc agataacgga gtgccaaatg gtagtttttc ctttaagagt tctctttccg
    11641 gtgcgtctac gcctacattg acatcgttct cgataggtac gctgtaatga tggatatgtc
    11701 catgaagatt gcggatagtg tcgttaggcc cgagcaacat cggataatga gtcaagtaat
    11761 attgttgatg gtcgaattta acgatggcac ctacatcata aaaataaaat ttgggacttc
    11821 cgtcagtaag cctaggatca tttttttcta aataacggaa aaaagcgcga ctgtcatggt
    11881 tccctttgat caagtggatt gttccttgca gttgagtgac cagttccaaa atttctgcat
    11941 ttgctttttc atattgcgga tgcatagcaa ggtcgcctag atgataaaca tggtcggttt
    12001 cttttattac tgcattccag ctagtgatca actgttggtt catttcatcc acatttgcaa
    12061 agagccgcgg agcaaagtcg ttttttccta ataagcgttc atggaaaaag tgcatatcac
    12121 tgataaagta attcatgtat cttttgcctc ccttttttct tagagtaaac aaaatcactt
    12181 atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg cagctttaag
    12241 cgataagaag caattttgaa aaatagtttt tcacaattta taaaattatt ccttattgcc
    12301 gaaaggctgg ccctttttat gagtatgcca attttttata tcgaatgctc aaaagatgag
    12361 tgattttttg gttgactatc atgtttgtgt actgaatagg tccagtagaa cagtattttt
    12421 acgacttgat aattaaggga tgaaattttc agaaatatga cgtataatgt aaaatactga
    12481 aaagttgcga ccttcaactc gtagatgttt cagaatctaa ttgaaatgat ggtgatatca
    12541 tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc gactcatctg
    12601 taacagcact tttgttaaag gaacaaggat acgacgtgat cggaatattc atgaaaaatt
    12661 gggatgacac ggacgaaaac ggtgtatgca cggccacgga agattataag gatgtagcga
    12721 aagtagctgc tcaaatcggg attccttatt actctgtgaa ttttgaaaaa gaatattggg
    12781 atcgtgtatt tgaatatttc ctagccgaat accgcgcagg acgtacacct aacccagacg
    12841 tgatgtgcaa caaagaaatc aaattcaaag cattcttgga ttatgcaatg gatcttggcg
    12901 ccgattatgt agctactgga cattatgcgc aagtcactcg cgatgaaaac ggcgtggtcc
    12961 acatgcttcg tggagtagac aataataaag atcaaactta cttccttagc caattatcac
    13021 aagaacagct ttcaaaaacg atgttccctc taggcggcat ggaaaaatca gaagtacggg
    13081 caattgctga acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg ggaatctgct
    13141 ttattggtga aaagaacttc aaacaatttt tgagcaacta tctgccagca aaaaaaggaa
    13201 atatggtcac tcttgatggt gaagtcaaag gacaacatgc tggcttgatg tattatacga
    13261 ttggtcaacg tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa ccctggtttg
    13321 ttgtaggaaa agatctagcg acaaacacat tatatgtggg gcaagggttc catcatcctg
    13381 cattgtatgc cacaagcttg gatgcaagtg aaatccactt tacgacgaat gaaccaatgc
    13441 caaaagaatt caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt cctgtgacag
    13501 ttcgcttatt ggatgataat cgtgcagaag tcgtattcga cgaacctgta cgtgcaatca
    13561 ctcctggaca agcagttgta ttttatgatg gaatggaatg tctgggtggt ggtttgatcg
    13621 accacgcata tcaagaaaca aaagttttac aatacgttta agcgatatta aatgaatcaa
    13681 aacagctatg gctaacaaaa gaaacacctt ttcttttgga tagcatagct gtttttttgc
    13741 aataaaatta atttttattc attttatact tgaaatactt gtgtaaaaag ttcataatat
    13801 ccaataatat gaataaagtt gctttttgtt aagcaagagc agttgcaaaa tagaagggaa
    13861 tcttttgcaa gtgatttgtt caattttata agtgagggtg atggaaatgg aaaagaaatc
    13921 gacgggtatt agcaaatggg gactggttgc tctggttgtc agttcgtcaa tcggaagcgg
    13981 tgtgtttggg atcacaagtg atctggctag ttctgcagct ccagggccag cgatcctttc
    14041 atggataatc gttgggattg gtattttagc tttggtatta tcgctgaatc atttaggaga
    14101 aaagcgtcca gatctagatg gtggtatttt cggttacgcg gaagcttcat ttggtaaatt
    14161 aggtggcttc atcagtggtt ggggttactg gttgtctgcg tggctgggaa atgttgcttt
    14221 tgcaacgatg ctgatgagcg ccatgggaga atttttcccg atattcaaag gtggacaaaa
    14281 tgttccttct attttatttg caagtatttt tatttggggt ctgactttat tagtaaacaa
    14341 tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat tagtgccatt
    14401 attcctattt ttaatatttg tatttatcgc atttaaagtg aatatattca ctgctgattt
    14461 ttggggtaac gtatctgata atctagtgaa tggaacaggg cagcaaggaa cgatctggtt
    14521 gcagattaaa ggttgtttga tggttatgat gtgggtcttt gtagggatcg aaggcgcaag
    14581 tgttttagcc aaccgcgcga agaaacgttc agaagcccag caagcatcga tcattggtct
    14641 gctttgttta ttgtttatct atatccttgc ttcgatcttg ccatacggcg tcttatctca
    14701 agaagagtta gcaactattt cacaacctgc tatggcaaat attttaaaag gaatcgtggg
    14761 aaattgggga gcagcgctga tcaatatcgg cttgattatt tcgatcattg gttcttggtt
    14821 gtcttggacg atgctcccag cagaaacaac gatgttgatg gctcgagacg gtctgttgcc
    14881 gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact tattcgttag ttgttactgc
    14941 aatcttgact aatctattct tattgacttt cttagttact gattatgcct atcaatttgc
    15001 ctattcttta tgtacagcgg caattttgat ttgttatcta cttgtcggga tctatcaagt
    15061 ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga ttgctgtagt
    15121 ttttgaattg atgggcatca ttttagcagg attttcttat gtccttttat gtagtattgc
    15181 ctatcttcct ggtttttgct tctactggat cgcttgtcgt gaaaccaaac atcaaattac
    15241 gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt caatcgggtt
    15301 gcttgctgta ggctggatca agatataagg gggaaagaaa catgaaaatc gcaaatttcg
    15361 gtgtagaaga atggctaaat aaatgggaaa agaaagcggt atatgatatt gcacaaagct
    15421 cgatcgaagc attgacatta gacgaactcg ttggcttaga tgggactagc gtaagtaaat
    15481 ttttcgaaaa acgcaaaaac gaaccattgg actatggctg gatcgaagga tcagatgttt
    15541 tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc ttacaaacga
    15601 atggagcgac tggtgctaat cttttagctc tatacgcgtt agtggaagca ggagaccatg
    15661 ttgtttcgat gcttcctact tatcagcaat tatatgacat ccccaaatct ttaggtgccg
    15721 ctgttgactt tgttcatttg aaggaagaag aagattggca gtttgattta gaacagctag
    15781 aagccttagt caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac aatcctacag
    15841 gcacattgct agatcggaaa actttagaaa aaatcgcaga aattgctcgt acagtagatg
    15901 catatgtatt gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa ttcttgtcga
    15961 tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc tactccattc
    16021 caggtattcg tattggctgg acagctactg gacctgagtt ggcagaagtt tttcgaaagt
    16081 atcgagacta cacgatgatt tgcggcggag tcctgtcaga tgatcttgct gtacatgcat
    16141 tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat cattacagag aaccttgtga
    16201 ttttaaaaca atgggtagcc aatgaaccga aagttgaact tgtcgctccc aattacgtct
    16261 ctacttcttt cattaaatta gccatcgaag aagatgacca gacattttgt atcaatttat
    16321 tagaagaaac aggagtacta ttagtaccag gaagtgcctt tgatttaccg aagcatgccc
    16381 gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg attattcctc ttatctgagt
    16441 tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca actgttcaag
    16501 cagtgttttt catttacgct tattgtctat gagaaggaga gtgccctgaa gctatggaga
    16561 ctcctgaagc tttttcttct gcagtttctt gctgatggat ctcttgtgta ttgtgtcgta
    16621 caagcaagaa gttgaccaaa acaagaagag aagtcgaaag aaagacgcca cgataaccaa
    16681 atgcactgga aacactggag ccgatcatag gccctactac gttaccagtt gcttggaaag
    16741 attggttata actgaagatc cgtccagcag cttgatgagg cgaatatttt gtgatcaatg
    16801 cttggacagc agggagcaga caagcatcag agataccaat caaaaaacgt aaggcagcta
    16861 attgccaaac attcgtgacg aatgccattg ggatataaac cagaatcgca aaacccaaac
    16921 caatcgcaag tattctttca ctcccaatct tgtcacctaa gcgaccaaat cttggagcgg
    16981 ctatcaaagt ggcaatccca ggtatcgaag caatgattcc gctgatcaac gtaacattcc
    17041 catgtccatg cagcaattgg cgaatgtaca gactgatgat tggactaatg gaattgttgg
    17101 atgcttggat gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga tatttcaagt
    17161 ctttgaaaat ctgtttggca gaaaccatgt tttctttttt cactgggaca aatttttcat
    17221 ggacgaaaaa cagactcaac aagaagacga aaaataagat gatccctgta atgaaaaagg
    17281 tagggcgata gccaaacgct gaagcagaga tcccgccaag aagcggtcca agtaatgttc
    17341 ctgtaacaga accagttgct aaggttccaa gaacttgacc acttttttct tttggcgtcc
    17401 cagttgcgac aagggcagtg gcattactga tataaccaga aaaaatccct tgaagcagac
    17461 gcaatgcgac taattgatac acactagtca cgcaacccat caaagaaatg acgattgcca
    17521 ttcctaatga agcacgcaac agcatcaatt ttcttccttt ttggtcggct aattttcccc
    17581 accaaggaga gataagcgta gtcaccaaga aagtagatga aaaagtcaat ccgctccaaa
    17641 agttcaattg tgacgtacta taattaccta acgtatctat gtaaagtgac ataaaaggca
    17701 tgactaaact aaatccgata ccagccataa atgttccaaa ccataagaca aacaagtttt
    17761 gttcccaagt ttctcttttc ctaaataatt tttcctttat tcttgcagcc aattagactc
    17821 cttctttctg aaaattttca tctacagctc tttattataa actacaatat gtagtttatc
    17881 aatgaaagtc tcttttttct ctttcttttt cgtggtaaac tgaaagagtg aaggatttat
    17941 tttatgagag gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa aatcatcgac
    18001 tgctttcgag aactagcaga agagatggat gttcagcaaa taacctttca gcatttagcc
    18061 aaacgcttgg atatcaaggc tccctcgctg tataaccatt tcaaaaatat tcgtgatgtc
    18121 aaaacagcgc taacagcgaa acttctgcag gaacttaatg accagctgcg acgggctctt
    18181 gtgggaaaaa gtggtcagga agcactgcag atctatgcag aaacttatcg atcctttgct
    18241 ttttcaaatc aagcagtgta tgagttactg atcagtgtac cgcataccaa tgaagctgtt
    18301 ttattagatg gaatccatga aacgaatcag atcattttgc agatatttga ggcttttcaa
    18361 ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc gtagtatgat ccacggttat
    18421 ctttccttac gttttttagg atactttaca aaagaaccaa caatcgatcc agaagacagc
    18481 tatttttgga tgatcaataa ctttatcgct actttgcctt aaacaatcag attcatttgt
    18541 aacaatttta agatgttcat taaatgaccc ttttataaga gaaaattctt gcaactttct
    18601 tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag gagcacgagt atgtatcaag
    18661 taattaccat gttcggggac aatgagcctt ggtggttttt tgaagactgg gaagaagata
    18721 ttgaagaaga agaaacattc gagtctttcg aagaagcacg acaagcgtac gaaaaacaat
    18781 ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt ctcagcgcct
    18841 tttggaatga cggagatgag cgctggtgtg aagagtgtga cgatgattta cagcaataca
    18901 agggacttgc gttgttgaaa aatcatcagc ccatcacagt agaaagcaga aaggaatttt
    18961 atgaaacaac taattctagc ggaaaaacca agcgttgcga aagacctaag caaggtgctt
    19021 ggtgctaatc aaaagcacaa aaattattat gaaggaccca aggtgatcgt tacttgggcc
    19081 cttggtcatt tattaggatt gaaaatgccg gaagatctga ataaggaatg gcagagctgg
    19141 caaatggaca cgttgccaat gatccctaaa aaactaggaa tcaaaccact tcctaaaaca
    19201 ggacatcaat taaaagcaat caagcagctg gctcagcgaa aagatgtctc agaagcagtc
    19261 attgcgacag atgccggaag agaaggggaa cttgttgctc gctggatctt agagtatgtg
    19321 catttcaata aaccagtgaa gcggttatgg atctcttctc aaacagataa agcaatcaaa
    19381 actggattca agcaactaag acctgcgaaa gaatatgatg cattgtatga atctgctctt
    19441 gcccgggcaa aagcggactg gctggtagga ttgaatgtga ccagagcctt aactgtgaag
    19501 tacgcggata atttatcagc aggccgtgtg cagacaccga cactagcatt agtgagagac
    19561 caagaaaaga aaatcgagca gttccgtcca caaacgtatt tcacgatcac gttgcaagct
    19621 ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac acaagaagaa
    19681 gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga agtccaagaa
    19741 aaattaaaaa cggaatctgc gccattgcct tatgatttaa cagagatcca aagagaagcc
    19801 aatcaacgct atggctattc tgcaaagaaa acattaagtc ttgtacaaag tttgtatgag
    19861 acccataaga tcgttactta tccgcgtaca gacagcaaat atctgacaaa tgacatgaaa
    19921 agtacgatga aagaacgatt gcaagcagtc agcgactttg ctcctgaggt caagcagtat
    19981 ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc aagacagtaa agtgaccgat
    20041 caccatgcac ttttgccaac tgaaaacaga gcacgttatg aaaaattaag taatgaagag
    20101 cgaaaaatct atcaaatgat tgtctctcgt tttttaggac tgtttgctca accccataaa
    20161 gtcagccaaa caaaagttac ggttgaattt ggtaaagaac gattcatttt tcggcaaaat
    20221 cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg agacggaaac agtcaaatgg
    20281 gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa aagaactatc tgcaccgcct
    20341 aaaccattgt cagaagcaag cttgcttggt caaatggaaa aatacagtct gggaacccca
    20401 gctacgcgtg cagagattat cgagaaacta atcaaatctg aactgatgga acgtacccct
    20461 caaggattgc aagtatctcc taaaggtaag caattattag tattggtaaa tccctcttta
    20521 gtaacaccag atttgacaga aagctgggaa aaagaccttg aagctattgc tgcaggaaag
    20581 aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt ggtgaatgag
    20641 atcaaatcaa gcaaacaaga atatcaagat ttctcgatta cccaaaagaa atgcccagaa
    20701 tgtggggcca atctgcgcga gaaaaacaca cgtgacggca aaatctatgt ctgcaccaat
    20761 tcagattgca actaccgcag aagaaaagat ccaaaagtct ctaatcatcg atgcccgcaa
    20821 tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg cttatttccg ttgtaaatac
    20881 gatggaacga cagaaaaatt gcttgataaa aaagagcaga agaagaaaat gaccaaacat
    20941 gaagaacgac gcttgctgaa aaaatatgca caagcagaag aaccagaaga aagcccatta
    21001 gcagcagcac taaaagcagc aatgaagaag taataagata tgaaagaggt tgtgatagaa
    21061 gtggtcagct tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa
    21121 atttcggctt atttccgaag aagctacttc tggaacacca tttattcgga aaaaaggact
    21181 gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa atgtttactt catttgacgt
    21241 atattattgg tgaggcttca attcatttgg cttcactttt tctgtttctc ttatgatata
    21301 aacaggacgc tgtttcgttt ctaagaagat tttcccaata tatttcccga tgattccaag
    21361 acataacaat tggattccac caatgaacag gaagatcgag accatggaag gccatcctga
    21421 tgtaggatca ccaaacaaca atgcccgcaa aataatgaac aaaattgcaa taccagaacc
    21481 gacacaagac aaagcgccga taaatgaagc aatcgttaac ggtgcatccg agaagttcac
    21541 gataccatca atcgagtagt tgaataattt ccagaaggac caagatgttt cgccagccac
    21601 acgttcgcgg ttttcataag aaatatattc tgttttaaat ccgacccagc taaataatcc
    21661 ttttgaaaat cggttgtatt ctgttaattc taggacggca tccaccattt gacgagtcat
    21721 caagcggaaa tcacgagcgc catcgaccat ttctgtatcg ctgattttat tgatcaactg
    21781 gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg ccttcacgag tcgaacgccg
    21841 ggtgccgaca caatcgatat cagaagtagt gatccggttg atcatctcag gaagcagttc
    21901 gggcggatct tgcagatcaa catccatcac ggtcactaaa tctcctgtag aagcttgcaa
    21961 tccagcgtac agagccgctt ctttcccaaa atttctggaa aatgagacaa agcggacata
    22021 aggataagct gaggctagct tcttgattgc tgctaacgta ccgtcttttg atccgtcgtc
    22081 aataaacaaa tattcgaact catgggcacc tttgaatttt tcaacctcat caaaaaaaag
    22141 aggaacagtt ttttcttcat taaaacatgg gacaacaatt gatactttca tcgtttctat
    22201 cctttcaatc gtatgtttat atatggttta cattcaaaat ttaataaacc ttaaataaca
    22261 cctttaaaat ttgattttac gtaaaagaac aggtattctt tttgtaaact aacgtattta
    22321 gtttaccatg tttcctcggt ttatcaaact tttaagaaca atttttatta gttggcaagt
    22381 agttttggat agagaaaaag gaggaagttc cagtctattg actaggagca aacaaaatga
    22441 aaatacacac acctatcggc tttttatggt tagatgcaga tgaatccgga ttgactgctg
    22501 tatcttttca gccaatttca gaaagcgaat cagcgaatca gcgaatttta acgcgcacac
    22561 aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga atttgatgtt cctttatcta
    22621 taaagagagg tacgcctttt cagcaaaatg tctggaaagc tttgtcgacc attccttatg
    22681 gagaaatccg aacgtataaa gaagtagccg ttgctgttga tagtccgaaa gctgtgcgag
    22741 cgattgggca ggcaaatcga accaatccat tgccaatcgt tattccttgt catcgagtga
    22801 tcgggcaaaa tggaaaactg acaggatata tgggaaacgc aatagatggg gttgaaatca
    22861 aacgccaatt gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac
    22921 attgttccat gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca
    22981 aataaatagt aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa
    23041 gcgtgtacct tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc
    23101 agtgatctca aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga
    23161 aaacaagctg ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa
    23221 ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat
    23281 gaatttttca aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag
    23341 catggcgaat tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac
    23401 agattggagt gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg
    23461 taatccaaga aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat
    23521 gatctgtcct ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga
    23581 agaaccacaa acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag
    23641 ttttttacca atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac
    23701 aattcctttt acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa
    23761 aacagagaat tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg
    23821 tttaaaaaaa gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa
    23881 ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa
    23941 accgggtaag atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga
    24001 tttcatttga tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca
    24061 ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt
    24121 gtttcgaata
//
LOCUS       DACECB010000046        23783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECB010000046 DACECB010000000
VERSION     DACECB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.046"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(9..743)
                     /locus_tag="IWO04_001824"
     CDS             complement(9..743)
                     /locus_tag="IWO04_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0213426.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1070..1480
                     /locus_tag="IWO04_001825"
     CDS             1070..1480
                     /locus_tag="IWO04_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0213427.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1473..2174
                     /locus_tag="IWO04_001826"
     CDS             1473..2174
                     /locus_tag="IWO04_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0213428.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2174..3760
                     /locus_tag="IWO04_001827"
     CDS             2174..3760
                     /locus_tag="IWO04_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0213429.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3779..4000
                     /locus_tag="IWO04_001828"
     CDS             3779..4000
                     /locus_tag="IWO04_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0213430.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3997..4758
                     /locus_tag="IWO04_001829"
     CDS             3997..4758
                     /locus_tag="IWO04_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0213431.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5138..5647
                     /locus_tag="IWO04_001830"
     CDS             5138..5647
                     /locus_tag="IWO04_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0213432.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5722..6261)
                     /locus_tag="IWO04_001831"
     CDS             complement(5722..6261)
                     /locus_tag="IWO04_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0213433.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6401..7012)
                     /gene="rpsD"
                     /locus_tag="IWO04_001832"
     CDS             complement(6401..7012)
                     /gene="rpsD"
                     /locus_tag="IWO04_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0213434.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7328..8125
                     /locus_tag="IWO04_001833"
     CDS             7328..8125
                     /locus_tag="IWO04_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0213435.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8153..8788
                     /locus_tag="IWO04_001834"
     CDS             8153..8788
                     /locus_tag="IWO04_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0213436.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8808..9581
                     /locus_tag="IWO04_001835"
     CDS             8808..9581
                     /locus_tag="IWO04_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0213437.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9989..10786
                     /locus_tag="IWO04_001836"
     CDS             9989..10786
                     /locus_tag="IWO04_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0213438.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10764..11177
                     /locus_tag="IWO04_001837"
     CDS             10764..11177
                     /locus_tag="IWO04_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213439.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11161..13806
                     /gene="mutS"
                     /locus_tag="IWO04_001838"
     CDS             11161..13806
                     /gene="mutS"
                     /locus_tag="IWO04_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0213440.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13824..15932
                     /gene="mutL"
                     /locus_tag="IWO04_001839"
     CDS             13824..15932
                     /gene="mutL"
                     /locus_tag="IWO04_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0213441.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15954..16514
                     /locus_tag="IWO04_001840"
     CDS             15954..16514
                     /locus_tag="IWO04_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0213442.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16599..18587)
                     /gene="treP"
                     /locus_tag="IWO04_001841"
     CDS             complement(16599..18587)
                     /gene="treP"
                     /locus_tag="IWO04_001841"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0213443.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18810..19523
                     /gene="treR"
                     /locus_tag="IWO04_001842"
     CDS             18810..19523
                     /gene="treR"
                     /locus_tag="IWO04_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0213444.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19600..20046
                     /gene="msrB"
                     /locus_tag="IWO04_001843"
     CDS             19600..20046
                     /gene="msrB"
                     /locus_tag="IWO04_001843"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0213445.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20216..20818
                     /gene="ruvA"
                     /locus_tag="IWO04_001844"
     CDS             20216..20818
                     /gene="ruvA"
                     /locus_tag="IWO04_001844"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0213446.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20831..21832
                     /gene="ruvB"
                     /locus_tag="IWO04_001845"
     CDS             20831..21832
                     /gene="ruvB"
                     /locus_tag="IWO04_001845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0213447.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21861..22439
                     /locus_tag="IWO04_001846"
     CDS             21861..22439
                     /locus_tag="IWO04_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0213448.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22491..22973
                     /locus_tag="IWO04_001847"
     CDS             22491..22973
                     /locus_tag="IWO04_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0213449.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23090..23464
                     /locus_tag="IWO04_001848"
     CDS             23090..23464
                     /locus_tag="IWO04_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213450.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7767 a         4278 c         5108 g         6630 t
ORIGIN      
        1 gcttttgatt aagccataaa taataattta tataagaata ctgctacgac accagcacag
       61 ataggtgcta caactggtac ccatgcatat ccccattgag aagaaccttt atgtttcaat
      121 ggaagaagtg cgtgtacaag acgtggagca aagtcgcggg ctgggtttag tccaggacct
      181 gtaggaccac caagtgaagc aactaatgtc catacaagga aaccaagtgc taagtgagct
      241 gtaccaagat tatcttggaa gaatggtgct tttgtaatac ctagggcacc aaagaataaa
      301 acgaatgaac caaagaattc attgacgaaa ccattgaatt tagaaccaac gctgttgatt
      361 gttgagaatg tacctaacac atggtttacg tcttctgttt ggtcgtagta aggtttgtaa
      421 caagctacta cgatcaattg tccaatcgct gcaccaatca attgtgcaat gatataagga
      481 acaacttcag cccaagggaa tagaccatta gatgctaaag caattgtaaa tgctgggttg
      541 atgtggttac cgctgattcc accaaaaatc attgctggca tcataacccc gaatccatac
      601 ccgacagcaa tcagcatcca gtcactgcca tatccttttg taccttttaa atctacgttt
      661 gctacagcac cgttacctag aacgattagt aatgctgtac caatcatttc cgccgcaaga
      721 cgggtcatta gtgagtaatc cataaatacc tccaaaagtt ataaaaatgc aaaaaaaaaa
      781 gaaacatcaa cacgaaaagc atcggtgcag tgtttcgtgt cggaaggatt tctgcagtct
      841 taacaaatgt gaataaaaaa atgaattcca ttacttataa gaaaccagac caacagtaaa
      901 actccaactc tttttaaaag ttctccacaa atcaccaagc ttaactataa atataccaaa
      961 aaaaaaacgt tttttttctc aaaaattgta tcttagaaac agcatgtttt gaaactttca
     1021 gaaaaggttt gttagtaaaa tgaaagctat acgtaaggag tgttgtttga tgaaattttt
     1081 tcaagagttt ttcattatta ttttcttttc tgtattagga gaaggcatac ggatttttgc
     1141 tcatttacct attcctggaa gtatcttagg tattcttttt ttatttttag catttgaaat
     1201 gcatcttgtt gatcctgaaa aaatcggagc aactggtgat tttttattga ataatctagc
     1261 cattttattt gttcctgctg gtgtaggttt attggaatat tttgatgata ttgcttcgat
     1321 atggcctgtt ttactgggag cagtcgtcgt gtgttccatc gtcacgatgg ttgcagcggg
     1381 aaaaacggca gaaggtgtag aagctttgct gggctatgtc cgtaagaaaa aagcagaaag
     1441 ggtatctgta aacgaaatgg aggagaatca aattgactga gttttttgtg aatccattat
     1501 ttggcttaag tttgacttta ggagtgtacg tattatctga caaatttttg cggaaatata
     1561 aactgcctgt tttgaatcct ctcgtattat cgattgttgt tattatctta tttttgcatt
     1621 tttctgggat cagctataaa gactataata tcgggggcaa ttttttgact atgctgatta
     1681 ctcctgcgac agtggcactt gccatccctc tttataaaaa cttccgttta ctgaaagaga
     1741 atttttatcc tgtaatggct gcgattttag caggaatagt cgcaaattgc ttggttagtg
     1801 tagggttcgg ttacttcttc tctcttcata aagagatggt gatctcatta ctacctaaat
     1861 ctgtaacgac ggctatttct gtcgatttat ctcatacgat gggcggggtg aatgccgtga
     1921 cacttgctat tgttgtctct acaggtattt ttggttcgct gatcgccact catattttcc
     1981 gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc gttaggaagt acttctcatg
     2041 ctattggtac agcaaaagca atcgagattg gtgaaataga aggaatcatt tccggacttg
     2101 cgatatgcgt caacggaata ttgaccgttt tattgctacc gttattcttt cagccttttg
     2161 ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca ataaccaaaa cgttcgagca
     2221 taaaaaagga gggaaagacg tgtatttgtc ttccgagtat cttaaacaat ttgataaaga
     2281 tctatattat aagattctcc tcctagaaag ttttgaggat caagcttggc ataccgctgc
     2341 acaattagcg caagttgttc agctggatgc ccgcagcgtg tcgaaatacc tgaacgagct
     2401 gtctaaaaac tatcagcagt ttagcggtaa gacacaccct ttgtttacaa aaaatcaccg
     2461 ttctggttat aacttttatg atacgctcga ttcaattgaa catgagcgtt ttttgatcta
     2521 tctcgtgcaa agtacgctga aattccagtt gctgcacgac attttttttg aagaattcca
     2581 tacaatgtac caatttgcgc aaaagcatta cattagtgaa tctactgctc atcgaaaaat
     2641 caatgaatgg aaacagcaac tgcaaactta cggaattcgg ctgcaacgag gtacatatat
     2701 cgctcaaggc gaagaagaaa tcattcgact ttacttgcat atgacattct ggcagttgtt
     2761 tcgagggaaa atctggccat ttgaaacgat ttctcaaatg gacgtaaaga atatggcaga
     2821 acatatcatg gcttttttta atgttcgtct gaacgagatc aaaaaaagac gattggaata
     2881 tatgctaggt gccttttttt taagaaaatc acagaagcat tatgttgtat tgaatgagaa
     2941 aaaaagacgg ctgatttctg acaatctgtt gttccagcga ttttgccagg tcatggagcc
     3001 ggtatttcct aattattttc aagtagaaga cgagttaggc gcattatttc ttgtactgat
     3061 gactcgtgaa gaatattata gtgatcctaa gatacggaag aaaatttttg attttcatca
     3121 acaagcaaaa acaccgccct ttacagccct ttcagaagca aaagccgctc tttctcttta
     3181 tcaggaagag caggggctgc cagcagaaaa tctaaccttc gaggcagaaa actatttatt
     3241 cagcagtcat tttttcgcct atctttttcc taatgcgaaa gaaacgattg atggcaatag
     3301 tagtgatttt attaatcatt tagttattga aaacaaggaa ttgaaacaat ggctcgtaca
     3361 tttttttgaa agtagacaca aacatcccaa tcatctggca tttaaaaatc atgcgttttt
     3421 gatggggcgg tatctgactg tgtttaaaac attaggagcc tttactccac agcttccaaa
     3481 gatcacgatc cttttaatga cagatttccc attatttgaa gagcaactgt tagaggaggg
     3541 attacggaca tttttccgta atgagtatca gctgattttt ctgccaacag attatcgggg
     3601 gagagaagtt gatcttctga tttcgacaag taaagtccac cgcaaaccat gggcggatct
     3661 tgactatttt attgtgactg aagaactaaa attaattgat tacatccaac tatctcaaaa
     3721 atttgaaatg attcaaaaac aaaaactatt aaaacaataa caaagaagga gaaaaaagat
     3781 gacatttgaa gaaattttac cagaattgaa aaaaggtgca aagattattc gtaaaggatg
     3841 gagtggattt gaactttatg ttgttctcgt atccgatgat gaatttgatg gctgtccggt
     3901 gacgccgtac ttattgatca aaacaagtga cgaaggtttt tccagcttcg ctcctacagt
     3961 ttgtgatatt ttagcagatg attgggcgat cgtagaatga aaaaccagta tccagatcta
     4021 gctaaacagg ttgtatttat cacaggagct gcttctggaa ttggtgctgc gcaagcccga
     4081 gcgtttcttg aacaagatgc ttttgtattt ggattagatc aaaagttcgg taaaatggaa
     4141 gaaatttctt tgcgatttcc tgaaactttt gcctacgcac taggtgatgt tcgacaaatg
     4201 aaagaactcc aacaagcggt atcgaaatgc caaaatcatt ttggtgaagt tacgatttta
     4261 ctgaatacag cggggatatt ggatgcctac aaacccttga tggaaactga cgaatcgctg
     4321 tgggatctga tttatgagac gaatgtgaaa agcatgtatc agctgacaaa aatcatcctt
     4381 cctgatatga tccgccaaaa aagcggaacg atcatcaata tggcttcgat tgctggcttg
     4441 attgctggtg gaggtgggat cgcttataca agcgcgaaac atgcgattgt cggatttacc
     4501 aaacaactcg ctctggatta cgcaggacag ggtatccgag taaaaggaat tgctccagga
     4561 gccatccaaa caccaatgaa tgcggctgat tttgaaggga aaggtgagat ggcagagtgg
     4621 gtggcaaatg aaacaccagt caagcgatgg gcacagccgg aagaagtagc tgagctgacg
     4681 ttatttcttg cctctcctca agcaagctac atacaaggaa cgatcatccc aatcgacggc
     4741 ggatggttgt taaaataacc ttgtgttttt ttcaagcttc tgtatgataa aactaaagag
     4801 agaaacaacg gttgatgata aaaaaagtcg atgactgttc ccgacattcg atgccgaaca
     4861 gccagttcaa aaaattcttt ttaataatcg ttaatattcg aaactaagcg gcttttcacg
     4921 acctcttgat ataaacgata ttcaaaagct tgcaactgcg gtaacaagtc cagccaaatg
     4981 aaaaatataa ggagctattt ttatttgacc attcttatta cttgttgcag ggcagctttt
     5041 tcactacctt ctgcgacact tcagttggaa ttttcttccc atcctttggt cgcggtcatt
     5101 accaagataa aattatcttg caaaggagaa atagaacatg gaaaaaaata atacccgttc
     5161 agccaaacgc tggacaacga cagatgtgac tcggatggcg ctagtcactg gtttatatgt
     5221 ggcagtgacg ctggttttgt ctgtctttag ctttggagtg attcagattc gtctctcgga
     5281 gatgttcaac tatctgtcct tgtataacaa aagatacatc gtcgcggtaa cagcgggagt
     5341 agctttggct aatattgctt ctcctcttgg actgatagat gttgcagtag gaagcgtcag
     5401 cacgctgctt gtattgctta tcaactataa aatcactagt cgcatcaaaa acatgaaaat
     5461 caaaatggcc gtgacagcat gcgtatttgc tttttccatg tttacagtag ctggtcagct
     5521 aacgatttta tatcaactac cttttttctt gaattggtgg gtgattgctt taggagaatt
     5581 ggcatcaatg attgtcggtg ggataattat ttattggatc ggtaaaaaag tggatttgtc
     5641 aaaataaaga tctgtttaat ttaaaatagg gcagcatgga agagactgaa tgttgagtct
     5701 tcttccatgc tgcttttttg attaatttgt atgaagaaat cgatttagcc gttttttcat
     5761 caagataccg atataagctg ctccagttgc cttgattgct tccggcagta aaaacggcag
     5821 tagtccactg gcaaatgcag ttgtaaaagt aagatcacca cttattttca gccagatcgt
     5881 tccgcagatc aatgcagcaa gagagcccat tagattggca aagattgcat ataagtaatg
     5941 agcatccgtt ctttcgatca tccaaccagt tatccacgtt tgtaagagaa atccaatcaa
     6001 atatccgcca gttggcccaa ataaaacagc tatacctcca gtcatccctg aaaatacagg
     6061 aagaccaatc aagccaagta aaagataaac tccaacagat atcaatccta gttttcttcc
     6121 gagaaaagtt gctgttaatc caatagcaaa tgtttgccct gtcaaaggaa ccaaaccaaa
     6181 aggaatggtc atctgagaaa ggacagaaat aatcgcggca aattccgcag caagtatcaa
     6241 ttctttcaat gttcggttca taacgccctc ttttcgttaa cttaatattt ttatttggtt
     6301 aacgaaattg taaaacaaag tgattacttt gtaaatagaa aatataaaaa aagaccagct
     6361 atccaataga tagcggtctt tttcaagaaa gcttttgaaa ttataagctt tggttgtagt
     6421 attcaacgac aagagcttcg tcgatttctg gatatagttc gtcacgttct ggtaaacgag
     6481 tcaatgaacc ttccaatttt tcagcgtcaa agcttacgaa tgctgggcgt ccaacagttg
     6541 cttctacagc ttcttttact gttacgatgt tttgtgattt ttcacgaact gagatcactt
     6601 gacccacttc tacgtggtat gaagggatat caacgcgttt gccatcaact aaaatgtgac
     6661 catggttaac taattgacgt gcttgacgac gagtcgttgc aagtcctaaa cggtaaacaa
     6721 cattgtctaa acgttgttct agtaagatca tgaagttaac accatgttta ccttctttga
     6781 ttttgctagc ttttacgaac aagttacgga attgacgttc gttcattccg tacatgtgac
     6841 gaagtttttg tttttcagtt aattgcatgc cgtattctga ttgtttgcca cggctattag
     6901 gtccatgttg acctggtgcg taagggcgac gtgctaattc ttttcctgtg cctgacaatg
     6961 agatgcctaa gcgacgagaa attttccatg atggtcctgt ataacgagac attaaaattt
     7021 cctccaatga atataagttt ttggagtaaa ataatccgtt gaaaaattca taatcatata
     7081 gtttgttctt caatcttcac ctttgcagcc gcggttacac aatcgaaccc gagacggggc
     7141 aacaaactag ggatgagtta ttatttttct gctgcattat tttacacaaa ttacagtata
     7201 tgatagatca tccacgaatg tcaagataat tagtacataa aaacgcttta caaacctagg
     7261 ctgtctttat aaaatgagag ggtatgtgca aatggaaaaa agcgaaggaa gtttaaggag
     7321 aaaatcaatg aatccggtat caccattatt tgagaaaatc gatgcttcaa ttgaaaagaa
     7381 ggcgaatctt tttgaaaaca gcttttcacg ttacgctgtt cgagcggttc tcgcctgttt
     7441 gtttttgact ttaggaacgg ctgtggcatt tgcgatcgcg atgaaggggg aagatatcgc
     7501 gcatggtcta ggaaagattt tatacgcatt tatgttcagc tggggtctgg tcatgattct
     7561 atacatgaac gccgaattag gaacatcgaa tatgctttat atgaccgtgg gtgtttttcg
     7621 gcagagactt gctttaagta aggcagccaa gattctattt gcctgtattt ttttcaatat
     7681 gattggcggt atattatgtg gatttctaat ttctatgact acgccatttc aagatttaac
     7741 gttggacaac tttatgttag aatcaatagc agtaaaacta gcaaaacctt cagcaacgat
     7801 tttggtggaa gggatgtttg cgaatatcgt tgtcaatacc gctgtactga tcagcatgcg
     7861 gatgaaagag gatgtcggaa aagtgatcac agtcgtattc atcattttca tttttgcttt
     7921 tttaggatat gaacacgtga tagcgaattt tcctgccttt acgcttactt actttgcttc
     7981 tcacggacag atggatggaa tgacagtcag caatgtgtta cataatttgt tttttgcttt
     8041 ggcaggaaat tatattggtg gcggtttagt aatgggactg ggatatgctt ggctgaatca
     8101 gtcaaaatca tcctatgtgg actaaaaagt cgttcattaa aggggaagtg ccatggaagt
     8161 tttagttttt ccaaaaaatg aggaaaaagc gttacaaatg gaaaagtaca tgaaaaatct
     8221 tttttccttt gctggagtac ctaagccgga acgcgctctt ttacaacaac cgttactgga
     8281 tgagagcaaa aatttatcta tttctgaagt tttagctttt gttcattatt attatgaaca
     8341 aatcgaaaga gagtatcagt atgtagcgat cgatttagtc cagaaaaaca tcaaacggct
     8401 tacatttgaa gacttagtcg tattgttgcc actagttcag aaaaaagaat ggtgggacag
     8461 catcgactct tggagaaaag tatacagcac atggtgtaaa gcctacccag agcagcgtga
     8521 agaagtcttt caattgtttg aaaaaaagga agatttttgg ctacgtcgaa tagcaatcac
     8581 gttgcaatta ggttataaac agcaaacaga cacaggacta ttgatccgag caatagacga
     8641 agatcgtcaa acaaaggaat tctttatcca aaaagcaatt ggttgggcat tacgcgatta
     8701 tagtaagacc gatgccgatt gggtaaaaag acagctgaca acagatttga gtaagttagc
     8761 tgttcgagag gcaagtaagt atttataaga aagcgagacg acaagagatg agacgcaaat
     8821 tatttgcttt tgatattgat ggaacgttgt tagattcaaa caaacaagct ttagatagca
     8881 caagagaagc attggaaaaa ttacgcaaag ccggtcatct ggtcacgatt gctacagggc
     8941 ggagccgatt tcatgcgcaa gagatcattc gcgatcttga ttttacgaac tacattttat
     9001 gtaatggagc ggccggtttt ttagaccatg aacaagtata taagaattta ctagatcgcg
     9061 accagttgga tgcgttcgtt caagaagcgc atcatgagca gatcgataca gcttttgtca
     9121 gcttagacaa tatcaagcgt tcttcttcca atgatgtaac ggtgatggaa gaagcgatgc
     9181 attctttcgg tgctgtcttg cctgaattag atcagttttt tgcagaggaa caagaaatat
     9241 accaagcgtt agcatttttc gatcaaacat atgatgggaa gttctcttca tatgataaaa
     9301 ttcgatttgt ccgctggcat gagaacagtg tagatgtcgt acctaaaaat ggttctaaag
     9361 cagcgacaat tctgcactta gcagaacaag taggcatccc tcatgaagac atcatcagct
     9421 ttggcgatgg acaaaacgac cgtgaaatgt tgcgcatgtc aggaattggg atagctatgg
     9481 ggaatgctgt gccagaagta caagcagaag ctaagatggt cactgataca aacgatcagg
     9541 atggaatttg gaaggcattg aaagagttaa gcgtgattta aaagaggttg tgaaaaagct
     9601 gttctgcatc aagtaataag cccagccaaa caaaaatagc tcctcctatt tttcgttttt
     9661 ctgggcttat taccgcagat gcaagctttc gaacaccgtt taaggaagag gtcgtgacag
     9721 aagtggtcag tttcaagaaa taagaaggga tttacgaaaa ttgcttctca aatttttgta
     9781 aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga aatgggactg
     9841 tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact aagaaatacc
     9901 aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta taatatagag
     9961 tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg atgtagtagg
    10021 ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga agtaccgtcc
    10081 gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca cagaaaaaat
    10141 ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc atacgtggga
    10201 caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc cggcaaattt
    10261 tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag tcgaactggc
    10321 agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt ttcgtaaaat
    10381 gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag attttcatgg
    10441 agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag tttcagcagt
    10501 ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa gaggaacagc
    10561 gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta tgcgcagaga
    10621 acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg tggaacaagg
    10681 ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc atgcaaaaga
    10741 aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag agaggcagtt
    10801 ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa cttttaagag
    10861 agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa aaccagtatt
    10921 tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat tttgcccact
    10981 atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc atgcaagaat
    11041 ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca gatgatctgc
    11101 ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag gaggaaaaac
    11161 atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat caaggcccaa
    11221 taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt ttacgaagat
    11281 gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg aaatgcagaa
    11341 gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat cgatgccttg
    11401 attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa gacgactaaa
    11461 gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat ggacagcaaa
    11521 ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg aaatgaattt
    11581 ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt gcatgatgaa
    11641 gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt aggcagtgag
    11701 atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt ttctcaacaa
    11761 aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt cgatccgctg
    11821 gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa gcggagttta
    11881 gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat ggattattat
    11941 tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca cggcactctt
    12001 ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa acaatggcta
    12061 gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt cgcttctttg
    12121 ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt ctatgatctg
    12181 gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt gatccagctg
    12241 aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat cgatcaaggt
    12301 caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca attgatcgac
    12361 caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat caaagatgga
    12421 ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa atggttggca
    12481 gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat cggatttaac
    12541 cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc agaactggag
    12601 aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc agaactaaaa
    12661 gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga atatcagttg
    12721 tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt acttgcaaaa
    12781 gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg ctatcagtat
    12841 gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg acgacatcct
    12901 gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga aatggcagaa
    12961 gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac gtatatgcga
    13021 cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc aaaacaagca
    13081 gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga cttaattgct
    13141 ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg ttatgctacg
    13201 ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta tgacggaatg
    13261 gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa aacactcttt
    13321 tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct taaaaatgtc
    13381 catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat gatggatgga
    13441 cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc ttccaatcta
    13501 ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg acaccatact
    13561 gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat aaaaccacct
    13621 gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa ccaacagctt
    13681 tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact gaagcaaata
    13741 aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt acaaaaacaa
    13801 ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa cgtttagcta
    13861 atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa gaattagtgg
    13921 aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa gcgggactgc
    13981 ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta gagaatgcct
    14041 tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc atacgaacgc
    14101 tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg actctggaaa
    14161 cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga gaagttattg
    14221 agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat ctgtttttca
    14281 atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg aacatcgggg
    14341 acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta gtacatgatg
    14401 gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt gcgggaatct
    14461 acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta gattttgagt
    14521 tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac ttatcgacga
    14581 tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt gcaggttatg
    14641 ggtcaaaatt aatggtcggt cgtttcccta ttgctgtatt ggaaatcaaa atggacccgc
    14701 ttttagtaga tgtaaatgtt catccgacga agcaggaagt ccggctgtcg aaggaaaaag
    14761 aactaacaga gctaattagt aacgccattc aaaaagcatt gtctcaagaa aatctgattc
    14821 caagtgcagc ggataatcta cggtttaaga aaaaagtacc tgatcagcct aaaacagaac
    14881 aaatcgaaat cgatttgtcc tacgcttttg aagaagaaaa acctaaaaag ccaagtagtt
    14941 tgaattttga tcgagagaca ggtacctttt tcgtagaaga actaaagaac acatttgttg
    15001 acagaattgt tgataagtct gtggaaaatg ttgaaaacta ttctggtttt tctgaagaaa
    15061 acaccactta tccagaaaaa acagcgaaaa cggtggataa gtttggtgaa aatgttgata
    15121 actttgtaga aaaaacagtg gaaaaatctg aaaagaaaga atttcccatt gtggataaca
    15181 tggagaaaac aaatgaagaa caagcagata aatccgtaga agaggatgaa aagatttacg
    15241 aggaagaatt gacactccat cctgaatttg atttcactca aggaaacgac aaaacattag
    15301 aacaagccat caagaaatta gaaacggaaa aaccagtaca acgttttcct cagttggaat
    15361 attttggaca gatgcacgga acgtatcttt ttgcccaaag tagcgatggg ttgtacatca
    15421 ttgatcagca tgctgcccaa gagcgcatta agtatgaata tttccgggaa aaaattgggg
    15481 aagtatctga tgatctgcaa gaattgcttg ttccatttgt gttagactat ccaaacagtg
    15541 atgctttgaa attaaaagag caaaaacact tgctagaaga tgcagggatt tatttagaag
    15601 aatttggtca aaacagcttt attgtacggg cccatcctac ttggtatccc aatggagaag
    15661 aagaatcgat tattcgggag atgatcgata tgctgctgac aactggaagt gtgagtgtaa
    15721 aaaaattccg tgaagcaaca gcaatcatga tgagttgcaa acgatcgatt aaagccaacc
    15781 attatttgaa tgaagcacaa gcacgtgtgc tgctgaagga tctggcacga tgcgaaaatc
    15841 ctttcaattg tccccatggc cgtccagtat tgattcactt taccaattcg gatatggaaa
    15901 ggatgttcaa acgaatccaa gacccgcatt aagtcaaaga gaagagggaa aacatgaaga
    15961 ttgtattagc atcccaatca ccaagaagaa aagagttatt ggctcatatc gtttcagaat
    16021 tcgagattga acctgctgat atcgatgaaa cgccatttcc agacgaacag cccgtcgatt
    16081 atgttcgtag aatggctgaa gaaaaagcac gaactgtctg ggaacaagat gaaaaagaag
    16141 accatttagt gattgccagt gacacgactg ttgttctcaa tcaagagatc atgggtaaac
    16201 cagaagatct ggctgaggct gaattcatgc tgaaaaaact aagcggggaa acacatactg
    16261 tatatacagc tgttgtttta aaaacaaagg aacgagaaga acggatacta gcagaagcac
    16321 atgtcacttt ttatcctttg acagacgaag agatcaaacg ctacttggag acgggggatt
    16381 atgcggataa ggcaggggca tacggcatcc aaaatgctgc gagtgtattc gtcaaagaaa
    16441 tttctggaga ttattacagt atcgtaggat ttccgattgg tgctgtcaat caggcactga
    16501 aaacattcat ttaaacaaaa aagcggacta tctgattttc gattgaaaat ccatgatagc
    16561 ccgttttttt tattgaaaag attacttatc tactctgact aagattttac ttcagcttta
    16621 ccgactgctt cgcccgggcg aacaagtcct gattggtcaa gagaaagtcc tgcaactttt
    16681 tcattattgg tcaaagcaac aataatgtca gttttcttgc cagcttgttc tatttctggc
    16741 aaatccattt cagcaatcag ggtctctggt gtgacaaggt atccttcttt cacgaataca
    16801 ttgaaaggaa caccctttag ttcaacagta tctaatccca tgtgaacaag tacttccagc
    16861 ccctcattag aaagaatacc aatggcatgt tttgtttcaa agacactcgt cactttaccg
    16921 tttactggtg catagacttt gccgtttgac ggttcgacag cataaccgtc acccatcatt
    16981 ttttgtgaga aaactgggtc atttacttca gttatttcct tgatggtccc tgcagcgact
    17041 gcgaataatg tttcttttgt tgcaactgcc gtaccagttg ctgaaacagg ttcgaaagac
    17101 tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta catcagcagc attttcagga
    17161 acagcaggat caatcttatt caaaattccc tgacgtctaa agatgatcgt caagccaaat
    17221 ggaatgatga cagcaatgat cattgcgatg atgaaaggta cccatgtttc tgcttggata
    17281 gctagtattc ctggcaaacc gcctactccg atcgcgttag ctcgaacacc catcaagtta
    17341 gcaaacatgc cagctaatcc agagccgacc attgcagcga cgaaaggata gacgtatttc
    17401 aaattgattc cgaacatagc aggttcagtt acgcctaagt aacaagaaat cattgctgga
    17461 atcgagattt gttcttcttt tttattccca cgatgtaaga agatgatggc taagactgca
    17521 gagccttggg cgatatttga taaagcaatc atcggccata aattcgttga tccgaaatct
    17581 gcaatcagct gcatatcgat ggcattggtc atatggtgga gaccagtaat cactaaagga
    17641 gcatacagga atccgaatac ggcactaaac aaccagctga tagaagacgt caatccagta
    17701 tttacgatat ttgagatcca gcttccgact gtccagccga taggacctaa aataacgtgt
    17761 gcagctaaaa cagttggtaa caatgaaaac aatggaacaa aaatcatcga gatcgactga
    17821 gggatatatt tgcggaagaa gatttctaga tacgctaaca taaaccctgc cagcatggct
    17881 ggaataactt gcgcttgata accaatcatc tggacttgag caaaaccaaa atcccaaaat
    17941 ggaatatctg ctgcggctgt ggaagctaca ctataagcat ttaataattg cggagaaact
    18001 aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac ccatcttttt tgcaatactc
    18061 caagtgatac ctactggaag aaaatggaaa attgcttcac ccggtaacca caaaaaggca
    18121 ttgacaccat tccaaaactt cgagtgttca acgattgttc caccaagact ttcaaactgg
    18181 ataccttcaa gtacatttct gaatccaagg atcaatccac cgacaatgat tgcaggaatc
    18241 agtggtgtaa agatttctgc taaaacggca atagcacgtt gaacgggatg aaggttttgt
    18301 tttgcagctg cttttccttg ttctttggaa accccttcaa ctccagatac tgcaacgaag
    18361 tcgttataaa aagtagaaac atcgtttcct atgatgactt ggaactgtcc tgcattcgtg
    18421 aacatccctt ttacgctagg tatatcttcg atcgcttttt cgtccgcttt tcctggatca
    18481 ttcaggacaa aacgcatacg agtcgcacaa tgactaactg cagcaatatt ttcttttccg
    18541 ccgatatcat gtaacagctt ctcggcatct gcttgatatt ttgccatatc aataaacccc
    18601 tttctcttaa cttgtctatg caagttgttt cttcatttac tatacttttt ttcggagaga
    18661 attgcaagcg ttttattaaa aagtacgaga gaaaatgata tcgttttaaa aagcttaaaa
    18721 taagcttttc ttaaaaatag gaacattgaa atacaaaaag agctgtagta tagtgaagaa
    18781 atgatggaag aacgagtgag ggaaaaaaca tgaataagtt caatgaaatt tttttagatt
    18841 tggaacagaa aattttagcg aaagaatatc ctccgcattc gcttttaccc agtgagaacc
    18901 aattgatcaa aatttatggt gtttcacgag aaacaattag aaaagcgctg aatcttttga
    18961 ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc gatcgtactg gatcggaatc
    19021 gcttcgactt tcctatttct ggattgacta gctataaaga gctacaggag acccaacgta
    19081 ttccaagtga aacgatcatc catacgttag aagaaacaga agtgacaaaa gcattgaacg
    19141 agatcactgg ctgggagatc ggtgcgcctg tttggcattt gatacgcgaa agaaaaatca
    19201 atggcgaagt agttatatta gacaccgatt atctattaaa ggaaatcgtt cctattttaa
    19261 ctccagcaca agcacaagga tcgatctatg aatattttga aaatgagctt tctttgacga
    19321 ttgcttacgc gcaaaaggag atcacagtag aagaagtaac ggaaatcatg aaaacagcga
    19381 tggatcttca cgatgacact tatgcagtag tagtcagaag cctcgttcat ctggaagata
    19441 cccgctgttt cgagtattct gagtcggttc accgattaga taaattccgt ttcgttgagt
    19501 ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc aaattgttgg catctgtcca
    19561 ctttggcgct atgataggat cacaaagggg gcatttttaa tggataaacc aataagacca
    19621 gaaaaagaag aactgaaaga aaagttatca cccatggctt atgcagtcac acaagaaaat
    19681 gcaacagaac gaccatttag cgggaaatat gatgattttt acgaaaaggg gatctatgta
    19741 gatatcgtca gcggtgaacc gttattcagc tctgcagaaa aatatgatgc aggttgtggc
    19801 tggccatcat tcagcaaacc aataacgaaa agacaagtgc gcgaaaaagc cgatttttct
    19861 catggcatgc atcgaataga agttcgaagc aaagaagcag attctcatct cggacatgtg
    19921 tttaccgatg gaccagtaga tcaaggtggt ctacgttact gcatcaattc tgcctcactg
    19981 aaattcattc catatgaaaa gatggatgaa ttaggttatg gggaattcaa atcacttgta
    20041 gaataatgga aatacacaaa gagcggtaca atacggggat ttactcgatc gtccgctctt
    20101 tttttattca agcgattgct atttgaaaga aacgctccct agactccttt tttcacaagg
    20161 tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag aaagaggact agaatatgta
    20221 tgaatatctg acaggtgtgg ttacctttat caacccctat tacatagtag tagaaaccaa
    20281 tggtatcggc taccagatcg cactaggaaa tccttaccgc tacagtagca aactaaatca
    20341 aaaaataaaa atttatgtcc atcaggtaat acgtgaagat gcgcatcttc tgtatggatt
    20401 tgattcgcta gaagaaaagc aactgttttt gcgattagta agtgtttcag gaatcggtcc
    20461 gaaaagtgcc cttgcaatca tggcaagcga agaccactcc ggtttgattc aagcagtaga
    20521 atccagtgat gtcacttatt tgacgaaatt tccaggagta gggaaaaaga cagcacaaca
    20581 aatgatcttg gatctaaaag gaaaattcgg tgagctgagt atcgatacac cttttagtct
    20641 atttgatgaa gcaaacacaa aagacgctac agctctatct gaagcaatag aagctctctc
    20701 ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa aaacaattaa aagaagtaga
    20761 taatcagaca acggatgagt atctacgcca agcactcaag ctaatgatga aaaaataagg
    20821 aggagaccaa atgacagatg aatacttgtt atcaccagaa tctggcgaaa atgaactgag
    20881 tttggaaaaa tctcttcgac cacagctgct ttctcaatat atcggacaga gaaaagtcaa
    20941 acaagaactt cagatttaca tcgaagctgc cagaaatcgg gaagaacctt tagatcatgt
    21001 tctgttgtac gggcctcctg gattaggaaa aacgacgatg gccatggtca ttgctaatga
    21061 gatgaccgta aatattcgta cgacgagtgg ccctgctatc gaacgtccgg gtgacttagt
    21121 agcaatcttg aatgaactgg aagcagggga tgtcctgttt atcgatgaga tccatcgatt
    21181 acctagagtg gtagaagaga tgctttattc agctatggaa gatttctatg tagatatcat
    21241 ggtaggacaa gggccaaccg cacatccggt tcatttccct cttcctccct ttactttgat
    21301 tggcgcgacg acacgggcag gtatgctttc tgcaccttta cgagatcgat ttggcatcat
    21361 ttctcatatg gaatattatg acgaacagga tctgcaggag atcgttttac gttcttcgga
    21421 tatttttcaa acagagatca ttgaagaagg ggcattggaa attgctcgcc gatcacgagg
    21481 aacgcctaga atcgctaatc gcttgctcaa acgggttcgt gattttgcgc aagtccaagg
    21541 agacgggaaa atcgatcgtc ctatagcgga tcaggcactt actttgctac aggtcgatca
    21601 ggctggattg gattatgttg accagaaatt gttgcgaaca atgatcgagt tatatggcgg
    21661 cggaccagta gggttaagta cgctttcagt aaacatagga gaagagcgtg agacggtaga
    21721 agatatgtat gaaccgtatc tgatccaaaa aggattttta aagcgtacac ctagagggcg
    21781 aattgctact gcttatgcct atgaacattt tggttacgac tatcatatat gatttttgct
    21841 tttagattgg aggaaaatga atgagtgata caacacgtac gaaacaattg atcaaaaacg
    21901 tattgattga tttgtgccaa acgcaatctt ttcgaaaaat cagcgtccaa aaaatcgcaa
    21961 acgaagcaaa aatcaatcga caaacatttt attatcattt tacagataaa tacgatttgc
    22021 ttcgttgggt ctattgtgag gatgcattgc attatttaga tccacaatgt ctttcacttg
    22081 ataattggga agagcaagca ttgaaaatgc tgaaagcaat tgctgagaag aaagaatttt
    22141 atgcaaatac tgtgctttct gacccagaaa ttttacaaaa agagttttct caactgatcc
    22201 aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga aagacaactt tctgcaagag
    22261 acaaactatt ttatgctcgt tttttttctt acggttgcag tggtgtatta gtcaattgga
    22321 tcacgaatga atatgaagaa acaccgatgg aaatcgctat gcagcttttc cgtttagcca
    22381 aggatacgga attatattct tatcgtcttt atgcaaaaga agagttggaa gaacagtaat
    22441 agaaaaattt tttgttggaa aagcaataat cgctaaaagg agatggggaa atgacaaaaa
    22501 ttttatttgt ttgtttggga aatatttgcc gttcaccgat ggcagaaggc ttgctgagaa
    22561 aacgaatagc attagaagga agggaagcag aattttttgt ggattctgct gctacaagca
    22621 cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa aatattaaac caagaaaaag
    22681 tagatatgac gaatatggtt gctcggcaaa tcacacccca cgattttgaa acttttgatt
    22741 ggatcattgg catggatcaa gaaaatgtag aagaactgaa acgaagagca ccacgctcag
    22801 cgcaggggaa aatccatttg tttttaagta gcattcctgg aaaagaaaaa gaaaatgttc
    22861 ccgacccata ctataccaat aactttgatg agacatatca cttgatcaca gaaggcctga
    22921 atcattggtg gaatatttgg attgatgaaa ataaaataac aaataatttg taaagttaac
    22981 gctttctttt taaggcgatg ttagagcatt tccttgtttt tttgtaaaat gttaggtaaa
    23041 ataaaattaa ttaagtaaca ctttcttagc taaaggagga gtgcattcga tgaacgaaga
    23101 gacagtatcg ccaatgaaga agcttacgaa gttagttact catacttcgt atcaactttt
    23161 tgattctttt gtaaatgaca ctgaagaaca tgctaagaat gtacgattgc aaggaaagtt
    23221 tattcttgag ctgcaaaaag ctgctactga acatagtcta gttatcttac aagtaagagc
    23281 aaatcgtttg agtagcaagt atgaaacgat ctcaggctgg gtggcgacaa aaacggtcga
    23341 aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag cagatccgga tggttcctgt
    23401 agaacaaatt gaaaaaataa caatcctttc accagcaggg gaacaggaac ggttatcaaa
    23461 ataatttata ccagatagaa aagcacgtat gagcttgtac catcaatcat tactaatcaa
    23521 aaataaccga atgctttaag ctgatatcag taaaacggaa cgttttagga tgagtatcat
    23581 agcatgacag cattcggttg attagttgaa tcgattggat ggtacaagtt tttttgataa
    23641 aaaaaggtcc tgctagccgg ggagctagca ggataaggag ttaaaaatga aaaagtgttg
    23701 ttagggttgt ttgttggtat gcttatataa tagtcattag ttgtgaatat tttgtgacga
    23761 aagttttcga aagttgttaa gtt
//
LOCUS       DACECB010000047        23571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECB010000047 DACECB010000000
VERSION     DACECB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.047"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO04_001849"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO04_001849"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0213451.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO04_001850"
     CDS             complement(1172..1867)
                     /locus_tag="IWO04_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0213452.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO04_001851"
     CDS             complement(1880..2596)
                     /locus_tag="IWO04_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0213453.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IWO04_001852"
     CDS             2926..3531
                     /locus_tag="IWO04_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0213454.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IWO04_001853"
     CDS             3856..4179
                     /locus_tag="IWO04_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0213455.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IWO04_001854"
     CDS             4361..5731
                     /locus_tag="IWO04_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0213456.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IWO04_001855"
     CDS             complement(5861..7162)
                     /locus_tag="IWO04_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0213457.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IWO04_001856"
     CDS             complement(7392..9383)
                     /locus_tag="IWO04_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0213458.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IWO04_001857"
     CDS             complement(9527..9793)
                     /locus_tag="IWO04_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0213459.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWO04_001858"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWO04_001858"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0213460.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWO04_001859"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWO04_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0213461.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IWO04_001860"
     CDS             13137..13700
                     /locus_tag="IWO04_001860"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0213462.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IWO04_001861"
     CDS             complement(13953..14474)
                     /locus_tag="IWO04_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0213463.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IWO04_001862"
     CDS             complement(14477..15319)
                     /locus_tag="IWO04_001862"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0213464.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IWO04_001863"
     CDS             complement(15312..16037)
                     /locus_tag="IWO04_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0213465.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IWO04_001864"
     CDS             16213..16509
                     /locus_tag="IWO04_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213466.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IWO04_001865"
     CDS             17077..18669
                     /locus_tag="IWO04_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0213467.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IWO04_001866"
     CDS             18682..19422
                     /locus_tag="IWO04_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213468.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IWO04_001867"
     CDS             19590..20939
                     /locus_tag="IWO04_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0213469.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IWO04_001868"
     CDS             complement(21067..21657)
                     /locus_tag="IWO04_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0213470.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IWO04_001869"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IWO04_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0213471.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACECB010000048        22379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.048, whole genome shotgun sequence.
ACCESSION   DACECB010000048 DACECB010000000
VERSION     DACECB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.048"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..986
                     /locus_tag="IWO04_001870"
     CDS             123..986
                     /locus_tag="IWO04_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0213472.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1115..2737)
                     /locus_tag="IWO04_001871"
     CDS             complement(1115..2737)
                     /locus_tag="IWO04_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0213473.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2986..3507
                     /locus_tag="IWO04_001872"
     CDS             2986..3507
                     /locus_tag="IWO04_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213474.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3693..5423)
                     /gene="spxB"
                     /locus_tag="IWO04_001873"
     CDS             complement(3693..5423)
                     /gene="spxB"
                     /locus_tag="IWO04_001873"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0213475.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5588..6406
                     /locus_tag="IWO04_001874"
     CDS             5588..6406
                     /locus_tag="IWO04_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0213476.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6509..6706)
                     /locus_tag="IWO04_001875"
     CDS             complement(6509..6706)
                     /locus_tag="IWO04_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213477.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6875..7453
                     /locus_tag="IWO04_001876"
     CDS             6875..7453
                     /locus_tag="IWO04_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0213478.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8498..9001)
                     /locus_tag="IWO04_001877"
     CDS             complement(8498..9001)
                     /locus_tag="IWO04_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0213479.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9360..10385
                     /locus_tag="IWO04_001878"
     CDS             9360..10385
                     /locus_tag="IWO04_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0213480.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10708..11586
                     /locus_tag="IWO04_001879"
     CDS             10708..11586
                     /locus_tag="IWO04_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213481.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12062..12178
                     /locus_tag="IWO04_001880"
     CDS             12062..12178
                     /locus_tag="IWO04_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0213482.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13106..13444
                     /locus_tag="IWO04_001881"
     CDS             13106..13444
                     /locus_tag="IWO04_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213483.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13538..13768
                     /locus_tag="IWO04_001882"
     CDS             13538..13768
                     /locus_tag="IWO04_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213484.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14168..14539
                     /locus_tag="IWO04_001883"
     CDS             14168..14539
                     /locus_tag="IWO04_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0213485.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14813..15505)
                     /locus_tag="IWO04_001884"
     CDS             complement(14813..15505)
                     /locus_tag="IWO04_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0213486.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15508..16293)
                     /locus_tag="IWO04_001885"
     CDS             complement(15508..16293)
                     /locus_tag="IWO04_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0213487.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16530..17930
                     /gene="lacG"
                     /locus_tag="IWO04_001886"
     CDS             16530..17930
                     /gene="lacG"
                     /locus_tag="IWO04_001886"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0213488.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17955..18272
                     /locus_tag="IWO04_001887"
     CDS             17955..18272
                     /locus_tag="IWO04_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0213489.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18285..19958
                     /locus_tag="IWO04_001888"
     CDS             18285..19958
                     /locus_tag="IWO04_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0213490.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19972..21603
                     /locus_tag="IWO04_001889"
     CDS             19972..21603
                     /locus_tag="IWO04_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0213491.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         6993 a         3743 c         4507 g         7136 t
ORIGIN      
        1 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
       61 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
      121 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
      181 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
      241 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
      301 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
      361 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
      421 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
      481 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
      541 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
      601 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
      661 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
      721 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
      781 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
      841 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
      901 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
      961 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
     1021 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
     1081 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
     1141 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
     1201 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
     1261 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
     1321 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
     1381 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
     1441 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
     1501 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
     1561 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
     1621 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
     1681 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
     1741 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
     1801 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
     1861 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
     1921 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
     1981 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
     2041 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
     2101 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
     2161 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
     2221 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
     2281 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
     2341 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
     2401 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
     2461 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
     2521 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
     2581 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
     2641 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
     2701 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
     2761 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
     2821 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
     2881 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
     2941 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
     3001 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
     3061 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
     3121 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
     3181 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
     3241 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
     3301 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
     3361 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
     3421 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
     3481 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
     3541 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
     3601 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
     3661 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
     3721 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
     3781 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
     3841 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
     3901 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
     3961 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
     4021 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
     4081 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
     4141 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
     4201 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
     4261 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
     4321 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
     4381 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
     4441 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
     4501 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
     4561 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
     4621 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
     4681 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
     4741 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
     4801 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
     4861 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
     4921 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
     4981 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
     5041 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
     5101 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
     5161 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
     5221 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
     5281 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
     5341 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
     5401 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
     5461 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
     5521 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
     5581 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
     5641 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
     5701 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
     5761 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
     5821 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
     5881 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
     5941 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
     6001 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
     6061 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
     6121 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
     6181 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
     6241 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
     6301 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
     6361 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
     6421 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
     6481 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
     6541 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
     6601 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
     6661 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
     6721 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
     6781 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
     6841 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
     6901 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
     6961 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
     7021 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
     7081 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
     7141 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
     7201 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
     7261 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
     7321 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
     7381 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
     7441 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
     7501 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
     7561 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
     7621 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
     7681 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
     7741 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
     7801 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
     7861 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
     7921 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
     7981 tatctcattt tacgcttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
     8041 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
     8101 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
     8161 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
     8221 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
     8281 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
     8341 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
     8401 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
     8461 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
     8521 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
     8581 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
     8641 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
     8701 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
     8761 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
     8821 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
     8881 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
     8941 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
     9001 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
     9061 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg
     9121 aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt
     9181 tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt
     9241 ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac
     9301 gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat
     9361 tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc
     9421 tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt
     9481 caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat
     9541 atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga
     9601 tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca
     9661 gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc
     9721 tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt
     9781 atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt
     9841 cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt
     9901 tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc
     9961 acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag
    10021 cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg
    10081 ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc
    10141 tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta
    10201 atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga
    10261 tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg
    10321 ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag
    10381 aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct
    10441 aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg
    10501 aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    10561 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    10621 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    10681 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    10741 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    10801 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    10861 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    10921 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    10981 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    11041 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    11101 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    11161 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    11221 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    11281 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    11341 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    11401 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    11461 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    11521 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    11581 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    11641 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    11701 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    11761 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    11821 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    11881 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    11941 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    12001 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    12061 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    12121 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    12181 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    12241 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    12301 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    12361 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    12421 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    12481 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    12541 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    12601 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    12661 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    12721 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    12781 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    12841 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    12901 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    12961 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    13021 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    13081 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    13141 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    13201 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    13261 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    13321 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    13381 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    13441 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    13501 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    13561 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    13621 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    13681 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    13741 tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat
    13801 attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata
    13861 aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc
    13921 tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga
    13981 ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag
    14041 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
    14101 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
    14161 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
    14221 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
    14281 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
    14341 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
    14401 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
    14461 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
    14521 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
    14581 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
    14641 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
    14701 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
    14761 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
    14821 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
    14881 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
    14941 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
    15001 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
    15061 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
    15121 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
    15181 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
    15241 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
    15301 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
    15361 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
    15421 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
    15481 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
    15541 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
    15601 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
    15661 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
    15721 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
    15781 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
    15841 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
    15901 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
    15961 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
    16021 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
    16081 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
    16141 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
    16201 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
    16261 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
    16321 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
    16381 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
    16441 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
    16501 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
    16561 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
    16621 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
    16681 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
    16741 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
    16801 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
    16861 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
    16921 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
    16981 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
    17041 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
    17101 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
    17161 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
    17221 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
    17281 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
    17341 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
    17401 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
    17461 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
    17521 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
    17581 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
    17641 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
    17701 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
    17761 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
    17821 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
    17881 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
    17941 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
    18001 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
    18061 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
    18121 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
    18181 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
    18241 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
    18301 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
    18361 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
    18421 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
    18481 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
    18541 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
    18601 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
    18661 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
    18721 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
    18781 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
    18841 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
    18901 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
    18961 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
    19021 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
    19081 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
    19141 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
    19201 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
    19261 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
    19321 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
    19381 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
    19441 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
    19501 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
    19561 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
    19621 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
    19681 ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
    19741 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
    19801 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
    19861 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
    19921 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
    19981 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
    20041 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
    20101 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
    20161 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
    20221 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
    20281 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
    20341 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
    20401 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
    20461 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
    20521 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
    20581 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
    20641 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
    20701 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
    20761 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
    20821 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
    20881 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
    20941 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
    21001 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
    21061 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
    21121 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
    21181 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
    21241 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
    21301 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
    21361 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
    21421 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
    21481 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
    21541 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
    21601 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
    21661 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
    21721 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
    21781 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
    21841 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
    21901 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
    21961 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
    22021 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
    22081 ttatgttggc tctttattcc cactctgttt tcaacgtaaa gtgtataaaa cagaatgcga
    22141 tactatcaag ggagttaaaa caatatacag aacagtaaaa cactataaat aaaaataaaa
    22201 gtgcaataaa agtgcaattt ttgcacctaa tcatactctg tttatatatg aaaactagat
    22261 tctgaatcca atatttggtt aacgagaggc gttcgaattt gaacgtcttt tatatattac
    22321 tttcaaataa atatgaggta gaatactatc tattttcttt ttaacccaat tgttataat
//
LOCUS       DACECB010000049        21956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.049, whole genome shotgun sequence.
ACCESSION   DACECB010000049 DACECB010000000
VERSION     DACECB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.049"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..876)
                     /locus_tag="IWO04_001890"
     CDS             complement(<1..876)
                     /locus_tag="IWO04_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0213492.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLL"
     gene            1002..1667
                     /locus_tag="IWO04_001891"
     CDS             1002..1667
                     /locus_tag="IWO04_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0213493.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(3602..6751)
                     /locus_tag="IWO04_001892"
     CDS             complement(3602..6751)
                     /locus_tag="IWO04_001892"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0213494.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(6764..7963)
                     /locus_tag="IWO04_001893"
     CDS             complement(6764..7963)
                     /locus_tag="IWO04_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0213495.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(7953..9548)
                     /locus_tag="IWO04_001894"
     CDS             complement(7953..9548)
                     /locus_tag="IWO04_001894"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0213496.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(10132..10839)
                     /locus_tag="IWO04_001895"
     CDS             complement(10132..10839)
                     /locus_tag="IWO04_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213497.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(10904..11740)
                     /locus_tag="IWO04_001896"
     CDS             complement(10904..11740)
                     /locus_tag="IWO04_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0213498.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(11758..13167)
                     /locus_tag="IWO04_001897"
     CDS             complement(11758..13167)
                     /locus_tag="IWO04_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0213499.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(13181..15004)
                     /locus_tag="IWO04_001898"
     CDS             complement(13181..15004)
                     /locus_tag="IWO04_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0213500.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(15847..16173)
                     /locus_tag="IWO04_001899"
     CDS             complement(15847..16173)
                     /locus_tag="IWO04_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213501.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(16219..16581)
                     /locus_tag="IWO04_001900"
     CDS             complement(16219..16581)
                     /locus_tag="IWO04_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0213502.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(16618..16833)
                     /locus_tag="IWO04_001901"
     CDS             complement(16618..16833)
                     /locus_tag="IWO04_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213503.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            16960..17295
                     /locus_tag="IWO04_001902"
     CDS             16960..17295
                     /locus_tag="IWO04_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213504.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(17371..19368)
                     /locus_tag="IWO04_001903"
     CDS             complement(17371..19368)
                     /locus_tag="IWO04_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213505.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(19466..19741)
                     /locus_tag="IWO04_001904"
     CDS             complement(19466..19741)
                     /locus_tag="IWO04_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0213506.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            19973..20707
                     /locus_tag="IWO04_001905"
     CDS             19973..20707
                     /locus_tag="IWO04_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213507.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            20746..21888
                     /locus_tag="IWO04_001906"
     CDS             20746..21888
                     /locus_tag="IWO04_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0213508.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         6916 a         4340 c         3405 g         7295 t
ORIGIN      
        1 taataaaaga gaaatcaaag agataccata tattccgctg aacaagaaaa cgtacatctg
       61 gaacaaataa agaaattgct ccttataata cataaaaata aaaccaccaa tgattcctgc
      121 aattatgaat gtaataatga ttttcttttt ccccaataaa caccacaata cttgatgtaa
      181 tgaaaagtca ttattcaaca ataaactgat ttctttattc atcaataaga aaaaggatga
      241 taacaaaagg aataaaagaa tcaatccaat agttaaatac actcgataat tatatacgtc
      301 tgtatttgaa ataaccgaat acgaatagct attttcaaag tcttcaaaaa tattagtggc
      361 ataatcagtg tgaaatgctt gattgaatcg atcagtgaat agtgaaatga cccgttcata
      421 ggcttctttt tctctcgtca tcaagaaaaa ttcaccttca aaatctttta ttttgatcga
      481 ttcttcaatg gacgttacgc gaaaggaggt attagaatag ggtaccttct cgtgagaaga
      541 tttgacatcc ccttgataat acaatgtcat ttcaagcttt ggctgtatct tgaagttcca
      601 agatgtatct acttcattgg attcatttac tgtttttttc atataagaaa gctcagtttc
      661 tgatgagaca ttttcaaata ccatgattag ctgctcatat tcttcttgcc caataaaatg
      721 ttgtggcaat cgaatcggag tataagtatc tgtttgatgg aaaaattcat ttgtccgata
      781 gagtacctgt ttatttatac taaccaaggc aaaaccactc atgataccgg tcactatgat
      841 tgtgagtaaa actgtgaact tttttttgat tatcaccact ctcccttact taaatgattc
      901 tttgataaaa tcataccgtt tttcatcaaa aaaacgtatc tatcaagtgt ataggtcatg
      961 tagaataaat ctatatactg tagaatcgaa aggagaagaa gatgaacacc ggagaaaaaa
     1021 tacgaactta ccgcagaaat tgtaagctta cgcaaaaaga attaggcgaa aaaattggtg
     1081 tttccgataa aacgatttct agttgggaaa attctcgtac aatgccagac tttgaaatgc
     1141 tgtcactttt acatcatact ttaggtctgc cgattgattt tccttctatt gaatcagcta
     1201 ccgaatcaat agaaccaaca ttgccagatg aacctcttgt taacaagaaa tcttattcat
     1261 caaaacaacc cttctcactc aaaaagacac tatctttctt tctgctcgtc ttttgtttgg
     1321 tctatctttt tcttatcaat tatcaataca aaaatgaaac gatcgatcga ttcaatcctt
     1381 ttttgaaacc acaaatcggc tataccacac tccctgtagg atacgtggac aattccatcg
     1441 aacgttgggt atgtaatgat atctttggcc atggtacatg gctgtctttt cacgctggag
     1501 aggtagaaaa agagaaaaac catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg
     1561 ttcgcctgct cacttgggaa gccattccct cagattacca agaaatcctc agccaagact
     1621 atgacgacgc cttaaaaaga agccctcagg agccagatag gcattaagta ggagatgaac
     1681 gtagagatag aaaaaatcaa tgcacatgat cttatccaaa aaacaggggt acaaattaat
     1741 tgaaaaagcc ttgagatgaa agttcctaaa taaaaaagtt cagaatgata ggtatttctg
     1801 gcttatagta taatatttac gccacagcct cccaatgatt ctttttggaa tcataatcaa
     1861 ggatggacat tcacaaaaat attttgctca ttcgatccga atagattaac gaccaatcta
     1921 gtaagtaata tagattggta cgtagtcaag ctgcacataa tttagatcaa ttttagttct
     1981 tttttatatt tctatgatct gctcatttac ttttatatga tggcttacag taacataaat
     2041 aaatatgata atcgctggta gtttatccaa gagtactcaa taaaatacaa aatggattgt
     2101 tcaagcgcct atcataatga taaaatgaaa caaataaacg atacaaggag gaagagactt
     2161 tgagtaatgg cagaacaatt ttttcattgt aaaaattgtt cagttgttaa tctatgggat
     2221 ttcggtagca tgggcatttt tttatagagg aatcacacca acctggccgt tttatctctt
     2281 gttagtggga atattagtag gaaattttgc ccctcttcac tatcgtacga acttttttcc
     2341 aactgattct ggcctttttc ttaaaacaaa gacgcgaaaa ttagaaaaca ttattgaaat
     2401 gacaattacc tgtataatca ttttcttagt tatattccta aacgtcttct aataagaatc
     2461 tcttaaaatt agcttgttac agttagttac ctagtttata taacacagtt atcttatctt
     2521 ccttatcaac atcttttgcc aactctttgt cttaaattcg aaacatataa gcagagctac
     2581 gaacaccaag actgtttcgg ctctgttttt tgcatcaaat tcactcttat atagctgata
     2641 atctcggctc tttaaaagaa attattttcg cttcatttta ttataaaaag gctaggaacc
     2701 ttgatgttat caggttccta gcctttattt aagtattacc ccacagaccc ttccatatag
     2761 atttttaaat tacacactag tttaaactaa aacaaacaag gaaaaactta ccatttaatc
     2821 aaaaaattag ctcatatgaa tatattagaa tgtaaattag actggtcaaa atctggtcaa
     2881 aaaagaggac gaatacaggt agtcctcttt tttgttattt tgatattcta aatcattttt
     2941 tattggatta ttcccttgtt cgatatagac ccaaattgat ctatcaataa ttatcaaact
     3001 gagtcatata gagacaaaaa tttcaaaaat gacctgttaa atggtcatcg aatatcattt
     3061 aaaatcaata cgacggagtc agacacggag tcaaaacaag cccgctgaga gtaaaaaata
     3121 tacagtccca taggtaaagt gccacaggac tgtatatttt tttatttgag attacctgaa
     3181 atgattccat ttaattttta tctcattttt tcctactagt tagtatcaaa tactctccta
     3241 ttttctcaat aaactatttt gggttacatg gtatctgaaa cagctaaaac cttgtcatta
     3301 tagcaaaaaa tcactttatc tccgtttcat ttcagataca tttcacttaa gcggattcca
     3361 aaaaaacaaa agaaaaagga acccctagtt agcctatgct ttttattggt tcctctcaaa
     3421 acacggtcat ataacaacgg ggtggggtaa atgaatgaaa aaggtggagg aaacattatt
     3481 caatagctgt cttaaatact tcgttataaa atgtgaacaa ataaaagaca tctatcccta
     3541 ttttctacac ttccacttga ctaccatctt tgaatcgtac aattttctta actcttctcg
     3601 tttacctcaa ttcattctca agtggaatca actcatcatc taacatttta cgccatgctc
     3661 gcttcatttc ttgtgggtat ttaaatggct tcgcttctgg atgtttggct ttatatcctt
     3721 cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca cctggtagat
     3781 attgcagatt agaagcatgt aattctttct cagaaacaaa ccactcattg gaaaagacat
     3841 gaatcaaatc atcactcgct tgtgtaaaat agcgacgttt aatgacaaag atatcttcat
     3901 tactatctaa agtccccaca tcaaatgcat ttaaaatttg tcggtaaact tctttgactc
     3961 cttctgtttt attcaattta gccaaagctt tttcaatttc ttctcgtgca tctgaactat
     4021 tcgcagcata agttcctagt aattgatcaa tataagttga atcaatatca tacaacttag
     4081 tggtattttc actataaaat tcgatcgttg aaaaatcttc tccttctttt tctggttctt
     4141 cctctattaa tgaacccttc acggtctcgt acacgccaat ttgttcttcc aaaagttgaa
     4201 tttgattgtt caaagttgga gatttcacca tgtcatcatt gtaatcgtca taagtcacta
     4261 acgattcata agcacgattc aattcttgaa atgctttgac gtactcgata cggatttcaa
     4321 gtggcgtatg ttcatcaatg gattccatgg atgaaacctc ttttaactta ccatatgctt
     4381 gtttgaatct ttttttggat tcctgataac ttggataaat caaacctgat tcttcttttg
     4441 cttttgaata cagttttgtc gcatcctcga cattcttctc cattgtcgct ggcttacgaa
     4501 acgtcacaat caatcctttt gtcttatcag gatacgtacg attcgtacgt gaaaaagctt
     4561 ggatcaatcc tgcatactct agattacgat ccacaaatag ggtttgaatc gtaggggcgt
     4621 caaaacctgt taatagacga tccactacaa tcgctaaatc gatttgtttc ccaaattgtt
     4681 tgaattccgc tctttttcgt gccaaacgat tattgatgtc tccgttataa cgctcaatgt
     4741 ctgctaatga ccaggaagta tcataatact gattatattc ctcaataatt ttttgcattt
     4801 cttcttgttg ggcacttgaa tctcggctat tttcttctaa tgaataggta atagcgattc
     4861 gtggaaaatc aggatcctcc atcgttcgtc cttcacgaat gagctgattg ggaaactctt
     4921 ttgttaacca atcaggctct ttcgtcatct ctttgatggc ccgataatac tttttggcca
     4981 tgtcaatgga actcgtggtt aagattgcag atttcgttgg cctgccattt cgaaaatcaa
     5041 attttgtata ggcattatct ggacggaaca ttttatgaat gactttttgg atatgttcat
     5101 ctgcttcaaa aacagctggg tccaaataac tttctttttt gacaggttcc atttgatcaa
     5161 tcatacgatt gatctgttct gatgaatact ctgcatatgt ttctacttct tttaatttcc
     5221 gatagatttt attctctagt gaagtcggct ccacggtgtt ttcatgctct acttgaaatc
     5281 ctaagacaga gccatcttct agcgcattct taatggtata ggtatgcaag acctctccat
     5341 actgatcatg ggttgtacgt gccaattgcc ctttggcttg tttccgattc tcaggaaaaa
     5401 tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa catttttttg atttcttcca
     5461 tattttcagc acttaacgct cgatgacact catctactac aaacacgata tgttggccca
     5521 ttaatttttg gaagcgattg gttccttttt tctcttcttg tttctttgca tatttcaacg
     5581 ccgcatctag tttttgacgg gttgtaatga ttacaacatt tgcattggca tctgccaaca
     5641 gggtttcact taattctttg gcacttcccg ttccaacgat caagctattc gctttggcgt
     5701 tcccggaaga aatcccagta ttaaattccg aagcaaattt tgtaaactct gtggtggttt
     5761 gattatctaa atctttgcgg tccactaaca tgatcgttcg atcgatcccc gattttcgtg
     5821 ctaaaagctt agtagataca aaactagtca acgtctttcc tgaaccagtc gcatgccaca
     5881 cataccctga ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca tgtacttgat
     5941 aaggatgtaa caccatgagg gttttattat cttgatcttc actgacgatt gtataatccg
     6001 caatcaaacg atgagcatcc gggatattta acacttgttt acaaaactca tataagtttt
     6061 ctactttttc attgtctttc gttcgccaac taaataaaaa cttagggtgc aagtctttcg
     6121 gtaaggcatt tgcaaaatac cgagtggttt gttcattgga aaccacaaac aactgtaagg
     6181 tcgaaaagat attgttccga aacatacctt cttctgcata ttttttgatt tggttaaatg
     6241 cctgataaac accatctttt gcagttactt gtttcagctc gatttggaca atcggtaatc
     6301 cattgattaa taaggtgacg tcaaaacgtc gatcccgtgc ttctatgtta cttccacgtt
     6361 ttgcaatttg atggaccact tcataagtag agattcctcc accaatgtct tgattggaat
     6421 aaaggataag tgagacagaa cccaattgcg aatcttcccg ttcaatcgtg atccgagcca
     6481 tcccattttc accttttaac catttcgcag catcaaatgg ggttttcgtt cgcagcagta
     6541 attccgtttt gatcgtttca aattctttat cggtcaaagg aaaatcattc agttctgctt
     6601 gattatttga gataattttt tggcgtaaat tgacccaaag gtcttcttct gattttagat
     6661 ccggacgata cgtccactga ttatgccctt ctccaagtac acgaatcaac tgttcttcga
     6721 cttctgcttc ctcacggtga ctaattttca ttcttcttcc ctcctagaca aacatctttt
     6781 gtaagaagct ttttttcgtt tcttttagta aatctaactt acgttgatga agagcgatcg
     6841 tatcatctag ctgtttgaag aaagtaccga ttttttgttg ttcatccttt acaggaacct
     6901 taacactgat attctcaata tcagaactgt tataagaagg caatgcccct acttgggagt
     6961 atcttggcaa atttatagtt tcaaacaata ttttaccaaa atagatatcc caagtattat
     7021 taaataaata agccatggta ttgttatcta ttaagaaagg gcttgtgacc aaacgttttc
     7081 tattaagcat tattgctgcg ccaactttag caaatataat cgctggcaca tcttttattg
     7141 gcttccattt tttacgctca atttgctcat tagatacata atgatttgca tttctcattt
     7201 catactcatt accaatgtta ttcatgtcgg aaactttaaa aaatggagta ccttcagaac
     7261 caccttgctc ggcatcagga aatccaattc ccgattgagt tttacctaat tctcccaact
     7321 tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat ttctggcact tttgctccgt
     7381 tttcagggaa cattttttgt aagaatcctt ttttcgtttc cttcagcaaa tctaacttac
     7441 gttgatgaag agcgatagtg tcgtcgagtt ttttgaagaa tatgccgatt ttttgttgtt
     7501 cttttaaatc attgggaata tagaatactg tatctgaaac ttttttccag tctgaacgag
     7561 gcatttttgt tccagatgta tcatttgcag ccttttgata tctatcagct tgaataagag
     7621 aataaataaa gtccgagtcg ctcttcttag ctctaaaaac ccagaaatcc ccaatcgcta
     7681 cgcctgtaaa agttgccttg agccaatttt ttaaatatgg acgtaacttt ccatatagaa
     7741 tatcatttgg taaaaattca atcccttttc tattatcaaa tttctggctc acatctttat
     7801 tcagtctacc ttctcctgcc acgatatctt caaattcaac ttttggaagt ctactggagt
     7861 ttgtactttt attaagtcta acaactaaat caattaactt acgctcttcc caatcttccg
     7921 taaatcctgg gaaacggatt tctggtcgtg tatcattgct catgtttgaa cacctccagc
     7981 gcaccttgta accacatttc gttttcaggt gttgtttgca gggaagagat agcttctaaa
     8041 agttcttttt ctagcacttg ctgttcttgt cgtatcttct tgatgtcatt tccgacatga
     8101 accatatcaa ttggttcttc ttcttcgaaa gtatctacat agcgtgggat atttaagtta
     8161 tagtcatttt ctttgatttc atcaaaggtt gccaaatgag cgtatttctc gacatctttt
     8221 ctttcagcgt aggtttcgag gattttttga atgttttctt ccgatagttt attttgattt
     8281 ttccctttaa caaattcacg actagcatca atgaataata catctcgagt ttgacgattc
     8341 ttcttcaaca caatcaccgt cgttggaatc gatgtcccaa agaacaagtt cgcaggcata
     8401 ccgatcacag cataaatact gccatcttct agtaattttt gacggatgac cccttctgca
     8461 gcgccacgga ataaaactcc gtgtggtaag acgatggcca tggtacctgt ttccttcaaa
     8521 tgatagaacc catgaagaag aaacgcaaaa tctgctttgg attttggcgc taatttccca
     8581 taacgattaa accgagaatc atctaagaaa gtcgtatctg ctgaccaatt agccgaataa
     8641 ggtgggttca ttactactgc atcaaatgtg taaggttcat ctgttggcca gtccttattc
     8701 aacgtatccc cattgcggag attcatttct tcggcgtcga caccatgtaa aatcaagttc
     8761 atttttgcta gattgtatgt cgttgtattt agttcttgcc catgatattt aacgttatct
     8821 gggtgtgtta agtagttacg tacgtttaac atcaatgaac ctgaacccat cgttggatca
     8881 aaaacactaa agaatcgtcg ttccttttga tcgagcgtta cgatttgcgc catcatatca
     8941 gaaaccatat gaggtgtgta aaattctccg gctttcttcc ctgcttctga agcaaattga
     9001 ctgatcagaa actcatacgc atccccgatc acgtccccat cgtgttctaa aacatctaca
     9061 tcattcaatt tcttgatgac ttcggtaatc gtcacatttc gttgttgctc atctgttcct
     9121 agtttttttg attggagatc cacatcatca aataacccat taaattgatt gtaggtagat
     9181 gccaattgca caaaggcttt gtttagatcg ttcaattgga acgttgcttg ttttgcttga
     9241 tccgctaaga cgttgaaaag atactctggt gcaattgcat atcccaaaat atcaacgatt
     9301 gtggcaatca gatcttcttt tgattcttca tctgaaagta attcacgata caacattgtt
     9361 tgtttgctta ctgtgtcata ttcctctaag gattcatctg ctaaaagaac cacttgttcc
     9421 aacaatcgat cggataaata tttatagaaa atcagtccca ataaataatt tttatattct
     9481 gacgcatcca ttttactacg gagattatct gccgcactaa ataatttttg atttaattct
     9541 gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc tcttatttta
     9601 gcataccccc ttttataaaa agcaaaaaaa aaagaaagaa acttattctt gctaataaac
     9661 tacgcgaaag gagtctaacg ttgtttgata taacttagac tgatcgttaa cttatttttt
     9721 atatttctac atagttatct ttagaaccat ttcagataca tatgaaacaa ttacatatga
     9781 ttttttcata aaaaaacagt actgtctagt agtataaaat atacaaattt cttaaaaatt
     9841 ttttcactaa aatcactacc tgaaacagtc taaaacactt atatatcaac gtttaatgaa
     9901 actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat ccgaaaaaaa
     9961 atacccccag tagcttatcc taagctactg gagatgtata agtacggtca tataacaacg
    10021 gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt ttttaaatac
    10081 ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt tttacccatt
    10141 ttcaagtaac cgaagtggaa tgtctttacc aacttgctct gtcactgcat tactgatacc
    10201 tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct ctgtatcagt
    10261 atttatcgtg attcctttat cttcatttac aagcttccaa gtaggtggta tagctgtgta
    10321 tgtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta cttctgtctc
    10381 attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg cttctgtctc
    10441 attattctca ccggaaactc tcttcacacc caaacttata tattgtccat ctttagtgtg
    10501 catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc tttcaataga
    10561 gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta aaagcatttt
    10621 gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta tacggaatac
    10681 agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc aatctccaag
    10741 tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac tggtagaggt
    10801 cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag ccttagcaca
    10861 accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac acccttgaaa
    10921 tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc tcttcttgaa
    10981 cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc ttcactgaag
    11041 ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg gcaataaaaa
    11101 actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc tttttttcgg
    11161 gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag agcatggttt
    11221 cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg tttccagcct
    11281 cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca tagcagccga
    11341 tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt tgaaaaacaa
    11401 tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta aaaaatagac
    11461 ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc ttgacgcaaa
    11521 taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt agagcatcag
    11581 aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa tcgcctttct
    11641 ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc ttatctaagc
    11701 aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc gttctctcta
    11761 agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt tttttattcg
    11821 ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat aacgcttatt
    11881 gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac caaaaacatc
    11941 tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat aatcaattcg
    12001 atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat agccattttc
    12061 agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta atcgtagtcc
    12121 agtagggtca actacccagc catatggcgt tttatctaca taaggatttt cagctgcccg
    12181 gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg aaacatctga
    12241 tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg caataaaatc
    12301 gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat ataatccacg
    12361 atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta catctaagaa
    12421 agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg gactggaggt
    12481 agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa gattcaactg
    12541 atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa tttgaaagag
    12601 ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag gatgaaggat
    12661 catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat actcagtaag
    12721 caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc aaccgccctg
    12781 ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt cgattccctg
    12841 attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat tggttttatt
    12901 attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt ttaagcctaa
    12961 ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg caacttcaaa
    13021 attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt tcccatcctc
    13081 ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa acttctctgg
    13141 aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt tgcatcaatg
    13201 gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt tcactgttgg
    13261 tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg aaatcggcgt
    13321 tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact tcaaagggtt
    13381 ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata ccttctttag
    13441 aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc tcaacaggtg
    13501 aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt cccttagaaa
    13561 agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga ttttcaacaa
    13621 tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca aatccaatta
    13681 gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt gcgtgaacaa
    13741 tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg gaaacaaagg
    13801 catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca ccaatcatgg
    13861 aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc tcagtaattc
    13921 ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg tttttagttt
    13981 tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc attcctggcc
    14041 cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc gtcattgccc
    14101 aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat ggaattaacc
    14161 aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc agcaacgttc
    14221 caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc agcatcggct
    14281 tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct acataggaca
    14341 tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca gctaataccg
    14401 gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata tttggatgaa
    14461 gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt atggctgccc
    14521 cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta ttcaaaatga
    14581 tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc aaaatggctt
    14641 ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta gaaatgacac
    14701 taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt ttaattgaag
    14761 ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca atcaccactt
    14821 ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct aaggctttcg
    14881 tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa tgggttaagc
    14941 tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact tctctgtaat
    15001 ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc cccaatcaac
    15061 attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc aataacgtta
    15121 ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga atttaatttt
    15181 ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca actagggtat
    15241 atctgcgtat acttactgct aaaataagta tatctaatat taactatacc agtaaaacca
    15301 ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat gcatataacg
    15361 gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg tgagtaaagg
    15421 atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt tttttggtaa
    15481 aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc aatagaatga
    15541 tttgggctag tattcttaaa aattaactag caaatgttta aactgattta tttctaatct
    15601 acatgtagta agtatttcaa ttccaccaat tatcaataaa aataccaaac atatccataa
    15661 taattttttc tttttcattt cttttatatt gttataacag tgaataacat aaaaattccc
    15721 gacaatcatt gtaatatttt tttgtcacct gtattagtat ttaatttata ggaaactata
    15781 aaaactccct agtgaattta cacctttggg agttcctgtt tggacggtat gcataataca
    15841 tttaaatcaa tttaacatta acacctcagc atatggtatc tccacatata tacggtcttt
    15901 ctttggatag tactcaatat ttatgttgat agcacttcca ttgtctaata tagctttagc
    15961 ccggtatgtg ttatctccat tttcatgagt aatcgtcaca tcatcacatg atcgatttaa
    16021 gttgtactgt tcttgtaaaa tctgtgttac caatggtttt gatgacttct ctaatccaga
    16081 tgtacctgtc atttttgaaa aaataatacc tataatagcc aagacaacaa tacctataat
    16141 gacaaacttc ttgttcatat ttactttatt caacttttta tctcctatct ccaaaataat
    16201 ttgttcttat ccctcttatc agtacattga catccctaaa agtacaccaa agattgctcc
    16261 agctacccct gctatcatca aaatcgtacc cattttctca tctttttctc gatataagta
    16321 gcccataata acagcaacag caccaaatac aacaggaata aatgctaacg atactaccat
    16381 agaaatccaa ccaataatca aatacagctt ccctgtctct tgtccatgta cgatttgttc
    16441 cttcatttca ttaattgtct gctcagtact tttctttact gtgtctgttc cggttgtctc
    16501 atctgtttct tgtagtgctc cacaaaactg gcaatactta ctttcatctg ttatttcttt
    16561 gccacatttt ctacaataca tgtcttctcc tcctaaaatc attcaattgc ttattagtta
    16621 actgtttact ttattaaata catcagaaaa cagctcattc attggtacac ttaatatctc
    16681 agaaagcatc ttcatttgat agggtttagg aatcgttctc cctgtttgcc acgaacttac
    16741 acattgctgt gtaacattca ttttttttgc taactccgtt tgagaaagtc ctttttcttt
    16801 tctgtatcgg ctgagattat ttactttttt catgattctc ccctcctgtt ttattatata
    16861 caacaatatg atgtatttca actcataaaa tataaaaaac ttcaaatacc attacaaata
    16921 caacaacttg ttgtactata ctgagatggg ggtgtattaa tgaaacagaa acaatatgca
    16981 aaggtcatta gagaaaagcg aaaagctctc gggtggacac aaaaagaatt agctgaaaaa
    17041 atcttctcta cacaacaggc agtagcaaga tgggaaaatt ctgtaacaga gcctaacctc
    17101 gatagcttaa cagctctttc tagagcccta ggaacccctg taagccattt tctagacaat
    17161 gttgtcgtag attatgagga agagtttttg gctctctatc gttctcttag cactgaagat
    17221 gcagttcgaa caattgacta tatgaagcta ttaaaaaggc aagaaaatga acgcaatcag
    17281 ctattgaaag attgaaaaat aaataaccca ccgaacttta acggtgggtg tattgagatg
    17341 aaataagatt aagagctatg gataacctgt ctagtttaat acaattgggt cttttctctt
    17401 ccttactctc cgtccatcat ctcttgttac acttgtaaat tgatacagag gaactatccc
    17461 agctcctgat aatatcaatt cttttattgt ttcatctatc cctgcccctt tcgctaaata
    17521 tattaaaaaa ctactacttg ctttcacatt gtatagtttc ttaatatcct tagaagctcg
    17581 ttcaagagtg aatccttgtt taacaaagta gcctctgttt tttattaaag catttttcac
    17641 gctgttatcc ccaacattac gtaggcgttc acaaacagtg ttaataagtt ctttctctgt
    17701 cattagatat gtcccttgcc acgataagcc ctgtaaaact tcatgtgctt catctaaaat
    17761 caattgtttt tccttgctga gttctgatcg ggaattttga ggaaatactc ttttagcctc
    17821 tttttcgtta aaacatatct taacccattc ataagaaaaa tattgatatg taaaatgaat
    17881 tttttccaga agtggaagga tatcttcatt caatttttga atcacagaag ttgcattaaa
    17941 aacgtctaat cgatagacat tgcttctctt atgattaaag tttcctaacg ctttctcttc
    18001 ctttaaccta tttaggtatt cgagcatctt caaaggaaca tcttcatcat ctaatttttt
    18061 tatcaatcct ataaaagtca taagtgatag taacttatta catttatcta tgtcataagt
    18121 tttaccatag taagaactca tttttttagc taattgttcc cccgacaaaa tacaagtcac
    18181 actttcctta ctatcatcaa aaaaattaac atcagaaagc aagacatcat agacacgtcg
    18241 catttcctct agataattcc ttattgtcct gaaaagtttg tctttcattg attcaagagt
    18301 gtcaaaaagt acttttgatt cctctttcaa ttttcgaaaa tgtgtaggat acaagtctag
    18361 gttctctaat agccaattaa atgtttctac ccgactgtct ttctttaaaa ttagcatcac
    18421 aactccaata aaattttcac tgatatcaca attactggaa tgacaataaa aaatggaatg
    18481 cccagtttta ctggtaaaaa tactagcaga gggattatga tcgttgtgaa acagacatct
    18541 taagttttta cttgacttaa gtaatttatt catatcaatg gttaacaatc tttcataaat
    18601 ctcattaaaa gacatgtcgg aggaatcaaa caggcagtct ttaaaccact gtttcatttc
    18661 ttctttgtcg tcatttttta tcaacttaac aaaatcggtt tcattgacta gttcatactc
    18721 tctccctgaa tcactttgac aatttttaga acatgtcatg gtaaaatcaa tcggaagctc
    18781 agtaatatct aacatgttgt cttcattaat tattatcact gcatctgact tatatcctcc
    18841 aaaaaacatt ctattcgaag catttacatt gtcatcacac ccggggatgt tctttttcaa
    18901 gtattcgtaa acagcaacca ttttaccata gtcagtaatt gcttctttta atagaaagac
    18961 aatcttgtat ttttctagat tttcagagta ccgaatactt ttttgtatcg caaacgcaga
    19021 attatgaact aaattgatag ataaaacctt ttctatgctt aagtatctac tatcatcttt
    19081 actcaaatct actaccttcc ccttctcatc tttaacagtg ttatcgacat ccagaataat
    19141 tactctggtt tccacaaaac tctccttgtc attgccttta ttctttgtct tgcttggaat
    19201 aaatgattgt gatgaattta gtatctctac aaactcatct cttgtaactt ctctatagtt
    19261 atctaaacac ctatctctta catcagtttt ttcttttttt gtaactggct tctctaataa
    19321 tttttcttga tctacataaa ttggaatatt aatcattgat gtttccattt gtgtttcctc
    19381 cttataaaat tctgttaaag actaaaacat acagataata aaaacaaact ctttattact
    19441 atatgtttta gtcttatttg tttactcaac ctttataccg ttccatgaaa ctatcaatat
    19501 cagatttttt aaatctaatg accgtatcaa tttttataac aggtaacccg tattgagaaa
    19561 catattttct aatcgttcct actgatacac ctagatattc acttgcctgc tgatagtcca
    19621 tatactctag agtgactcca gaaataatgt cttctagcat aggctttagc actgtaatta
    19681 tctctttcac ttgttgttca acttgtatag aaacaatgct atcaatgatt tgtagagaca
    19741 tatttccccc tcctttcgtg attgcttgaa caaattttct aagaattatc ttcgtatttt
    19801 ttgctcaaaa taaaagtatt ttgttattaa ccgattaatc aaacaaaaaa tgattaccag
    19861 ttcatcgaca tacacataat acctcgataa aaaaggggtg tcaacagctt tgtaaaattt
    19921 atttacaaca cccctttttt gaggtatact aattttagga ggtgtggtca atatgataaa
    19981 acgtccacca ataaattatt tagaacgaaa aaagatactc ggaacaaaaa taaaagctat
    20041 aagaaagagt aaaaaattaa cacaaccagc atttggtctt atgattaaca acggtcaatt
    20101 aattgacaaa aaaactatct acgaatggga gaagggaacc tatttaccaa taccagagcg
    20161 attatcccga attgctgatt tgggaaatat gagtatagag gagttagttt gtggcaatgt
    20221 agaagaatat attttgggaa ttattcttta tagagatagc attgtactag atggtataac
    20281 atttccagat aaaaacctgt tccagcatct tcgtcaacag ttcccaccag tacactctaa
    20341 tttagataca tggctggata gatattccaa attagaacca gagatgcaag aatttattgc
    20401 taacaagact tgtaataaag ttaaaaatga aaaaattagt cttttcaata tcttaaaaat
    20461 agaagaatta ttcatcaatg ctattgtaga agaatttgac aataacattc ttttcctcac
    20521 tagcagtata gaagaactac tagaaagaat ggtagatgaa tggctaccta ttcaattgaa
    20581 agacatgtct tatcccgaag aagctgtgag agaaattact gataatataa acaagctaga
    20641 acaaacgatt tcatcaattg ggaaaaaata cactaagaaa aaaatgaagg gaggtgatac
    20701 catctagtgg taagtactcc ttgtaaacta agaggagatg atttaatggc aaaaatatca
    20761 aagtacaaaa agaaaaacgg tcaagtggcg tggatgtata aaggacacat tgccaccgat
    20821 ccacgaactg gtgaaaaaat aaatactacg agaagaggtt ttagtagcaa acaagaagct
    20881 caaaaagatt atgacgagta cagacatcaa atattatacg gtgttaaaaa gaaagcacct
    20941 gatatgacat ttgaagacct gtataatgaa tggataactc atcagcgtac cagtgttaaa
    21001 gcttcaacaa tcgcaatctc agtaagatac gcaaataatc aaatacttcc agcatttggt
    21061 aagcttaaac tatcgaatat ctctgtccca tattgtcaaa aagtggtaga tgaatggcac
    21121 agtaaatatg aaagttacga ttatatgaga aaacagactg cacaaatttt acgctacggg
    21181 gttgcaatgc aatatattga caataatcct atggaaaaaa cacttcttcc acgaaaaaaa
    21241 gagtatgaga aaaatcgtaa attttactca aaagaggagc ttaataattt actggatgct
    21301 tttaaagatt ttggtaacat gaaacaatac gccttcttca gattattggc atacacaggt
    21361 atgagaaaga gtgaagtact cgctttacag tggaaagaca tagacacgtt taataaagaa
    21421 ctacatgtca ataaaactct tgctgtagat gaatttggta aagttataat tcaatctccg
    21481 aaaacaagag cttctagacg agttatctct ttagatactg agactctttc tatcctcaac
    21541 aactggaaat tacagcaaaa agaagagtat ttaaaacttg gatacaatac tagctctaaa
    21601 gagcaacatg tttttacaac agttaaaaac actctataca ttccaaacac tgttaatgat
    21661 tggttaagat atattttgaa aaaatataac ttgcctcgta ttacacctca tgggtttaga
    21721 catacacatg ctagtttatt attagaagca ggagaatctg tcaaggtagt tcaacaacga
    21781 ttagggcatg aaaatagtaa agtgacatta gacatctatg cacatattac aaataatgca
    21841 cctaagaaaa caggtcaaga ctttgctgat atgatggctc atcagtaaaa gagctggtca
    21901 aaatctggtc atttctagta tacaggcaaa acaaaaaagc tagaaccctt atatat
//
LOCUS       DACECB010000050        21923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.050, whole genome shotgun sequence.
ACCESSION   DACECB010000050 DACECB010000000
VERSION     DACECB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.050"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(602..675)
                     /locus_tag="IWO04_001907"
     tRNA            complement(602..675)
                     /locus_tag="IWO04_001907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..1884)
                     /locus_tag="IWO04_001908"
     CDS             complement(865..1884)
                     /locus_tag="IWO04_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0213509.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1895..2092)
                     /locus_tag="IWO04_001909"
     CDS             complement(1895..2092)
                     /locus_tag="IWO04_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0213510.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(2108..2350)
                     /locus_tag="IWO04_001910"
     CDS             complement(2108..2350)
                     /locus_tag="IWO04_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0213511.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(2428..4626)
                     /locus_tag="IWO04_001911"
     CDS             complement(2428..4626)
                     /locus_tag="IWO04_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213512.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPYQPNLLVEPYNMCREYPNENIADPKVKF
                     PIESGDHQIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLT
                     DVWSLTFTPTNVVAMPKLFRVYQYPRSTVGACQIDWLKIEKGNTRTPNISEYKYRGTG
                     MRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEV
                     DTTAKDAQTKANELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVAYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD"
     gene            complement(4650..6941)
                     /locus_tag="IWO04_001912"
     CDS             complement(4650..6941)
                     /locus_tag="IWO04_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213513.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREVA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(6951..7688)
                     /locus_tag="IWO04_001913"
     CDS             complement(6951..7688)
                     /locus_tag="IWO04_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0213514.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(7739..11071)
                     /locus_tag="IWO04_001914"
     CDS             complement(7739..11071)
                     /locus_tag="IWO04_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0213515.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKVQNDNASAWR"
     gene            complement(11089..11271)
                     /locus_tag="IWO04_001915"
     CDS             complement(11089..11271)
                     /locus_tag="IWO04_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213516.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            complement(11274..11636)
                     /locus_tag="IWO04_001916"
     CDS             complement(11274..11636)
                     /locus_tag="IWO04_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213517.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            complement(11656..12264)
                     /locus_tag="IWO04_001917"
     CDS             complement(11656..12264)
                     /locus_tag="IWO04_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0213518.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            complement(12276..12680)
                     /locus_tag="IWO04_001918"
     CDS             complement(12276..12680)
                     /locus_tag="IWO04_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213519.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(12673..13074)
                     /locus_tag="IWO04_001919"
     CDS             complement(12673..13074)
                     /locus_tag="IWO04_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213520.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(13064..13417)
                     /locus_tag="IWO04_001920"
     CDS             complement(13064..13417)
                     /locus_tag="IWO04_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0213521.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(13407..13718)
                     /locus_tag="IWO04_001921"
     CDS             complement(13407..13718)
                     /locus_tag="IWO04_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213522.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(13715..14590)
                     /locus_tag="IWO04_001922"
     CDS             complement(13715..14590)
                     /locus_tag="IWO04_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213523.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(14600..15760)
                     /locus_tag="IWO04_001923"
     CDS             complement(14600..15760)
                     /locus_tag="IWO04_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0213524.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(15760..16446)
                     /locus_tag="IWO04_001924"
     CDS             complement(15760..16446)
                     /locus_tag="IWO04_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0213525.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(16409..17587)
                     /locus_tag="IWO04_001925"
     CDS             complement(16409..17587)
                     /locus_tag="IWO04_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0213526.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(17607..19301)
                     /locus_tag="IWO04_001926"
     CDS             complement(17607..19301)
                     /locus_tag="IWO04_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0213527.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(19279..19593)
                     /locus_tag="IWO04_001927"
     CDS             complement(19279..19593)
                     /locus_tag="IWO04_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0213528.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(19696..19977)
                     /locus_tag="IWO04_001928"
     CDS             complement(19696..19977)
                     /locus_tag="IWO04_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213529.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(19982..20326)
                     /locus_tag="IWO04_001929"
     CDS             complement(19982..20326)
                     /locus_tag="IWO04_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0213530.1"
                     /translation="MTEEFYRWLLQLIREDCLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(20586..21515)
                     /locus_tag="IWO04_001930"
     CDS             complement(20586..21515)
                     /locus_tag="IWO04_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213531.1"
                     /translation="MKFDEAAFVVKGKKEIISLEEIPRRKDFLEIVQHIYCPDPSCEA
                     RLVFNRRSTGVNYLSKHRSDEHNIDCAYFEDEIKPVKSITEYSEINGGLTDGGKLRRK
                     KESMKALRDYLNPPKPKPKAKNSHKKTTQKKEITEGTETYQGIKVKYDPDGEVVKKDT
                     GNGEVKVKEPPFYERLPHQLSEKDSGQNLRTSAIIKEVLIFENDVPRAEIKANFDDIN
                     ITFVMPEVFFVSNERRLQVDQLIEYLSIIKNYVKKHPNYLFLTTMCQSRKLDMEDIIL
                     YILEPDFMSFQTMDGRDFSTLTSVIAAISTKVI"
     gene            complement(21679..>21923)
                     /locus_tag="IWO04_001931"
     CDS             complement(21679..>21923)
                     /locus_tag="IWO04_001931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213532.1"
                     /translation="LNNIIYSMKNEKMYLEEWELVDIPCEKVYCFYNKKRKEAFDFEE
                     DENGILAPHYYCVREGTKITKIPTKTIKEAIENYQL"
BASE COUNT         6305 a         4542 c         3260 g         7816 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat gatttttata gttgtcaata tagttatgta
      841 atgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 gacgtatact ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt
     1021 tcctaaagta ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct
     1081 agctagttct tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc
     1141 aatgtgccat ccagctacat gcaaatttgc attttcgatt tctccaaact gatcaatgtg
     1201 ggcgtaattc gttccatctg tcagtgtagg attagctgca ccagctcgtg ttggatcaat
     1261 gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag ctaaatcata
     1321 tgctaatttt tctttactta cgcccatttc agaaagataa ccgtatggat cagtatgatc
     1381 tccccagata ttttgtgtta cccataaatg tgatttgatt cctggttggt tataaggcgt
     1441 gtccaatgta agcggaatac cgtatttctt agcggaatct ctcgccaatt caacgtatgc
     1501 tttatagttc ttttcaaaag ttgctttatt atgtgtgtgt tgtaactcaa tctgcacagg
     1561 actgttggca ttagcatacg aaccagcacc gtactgtaca taaccaggtt gtccaacttg
     1621 gtaaacaatt ccgccatcac ccacaatgta agcagtgtaa gcactagtcc atgaacgttg
     1681 catatactgc gcttcattgc gtcctgttgc tgtttcatta gccgtttcat gcagtaaaat
     1741 gtactgatta tttgctactt gtgagctacc ttcatttgcg cccaaattaa attcattgtt
     1801 gatagtatag gcaaacccat taattggcaa taaaaaaaga gccgttaata ggctcatcgc
     1861 agtaatagta attttctttt tcatttgttt cctcctattt tttcaaatta taagccgaca
     1921 caccagtgat aacacctaaa aatgttgcta ctgcattgat agtgagtact gtcatatctg
     1981 ttccattcca tccatacgct ttccctaacg tcgctactaa tacagatgca gctggcaata
     2041 ctgttaaaac tgtccattta atgacttgat aatacttatc tggtaaaacc atttcttctc
     2101 acctccttta caatttagtc aagaaatagc caatgattgt aatgcctaaa ccgatcatgt
     2161 aaccccacga ccatttatta ttggctttca tctctttgat gtcttctgca ttattaagcg
     2221 caatagaata tgcctgatcc gctctatctt ttgcactttc cgctttttca cgtaataatt
     2281 cgtaattatc cagtttcgtt tcaatacgca ctaagcgttc taccacgtct tgtattgctt
     2341 cgtctttcaa ccaactagcc tcctttcatt gcaaaataaa aaacaccctc ctttgagcgt
     2401 gttgatagca aaattataaa tattgtatta atcagatagt ctagtcaaga ctaaagtacg
     2461 gcaagatgta aattgtaaaa ttccatctgc aacgccgatt tcagtacgca tatttagtac
     2521 atcgtttact tctaaatcag ttacaatgtt tccgctagct gcaaatctat tttgcaaggc
     2581 gtcgacccca taagctacta aattatcaac agaagttcca tctttatata aatcagtata
     2641 taaccatgaa gcatatttcc cgcccaattg cacacgtatt tgcgcactaa ttgaatactt
     2701 accagctttt aagcatttaa cgtatccgtt agattgacgt tcgaaatgtg tttctcctac
     2761 agctgtagta gtataccctg ttcctactcc atactccgct actttcgttt tgttagagac
     2821 ttcaccttgt gcgccataaa atgcggtagt agctgtatca ttaatgcgtt catcgatctt
     2881 attgtctaat tcattcgctt ttgtttgagc atccttagct gtggtgtcta cttcattaat
     2941 tgaagcagtc aactgcgaat taatctccga tactttccca tcggtataat tgtttgcttt
     3001 accagtaatt tcagaaattt taatatctgt ggccaaatta tcttcgacat attctggtgc
     3061 tagatcccaa acataatctt ttggattgtt tgaatcacgc ataccagtac cacgatattt
     3121 atactcacta atattcgggg ttcgtgtgtt gcctttttcg atcttgagcc agtcaatttg
     3181 gcatgcgcct actgttgatc gtggatactg ataaacacgg aaaagtttag gcatcgccac
     3241 aacattcgtt ggtgtgaatg ttagagacca tacgtctgtc aacccatcaa ccggctttag
     3301 ttctcctaaa cgagcagtcc aatgattata cgctacaaag gtttgacttg cgggttttgt
     3361 tcctttaagc gtgatagtat acgtttgacc tatcataagc tcttcttctg tgtaaccttg
     3421 atatatttgg tggtcgccag attcgattgg gaacttaact ttaggatcgg caatattttc
     3481 gttaggatat tcgcgacaca tgttgtaagg ttctaccaat aagttaggct ggtatggtgt
     3541 ggctgttgga ccttcttcga ttttgatgtc atagctcatt tctatttccc caagtatccc
     3601 atcttgagcc ctaaattgta tccaaggatg gtatgcttgc gggccaaatg ttggagtagt
     3661 tccagtaata ctaaactctt tccattcctg agttatcgaa ttactgttaa taggtaagtc
     3721 taaaacaatc ttgccagttc cattgtcaac aaacctatat ctaatcagta ttttttttcc
     3781 gtcagctgga ctatagtcaa ctccagctct aagtttaacg gtcattacaa gcgttttatt
     3841 agagggtaac cagttagcta tacctcttcg tttataatat aactctactc caccttccgt
     3901 ttcagctgat atcttattat atttaaacga gttttcacct ttagaaagtg ttccagctga
     3961 agtattttga ttttcaatta agtcgtatga tagcttactt aacaaattag gattcccact
     4021 ataatcatag ttcccgaaat cgatgctgtt actgtacatc acttgtaagt tacctaactt
     4081 agaaatttct tctttcagag catctaactt gtcttgtagc gttttagctt gaccagttaa
     4141 atcagtaatc tgttgattta agctatccac tctaccttta gtttcagcca taaaagcatc
     4201 aaaagtttca ttatactttc gaatcaactc ttctaattgc gaaacatatt catcggcttg
     4261 accttgcgaa atgtcagaca ctcctagtga gaaaaaaatg atatcttgcg ttgttagaat
     4321 ttgattgtct tttctatatt ctacgtagca gtgtttataa tatcctgctt cactcataaa
     4381 tgtgccatca agagaaaacg tgacttcttc actagttacg ctagttgcaa cattatctac
     4441 gtaacggtta gatggtgttg ttccttttaa agtaaatgtt ccgccactcg tatccatctg
     4501 caagccattt aaatatggtt taaccgtcac cgtaatccct ttatcaccct gacgagccat
     4561 aatagctttg gtgtagttta attctttgct gaaatctaaa gccaaattat ataaactgct
     4621 agccatttat atacctcctt gtcttcgttt taaaaacgtt tttggtcaag cactgtgcta
     4681 tcatatgctg tatcctcttt taatctaata tcttcatacc ctagacggtg tgccactaaa
     4741 ttccatctaa ctaatacgtt tggcttacta gtttcaatga tgaaatggtc aatatcttca
     4801 tgagtaacag cacacaaaac tagttctgta ggtgtcacat gtgtcatata ccgacttaga
     4861 tttactgtct cagcaaacat ggggtcaata tcaacacgaa ctttaccatc gtcacctgta
     4921 acggcttccc cataatcagc gaaataatat tctggagttt cataagcgtt caatagtcgt
     4981 tgtccataat gttctgttgg tacagttgag tttttagtac ctctaacagt aaaatcttta
     5041 tatacttgta ccgttgattg ttcaaaccta gcaagtttcc catcttccca tgaaccaaaa
     5101 aaacaacctg gtaacgttag cataccatca ctagtaaatt tcatagtcct accagctacc
     5161 ttaaattccc atgagttacc cgcactacca ttaatgctta aagaactacc gtcgccagaa
     5221 gttacataac tagcattgct atacctgaaa ttgggcgcac caaaagatag aaacggtctg
     5281 ttattaccat tatcccacgt actaaaaacc aagttaccct gtggatttct aatcatgaaa
     5341 ccaccaccag ttttcatggt gtatgataca ataccggcat cagcacttac ataatcacgt
     5401 gcttctagct ccataatatc tttgttaact ttttttgagt accaagtcat tttgccatta
     5461 gcaatacttg ttctataatc agcaccatca ctaattaatg tagtacctct aatagtaatt
     5521 cctactattt caccagccgt aataaacgag gcattgaatc cgccatctaa cgtccatgcc
     5581 gtttcatatg ttccattaat gccagtttta gaaaaaccaa taccagcatt gttgatttgt
     5641 aaaacattcc ttgcggtatt cttatctggt gtgtccataa tcaaaatacg actaggcgct
     5701 tctttaggat ctaataaaac ataaccacca ttttgaccag taatcatatc agtttgatga
     5761 tctacaatat cattgattaa atcactgatt tcgccaacat ttttcaattg atcaatggca
     5821 tcattaatca aattgctgac attattctct gtattttcta agaagtttgt tttgacgttt
     5881 cctacaacta atttatcgta tgaattagtt aaaacattaa acgtatattc cacgattctc
     5941 gctgacatat tcacttttaa ctgtggatga tacacatcta ctccgtcacc catcgaaact
     6001 ttttctagat caacaaattt ttcatagcct ctttgatgcc tcaatggtac taattcaata
     6061 gaaccactca cttgtggttt ttgtttatct atgtttgttt tcaaccagtc tttagcaact
     6121 tcccttaatg tggctacatc agtcgctttg tctttaaaat caacaaaaga aacatatcca
     6181 gcaggataat catccacgta atccgtgaaa ataacttctt ctggtagagt gatctcgtct
     6241 tctccttctg aagaactgct aatgaatgga taaactccaa ctaaaacgct ttgagcatct
     6301 atctctaagt caagaccagt taagttttta gtataaatcg ctttgatttt atgatccgta
     6361 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
     6421 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
     6481 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
     6541 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga ttttttctgt tccgctgtca
     6601 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
     6661 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
     6721 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgttt
     6781 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
     6841 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
     6901 gcgtcatttt tatcgtaaat agttagtaaa ttatcgatca tctatttcac ctaccttaac
     6961 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaatgatt aatatccact
     7021 tgcaacgttg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
     7081 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctatgtcagt tcccactact
     7141 aagccttcaa aactaaacac ataatcattt aaggtgaatt ggcatgaagt agctgaagga
     7201 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
     7261 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtgata
     7321 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     7381 tggataacat aatagacgtt gggatcatca gagaattcta atttctttgc atagtttaac
     7441 caatgacgca tgatataaaa cgattgcttg aatgcttgat ggtcttccac atcttctaaa
     7501 tagttatagt gcaatgtaaa cgacatgtct tcaaacgagt aatcttgtac taagccacct
     7561 agccgtccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     7621 tccgctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     7681 tcagtcattt tttcacatcc tttttaaaac taaaaaaaca ggagaaatac tctcctgttt
     7741 aacgccatgc cgaagcatta tcattttgaa cttttgtaat gctatcaatg atttgttgag
     7801 ttgtttgctt catagtaacc tcatctgcgt taccatcaat tgtgaaattg aattcgtagt
     7861 tattcacagg ttgaatcgtt tgtgccctag atgaaactga tgtgctactc aagatacgat
     7921 caccaatttc ttgcaaaaca gatcttttca aaggtaaaac tgcttcaggt cctgcttcac
     7981 cgacaccgtt cattccacct agtaaagttg gtttagtaaa gatacctcct ttagcatgcc
     8041 attttacacg caaatggggg atttgacctt ttagcgggtt aaagctgcct tccatgataa
     8101 attccggtaa cggaatatgt ggtatagaaa tattcaaatt atcaaaaatg ccactgattt
     8161 tgtctctaat ccaatcaatt ggagcgctaa cagtcttttt gatgccttcc catatgttag
     8221 caattgtact tttaacatta ttgaatatgt cggaaacaat acctgttaga ttggaccaac
     8281 cgcttgaaat tgcatttttt ccatcgttta ctttagagct aatagtgctt gtaattccat
     8341 tccaaagatt caaagcagta tttttgatac cgttccaaat tccgctaatc cacgaagata
     8401 tactattcca aacactttga atggcacttt tagctgcgtt tatagcattg cttatactac
     8461 tagtcacact attccagata tttgatgctg tagagctgat tgaattccaa attccaccta
     8521 accaactaga tacagttgac caaatatttt gaattactgt agcagctgct tgtaccaagc
     8581 tagtgattgt attcttgata ctgttccaaa tactagaagc tgttgcacta attgaattcc
     8641 aaatatttga agccgtagta ctaatagctg tccatatacc attccaccat gccactactg
     8701 gatcaaatat agtatggaat gtagttacaa tcccattcca agcgatactt atccattgtg
     8761 tcatagtatc ccaagtattt ttaaggaaat cagaaatagg tgtccaaaca gcttgccaag
     8821 ctgcgcccaa taactgtcca gctacatcaa aaatacccac gataatatta ataccagctt
     8881 gaatcaatga cgttattaat gtccatggta tttgaacaat tcctacaatg tctgcccaaa
     8941 taatcgacca tacttctttg actccgttcc aaatatttga aacccaatca acgaatgctt
     9001 gccaagtctc ttggactcct tgccagatgt tggaagctcc ttcaactaat ccgctccata
     9061 gctctccaaa ccaatcagaa actccttgcc aaatatcttg aacccaatct acaaatccag
     9121 accaggtttc tttaacccca tcccaaactg atgaggcact ttcttttata ctttcccagg
     9181 tatcacctaa ccaatcggta aattttttcc ataaatcact aaaccagtca gtgattgcgc
     9241 cccagttttg aaacgctgta attacaatcg ctattacagc tgcaacgccc gctataattc
     9301 ctattattgg cagcaaaact gtagaaccaa atgtgccaac tatcgtaaca actgcggtta
     9361 taacaggagc taatgtgctt aataatgcta gaattcctcc aagagctaaa atgaaatttt
     9421 tcattggccc atcaagttta ctccaccatt cggctaatcc ttgcaaagct ttcgctcctg
     9481 cttgcaaccc tttgataaac acaggcagta tatcttcacc caatgaggca taaaaatctt
     9541 ctaacgcttg ctttgctctt gtcatttggt tttctaaacc atcagactcc ctactagcct
     9601 gacctgtagc accggctaat ttttgcatat cttcagcata ttgaactcga actgcttgct
     9661 tggtagcctc atctaaatca gaccattttt gcgtttgtgg gcctaattct tcatttattt
     9721 tatcttgcgc atctttaagt tttaaagcag cctctcttgc ttctaaagat ccttcaccat
     9781 gtttcttaat agcatcagca tacttagatt gtgctttttc aacagctaac aatgactctt
     9841 cactggcttt ttttgctcct tcagtcgctg gtatcaaatt atgcttaaca gcataagctg
     9901 ccatttgagt atcattagca aataaaccta tttgctcccc accttcgtag ttccctttta
     9961 taaatgaatt aagagattca ctagcatcat ccatagactt atcgtaaaaa gctgctgcat
    10021 ctgctgctaa ctgagtacta tcaccagcta actccatagc ttctttggta tcatatccaa
    10081 gtcctttaaa cattgacgta tattgtgtaa aaacaggctt gatcgtattt ggtaacattc
    10141 caaattcttc agccattccc tcaacggcgt cctgtgcttc cccttctaaa gagccaaaga
    10201 cttgtttaaa ttgggcctgc atagcttgtg cttttccagc tgcttcaata gacttactac
    10261 ctacatcaat aagcttatct ccgatcactg acaagtgatc agtagcttcc attaaattac
    10321 ccatatcaag ttttttgccg atatcatcta ctgtggaggt atcaacgttt ttagcagcat
    10381 tacttaattc ttcgaattct ctttccgcat cattaagctt agttttcatt tctaaagctt
    10441 cggtagatgt ttctccaaat tctttttgtg tggcatctaa ttgttttttt agcacttcga
    10501 ttttttgttc agcaatatca ctctgtttgc cgacatattc ttgtgctttt gctaattttt
    10561 cagattctgt agcagactgg ccagcagttg cttgccattt tttatattcg gattctacta
    10621 atgatgcgct tgactttaga tgttgttgct cattatctaa gtctttcata gttgactcat
    10681 acgttttaaa ttcaccttta gattgagcca atgccttact cgttttatct atatcattag
    10741 ataacctttg ctgagctgtt tgttgattaa tcagttctct ttcaagtttc tgaacttcgg
    10801 tggaattttc tccataatat tttttggcat tggctaaacg ttgactagtt acttcaactt
    10861 tttgactttg taattcatac tgcttttcta aagaagataa tttacttcct aacttgtctg
    10921 attcagaacc agtctgttgt aattgagctt gttctagttt taattctgct ctatttttag
    10981 ttaattcagc actgatttct tttaacgtag atttcaatcc gtcatcgtta gctatgaaag
    11041 ttacttctgc ttccgttctc tttttagcca ttttttacct cctttccttt agtttttctg
    11101 ggattgattt attgcatagt tcttccatcc ttcataagcg ctcttgttgt aagccatttg
    11161 caaaatgtca tctaaacaga tatcgcttaa aaccaaatct gaaggcatag aaaaaacgtc
    11221 ggtcaacatc gaatagacat cgacccacgt ttcaactaag agctttggca tttttacttt
    11281 tgaagctttt tttccttatt tgctttttcg aattcttttt gataggcatc acgtgcttgt
    11341 ttgaacatca tagtactgta tacagctact gcaacctcca tatcaaaatc ccatttatcg
    11401 ataaattcat cgaatgaaat gtaatcgacc atgttcgctt gacgataagc tacatatacg
    11461 gcttttgcac cttgaataac tgtaatatcc atagagcctt ttcccatcga catttttgca
    11521 aactcgtctg tgttaaaatc tctattgatc atcaataatt tcttgatatt cagtttaggt
    11581 tctaaattca aaattgttcc atcgtttagt tcaatttttg agtaatcttc gttcatttcg
    11641 ctacctccgt tttttttact gtgattgagt ggccgtagtt gtcacaactg aagttttttt
    11701 aatcacatca gcagatagat tcgtcatcca ttgatctgtt aagtcttctt caagttctgc
    11761 aacaattgct tcatgataaa atttaccaaa ttcatcttgc ataacttttg cttctagttc
    11821 taacgcagct acttcatccg caccattttc aatagagaat gttaatcctg tattcgaagt
    11881 gcatgctaac ataccaacta actttctatt ttcttcgaag tcatccacga tctctgcagc
    11941 aagtgagaaa tcttcgccta cggaatcagg accgtaagag taaatgcctg gtttaatacg
    12001 tccatcttgt ttcaacccat tgaaacgtcg ataaacttcc atcggtacat gtgcagtaat
    12061 tgttaccgtc atattgattg gtttagattt tgattttact tctgtcgctc cacatttttt
    12121 aaccaccgtt tgcatttctg tttcgccatc taattgtccg ttacaattcg ttgcgattgc
    12181 atttcctgcg ttcttaaaat taaaagcaat tcgtttgata cttacgttat cgaacgttgt
    12241 tactacagtt tttgttttag ccattgttgt tccccctatt tatttaattt atcgaattga
    12301 cgaatcagaa gttctgtaat tggatcaagt gcaagaccta atcctcttct cataaattcg
    12361 tccggctgat ttcttttaga agtacctatc cccaaatcag gatattttaa atactcaaat
    12421 tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt gagtcttaag tggacgacta
    12481 ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag aaacaggaat tttttctgta
    12541 atcctatcca ctgcaatagc cgaacctttt gatttcaatg cttcgttaat cagtcgttcg
    12601 ctctcgcttg aatagcgttc catccgcaca agaagttcat catgtccatt tatttttagc
    12661 tcccaactat ttttagccat gacaatcact cttcaataat cgtctaaacg taaataccaa
    12721 ttgatcgata tagcgatctt ggttctctag ttttaaatga ttgggatcca ttctctgaaa
    12781 acgaatcgaa cgattttgaa tcaatgaaat aatatctagt gagtctcctg ttaaatcttc
    12841 tctattttct gaatagaaag ttagatatag attttgaccc acgctatatt ttggctcagt
    12901 gatcatttct atttctcctg tttcgagaat gaagtaatta aaatcatcag gtagctcatc
    12961 ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc tcttctaagg aagttttgat
    13021 agcagaaatt tgcttattta aacgttcttt ttctttagaa ttatcaatca ccatattcac
    13081 ccacactttc aagataaaaa tagatataaa aattatcgta atcggcatag ataacgttgt
    13141 aacgcatact atcgattacg ataaaatatt gatctttatt aaatttcttg gcgattggat
    13201 gaaatggagt ctttactttc ttagttaatt tcgatcccat cgcatccata gctgttatat
    13261 cactatctct catggaaaga tttctaaatt ttaaagaagt gatttctgta tcttctacac
    13321 caatcttttt tcctagttca tttcttttgg tagtttgcgt caaaatcttt aaccaaccat
    13381 cgttgaatgt ttcttcgagt ctacgattat tcgccattca catcacctgc aatatattct
    13441 tgtagcgcat aatgttgaat gaaacctaat aactcactag cgaaattttg ttcaaactca
    13501 tctaaagcac gattccagtc gtatctacat ctttcgatta gcaatccgta ttctaagctt
    13561 tcaggagaaa aagaaagtgt tgtactcact ttactttgaa gataaacagc atttttagct
    13621 atcatctttt taattgactc atcttcttcg ttccaggtaa cgtaaatatt atccttcaca
    13681 gctattagca attcttcagt cacttgttca ggcgtcatct aaccaccgcc ttaattgctt
    13741 taacatatgc gtaagagcat tttttcttgt ttacaaatga taaatcttca tcaaaaggcg
    13801 tagaagtcac gtatctccct ttgaaaaata aatcttcatc gtttgttgtt actccagcat
    13861 tgtgtaagat ttttacttct ttaacttttt ctattggatc agtagcaaaa caaaagtcta
    13921 attcctcgtg aactttagga ccaatattga aatacatcat gttccaaagc tgtgcccaca
    13981 tctcggctgt ccagatttgt atatttgttt tttgccctct aaggtagcga tatagccgat
    14041 tagaatccag ataaaccttt ttccaataat tcgctttagg acggttaata acccactgtg
    14101 cgcctcctga attagtgttt atagtttcca aagattctac tgtaacattt acaatgtttg
    14161 ccatatcttt tagaatattt tctccgtttt cacagcttct aatataatca agacttagat
    14221 aactacagca gtcgctacaa taccaaacat catctttaga aggcaatttg cgcaaattaa
    14281 ttcttttatt gaaaatgaca tccgaatcga tatagaaata tcggtcgtcc tcacgcgaat
    14341 gatcttcttc taaatatttc caccataaat atggtttaat cgaaggaata tactctttgt
    14401 cgtcccgcag atcatcgtac acatgaactt caacaccata ttccttctca aaaaaaatag
    14461 gaatctgatc atcgtgtctg ctgaaaagca atacgatatc tttgattcct agtttcttca
    14521 gattagttaa acaaacttca agctcccatt taaaccgatt gattgccggc tgacaaagaa
    14581 tatacttcat tctgatcacc tacgcttgtg ttgtagttgt tgtggttgtt ggttttgtag
    14641 ttgtagtagt agttcccaaa gcgctaatat ctaatacaat gaaactatcg ttacgtttag
    14701 gttgaccgtt tgcatattgt ttagctagat aaatgcgttc gtcttcaaca aaatggtatt
    14761 catctgaagc ttcaattttt agtgtagatc ctacacccat gaagtaatct gaggctaccc
    14821 caataactgc ttttccttct ggcacagccg ttgactgcaa atctgaaact ggtactggca
    14881 atacttgtac gtattctcca ttagcagtta gtacagtctt agctgggaat actttagacc
    14941 agtaatcagt tggattcaca attaggacca catcagaagg attcacatta cgataaatcg
    15001 gatcattcac accttcgata ttgaattttg atagtcgcgc catcaaaccg cccatagtta
    15061 cagcatctaa agctgtaata ggttctgctt ttttttcagc atattctccg ctagtttgtt
    15121 tgctcatgtc acgcatcatt ccgactggca tatctttacc agtaccatca acaattgctt
    15181 gttctaatgc aattctcaat gattctacta aaacagtacg gacataacga tctaaccata
    15241 ctggacctaa atcaagcatt gccttacata caggaatata acctgatagc ttgaactgct
    15301 tcatgttaat tacatcaaag ccattatcta aaactttttt aacagcttcg caaagtttac
    15361 cccaccatgc tggattgact ccacgtgaca caatccattc tgttacacca gttgtgttaa
    15421 caaaagtaat tttttgcaat agtggatgag attgttctaa atcttcaaat acacgttcaa
    15481 atacagtagc tggcactaat tcttcgaccc ctgcaaaacc ttcgtttttc actacttcgt
    15541 tatagaattt tgtttcttgt gtagttaata cacgctgacc acggttcatt aatactaatt
    15601 gatcttgatt ttttgctgtt gcttcttcta aaattttatc ctgaatttcc ttagataagc
    15661 ttaccatagc tgcgctaaaa gattcttcgt taccatcttt aaaagctttc atcaattggt
    15721 cgcttgcagc tgttacacct tttaaatttt taactgtcat tattttacat ctccttgtcc
    15781 aaatgtttta tttaatgctg ctgtaaatgc agcaattttt tctgctcttt tttctttaac
    15841 gtcattcaaa atttcttcaa cgctttgttc ttttttagct tcagtacctg agctattttc
    15901 tgcatcgata atttcatcga ccaatccata actcaaagct gtttctgcat ccataaacga
    15961 ttctttttca agaagttctt gcaatgcttc atctgtgcca ttgaatcgtg ttttatatga
    16021 agcctttacc gatttatcaa ttgattccag ttggtcagca atcgtacgga agtcatcgac
    16081 atttccttct ccgtatgtgg aagcgcggtg aatcatcaat tgtgcattgt tgtagatttt
    16141 tatagtatcg ccagccattg cgataattga agcagcacta gcggctaagc cgttaatcac
    16201 aacgttaact tttgctttat ttgacttaag taagttccca atagcaatcc cttgaaatac
    16261 gtctccaccg tttgaattaa ttactacttc aatttcttct tgatcaccta gactatccaa
    16321 aatatttttg attcccttgt cagtattccc ttcaaagaac caactagaac caataaatcc
    16381 ctgaataaaa atttgcggta ctgcgccttc attctttact gctagaaatg ttttcattgt
    16441 cgtcattcgc ctcacctcct ttcgatactt gttgattgtt tttagttata aatatttcat
    16501 ctgccatcgc cttatcagag cgatcatttc caacgcgttc tcttccttcg ttgattgtaa
    16561 atactccatt tctaatgcct acatcaatag cgtcaaccaa atctttgaag ctagtaatct
    16621 tgatcatagt tgtatccaca cgtacaaaat tccctgacaa gtattcttct acttcataga
    16681 gactagcgtt aaacgcatcc tgaataagtt cagcaatcgg tatgatttcg aacattaaaa
    16741 aagcgtccac ttgatccgat aacccactca tgtctccctt tagtaggttt ttcggaacgt
    16801 gaaacgctgc tgctgtcatc tcaaagatgt cgtctattaa gttttttata tctcttgaat
    16861 tgctttggaa gtttccgctg aaatcttcta atgtgtactc attttgtaat tgaaataccg
    16921 cacctgcatt atcagcttcc ataaaagcct taaattgtga tgtcatcatt ttattgattt
    16981 gatcttgtgt tgtattgtct tgcggtcgga ataaattccc tttcagtacg tatctacgag
    17041 cgttagagcg cttgtaaaca ttcatggcac tagaaatgag tttcccatac gcttgataat
    17101 acgcatcgac tagttgccta atttgttgat ctgcgtattt tatatagata acatcacttt
    17161 ctagaaattc tctatcaagg actatgttgt taatttgcac ttgagaaaac acatcatctt
    17221 tcaatgcata ttctgtgaca tcccaactat ccgcaataaa tatttcgcta gaattattag
    17281 acggagaaac gatcaatact tcattgtaga atattaatct cctgatcagt ttttttctaa
    17341 attctgttgc attatttttc ttattaggag ctacattcag cctatagtaa agatcattct
    17401 ttttattttt tccatcttca tatgacttga attccgcttt actcatcgca tttgcaatca
    17461 aatcaataca agtttcaatc gcaaattttc gatacacaaa atcaacttgc aatttacaaa
    17521 agtattcttc taaaggaacc gttgcttttt ttgtgaagta tcctaccgcc ttttgaaaaa
    17581 tccccacttt ctcacctcct ttcaagttag aatactagag gagtaaatcc agttcctgta
    17641 ttttctactg agctatttgt gactgttaca ggagcagaat cataaatatc atctaaaaaa
    17701 ttcaaaccat gaaggaatga aaaaaagcca tccgtttttc tagtttcagg ttctattttt
    17761 tcatagcgta tatttccatt agaaatatgc tcttcatata cattcatgca ataccaacgc
    17821 ataatcgcat cgtcaccaaa aaataaacgt tgattaataa aaaggtcatc aaccagatct
    17881 tttaacatac catgtgtaac agatccgctt cgaacaattt ccacagtaaa acctgcttct
    17941 tctaaagcgg gcttcaatat ttttgcacgg tacatatcca tagcgatttt tttaatataa
    18001 tatttattac tcatttcaag aaaccaacct acaatataat cagcttctat attttttcca
    18061 tgaacgatct gtgattttcc ttgatctata gaaatatcta taacctctcg tttgatgttt
    18121 tgtaatcgaa gggctgattc gtggataaaa gtatgttgtg taaaataaac atctttatcg
    18181 tattttccta gcaacccaac gctggcaaaa tctcgtctat cagcaaaatc gactgttcct
    18241 atcacttcat ccattttttc aggaaattct ttttctttcg tatgcagaac atcatcatat
    18301 gaagcaacag caaatcgtgt atcttccata ggtctgttca ttcgtttggt catgaacgta
    18361 agtcttaaac cagcattacg ttgcatttga gagtattctt gaaacatttt ccgttttaaa
    18421 tctgcattgt aattaatagt tggacaagct ttttcccaca tgtcgggatc atcaacttca
    18481 ttatcgttat ccaagcgaca aataaatgga aacaaactag aaaattctgc tccatccttg
    18541 tcaattccaa gttctccaga aagaatcatt tttgattctt ctataatgtc atcaagcgga
    18601 ccaccacgaa catgaccatt agttgtatca taaaattctc tataatctcg aattttacca
    18661 ccaccagaag tagccacatt tatcattgaa taatcttcat tttcgtgaat ttcatcaaag
    18721 cggtttgcac ctggtcgctt cccatctttt gttctagcat ttgccgtgtt ataacgaagt
    18781 ttgctgtttg tagcgatatt ttgaataact tccttcgtag ctttaaatac ttttttatct
    18841 aaatcaggat gatctttaat tactttaaat acatcatcaa aactagtctt tgcttggctt
    18901 tcattattgg catagatatc aatatcataa tttttaatac cgtgtttagc ggttagtaga
    18961 aagaagttgt tccaagaagc aaaaccagtt ttaccattac cacgtcccat taatgaaaga
    19021 tatctattga acactagtgt tttatctttt ttccatcgaa caccataaat aaaacattgt
    19081 agaaattttt cccacggaat taattcgaat ggaaagtatt gtgctggtat attgattgaa
    19141 tcctctacca tctgcttatc gaagtaaata tcttctctag taaagactct ttcttctaga
    19201 taatttttta gcaataattg ctctttgcat accttgatag tgccttcttc tatagctttg
    19261 aaccaatttt caatatgctt ataactcagg aattgattca tttgcttcac ctaccaattc
    19321 aggagtaatg gcaagtttat ccaacatcaa tcccatttgt ttgttgacag aaacaagcaa
    19381 cgctactgat tcattctttt taccattctc cagtctaatg ccgttctcgg atatatcttc
    19441 ttccagtgat atcgccgttt cccataaact gatataacga tcaacattat ctaagaatgg
    19501 ctcaatattt gttttctgac tttccaattg gcttattaaa gagcggcgta atttttctct
    19561 gtagcgattt tgagacaatt cgtttttaaa cattttagcc ctcctttcat gataaagttc
    19621 gaaaaaatct cttttcctga cagccccctc cgtttcatca cccccaaaaa atttgcgatt
    19681 tattttaagg gggggttatc tcaccatcgg aatgaaagct tcagcgaagt caatgtaata
    19741 attaatctct tcaatgctat atccaaaaat attttttatt ctttcaacgt tattatcttt
    19801 attcaacgct tctcttactt gattcacttt gtatttactg caacagttat ctgataacag
    19861 atccctaata cctacatagc gaacgtatat caaacgttta attaatcctt gagtataaga
    19921 tgaatactct tcaatctttt ctgtgtcata ctctctgcca ttgtcattga tgatcatgca
    19981 cttaccacct ttcacttgca tcgaagttag caaagctttc tatcttcttc tcttgtttat
    20041 ctaatgctgt aagatatctg ccatgaactt cattatgatg ttcaacacat aaacaaataa
    20101 gattatctaa atctaaagct aagtcaggtc tatccttgac ttcctttata tgatgaacgt
    20161 tctctactct atgatactta cctagtcttc tacactcttg gcattcatag tgatctcgtt
    20221 tcatcgcttt ctctctaagc ctgcgccatt taggagactg atagaactta accaaacaat
    20281 cttctcttat caactgtaat aaccatctat agaattcctc ggtcatgtcc catctccttt
    20341 cgcaatctta tttaatactt agctattctt ttgccataca atggaataac ttcattgctt
    20401 tcctttcgtt tatatgtatt gctctttatt ggtcttctat actttcgtac tatctcaccg
    20461 ttaccgtttt gcacagtgat tacttcatgc ttctgttcta agtattgtgg tctatacatt
    20521 gttgttacct cctttttgca aaataaaaag accactcaac gagtgatctt aaaaaattac
    20581 taattttaaa ttactttagt actgattgca gctataactg atgtcagggt actaaagtct
    20641 cttccatcca ttgtctgaaa ggacatgaag tcaggttcta gtatatacaa tatgatatct
    20701 tccatatcta attttcgtga ttgacacatt gttgttaaaa aaagataatt gggatgcttt
    20761 tttacataat tttttataat tgataaatac tcaattagct gatcaacctg taatcttctt
    20821 tcattagaaa caaaaaaaac ttcaggcata acaaaagtta tatttatgtc atcaaaattc
    20881 gctttaattt ctgctcttgg aacgtcattt tcaaatatta aaacttcctt aattatggca
    20941 gaagtgcgta ggttttggcc actatctttt tctgataatt ggtgaggtaa tctctcataa
    21001 aatggaggtt cttttacttt gacttctccg ttccctgtat ccttcttcac tacttctcca
    21061 tcaggatcgt atttgacttt tataccttgg tatgtctcag ttccttcagt tatttctttt
    21121 ttttgagtag ttttcttgtg ggaatttttt gcttttggct ttggttttgg aggattcaaa
    21181 tagtctctta aagctttcat tgattctttt tttcttctaa gctttcctcc atcagttaat
    21241 cctccattta tttcactata ttcagttata gactttactg gtttaatctc atcttcaaaa
    21301 tatgcacaat caatgttatg ttcgtcagat ctgtgtttac ttaaataatt aaccccagta
    21361 cttcttctat taaagactaa tcttgcttca cagctaggat cgggacagta aatgtgttgt
    21421 acaatctcca aaaagtcttt ccttcttgga atttcttcta aactaattat ttctttttta
    21481 ccctttacca caaaagcagc ttcatcaaat ttcaaaaaat cacctttttc actattatta
    21541 aaacaaaaaa tctgttaata taatagtcgc caaacaatta taatcaaaca gtttatccaa
    21601 gtttttcaag ctcggatttt tttgtttgat ttaattatat tgctatgtga atatttaagc
    21661 aatgtttttt taaaacgatt ataattggta attttctata gcctctttta tagttttagt
    21721 tggaatttta gttattttgg tcccctctct aacacaataa taatggggag ctaaaattcc
    21781 attctcatcc tcttcaaaat cgaaagcttc ttttcttttt ttattataaa agcagtaaac
    21841 tttttcacaa ggaatatcta ctaactccca ttcctctaaa tacattttct catttttcat
    21901 tgaataaatt atattattta aat
//
LOCUS       DACECB010000051        21842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECB010000051 DACECB010000000
VERSION     DACECB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.051"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO04_001932"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO04_001932"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0213533.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO04_001933"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO04_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0213534.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO04_001934"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO04_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0213535.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO04_001935"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO04_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0213536.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO04_001936"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO04_001936"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0213537.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO04_001937"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO04_001937"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0213538.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWO04_001938"
     CDS             complement(8052..8522)
                     /locus_tag="IWO04_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0213539.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWO04_001939"
     CDS             complement(8568..8993)
                     /locus_tag="IWO04_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0213540.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWO04_001940"
     CDS             complement(9060..9329)
                     /locus_tag="IWO04_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0213541.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWO04_001941"
     CDS             complement(9340..10935)
                     /locus_tag="IWO04_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0213542.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO04_001942"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO04_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0213543.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWO04_001943"
     CDS             complement(14488..15048)
                     /locus_tag="IWO04_001943"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0213544.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IWO04_001944"
     CDS             15401..16375
                     /locus_tag="IWO04_001944"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0213545.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IWO04_001945"
     CDS             16545..17945
                     /locus_tag="IWO04_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0213546.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IWO04_001946"
     CDS             complement(18023..18745)
                     /locus_tag="IWO04_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0213547.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IWO04_001947"
     CDS             complement(18824..19330)
                     /locus_tag="IWO04_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0213548.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IWO04_001948"
     CDS             19453..21252
                     /locus_tag="IWO04_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0213549.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..>21842)
                     /locus_tag="IWO04_001949"
     CDS             complement(21320..>21842)
                     /locus_tag="IWO04_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0213550.1"
                     /translation="ESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQP
                     KPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDI
                     GGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVG
                     SVQWFAPDFAVSM"
BASE COUNT         6051 a         4790 c         3966 g         7035 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 ca
//
LOCUS       DACECB010000052        21534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECB010000052 DACECB010000000
VERSION     DACECB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.052"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..917
                     /locus_tag="IWO04_001950"
     CDS             51..917
                     /locus_tag="IWO04_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0213551.1"
                     /translation="MAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
     gene            complement(1341..1511)
                     /locus_tag="IWO04_001951"
     CDS             complement(1341..1511)
                     /locus_tag="IWO04_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0213552.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1705..1884)
                     /locus_tag="IWO04_001952"
     CDS             complement(1705..1884)
                     /locus_tag="IWO04_001952"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213553.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2271..3383
                     /locus_tag="IWO04_001953"
     CDS             2271..3383
                     /locus_tag="IWO04_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0213554.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3461..3754)
                     /locus_tag="IWO04_001954"
     CDS             complement(3461..3754)
                     /locus_tag="IWO04_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0213555.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3922..4236
                     /locus_tag="IWO04_001955"
     CDS             3922..4236
                     /locus_tag="IWO04_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0213556.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4257..6236
                     /locus_tag="IWO04_001956"
     CDS             4257..6236
                     /locus_tag="IWO04_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0213557.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6249..6575
                     /locus_tag="IWO04_001957"
     CDS             6249..6575
                     /locus_tag="IWO04_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0213558.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6591..7109
                     /locus_tag="IWO04_001958"
     CDS             6591..7109
                     /locus_tag="IWO04_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213559.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7109..8461
                     /gene="celB"
                     /locus_tag="IWO04_001959"
     CDS             7109..8461
                     /gene="celB"
                     /locus_tag="IWO04_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0213560.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8545..10050)
                     /locus_tag="IWO04_001960"
     CDS             complement(8545..10050)
                     /locus_tag="IWO04_001960"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0213561.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10142..10732)
                     /locus_tag="IWO04_001961"
     CDS             complement(10142..10732)
                     /locus_tag="IWO04_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0213562.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10911..12557
                     /locus_tag="IWO04_001962"
     CDS             10911..12557
                     /locus_tag="IWO04_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0213563.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12606..13325
                     /locus_tag="IWO04_001963"
     CDS             12606..13325
                     /locus_tag="IWO04_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0213564.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13804..14751
                     /locus_tag="IWO04_001964"
     CDS             13804..14751
                     /locus_tag="IWO04_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213565.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14763..15683
                     /locus_tag="IWO04_001965"
     CDS             14763..15683
                     /locus_tag="IWO04_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213566.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15724..17181
                     /locus_tag="IWO04_001966"
     CDS             15724..17181
                     /locus_tag="IWO04_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213567.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17526..18140
                     /locus_tag="IWO04_001967"
     CDS             17526..18140
                     /locus_tag="IWO04_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0213568.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18133..19857
                     /locus_tag="IWO04_001968"
     CDS             18133..19857
                     /locus_tag="IWO04_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0213569.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19854..21305
                     /locus_tag="IWO04_001969"
     CDS             19854..21305
                     /locus_tag="IWO04_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213570.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6950 a         3657 c         4359 g         6568 t
ORIGIN      
        1 gagaaggaga aacgctacaa accggtctaa ctacaggaga agatggaaaa ctggcaattg
       61 acggtttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      121 tagatgcgac accgatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga
      181 cgaaagcaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg
      241 gggaagtcct gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt
      301 caggtctaac cacaggagca gatggaaaac tggcgattga cggcttagca ccaggagtgt
      361 atcagctggt agaaacccaa gcgccaacgg ggtatgaatt ggatgcgaca ccaatcgagt
      421 ttgagatcga aagaagtcag acagcggtgg tagaattgac gaaagaaaat cgacttacac
      481 caggcggtgt cgtgttgaca aagatcgatg accaaagcgg agaagtcctg caaggagctg
      541 tttttgagtt acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag
      601 atggaaaact ggcgatagat ggcttagcac caggagcata tcagctggta gaaactcaag
      661 cgccaacggg gtatgaattg gatgcgacac cagtaacttt caagatcgaa aaagaacaag
      721 aagtggcgat ctttttaaca aaagagaata gaaaagttgc tgatagctca gatataagag
      781 gaaacaatcc aacaagcagt ggaaataagc atcttccaaa aactggagaa accatcatta
      841 gccagtttat attgtcaata ttgggtgtgc tacttgtttt catcagtata aaatttagaa
      901 agaaacgaaa tatatgagct aaaataaaaa attgaataag catagttttt aaaatcaact
      961 agtacccatc aattgaatgg aaactaccga ttttagaagg ggtatacaaa caaatattag
     1021 tatcggagta gagaaggttg aacccaacca gtaaataagt aaagacaatt ttgtgctgcg
     1081 ttgagaagtg aaaaataaac ctttgtagta atactagtta ataaaaaata tacaaaaaca
     1141 ctgcgaaaat cattcttgag tttcgcagtg ttttttgcct aatgactcaa gtttattggg
     1201 taatactttg attttctttc tacaaataat cagcgatcag ttgtaatttt ttctctagtt
     1261 ttaatggctc tttgtcaata aggagaaaaa caagtcgggt cttttgtctc ggaaagcttc
     1321 aaccaactcg tgaataaagg ttacgcctcc ttgaaaggcg tggaaaaacc aagtaatcga
     1381 tcaatcacca atttcgcatt tggtagaaca tgtttggtga gctgaaagta ggtggcgttc
     1441 atgtctgtaa ctaagaattc tatttcttta ggattagtgc agctaaggaa atagtttgtt
     1501 aatcgaggaa ttttttgtgt aggtaaattt tctcgattaa tttttctgtc tcgccatccg
     1561 cggaaataaa actcagttta tcctcaacgg aaacgtgaga acgaaattca caaccctcaa
     1621 cactcaagac agaattcttt tggaagactt tggtaaatag cttctcaact ctttcaatgt
     1681 gcgaataacg gttgtcaaag atacctaaca acttttcgaa ataaaaaata acgacacttt
     1741 ttcagtcagt aaagaagtga ttttatgtct gacatgattt gaaatcgagt gccttggacg
     1801 gataaaataa ctttgagcgt tccaatggct tcgacagttt ttacaggtat agcgctgctt
     1861 ttttaggcgt ataaccattg gcatatgatt atattattca aagtggacaa tggtttcctt
     1921 cttcttgttt ttcaccacca ttaatttcct atttccatct accacagcag aaccacaatt
     1981 tctacagaca catggagcag ggaagagaac tgcagcaacg atcaatgttt ttttcttctg
     2041 taaggtttcg taagagacct ctgtaatcat caaatttttg tctattgatc tcagaatttt
     2101 tttgatagaa ccattcataa agcatatcgt tctctcagtt gtttattttg tggtgattta
     2161 atcatacaag aaaacgatat gtttttttat aaaaacatga ataggaaaaa tagttgacaa
     2221 gaaaatgaca acgccatatg atgttccata gtatttacaa cctgtcatag atgactgtaa
     2281 aaaataaaag taaaaagaaa ggacgacaac aggtggattt ttatcagtct ctacaactag
     2341 atccgtttat tttaaaacaa aaaatacgag aagcagcttc taaaaaagaa aagtgcatgt
     2401 atatttgttc attgttttta cgatcattat tcatcgtatt gtttgccatt tgttttatca
     2461 ttataattac aacattattt gaatcgaagc ataaaccata cgctgtggtt ttattctgta
     2521 tgttgatgag tattcgtttt gttgactttg gttataagat ttcccattcg atcataggtt
     2581 tagcaatcgt catgttctca ctattagtag caccgtatgt ccagctgatc aaatggtcag
     2641 ttatgggcat gttgattcat tttattttac taagcagcat attaatggca accgctagtg
     2701 atcccaaaat ggggaatgct tctttgtacg gcttttcata tctctttatt gtttattcgt
     2761 tacctaaaga ttcgttgaac aagaatttct ttacacaaac aagtagctta ttatttttgt
     2821 ttttttgttg gttttcagtt ctcctttaca gaaaacatcg agaaaagaac aaagacaaaa
     2881 gcttgtttaa agagattttt ttgaaaggca tgtattcgca ggaaaaaatt tggatgctca
     2941 tctatgcatt tggcattagt cttttgatcg ttgcaggaga gtatgtccct tttcagcgat
     3001 tgatgtgggc agggtttgca ttttcttcta ttgtatctag ctatggattg atgtcgattg
     3061 gtttcaaaga acgagcagta gaccgaatca ttggctcatt gattggatgt gttttattta
     3121 ttggtatcag ccaatttatt ccttttaact gggtcggtat attaggtggt ctagctttag
     3181 gtgtttgttc tacctataga tataaaacag tttttaattc ttttggtgct ttggcaatca
     3241 ctgccagttt attcggggtt ccaggagcag taacgatccg catttttgag aatatccttg
     3301 gggtatgttt aggaatcatg tatattgggg tgactgaaat acttatccga aaaataagag
     3361 aaaaacacgg tttgaatcat tagatgagat cgaataaaaa ataaggaaaa agtgaagata
     3421 cacatggatg ataaattgct atcattccat gtgtatcttt ttacggtaaa tcagtgaaac
     3481 gttcgacttt catttttaat ttatactttt tacggagttc gtttatttga gccatcattt
     3541 cggatttgtg atgcttttcg attgcttctt catctttcca acggtcgatc agtaaaagag
     3601 attctggatc ttctagtgga aagtaatagt catatttctc gtttccttct tctgcacgaa
     3661 tcgctttgac aatgtttctt tcgaccattt cttctgcaaa ttttctggct tgtccatttg
     3721 tacttgtata caaaatattc actgtgatac tcactaaatc ccctcctgaa aaaattctat
     3781 cactagtata tcacggtgcc agaaattatt attcaagata tgatacacat tttttccgca
     3841 atgctacgga aatatatatt ttgaatagaa aacgcttaca agatatgctt ggtttataaa
     3901 aataaaagga gtgattgatt tatgaaaaaa gcattgatta tttgtgcggc aggaatgtca
     3961 tcctcgatga tggcctcaaa gacaacggat ttctttaagc aaaaaagaga agaggtactc
     4021 gtagatgcag tttcagctac agaaggcgat aatatgatta agaatagtga ttttgattta
     4081 tttctgatca gcccgcaaac gacgatgttt ttggataaat tcaaaaaatt aggagaatct
     4141 gttggaaagc ctgttgttag tatccctttc caagcgtatg tcccaatccc tacaggcatc
     4201 cagaagttag cagaggtgat cgaagagaac atatgatgga ataggagtgg atgataatga
     4261 aacagaagga aaaattgctg ttgagatatt tgattgatca caagaaggag tatgtcacca
     4321 gtcaacgttt agcttctgaa ttgttattat ctgatcggac aatccgtaat tacttgcagc
     4381 ggatcaaaga gttagtagaa aaaaacggag gtaagatcat tgctaaacca ggttatggct
     4441 accaactgca tattttacaa agactgacat ttgacttgtt tttgagtcgg caagaaatag
     4501 tacctggata tgctagagaa ccgcaaggat tttatgagtc cgaagatcga caaaagtata
     4561 tattgaacca attgcttttg gaagatgcca ttctttttat ggatgatttg gcagaagagc
     4621 tttatatcag tcgttccagt ttgacaaagg acatgcaaga aatcaaggag cgattgattc
     4681 catattcact aaaaatcgtt tctaagcatg gtcaagggac atggatcgaa ggggaagaaa
     4741 gaaatcgacg gcattttatc atggatactt tttttggaaa ccattatgga aattcattga
     4801 aagaatattt agggcaaagc cgttttttcc cagacatcag ttttgaagag ctagtcatta
     4861 tcattctcga tgaaacaaga gaagctaagt taaaagtttc ggattttatc atacagaatc
     4921 tgactttaca cttggctttg gggatcaaac ggctccgaga agggtttgaa atcaaggaat
     4981 taggcatcga acaagaagtt tctaagcgag tagagtatca agtggcgcag cggatcgttc
     5041 aacgtattga ggtaatcgca ggtgtatcgt ttcccgaaga agaaattgct tatttgacat
     5101 tacatctcat ggctaagtcg aatcacaaag aaaatcaaaa tgatcaggaa ctatcaaagg
     5161 aattagcgaa agttctgaaa agattgtctc aggaatttca tttttcatta gtagaggatc
     5221 tccagctttt caatggacta ctcgatcatt tgtcaccgat gatgattcga ttgagccgag
     5281 gtattcgcat tgagaaccca ttgactgagg agataaagaa agaaaatccc aaagtctttg
     5341 acgcagtaaa aaggcatttc agcaacatgc cggctttgaa aaactatacc atcaatgaag
     5401 atgaatgggc ctatttggct ttgcatctca tggcggcatt ggaaaaagaa agagctgcgc
     5461 ataaactaca tgcattgatt atctgtgcaa cgggttacgg cagtgcccaa ctattaaaga
     5521 atcgcgtagt cagtgaattc ggtaaaaata ttacagtggt aagtgtaaaa ggctattatg
     5581 agatcaatga aacaacttta aaaggaatcg acctgattat ttcttctatt gatctttcga
     5641 caatgttttt caaaattcca gttttacatg tcagtatttt tttgaatgaa gatgatgttc
     5701 gtaaaatacg aaaggtagtt ggagaatcta taccctatca ttcctcagat agaccgatac
     5761 cagcattttc tctacagcaa aaaaagcaga tttacaccga acagttgtct gaaaaatttt
     5821 tcaagactta tacgtacgaa ccgaaaaagg aagaaattct tcatgatttg ctgcaaaagc
     5881 tgagcataaa tgaagaagaa ggatatataa gagaaatgcg aaagcagatc cagcaacgtg
     5941 aaaatatggg acaaatcata ttcagtgata cagtggcagt cccacatccc gctgttccag
     6001 ttggaatcga aacaaaggta gccgttgcat tgattccttc tgggatgcag tgggatcaat
     6061 atcaagaaat tcattttgtt tttttagttt cgccttctta tgtcgaaaat gaaggaatta
     6121 cggtcgtgac taaagcaatc gttaggttag ttgaccagct aaaagtacag caggaaatct
     6181 tagctgaacc tatttttgag aatttcagca gtaaatttat caaactgatc gaataaggag
     6241 gaaaacagat ggctgaaaaa ataaacagtg aaacactaca agtgacttct tttgatatca
     6301 ttttccatag cggaaatgca cggacactga tacatgaagc atttcgctcg atgcgtaaag
     6361 agatgtttga agaagtagaa gagaagttgc gacaagcaaa tgaagagatt cttcatgcgc
     6421 atcagtcaca aactcaactt ctgaaagaat atgccagcgg gacaaaaata gaaatggaaa
     6481 ttatcatggt ccatgcacaa gatcatctga tgacgacgat gacattattg gaagttgcac
     6541 aggaaatgaa ttatttgtat caaaaaaata aatagaaagg aaggatcatt gtgcaggata
     6601 cagtaaaaag gaaaatcgaa ttagaaaaaa agcaacttag tttgaaaaac atgtatttca
     6661 atcgttatct aatcgtacgt tacctgactg cctttttctt tttcatgaac atgcaatgga
     6721 tgattctgct tctatcagct aaaagtttag gaagtagttt accgatgatt ctgattttag
     6781 ctattttgcc tgcagtagga gaacaagtaa agctttatcg aaaacaccaa acgaacgttc
     6841 catggacaaa gtggtatttt ttgttccaga gtgtctttaa tttattagtg attccagtgt
     6901 tgtttacctc aggatttaca ttcttgtatc cttttatggc aaataacaat ggaggacagt
     6961 tatttgtttt cattcttgtt gtaagcggta tttttgccag tgcatttata cagtatcgtt
     7021 taaagaaaat ttcactcaac caagaccaac agtatatccg tattaaacaa tatgaaaaag
     7081 cgttatattt aggaaaggag aataactaat gtttgatttt ttgcagaaat acctgatggg
     7141 acctatgggg aaaattgcgc aacttaaaat cgttcgggct gtcatggcag cggggatggc
     7201 ttctattcct ttcacgattg taggctcgat gtttttggtt ttaaatattt tgccattacc
     7261 gttcccattt ttagaaggat tttttaatgc aacttttttc aaagttagtg acttgtacat
     7321 gatcgtcaat acaatgacga tggggatttt atctatttac tttgccattg tgtttgctta
     7381 tgaattaacg tcaattgaac gagatgaaca aaaattaaat gttaatccgc tcacgggggc
     7441 attactatct gtttttgcat tctttatgtg tatccctgaa ttgatcatga ctgatggtaa
     7501 aatcagcttg attgcttcta tcaatgagac agaaactgta gtaaatggta ttaggatggg
     7561 ctcttttgtc gaacgtttag gaacttcagg aatctttaca gctattatta tgtcgattat
     7621 tgcggtcgaa ttgtattgta tgtgtgtcaa gagaaattgg gtcataaaaa tgccagatgt
     7681 tgttccgcca ggagtttctc gttctttcac tgctctgatc ccaacatttg tcattgcgtt
     7741 tgtggtgatg atcatcaatg gattattagt tgcactagga acggatatct tcaaaatgat
     7801 tgctatccca tttgggtttg ttacgcagtt gacgaataca tggcttggtg tcatggtgat
     7861 ttatttcttg atccatgcat tgtggatcgt agggatccat ggagcgaata tcatcacttc
     7921 tttcttgacc ccgatcgtat tagctaatat ggcagctaac gcacaaggcg caaattatcc
     7981 gttggcagga gaattcaaca attcttatgt aaccgttggt ggatcaggtg cgactttagg
     8041 attgatcatc tttattgcat tcctcgcgaa atctgatcag ctgaaagtgt taggaaaagc
     8101 ctcacttgta ccaggattat tcaatatcaa tgaaccaatc attttcggga tgccaatcgt
     8161 ctacaatccc tatttagcgg ttccgttttt cttagctccg atggcttctg ctacactcgc
     8221 ttattttgcc attcggctgg aaatcgtcaa accgatgctt gcacagatgc cgtggccttc
     8281 accagtcggg atcggagcat ttgttggtag tggtggtgat tggaaagctg ctgtgttagc
     8341 agtcatttgt gcaatcatcg cattcattat ctggttccca tttatcaaat tctatgataa
     8401 taaattatta aatgaagaaa gagaaaaggc agcagaatta gctagtgaaa agttaaaatg
     8461 atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc cctttttttc ttagaaatgg
     8521 gaaaccacta gtatataaat accattattc ttcaatcaac cgcttcacga ttgcataatc
     8581 cccttcataa ggttcatcta caccattgat tttttgatag ggatccacgc cttttcccgc
     8641 aataacgact gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg
     8701 atccatttca taatgaacga tcaaatctgg attagtaatt gcttcattga tttcttccgc
     8761 gatttttttc ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt
     8821 ttctgatagc acttttccaa aatcatgtcg acgggagatt gctttgtttc ctgggctgcc
     8881 taatacaaca attacacggc catctggatg ttcatttttg gcaaatttca gcaatgtttt
     8941 caagcttaga taattatgtg catagtccac atagacatgc gctccattgg attggatcaa
     9001 ttgatccatt cggcctggta cacgagcttc ttttaatccg tcttgtgcat cttgactgga
     9061 ggcacctgtt agtgctgcgg caatgatcgc actggctgca ttttcttggt tgaatcctcc
     9121 agccagcaaa atatcataag cagtatcgca gatagctaag cgatcttcat gagaagataa
     9181 agtaaaaccg tgagtacctt tatctgaacg gatcacttga taattgcttt cttccgaacg
     9241 accataagtg atcgttggaa tctggaatag atcggctgtt tccctaagta aatcaaaata
     9301 gtcactttca tgatttaaaa caactgtttt tgaattcaag atcaattgtc gcttgcaata
     9361 gaaatagtca tcaaatgtcg gatgttcgat cggactgata tggtcaggcg agatattcaa
     9421 gaagattccg acatctaaag tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga
     9481 aacttccatc actagatgag tcataccatt ttcaactgct tctgacatca tacgatataa
     9541 atctaatgac tcaggcgttg tcaaatggga tttgaaatat gttttgccat ctaacgttgt
     9601 attcatagta gaaagcaatg ctgttttttt atttgttgtg tgatccaaga ttgcttttgt
     9661 aaagtacgca gcagttgttt taccttttgt tccagtgaat ccgatcactt tcaatttatt
     9721 ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt gctttacgga tatccgttac
     9781 gatgattcct actgctgaat tttcgtatgg ttgttctgat acataatatt gaataccctg
     9841 ttcgattgct gaatccaaat attcttcttt gaaattcatt cctttacaga aaaacaaagt
     9901 agaagcatca gcttttcttg aatcataact taactgaaga aattcagctt tttctggtac
     9961 atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat agattttctt ttaatagtaa
    10021 atgacggatg tcttctaaag agaagctcat tgattgatcc ctcgctttat tcaatttctt
    10081 ggtttattat agcacactct ttttaatata tcgtttgttt ccgctatgac aaaatccagc
    10141 tttattcaaa ttttcttgac acaggggaca agcggatgag tattttcata aacaatgaac
    10201 tgactgtaat agataggaga acgaatagtg tctccaacaa tccaaaaaca taataattca
    10261 aaaagacgat aagactattg gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt
    10321 tgtggaaaat aatcggcggt atcgttatac cgccatttga ggccccgact gtgtacaatg
    10381 tgtaaacccc aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga
    10441 tcatttccac ttttggaaca atctctaaaa caacaggaaa aagcagactt cccgctacag
    10501 tcttaaaaaa tactgatttt cctaatagga cagcggctag aagcagcatc ataagattga
    10561 tgctccaaac agtaactgag cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg
    10621 cgcccactcc ggcggaagct atatcgtgag ggagcaagaa ccaattcaat ccaactgtga
    10681 taaagaaaag acctaaaaat ataacgggta gtttttttat ttcttgtgtc attgatatct
    10741 cctataaatg ttagccataa aaggctgtat atttctttac tttaccaaaa aaacgaaacc
    10801 gaattcaaaa aaacaattgt tcttataaaa gttatataaa atcgattgcc agtttctgtt
    10861 ttaccaatta aaatgatagt aatgttttgt agaaagttgg aatctgaccg atggataatc
    10921 aaagaaaacc atcattagat caattaacac cgcaagaaaa aatggcacga ggctctgctt
    10981 ggatgaccgc cagtaatatc atttcaagat tgctaggcgc tgtctatatc attccctggt
    11041 atgcttggat gggcgaaaat gctaaggctg ccaatggatt attcaatatg ggatacaata
    11101 tatatgcatt gtttctactg atctctacag caggaatacc tgctgcgatt gcaaaacaaa
    11161 ctgcacgcta taattcattg aatgaatatg gcacttcacg acgattattt atccgtgcat
    11221 tacagatgat gggcgggtta ggcctgttgt ttgctttgtt catgtacatc gcctctcctt
    11281 ggctagctca tgcttctggt ggcggagaag aactgattcc aacgatgcgt tctttgagta
    11341 tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg gtatttgcaa ggcaatcaag
    11401 aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt ttttatatgc
    11461 tattggctac attcatcatc atgaaagtca tggatggtga ctatgtgacg gcggtcacgc
    11521 aatcaacttt cgcggcattt atcggtatgc tagcaagtat tcttgtcctt ttgtattttt
    11581 tgaagaaaca tcaagcgtat acatcggcgt ttattcacta cagcgaaaac aaaataaata
    11641 ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc gattccgttt attattgtgg
    11701 gttcaggggt aacgatcttc aaattagtcg atcagttcac ttttatgaag atcatgtctg
    11761 catccaccga gtattccaat gcacagttac tggatctttt ttcgattttt agtgccaatc
    11821 cagataagtt gacgatggta gttattgcat tggcaacatc gatcgcgtct actgggttac
    11881 cgttgattac agaagctgtt acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg
    11941 gaaatttaca gttgttttca ttttttatgt ttccggcgac atttggtgtt atgttacttg
    12001 cctatccgct aaatacatta ttttatacac cggattctct aggaagtaat gtactgatcc
    12061 aagcaagttt tgtaggactc ttcctaggac tatatatgct agtttccaac atgctgcaag
    12121 gaatgtttga aaacaaggcg gcaattaatt atttggttat tgggtttcta gtgaaactcc
    12181 ttcttcaata cccagccatt cgtttatttg aagtatacgg ccctttattg gcgacaatga
    12241 tcggatttgc tgtgtcttgt accttgatct taaagagaat ccatgaagta gcccgcttta
    12301 aaccaagttt tgtctggcgc aggacattgc tgatctttat tcttaccttg attatgcttt
    12361 tggcttcgtg gatcacaaag atgatttttg gtgctttttt aaacgaagat agcaaattcc
    12421 agtctctatt gttgattctt gtagtagcag gtataggggc tttagtgtat ggttatttag
    12481 ctttgaaaat ccgcttggca gaaagactac taggaccagg aatagcgcgt ttacgacata
    12541 aattaaaaat aaaataagtg aatcagaaga gctagattga tgctcttttg aaatggggga
    12601 aacgcatgcg tttagataag tttttagcag atatgggact tggcagccgc aaagaagtca
    12661 aaggattaat caaaaaagga acgatcgaag tcaatggaca agtggtgaaa agcgataaag
    12721 cgcaagtaaa tgaattcgaa gatgcggtta cttatttagg agaaatcatt acgtatcaaa
    12781 aagattttta ctacattttg cataaacctg caggagtcat ttctgctact caggataatc
    12841 atgatcaaac cgtaatcgat ttattggaag accaggatta tcgggaagat ctttttccag
    12901 tcggccgatt agataaagac acagagggcc tcttgatttt aacgaatgac ggggtgtttg
    12961 cccatcagtt actatcacct aaaaaacatg tggagaaaga atatctagct gaggttagcg
    13021 gtataatgac agaagaagat atccgtttat ttgcagaagg actgattatt gatggcgatg
    13081 agcagacatt gccggcaaaa ctccaaattg atcaagtaga tcaagagaca gaaacttcaa
    13141 ttatccgtct gattttgcat gaaggaaagt ttcaccaagt taagcgaatg gtcaaagcag
    13201 taggaaaaga agtcttatat ttaaaaagaa tccgtatggg aaaattttgg ctgcctaaag
    13261 atttgaataa agggcaatat cgacccatga ccaaagaaga gctagaacaa gtgagagaaa
    13321 aataattgaa tggatagcta aaagaataag cagagtagtt gtagcgccat ctgaaaagca
    13381 gacttgtccg tctgactttc ggatggcttt tttgtgaaat ttcggcttat ttccgtagaa
    13441 gctacttctg gaacaccgtt tgttcggaat aagggactgt gacaagactt ttgttacagt
    13501 ccccattgtt ccttgtgact cagagttaga tagtaaggac acgatctccc taatactcag
    13561 gccaaaacgc tgaattcaca acctctgata aacggtgttc aatcagctga ccctcggtat
    13621 tagctcaaaa aacggcaaaa tatgaggaac tattttcccg tttttttctc taatactcag
    13681 gccaaaacgc tgatttcaca acctctccta tactttgttt cattttccct atactttgtt
    13741 tatttattct cattattgaa agcgctataa ttacgttgcg aatgaatcga aaggggtttt
    13801 tagatgaaaa aacgaaaggg gttcaatcat ttttttatga atctatggcg ttataaagca
    13861 cttgtcctga tggctgtacc gggtatgctt tggatgattt ttttctttta catacctgta
    13921 atggcgaatg tcgtagcatt caaaaatttt catatttcag cagatgggtt cttggctagc
    13981 ttgagggaaa gtccttgggt gggttgggaa aatttcaaat ttttattttc ttcggatcaa
    14041 gcatttctca tcactaaaaa tacaatttta tataatgtag catttatcct tttgaatctg
    14101 ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg actcgttaaa
    14161 gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat cagttacttt
    14221 gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac tggacatgga
    14281 ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt ggtgttcatt
    14341 ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt tatgggaatc
    14401 gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca gcagatcaaa
    14461 catgtgacga taccacaact tattccacta atgacgattt taacgatttt agccgtagga
    14521 aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc cggtgcatta
    14581 tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc cacaggagat
    14641 tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt cttgttgatg
    14701 atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta aggaggaaaa
    14761 aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc tttcaataag acggctaatt
    14821 ttttctctaa tataatgatt gcactctttg ctatttcctg tattttgcca tttatatttg
    14881 ttatcgccat ctcttttacc gatgagtcca tattgtcgac tcacgggtac agtttttggc
    14941 cagaaaagtt cagtatattt ggttatacgt acttgttcga ccagatgcag gataaaattt
    15001 tccaagcgct tttcgtcact gtacttgtta ctgttttagg tacactgatc aatagcacgg
    15061 ctacttcttt atatgcttat gcgatttcac ggtcaaactt tccattccgt cgttttttta
    15121 ccgttttttg tttgattacg atgttgttct caccagggat ggtggccaat tatcttgtta
    15181 tgacaaatct tcttcagtta aaagatacga tttgggcatt gatcttgcca atggctgtca
    15241 gtcccttcaa tattattgtg atgcgcacat tctttaaacg ttcggtctct gattcgatta
    15301 ttgaatcggc acggatcgat ggagctagcg aactgcgaat atttgttcag atcgttttac
    15361 cgttagctgt tccaggtatt gcgacaatca gtttatttgc cgcattgggt tactggaatg
    15421 actggttcaa tgctttacta tacatccaag atgataagct agttccacta cagtatcttt
    15481 tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa ttcaggagca ggaagccaat
    15541 tgacaggcgg attagcctca gttccaggag aatctgccag aatggcgatc gtagtcatct
    15601 cgacgttgcc tattgcgatc agctatccat ttttccagaa gcattttgtc aaaggtctaa
    15661 caatcggcgg agtaaaagaa taaagaaaca aaaatataaa tcataaaatt aggaggaaga
    15721 aaaatgaagt tatggaagaa actagccttt acaggtgttt cagcgttgtt gttgggaaca
    15781 ttagcagcat gcggaggttc tggttctaaa gaccaggcag aagctagcaa ttcggacact
    15841 ttgcagatgt atcaaatcgg agataaacca gataatttcg atcaattgat ggaagtagca
    15901 aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata tcaattacat tggctggggc
    15961 gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg aaaattatga tatcgccttt
    16021 gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg
    16081 cctaagtacg ctgaaaaagc ttataatgat ttagatgaag catatattaa aggaaatctg
    16141 gtcaatggaa aattatatgc tttcccagtc aatggaaatg tctttgctca acaagtactt
    16201 acttttaaca aagctttact agataaatac gatctttcaa ttgatggaat ccaatcttac
    16261 gctgatgcag aaaaagtctt gaaggaattc catgaaaaag aaccaaacac ggcagctttt
    16321 gccattggac aaggcttcaa agtccaaggg aactttgatt atcctttagg gaatacgtta
    16381 ccatttgcta ttgatctgga tggagatgat tcgaaaatca tcaaccaata cgataatgaa
    16441 acgtatatcg gactgttacg tacaatgcat agctggtaca aacaaggact cattccaagt
    16501 gacgctgcaa cgagcaataa ggattatcca ctagaaggaa atacttggct aatgcgtgga
    16561 gaaacacaag gaccgtatga ttatggcgat acaattttga caaatgcagc aaaacaagaa
    16621 cttgtctcaa aagcaattac tgttccacta aaatctacag ctcaagcaca aatggcaaat
    16681 tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag tagaacttct agggctgctt
    16741 aacagtgatc cagaattatt gaatgggctt gtttggggga tcgagggaga agcatgggaa
    16801 aaagtcgatg gttcagatga taaaatcaaa ttgctagatg gttatcagcc aaatacacat
    16861 atgtctgctt ggaatacagg aaacaacaag attctatata cacaagagtc tattacggat
    16921 gacatgattg ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc gattttagga
    16981 ttcagcttca acactgacag tgtcaaaaca gaattaagta atatttctaa tgtcatgaat
    17041 caatatttag atggattgaa tacagggact gttgatccag atgaaacatt accgaaattg
    17101 aaagacgctt tggacaaggc tggctatgat aaggtattga aagaaatgca aaaacaatat
    17161 gatgagttcc gacaagaata ataatgaata aagacaaagg ggttgtgaca aaagacgtgt
    17221 cacaacctct tgttccgaat aaacggtgtt caaaagccag tttctcggta ttaagctaaa
    17281 tatatgtaaa aatgtggaaa gccatattca caaaattttt cttactactc gaaactaagc
    17341 ggctatttca caaactcttg gtaaacggtg ttcaatcagc taaccctcgg tattcgctca
    17401 caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg
    17461 tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag
    17521 aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac
    17581 taaccttgta tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg
    17641 catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa gaaatcacaa
    17701 taaaaagagg ctggaatata tataaaagaa attttttaag aggcaatctg ctatttagtt
    17761 tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt caaatcaaag
    17821 ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta
    17881 cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa
    17941 agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta
    18001 gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg
    18061 gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac
    18121 agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt
    18181 ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt
    18241 agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt
    18301 caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag
    18361 cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat
    18421 cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca
    18481 aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg
    18541 aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag
    18601 gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct
    18661 gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat
    18721 ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac
    18781 acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca
    18841 gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt
    18901 caacaccaag aactgtctga tgattatcgt atattagctg tactagataa aaaaagtgta
    18961 aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg gtatcattat tttagacaat
    19021 atactgctta tctttcttta taaaacattc aaacgatatt cacagcaagt atctatcgtc
    19081 atgcaggcca tggatcaaac agcagcaggg aatttagaga cgcggatcga cataacaaac
    19141 acggaatatg aactgaagga actttcaatc ggtatcaacg aaatgttgga tagcatcaat
    19201 cagtttgtag aagacatcta caaattagaa atcaaacagc aagacgcaca tatgcgggcg
    19261 cttcaagcac agatcaatcc acacttttta tataatacat tagaatatat ccgaatgtat
    19321 gcaatcagcg aaggaagtga agaattagca gatgttgtgt atgcgttttc cgctttgctt
    19381 cgaaacaata cgaatcaaga gaaaacgatc actttaaaag aagaactgga cttttgcgaa
    19441 aaatatgtct atttgtatca aatgcgttat cctaatcgag tagcctatca ttttatgatc
    19501 gatcctgatt tggaaaagat cgaagtacct aaatttgtga tccagccatt agttgaaaat
    19561 tattttaaac acggtattga ttttactcga ttcgataatg cgctaagtgt caaagtcttg
    19621 caggaaggca aacgggtaag gatcatcatc aaagataatg gaaaagggat gactgaaaaa
    19681 cggctaaaac agattgaaga aaaactgtct catcctaaag tggaacttca cggttctatc
    19741 ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg gcagcagtta ttatatgtcg
    19801 cttgagaata atgagacagg tggactgacc gtttctatta cattcaagga gggatgagcg
    19861 tgtatcaagt gttattggta gatgatgaat acatgatttt gaatgggtta aaaaagataa
    19921 ttgattggca aagtttaggc tttcaaatag tagcaacagc agagaatgcc aaagaaggac
    19981 tagctgtact tgaacagcga cagattgatt tagtagtaac ggatgtgaca atgccagaaa
    20041 taaatggact ggaatttatt gaagcggctc aaaaagaacg tcataatttt gaatttatga
    20101 ttttatcagg ataccaagaa ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga
    20161 attatttgat gaaacccgtg aataagttcg aattggttga aagcctcaag aaaatcaaaa
    20221 cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa gaactgttat
    20281 tcagtcaatg gttgaatgaa gaattggatg aaataagtga agaagaaata ataggacaaa
    20341 taagtgagaa gcagcatagg atagtattga ttcaactggc aagaactcat gatactctta
    20401 tcaatagatg gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat tacggagacc
    20461 gtgtattgat cacgttgttg ttggaggcag atgaagttga tggcttctgt tgttttgttc
    20521 aagaaaactt tcctgatcag gaatggctga tcagtatcgg ggaagagaca gattctttgg
    20581 acaaaatccc tgaaacattc caacaagcga aagacaatct acagttgcat caattttatg
    20641 gagataaaga cgagcatgtt ttttacgcag tacaagcaga aacaaaagat caaaccatcg
    20701 atttttcaag tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga
    20761 tcactgattt ttttgaacag tttcagttag cagccatgat gccggaagat atacgatatt
    20821 ccagtttttt actgtttatg gagattcaac gggaattgat tgatctggaa gatgaggaat
    20881 atttacaagg aatcgaaaaa atccatcagg caaaaacggt gcaagaactt cagcagctac
    20941 ttctgtcatt catcaaaaag catcagagac agaaaaagta cagtgacaat gtggaaaaag
    21001 taatcgtaat cctgcaccag cattatcaag aaccacttac actcaaagaa gtgtcagaaa
    21061 acctgcattt aaatgtcatg tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt
    21121 tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta
    21181 atcaaaacat caatgagata gcaagtgaga ttggctacaa caacaccacg tatttctcta
    21241 aattattcaa gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac agtggagaat
    21301 cataaaagaa aaagccaatg taatgaaata acttataaaa aattgtctag aaatctaatt
    21361 ttgggctgtt cttattactt gaggcaaggc agctttttca tgacctctcg ataaacggag
    21421 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttt
    21481 cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacgacctct ccac
//
LOCUS       DACECB010000053        20746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECB010000053 DACECB010000000
VERSION     DACECB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.053"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            167..361
                     /locus_tag="IWO04_001970"
     CDS             167..361
                     /locus_tag="IWO04_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213571.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="IWO04_001971"
     CDS             630..929
                     /locus_tag="IWO04_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213572.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="IWO04_001972"
     CDS             complement(1026..1460)
                     /locus_tag="IWO04_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0213573.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="IWO04_001973"
     CDS             complement(1692..2402)
                     /locus_tag="IWO04_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0213574.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="IWO04_001974"
     CDS             complement(2392..3252)
                     /locus_tag="IWO04_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0213575.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="IWO04_001975"
     CDS             complement(3257..3895)
                     /locus_tag="IWO04_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0213576.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="IWO04_001976"
     CDS             complement(3910..4209)
                     /locus_tag="IWO04_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213577.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="IWO04_001977"
     CDS             complement(4252..5736)
                     /locus_tag="IWO04_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0213578.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="IWO04_001978"
     CDS             complement(5756..6217)
                     /locus_tag="IWO04_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0213579.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IWO04_001979"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IWO04_001979"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0213580.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="IWO04_001980"
     CDS             7520..8290
                     /locus_tag="IWO04_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0213581.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="IWO04_001981"
     CDS             complement(8563..9390)
                     /locus_tag="IWO04_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213582.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="IWO04_001982"
     CDS             9958..11193
                     /locus_tag="IWO04_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0213583.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="IWO04_001983"
     CDS             complement(12192..12452)
                     /locus_tag="IWO04_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0213584.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="IWO04_001984"
     CDS             complement(12479..13780)
                     /locus_tag="IWO04_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213585.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="IWO04_001985"
     CDS             complement(14072..14530)
                     /locus_tag="IWO04_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0213586.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15868)
                     /locus_tag="IWO04_001986"
     CDS             complement(14543..15868)
                     /locus_tag="IWO04_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0213587.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17297)
                     /locus_tag="IWO04_001987"
     CDS             complement(15885..17297)
                     /locus_tag="IWO04_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0213588.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="IWO04_001988"
     CDS             complement(17314..18078)
                     /locus_tag="IWO04_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0213589.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="IWO04_001989"
     CDS             complement(18182..19057)
                     /locus_tag="IWO04_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0213590.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="IWO04_001990"
     tRNA            complement(19312..19396)
                     /locus_tag="IWO04_001990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="IWO04_001991"
     tRNA            complement(19414..19485)
                     /locus_tag="IWO04_001991"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="IWO04_001992"
     tRNA            complement(19495..19567)
                     /locus_tag="IWO04_001992"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="IWO04_001993"
     tRNA            complement(19629..19713)
                     /locus_tag="IWO04_001993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="IWO04_001994"
     tRNA            complement(19734..19804)
                     /locus_tag="IWO04_001994"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="IWO04_001995"
     tRNA            complement(19850..19921)
                     /locus_tag="IWO04_001995"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="IWO04_001996"
     tRNA            complement(19933..20005)
                     /locus_tag="IWO04_001996"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="IWO04_001997"
     tRNA            complement(20008..20081)
                     /locus_tag="IWO04_001997"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="IWO04_001998"
     tRNA            complement(20088..20168)
                     /locus_tag="IWO04_001998"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="IWO04_001999"
     tRNA            complement(20170..20245)
                     /locus_tag="IWO04_001999"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="IWO04_002000"
     tRNA            complement(20281..20353)
                     /locus_tag="IWO04_002000"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="IWO04_002001"
     tRNA            complement(20363..20435)
                     /locus_tag="IWO04_002001"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="IWO04_002002"
     tRNA            complement(20451..20522)
                     /locus_tag="IWO04_002002"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="IWO04_002003"
     tRNA            complement(20537..20626)
                     /locus_tag="IWO04_002003"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
     gene            complement(20666..20739)
                     /locus_tag="IWO04_002004"
     tRNA            complement(20666..20739)
                     /locus_tag="IWO04_002004"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20703..20705),aa:Asn,seq:gtt)
BASE COUNT         6558 a         4344 c         3290 g         6554 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaac ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac gacatcatga
    20701 ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacat
//
LOCUS       DACECB010000054        20263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECB010000054 DACECB010000000
VERSION     DACECB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.054"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            208..2250
                     /locus_tag="IWO04_002005"
     CDS             208..2250
                     /locus_tag="IWO04_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0213591.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="IWO04_002006"
     CDS             2261..2881
                     /locus_tag="IWO04_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0213592.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="IWO04_002007"
     CDS             2893..3351
                     /locus_tag="IWO04_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0213593.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="IWO04_002008"
     CDS             3370..3648
                     /locus_tag="IWO04_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213594.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="IWO04_002009"
     CDS             3673..5040
                     /locus_tag="IWO04_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0213595.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="IWO04_002010"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="IWO04_002010"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0213596.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="IWO04_002011"
     CDS             6227..6718
                     /locus_tag="IWO04_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213597.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="IWO04_002012"
     CDS             7161..8360
                     /locus_tag="IWO04_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0213598.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="IWO04_002013"
     CDS             complement(8855..9613)
                     /locus_tag="IWO04_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0213599.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="IWO04_002014"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="IWO04_002014"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0213600.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="IWO04_002015"
     CDS             10713..12158
                     /locus_tag="IWO04_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0213601.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="IWO04_002016"
     CDS             12186..12641
                     /locus_tag="IWO04_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0213602.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="IWO04_002017"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="IWO04_002017"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0213603.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="IWO04_002018"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="IWO04_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="IWO04_002019"
     CDS             14450..15064
                     /locus_tag="IWO04_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0213604.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="IWO04_002020"
     CDS             15305..15688
                     /locus_tag="IWO04_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0213605.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="IWO04_002021"
     CDS             16051..16560
                     /locus_tag="IWO04_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0213606.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..17310
                     /locus_tag="IWO04_002022"
     CDS             16660..17310
                     /locus_tag="IWO04_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213607.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17761..18699
                     /locus_tag="IWO04_002023"
     CDS             17761..18699
                     /locus_tag="IWO04_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0213608.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18712..19764
                     /locus_tag="IWO04_002024"
     CDS             18712..19764
                     /locus_tag="IWO04_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213609.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19822..>20263
                     /locus_tag="IWO04_002025"
     CDS             19822..>20263
                     /locus_tag="IWO04_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0213610.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6421 a         3222 c         4277 g         6343 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaacaacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgggctt ggataattcc ggttgagtca
    17221 ggagatgcta agaaactgga gagattattg ggcgatgaac taattaataa aaaagtacca
    17281 attttaaatt attattccca tagacaataa aaggctattt ttcatgataa tactagttat
    17341 aactgtaaaa atggaagaaa atctgggaaa gaagttttta aataagtgta ttgttcgatt
    17401 ttaataaata ataagcaata ccatccgtct gtgttgcgca acatcgtact acaatgaata
    17461 ttttttcaca aaaagaaaat catccatcga atgaatggaa ggttttagta tacacgaggt
    17521 taatgtcccc tcttttataa tcagaggagt gaggtttttt ctatgaatgt ttctattttc
    17581 ttattgctag tctagtttct cttacgatga aattgggatt ctattccagt ttttattgat
    17641 ttgctgataa aataacttga atggtggata taaacatgat aaataagaga agaataacaa
    17701 aacaaggttg tgaaatgatt ctcaaataag tataatagaa agaaaaagga ggaagaatca
    17761 atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac
    17821 caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa
    17881 gaacatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt
    17941 cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg
    18001 atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc
    18061 ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa
    18121 aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa
    18181 ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca
    18241 aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt
    18301 cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc
    18361 ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg
    18421 attgtcaatg agtttgccaa agtagcagca gtagagaaga ttgaactaga tgtcccagaa
    18481 gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca
    18541 atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca
    18601 atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag
    18661 cttgtccatg caaaagaaga tagtttgaac gtaaaataaa ggagagattg aatggaaact
    18721 tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc tcgctggtac agcaacagga
    18781 atcattattg gattgattcc taatgcagta ttaggtgcta ttttaaaata ttttagtgcg
    18841 tatccctttg cggtaacgat cgcacagtta gcagttattt ttcaattagc aacacctttg
    18901 atcattggtg gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta
    18961 gccggagctt cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg
    19021 ggtgtttacg ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt
    19081 gctgtcgggc tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca
    19141 ccgattgttg taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca
    19201 gcaattactt ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta
    19261 atgtctatct tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta
    19321 gcaatcggaa tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta
    19381 gctgcaacca cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca
    19441 attgcgattg ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg
    19501 attcttattc cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct
    19561 atttcaggta cgcctgcttc tgctggtttc ggtgtggtcg gacttgttgg tccgttggct
    19621 tctttagatg cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt
    19681 tttgcggcac taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa
    19741 gaagtcttca aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg
    19801 taagtgagat gtcaacaaca tctgacttac gtttttttgc caaaatatgg tttttatttt
    19861 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    19921 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    19981 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    20041 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    20101 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    20161 agctcttgga atctatcgac ttggatccat aatacaggtt ctgggctagg ctatgcaggc
    20221 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaa
//
LOCUS       DACECB010000055        18894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECB010000055 DACECB010000000
VERSION     DACECB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.055"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..250)
                     /locus_tag="IWO04_002026"
     CDS             complement(20..250)
                     /locus_tag="IWO04_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213611.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(686..1252)
                     /locus_tag="IWO04_002027"
     CDS             complement(686..1252)
                     /locus_tag="IWO04_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0213612.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1252..2208)
                     /locus_tag="IWO04_002028"
     CDS             complement(1252..2208)
                     /locus_tag="IWO04_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0213613.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2208..2864)
                     /locus_tag="IWO04_002029"
     CDS             complement(2208..2864)
                     /locus_tag="IWO04_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0213614.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3234..5252
                     /locus_tag="IWO04_002030"
     CDS             3234..5252
                     /locus_tag="IWO04_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0213615.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5679..8093
                     /locus_tag="IWO04_002031"
     CDS             5679..8093
                     /locus_tag="IWO04_002031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0213616.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8441..9994
                     /locus_tag="IWO04_002032"
     CDS             8441..9994
                     /locus_tag="IWO04_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0213617.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10375..12159)
                     /locus_tag="IWO04_002033"
     CDS             complement(10375..12159)
                     /locus_tag="IWO04_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213618.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12174..13895)
                     /locus_tag="IWO04_002034"
     CDS             complement(12174..13895)
                     /locus_tag="IWO04_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213619.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13995..14756
                     /locus_tag="IWO04_002035"
     CDS             13995..14756
                     /locus_tag="IWO04_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0213620.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15723..15991
                     /locus_tag="IWO04_002036"
                     /pseudo
     CDS             15723..15991
                     /locus_tag="IWO04_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16663..>18894
                     /locus_tag="IWO04_002037"
     CDS             16663..>18894
                     /locus_tag="IWO04_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213621.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3352 c         3786 g         5551 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      361 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      421 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      481 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      541 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      601 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      661 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      721 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      781 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      841 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      901 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      961 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
     1021 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
     1081 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1141 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1201 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1261 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1321 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1381 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1441 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1501 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1561 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1621 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1681 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1741 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1801 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1861 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1921 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1981 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     2041 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     2101 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2161 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2221 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2281 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2341 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2401 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2461 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2521 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2581 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2641 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2701 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2761 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2821 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2881 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2941 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     3001 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     3061 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3181 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
     3241 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
     3301 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
     3361 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
     3421 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
     3481 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
     3541 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
     3601 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
     3661 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
     3721 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
     3781 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
     3841 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
     3901 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
     3961 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     4021 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     4081 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     4141 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     4201 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     4261 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     4321 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     4381 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     4441 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     4501 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     4561 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     4621 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     4681 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     4741 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     4801 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     4861 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     4921 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     4981 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     5041 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     5101 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     5161 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     5221 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     5281 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     5341 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     5401 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     5461 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     5521 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     5581 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     5641 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     5701 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     5761 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     5821 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     5881 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     5941 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     6001 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     6061 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     6121 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     6181 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     6241 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     6301 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     6361 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     6421 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     6481 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     6541 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     6601 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     6661 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     6721 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     6781 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     6841 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     6901 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     6961 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     7021 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     7081 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     7141 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     7201 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     7261 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     7321 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     7381 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     7441 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     7501 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     7561 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     7621 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     7681 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     7741 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     7801 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     7861 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     7921 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     7981 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     8041 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     8101 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     8161 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     8221 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     8281 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     8341 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     8401 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     8461 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     8521 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     8581 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     8641 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     8701 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     8761 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     8821 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     8881 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     8941 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     9001 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     9061 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     9121 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     9181 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     9241 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     9301 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     9361 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     9421 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     9481 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     9541 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     9601 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     9661 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     9721 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     9781 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     9841 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     9901 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     9961 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
    10021 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
    10081 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
    10141 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
    10201 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
    10261 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
    10321 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
    10381 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
    10441 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
    10501 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
    10561 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
    10621 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
    10681 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
    10741 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
    10801 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
    10861 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
    10921 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
    10981 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
    11041 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
    11101 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
    11161 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
    11221 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
    11281 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
    11341 ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg
    11401 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
    11461 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
    11521 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
    11581 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
    11641 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
    11701 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
    11761 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
    11821 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
    11881 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
    11941 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
    12001 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
    12061 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
    12121 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
    12181 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
    12241 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
    12301 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
    12361 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
    12421 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
    12481 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
    12541 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
    12601 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
    12661 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
    12721 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
    12781 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
    12841 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
    12901 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
    12961 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    13021 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    13081 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    13141 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    13201 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    13261 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    13321 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    13381 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    13441 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    13501 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    13561 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    13621 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    13681 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    13741 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    13801 acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct
    13861 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    13921 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    13981 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    14041 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    14101 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    14161 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    14221 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    14281 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    14341 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    14401 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    14461 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    14521 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    14581 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    14641 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    14701 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    14761 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    14821 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    14881 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    14941 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    15001 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    15061 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    15121 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    15181 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    15241 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    15301 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    15361 gaaattttaa aaaataaaac acgttattga tggatagtta gtttgttatt tggtaatatt
    15421 atagaagttg actggtagtg atttattttt cgtcgtttta taaaaatgtt attttttgga
    15481 aaaattcaca aatatgtaac gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg
    15541 actaatataa atactattgt catttatgca attttttatg gatagtaatt ttagagggga
    15601 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
    15661 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
    15721 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
    15781 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
    15841 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
    15901 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
    15961 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
    16021 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
    16081 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
    16141 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
    16201 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
    16261 tgaaggttaa ccaatacagg gactcactga tgaaattcta gagtctgacc ttggtatcaa
    16321 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
    16381 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
    16441 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
    16501 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
    16561 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
    16621 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
    16681 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
    16741 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
    16801 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
    16861 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
    16921 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
    16981 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
    17041 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
    17101 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
    17161 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
    17221 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
    17281 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
    17341 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
    17401 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
    17461 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
    17521 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
    17581 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
    17641 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
    17701 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
    17761 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
    17821 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
    17881 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
    17941 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
    18001 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
    18061 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
    18121 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
    18181 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
    18241 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
    18301 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
    18361 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
    18421 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
    18481 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
    18541 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
    18601 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
    18661 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
    18721 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
    18781 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
    18841 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggt
//
LOCUS       DACECB010000056        18880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECB010000056 DACECB010000000
VERSION     DACECB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.056"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWO04_002038"
     CDS             <1..435
                     /locus_tag="IWO04_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0213622.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWO04_002039"
     tRNA            complement(759..842)
                     /locus_tag="IWO04_002039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWO04_002040"
     CDS             1370..1870
                     /locus_tag="IWO04_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0213623.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWO04_002041"
     CDS             complement(2060..2404)
                     /locus_tag="IWO04_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0213624.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWO04_002042"
     CDS             complement(2558..3478)
                     /locus_tag="IWO04_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0213625.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWO04_002043"
     CDS             3665..4156
                     /locus_tag="IWO04_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0213626.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWO04_002044"
     CDS             complement(4180..6555)
                     /locus_tag="IWO04_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0213627.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWO04_002045"
     CDS             6881..7507
                     /locus_tag="IWO04_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0213628.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWO04_002046"
     CDS             7530..8234
                     /locus_tag="IWO04_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0213629.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWO04_002047"
     CDS             8426..9259
                     /locus_tag="IWO04_002047"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0213630.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="IWO04_002048"
     CDS             9456..11384
                     /locus_tag="IWO04_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0213631.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="IWO04_002049"
     CDS             11522..11977
                     /locus_tag="IWO04_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0213632.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="IWO04_002050"
     CDS             12447..12716
                     /locus_tag="IWO04_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213633.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="IWO04_002051"
     CDS             13116..13370
                     /locus_tag="IWO04_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0213634.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IWO04_002052"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IWO04_002052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0213635.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="IWO04_002053"
     CDS             complement(14543..16816)
                     /locus_tag="IWO04_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0213636.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="IWO04_002054"
     CDS             complement(16809..17348)
                     /locus_tag="IWO04_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0213637.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="IWO04_002055"
     CDS             complement(17484..17702)
                     /locus_tag="IWO04_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0213638.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="IWO04_002056"
     CDS             complement(17699..17944)
                     /locus_tag="IWO04_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0213639.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="IWO04_002057"
     CDS             complement(18060..18788)
                     /locus_tag="IWO04_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0213640.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5798 a         3654 c         3316 g         6112 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa
//
LOCUS       DACECB010000057        18438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECB010000057 DACECB010000000
VERSION     DACECB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.057"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..2271
                     /locus_tag="IWO04_002058"
     CDS             82..2271
                     /locus_tag="IWO04_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0213641.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2268..4061
                     /locus_tag="IWO04_002059"
     CDS             2268..4061
                     /locus_tag="IWO04_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0213642.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4261..4575
                     /locus_tag="IWO04_002060"
     CDS             4261..4575
                     /locus_tag="IWO04_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0213643.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4596..4973
                     /locus_tag="IWO04_002061"
     CDS             4596..4973
                     /locus_tag="IWO04_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0213644.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            4983..5756
                     /locus_tag="IWO04_002062"
     CDS             4983..5756
                     /locus_tag="IWO04_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213645.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5778..7181
                     /locus_tag="IWO04_002063"
     CDS             5778..7181
                     /locus_tag="IWO04_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0213646.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7363..8547
                     /locus_tag="IWO04_002064"
     CDS             7363..8547
                     /locus_tag="IWO04_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0213647.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8544..8834
                     /locus_tag="IWO04_002065"
     CDS             8544..8834
                     /locus_tag="IWO04_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213648.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8831..9052
                     /locus_tag="IWO04_002066"
     CDS             8831..9052
                     /locus_tag="IWO04_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213649.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9094..9873
                     /locus_tag="IWO04_002067"
     CDS             9094..9873
                     /locus_tag="IWO04_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0213650.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            9940..10434
                     /locus_tag="IWO04_002068"
     CDS             9940..10434
                     /locus_tag="IWO04_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0213651.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10490..11128
                     /locus_tag="IWO04_002069"
     CDS             10490..11128
                     /locus_tag="IWO04_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213652.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11200..11595
                     /locus_tag="IWO04_002070"
     CDS             11200..11595
                     /locus_tag="IWO04_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0213653.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11579..14032
                     /locus_tag="IWO04_002071"
     CDS             11579..14032
                     /locus_tag="IWO04_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0213654.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14029..16056
                     /locus_tag="IWO04_002072"
     CDS             14029..16056
                     /locus_tag="IWO04_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213655.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16053..17075
                     /locus_tag="IWO04_002073"
     CDS             16053..17075
                     /locus_tag="IWO04_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0213656.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17092..18021
                     /locus_tag="IWO04_002074"
     CDS             17092..18021
                     /locus_tag="IWO04_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0213657.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18266..18382
                     /locus_tag="IWO04_002075"
     CDS             18266..18382
                     /locus_tag="IWO04_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0213658.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         6396 a         2952 c         3534 g         5556 t
ORIGIN      
        1 tataatgaga acttttacta acataattta tgataaactt actatataat catattaatt
       61 aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat
      121 tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat
      181 tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat
      241 agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg
      301 cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag
      361 atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca
      421 aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga
      481 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
      541 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
      601 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
      661 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
      721 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
      781 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
      841 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
      901 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
      961 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
     1021 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
     1081 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
     1141 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
     1201 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
     1261 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
     1321 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
     1381 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
     1441 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     1501 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     1561 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     1621 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     1681 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat
     1741 tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa
     1801 gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca
     1861 ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg
     1921 ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt
     1981 gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa
     2041 atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa
     2101 actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat
     2161 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
     2221 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
     2281 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
     2341 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
     2401 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
     2461 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
     2521 atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa
     2581 tgaatctctc aagagtatca aaaggattta caattattga tgattatact caaagagctt
     2641 acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa
     2701 ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg
     2761 aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag
     2821 tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa
     2881 gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta
     2941 aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa
     3001 tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc
     3061 acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact
     3121 tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg
     3181 accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa
     3241 aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg
     3301 ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt
     3361 gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca
     3421 agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg
     3481 caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa gattatttta
     3541 ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca
     3601 acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg
     3661 agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc
     3721 ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc
     3781 gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta
     3841 tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg
     3901 ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata
     3961 atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca
     4021 ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg
     4081 ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt
     4141 gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt
     4201 taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat
     4261 atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct
     4321 ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt
     4381 tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa
     4441 gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc
     4501 gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat
     4561 atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga
     4621 ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta
     4681 tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat
     4741 caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat
     4801 ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta
     4861 cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac
     4921 agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc
     4981 ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta
     5041 ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt
     5101 tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa
     5161 ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa
     5221 agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag
     5281 ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg
     5341 gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt
     5401 tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc
     5461 gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc
     5521 aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct
     5581 atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt
     5641 atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca
     5701 tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg
     5761 tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg
     5821 ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc
     5881 tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata
     5941 catttttcta tcaacttcat acgccatact tttggagcag tattctcact gctctttttg
     6001 tatgcggact tatcagctgg ctttattatc actatcgtat agattctata aaacagttag
     6061 agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa
     6121 ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc
     6181 attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca
     6241 ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt
     6301 attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg
     6361 atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat
     6421 tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca
     6481 ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg catacaaatg
     6541 caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga
     6601 ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata
     6661 tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg
     6721 cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata
     6781 tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac
     6841 gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg
     6901 atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct
     6961 ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa
     7021 gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa
     7081 aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg
     7141 taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac
     7201 gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa
     7261 ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc
     7321 aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt
     7381 tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg
     7441 gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct
     7501 actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca
     7561 ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt
     7621 atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat
     7681 tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt
     7741 aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg
     7801 gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc
     7861 aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc agatttggca
     7921 aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt
     7981 ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca
     8041 ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat
     8101 gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac
     8161 cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt
     8221 gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat
     8281 tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta
     8341 accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc
     8401 gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc
     8461 accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat
     8521 ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt
     8581 ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca
     8641 ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga
     8701 gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc
     8761 aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga
     8821 ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc
     8881 attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc
     8941 aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc
     9001 tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt
     9061 tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca
     9121 aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat
     9181 tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag
     9241 actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat
     9301 cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt
     9361 acccagacag caagctccat atatgataaa caaggtgata atgtattagt ggatactatt
     9421 ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat
     9481 gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt
     9541 gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat
     9601 gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta
     9661 attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca
     9721 tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct
     9781 aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt
     9841 acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt
     9901 atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat
     9961 ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag
    10021 gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat
    10081 tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt
    10141 gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc
    10201 atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt
    10261 gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt
    10321 gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa
    10381 atagagggta attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa
    10441 caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt
    10501 atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc
    10561 cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga
    10621 ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc
    10681 agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag
    10741 agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca
    10801 aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga
    10861 agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt
    10921 ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa
    10981 tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa
    11041 tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc
    11101 aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg
    11161 attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac
    11221 accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc
    11281 ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt
    11341 ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt agggattccc
    11401 attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt
    11461 ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata
    11521 cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat
    11581 ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt
    11641 cgcttattat gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca
    11701 agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct
    11761 gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac
    11821 tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc
    11881 tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa
    11941 tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt
    12001 ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat
    12061 cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat
    12121 tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg
    12181 tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt
    12241 ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta
    12301 tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat
    12361 tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac
    12421 tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac
    12481 cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa
    12541 cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt
    12601 agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa
    12661 tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat
    12721 caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag
    12781 caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg
    12841 tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga
    12901 tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt
    12961 aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa
    13021 caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa
    13081 agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt
    13141 taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact
    13201 cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag
    13261 ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa
    13321 acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa
    13381 tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag
    13441 tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc
    13501 agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt
    13561 aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg
    13621 ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg
    13681 aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt
    13741 cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa
    13801 atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt
    13861 ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt
    13921 agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt
    13981 gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa
    14041 caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt
    14101 ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat
    14161 acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac
    14221 aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat
    14281 ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca
    14341 ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt
    14401 ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt
    14461 tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga
    14521 ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt
    14581 attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt
    14641 tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg
    14701 gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta
    14761 aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa
    14821 cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat
    14881 gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt
    14941 tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt
    15001 ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt
    15061 ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc
    15121 attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt
    15181 gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt
    15241 atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc
    15301 ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct
    15361 cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt
    15421 ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa
    15481 gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat
    15541 gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg
    15601 gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca
    15661 ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca
    15721 gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag
    15781 caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa
    15841 aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag
    15901 cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga
    15961 ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta
    16021 aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta
    16081 gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg
    16141 atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt
    16201 caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg
    16261 aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag
    16321 atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt
    16381 ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag
    16441 tggatataga ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga
    16501 ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg
    16561 gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat
    16621 ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc
    16681 caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag
    16741 ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa
    16801 tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg
    16861 acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt
    16921 caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct
    16981 tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc
    17041 atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata
    17101 aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa
    17161 agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt
    17221 ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat
    17281 actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt
    17341 tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat
    17401 aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa
    17461 atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt
    17521 tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa
    17581 gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca
    17641 gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac
    17701 caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact
    17761 agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac
    17821 tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg
    17881 aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta
    17941 gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat
    18001 aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg
    18061 tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
    18121 taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
    18181 aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta gttttttgta
    18241 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata tctgtatgct
    18301 ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
    18361 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
    18421 tttagctcat gttgatgc
//
LOCUS       DACECB010000058        17895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECB010000058 DACECB010000000
VERSION     DACECB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.058"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..326
                     /gene="sgrA"
                     /locus_tag="IWO04_002076"
     CDS             <1..326
                     /gene="sgrA"
                     /locus_tag="IWO04_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0213659.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            624..980
                     /locus_tag="IWO04_002077"
     CDS             624..980
                     /locus_tag="IWO04_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213660.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            983..2140
                     /locus_tag="IWO04_002078"
     CDS             983..2140
                     /locus_tag="IWO04_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213661.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2166..2606)
                     /locus_tag="IWO04_002079"
     CDS             complement(2166..2606)
                     /locus_tag="IWO04_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0213662.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2766..3272
                     /locus_tag="IWO04_002080"
     CDS             2766..3272
                     /locus_tag="IWO04_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0213663.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3440..5352
                     /locus_tag="IWO04_002081"
                     /pseudo
     CDS             3440..5352
                     /locus_tag="IWO04_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            5357..6358
                     /locus_tag="IWO04_002082"
     CDS             5357..6358
                     /locus_tag="IWO04_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0213664.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IWO04_002083"
     CDS             complement(6560..6766)
                     /locus_tag="IWO04_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0213665.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="IWO04_002084"
     CDS             complement(7412..8260)
                     /locus_tag="IWO04_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0213666.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="IWO04_002085"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="IWO04_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0213667.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="IWO04_002086"
     CDS             9328..10329
                     /locus_tag="IWO04_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0213668.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="IWO04_002087"
     CDS             10326..10610
                     /locus_tag="IWO04_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0213669.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="IWO04_002088"
     CDS             10643..11440
                     /locus_tag="IWO04_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0213670.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="IWO04_002089"
     CDS             11454..12287
                     /locus_tag="IWO04_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213671.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="IWO04_002090"
     CDS             12305..13162
                     /locus_tag="IWO04_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0213672.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="IWO04_002091"
     CDS             13184..13693
                     /locus_tag="IWO04_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213673.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="IWO04_002092"
     CDS             13730..14131
                     /locus_tag="IWO04_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213674.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="IWO04_002093"
     CDS             14243..14596
                     /locus_tag="IWO04_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213675.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="IWO04_002094"
     CDS             14630..15073
                     /locus_tag="IWO04_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0213676.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO04_002095"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO04_002095"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0213677.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="IWO04_002096"
     CDS             16168..16881
                     /locus_tag="IWO04_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0213678.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            17633..17833
                     /locus_tag="IWO04_002097"
     CDS             17633..17833
                     /locus_tag="IWO04_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0213679.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6247 a         2772 c         3734 g         5142 t
ORIGIN      
        1 tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata
       61 atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca
      121 acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa
      181 aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga
      241 acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc
      301 gttctctctt ccatcgagtg aaataaagaa tgaaagcaac agatctaaaa ttgaataaaa
      361 cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag
      421 ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac
      481 aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga
      541 aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata
      601 atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag
      661 tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata
      721 tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat
      781 cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa
      841 aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa
      901 cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc
      961 ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac
     1021 tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga
     1081 agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca
     1141 ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca
     1201 tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt
     1261 gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt
     1321 gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc
     1381 aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt
     1441 tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga
     1501 aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg
     1561 ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc
     1621 aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga
     1681 ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc
     1741 tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata
     1801 ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca
     1861 atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga
     1921 acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta
     1981 tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca
     2041 acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa
     2101 aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc
     2161 caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc
     2221 aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct
     2281 tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa
     2341 gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga
     2401 acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc
     2461 aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg
     2521 ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca
     2581 gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa
     2641 taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca
     2701 cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca
     2761 caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt
     2821 ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca
     2881 aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag
     2941 tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt
     3001 ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg
     3061 aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga
     3121 ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa
     3181 cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg
     3241 ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa
     3301 tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat
     3361 ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct
     3421 cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt
     3481 ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat
     3541 ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga
     3601 aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt
     3661 tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac
     3721 agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg
     3781 gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta
     3841 cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt
     3901 aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat
     3961 caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt
     4021 gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta
     4081 tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga
     4141 tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt
     4201 tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg
     4261 gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac
     4321 ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg
     4381 gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt
     4441 attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc
     4501 aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc
     4561 cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct
     4621 tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac
     4681 gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt
     4741 acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt
     4801 agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca
     4861 tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga
     4921 aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga
     4981 aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc
     5041 gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc
     5101 agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga
     5161 acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca
     5221 aaaaaagaca gatgcaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    17101 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    17161 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    17221 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    17281 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    17341 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    17401 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    17461 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    17521 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    17581 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    17641 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    17701 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    17761 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    17821 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    17881 tgtacgaaaa aattc
//
LOCUS       DACECB010000059        17346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECB010000059 DACECB010000000
VERSION     DACECB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.059"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWO04_002098"
     CDS             232..1500
                     /locus_tag="IWO04_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0213680.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO04_002099"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO04_002099"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0213681.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IWO04_002100"
     CDS             3076..3351
                     /locus_tag="IWO04_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0213682.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IWO04_002101"
     CDS             3357..4124
                     /locus_tag="IWO04_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0213683.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IWO04_002102"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IWO04_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0213684.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IWO04_002103"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IWO04_002103"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0213685.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IWO04_002104"
     CDS             8268..8570
                     /locus_tag="IWO04_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0213686.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IWO04_002105"
     CDS             8573..9736
                     /locus_tag="IWO04_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0213687.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IWO04_002106"
     CDS             9762..13190
                     /locus_tag="IWO04_002106"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0213688.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IWO04_002107"
     CDS             13211..14335
                     /locus_tag="IWO04_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213689.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IWO04_002108"
     CDS             14325..14762
                     /locus_tag="IWO04_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0213690.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IWO04_002109"
     CDS             14746..15084
                     /locus_tag="IWO04_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0213691.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO04_002110"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO04_002110"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0213692.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IWO04_002111"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IWO04_002111"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0213693.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IWO04_002112"
     CDS             16241..17284
                     /locus_tag="IWO04_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0213694.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5876 a         3010 c         3837 g         4623 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaa
//
LOCUS       DACECB010000060        17163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECB010000060 DACECB010000000
VERSION     DACECB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.060"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO04_002113"
     CDS             complement(543..2048)
                     /locus_tag="IWO04_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0213695.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO04_002114"
     CDS             complement(2045..2731)
                     /locus_tag="IWO04_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213696.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO04_002115"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO04_002115"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0213697.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IWO04_002116"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IWO04_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0213698.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IWO04_002117"
     CDS             complement(4752..5321)
                     /locus_tag="IWO04_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0213699.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IWO04_002118"
     CDS             5507..6409
                     /locus_tag="IWO04_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0213700.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IWO04_002119"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IWO04_002119"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0213701.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IWO04_002120"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IWO04_002120"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0213702.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IWO04_002121"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IWO04_002121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0213703.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IWO04_002122"
     CDS             12977..13162
                     /locus_tag="IWO04_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0213704.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IWO04_002123"
     CDS             complement(13224..14420)
                     /locus_tag="IWO04_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0213705.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IWO04_002124"
     CDS             complement(14590..16026)
                     /locus_tag="IWO04_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0213706.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IWO04_002125"
     CDS             16319..17128
                     /locus_tag="IWO04_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0213707.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5039 a         3591 c         2980 g         5553 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aaaagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gac
//
LOCUS       DACECB010000061        17007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECB010000061 DACECB010000000
VERSION     DACECB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.061"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IWO04_002126"
     CDS             complement(<1..126)
                     /locus_tag="IWO04_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0213708.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IWO04_002127"
     CDS             complement(230..541)
                     /locus_tag="IWO04_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0213709.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IWO04_002128"
     CDS             complement(543..740)
                     /locus_tag="IWO04_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0213710.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IWO04_002129"
     CDS             complement(1134..2861)
                     /locus_tag="IWO04_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0213711.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IWO04_002130"
     CDS             complement(2854..4047)
                     /locus_tag="IWO04_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0213712.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="IWO04_002131"
     CDS             complement(4234..4878)
                     /locus_tag="IWO04_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0213713.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="IWO04_002132"
     CDS             complement(4972..5106)
                     /locus_tag="IWO04_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0213714.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWO04_002133"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWO04_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0213715.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="IWO04_002134"
     CDS             complement(7237..7710)
                     /locus_tag="IWO04_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0213716.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="IWO04_002135"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="IWO04_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0213717.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="IWO04_002136"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="IWO04_002136"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0213718.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="IWO04_002137"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="IWO04_002137"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0213719.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="IWO04_002138"
     CDS             complement(11757..12896)
                     /locus_tag="IWO04_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0213720.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="IWO04_002139"
     CDS             complement(13205..13918)
                     /locus_tag="IWO04_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0213721.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWO04_002140"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWO04_002140"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0213722.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="IWO04_002141"
     CDS             15107..16639
                     /locus_tag="IWO04_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0213723.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="IWO04_002142"
     CDS             complement(16830..>17007)
                     /locus_tag="IWO04_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0213724.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DACECB010000062        16190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECB010000062 DACECB010000000
VERSION     DACECB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.062"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..447
                     /gene="scm"
                     /locus_tag="IWO04_002143"
     CDS             <1..447
                     /gene="scm"
                     /locus_tag="IWO04_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0213725.1"
                     /translation="TDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSA
                     SSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTS
                     NDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(602..1588)
                     /locus_tag="IWO04_002144"
     CDS             complement(602..1588)
                     /locus_tag="IWO04_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0213726.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1611..2177)
                     /locus_tag="IWO04_002145"
     CDS             complement(1611..2177)
                     /locus_tag="IWO04_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0213727.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2191..2607)
                     /locus_tag="IWO04_002146"
     CDS             complement(2191..2607)
                     /locus_tag="IWO04_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0213728.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3064..4998)
                     /locus_tag="IWO04_002147"
     CDS             complement(3064..4998)
                     /locus_tag="IWO04_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0213729.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5300..6235
                     /locus_tag="IWO04_002148"
     CDS             5300..6235
                     /locus_tag="IWO04_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0213730.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6325..7428
                     /locus_tag="IWO04_002149"
     CDS             6325..7428
                     /locus_tag="IWO04_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0213731.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7441..8394
                     /gene="menA"
                     /locus_tag="IWO04_002150"
     CDS             7441..8394
                     /gene="menA"
                     /locus_tag="IWO04_002150"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0213732.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9208..10554)
                     /gene="gor"
                     /locus_tag="IWO04_002151"
     CDS             complement(9208..10554)
                     /gene="gor"
                     /locus_tag="IWO04_002151"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0213733.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10688..11929)
                     /locus_tag="IWO04_002152"
     CDS             complement(10688..11929)
                     /locus_tag="IWO04_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0213734.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11947..12870)
                     /locus_tag="IWO04_002153"
     CDS             complement(11947..12870)
                     /locus_tag="IWO04_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0213735.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13091..15583)
                     /locus_tag="IWO04_002154"
     CDS             complement(13091..15583)
                     /locus_tag="IWO04_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0213736.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15590..16057)
                     /locus_tag="IWO04_002155"
     CDS             complement(15590..16057)
                     /locus_tag="IWO04_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0213737.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4757 a         3383 c         2880 g         5170 t
ORIGIN      
        1 acggatacat ctaccacaac aagtgatagc acggacacgt cagcttcaag tgacagtaca
       61 gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag tgatagcacg
      121 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
      181 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
      241 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
      301 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
      361 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
      421 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
      481 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
      541 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
      601 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
      661 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
      721 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
      781 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
      841 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
      901 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
      961 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
     1021 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
     1081 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
     1141 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
     1201 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
     1261 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
     1321 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
     1381 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
     1441 acagctttcc gccggcatgg atcgtttctt tgatggcttg ttcgacaggt ttgtttttca
     1501 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
     1561 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
     1621 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
     1681 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
     1741 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
     1801 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
     1861 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
     1921 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
     1981 agtcgtagcc aaagtagtaa gaagctgtct tgttttttca tggtaaaaat ggcaacgatc
     2041 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
     2101 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
     2161 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
     2221 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
     2281 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
     2341 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
     2401 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
     2461 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
     2521 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
     2581 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
     2641 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
     2701 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
     2761 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
     2821 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
     2881 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
     2941 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
     3001 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
     3061 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
     3121 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
     3181 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
     3241 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
     3301 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
     3361 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
     3421 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
     3481 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
     3541 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
     3601 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
     3661 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
     3721 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
     3781 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
     3841 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
     3901 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
     3961 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
     4021 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
     4081 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
     4141 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
     4201 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
     4261 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
     4321 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
     4381 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
     4441 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
     4501 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
     4561 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
     4621 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
     4681 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
     4741 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
     4801 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
     4861 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
     4921 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
     4981 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
     5041 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
     5101 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
     5161 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
     5221 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
     5281 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
     5341 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
     5401 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
     5461 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
     5521 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
     5581 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
     5641 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
     5701 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
     5761 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
     5821 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
     5881 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
     5941 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
     6001 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
     6061 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
     6121 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
     6181 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
     6241 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
     6301 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
     6361 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
     6421 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
     6481 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
     6541 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
     6601 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
     6661 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
     6721 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
     6781 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
     6841 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
     6901 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
     6961 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
     7021 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
     7081 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
     7141 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
     7201 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
     7261 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
     7321 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
     7381 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
     7441 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
     7501 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
     7561 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
     7621 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
     7681 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
     7741 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
     7801 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
     7861 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
     7921 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
     7981 gatttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
     8041 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
     8101 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
     8161 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
     8221 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
     8281 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
     8341 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
     8401 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
     8461 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     8521 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     8581 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
     8641 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
     8701 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
     8761 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
     8821 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
     8881 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
     8941 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
     9001 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
     9061 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
     9121 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
     9181 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
     9241 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
     9301 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
     9361 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
     9421 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
     9481 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
     9541 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
     9601 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
     9661 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
     9721 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
     9781 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
     9841 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
     9901 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
     9961 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
    10021 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
    10081 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
    10141 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
    10201 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
    10261 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
    10321 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
    10381 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
    10441 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
    10501 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
    10561 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
    10621 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
    10681 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
    10741 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
    10801 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
    10861 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
    10921 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
    10981 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
    11041 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
    11101 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
    11161 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
    11221 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
    11281 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
    11341 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
    11401 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
    11461 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
    11521 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
    11581 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
    11641 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
    11701 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
    11761 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
    11821 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
    11881 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
    11941 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
    12001 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
    12061 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
    12121 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
    12181 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
    12241 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
    12301 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
    12361 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
    12421 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
    12481 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
    12541 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
    12601 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
    12661 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
    12721 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
    12781 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
    12841 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
    12901 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
    12961 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
    13021 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
    13081 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
    13141 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
    13201 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
    13261 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
    13321 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
    13381 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
    13441 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
    13501 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
    13561 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
    13621 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
    13681 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
    13741 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
    13801 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
    13861 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
    13921 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
    13981 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
    14041 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
    14101 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
    14161 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
    14221 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
    14281 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
    14341 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
    14401 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
    14461 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
    14521 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
    14581 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
    14641 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
    14701 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
    14761 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
    14821 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
    14881 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
    14941 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
    15001 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
    15061 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
    15121 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
    15181 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
    15241 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
    15301 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
    15361 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
    15421 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
    15481 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
    15541 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
    15601 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
    15661 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
    15721 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
    15781 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
    15841 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
    15901 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
    15961 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
    16021 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
    16081 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
    16141 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACECB010000063        15932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECB010000063 DACECB010000000
VERSION     DACECB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.063"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            134..3661
                     /locus_tag="IWO04_002156"
     CDS             134..3661
                     /locus_tag="IWO04_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0213738.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3658..7380
                     /gene="addA"
                     /locus_tag="IWO04_002157"
     CDS             3658..7380
                     /gene="addA"
                     /locus_tag="IWO04_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0213739.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7449..7790
                     /locus_tag="IWO04_002158"
     CDS             7449..7790
                     /locus_tag="IWO04_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213740.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8099..9142
                     /gene="pheS"
                     /locus_tag="IWO04_002159"
     CDS             8099..9142
                     /gene="pheS"
                     /locus_tag="IWO04_002159"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0213741.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9147..11567
                     /locus_tag="IWO04_002160"
     CDS             9147..11567
                     /locus_tag="IWO04_002160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0213742.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="IWO04_002161"
     CDS             complement(11737..12747)
                     /gene="trpS"
                     /locus_tag="IWO04_002161"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0213743.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13001..13738
                     /locus_tag="IWO04_002162"
     CDS             13001..13738
                     /locus_tag="IWO04_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213744.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13752..14567
                     /locus_tag="IWO04_002163"
     CDS             13752..14567
                     /locus_tag="IWO04_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0213745.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14580..15227
                     /locus_tag="IWO04_002164"
     CDS             14580..15227
                     /locus_tag="IWO04_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0213746.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15243..15899
                     /locus_tag="IWO04_002165"
     CDS             15243..15899
                     /locus_tag="IWO04_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0213747.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5341 a         2745 c         3376 g         4470 t
ORIGIN      
        1 ttttttgcgt atacttaaaa aagtagaaat atcagaacct taaaaggata taatttgaag
       61 aatatagaac aaatgttcag tattttttga tataataaag gagcaccgta tcatgtgttg
      121 aaggaggaaa agcatgtctt tacaatttat tcttggaaac gggaaaaaag atcaccgtca
      181 agtcttgatc gacgaagcat ccgcttggct cgaccaggat gcaagtaacc aagtattttt
      241 ccttgttccc aactacagca aattcgaaca ggaacaagaa attttagcgg agatgcgaag
      301 aagaagcgga aaaaaaactt tcagcacaac aaacgttcaa gtatttagtt tttatcgtct
      361 ggcttggtat ttgctgcaac agacaactct tcttacagga aatgaattat cagaatcagg
      421 atctgcaatg atcttgcgac agattttaga aaaaaatgag gaagaattaa caatctttcg
      481 cggagaaatc aataaaagag ggtttattcg tcaattacaa gaactctaca gtgaattgcg
      541 tacaggaaat attcaaccag aagacctgat gcttttattt ggtgattcac ctaatgcaga
      601 agaccagcaa cttaaaatga aagacttgaa gctgattttt tcagcttatg acagcgaatt
      661 gactcaacgt gcactaacaa atgaagatgc actatctata ctagcaaatt atatgcagac
      721 acaagatttc aacaacgtca aatttattgt cagtggtttt tctcgaatca atggtcaaga
      781 gtatcaatta ctgcaggcaa tgatggaaaa ggggcaattg gttgtcgatt tattgctgga
      841 tcgtccttat acagcggatc ttccagaagt acttacgctc tttcatgaga ctggaaagct
      901 ttatttccgt ctttttcaaa gtgcaagaga acggaaagtc cctatattag tggaccgatt
      961 tgcaccagag cagactcgtc cgacagagct tcaactgctg caaacaatgt gggaagagac
     1021 atctagccaa aagaaagcgg cagtacacaa aataggggga caagagcatc tccatttgtg
     1081 ggaagctgaa aatccaactg aagaagtccg acaattagct gttgaaatca gacgactagt
     1141 cagtgaagaa aactaccgtt atcgtgatat ccaagtgctg acaaaaaata tgtctttata
     1201 tggaaatatc cttcggccga tctttcaaga aatggcaatt cctttttata tcgatgaaga
     1261 acaaatgatg gaaaatcatc cgctgatcga atggatcaac agtctgttcg ctcttgaccg
     1321 ctattcttat cggctaagtg atattatgcg ttttttcaaa actgaattat ttctgtcgac
     1381 ggaagcagaa accgaacttg atgaatggaa acaagagcga gatgaattca ggaaaaaact
     1441 ggacatcact gaaaatgtag cacttgctta caattatcaa ggaacttatt ggactcgtga
     1501 aaaagactgg cagtttgtgg cgtatgattt tgaagcagaa cagattgaag acgtacagta
     1561 tttggaagaa cagtcaaata cgattagacg attatttcag aaagctgtac cagtattttt
     1621 gaaaaaaata aagaaaagca aaacaggaca agaggccgca aatgtttttt atcaattttt
     1681 gatccaaagt ggcgtacagc aacaattatt gttttggcgt aatcaggaag tagacagagg
     1741 aaacttagaa gcggcaagaa accatgagca gacatgggga gcgctgattg atctgttgga
     1801 tgaatatgta gtaatttatg gtgaatcggc ttttgactgg tcactgttcc aagaaatcat
     1861 tacaagtggt ctagaaaatc taagttatgg taaaatccct accgcaatcg accaagtgcg
     1921 aatcaatcgt ttggaattag ttcgaccaaa tcaagctgcc gtcacttttg cattagggtt
     1981 gaatgatcaa gtgtttccag accgaaaaga gacaaaagga ttgttgtcca gtgaagaacg
     2041 agaagtatta aatcaggcct tgccagatgg acagttttta ttcgacccat caaaggaaag
     2101 tatctctttt gagcctttcc aagcctatct tgtttttctt tccggaacag atcgattata
     2161 tcttagttat gcacaaagct atgacacaga tggttcgctg aagatgtctc cttatttgcg
     2221 tagaatcaca gaatatttat ctgttcccgt cgagagaaaa gagcatttga cgatcgaaag
     2281 cgaccctaat tgttatgtag ggacttacag aagcggaatc aatacagtca ataggattta
     2341 tcgattagct ttagatgaaa aaaaacctgt accaaagcac tggcaaaagc tcaaagaatt
     2401 agtactgaat tcgtcatggc gaaattttgc tttacagatt tttgaaagtc aggaacattt
     2461 aaatgttcct gtttcgatgc cagaaaaaat ggcggaagag ctttatggaa aagagattta
     2521 tacttcaatt tcacgtatgg agaattttta taattgtgaa tacaaatact tcatgcaatt
     2581 tggtttgaaa ttgaaagaac gaactgttta tggtctaact ccagcagcaa ctggtgattt
     2641 ttatcatgaa tcattggatc gtttcttcaa gctactgttt tcaaaccaat tatccttagt
     2701 gaatatgtcc gatgcgcagc gtagagactt tactgaaaaa gtgctgcagg aagtattcgg
     2761 agaacttcgg tttgagatat tggatagttc tgcaagaatg aactatatcc gctaccagtt
     2821 aggacagacg attcaaaaag tgacatgggc tttgcaaaaa cagagcaaaa atacaggcat
     2881 gcatcctctt cagacagaga ttctatttgg tcagattgct ggagcaaaag gtatcacagg
     2941 tctggaatta ccgttgagta atggtggaaa actccatgtt cgagggaaaa tcgatcgaat
     3001 cgatgtagct tctgaacaag aagatacttg gctaagcgtc gttgattaca aatctagcgg
     3061 acgttcattc gatgtcacag aagcttatta tggcatggct atgcaattat tgacgtatct
     3121 ggacgtagca ttgatggatg cggtacgttt gactggaaag gcggctgtta aaccagcagg
     3181 atcatattat ttacatgttc ataatcctgt attgacggag acagatgatg tagagaagaa
     3241 tacattgaaa aaatttagtt atgatggcgt ttttgtcgat gatcccgctc ttttagaagt
     3301 actagatcat tcactggaag caaaagaaca atcactgatc tatccagtga aaaaaaatgc
     3361 gaaagatgaa taccaaaaag gtagtttcag taaagacaaa tttttcacgg aagaagaact
     3421 gcaactattc atggcgcata atcgggaaaa tatgcggtta gctggagatg cgattacttc
     3481 cggagaaatc aaactaaatc cagcttataa agataaacag cggattgcct gcgaattttg
     3541 tccattcaga agcatctgta cgtttgatgt gatgctgaaa gaaaataatt accaccgtct
     3601 ggagaaaata gataaggaag aagcactgcg tagaatattg aaaaggagtg aagaagagtg
     3661 aatcaaattc ctttaaaacc gcaaaatgaa cgattcacag atgatcagtg gcaagcgatt
     3721 tttgatcaag gagataatct attagtctct gcgtctgccg gatcaggtaa aactacagtg
     3781 cttgttcgaa gagtgattga aaaactaaaa atgggttttg atatcgatga attattgatt
     3841 gtgacattta ctgaagctgc cgcaagagaa atgaaagaaa gaattcaaga ggcattacaa
     3901 gaatccgtca atagcgaaag tgaccctgta cgtagacagc attttactaa gcagttagtt
     3961 ttattgccca cagcgaatat ctcaacctta catgcttttt gcctgacagt tattcgacgt
     4021 tattattact taattgatat tgatccagtc tttcgcatgt taacagatga aacagaaacg
     4081 atcttgatga aggaggatgt gtgggacgag ctgagagaag cactttatgc agaaaatgat
     4141 gaacgatttt tccaattaac aatgaatttt tctaatgatc gttcagatga cggcttgacg
     4201 aatctggtgt tttctcttta tgagtttgcc cgtgccaacc ctgaccccca aaaatggcta
     4261 gaacagttaa gcgataatta tcgtttgcct gaggggctag cgaaatctcg attatatcaa
     4321 aaacaaatcc gtcctttagt tttggcagat atataccaat gtgtacagtt atatgaacag
     4381 atgactcagt tagcacaagg tgaaggatta gaaaaaatgc atgagcaagt agctggagaa
     4441 caacagcaga tcaagaatat ctatgaagcg ttctcgcaag atcggttaga agaagcttat
     4501 gctggtttgg aacaactaac tttttcaact tttaagagca gtcggaaagc agaattgaaa
     4561 gaaatttcca atgaagtcaa gggcatgagg gataaagcaa aaaaactgat tcagcagata
     4621 agtaaaagtt attttcctgt ttccccaagc caaatggaag aattaacgga caaagcacta
     4681 ccgttagtcg aagaaatgac aaaagtcacg caaagcttca tggacggttt cagtatgcgt
     4741 aaacgggaaa aaggtgtgct ggatttcaat gatctggaac atttagcgtt gcagattctc
     4801 acagaaaaaa cgaaagatgt atggctgcca agtgaagctt caaaacatta tcgaaaaaaa
     4861 ttcaaagaag taatggtaga tgaatatcaa gacgtgaacc aattacaaga agccatttta
     4921 tactggctaa gagaacccga tgatacaaaa ggaaatatgt tcatggttgg ggatgtcaaa
     4981 caatccatct attctttccg tttagcagat cctagtttat ttatcgggaa atacgagaat
     5041 ttctcaaaga aagaaggcgg acgtcgaatc gttttggctg agaacttccg ctctagaaaa
     5101 gaagtccttt cttttacgaa tcttattttt gaacaactaa tggatccagc agtgggtcaa
     5161 atcaattatg acgaagcagc caaactgatc caaggatttt cagactttcc agagaatgaa
     5221 cagtttgagc cggaaatcat gatctatgaa aaagaacagg aagaatcaga gattgagatt
     5281 ccgacggatg atatactgga agacaaaaca gaaggcgaac tttttatgac aggactaaaa
     5341 attcgtcaat taattgattc ctcctttatg atttatgata aaaaatcaaa aaaaagtcgt
     5401 ccgatagagt acaaagacat tgttctcttg acacctacaa agaagaacaa cttaacgatt
     5461 ttagagattt tcaaaacatt ggatattcca ttagagatga atgatgctca aaactatttt
     5521 caagcgacag aaattcgcac gatgatctcg ctgctacaat taatcgataa tccctatcaa
     5581 gatattccgc ttgcggctgt attgcgctca ccgatcgtgg gtctgattga accagaatta
     5641 gccagcattc gattagctaa ccgtgcacat acttactatg atgctgtgct tgcttatcaa
     5701 gcatcaaacg aagatgaatt agcagctaag ctggaacatt ttggcaagca actagagcat
     5761 tggcgagaac ttgctcgccg ttcttcgatc actgacttgt tatgggatat ctattatgag
     5821 acgggatatc tagagtatgt ggtcggtctg ccagcagggg cacagagaca agccaatctt
     5881 tatgcattgg ttgatcgtgc caaagcttac gagcaaagtt ctttcagagg attataccag
     5941 ttcgtgcgtt tcattgaaaa aatgcaagaa aaagataaag acttagcaga accagtcatt
     6001 tcaatagaag ataatgctgt tcgcgttatg acgatccatg caagtaaagg cttggaattc
     6061 ccagttgtat ttttgttaga tatgacaaag gagttcaact tacaagactt gcgcaatcgg
     6121 tatgcttttg aagaaaaact tggagcagga attcgctata tggatcctga aacacgagtg
     6181 ttatatgata cgttaccgtt tcaagccatc aaattggcaa agcaaaacaa attgttgtcg
     6241 gaagaaatgc gtaaattgta tgtaggattg actcgggcag aacaaaagct tttcattgtc
     6301 ggttcctata aaaataaaga gcaaatgatc caaacgtggt ctgaagcagc tgatcatgag
     6361 gagttagttt ttgatcctgc gctacgtttg aagggcagaa gcagcttgat gaattggatt
     6421 ggttacggat tgatccgtca tcctgaaatg cagaaatact tagaggaaga aatttcgact
     6481 tctcttcttc aacattcaaa tgcacagttt tctatttcat ggatgaatca gcaatcgatc
     6541 attgaacaaa gacagttgct tgctgaaaaa gaactggtta acttagatca gcagatgaaa
     6601 gaagatgaaa ctttattagc agattcgtta caaaaacgtc tggcatatga atatccttat
     6661 caagcttctt cacaaaccac aagttatcaa tctgtatctg aaatcaaacg attattcgaa
     6721 gatccagatg atacacaaga atcacgtttg actctggaaa gcagtcagaa caaagcagca
     6781 tcccgtcaat ttcgttatac gcaagaacaa ctagcagaac caaaattttt gcaaaaagat
     6841 cggcaagtgt cagcagctgc tgttggaact gctacgcatg ctttgttaca attactgcca
     6901 ttagagatgc caactacaga gagtatccat caaaaattac aggaattggt aaggaaacgg
     6961 ttagtagacg aaaaagtcgc gaaaaaagta gatgtttctt ctattatatg gttctttcaa
     7021 acagaacttg gtcaacagct gattgccaac aaagaaaacg taaaacgaga acaacctttt
     7081 tccatgctgc ttcctgctga cgaagtattt caagactatc caaatcaaga agatgaacta
     7141 ttaatccacg gaatcgtcga tggataccta gaagaaaaag atcatctcaa tatctatgat
     7201 tttaaaacag attttatttt acatccggat gatccagcag aaatagatgc aatcgtccaa
     7261 aaatatcaag gacagctaag actttaccaa caagcaatgt cagaagcttt gaataagcca
     7321 gtagaaaatg ttttcctaat tttgcttcga gtgaaacaaa ttataaacat taacaaataa
     7381 ttataattga gcaaacttga cttatgtttt gtaaggatta tgctatactc tgggaagagg
     7441 tgagtatgat ggagctaaca cagacagaat tcgttttatg ccctaaattt gaaaaaacat
     7501 tctctatttt aggaaaaaaa tggaacggat tgattatcga tgtactactc gaaaacggtc
     7561 cgcaacgctt tgttgagctt gcggcaaaaa tcccgatggt cagtgatcga gtattagtcg
     7621 aacgcttgaa ggaattagaa aaagaaggaa tagtcactcg aaactttgcc tgtagcgaaa
     7681 agagccgttc agaatatctc ttgactgaaa aaggagcaga cttgcaaaaa gcgatgcgtc
     7741 aaatacaagt ttggagcgaa aaatggatca cagaggaaga atgcggttga ttttgtaaaa
     7801 aaaatcttgt aaactatagt ccatacgaat aagtaaagaa cgatgacgaa aaagagtagt
     7861 ttggctaaac cagttcaggg agatctgtcg cagactgaaa gcagatcctg tgtttgaaaa
     7921 atgaagttca ctttccgagt tcgtctgata aaatcagacc ggcaattgtc gttaacgaag
     7981 taagagtgcg gtgtaagtca tttatccgca aattaggatg gtaacgcgaa gacctcgttc
     8041 cttaacagga atgaggtctt tttgttttgt atttggaatt taaaggagga acaagcacat
     8101 gtctctacaa gcacaattag agagcttgaa agaagcaacg ctcgtcaaaa tcacagcggc
     8161 acaagattta aatgaattaa atcaaatacg tgttgaaacg ttaggaaaaa aaggaccgat
     8221 cacagaagta ttacgtggga tgaaagatct aagtccagaa gagcgaccaa aagtcggaag
     8281 tttcgcaaat gagatccgag atcttttgac agaagagatc gagcgaaaaa aacaagtatt
     8341 agaagaagaa gcgttaaatg cagctttggc aaaagaaaca atagacgtga cactacctgg
     8401 tagaaaagta aaacatggta cacgtcatat cgtgacacaa atcatggaag aaattgaaga
     8461 tatttttgtg ggtatgggct atcagatcgt agaaggatat gaagtagaat ctgatcatta
     8521 caatttcgaa cggatgaatc tgccaaaaga tcatccagca agggacatgc aagatacttt
     8581 ctatatttca gacgagatat tgattcggac acatacttca ccagtccaag cacggacaat
     8641 ggaaaaacat gatttctcta agggtgctct acgaatgatc tcgccaggga aagtcttccg
     8701 cagagataca gatgatgcga cccacagtca tcagttccat caaattgaag ggctagttgt
     8761 tgataaaaac atcacaatgg gcgaccttaa aggaacatta gaagtcgtaa tgaaaaaaat
     8821 gtttggggaa gatcgtgaaa ttcgtttgcg tccaagttat ttcccattta cggaaccatc
     8881 agtagaagta gatgtcagct gtttcaaatg tggtggtgcc ggttgtaacg tatgtaaata
     8941 cactggatgg atcgaaattt taggagctgg catggtacac ccgaatgtgt tgaagatgtc
     9001 aggaatcgat ccagaagaat attcaggctt tgctttcggc cttggaccag accgcgtagc
     9061 tatgttgcgg tatggagtga atgatatccg taatttctat caaaatgact tacgtttctt
     9121 aagtcagttc aagggggagt aagaaaatgt tagtttcata taaatggctc aatcgttatg
     9181 tagatttatc gaatgtaaca ccaaaagaat tagcggataa gatgtctgtg acaggaatcg
     9241 aagtagaggg agtaacggtt ccagaagaag gcttgaaaaa aatcgttgtc ggtgaagtca
     9301 aggaatgcgt accacatcca gattctgatc acctttctat ttgtcaagtc gatattggcg
     9361 aagacgaact gtctcaaatc gtttgtggag cgcctaatgt gaaggttgga atcaaagtga
     9421 ttgttgcact tcctaactca cgtatcgctg gtaatgtaaa aatcaaaaaa ggaaaaatgc
     9481 gtggccaagt atcaaatggc atgatttgct cattacaaga aattggctat tcagatagtg
     9541 ttataccgaa agaatacgct gaaggtatct attatatgcc acaagaagct gtaaatggcg
     9601 atccagtttt tccttatttg gatatggatg attcgattat tgaattatct atcacaccga
     9661 atcgtgcgga tgcattgtca atgcgtggcg tagcttatga agttggggca atttatcgtc
     9721 agactcctac attcgatgac gaaaagctgg tagaatcctc acgtcctgct agcgataaaa
     9781 ttcaagtgga agttgaagat aaagaagcag ttcctgctta tcaaatcaga atcattgaag
     9841 gagtaagaat ccagccaagt cctcaatggc tgcaaaatct tttgatgaac gaaggcattc
     9901 gtccaatcaa caatgtagta gatgtgacga actatatttt gcttttattc ggacaaccac
     9961 tacatgcatt tgactacgac aaattgaaat caaaagagat ctttgtccgc cgtgcaaaag
    10021 aaaatgaaac attagtaaca ctagatggag aatctcggga attgacaact gacaatctcg
    10081 tcattactaa cggcaaagaa ccagttgctt tagctggtgt catgggaggg ttagactcag
    10141 agatcacgaa tgaaacaaca acagttgcac tagaatctgc tttatttgat ccaatttcga
    10201 ttcgtcgtac agcaaaacaa tttaatttgc gtagtgaatc ctctgcacgt tttgaaaaag
    10261 gaatcaacca tgcgactgtt ggagaagcca gtgaggttgc tgcagcaatg atcgtttctc
    10321 ttgcgggtgg ggaagttcta caaggcgcgg tcaaaggctc agagatcaaa gcagaagacg
    10381 tatcagtgaa gatttcactt gaacgcatca atcaatattt aggaacatct cttgtcgtta
    10441 aagaagtaaa tgagattttt gaagcacttg gatttagcta tgaagaaaag gagtatgttt
    10501 attccgtagt agtaccacca agacgctggg atatccaaat cgaagcagat attatcgaag
    10561 aagttgctcg tatttatgga tacgaccagt tgccatctac acttccaagt ggagaaacgg
    10621 ttgctggaag cttgtcaaaa gaacaagaaa ctactcgtaa aattcgtaca atctttgaag
    10681 gaagcggatt gagcgaagca atcagttatg cattgacaac agaagaaaaa tcaagacagt
    10741 ttgttataaa tgaaagtaat attacacgtt tggactggcc aatgagcgaa gaacgttctg
    10801 tattacgaat gaatttgatc agcggactat tagatgatat cagttataac actgcccgaa
    10861 aaaatacaga gattgcttta tatgaaattg gtcgggtgtt cttccaagaa aatgatccac
    10921 gtacacatct gccgcaagaa gtaaaacatg cagcaatcgc tatttctggt atttgggaag
    10981 aaaaagattg gcagacgaaa gctcaaaatg tagacttttt caccatcaag ggtcttttag
    11041 aaaacttatt tgaacaacta ggcatcactg aagatgtcca ttatcaaaca gtgacagaaa
    11101 tgaaggaaat gcatcctgga agaactgctg cgatttacct tggcgaaaaa tttattggtt
    11161 ttgtcggtca agtgcatcca acgattacaa aaagttatga tattccagaa acatatgttg
    11221 ctgagtttga tttatctgct atagtggaag ctgctcaaaa agggatcgtt ttcgaagcag
    11281 tgacaaaatt cccagcagtc agccgggaca tcgctttgct tgtaaaagaa actgtgacaa
    11341 atcaggaatt aacagaagtt atccgttctg ctgccggaag atttttaaca gatatccaat
    11401 tatttgatgt ctatcaagga gaaaacattg aaaaaggaca taaatccatg gcgtatagcc
    11461 tgacattcgt caacccagaa gcaacattga cagatgaaga aatcaataaa gcaatggaaa
    11521 aagtcgaaaa agcgttggta gaaaacctag aggcaagtat tcgctaagaa ggaaaatata
    11581 gtaagtaacg atcaagtagc ttttgaagac catacatttg aaaaaaagat gtgacacaac
    11641 ttcgtcacat ctcttttttt gattttaaaa aaggaatagc acagagacga gataaaaaaa
    11701 caggccttta cagcctgctt ttttagagta aatgaattaa tggaagtaat tgattcccat
    11761 tgctgatttt acttcagaaa gtgtttgtgc agctacttta gcagcttctt cactaccttt
    11821 acgtaagatt tccataacag ctgaaggatc tttttccaat tcttctcggc gcgcacggat
    11881 cggtgcaaat tcagcttcca atacatcaat gaggtatcgt ttgatcttta catcaccaag
    11941 tccgccgcgg cggtaatgag ctttcatttc ttcaattgct tctttatctg ttccgaatac
    12001 atccaagtag gtgaagacca tattcccttc tacttgtcca ggatcttgta catggatgtg
    12061 gtttggatca gtatacatac tcatgacttt tttctgtaag acatcggcag gatcggagat
    12121 ataaataccg tttcctaacg atttactcat tttgccattt ccatcgattc caggtaatct
    12181 acccattcct tttggtggga atacgccttt tggttccacc aagacttcac catatgtatg
    12241 attgaagctt tgtacgattt cctgtgtctg ctctagcatt ggtttttgat cctcaccaac
    12301 aggtacaaga ttcgctttga atgcagtgat atctgctgct tgagaaacag gatagataaa
    12361 gaatcctgta ggcacgcttt caccgaattt tttttgttcg atttctgttt tgacagtcgg
    12421 attgcgccga acgcgtccta cgctgactaa gttcaaataa tacatggtta attcagctaa
    12481 ttccggaatt tgtgattgga taaataaagt agattttgca ggatctaatc ccacggctaa
    12541 atagtctaaa gcaacttgca agacatttga tgaaaccttc tcaggatttt tagcattatc
    12601 tgtcagtgct tgcatgtcag caatcatgac aaacagttgg ttgtcttcat ctgcctgcat
    12661 agccactcgg gtttttagag aaccgacgta atgccctaaa tgcagcttcc cagttggtcg
    12721 atctcccgtt aaaataatat ttttcaaaaa agacacctct ttacgatttg taagtctgag
    12781 cacttaatat cttaccgttt ttcctgaaaa gaaacaacag ttcctaagga aaaaacaagg
    12841 aaagaggata aaataactaa tagattagaa atggaagaaa tatttatggg agaataaacg
    12901 ttctaagaaa ctttacaaat tactaaacta tcagatataa tgtattttat tatacatctt
    12961 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
    13021 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
    13081 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
    13141 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
    13201 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
    13261 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
    13321 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
    13381 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
    13441 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
    13501 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
    13561 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
    13621 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
    13681 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
    13741 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
    13801 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
    13861 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
    13921 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
    13981 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
    14041 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
    14101 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
    14161 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
    14221 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
    14281 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
    14341 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
    14401 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
    14461 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
    14521 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
    14581 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
    14641 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
    14701 aattatcaaa gaataaagta gtgagcacct tagcaaaggc atatgtagaa ttttttagaa
    14761 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
    14821 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
    14881 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
    14941 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
    15001 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
    15061 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
    15121 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
    15181 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
    15241 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
    15301 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
    15361 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
    15421 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
    15481 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
    15541 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
    15601 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
    15661 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
    15721 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
    15781 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
    15841 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
    15901 agatgattct tttagcggtt gtggcaaact aa
//
LOCUS       DACECB010000064        15697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECB010000064 DACECB010000000
VERSION     DACECB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.064"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..212)
                     /locus_tag="IWO04_002166"
     CDS             complement(12..212)
                     /locus_tag="IWO04_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0213748.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="IWO04_002167"
     CDS             complement(517..1749)
                     /locus_tag="IWO04_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0213749.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2006..2575
                     /locus_tag="IWO04_002168"
     CDS             2006..2575
                     /locus_tag="IWO04_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0213750.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2753..3193)
                     /locus_tag="IWO04_002169"
     CDS             complement(2753..3193)
                     /locus_tag="IWO04_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0213751.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3351..4115
                     /gene="map"
                     /locus_tag="IWO04_002170"
     CDS             3351..4115
                     /gene="map"
                     /locus_tag="IWO04_002170"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0213752.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4147..5070
                     /locus_tag="IWO04_002171"
     CDS             4147..5070
                     /locus_tag="IWO04_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0213753.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5146..6285
                     /locus_tag="IWO04_002172"
     CDS             5146..6285
                     /locus_tag="IWO04_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0213754.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6278..7078
                     /locus_tag="IWO04_002173"
     CDS             6278..7078
                     /locus_tag="IWO04_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0213755.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7078..7905
                     /locus_tag="IWO04_002174"
     CDS             7078..7905
                     /locus_tag="IWO04_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0213756.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7883..8617
                     /locus_tag="IWO04_002175"
     CDS             7883..8617
                     /locus_tag="IWO04_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0213757.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8717..9583
                     /gene="rfbA"
                     /locus_tag="IWO04_002176"
     CDS             8717..9583
                     /gene="rfbA"
                     /locus_tag="IWO04_002176"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0213758.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9597..10169
                     /gene="rfbC"
                     /locus_tag="IWO04_002177"
     CDS             9597..10169
                     /gene="rfbC"
                     /locus_tag="IWO04_002177"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0213759.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10191..11219
                     /gene="rfbB"
                     /locus_tag="IWO04_002178"
     CDS             10191..11219
                     /gene="rfbB"
                     /locus_tag="IWO04_002178"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0213760.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11317..12168
                     /gene="rfbD"
                     /locus_tag="IWO04_002179"
     CDS             11317..12168
                     /gene="rfbD"
                     /locus_tag="IWO04_002179"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0213761.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12202..14235
                     /locus_tag="IWO04_002180"
     CDS             12202..14235
                     /locus_tag="IWO04_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213762.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14279..15559
                     /locus_tag="IWO04_002181"
     CDS             14279..15559
                     /locus_tag="IWO04_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0213763.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4797 a         2705 c         3267 g         4928 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccct caataaacag tcgaagagaa aataggcaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaac agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccgacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaatg acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgcagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccagccgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacactg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggccttt aaataaaaaa aagaggttat ttttttaagg
     1861 ttaagtttca tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact
     1921 aattttgcat atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
     1981 aaaaaattaa ggagattaaa aaaatatggc aagaaaaaag acgattacta gagaacagat
     2041 tttggcagct gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa
     2101 catcgcgaac aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga
     2161 tgacttgaga caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac
     2221 tgttgagcat acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag
     2281 aagagagaag aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat
     2341 gcagaatttc tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa
     2401 tctttcagaa gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat
     2461 cgcagctttg atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat
     2521 acaagacagt atagatgcta tcttgaaatt caaagaacca atagaaatcg attgaatata
     2581 ctcaaaagtt ccacaaatca gtagataaca agagatgtgt caaaataagg aaaagttcgt
     2641 ttttcttaca gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata
     2701 aaagagccgt acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt
     2761 ttgcgactag ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca
     2821 gatccacttt aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga
     2881 aatcatccac cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt
     2941 aaatttttcc cgatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag
     3001 gcagatcgcc atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt
     3061 catctgcatc tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg
     3121 cttctgcaac aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt
     3181 ttgctaaagc catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca
     3241 aaacatcatt gaaaagaaaa taaaacctaa agacacctgg ggaatgtcat ttggctaaat
     3301 gtatgccgct atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt
     3361 taaaatcagc acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac
     3421 ataaaaatct gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg
     3481 tcagaaacta tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg
     3541 aatatgcgac ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc
     3601 ctttgaaaaa cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt
     3661 ctgattcttg ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg
     3721 aagtaacgaa gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg
     3781 gtgacatcgg tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg
     3841 attttattgg tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg
     3901 gagaagctgg aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg
     3961 ttaataccgg aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag
     4021 acggcggact ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa
     4081 ttttgacatc acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag
     4141 aggtacatga aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag
     4201 acgactcaaa agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat
     4261 tatttactct tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc
     4321 cacatcgatc aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac
     4381 cagtttatcg gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc
     4441 gtttctgcat tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg
     4501 atgaataaag cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca
     4561 ctaattgtca ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg
     4621 ggacagctga ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat
     4681 cagttccaaa ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg
     4741 atttatttaa ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc
     4801 ttcgctactg tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt
     4861 gcaacgagga tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt
     4921 aattttgctg ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac
     4981 gtgacgggtc atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg
     5041 aaaactaaat tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc
     5101 agtaatttat aataattatg actcttaatt aaagtaggtg cccatatgct tgtttctttt
     5161 agtatggtta taagattttt tctgacaatt ttattatcac tgatattaac acctgtattc
     5221 aaaatcattt cagtacaaac aggtatggtc gacaaaccaa atgaaagaag aataaataaa
     5281 gttccaatgc cttcagcggg cggactgcct atctttattt catttgttat atcgacttta
     5341 ttcttattcc gtaaaattat ccctcaatcg tatattgtac cgatcttgat tgcttcttta
     5401 gtaatcatag tcacaggagt gctcgatgat aaatatgagc tgacacctaa acaaaaaagt
     5461 atagggatct tgattgcttc tcttattatt tattttgtag cagatatacg tattgattcc
     5521 ttcacgcttc cttttattgg atatatccaa ctaggctggc ttagttttcc cgtaacgatt
     5581 ttttggatct tcggtatcac gaatgcggtc aatctgattg acggtttgga tgggctggcg
     5641 gcagggattt cattgatcgg tttaattacg ataggaatca ttggatattt tttcctccat
     5701 gcttctacag tgtatattcc agttgttatt ttctgtcttg tagcaagtat tatcggtttt
     5761 tttccttaca atttttatcc ggcaaagatt tatttaggcg atacaggagc tctattccta
     5821 gggtttatga tggccgtcct ttcactacaa gggctgaaga acgtgacttt tgtttcatcg
     5881 atttctttgc tgatcgtcat gggagtgccg gtaacagaca cgttttttgc tatcattagg
     5941 agaaaagcaa accgtgtttc tttttctaca gcagataaaa aacatcttca tcaccgacta
     6001 atcagtttag gctttactca taaaggtgct gtcctgacga tttatgcgat ggctttgatg
     6061 ttttctttca cagctatggt catgaattat actggaagaa tcggaacgat catattgatt
     6121 attgccatgt tgtttgctgc aattttatta tttgaattga ttggtctgat caatgagaaa
     6181 catcagttta ttttaaaaac attgcgattt ttagggaata aagagtaccg tacgcaagta
     6241 aaacagaaat atttgaagaa atggaaccgt catatagatg aataacaaat taaaccagcc
     6301 gatccttatt agtattgtga cggcaaacag tgataaaatt tttcggacac tagataattt
     6361 tatttcaact gtaaaagatg aaacagcagt aaaaatccga atttttgaca acaattcact
     6421 tcccgagtac aaaaaaagac taacagagta taggaaatat ccctacatcg atatctattt
     6481 tcatgatgaa aattccggat ttggttatgg acataatcac aatctgatag ctagcgattc
     6541 tcgctatggc attatttgta acccagatat tttagtggat gaagagacag ttacagcatt
     6601 ggtcttgtta ttgaaagaac atccggaatg tgcgatgtta gcacctaaga ttcttaatga
     6661 agatggaaca actcagcacc tcatccgtga gcagctggct gtttttgatt acgttttgcg
     6721 ctttgtgcct ttcaaatggg tcaaaaaaaa gtttgacaaa cgactggctg tgtttgaatg
     6781 ccgaggattg cctactgatg ctaatagtta tgtacggatg atctcaggta gttttatggt
     6841 agctgatatt gataaattta aagagataaa ggggtttgat gagcgtttct tcatgtactt
     6901 tgaagacaac gacttgtgtt tgagttttga aaaagcaggt tacaagttat tgtacacacc
     6961 cttttatgca gttatccatt tttacgggaa gggtgcgcat cgaaatgcaa aactgtttcg
     7021 cgtgtttttg acttctatga tgaaattttt caataagtgg gggtggcgtt ttttctgatg
     7081 aaaccagaaa tattagtagt cgttgtgctt tatcagcaac tattcagtca atctccagct
     7141 tatggaccat taagcaaagc acttagcgaa aagcaaattc agcttattat ttacgataat
     7201 agtcctcaaa ggcagcaaaa tgagctgttt gaaaaagaaa atatcgttta tcatcatgat
     7261 ccgcaaaatc ctggtttagc ggtggcttat aattttgcgt tgtctcaagc agatgaagga
     7321 acacgttgtc tcgtcactct ggatcaggat acgaggctag tcgacgatta ttttgaacta
     7381 ttgcgtaaag taacctttac agatgaatgt gttgctgcag ttccgatgat tttttctggg
     7441 agccgacaag tttctcccct atattcagat cgttatatca atcgtacagc tcaagcagta
     7501 gaagttgaag gcccaacagc tgaacgaatc atggcaatca attcaggtgt tgcctggtct
     7561 gtcaaatttc taaaaaagat tggcgggttt aatcctgctt tttctttaga ttttttggat
     7621 cattggctgt tttggaaagt ggatcagttg gaaaaaactg tggagatcct tcctgtacga
     7681 atggaacatg atctttcggt tttagactat gaaaaagttt caagtaatcg ataccgttct
     7741 attttacaag cagaaaatcg attttatcat gaatatgatc aaaggcattt atccagtcac
     7801 cgacgacaat tattgttaag aacagctaaa cagttcttga ccgtcaaaaa ccggcatatc
     7861 tggcggatga ctttgcgctc atatgttaag aattggaagg tgtgaattcg tgcgttcggt
     7921 ttgtatagct acctttaatg gaggcagata tttaagagag cagttagata gtattttatc
     7981 tcagctttca gcagaagatg aagtagtcat ttctgatgat gggtcagtag atgatacatg
     8041 ggatatatta actgaatacg cagaaaaaga taagcgaatc cagctttttt cggggcctcg
     8101 tcaaggattg atcgctaatt ttggttatgc gatccagcag acgaaaggag atctaatctt
     8161 tttggcagac caagatgatg tttggttgga gacaaaagtg aaaaagatca ctgactattt
     8221 tgagcagaat ccggatcagc tagtagtagt ttctgattta atcattgtgg acagcgagct
     8281 gcaagagatc catgattcgt atttttcgtt tcgaaattcc aaagaaggat ggcttaataa
     8341 tgtgataaga agtcactata ttggtgctgg tatggctttc cggtcttctt taaaacccga
     8401 tattttgccg atcccgtcag aagtgccgat gcatgatatg tggattggtt tgctgggagg
     8461 aaagaagact gggttcctaa gagaaccgtt aactctgtac cgaagacatg atttaaacgc
     8521 gagtgagatt gaaacacatt ctaaattacg gcaaaaaatt tcttggcgtg ttcatctgat
     8581 tacggcgttg atcaaacgaa aatggtttaa acgttgataa aagatgttta aatccttaat
     8641 atttccatga caaatataac aattgatgag ataataacaa agtgaaattt gaaatcaaaa
     8701 aggagagcag atatccatga agggaatcat tcttgctggg ggcagcggaa cacgtctgta
     8761 ccctttaaca aaggcaacat cgaaacaatt gatgccgatt tatgacaaac caatgattta
     8821 ttatccaatg tctactctga tgttggcggg aatcaaagaa attctgatta tttcaacgcc
     8881 acaagataca ccacgtttca aggaattatt tggaaacgga aatgatttag gactgcagat
     8941 cgaatatgca gtacaagaaa gtccagatgg attggcacaa gcgtttatca ttggagaaga
     9001 atttattgga gatgatagtg tctgcttggt attaggggac aatatttact atggtggcgg
     9061 tctgtctaaa atgttgcaac gagcagcttc aaaagataca ggcgcgacag tatttggcta
     9121 tcatgtcaat gatccagaac gctttggtgt agtagaattt gatgagaata tgcaagcttt
     9181 gtcgattgaa gaaaagcctg ctcagccaaa atcaaattac gctgtaactg gcctttattt
     9241 ttatgacaat gaggtaattt ctatcgctaa aggtatcaaa ccttcagaac gtggagaact
     9301 agagattacg gatgtcaata aagcatattt ggataaagga aaactatccg tagaacttat
     9361 gggacgcgga tttgcttggc ttgatacagg aacacatgaa tcattgcttg aagcatctac
     9421 ctttatcgaa acaattgaaa aacgtcagaa cttaaaagtg gcttgtttgg aagaaattgc
     9481 ttaccgtatg ggctacatcg ataaggaaca attgctggcc cttgcacagc cattgaaaaa
     9541 aaatcaatac ggtcaatatt tattgaattt ggccgctgaa taaggagtcc ttacagatga
     9601 aagtaacaga tacaaaatta acagatgtca aaatcgtaga aatggacgtc tttggcgatc
     9661 atcgcggttt cttcacagaa agttattcaa aagcaaaatt tgcagaacat ggattgaaat
     9721 atgactttgt tcaagacaac cattcactat cagctgaagc tggtgtcctt cgggggcttc
     9781 atttccaaaa aggagaagct gcacagacaa aattgattcg ggtagtcact ggagcagttt
     9841 tagatgtagt agtggatatc cgcaaaggta gcccgactta tggtcaatgg gacggattta
     9901 tcttatctga acataatcat cgacagttgc ttgtacctaa aggatatgcc catggattcg
     9961 ttactttgac accaaatgtg aacttccttt ataagtgtga taattactat aatgcagaag
    10021 cagatggggg catcgcattt gatgatccag atttagcgat tgattggcca attgatccat
    10081 caagagcgat tatgtcggaa aaagatcacc gtcatccgac attaaaagaa tttgaagcag
    10141 aaaatccatt tgtttacgga gaaatctaag aattaaggag aaattttctt atgaaaaaca
    10201 tcattgttac cggaggtgcc ggattcatcg gctctaattt cgttcattac gtggtaaaca
    10261 atcatccaga agtgcatgtc actgttttgg ataaactgac ttatgcagga aacaaagaga
    10321 acttggctgg acttcctagc gatcgcgtag aattggtcgt aggagatgtt gcagatgcag
    10381 aattagtgga ccgtctagta aaagaagcgg acgcagttgt tcattacgcg gcagaatccc
    10441 ataacgataa ttcattaaaa gatcctttcc catttgtaca aacgaatatc attggtacgt
    10501 atacactttt agaagcttgc cgcaagtatg atgtacgcta tcatcacgta tctactgacg
    10561 aggtttacgg cgatcttcca ttacgtgaag acctgccagg acatggggaa ggcgaaggag
    10621 aaaaattcac tgcggaaaca ccatataatc cttcaagtcc atattcttct acaaaagctg
    10681 gttcagatct gttagtaaaa gcatgggtgc gttcttttgg cttacgtgca accatttcaa
    10741 actgttcaaa caactatggt ccttatcagc atattgaaaa atttattcct cgccaagtaa
    10801 cgaatatctt aagcggtatt cgtccaaaat tatatggcga aggaaagaat gttcgtgact
    10861 ggatccatac gaatgatcat tcttcagcag tgtggctgat tttgacgaaa ggcagaattg
    10921 gcgaaaccta tctgatcggt gcagacggtg aagaagataa taagaccgtg atggaattga
    10981 tcttagaaat gatgggtcaa ccaaaagatg cgtacgacca tgtaaacgac cgtgcaggac
    11041 acgacttgcg ttatgcaatc gattcgacaa aattacggga agagttagga tggaagcctg
    11101 agtttacgaa cttccgtgat gggttggctg acacgatcaa atggtacgaa gaacatgaag
    11161 actggtggaa aaaagaaaaa gaggccgttg aagcagcata tgccaaaaat ggacaataat
    11221 acagagaaaa tgtcactgta ttaatggaaa aacacatgtg aggcaggcgc tcgcctgtct
    11281 cttttttaaa ataaaaatca aggagcggat aatcatatgt ttttattaac tggaggaaat
    11341 gggcaactag gtacagaatt gcgccattta ttagatgaaa aaaatctgca atatgtttca
    11401 acagatgcaa aagacttgga tatcacagat gccgagaaaa caatggctta catcacagaa
    11461 ttaaaacctg atgttatttt ccattgtgca gcttacacag cagtagataa agcagaagaa
    11521 gaagcaaaag aattagatga gaagatcaat gttgatggta caagaaatgt agcacttgca
    11581 gcaaaagctg tgaatgctca attcgtctat atcagtacag actatgtttt cgatggaaaa
    11641 aacaaaaatg tagaatacga agtagacgac caaacgaatc cactaaatga atatggtcgt
    11701 acgaaattat taggcgaaaa agcagtagaa gagattttag aagactatta tatcatacga
    11761 acttcttggg ttttcgggat ctatggacac aatttcgtct tcacgatgca acgcctagcg
    11821 gaaacccgcg acaaactgac tgtagtaaat gaccagtttg ggcgaccaac ttggacacgt
    11881 actttggcag aatttatggt atatgtcatc gaagaaaaag caccatttgg tatttatcat
    11941 ttgtcgaacg aaaatagctg ttcttggtat gaattcgcaa aagaaatatt gaaagataca
    12001 gatgtagaag ttgcacctgt tacgtcagaa gaatttccgc aaaaagcaac acgtccgcag
    12061 tattcagtca tgagcctgaa aaagacagaa gagttaggat ttgtcatacc aacttggcaa
    12121 gaagcacttg cgcaaatgct ggaagctgcc aaaaaaatga ataagtaatt tccttttatc
    12181 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    12241 ccaaaagcga accaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    12301 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    12361 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    12421 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    12481 caagctgaag aggactttcc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    12541 atcattgcgt ataattctcc tgcaaaggat attacggtta tcggaaaacc attcaactgg
    12601 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    12661 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagagaaa aaaagctcta
    12721 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    12781 catgtgggcg atttgatttt ctttactttg ggactgatga ctgcttttta tcattatttc
    12841 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    12901 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    12961 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    13021 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    13081 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    13141 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    13201 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    13261 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    13321 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    13381 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    13441 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    13501 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    13561 tatgcgatta cgcattcgaa aatagataat tatttttcgg attttgaaac catcgcagtt
    13621 gctgtgttgg gaacagtgat tttagcattt gcattatttg gctggagacg cttgttttac
    13681 ctcgcttttg ccgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    13741 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    13801 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    13861 aaatccttca atacagtacg cttttatcca gatatagctg catggaagga aatcgatccg
    13921 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    13981 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    14041 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    14101 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    14161 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    14221 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
    14281 ggagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    14341 tttgttgctg acgctagtct tttttgtttg gacagcaggg ttaggttttc cagcaacttt
    14401 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    14461 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    14521 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag atttactgtt
    14581 tcctttgatt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    14641 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
    14701 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
    14761 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
    14821 atttgccagt tcttttgaac gatggtatgt acgaatcatc ggtgcagtag cagttgttgc
    14881 agcttttctc gggatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
    14941 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
    15001 ttacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
    15061 agtcaatttg tttaccggtc gagaagatat ttggcataaa ttctatcaga cgctaggaga
    15121 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccaacgag gaaaccaatt
    15181 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    15241 gattgctact ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    15301 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    15361 ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt
    15421 tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga
    15481 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    15541 gatccaacgc tatcattgat aggcacaaaa tacggctctt tgtcaataag gactgatggg
    15601 ttgtacaaaa tcaaatctgg gtcagaatga acctcattct ggctcagatt ttttgttatc
    15661 ttactttgat taattgattg atcaaaaaga ttctaat
//
LOCUS       DACECB010000065        14632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECB010000065 DACECB010000000
VERSION     DACECB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.065"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IWO04_002182"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IWO04_002182"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0213764.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IWO04_002183"
     CDS             951..1256
                     /locus_tag="IWO04_002183"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0213765.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IWO04_002184"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IWO04_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0213766.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IWO04_002185"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IWO04_002185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0213767.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IWO04_002186"
     CDS             4416..5354
                     /locus_tag="IWO04_002186"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0213768.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IWO04_002187"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IWO04_002187"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0213769.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IWO04_002188"
     CDS             6725..7465
                     /locus_tag="IWO04_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0213770.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IWO04_002189"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IWO04_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0213771.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IWO04_002190"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IWO04_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0213772.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IWO04_002191"
     CDS             10608..11258
                     /locus_tag="IWO04_002191"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0213773.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IWO04_002192"
     CDS             11259..11897
                     /locus_tag="IWO04_002192"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0213774.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IWO04_002193"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IWO04_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0213775.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IWO04_002194"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IWO04_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0213776.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..>14632
                     /locus_tag="IWO04_002195"
     CDS             13674..>14632
                     /locus_tag="IWO04_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213777.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSAT"
BASE COUNT         4913 a         2716 c         3182 g         3821 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca ac
//
LOCUS       DACECB010000066        14531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECB010000066 DACECB010000000
VERSION     DACECB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.066"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1183)
                     /locus_tag="IWO04_002196"
     CDS             complement(44..1183)
                     /locus_tag="IWO04_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0213778.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQHFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(1297..1881)
                     /locus_tag="IWO04_002197"
     CDS             complement(1297..1881)
                     /locus_tag="IWO04_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0213779.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(2008..2439)
                     /locus_tag="IWO04_002198"
     CDS             complement(2008..2439)
                     /locus_tag="IWO04_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0213780.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2457..2777)
                     /locus_tag="IWO04_002199"
     CDS             complement(2457..2777)
                     /locus_tag="IWO04_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213781.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            complement(3208..3387)
                     /locus_tag="IWO04_002200"
     CDS             complement(3208..3387)
                     /locus_tag="IWO04_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213782.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            3468..4214
                     /locus_tag="IWO04_002201"
     CDS             3468..4214
                     /locus_tag="IWO04_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0213783.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            4229..4432
                     /locus_tag="IWO04_002202"
     CDS             4229..4432
                     /locus_tag="IWO04_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0213784.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            4448..4786
                     /locus_tag="IWO04_002203"
     CDS             4448..4786
                     /locus_tag="IWO04_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0213785.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(5041..5706)
                     /locus_tag="IWO04_002204"
     CDS             complement(5041..5706)
                     /locus_tag="IWO04_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213786.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            5761..6459
                     /locus_tag="IWO04_002205"
     CDS             5761..6459
                     /locus_tag="IWO04_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0213787.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEKISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDTVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            6637..6977
                     /locus_tag="IWO04_002206"
                     /pseudo
     CDS             6637..6977
                     /locus_tag="IWO04_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6970..7641
                     /locus_tag="IWO04_002207"
     CDS             6970..7641
                     /locus_tag="IWO04_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0213788.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            7647..8333
                     /locus_tag="IWO04_002208"
     CDS             7647..8333
                     /locus_tag="IWO04_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213789.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            8340..9191
                     /locus_tag="IWO04_002209"
     CDS             8340..9191
                     /locus_tag="IWO04_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0213790.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            9203..9472
                     /locus_tag="IWO04_002210"
     CDS             9203..9472
                     /locus_tag="IWO04_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213791.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            9634..9936
                     /locus_tag="IWO04_002211"
     CDS             9634..9936
                     /locus_tag="IWO04_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213792.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            9933..10094
                     /locus_tag="IWO04_002212"
     CDS             9933..10094
                     /locus_tag="IWO04_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0213793.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            10091..10396
                     /locus_tag="IWO04_002213"
     CDS             10091..10396
                     /locus_tag="IWO04_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213794.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            10396..10710
                     /locus_tag="IWO04_002214"
     CDS             10396..10710
                     /locus_tag="IWO04_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0213795.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVERYREILNEYFE
                     ED"
     gene            10711..10899
                     /locus_tag="IWO04_002215"
     CDS             10711..10899
                     /locus_tag="IWO04_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213796.1"
                     /translation="MFNLTLVSIHFLFYITIFVAILINTIKSGFLTPIGTILTLASFV
                     TSCIIQLKYIKENEKWTN"
     gene            10887..11198
                     /locus_tag="IWO04_002216"
     CDS             10887..11198
                     /locus_tag="IWO04_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213797.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            11195..11413
                     /locus_tag="IWO04_002217"
     CDS             11195..11413
                     /locus_tag="IWO04_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAQ0213798.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            11552..11908
                     /locus_tag="IWO04_002218"
     CDS             11552..11908
                     /locus_tag="IWO04_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0213799.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERVELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDKQTNWSQKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            11868..12113
                     /locus_tag="IWO04_002219"
     CDS             11868..12113
                     /locus_tag="IWO04_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213800.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            12110..12529
                     /locus_tag="IWO04_002220"
     CDS             12110..12529
                     /locus_tag="IWO04_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0213801.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKGGKVNSIEYWKTPSELKSY
                     HVRNLVLMQSTGLKDKNGVEIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYQNSELLEEQR"
     gene            12526..13143
                     /locus_tag="IWO04_002221"
     CDS             12526..13143
                     /locus_tag="IWO04_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010733969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0213802.1"
                     /translation="MNKQELINELTKYVERYEGATDEYYQGKSSAYEVALKLAKKLGL
                     DEPQKPVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYYVRLPYEVWDEEAAELKPEYLYLHYEIT
                     SDETRMFPTKEPRKGFVAKLDELTIKSADERFWPFAVPVEEVAEG"
     gene            13212..13406
                     /locus_tag="IWO04_002222"
     CDS             13212..13406
                     /locus_tag="IWO04_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213803.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            13437..13733
                     /locus_tag="IWO04_002223"
     CDS             13437..13733
                     /locus_tag="IWO04_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213804.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWTAKSKENLAVARLVDMELINDK"
     gene            13809..14225
                     /locus_tag="IWO04_002224"
     CDS             13809..14225
                     /locus_tag="IWO04_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0213805.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         5183 a         2238 c         2901 g         4209 t
ORIGIN      
        1 gatacagggg tttgttttgt gtttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagca ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagtg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tctactctct
     1201 ttctctgata caataggcac tataaagaag cctactgtat aggtttgttt ttcttagaac
     1261 acgcttgctt tggacggtgg gcgtgttttt tatttattat acagttgctt taaaaatagc
     1321 tgctttttca aatgatacta aaggagagaa gtggatttct atttctccct tttggtttaa
     1381 cgtgaaatga gcagttacat ccatttgttt tcctggagca acagatccca ttgtattttc
     1441 gttggcatat gtctctgatt ttttgtcatc tggtccatat acttccacat ctgtacctac
     1501 agggatatct gaatcgccat cattttttac ggtataagta atttttacta cttgtgcagg
     1561 ttggttttct tcaaattgat ttctttcatc agttagttct acactattta gtgtatattc
     1621 agcatcacca acttttacag tgtcaccaat cttatagaag gtatcgcttt tttcttcttt
     1681 agaagatgaa gcagaggttg aagatttagt tactttttcg ccaccattat cacttgcttt
     1741 attactatct gatccaccat ttaaagcaga accaataatt ataattaaaa ttactgctaa
     1801 tacccaaaac catacttttt tgtaaaaagg tttactaact ttatacattt ttccgtcttg
     1861 acccataact ttttttgcca tttaaatatt cctcacttct tgttataata tatttgcgat
     1921 ctcagaaatg aggtatgagt ccgtgtgcca gcacgggctt ttttaatgtt ttgctgaaat
     1981 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
     2041 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
     2101 atactccata aatttttcga gattaaactg agattcatca gtcagttcgt tctcaatata
     2161 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
     2221 agttgtagaa gtatcgtaca aaactgaata ttcaaaatga gaagcggtga aatgcgcaag
     2281 ttcatgagat aaatgaaagg cttctgcagt ttcgccatat agattttcat tcaaaaaaat
     2341 gattctaggc ttcggataat agaagccagg ctctttcatt tccatgtaaa ctactttcaa
     2401 attgtattcg ctcaacattt ctttcaattt caaatacata caacccatca ctccaactat
     2461 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
     2521 tcttttccat caaaagaaaa aacagtatcg ttttctgata aatccacatg tttaggggtt
     2581 tctttttctt cgcgccccag aagatagtct acagagacat cgaaatagtc agcaatttct
     2641 tgcaaacgtt ctgtagaggg gtttgagttc ttaagtctat ataagacgtt tttaccatag
     2701 cctaggtctt cttcaacttt atttaatgat tttccctgtt ttttcgctaa ctcttttatt
     2761 ctctcaaacg ttatcatatc aatattcctt tcgttcaaga aaaaatattt aactttattt
     2821 gttaaaattc gttgactatt ttaacaaaag gtgttaatat cattcttgta aacaagttta
     2881 acaactaaaa agacaacaaa aaacactatt gattaataaa tgccaaccgc caagaaagct
     2941 tttaaaatca atgtttatat gtcttattta actatgttct gattttaaca ctgtatgtta
     3001 atttagtcaa cgcagttttt ttaaaagttg ttaaatttgt ttacgaatat aaaagaaagg
     3061 agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact
     3121 gaaacaagca aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc
     3181 agccatagca gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg
     3241 accatagata acagcataat gaatatctaa ataagcttga gcaatttctt ttggcgaaat
     3301 tgattcgcct ttagtagata cttgtgattc gtgatttagc catgcaacaa ctaaatccgc
     3361 agcgatttca tcagtttttt tattcatact tattcaccac cttatgcatt atttcagtag
     3421 accacttact gataagaaaa ttatatcaaa gaaaggaatg aacaaaaatg aacacaccac
     3481 aaatttttag ttttgaacaa catgaagtaa gaacgtttct agaaaatgac attccgtatt
     3541 tcgtagcaaa cgatgttgcc aaaactttgg gatacaaaaa tcccagtaaa gccaccaatg
     3601 atcattgtaa aaaatcaatt gaaacatggg gtaacgattc gttaggtcgt cgtcaaaaat
     3661 tcaaagttat tccagaatca gatgtttacc gcttgattat taaatcgaac ttaccaagcg
     3721 ctgaaaaatt tgaggcctgg gtaatggaag aagtccttcc aacaatcaga aaaacaggta
     3781 gctattcaaa cgtacctcaa agttttgcac aagcattgcg tttagcagca gatttagaag
     3841 aaaagaacca attactcgaa caacaaattg ccgagtacga accaaagatt agctacttag
     3901 atacgattct ttcatcgaca gatacggtag ctacttctca aattgcagct gattacggaa
     3961 tgtcggcaat tgctctaaac aaattgctaa acgagttagg tgttcaacat aaagttagcg
     4021 gacaatggat actttaccga aaacatatga accaaggata cacaaaatcg cacacaagtg
     4081 agattccgaa agccgatggc ggcactaaag ttgtaatgaa taccaaatgg acacagaaag
     4141 ggcgagtgtt tatttacaac ttattaatcg cagagggcta ttaccctcaa atggatttag
     4201 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
     4261 tagagaaaaa atcaaagaat atgtcgaaag ttcgaagttc ttaacttaca ccatgcttgc
     4321 tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
     4381 tgcagaagca aatagaatca tagattcgat tattactatg tacgaattgt aaagggagga
     4441 acagctaatg caatatctag aagcgaaaat cccagttcca gaaggctatg taattatctc
     4501 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
     4561 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
     4621 cccaagatat agaaatatta tcgatatcga aaaaaataaa aatggatttg tctattatcc
     4681 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
     4741 aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtat
     4801 tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcagacaa gcgttgatag
     4861 gaacgattgt ttacatggtt tggattttta tggggctgga tgaagctgag tacaaaacaa
     4921 aaaagccagt cgggagggac tgactaatga aaaaaagttt aatgactata aacgaaaaac
     4981 aattgaaaga aaattttaat gatctcatca aagaatttgt aaaagaaact ggagaatttc
     5041 ctaatcaaat tcatctagtt gcggaggggc atagccagta tcaagctgtg aaatttgaga
     5101 tgaagaaaca aatctattga atttagcagt acctctactt gtaagaacat ttattgtaat
     5161 gtttttttcg actaaagatt catcgtatct aaaaaccaac gtagtaattt ttgactcata
     5221 cggtggaatg gtaataggta atgacaacgg aggaaatttt gatagatcag cacctttttt
     5281 taaagattta ccagaatacg cacgtatacc atgtggaagt tcgtcataca ttgattttac
     5341 cgttacagaa taattatcac caatcattgt aaaagcattt aaatgatcag gaattccaga
     5401 aatcgaaaac tcaataattg taacaggtaa agaactgtta tttgttaaca atacagaatc
     5461 tataatcctg aatttatttt gatggtaaac atctggtttg gaatcacttt ctctatctat
     5521 caaccattct tcgcattcag cgaagaaatc caattttaaa ttaaattttt tgttttttct
     5581 atctgtatag gagatataaa aagcaaatgt tgaaaatact agagacgtaa taggcaaaat
     5641 aaaatcttta atggttttaa caatgttatt ccagtcaaat tgtttaataa agtcaataag
     5701 actcaattta taccaccacc attttttatc tacattatat caaagaggag agaagaaata
     5761 atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagttt gcaagttgca
     5821 gaaagttttg agaaaaagca tcaacatgtt ttaagagata ttgatgcact gaaaaaagat
     5881 gtgtccaatt ttggacagat gttttctgaa ggtaatgaac cagattcata cggcagaaat
     5941 cgacgagttt tcttcattag tagagatggt tttttcttgt tggctatggg ttttacagga
     6001 aagaaagcta tttacttcaa acaaaaatac attgaagcat tcaacgaaat ggaagatgtt
     6061 attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctacca actagaagaa
     6121 atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat tagtgggcaa
     6181 caagagtttg aaattaaaca aaaaggaaat ataaaagtta tggaagttct agggggtaaa
     6241 gaaagccgag cttatgaaaa aatcagcaaa aaagtattct caaaattttg gtctgaattt
     6301 aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt cgatgatact
     6361 gtttcattta ttgaaatgtg gttaccagaa actgcgatcc gcatggaaat cgatcaactg
     6421 aacagacaac aaagactttt cggtgatgaa aatgaataga gctgaagcgc taagaatcgg
     6481 gacggtaatt gctaatcgct ggtggagaca caataaacca atcatcctaa gccaacaaca
     6541 tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag
     6601 tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact ttccactcta
     6661 gatcaatatt tgactgatcc tagttgggca aatcgaatat caaggaaaca aataatcgaa
     6721 aaatcagacg aaatcttttg acggatgaag aactagcatg tgatcaagac gatttgggaa
     6781 attttgtgag tatttgggat catgtctatc ttatccattt atcgaggaag tccaaaaaac
     6841 ctgaatatat ctatgtcatc gaagatggct tgattgatgc gctagaagag tatgacagag
     6901 ataacttgat tgatatctct tattacggat caggtaagaa atacattgct gaaatggagg
     6961 cagaatttga tgagtgaaaa caaagggaca acgacttttg aaaaactttt tagtcgcaag
     7021 ttaaataaaa tactcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg
     7081 gagattatga aaaagaatga tccacaggca acggtaacta ttaatgagta taaacactac
     7141 agggttgttt ctggaactca tcaagacttt cttgttgagg aatataaacc ttttcttatg
     7201 gatgaaacag ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccgag
     7261 ttatttcctg ttttagatta tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc
     7321 aataactcat tgaagcgatg ctttgtgaaa gcattggctc tacacggact gggattatat
     7381 gtatttcaag gggaagatat tccaacacca cctagaatcg atacgaagaa attaaacatg
     7441 ctagagacga ttctagaagc tttcaatgag cagatgggta aagatatgac caaaacctta
     7501 atcgaatatg ttaatgagca gacagataaa ttagggctct tagctgataa cgttgaaact
     7561 attgaacagt taagctatga gcaatgtgcc ttgatggagc gagcaatagc agctaagaga
     7621 aaagaattag ataagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg
     7681 ttctgaaaaa gttcaaagga aaagacatag gtgcaaccat caatgaggaa gtgaacattg
     7741 atcgactaaa gacgatgtat gacggctacg atggtgatcg agttattgaa attcgtttta
     7801 ttgatcctag acgtttcaca gtacagcaac gaaacttcat ctatgcactc ataggcgata
     7861 ttttcatcga tacaggcatg ccaacggact tctggaagga attcttttac ttccgctttg
     7921 aaggtgtcac agggcgcaaa ataagcctca aagatgaatc gaatacgact gtgagtgatg
     7981 ctaacgtctt agcaaatatc atcttagatt tcatctttga acatcatatt cctttcaaag
     8041 aaggctatga gattttacca gcgaatcaag aatattactt ctacaaatgc atcacaaaaa
     8101 gagtctgttg catctgtggc aaaacaggag ctgatatcga tcactttgac aaagcgctag
     8161 gaagacgaaa gcgcaaagaa gttgatcatt cagagtacac atttgcagca ctctgcagaa
     8221 tccatcacac ggagaaacac aagataggtg tgatcaattt caaaaataaa tatcaaatca
     8281 aagggatcaa gttaaaccag gaaacaatta agaaacttag aataggagga taaaaaatat
     8341 tggctgacaa caaacgatac tactatttaa aactaaaaga gaactttttc gatagcgacg
     8401 agatggttct tttagaaagt atgccagatg gatacattta ttctaatatt cttctcaaac
     8461 tttatttaag aagtctgaaa catgaaggca aactaatgtt taatgacagg attccattca
     8521 attctacaat gcttgcgact attacaagac actctgtggg agttgtagaa aaagcagtac
     8581 aaatatttcg tgatttacaa cttattgagg tattagataa cggagcaatt tatatgtctg
     8641 atatacaaag ttttattggg aaatcttcaa ctgaagctga taggaagaga aaatacagaa
     8701 aagaaattga ggaagcaaaa cagaatttaa taacaagtgg acaaatgtcg gacaaatgtc
     8761 cggacaaaac tacaccagag ttagagatag agttagagaa agatatagag ttagagaaag
     8821 atatagagaa tgtaacgcct tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg
     8881 gagagtataa aaatgttctt ttgtcagatg agcagatgga gaaactcaaa atagaattcc
     8941 ctaatgacta tcaagaacga atagaacggc tatctgagta ttgtgaatca tctggtaaga
     9001 cttataaaaa ctatttggca actattcgaa gctgggcaag aaaagaaaaa agtgaatcta
     9061 agaacgcaag cagtggatac aagcgcacag gaagacgaga gaagcttcct gagtgggcaa
     9121 tcgaccaaga agcctatctc aagaaaaaag cgctagaacg agctaataga caatcaaaag
     9181 caccattcta agaggtggga aattgaaaat cgattatcta gaactaatta atgaaatagc
     9241 aaagtataaa actggtgagg aaatagaaat cctgagagac gtatatgatc aactcgaaga
     9301 agctggaatc gaaggaatta agaatgatcg ttcgagttgg agtaagctca gatactattt
     9361 cgcactttat atcgatacaa cacaattaag aaatttagca tataccaaat tactatttgt
     9421 tgattgtgtt aaaggcttgc aaaaacatct taatgaactt gagcaggtgt aatcagatgg
     9481 acctaaagac atttacagca cagattgaac taatgcatca agaagcttta agacaaagtg
     9541 tatcgtacga agacaagtgg ctcaacacgt tccacggcgg acgtgagagc gcacttgatc
     9601 aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc aatgcagcga
     9661 attatcgaat tgacttattc agaagattgg caaaatgaca aagaagctgc ttcagaagtg
     9721 atgaggcttg gaagagcgat gtgggcagac aagagcaaca agccaagacc acgaaaaatc
     9781 gcaatttggc acggtgacaa acttctagtg atagggacag ctgaacagtt agcaagtctc
     9841 acaggcttgc acgagaaaat cgtgaggaaa agagcaaggt gtggctacac agacgctaag
     9901 aagagaacgt ttagatacgt ggaggaatca tcatgacaac agaagaagtg attcaaatgc
     9961 gtattcgaag ccttcagcgt gagattgacg atctggaacg aacaaaggca gtgatggtca
    10021 atgaaacggc gagaaaggca atcgatttgc acatagagaa tttaagaagg gaaatccatc
    10081 gattggagga atgagcgtgg ataagaaagc agcaatgaaa agaattgctg aattaaccaa
    10141 gtcagaatct tggcaagaag acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc
    10201 aatgtggact gaaaagccca aacggagaac gccgagaaaa attgcaatct ggcatgatga
    10261 ccgaatttta gtaacaggta ctgctgaaca gttatctgaa attactggat taagcaaaaa
    10321 cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac agtttaggta
    10381 tgtggaggag agataatgga tctcattaca caatacagtg acatcatcct caagaaaatc
    10441 atgatgaaga ttcagaaaga taaaaaatca aaagaacgag ctgaattagt taagttagaa
    10501 atggctgaaa caggagcagg agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt
    10561 gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa
    10621 acaaattgga gcaagaaact tcatcaagat cgtttcaaat ttgtagaaag gtatagagag
    10681 atactgaatg agtatttcga ggaggactag atgtttaatt taacgttagt gtcaattcac
    10741 tttttattct atataacgat atttgtagct atattaatta atacgataaa aagcggtttt
    10801 ttaacaccaa tcggcacaat tttaacatta gcttcatttg ttacaagttg tattatccaa
    10861 cttaaatata taaaggagaa tgaaaaatgg acgaactaat cacaaaagta gagcagtggg
    10921 caaaagacaa gggactggat caagcggatc caaaagcaca gtttttgaaa gtagctgagg
    10981 aattcggaga aattgcttca gcgatggcaa gaagtaatga tgagctattt aaagatagcg
    11041 taggagacgt tatcgtcacg ctgattatcc tttccatgca aaaagggaca aacatacaag
    11101 agtgtttaga aatggcatac aacgaaatca aagggcgcac aggaaaaatg gtagatggtg
    11161 tattcgtgaa gtcgagtgat ttggaggaca gcaaatgata ggtttaacaa tattttggtg
    11221 tgcgattggt ggtttactag catttctagg atactacctc atctgtgaaa gtagaaaagg
    11281 aagaaaatat acagtgacag gtattgtcct gttaattatc ggaattctag ttgcagttgg
    11341 agcagaactg aatctttatg taaatggtac acaagaagat ttggtgcgat ttatgttttg
    11401 gatgaagaat taggaggaaa aaaatgaacg aaaacaaatt aatcaaatta ggtgtagcag
    11461 gagcagcata tctcgtgtat gcgctccttt cccaaactga tgacaaagaa gatgacaaca
    11521 acgatgaaat gaaattttag gaggagaaat aatggatctc attacacaat acagtgatat
    11581 catcctcaag aaaatcatgg caaagattca gaaagataaa aaagcaaaag aacgagtgga
    11641 attagttaag ttggaaatgg ctgaaacagg agtaggagtg cgaagtagca ggcattggaa
    11701 agcagcagca aacattgaat tttattacaa cgaaattcaa aaagggttcg atcagatgcg
    11761 tgagcttgat aaacaaacta actggagtca gaagcttcat caagatcgtt tcaaatttgt
    11821 agagaagtat aaagaaatat tagaagagta tttgaggagg acagcaaatg ataaaaaagc
    11881 tcattcaatt cagcatggat ttatatgata tcgaatcagg agcaacagta tctgtggagt
    11941 ctgatcattt aatcataagt tttgctgacg aacgtcaaat tatcatatgg gtagttgacg
    12001 atatgctata tccagaaatt gtacatgatt ttgaagaatc aaaagcggtt gagtttgaaa
    12061 tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga ggacagcaaa tgataccgaa
    12121 gtttagagcg tgggataagc gagaaaacac aatgagagat gtagctgtct tacattttac
    12181 taaaggcggt aaggtaaaca gtatcgaata ctggaagaca ccttccgagt tgaaatcata
    12241 tcatgtacga aatttagtcc tcatgcaatc tactggcttg aaagataaga acggtgtgga
    12301 aatatttgaa ggggatatag tattagtcag cgtgcgaaat ggcttcgatt acttagataa
    12361 taaagtttgt attgtcaaaa attcaataga ttattccgga ttagtttgtg ccacggttga
    12421 tgaagattta gagtatcaaa tttttaacac agagctgttt gaagaataca cgtatgaagt
    12481 catcggaaat atataccaga atagcgagtt attggaggaa cagcgatgaa taaacaggaa
    12541 ttgattaatg aattaaccaa atatgttgaa cgatacgagg gtgccacaga tgaatattat
    12601 caaggaaaaa gcagtgctta tgaggtagca ttgaaattgg caaaaaaact agggttagac
    12661 gaaccgcaga agccagttat tccacaactc gtggctggtt ggcttgagaa atctacggac
    12721 ccttttacaa aagcagaaaa aatagcgtat ttaatcaaat ctaaagatgg tgattcatat
    12781 tatttctgtg attggtttgt acgagatggc atattgacgc aagaacaagg agaagaatta
    12841 ctcgcttggg caacaagaca atcatatgaa acactattga gcctatacaa cggctacgag
    12901 gttgagaaag agccgttgta ttatgtgaga ctaccatatg aagtttggga cgaagaggca
    12961 gcggagctta aaccagagta tttgtatcta cattatgaaa tcacaagtga tgaaactaga
    13021 atgtttccta caaaagaacc tagaaaaggt tttgttgcaa aattagatga acttacgatt
    13081 aaatcagcag atgaaagatt ctggccattt gctgtgccag tggaagaggt ggcggaagga
    13141 tgagtgaaga tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa
    13201 ggggtgtagt catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt
    13261 tcggagacgt cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga
    13321 ttaccgacat tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag
    13381 tagccatttt agtgacttgc gagtaagtaa ttgacagcta aagaggagga agcggaatga
    13441 aactaaaaga cggattttac gctagtagcc acggcatcgg cggtttaatg ctagatatgc
    13501 cgactaagaa tcctaaaaca cgcaagaaat caaaattcaa agtcggtgac atggttcgct
    13561 gtgaagcaga aggattcatt tatccctttc gtggatatgt agagcatctc tataatcact
    13621 cagcaatcat tcgcattgaa aacacaatgg aatgtgataa atggacagct aaaagcaaag
    13681 agaatttagc tgtagcgaga ttggtggata tggaactaat caatgacaaa taaaaaagcc
    13741 ggatcgctcc gactgattca ataaatccaa cacatttatt atatcacata aaggagcggt
    13801 ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt aatgcgagag
    13861 atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg atagatatta
    13921 agtcgccgat tattacggat atgccgaagg caccgaagca cggcaataag gcagaagacg
    13981 cgatcattca gatgatggat atagaagcgg agagagacgc gattttagcg gctttgatgg
    14041 ctcttagtct gattagtcgt cagatactct actacagctt ctgtgacgta aataagcact
    14101 ctaattatga aataggtcaa ttgatacgag gatacggaga gaagaatgta gagaagctga
    14161 aatccatcgc attgatcgaa tttgctgaag catacaaaaa aggcgtgtta gttcagtatc
    14221 gttgattttg tagggttttt gtagggatag tgtagggttt ttgagtggtt taacgtgata
    14281 ttatgatagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
    14341 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
    14401 ggatgtgaat ttcaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat
    14461 taatttataa aaagtaacaa ataatgagga ggtattataa atgaattcac aaaatttgaa
    14521 agatttaaat a
//
LOCUS       DACECB010000067        14118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECB010000067 DACECB010000000
VERSION     DACECB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.067"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..556)
                     /locus_tag="IWO04_002225"
     CDS             complement(<1..556)
                     /locus_tag="IWO04_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0213806.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="IWO04_002226"
     CDS             complement(572..1051)
                     /locus_tag="IWO04_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0213807.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="IWO04_002227"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="IWO04_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="IWO04_002228"
     CDS             complement(2098..3204)
                     /locus_tag="IWO04_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0213808.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IWO04_002229"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IWO04_002229"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0213809.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IWO04_002230"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IWO04_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0213810.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IWO04_002231"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IWO04_002231"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0213811.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="IWO04_002232"
     CDS             8175..8873
                     /locus_tag="IWO04_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0213812.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="IWO04_002233"
     CDS             complement(9041..11680)
                     /locus_tag="IWO04_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0213813.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IWO04_002234"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IWO04_002234"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0213814.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="IWO04_002235"
     CDS             13523..>14118
                     /locus_tag="IWO04_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0213815.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DACECB010000068        13945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECB010000068 DACECB010000000
VERSION     DACECB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.068"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IWO04_002236"
     CDS             950..1423
                     /locus_tag="IWO04_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0213816.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IWO04_002237"
     CDS             1432..1659
                     /locus_tag="IWO04_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213817.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="IWO04_002238"
     CDS             2295..2666
                     /locus_tag="IWO04_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213818.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="IWO04_002239"
     CDS             complement(2922..3164)
                     /locus_tag="IWO04_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0213819.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="IWO04_002240"
     CDS             complement(3196..4098)
                     /locus_tag="IWO04_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0213820.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="IWO04_002241"
     CDS             complement(4111..4299)
                     /locus_tag="IWO04_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0213821.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWO04_002242"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWO04_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0213822.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="IWO04_002243"
     CDS             complement(4914..5807)
                     /locus_tag="IWO04_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0213823.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6823)
                     /locus_tag="IWO04_002244"
     CDS             complement(5885..6823)
                     /locus_tag="IWO04_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0213824.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="IWO04_002245"
     CDS             complement(6857..7207)
                     /locus_tag="IWO04_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0213825.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="IWO04_002246"
     CDS             complement(7240..8142)
                     /locus_tag="IWO04_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0213826.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="IWO04_002247"
     CDS             complement(8135..8992)
                     /locus_tag="IWO04_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0213827.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="IWO04_002248"
     CDS             9326..10135
                     /locus_tag="IWO04_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0213828.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="IWO04_002249"
     CDS             10175..10672
                     /locus_tag="IWO04_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0213829.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="IWO04_002250"
     tRNA            complement(11057..11130)
                     /locus_tag="IWO04_002250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="IWO04_002251"
     CDS             11317..11640
                     /locus_tag="IWO04_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213830.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="IWO04_002252"
     CDS             11804..12058
                     /locus_tag="IWO04_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213831.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="IWO04_002253"
     CDS             12128..12373
                     /locus_tag="IWO04_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0213832.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="IWO04_002254"
     CDS             12449..12814
                     /locus_tag="IWO04_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213833.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="IWO04_002255"
     CDS             complement(12875..13438)
                     /locus_tag="IWO04_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213834.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4088 a         2577 c         2304 g         4976 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatga
//
LOCUS       DACECB010000069        13918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECB010000069 DACECB010000000
VERSION     DACECB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.069"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..506
                     /locus_tag="IWO04_002256"
     CDS             132..506
                     /locus_tag="IWO04_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0213835.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IWO04_002257"
     CDS             complement(620..1249)
                     /locus_tag="IWO04_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0213836.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IWO04_002258"
     tRNA            complement(1383..1455)
                     /locus_tag="IWO04_002258"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="IWO04_002259"
     tRNA            1669..1742
                     /locus_tag="IWO04_002259"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            1697..1846
                     /locus_tag="IWO04_002260"
     CDS             1697..1846
                     /locus_tag="IWO04_002260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213837.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2339..3667
                     /locus_tag="IWO04_002261"
     CDS             2339..3667
                     /locus_tag="IWO04_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0213838.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="IWO04_002262"
     CDS             3869..4702
                     /locus_tag="IWO04_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0213839.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="IWO04_002263"
     CDS             4718..5455
                     /locus_tag="IWO04_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0213840.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="IWO04_002264"
     CDS             complement(5610..6578)
                     /locus_tag="IWO04_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0213841.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IWO04_002265"
     CDS             6810..7643
                     /locus_tag="IWO04_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0213842.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IWO04_002266"
     CDS             7677..8516
                     /locus_tag="IWO04_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0213843.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IWO04_002267"
     CDS             8542..9102
                     /locus_tag="IWO04_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0213844.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IWO04_002268"
     CDS             9301..11022
                     /locus_tag="IWO04_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0213845.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IWO04_002269"
     CDS             11187..12329
                     /locus_tag="IWO04_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0213846.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IWO04_002270"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IWO04_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0213847.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4036 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DACECB010000070        13817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECB010000070 DACECB010000000
VERSION     DACECB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.070"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWO04_002271"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWO04_002271"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0213848.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWO04_002272"
     CDS             complement(2261..2512)
                     /locus_tag="IWO04_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0213849.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWO04_002273"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWO04_002273"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0213850.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWO04_002274"
     CDS             3462..3680
                     /locus_tag="IWO04_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213851.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IWO04_002275"
     CDS             complement(3969..4481)
                     /locus_tag="IWO04_002275"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0213852.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IWO04_002276"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IWO04_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0213853.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IWO04_002277"
     CDS             complement(6934..7368)
                     /locus_tag="IWO04_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0213854.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IWO04_002278"
     CDS             complement(7378..8166)
                     /locus_tag="IWO04_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0213855.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IWO04_002279"
     CDS             complement(8163..9104)
                     /locus_tag="IWO04_002279"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0213856.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IWO04_002280"
     CDS             complement(9281..9868)
                     /locus_tag="IWO04_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0213857.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IWO04_002281"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IWO04_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0213858.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11699..12709
                     /gene="gap"
                     /locus_tag="IWO04_002282"
     CDS             11699..12709
                     /gene="gap"
                     /locus_tag="IWO04_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0213859.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12794..13729)
                     /locus_tag="IWO04_002283"
     CDS             complement(12794..13729)
                     /locus_tag="IWO04_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0213860.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3811 a         3095 c         2436 g         4475 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaaga gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgtaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat
    11701 gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat
    11761 caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat
    11821 gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc
    11881 aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc
    11941 aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt
    12001 ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat
    12061 ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga
    12121 tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc
    12181 ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta
    12241 cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg
    12301 ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt
    12361 tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg
    12421 ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga
    12481 agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt
    12541 ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt
    12601 aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg
    12661 atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc
    12721 aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta
    12781 cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt
    12841 tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt
    12901 ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt
    12961 cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg
    13021 cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg
    13081 gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag
    13141 aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca
    13201 tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat
    13261 tgttttctag cactttgttt atccactgta catagtcttt acgctctggc catccaaatc
    13321 caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac
    13381 ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat
    13441 gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt
    13501 tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt
    13561 tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt
    13621 ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa
    13681 caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat
    13741 ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca
    13801 atgttcattt ttataac
//
LOCUS       DACECB010000071        13702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECB010000071 DACECB010000000
VERSION     DACECB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.071"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..903)
                     /locus_tag="IWO04_002284"
     CDS             complement(<1..903)
                     /locus_tag="IWO04_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0213861.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="IWO04_002285"
     CDS             1186..2154
                     /locus_tag="IWO04_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0213862.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="IWO04_002286"
     CDS             2267..2545
                     /locus_tag="IWO04_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0213863.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2663..2899
                     /gene="secG"
                     /locus_tag="IWO04_002287"
     CDS             2663..2899
                     /gene="secG"
                     /locus_tag="IWO04_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0213864.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3011..3757
                     /locus_tag="IWO04_002288"
     CDS             3011..3757
                     /locus_tag="IWO04_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0213865.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3760..6117
                     /gene="rnr"
                     /locus_tag="IWO04_002289"
     CDS             3760..6117
                     /gene="rnr"
                     /locus_tag="IWO04_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0213866.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6139..6603
                     /gene="smpB"
                     /locus_tag="IWO04_002290"
     CDS             6139..6603
                     /gene="smpB"
                     /locus_tag="IWO04_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0213867.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6905..7624
                     /gene="atpB"
                     /locus_tag="IWO04_002291"
     CDS             6905..7624
                     /gene="atpB"
                     /locus_tag="IWO04_002291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0213868.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7677..7892
                     /gene="atpE"
                     /locus_tag="IWO04_002292"
     CDS             7677..7892
                     /gene="atpE"
                     /locus_tag="IWO04_002292"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0213869.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7983..8507
                     /gene="atpF"
                     /locus_tag="IWO04_002293"
     CDS             7983..8507
                     /gene="atpF"
                     /locus_tag="IWO04_002293"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0213870.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8494..9036
                     /locus_tag="IWO04_002294"
     CDS             8494..9036
                     /locus_tag="IWO04_002294"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0213871.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9059..10615
                     /locus_tag="IWO04_002295"
     CDS             9059..10615
                     /locus_tag="IWO04_002295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0213872.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10629..11531
                     /locus_tag="IWO04_002296"
     CDS             10629..11531
                     /locus_tag="IWO04_002296"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0213873.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11554..12960
                     /gene="atpD"
                     /locus_tag="IWO04_002297"
     CDS             11554..12960
                     /gene="atpD"
                     /locus_tag="IWO04_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0213874.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12974..13396
                     /locus_tag="IWO04_002298"
     CDS             12974..13396
                     /locus_tag="IWO04_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0213875.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4531 a         2451 c         2988 g         3732 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt
     2581 attccattta tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat
     2641 cttttgggaa ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc
     2701 gattgttatg gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc
     2761 atttaccggc ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt
     2821 catgcagcga tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc
     2881 atatttatct tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat
     2941 tgcaaagaga acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga
     3001 ggtgagacga gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt
     3061 tttgctgctt cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt
     3121 ggaaaaagca gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc
     3181 atatgatatc ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca
     3241 ttttttacag tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat
     3301 ctttgctgta cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc
     3361 tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct
     3421 gaagacagca ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt
     3481 agctgaacaa caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc
     3541 agatatccaa cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa
     3601 tggcgtattt gaaactgcac gcaagttgag ccgtcagcga gtgacacttc aatggtatcc
     3661 tgaaagtggt catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt
     3721 ggaattttta gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa
     3781 gagaaaattc tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc
     3841 gctgaaggtt tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct
     3901 caaatggaaa gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta
     3961 aaaccaatct tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg
     4021 attgatcccg aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat
     4081 ggagacaccg tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca
     4141 gaggggaaag tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt
     4201 gcttattcag aagaagaaat acaagaaaca gatctttacg gcgttgttat cccgaaagat
     4261 agaaagctca accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga
     4321 agtattgtgc tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa
     4381 gggataatca agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt
     4441 gtcgtggcta atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa
     4501 gttccagatc aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc
     4561 attgttacga ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa
     4621 ttagataacg gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca
     4681 caaggcagtc aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat
     4741 cgagtgattc cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat
     4801 gttccaaggc tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct
     4861 catgatattt tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat
     4921 caaatcttag aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg
     4981 ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg
     5041 atttcttttg aggatcgcga agcgaaaatc cttgttgatg ctgaaggaca cccaaatgac
     5101 atccagctaa gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc
     5161 aatgaaacgg tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat
     5221 gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt
     5281 ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga
     5341 gtagaagaca aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa
     5401 gcacgctatt cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac
     5461 tttacatctc ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac
     5521 agtgaagata aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca
     5581 gatcatagtt ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg
     5641 aagaaagcag aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct
     5701 gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc
     5761 aatgaattga aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa
     5821 agaactggtc tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat
     5881 ccagatgaac gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa
     5941 cgtccaaaag cacgcaatgg aaaatttgga aaacgagaag gaaaacaagc aggcaaacgc
     6001 tcagaaaaaa atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag
     6061 aaaaaaggga aaaagccatt ttacaaagat gtcaaaaaga agaaaaagaa aaaataaaat
     6121 gagttggagg aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa
     6181 gctcgccatg attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg
     6241 gagatcaagt cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc
     6301 aacggggaag ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt
     6361 aatcatgatc cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt
     6421 gaaaacgaat tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac
     6481 ggctatgcca aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa
     6541 gatttgaaac gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga
     6601 taatttcatt atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt
     6661 gttagcattt aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc
     6721 gtgcatgggg aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg
     6781 aaaatgtttt gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg
     6841 gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa
     6901 tggattggat gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt
     6961 ttgtataatg gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag
     7021 aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag
     7081 tcgaggaatc gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc
     7141 atttacattg tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt
     7201 cttacctaac gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac
     7261 tttggcattt ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa
     7321 aggatatttc gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat
     7381 tgaagaattt acaaatttat taacacttgc tttacgtctt tatgggaata tttatgcggg
     7441 ggaagttttg ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc
     7501 attagcaatt ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca
     7561 agcatttgtc tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga
     7621 ataatactcg gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga
     7681 attatatcgc agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg
     7741 gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat
     7801 tccgtacaac aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag
     7861 ttatcgcctt gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca
     7921 tcgtttgtta ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc
     7981 ctatgctgaa tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg
     8041 ttgtcagcgg ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa
     8101 tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac
     8161 aatctcgcat caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc
     8221 gttctgatgc agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa
     8281 atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata
     8341 tcactgtaga acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc
     8401 ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa
     8461 tcaatcaata cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga
     8521 agacgttacg ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc
     8581 tatcaagatc tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg
     8641 ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc
     8701 ggttatgatg ggatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat
     8761 gaccttttgt tcatgatcga agaatatgaa cgtcgatatg atgaacatca agggttacta
     8821 ttgggaagtg tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag
     8881 aatgttgcaa aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct
     8941 attattggtg gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg
     9001 caattagaaa aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat
     9061 ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa
     9121 cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc
     9181 acatggttta gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg
     9241 aatggcgcaa aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc
     9301 cattcgtgaa ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga
     9361 ggccttgatc ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat
     9421 cgttactgac aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc
     9481 tgttaacgaa ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg
     9541 cggacaacgt gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgatcga
     9601 tacgatcatc aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa
     9661 agattctaca gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac
     9721 aatcgttgtg aatgccggtg cgtctcaacc agcaccattg ctttatatcg caccatatgc
     9781 tggtactgca atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga
     9841 tgatctttca aaacaagctg ttgcttatcg tgaactttcc ttgctgcttc gccgtccacc
     9901 aggtcgtgaa gcttatccag gggatgtttt ctacttacac tcacgcttat tggaacgtgc
     9961 agcaaaatta agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac
    10021 acaagctgga gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca
    10081 aatcttctta gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact
    10141 atctgtatct cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac
    10201 tttacgtcta gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga
    10261 cttggatgca gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa
    10321 acaaaaattg catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac
    10381 acatggattc ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt
    10441 cgaatatctg gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct
    10501 tccagaagaa gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac
    10561 aaaaggaaat aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg
    10621 tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa
    10681 cgagccaaat cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag
    10741 cttcttcaca gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga
    10801 ctgctactca gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca
    10861 gcatgttgct cagccgtcct gttaagaaaa cgggttatat cgtcatcact gcagatggcg
    10921 gtttagtcgg tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag
    10981 accaccaatc acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct
    11041 ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct
    11101 ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg
    11161 atgaattata cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag
    11221 aaaaaatgct gccaatctca gacttagatc cagaagaagc aactactttc gaacaagaat
    11281 atatttttga accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa
    11341 gtttgatcta cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag
    11401 caatgaaaac agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttaca
    11461 accgtgcaag acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg
    11521 cactagaata gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta
    11581 atcggtcccg ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac
    11641 gcattagttg tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta
    11701 gaattagggg acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga
    11761 ggaatggaag tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta
    11821 ggtcgcgtat tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat
    11881 gcggaaagaa gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca
    11941 gaaattttgg aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg
    12001 aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc
    12061 cataatatcg cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact
    12121 cgtgaaggta atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc
    12181 atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt
    12241 ttgacgattg cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac
    12301 aacattttcc gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct
    12361 tctgcggttg gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc
    12421 acatctacga aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac
    12481 tacactgacc cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa
    12541 cgtaaattaa cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt
    12601 gctttagcac ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac
    12661 gtattgcaac gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta
    12721 tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa
    12781 aactttaacg tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa
    12841 acagtgaaag gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct
    12901 ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag
    12961 aaaggggtgc cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt
    13021 ctatgatcac catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt
    13081 gcctaaacat gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac
    13141 agattcagat acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga
    13201 taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg
    13261 tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga
    13321 tacgaacgaa ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt
    13381 atctaagcac tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga
    13441 ggttatgaca gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc
    13501 aaatttttgt gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc
    13561 ggaggttgtg aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc
    13621 tcatattttg ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt
    13681 ggaataaggg actgcgacaa ga
//
LOCUS       DACECB010000072        13344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECB010000072 DACECB010000000
VERSION     DACECB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.072"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1363)
                     /locus_tag="IWO04_002299"
     CDS             complement(44..1363)
                     /locus_tag="IWO04_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0213876.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1360..2013)
                     /locus_tag="IWO04_002300"
     CDS             complement(1360..2013)
                     /locus_tag="IWO04_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213877.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2301..2821
                     /locus_tag="IWO04_002301"
                     /pseudo
     CDS             2301..2821
                     /locus_tag="IWO04_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2723..3439)
                     /locus_tag="IWO04_002302"
     CDS             complement(2723..3439)
                     /locus_tag="IWO04_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0213878.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3563..3748)
                     /locus_tag="IWO04_002303"
     CDS             complement(3563..3748)
                     /locus_tag="IWO04_002303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213879.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3975..5248
                     /locus_tag="IWO04_002304"
                     /pseudo
     CDS             3975..5248
                     /locus_tag="IWO04_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5355..6386)
                     /locus_tag="IWO04_002305"
     CDS             complement(5355..6386)
                     /locus_tag="IWO04_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0213880.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6393..7223)
                     /locus_tag="IWO04_002306"
     CDS             complement(6393..7223)
                     /locus_tag="IWO04_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213881.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7220..8026)
                     /locus_tag="IWO04_002307"
     CDS             complement(7220..8026)
                     /locus_tag="IWO04_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0213882.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8032..8856)
                     /locus_tag="IWO04_002308"
     CDS             complement(8032..8856)
                     /locus_tag="IWO04_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0213883.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8846..9946)
                     /locus_tag="IWO04_002309"
     CDS             complement(8846..9946)
                     /locus_tag="IWO04_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0213884.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10124..10909
                     /locus_tag="IWO04_002310"
     CDS             10124..10909
                     /locus_tag="IWO04_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0213885.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10942..11325
                     /locus_tag="IWO04_002311"
     CDS             10942..11325
                     /locus_tag="IWO04_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0213886.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11401..11532
                     /locus_tag="IWO04_002312"
     CDS             11401..11532
                     /locus_tag="IWO04_002312"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0213887.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11501..11737
                     /locus_tag="IWO04_002313"
     CDS             11501..11737
                     /locus_tag="IWO04_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0213888.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11815..12786)
                     /locus_tag="IWO04_002314"
     CDS             complement(11815..12786)
                     /locus_tag="IWO04_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0213889.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4054 a         2912 c         2306 g         4072 t
ORIGIN      
        1 taagtctcta tttttcaata actattgcaa aattatatct taattaagaa aatattttta
       61 ttttaaagat aaaaaattct tcatcctgca atactttata ttctatattg taattattca
      121 gaatattact tacaatatat aaacctaatc cattactatt ttccttattt aaatcaaatt
      181 taacatcaaa tattttatcc atattcgaaa ttttattgtt accgtatgaa ttctctatat
      241 ataaccaatc attaactatc ccaatattaa ttaccccatt tacatcagta tattttaccg
      301 cattactaat caaattagat agaataatct ttaaagctgt ttttcctata taaacattct
      361 cgtctaatat ataattattg acagttattt tcttttgatt tgctaatatt tcatactttt
      421 ttaatatgtc ttctaatgta tcatttattt tcaaatattc ttcatcatta ttcaaatttt
      481 ctatagaatg aactgataat atttgagaaa tattttttgt taaactatca acgatttcaa
      541 tacattcatt aatataaata tctctttctt tatattttcc aatattgtat ttcatgtttt
      601 ccagtattat tttaagacta gcaagagggg ttttaagttc atgggatgca cctttaaaaa
      661 agtcatattt taacttttct aattttaaaa tttctttatt tttaaattcc aaatcgtcaa
      721 ttgttcttaa taaagtagaa tacaaatcat taatttgttg ttttaattct ccaacttcat
      781 cattagagct aacttttaaa ctcatttttt tatcaagttt catcatttta tcagtaacaa
      841 tttttatttc ttgtatatta ttttttattg attttgcata aattaaagaa ataatagcag
      901 aaaataaaat tgatattaat aaagaatatg gtaaaaattt aaggcttaaa tctttcgcat
      961 ctttttgcat atcagctgta gaaacaaatt gtagatttgt ttttttacca tcattaagct
     1021 ttatttctct ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat
     1081 tatctttgat ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac
     1141 tactcttgga ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat
     1201 tactagaaat ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt
     1261 taggaaagat aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa
     1321 atatttgtat gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata
     1381 tcctacattt cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat
     1441 atatacatct attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat
     1501 aatttgcatt cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag
     1561 ttcttttgca tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt
     1621 aaccgataga tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga
     1681 atctattcta gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa
     1741 attagtaaat gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg
     1801 taaattgctt ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg
     1861 aatgtctaag ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc
     1921 tttagcttga ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat
     1981 catattatca tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact
     2041 agatattata cagcatatta tggctctata atatctagtg ttggtgacgc cttctgttgc
     2101 caatactttt tctttttttt aataaatgca aacagacacc ttgaaatcct ttagaaaaaa
     2161 ggcttctata atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt
     2221 cttcgtttca actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac
     2281 cctaccaaag gagtcgctct atggataatc atactagaaa attactcgga ttaacagata
     2341 aacatttatt ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc
     2401 agtttattaa agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc
     2461 gtattatccg caacggttcc tatacgacac gtactcagat gttgaaagtc aaagaaaagt
     2521 tgactatttt agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt
     2581 ctgcgaaaac ggacttagtt gatgaacatc atcaactaac aaaggaactg aagcaggcga
     2641 ttctcataga actgtacgaa aatcaatcac gtaatctgaa aatcagaaga agtaccgtcg
     2701 gctcaagcga tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg
     2761 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt
     2821 gtgcacaatg gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat
     2881 cacggggatg gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt
     2941 gcggtccgta aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca
     3001 gttcttgctc ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc
     3061 ctgcaatgtt cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt
     3121 aaagattgac aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca
     3181 attgacgttt gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa
     3241 aggcgaaaaa atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg
     3301 caccgactgg actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca
     3361 acaaataacc ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat
     3421 caattttttc cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag
     3481 aattgagact actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc
     3541 actggtctcc caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca
     3601 acaccttgtt gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct
     3661 tcagtaaata gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat
     3721 caaaatcaaa aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag
     3781 ctgtgtcctg ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg
     3841 aaagaaatgt ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg
     3901 agcataaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     3961 aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg
     4021 gaaacattca aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg
     4081 tttctgccac gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac
     4141 atgggctgat cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga
     4201 atcaacggac ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct
     4261 attttacggc gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt
     4321 ataaaatatt ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg
     4381 gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga
     4441 aaataaattg gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag
     4501 tcggtcccta ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc
     4561 cttcacgtaa gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt
     4621 taaacgtaaa aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt
     4681 gctttcctca tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca
     4741 gttttaagga tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag
     4801 ccaaacacta tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc
     4861 ttgtttatga taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg
     4921 aagtcgtaga acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc
     4981 aagaaattct tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa
     5041 caatgcccaa gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg
     5101 aatccggcat tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc
     5161 atacccacat caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt
     5221 cttaatcaat catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat
     5281 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt
     5341 ttgccgacta tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt
     5401 gctagaatat ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc
     5461 aaccgatcca ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg
     5521 cctaaaataa atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca
     5581 aattgaatca gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg
     5641 tgggtatact gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca
     5701 acaaatcggc tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc
     5761 ccagcttcca tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc
     5821 acaaaaatca tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt
     5881 tttctaattc gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca
     5941 gtgaccaatg agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca
     6001 tttttatctg cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga
     6061 atcacctcag gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc
     6121 atagttggaa acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc
     6181 ttatcggcaa tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc
     6241 gtgatacagc gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc
     6301 tcaccctttt caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga
     6361 ttgagatcat gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc
     6421 gactgcttct cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc
     6481 ataaaggtag ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta
     6541 gccaaatggc cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga
     6601 ccgccaatca acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga
     6661 gaaactaggg tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac
     6721 atcggggcgc ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc
     6781 tggttgttaa gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac
     6841 actacaaaca ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc
     6901 actcctggta aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg
     6961 aaatacaaat gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc
     7021 acacctgcta ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc
     7081 tgttggaacc attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag
     7141 ctggcataca aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa
     7201 atcggtttta ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca
     7261 ttgccgttac cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg
     7321 cgaacacatc tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc
     7381 tggtcagggc atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg
     7441 tgctggcaat cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg
     7501 aagcgcccag caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat
     7561 acaaaaacaa gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc
     7621 aagaatacaa attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc
     7681 ccaacaaaat aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg
     7741 aaacaaaggt gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa
     7801 taaacgtccc gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa
     7861 aggcctgtgt gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt
     7921 tagttaaatc actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg
     7981 cacagaagat aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct
     8041 catcaattct tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa
     8101 caatggagcc gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga
     8161 aatcaaataa aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc
     8221 atccatcccg tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag
     8281 gtaaatagcc aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc
     8341 ggtaaatttg tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg
     8401 atactgattc actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc
     8461 actataaaag atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa
     8521 acgattatag ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt
     8581 ttttccgcca gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg
     8641 gtaattacta tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa
     8701 cggacgtgaa ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc
     8761 aactaaatga ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag
     8821 tataactaca atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa
     8881 gctctggaag ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg
     8941 cttcataagc atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc
     9001 gaataggtcg cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt
     9061 cccgtgctag ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc
     9121 cgctaatgat ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt
     9181 cacgataatt tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa
     9241 gatttgctac gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt
     9301 aattcagacc tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata
     9361 cagcaaattg tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct
     9421 cttgcagctc cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg
     9481 aaatggttcc cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa
     9541 tttcctccca atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac
     9601 cgaagctgat accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact
     9661 tagccaattc ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt
     9721 caaggtcgtt ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa
     9781 tctctccttc ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg
     9841 attgcttcgt tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac
     9901 aaaataaacc gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc
     9961 tttcgataat tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt
    10021 tcatgaacaa tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt
    10081 ctaaagcttt tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca
    10141 gcatctccac acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta
    10201 tcagccggag tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa
    10261 agatgacatt ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta
    10321 ttccaccaaa tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat
    10381 cgtggattac ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg
    10441 ccgctttgat tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga
    10501 atattttgat cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca
    10561 ctttgattat ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga
    10621 ggctcagctg ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa
    10681 tgccaagagc ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta
    10741 tcaaagtgtc ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta
    10801 tcagctgggc tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct
    10861 agtcattcaa ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa
    10921 tagaagaaag tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc
    10981 cattatttaa ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg
    11041 ttagcagccg ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg
    11101 gaaaaagttc aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg
    11161 cagtttcatc ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag
    11221 ctgcaattga cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc
    11281 cttggtgatc aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc
    11341 gtttcagttg caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt
    11401 gtgctggtca ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg
    11461 gctgtagcca actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc
    11521 tcgtgtcatt aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc
    11581 tgtttccagc tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa
    11641 tggcgccttg gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt
    11701 gcaaaacaca aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga
    11761 ggactaccgg agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa
    11821 atcgtagcag cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg
    11881 ttcattcgac aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa
    11941 aagctctatt cttgagctgg ctgcagctag tgagtgtctt tcatttatcc ataagataaa
    12001 gcttggattc aataaataaa taaatgtttt ggttaaagct gtgaccagca aatgaggaac
    12061 cgtgcgagcc agttgatctg ccaatgcaaa catttcttta ggtgaaggcg ctggcgtgtc
    12121 tgccaacgga tactccagtc ccaatgcttg gtaaccgtgc ataccatagc agtggtacgg
    12181 tagtaattct aacggtacga accgatcaag atctgccaca aaacaagcaa tcgtcagcag
    12241 ctcggtcagg ttcatattga tacctggaac cacaggaact cgaattcgca cttcgcagtt
    12301 aggatactca attaaaaagc gacgaatatt cttcaaaatt tgttggttat ctaagccggt
    12361 ccattgtttg tggagcttgc tggtaaacat ttttacatcc acaaacaatg tatccacata
    12421 gggagctacc ttttggattt cattataagc accacaaaag ctggtttcca gtacggtatt
    12481 gattccaatg tatttcgatt tttgcaaaat ttctttagca aaatccgcct gaaccagtgc
    12541 ctcgcctccg ctaatggtca ctccgccatc cgagtgaaag taaaaaacag catccttctc
    12601 aatctcatcc attacctctt cagcggtcat ggtctggcca aatccttttt caatctgctt
    12661 gctttgtgat tcgggaatcg aacaccaggc acagcgtaat gggcagccct ttaaataaac
    12721 aacggtccgt aaaccagcac catcatgtag agaaccctgt tctattctta atactgttcc
    12781 ttccatatag atttttgaat tacacactag tttaaactaa aacacacaag gaaaaactta
    12841 ccatttaatc ataaaattag ctcatataaa tatactagga tgtaaaatag actggtcaaa
    12901 atctggtcaa aaaagaggac taatacaggt agtcctcttt tttgttattt taatattcta
    12961 aatcattttt tattggttta ttcccttatt cgatgtagac tcaaatcact atatcactag
    13021 atatcaaacg gcgtcatacg gaatcaaaaa catcaaattt gacctgttaa atggtcattg
    13081 aataacattc aaaatcaata aaacggagtc agacacggag tcaaaaacgc tgagagtaaa
    13141 aaatatatac agccttgtag gtaaagtacc acagggctgt atatttttta tttcagatta
    13201 ccttaaatga ttccatttac tttttatctc attttttcct actagttagt atcaaatact
    13261 ctcctatttt ctcaataaac tatttttgga tacatggtat ctgaaacagc taaaaccttg
    13321 tcattacagc aaaaaatcac gtta
//
LOCUS       DACECB010000073        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECB010000073 DACECB010000000
VERSION     DACECB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.073"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..150)
                     /locus_tag="IWO04_002315"
     tRNA            complement(78..150)
                     /locus_tag="IWO04_002315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IWO04_002316"
     CDS             531..1100
                     /locus_tag="IWO04_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0213890.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IWO04_002317"
     CDS             1087..1947
                     /locus_tag="IWO04_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0213891.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IWO04_002318"
     CDS             2137..2841
                     /locus_tag="IWO04_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213892.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IWO04_002319"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IWO04_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0213893.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IWO04_002320"
     CDS             4678..5994
                     /locus_tag="IWO04_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213894.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IWO04_002321"
     CDS             5995..6864
                     /locus_tag="IWO04_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213895.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IWO04_002322"
     CDS             6919..7728
                     /locus_tag="IWO04_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0213896.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IWO04_002323"
     CDS             complement(7831..8460)
                     /locus_tag="IWO04_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0213897.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IWO04_002324"
     CDS             8630..9922
                     /locus_tag="IWO04_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213898.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IWO04_002325"
     CDS             complement(9998..10660)
                     /locus_tag="IWO04_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0213899.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IWO04_002326"
     CDS             10885..11169
                     /locus_tag="IWO04_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0213900.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IWO04_002327"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IWO04_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0213901.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccattc
//
LOCUS       DACECB010000074        13085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECB010000074 DACECB010000000
VERSION     DACECB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.074"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..2199)
                     /locus_tag="IWO04_002328"
     CDS             complement(79..2199)
                     /locus_tag="IWO04_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0213902.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IWO04_002329"
     CDS             2429..3607
                     /locus_tag="IWO04_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0213903.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IWO04_002330"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IWO04_002330"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0213904.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IWO04_002331"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IWO04_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0213905.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IWO04_002332"
     CDS             complement(4960..6213)
                     /locus_tag="IWO04_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0213906.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IWO04_002333"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IWO04_002333"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0213907.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IWO04_002334"
     CDS             7790..9136
                     /locus_tag="IWO04_002334"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0213908.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="IWO04_002335"
     CDS             complement(9444..9830)
                     /locus_tag="IWO04_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0213909.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10709..10921
                     /locus_tag="IWO04_002336"
     CDS             10709..10921
                     /locus_tag="IWO04_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213910.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="IWO04_002337"
     CDS             complement(11199..12998)
                     /locus_tag="IWO04_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0213911.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DACECB010000075        12925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECB010000075 DACECB010000000
VERSION     DACECB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.075"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..628)
                     /locus_tag="IWO04_002338"
     CDS             complement(5..628)
                     /locus_tag="IWO04_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213912.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IWO04_002339"
     CDS             complement(787..2184)
                     /locus_tag="IWO04_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0213913.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IWO04_002340"
     CDS             complement(2216..3187)
                     /locus_tag="IWO04_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0213914.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IWO04_002341"
     CDS             3561..3986
                     /locus_tag="IWO04_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213915.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IWO04_002342"
     CDS             complement(4474..5175)
                     /locus_tag="IWO04_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0213916.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="IWO04_002343"
     CDS             complement(5191..5631)
                     /locus_tag="IWO04_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0213917.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IWO04_002344"
     CDS             complement(5631..6206)
                     /locus_tag="IWO04_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0213918.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7054..7533)
                     /locus_tag="IWO04_002345"
                     /pseudo
     CDS             complement(7054..7533)
                     /locus_tag="IWO04_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7692..8393)
                     /locus_tag="IWO04_002346"
     CDS             complement(7692..8393)
                     /locus_tag="IWO04_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0213919.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8501..9910)
                     /locus_tag="IWO04_002347"
     CDS             complement(8501..9910)
                     /locus_tag="IWO04_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0213920.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9958..11823)
                     /locus_tag="IWO04_002348"
     CDS             complement(9958..11823)
                     /locus_tag="IWO04_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0213921.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11944..12795)
                     /locus_tag="IWO04_002349"
     CDS             complement(11944..12795)
                     /locus_tag="IWO04_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0213922.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3689 a         2786 c         2109 g         4341 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
     2041 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
     2281 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
     2341 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
     2401 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
     2461 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
     2521 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
     3421 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     6361 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     6421 gggatttcgt cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt
     6481 ttattaacga aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc
     6541 taaataaaaa agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc
     6601 tcctaaaaat ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg
     6661 tcactgctgg aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt
     6721 ttgatattgg gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact
     6781 gtataataac tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct
     6841 tcgacataag gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt
     6901 catttcaagg agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt
     6961 atattcgatt ctttctaact ctgattcttt cataccattc ctgcctcaag gtacatctat
     7021 tgtacttttt tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga
     7081 tatgtcttct tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt
     7141 caataataaa atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc
     7201 ccatggtctg gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc
     7261 gccccaaaac atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt
     7321 gcgtgccaag tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat
     7381 aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat
     7441 tttccctaag aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa
     7501 tacagtgatt ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata
     7561 aatggtaatc cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat
     7621 gagaagctat ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     7681 tcttttttta tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc
     7741 gcctccgaat attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg
     7801 ccgatctgtg cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag
     7861 aaatcatcag aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat
     7921 tctttgccaa attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa
     7981 taaatataag gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg
     8041 aaaaaggaaa acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct
     8101 ttccggactt catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat
     8161 cgattgtaag gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat
     8221 tcatctgatg ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt
     8281 tcttctaatt cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata
     8341 tcttctttaa ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca
     8401 ccctttgctt tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc
     8461 acatgtgaga agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg
     8521 aggcaattac ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat
     8581 ttccttcatc gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg
     8641 atgcactgac aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg
     8701 ctactgcatt agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa
     8761 taatcgtgcc atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata
     8821 atggtttctg gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga
     8881 tctgccagcc ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc
     8941 cttctgaagt tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa
     9001 atccgatgta gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta
     9061 atttttccgg atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc
     9121 cacgtacttg gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg
     9181 cctgattgac tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga
     9241 tttggatatc cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt
     9301 gattatgcca tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc
     9361 cttgacttaa agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt
     9421 tgactttact gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca
     9481 gttttcgatt tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag
     9541 aaatcgtgat cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa
     9601 attctaatcc cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg
     9661 caatcgaaaa acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga
     9721 aacgatcgta atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg
     9781 tccagtcaaa ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat
     9841 ctgcaacgga tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca
     9901 gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta
     9961 ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat
    10021 atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc
    10081 tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg
    10141 tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat
    10201 caaaatctcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt
    10261 cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc
    10321 ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt
    10381 acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc
    10441 atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc
    10501 tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca catttgactc
    10561 tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga
    10621 gaaagctttt actccggaaa atccgataat agctccacca atacctccag aaatacaagc
    10681 ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc
    10741 aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg
    10801 caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat
    10861 caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg
    10921 catgccgaac atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg
    10981 actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat
    11041 tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa
    11101 aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac
    11161 ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac
    11221 tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata
    11281 cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc
    11341 tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat
    11401 ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc
    11461 tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat
    11521 atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag
    11581 accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg
    11641 atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt
    11701 atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact
    11761 tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc
    11821 cattgttttt tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca
    11881 gtcacaaaca ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa
    11941 tccttatgat tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata
    12001 aatctgctct gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg
    12061 ttcgacacaa tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc
    12121 ttgctcgtca gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat
    12181 gatacgctga taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat
    12241 actcagtata tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc
    12301 ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac
    12361 acccgtcttt tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata
    12421 aaattttttt gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt
    12481 gatcgcataa tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt
    12541 tagtttatct tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat
    12601 atcagccatc agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt
    12661 ttggaccgct tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc
    12721 catacaaacg acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat
    12781 tttatcaatt ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc
    12841 tactgtttct tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt
    12901 caaaatcatc ataccatcga atttt
//
LOCUS       DACECB010000076        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECB010000076 DACECB010000000
VERSION     DACECB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.076"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO04_002350"
     CDS             6..305
                     /locus_tag="IWO04_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213923.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO04_002351"
     CDS             378..1739
                     /locus_tag="IWO04_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0213924.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO04_002352"
     CDS             1761..2021
                     /locus_tag="IWO04_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213925.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO04_002353"
     CDS             2035..2787
                     /locus_tag="IWO04_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0213926.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO04_002354"
     CDS             complement(2881..3486)
                     /locus_tag="IWO04_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0213927.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO04_002355"
     CDS             complement(3539..4513)
                     /locus_tag="IWO04_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0213928.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO04_002356"
     CDS             complement(4891..5670)
                     /locus_tag="IWO04_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0213929.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO04_002357"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO04_002357"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0213930.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO04_002358"
     CDS             7161..8558
                     /locus_tag="IWO04_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0213931.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO04_002359"
     CDS             8639..9079
                     /locus_tag="IWO04_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0213932.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO04_002360"
     CDS             9227..9553
                     /locus_tag="IWO04_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0213933.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO04_002361"
     CDS             9554..10303
                     /locus_tag="IWO04_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213934.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO04_002362"
     CDS             complement(10383..10838)
                     /locus_tag="IWO04_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0213935.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO04_002363"
     CDS             complement(11265..11540)
                     /locus_tag="IWO04_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213936.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO04_002364"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO04_002364"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0213937.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2174 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt cattccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACECB010000077        12636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.077, whole genome shotgun sequence.
ACCESSION   DACECB010000077 DACECB010000000
VERSION     DACECB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.077"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(146..1111)
                     /locus_tag="IWO04_002365"
     CDS             complement(146..1111)
                     /locus_tag="IWO04_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0213938.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="IWO04_002366"
     CDS             1345..2190
                     /locus_tag="IWO04_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0213939.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="IWO04_002367"
     CDS             2381..3568
                     /locus_tag="IWO04_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0213940.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="IWO04_002368"
     CDS             3574..3930
                     /locus_tag="IWO04_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0213941.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="IWO04_002369"
     CDS             3932..4270
                     /locus_tag="IWO04_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0213942.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="IWO04_002370"
     CDS             4665..5699
                     /locus_tag="IWO04_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0213943.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="IWO04_002371"
     CDS             5692..6378
                     /locus_tag="IWO04_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0213944.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="IWO04_002372"
     CDS             6402..7250
                     /locus_tag="IWO04_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213945.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="IWO04_002373"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="IWO04_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0213946.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="IWO04_002374"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="IWO04_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0213947.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="IWO04_002375"
     CDS             9516..10751
                     /locus_tag="IWO04_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0213948.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="IWO04_002376"
     CDS             10738..11208
                     /locus_tag="IWO04_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0213949.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="IWO04_002377"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="IWO04_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0213950.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2367 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatat
//
LOCUS       DACECB010000078        12282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECB010000078 DACECB010000000
VERSION     DACECB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.078"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1918)
                     /locus_tag="IWO04_002378"
     CDS             complement(<1..1918)
                     /locus_tag="IWO04_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ0213951.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLT"
     gene            2064..2639
                     /locus_tag="IWO04_002379"
     CDS             2064..2639
                     /locus_tag="IWO04_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0213952.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            complement(2990..4303)
                     /locus_tag="IWO04_002380"
     CDS             complement(2990..4303)
                     /locus_tag="IWO04_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAQ0213953.1"
                     /translation="MATLHENRLLFNSNVTVSHSGGNLSSDSGLILAKEFMNKFEFSQ
                     ILCKNIQIQDDRLYHVHENESILEQIILQLIAGYPTDSSANILATDPIFQAVLSKKRL
                     ASQSSISRFWDRISTENIVQLQKVNQILIDKARTIRNTNEMIFDLDSTHSDTFGNQEM
                     TDYNAHYQTTGYHPLVAFDGLTGDFLKAELRSGNVYTSKGVAAFTRPLFEHYHSVTPV
                     STIMVRADSGFAMPDLYELCEEYDSLYTIRLKSNRNLYRIADQFVTIKDHHDWSKKEV
                     HYYNATYQAKSWGKSRRICIKSTREASELLFRHEFIITNFSKDVSPEMVFQTYSKRGT
                     MENYIKEAKNGFYLDKTDSPRFIENHARMIVSLLAYNIVNFMRTLCFTNETKGYQVST
                     IRLFLFKIAGKIVHSGRRQYLKLSSYHVYQKLFYRILQNIQVLQC"
     gene            4533..5228
                     /gene="vanR"
                     /locus_tag="IWO04_002381"
     CDS             4533..5228
                     /gene="vanR"
                     /locus_tag="IWO04_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0213954.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5206..6360
                     /locus_tag="IWO04_002382"
     CDS             5206..6360
                     /locus_tag="IWO04_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0213955.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            6575..7543
                     /locus_tag="IWO04_002383"
     CDS             6575..7543
                     /locus_tag="IWO04_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0213956.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7536..8567
                     /gene="vanA"
                     /locus_tag="IWO04_002384"
     CDS             7536..8567
                     /gene="vanA"
                     /locus_tag="IWO04_002384"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0213957.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            8573..9181
                     /locus_tag="IWO04_002385"
     CDS             8573..9181
                     /locus_tag="IWO04_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0213958.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            9587..10525
                     /gene="vanY"
                     /locus_tag="IWO04_002386"
     CDS             9587..10525
                     /gene="vanY"
                     /locus_tag="IWO04_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0213959.1"
                     /translation="MVKEETENEEVVFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQN
                     YDQNPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHD
                     ELINGYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMG
                     AEYALPAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTG
                     IQYEPWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKN
                     TTIHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            10678..11163
                     /gene="vanZ"
                     /locus_tag="IWO04_002387"
     CDS             10678..11163
                     /gene="vanZ"
                     /locus_tag="IWO04_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0213960.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            11889..12149
                     /locus_tag="IWO04_002388"
     CDS             11889..12149
                     /locus_tag="IWO04_002388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213961.1"
                     /translation="MITKIEDITPLEITLQTENYHQVLSEYFGEAYWTSEFEVEEIDI
                     EENKKIFAVYNNELDEICQLGIITLTYENKKIKSIHLLREIL"
BASE COUNT         3958 a         2173 c         2485 g         3666 t
ORIGIN      
        1 atgtaagtcc gggtgtggct tcggccatct tgctcaagcc aatattcatt cccattccta
       61 aaagggcagc catgataatg attgtttctt ccttatctgg ttttcgatta ttggaagcat
      121 gagtgaattg ctcatgaaat cctgttatat gggccacatc catgagtaaa tcagttaatt
      181 ttattcttgg tagcatctga taaaggcttg cactaaattt ttttgcttct tctggaacat
      241 ctttttctaa gcgtgcaagt gatagctttc ctttttcaag agaaacccca tctaacttat
      301 tggaattggc agctaaccac tttaaccttt cattaaagct gctggttctc tccgttatat
      361 aatcttcgaa tgataaacta actgataatc tcgtattccc cttcgattga ttccatgtat
      421 cttccgaaaa caaatattcc tcaaaatccc tatattgtct gctgccaaca atggaaacat
      481 ctcctgcccg aacatgctcc cgaagttctg ttaaaacagc catttcatag taatgacgat
      541 taattgttgt accatcatcc tcgtataaat gtcttttcca tcgttttgaa ataaaatcca
      601 caggtgagtc atcaggcact tttcgctttc cagattcgtt cattcctcgg ataatctcaa
      661 cagcttgtaa aagtggctca tttgcctttg tagaatgaaa ttccaatact cttaatagcg
      721 ttggcgtata ttttcttagt gaataaaacc gtttttgcag taagtctaaa taatcatagt
      781 cggcaggacg tgcaagttcc tgagcctctt ctactgaaga gacaaaggta ttccattcaa
      841 taaccgattc taaaacctta aaaacgtcta atttttcctc tcttgcttta attaatgctt
      901 gtccgatgtt cgtaaagtgt ataactttct catttagctt tttaccgttt tgtttctgga
      961 tttcctcttg agccttacga ccttttgata acaaactaag tatttgccta tcatgaattt
     1021 caaacgcttt atccgttagc tcctgagtaa gttgtaataa atagatggtt aatatcgaat
     1081 aacgtttatt ttcttgaaag tcacggaatg catacggctc gtatcttgag cctaagcgag
     1141 acagctgcaa caggcggtta cggtgcaaat gactaatttg cactgtttct aaatccattc
     1201 ctcgtatgta ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa ggatgacccg
     1261 gtggctcttt taaccaaccc aatatcgttt tattggattc ggatggatgc tgcgaggtaa
     1321 taatcccttc aagcttttct ttttgctcat ttgttagaga tttactaacc gtattaaata
     1381 gcttcttttc agccattgcc cttgcttccc acaccattct ttcaagtgta gtgatagcag
     1441 gcagtataat tttgtttttt cttagaaaat ctatgcattc atgcagtaga tgaatggcat
     1501 caccattttc caaagctaat tgatgaaggt acttaaatgt cattcgatat tcactcaggg
     1561 taaaagttac aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa agtgtatttt
     1621 ccctttgagg ataatgatca agcgaggatg gactaacacc aatctgtttc gatatatatt
     1681 gtatgaccga atctgggatg cttttgatat gagtgtatgg ccaaccggga taccgaagaa
     1741 cagctaattg aacagcaaat cctaaacggt tttcttccct ccttcgctta ttaactattt
     1801 ctaaatcccg tttggaaaaa gtgaagtagg tccccagtat ccattcatct tcagggattt
     1861 gcataaaagc ctgtctctgt tccggtgtaa gcaattctct acctctcgca attttcattc
     1921 agtatcattc catttctgta ttttcaattt attagttcaa ttatatatca atagagtgta
     1981 ctctattgat acaaatgtag tagactgata aaatcatagt taagagcgtc tcataagact
     2041 tgtctcaaaa atgaggtgat attttgcgga aaatcggtta tattcgtgtc agttcgacta
     2101 accagaatcc ttcaagacaa tttcagcagt tgaacgagat cggaatggat attatatatg
     2161 aagagaaagt ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt
     2221 tacaggaaga tgacatcatt tatgttacag acttaactcg aatcactcgt agtacacaag
     2281 atctatttga attaatcgat aacatacgag ataaaaaggc aagtttaaaa tcactaaaag
     2341 atacatggct tgatttatca gaagataatc catacagcca attcttaatt actgtaatgg
     2401 ctggtgttaa ccaattagag cgagatctta ttcggatgag acaacgtgaa gggattgaat
     2461 tggctaagaa agaaggaaag tttaaaggtc gattaaagaa gtatcataaa aatcacgcag
     2521 gaatgaatta tgcggtaaag ctatataaag aaggaaatat gactgtaaat caaatttgtg
     2581 aaattactaa tgtatctagg gcttcattat acaggaaatt atcagaagtg aataattagc
     2641 cattctgtat tccgctaatg ggcaatattt ttaaagaaga aaaggaaact ataaaatatt
     2701 aacagcctcc tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc atcttaagaa
     2761 attcttagtc atttattatg tacctgaata attcatactt tttataaact accaaatttt
     2821 ataattatct gtttagtttt atgctcaatg gttctttttc tcaaaaatat ttttctaaag
     2881 ggtttaattt atttttgcta actgattaag tttaagaaag acctcatttt gggcgcactc
     2941 atcctatcgg atggttaacg aatttttttg aatggcgcaa taaacgcaac taacactgca
     3001 gtacctgaat gttttgtaga atccgataaa atagcttctg gtaaacatgg taactactta
     3061 atttcaagta ttgtcttctt ccggaatgaa ctatctttcc agcaatttta aagagaaata
     3121 agcgaatagt agaaacctga taccctttcg tttcattggt aaaacacagt gtccgcatga
     3181 agttcacgat attgtaagcc aataggctta cgatcatacg agcatgattt tctataaaac
     3241 gtgggctatc tgttttatcc aagtaaaagc cgttttttgc ttccttaatg taattttcca
     3301 tcgttcctct ttttgaatac gtttggaaaa ccatttctgg agagacgtct tttgaaaaat
     3361 tagtaatgat gaattcatgt ctaaaaagca attcactagc ttctctggtt gattttatgc
     3421 agatccttct ggattttccc catgactttg cttgataagt ggcgttatag taatgaactt
     3481 ctttcttact ccaatcgtga tgatccttaa tggtaacgaa ttgatcagcg attctgtaca
     3541 gattacgatt ggatttcaaa cggatagtat acaaactatc gtactcttca caaagctcat
     3601 acaaatcagg cattgcgaat ccactatctg cacgtaccat aatagtgctt actggtgtca
     3661 cagagtgata atgctcaaat aatggacgag taaatgctgc gacgcctttt gaggtgtaga
     3721 catttccaga acgaagttct gctttaagaa agtctccggt aagaccatca aaggcaacga
     3781 gtggatgata accggttgtc tggtaatgcg cattataatc ggtcatttct tggtttccaa
     3841 aggtatcact gtgagtagaa tctaaatcga aaatcatttc gttcgtattt cgaatcgtcc
     3901 gtgctttatc aatcaaaatc tggttaacct tttgtagctg gacgatattt tctgttgata
     3961 tccgatccca aaagcgagaa atggatgact gtgaagccaa ccgctttttg cttaaaacag
     4021 cttgaaagat aggatccgtt gctaatatat tggctgaaga atctgtagga tagcctgcaa
     4081 tcaactgcaa gatgatttgt tccagaatag attcattttc atgaacatga tacagtcggt
     4141 catcttgaat ctggatgttt ttacataaga tttggctaaa ctcaaatttg ttcatgaatt
     4201 cttttgctaa tattaagccg gaatctgagg acaaatttcc accagagtga gatactgtaa
     4261 catttgaatt gaaaagtaga cgattttcgt gtaaagttgc cattgagaga acccctttct
     4321 ttggttaggt ttagtcactt taaccatagc agattggggt tctttttgta cacccaaagg
     4381 gtgcgaataa acgaaacaaa atagacagtt ataacaaggt ttttgaaacg tttttgaaaa
     4441 actatgaatt attcaggatg taaatgctta taaattcggc cctataatct gataaattat
     4501 taagggcaaa cttatgtgaa agggtgataa ctatgagcga taaaatactt attgtggatg
     4561 atgaacatga aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt
     4621 tcaaatacta taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg
     4681 ccatattgga catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg
     4741 acaagcacac ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta
     4801 cagggttaac aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa
     4861 ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga
     4921 acgaaaatgt tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc
     4981 tgaacgagaa gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg
     5041 aaaacaaggg gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat
     5101 atttcagcaa gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga
     5161 acgacaccat tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg
     5221 aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca
     5281 attgttgtgg tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt
     5341 ggggattgga tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg
     5401 aaattatatc aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc
     5461 attagtattc ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag
     5521 ataaataccg gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg
     5581 gaaatggatg ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag
     5641 caggatgcaa agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat
     5701 attaaaacgc cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac
     5761 atgccggtag atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc
     5821 gaacagctaa tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta
     5881 acaaaaacgc acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct
     5941 cagctttccg cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc
     6001 ggcgaccctg ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac
     6061 agtgaggata acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc
     6121 gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc
     6181 tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt
     6241 gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat
     6301 acgacgttta gggtagagct tccagcgatg ccagacttgg ttgataaaag gaggtcctaa
     6361 gagatgtata taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt
     6421 aacaatttaa tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg
     6481 agccgttttc gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat
     6541 ttggtgcctt tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta
     6601 tggatgtgag caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat
     6661 ggcaacgata attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca
     6721 atgtatcagt gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag
     6781 agccggtgtg aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc
     6841 tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga
     6901 ttatactatg atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt
     6961 ggaaaaacat gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt
     7021 tggtgtggtg ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg
     7081 atgtaaagtg ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga
     7141 tgagttgctg caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca
     7201 ctatattatc agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac
     7261 tgggcgcggt ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact
     7321 gggcggtgcc gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac
     7381 ccaaaaacca attgataatc aatttttact taaacttcaa agaatgccta acgtgataat
     7441 cacaccgcat acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat
     7501 taaaaactgt ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac
     7561 tgtttggggg ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg
     7621 ctaacattaa taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat
     7681 ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac
     7741 tctcgccgga taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca
     7801 accatgttga tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac
     7861 aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa
     7921 tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg
     7981 ccttttgggt tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt
     8041 ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg
     8101 aattggacta cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg
     8161 ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagttgttg
     8221 gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc
     8281 cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc
     8341 gaggacggat acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag
     8401 cccgtgtgga tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc
     8461 tgcccggttt cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac
     8521 ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat
     8581 aggatttact tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac
     8641 ttgggataat ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac
     8701 atacgagttg gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg
     8761 attgcttcta tgggacggtt accgtcctaa gcgtgctgta aactgtttta tgcaatgggc
     8821 tgcacagccg gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga
     8881 gatgatttca aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga
     8941 tcttacgctt tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt
     9001 tatggatgaa cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg
     9061 cagacgtttg cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg
     9121 gcactatgta ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata
     9181 aacttttaac cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac
     9241 gtatgtaact ggttcttagg gaatttatat atagtagata gtattgaaga tgtaaggcag
     9301 agcgatattg cggtcattat ctgcgtgcgc tgcggcaaga tagcctgata ataagactga
     9361 tcgcatagag gggtggtatt tcacaccgcc cattgtcaac aggcagttca gcctcgttaa
     9421 attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag taaatccagt
     9481 agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa acgagattgt
     9541 gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag ttaaggagga
     9601 gactgaaaat gaagaagttg tttttttatt gttattgtta ttgttattct taatatactt
     9661 aggttatgac tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta
     9721 tgatcaaaat cccaaagaac atttagaaaa tagtgggact tctgaaaata cccaagagaa
     9781 aacaattaca gaagaacagg tttatcaagg aaatctgcta ttaatcaata gtaaatatcc
     9841 tgttcgccaa gaaagtgtga agtcagatat cgtgaattta tctaaacatg acgaattaat
     9901 aaatggatac gggttgcttg atagtaatat ttatatgtca aaagaaatag cacaaaaatt
     9961 ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt cattttatta ttaatagtgg
    10021 ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa atgggggctg agtatgcctt
    10081 accagcaggt tatagtgagc ataattcagg tttatcacta gatgtaggat caagcttgac
    10141 gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa aatgcttgga aatacgggtt
    10201 cattttacgt tatccagagg acaaaacaga gttaacagga attcaatatg aaccatggca
    10261 tattcgctat gttggtttac cacatagtgc gattatgaaa gaaaagaatt tcgttctcga
    10321 ggaatatatg gattacctaa aagaagaaaa aaccatttct gttagtgtaa atggggaaaa
    10381 atatgagatc ttttattatc ctgttactaa aaataccacc attcatgtgc cgactaatct
    10441 tcgttatgag atatcaggaa acaatataga cggtgtaatt gtgacagtgt ttcccggatc
    10501 aacacatact aattcaagga ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca
    10561 gcattattgt actagcactt ttggggtaac gttagctttt taatttaaaa cccacgttaa
    10621 ctaggacatt gctatactaa tgatacaact taaacaaaag aattagagga aattatattg
    10681 ggaaaaatat tatctagagg attgctagct ttatatttag tgacactaat ctggttagtg
    10741 ttattcaaat tacaatacaa tattttatca gtatttaatt atcatcaaag aagtcttaac
    10801 ttgactccat ttactgctac tgggaatttc agagagatga tagataatgt tataatcttt
    10861 attccatttg gcttgctttt gaatgtcaat tttaaagaaa tcggattttt acctaagttt
    10921 gcttttgtac tggttttaag tcttactttt gaaataattc aatttatctt cgctattgga
    10981 gcgacagaca taacagatgt aattacaaat actgttggag gctttcttgg actgaaatta
    11041 tatggtttaa gcaataagca tatgaatcaa aaaaaattag acagagttat tatttttgta
    11101 ggtatacttt tgctcgtatt attgctcgtt taccgtaccc atttaagaat aaattacgtg
    11161 taagatgtct aaatcaagca atctgatctt tcatacacat aaagatattg aatgaattgg
    11221 attagatgga aaacgggatg tggggaaact cgcccgtagg tgtgaagtga ggggaaaacc
    11281 ggtgataaag taaaaagctt acctaacact atagtaacaa agaaagccca attatcaatt
    11341 ttagtgctga ggaattggtc tctttaataa atttccttaa cgttgtaaat ccgcattttc
    11401 ctgacggtac ccctacgaga gcgattttga gccattttca aacgtaatat tgaataacta
    11461 tccattaaaa tccttacaca gcacgattta aagtagacaa agaaaataaa aggaaagccc
    11521 tgctaatgaa atttattagc agggcttttt agtaagaaaa tatttgaacc atttttacag
    11581 atttagccta tacgtccttt cagttatttt taagtgcgta tatgtcgttt gcttttagcg
    11641 ttatcgcaaa taagaaccta ttcctgcaag ggtaaaatgc acgatcagga atcgcccttg
    11701 caggaataga accattattt gcagagagat aactaagcaa accgccaaac gcactgaaaa
    11761 aaataactga aaggaagtga gtagctaaaa atctaaccag taaaaataaa tattttaaaa
    11821 acataaagtg ctaagcaccc aaccaaaaac ctaaaaaaaa ttgtttaaaa aagaaaggaa
    11881 cattaaacat gattacaaaa attgaagata ttacaccact tgaaatcaca ttacaaacag
    11941 aaaattatca tcaagtattg agtgaatatt ttggtgaagc atactggaca agcgaatttg
    12001 aagtcgaaga aatagatatt gaggaaaata aaaaaatctt tgctgtttac aacaatgaac
    12061 tcgatgagat ttgtcagtta ggtattatca cactcacata tgaaaataaa aaaataaaat
    12121 ccatacatct attaagagaa atactttagg aggaaaaata atgactgatg ttatgattta
    12181 taaccaccaa aaatttatta agacaattcc aggcgataca aaaagtatcg ctaaggaagt
    12241 tgcaaaacaa gacttttcag cacgtttaac aattactgat gt
//
LOCUS       DACECB010000079        12281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECB010000079 DACECB010000000
VERSION     DACECB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.079"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..1042)
                     /locus_tag="IWO04_002389"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IWO04_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IWO04_002390"
     CDS             complement(1437..2279)
                     /locus_tag="IWO04_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0213962.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IWO04_002391"
     CDS             2604..3020
                     /locus_tag="IWO04_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0213963.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IWO04_002392"
     CDS             3017..3487
                     /locus_tag="IWO04_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0213964.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IWO04_002393"
     CDS             3501..4274
                     /locus_tag="IWO04_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0213965.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IWO04_002394"
     CDS             4288..5100
                     /locus_tag="IWO04_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0213966.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IWO04_002395"
     CDS             5154..6119
                     /locus_tag="IWO04_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0213967.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IWO04_002396"
     CDS             6256..8979
                     /locus_tag="IWO04_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0213968.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IWO04_002397"
     CDS             8992..9624
                     /locus_tag="IWO04_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0213969.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IWO04_002398"
     CDS             9829..10056
                     /locus_tag="IWO04_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0213970.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IWO04_002399"
     CDS             10125..10391
                     /locus_tag="IWO04_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0213971.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IWO04_002400"
     CDS             10381..10650
                     /locus_tag="IWO04_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0213972.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="IWO04_002401"
     CDS             complement(10829..12130)
                     /locus_tag="IWO04_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0213973.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4006 a         1989 c         2409 g         3877 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
    12181 ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc tatcagtcct
    12241 agaaattata gagccttttt tatttggtct tgaagaagaa a
//
LOCUS       DACECB010000080        12171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECB010000080 DACECB010000000
VERSION     DACECB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.080"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            335..1246
                     /locus_tag="IWO04_002402"
     CDS             335..1246
                     /locus_tag="IWO04_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0213974.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1463..1900
                     /locus_tag="IWO04_002403"
     CDS             1463..1900
                     /locus_tag="IWO04_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213975.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            1995..2879
                     /locus_tag="IWO04_002404"
     CDS             1995..2879
                     /locus_tag="IWO04_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0213976.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3103..6270
                     /locus_tag="IWO04_002405"
     CDS             3103..6270
                     /locus_tag="IWO04_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213977.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6290..6835
                     /locus_tag="IWO04_002406"
     CDS             6290..6835
                     /locus_tag="IWO04_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0213978.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6863..7318
                     /locus_tag="IWO04_002407"
     CDS             6863..7318
                     /locus_tag="IWO04_002407"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0213979.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7315..8073
                     /locus_tag="IWO04_002408"
     CDS             7315..8073
                     /locus_tag="IWO04_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0213980.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8159..8899)
                     /locus_tag="IWO04_002409"
     CDS             complement(8159..8899)
                     /locus_tag="IWO04_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0213981.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8960..10213
                     /locus_tag="IWO04_002410"
     CDS             8960..10213
                     /locus_tag="IWO04_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0213982.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10535..10762
                     /locus_tag="IWO04_002411"
     CDS             10535..10762
                     /locus_tag="IWO04_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0213983.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10885..11211
                     /locus_tag="IWO04_002412"
     CDS             10885..11211
                     /locus_tag="IWO04_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0213984.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11279..11992
                     /locus_tag="IWO04_002413"
     CDS             11279..11992
                     /locus_tag="IWO04_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0213985.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         4219 a         2020 c         2514 g         3418 t
ORIGIN      
        1 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
       61 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      121 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      181 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      241 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      301 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      361 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      421 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      481 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      541 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      601 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      661 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      721 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      781 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      841 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      901 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
      961 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1021 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1081 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1141 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1201 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1261 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
     1321 caacagtagt ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt
     1381 agaatccagc gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa
     1441 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
     1501 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
     1561 tttgtatata aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc
     1621 ggggattgtc gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt
     1681 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
     1741 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
     1801 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
     1861 cagtttcata gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt
     1921 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
     1981 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
     2041 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
     2101 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
     2161 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
     2221 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
     2281 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
     2341 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
     2401 tcgcaattgg ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat
     2461 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
     2521 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag
     2581 gactaactaa ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
     2641 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
     2701 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc
     2761 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
     2821 gggataaatt acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa
     2881 atggaggttg tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt
     2941 gacaagactt ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa
     3001 aatttttcca taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac
     3061 gataaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa
     3121 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
     3181 gatgtagaga aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact
     3241 ttttcagaag aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat
     3301 aaaaggcaag aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat
     3361 agcagtgaag cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca
     3421 acagaagaac tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag
     3481 cctttgactt tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga
     3541 ttcgctcaca atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag
     3601 ttccagaatt ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga
     3661 aatgccatta tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga
     3721 gaaataaaat ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac
     3781 gctcctcgtg caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg
     3841 aaggaaggac tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat
     3901 gtgatcaatg gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat
     3961 tcaggagatg agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca
     4021 aaggtctctc ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat
     4081 acgctacctg gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat
     4141 acaaagatca atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga
     4201 tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga
     4261 tttcgcttgc aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc
     4321 aaaatggctg gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc
     4381 ctttctttga tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt
     4441 tccagcgcag ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca
     4501 ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat
     4561 caagcgatcc aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt
     4621 acagtcactg gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat
     4681 acaactggag atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc
     4741 gggaaaacag gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta
     4801 gaatctccta cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata
     4861 ggcggctggg tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc
     4921 gctttatggc gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt
     4981 tctgatatga cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct
     5041 agtgcaggtt taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga
     5101 atcagtgcca ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat
     5161 aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct
     5221 tggacaggcg aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct
     5281 gtagaaatta ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac
     5341 ccatggggag aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga
     5401 ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa
     5461 gtgacagaac tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat
     5521 ttaagtacca cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga
     5581 gccttggttt cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca
     5641 ggggaatttg ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt
     5701 gtattaaacg accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc
     5761 aacgatgaaa ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg
     5821 ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc
     5881 aaactcgatt ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg
     5941 gatatacagg aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta
     6001 acgataaaat tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta
     6061 tatttcaaga aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga
     6121 ggacaaaatt caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg
     6181 tttaaacttg tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca
     6241 tggacattag cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat
     6301 atggaataaa agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat
     6361 cctttggcag acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc
     6421 ggctaatcag attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaac
     6481 acaggaaatc ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt
     6541 gatccactcc gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa
     6601 atttgatgaa acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac
     6661 tgttcctcct caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct
     6721 ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa
     6781 aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag
     6841 cgaaaaagag tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg
     6901 aagaaatgtg attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac
     6961 tacatcggtt gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga
     7021 aaaaatagat gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa
     7081 attagctccg ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa
     7141 tctccatacc gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat
     7201 tgatttaaaa aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat
     7261 tcttattggc ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa
     7321 aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt
     7381 tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat
     7441 gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact
     7501 ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct
     7561 ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta
     7621 caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg
     7681 gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt
     7741 acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt
     7801 cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa
     7861 ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg
     7921 attggcacat ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa
     7981 ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg
     8041 ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg
     8101 agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc
     8161 aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc
     8221 gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt
     8281 tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa gcatactttt
     8341 ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct gtagttgttt
     8401 tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt tcccgaggat
     8461 acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt acgatctgct
     8521 gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa ttcctgtctt
     8581 ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca aaattcgtat
     8641 aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt tcggcgacat
     8701 caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa
     8761 tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc acgatattct
     8821 cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg ttatcttcta
     8881 tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac attttgaaac
     8941 aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct ttactttgat
     9001 tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa acagctttgg
     9061 ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga gctttcctta
     9121 cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc tgtttatcag
     9181 ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc tggtttttga
     9241 tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg cactacttat
     9301 cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat ttctcaactt
     9361 gctttttact tatccaactg aacactattt tggaggtatt tgtattttat taagctgggg
     9421 cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc tgcttctatc
     9481 tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt tgatcagttt
     9541 ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa atgaagaata
     9601 ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt cattaaaaat
     9661 tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag aagatcattc
     9721 tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat ccccagctgt
     9781 caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt
     9841 agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta ttcgctgttt
     9901 atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc cgattacgat
     9961 caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg caaatccaac
    10021 gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt
    10081 aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac taaaaatcga
    10141 attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta gaaagagcaa
    10201 atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac ggagatagag
    10261 acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca cccttctgtt
    10321 tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta ttagtacgaa
    10381 agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa
    10441 tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat tttaggcgtg
    10501 gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt taagagagtt
    10561 agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta aagtagagta
    10621 ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt ctgtttggga
    10681 agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac tgcatacaca
    10741 tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa gaaaacagga
    10801 gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta atcaagtaag
    10861 aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca gtttagagat
    10921 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat ttctttggaa
    10981 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
    11041 ttagaacgaa tcaaagaaga actggcagat gtattgatct attcatacat gatggcagac
    11101 aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
    11161 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta gaaatgtgtc
    11221 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
    11281 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
    11341 agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca gagagacagg
    11401 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
    11461 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
    11521 aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
    11581 tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
    11641 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
    11701 tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct atttgacaga
    11761 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
    11821 ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt gtggcgacat
    11881 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat cgataaaaga
    11941 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
    12001 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
    12061 tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt tttctagata
    12121 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat a
//
LOCUS       DACECB010000081        12109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECB010000081 DACECB010000000
VERSION     DACECB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.081"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWO04_002414"
     CDS             51..260
                     /locus_tag="IWO04_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0213986.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWO04_002415"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWO04_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IWO04_002416"
     CDS             complement(1379..1984)
                     /locus_tag="IWO04_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0213987.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IWO04_002417"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IWO04_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IWO04_002418"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IWO04_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IWO04_002419"
     CDS             3019..3990
                     /locus_tag="IWO04_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0213988.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IWO04_002420"
     CDS             4130..5443
                     /locus_tag="IWO04_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0213989.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IWO04_002421"
     CDS             5486..6352
                     /locus_tag="IWO04_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0213990.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IWO04_002422"
     CDS             6349..7197
                     /locus_tag="IWO04_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0213991.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IWO04_002423"
     CDS             7211..8683
                     /locus_tag="IWO04_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0213992.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="IWO04_002424"
     CDS             8700..9572
                     /locus_tag="IWO04_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0213993.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="IWO04_002425"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="IWO04_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..11933
                     /locus_tag="IWO04_002426"
                     /pseudo
     CDS             11652..11933
                     /locus_tag="IWO04_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12007..>12109
                     /locus_tag="IWO04_002427"
     CDS             12007..>12109
                     /locus_tag="IWO04_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0213994.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT         3765 a         2062 c         2436 g         3846 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat ttcggttctg
    11941 ttgcaaagtt ttccaaaaaa tctattttag tgtaaaattg agaaaaaaga cagagaggac
    12001 agagtaatga atcattttaa aggcaaacaa ttcaaaaaag acgtcattat tgtcgctgtt
    12061 ggttactacc tgcgttacaa tctaagctat cgtgaagttc aggaattgt
//
LOCUS       DACECB010000082        11833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECB010000082 DACECB010000000
VERSION     DACECB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.082"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(269..802)
                     /locus_tag="IWO04_002428"
     CDS             complement(269..802)
                     /locus_tag="IWO04_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0213995.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1543..2295)
                     /gene="xth"
                     /locus_tag="IWO04_002429"
     CDS             complement(1543..2295)
                     /gene="xth"
                     /locus_tag="IWO04_002429"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0213996.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2408..3328
                     /locus_tag="IWO04_002430"
     CDS             2408..3328
                     /locus_tag="IWO04_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0213997.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3401..4336
                     /gene="murB"
                     /locus_tag="IWO04_002431"
     CDS             3401..4336
                     /gene="murB"
                     /locus_tag="IWO04_002431"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0213998.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4634..6112
                     /locus_tag="IWO04_002432"
     CDS             4634..6112
                     /locus_tag="IWO04_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0213999.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6389..8449
                     /locus_tag="IWO04_002433"
     CDS             6389..8449
                     /locus_tag="IWO04_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214000.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8532..9101
                     /locus_tag="IWO04_002434"
     CDS             8532..9101
                     /locus_tag="IWO04_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0214001.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9276..10082)
                     /locus_tag="IWO04_002435"
     CDS             complement(9276..10082)
                     /locus_tag="IWO04_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214002.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10220..11734
                     /locus_tag="IWO04_002436"
     CDS             10220..11734
                     /locus_tag="IWO04_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0214003.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         4133 a         1997 c         2314 g         3389 t
ORIGIN      
        1 tgtaaacggt gtccaaaagc ttgcatctgc ggtaataagt ccaaccaaat gaaaaatatg
       61 agaagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      121 ctccaataaa cggtgttctc ctgccagctc ctcggtcacc agtcacaata tttaataaac
      181 atgagaagct attttttgaa attgctcctt gtgactcgga gtgaaacggc aggctcacga
      241 cctctttgtg tgtttcataa gtattctttt actttatagt cacaagtttt ttcaatggat
      301 ctgtcccgaa ttttttttct tgataaagca ggatttccgc acatgcacga ctatcttcca
      361 atgcatcatg gtgatgatct aattggatgt tcaaattttc gcacacagtg ttcaatcgat
      421 gatttggaaa ttcaggaaac aggcgacggc tacttttcac tgtacataaa gaaagataat
      481 taggctgttc aagatgataa taatctaagc atcccgctaa gacctttgta tcaaaagcag
      541 cattatgtgc aacgactaaa cgattttttt gaaaatatgg ttggatagtt tgccagactt
      601 ctgggaattt cggcgcattg cgtacgtctt cttgatggat tccgtgaatt tggacatttc
      661 gccagaaaaa aggagtctca ggctgtatca atgtatagaa ttcatcaacg atctggctat
      721 tttttaccat gactagcgca agagaacaag cactataagg ctgatgattg gcagtttcaa
      781 aatccatggc aacaaaattc atttctgtaa aacctccttg ggaaattact gtaacatact
      841 tttgaaaaaa gcaaataaat ttaacaaaaa acatagaatt ttttatgtaa tatttgttga
      901 gaaaagctag tttttgaact cattccttgt gatttggagt tagatgggga tcatgacttc
      961 tcggtattaa gtcaaattta tgcaaagttg tgaggaacca ttttcaaaaa aatttcttac
     1021 gactcgaaag taggcgactt tttcacaact tctccactaa acggtgttca atcagctgac
     1081 cctcggtatt agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct
     1141 aatactcagg tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc
     1201 atctgcggta ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
     1261 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
     1321 tgtatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
     1381 gttcttatta cttgatgcag gacagctttt tcacaatctc tcgataaaca gtgttcaaaa
     1441 gccagtttct cggtattatg ccaaatttat gcaaaatgtg gaaagccatt ttcacaaaat
     1501 ttttcttaat actcgaaact aaacgacttt ttcacaacct ctttatttca aatctaattc
     1561 tactggacaa tgatcactgc ccataatgtc cgtatggatc tttgcatcag ctagtcctgg
     1621 ctctaaatct ttgctcgtca aaaagtaatc gatccgccaa ccagcattgt ttttccttga
     1681 attgaatcgg tagttccacc aagagtagac accttcctgg gttggataaa aataacggaa
     1741 tgtatcgata aacccgttat ccaacagtcg agaaagagcc gctctttctt ctggagtgaa
     1801 tccagcattt ttttgattcg ttttccagtt tttcaaatca atcttttgat gagcgacatt
     1861 tagatcccca cacacgatga ctggtttttg tttttttaga ttttccaaat aattataaaa
     1921 ggcttcttcc cattctaatc ggtaatctaa acgttttaat tcgctttgag aatttggcgt
     1981 gtaacaggtt acaagaaaga aatcggaata ttccaaagta atcagacggc cttctgtatc
     2041 atggatatcg atacccattc cgtaagtggc attcaatgct ggttttttag caaaaatcgc
     2101 tgtgccggaa tagccttttc gttccgcgta attccaatat tgataataac ctggaagatc
     2161 taaatcaatt tgtcctgctt ggagtttcgt ttcctgcaga cagaaaaaat cggcgtctaa
     2221 ctcatgaaaa acttctaaaa aatttttatt tacgatggca cgaagcccgt ttacgttcca
     2281 agagataaat ttcaataaaa gcacactcct tcgaattaat ttccttacct ttgcgatact
     2341 attgtacctc attttagtaa aaaatggtgt aagatgaaag taactaaatg atccaggagg
     2401 aaagtagatg aagattggtg taatcggtgt agggaatatc gcagaaaaag catatttacc
     2461 aacttatgcg aaaaaacaag gaacacttga tttttatttt gcaacaagaa ataaagaaac
     2521 aaaagaaaga attcaaaaag agtacgggtt ccaacatcta tatgaaacga tcgatgagct
     2581 tttagaagaa aaaatcgaag catgtatgat ccacgcagca acaaaagtac attatgaatt
     2641 agccaaaaaa tgtctagaaa atggtatcca tgtttatatt gacaaaccac tgagtgtcca
     2701 tctaaatgaa gttcgtgaat tgcaagaatt agctgataag aaccaaataa ttttgatgat
     2761 cggtttcaac cggcgctttg ctcctatggt agaagaattg aaacgtatcc cagataagcg
     2821 gttgatccac ctgcaaaaaa atcgaatagc ggcaaaagaa acgacagagt tcgtccttta
     2881 tgatttgttt ttacatctta ttgatacagc tgtgtattta ttagatgaac ccattttgag
     2941 aacaaaatac cagataagag aaacaaagga atttttggaa tatgccattt tacaattaga
     3001 aacacaaaat caaacggcta ttttaacgat ggatttagca agtggcgcta atatagaaaa
     3061 atatcaagta acaagtaaaa atggtactta tgaagtaaac gatctgaccc agcttgtcat
     3121 tcaagatgct gatggaaaaa aagtgaaaga atttggcgac tggacaaaca cgctagtcaa
     3181 acgcgggttt gagccaatgg tagaggcctt cttttctcag atccgtttag gtaaatcgga
     3241 aacagaattg cttaaacaaa aagaagttgt aagaagtcat gaattatgtg aggaagtgct
     3301 taaagatcac taccgtcatc aactttgaac aagactggca agattgtatt aagcgtgata
     3361 gaataatgag gacgtttgag aaaagaaagg aaaatcctca gtgaataaag aagaaatagt
     3421 tgaaagctta gccacgatta ctatattgtt tgatgaacca ttgatgaact atacgtttac
     3481 gaaaacaggt ggaccagctg atgtccttgc attcccgaaa aaacaagaag aagtaaagca
     3541 aatcatcgat tattgccgaa tccatgatat accttggatg gtattaggaa atgcaagcaa
     3601 tctgatcgtt caagatggcg ggattcgcgg tgtagtcatc atgctgactg aaatgaaaca
     3661 gattcatgtc aaaggaacga tggtcattgc ggaagcaggt gcctcactga ttgatacgac
     3721 ttatgcagct ttggcagaat cattgactgg atttgaattt gcttgcggca tcccgggaag
     3781 tgtcggtgga gcggtttata tgaatgcagg tgcgtatggc ggagagatca aagatgtttt
     3841 tgctgaagtg gatcttttgc ttgaagatgg aacattgaaa actttgacaa aagacgaaat
     3901 ggtattttct tatcgtcaca gtaaagtcca agaattacgt gctatcgtat tggaagctcg
     3961 attcagtttg caaacagggg attatgaagc aatcaaagca agaatggatg agttgaccga
     4021 attgcgtcaa tcaaaacagc cattagaata cccttcatgc ggaagtgtct ttaagcgccc
     4081 agtaggacac tatacaggtc agctgatcca acaagcagga ttgcaaggcc tgaaatgggg
     4141 cggtgcacaa gtctctgaaa aacatgctgg attcatcgtc aatatcgatc atgcgacagc
     4201 gacagattat attgaattga ttgcgcacat ccaagaagta atcaaagaaa aatttgatgt
     4261 gtctttagaa acagaagtgc ggatcattgg agaaaaacaa gcaatatcag aaacaagcag
     4321 aagtttagcc aattaataag taatggaact taaaataagt aaaagagcag gacggtggcc
     4381 gtcctgctct tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca
     4441 ataaaacaaa aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca
     4501 aaaaaaagat aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc
     4561 agtataggtg aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt
     4621 tgaggtgtag gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct
     4681 gctagctaga tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga
     4741 aacattaggt ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt
     4801 tactgaattc aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga
     4861 acgtctgaat ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat
     4921 tgctcgactg ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa
     4981 tcatttttta agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga
     5041 acagcatcaa ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa
     5101 cgtattattt ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag
     5161 cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact
     5221 gtctgcaaca aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta
     5281 tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg
     5341 aatgaagatc atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat
     5401 cattgatgag atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga
     5461 tggcgcattt ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat
     5521 gtcagtggaa ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact
     5581 atatatacga aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt
     5641 cgactttgag atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta
     5701 tccaatattc agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg
     5761 aaaagatgaa cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat
     5821 ttgtccggta tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc
     5881 tgcttatacc gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat
     5941 tgagagtcga atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt
     6001 taataaagaa cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg
     6061 aaataccatc atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg
     6121 gatggcagct ttgtctcaca tatttattag ctgatttatg tttttttcaa agaaaaggta
     6181 actagatata actagatata actagatata ttacttaaca aaattaaaaa gtacataagt
     6241 tattattccg attttcttga aacaataaaa aatcaaaaaa aatactctcc taatagtcca
     6301 tcttcttttg attttattta tataatgaaa tcatcttatg aaaattacta cttcatgttt
     6361 agacaagagt agggaggact aagaataaat gtgttcaaaa agacgttcaa aacaactaaa
     6421 taaacacgct tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact acgttaagtt
     6481 tatgatgcgc acgatgctga tcagtttatc tgtaggtgtc tatatgcatg tacccgttca
     6541 ggcaacgaca caaaccatca aggtcgtacg tacctctgtt tctgagacca ctgattcccc
     6601 tatcactatt actcatactt tcactgatgc aaatggaaaa cagctacttc ctgcattgac
     6661 ttcctataat aaagtcagtt atcctccaac gataaaccac tactggttaa ttcctaacaa
     6721 aacattatgg acatcggaaa ccggaagcag tgtgccgctt tctatctttt ccgatatgac
     6781 tgatcctaca aaaggtgatc agttaaaaca gatcactgat attttgaatc atgctttgac
     6841 agatataaag gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag attattcaca
     6901 gtttgaaaga aagaaaatcg atgttctggc agggacaaaa gtttcgatat ctgatctggt
     6961 agaaaggctg aagaacgcag atggaacaca aggagatatg tccaaagtaa tttcaaacaa
     7021 tgggaatatc gtggatacga ctaatacagg aacatctact atttccttgt cctattttga
     7081 tccaattgct ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac
     7141 agctatcaaa ggaaaagatc ttcacttaag aacaggagag agttacgccc ctaaagatct
     7201 tgttgaatat gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa
     7261 cgggacagta gatactacaa ctccgggaga atatcccgtt gtattaactt atatagactc
     7321 gaaaacatca aatgtggcag aaacaacagc tgtagtttat gtagaagcat ctgctgcttc
     7381 atcgactgtc atcactcatt ttatagatag tcaaacaaat aaagaacttc aagcagaaac
     7441 acatatgagt agtgtaaatc gtattcctga tttgcgccta cctgctgaat tttctgatcg
     7501 agtgccaaat atagatttat cgacttatga acaaaatggg aaagtcactt ctatttctga
     7561 ttggatgcaa gcaaacggat tagacagaaa aagtcactgg tcggagatcc ttgaagtact
     7621 tcactcagaa ctgatgaata ataggaagca atcaggaggg gaaattacct ttacttatgt
     7681 ttatatacca gacaatcgtt cattagaagg tcatgatctt attgttcatc caaacgaaaa
     7741 aataaacaaa gaagagttga tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt
     7801 aaaggatgta tcggttacaa tcaatgaagt accattggag gatggttatt tgaccaaaca
     7861 aacaggtgat tttacgatta tgtatagtta tcatgacccg tattcactcc aagaactcca
     7921 tgctcagtca ctattacatg ttttaccatt aaacaaacag atagatcaaa aagaacaaaa
     7981 aagacaagaa gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag tttctccaac
     8041 agggaaagta aaacaaacaa gaagcctaga gttagcaaga atatctagaa aaatctcaga
     8101 atctaataaa acgataaggc aagaagctca tttggcccca caagtttctg gattggaaaa
     8161 tgaaatttta gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc
     8221 ggaagatcag aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat
     8281 tgattccacc gatgagcatc tgcagccgca agtaccactt cctagtccgg gaacacccgc
     8341 acctggtgga ggtattcctt cttctgcttt aggagatttc atgcgaggta tggcaggaac
     8401 atgggtatac gctaatcgaa atgaatttga cgcagatgac tggttctgat ttatatctct
     8461 atctgttata gtcaaataag tagctgagtc atatgttatt tggcataatg gagacgagga
     8521 gggatagaaa aatgttagat aaaacgttac cttacgcaga aatctggatg acaagaccac
     8581 ttcaacaaaa tgttccaatc acgcctttag cggaaggttt tgaatttcga ttttatcaaa
     8641 aagaagatga atctgcttgg gcggagattg agacttccgt tggggaattt caaacgacag
     8701 atgaagcact tgagtatttt caacaaaact ttgctcctta tcctgaagaa cttgaaagaa
     8761 ggatgttttt tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa
     8821 aaaaatcaag agagcaagtg tatccggttt ttcattggtt agcagtgaaa ccagattacc
     8881 aaggaaaagg gctcgcaaaa gcattgactg ccagagtctt gcaagaattt cctaccctcc
     8941 atacggacgg accaatctat cttcacacac agacatggag ccatcaggcg attgctcttt
     9001 ataaaaaaat gggttttact tttattgctg aaaatctaga cggcacacct aatccagatt
     9061 atgaaaaagt tatgcaggtt ttaagtaaat tagataaata acgaaatggt gaaataagtc
     9121 ctcagtattg ggataaaaaa cgaaatagtc attcctcata tcttactttt ttgagttaat
     9181 actatcggat aactaatggc atgccataca ttcaaaaaag aaggggcagt gacaaaagta
     9241 ttgtcactgc cccttctctt gttttttagc atgttttatc gtttgatttt tttaggaata
     9301 aaattcttga tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta accaaataca
     9361 ctgaaaacaa ctgctccaat aaaattaaca aataaatcct tcatagtatc catgattccc
     9421 acgtctaagt aaccattttg gatgattgtt ttatctgttt taccatctgc attttgactt
     9481 tcaatgattg tccgagtgat atctggaaca tgatagacag tgtttccgtc tttacttaac
     9541 tccacactat tgatttgctg gatgatcgta tctttttgca tatcgaattg cagtaattta
     9601 tctgctgaaa attccacaaa ctcccacata acaccaactg tcatcgaaaa acagaaggaa
     9661 acaactgcta gtaataaagg agtcaagttc atggtttttg tatttttatt gagcaaataa
     9721 acaagggaaa agccgacacc cccagctaaa aatccgctga atgcatgtag aaaagtatcc
     9781 cacattgaaa aatagccata aaaatcactt aactctccta aaactgttga actaaaaata
     9841 aaagataaca aaatgatttc tagcatattt ggaatctgta tctttaatat cttttccaga
     9901 aaataaggga tttcaaataa aattaatgtc agtaataaaa gcattgtatt tccccagtgt
     9961 tggttccaga cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt
    10021 ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc
    10081 ataaaataac tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc
    10141 tatatgactt aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa
    10201 ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact
    10261 tcttgctatg agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac
    10321 gaaggaatct gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga
    10381 aatgaaagaa tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc
    10441 tatcgaagca aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg
    10501 aggaaacaca tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga
    10561 agaaggaatc aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg
    10621 aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg
    10681 gctagatcaa aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa
    10741 gaggacacat cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt
    10801 gaataacgtc catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa
    10861 gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga
    10921 ggtcaccgga aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat
    10981 tgaaaaatat cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg
    11041 agatggtatc accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca
    11101 aattcatcca actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga
    11161 aggagcaatc ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga
    11221 taaagtatct gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca
    11281 aggcgtgcgt gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac
    11341 tggaaagaca atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa
    11401 aacagttgat ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc
    11461 cacagccatg gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg
    11521 aatccattac acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga
    11581 aaaccagcca attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa
    11641 caaccggatc ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat
    11701 acaagcagct cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg
    11761 tgaaatcagc gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga
    11821 ggttgtgaaa aag
//
LOCUS       DACECB010000083        11587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECB010000083 DACECB010000000
VERSION     DACECB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.084"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..1247)
                     /locus_tag="IWO04_002437"
     CDS             complement(117..1247)
                     /locus_tag="IWO04_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0214004.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1788..1994)
                     /locus_tag="IWO04_002438"
     CDS             complement(1788..1994)
                     /locus_tag="IWO04_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214005.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2010..2624)
                     /locus_tag="IWO04_002439"
     CDS             complement(2010..2624)
                     /locus_tag="IWO04_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0214006.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2771..3454)
                     /locus_tag="IWO04_002440"
     CDS             complement(2771..3454)
                     /locus_tag="IWO04_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0214007.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3563..4021)
                     /locus_tag="IWO04_002441"
     CDS             complement(3563..4021)
                     /locus_tag="IWO04_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0214008.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4105..5868)
                     /locus_tag="IWO04_002442"
     CDS             complement(4105..5868)
                     /locus_tag="IWO04_002442"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0214009.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6089..7549)
                     /locus_tag="IWO04_002443"
     CDS             complement(6089..7549)
                     /locus_tag="IWO04_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214010.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="IWO04_002444"
     CDS             complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="IWO04_002444"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0214011.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8706..10133)
                     /locus_tag="IWO04_002445"
     CDS             complement(8706..10133)
                     /locus_tag="IWO04_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0214012.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10164..11378)
                     /locus_tag="IWO04_002446"
     CDS             complement(10164..11378)
                     /locus_tag="IWO04_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0214013.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3505 a         2403 c         1912 g         3767 t
ORIGIN      
        1 atatgatttt ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg
       61 aaaaataaaa gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat
      121 tgtttttcag gtcgatagag cgccaactct tcggcctgta tctttgaaga aatattcaac
      181 gtgattccga ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc
      241 aatggcacac cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga
      301 acgaatagca aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga
      361 ctgcttaaac aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca
      421 ccgattagcc caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga
      481 agataccctt tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat
      541 agtgcataaa acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct
      601 cttaaagtcg cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt
      661 cctaaatata ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga
      721 tagatccctt ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg
      781 atcatcaatg ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta
      841 atcaggaatg gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt
      901 aaataaaaaa tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga
      961 gagatccaac gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact
     1021 aaaaagaaaa tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt
     1081 agtttgactg aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga
     1141 cgcaataata gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg
     1201 tatacttcta aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg
     1261 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     1321 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     1381 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     1441 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     1501 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     1561 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     1621 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     1681 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     1741 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     1801 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     1861 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     1921 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     1981 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     2041 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     2101 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     2161 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     2221 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     2281 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     2341 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     2401 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     2461 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     2521 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     2581 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     2641 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     2701 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     2761 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     2821 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     2881 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     2941 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     3001 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     3061 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     3121 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     3181 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     3241 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     3301 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     3361 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     3421 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     3481 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     3541 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     3601 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     3661 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     3721 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     3781 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     3841 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     3901 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     3961 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     4021 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     4081 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     4141 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     4201 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     4261 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     4321 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     4381 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     4441 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     4501 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     4561 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     4621 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     4681 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     4741 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     4801 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     4861 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     4921 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     4981 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     5041 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     5101 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     5161 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     5221 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     5281 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     5341 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     5401 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     5461 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     5521 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     5581 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     5641 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     5701 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     5761 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     5821 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     5881 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     5941 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     6001 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     6061 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     6121 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     6181 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     6241 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     6301 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     6361 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     6421 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     6481 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     6541 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     6601 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     6661 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     6721 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     6781 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     6841 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     6901 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     6961 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     7021 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     7081 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     7141 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     7201 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     7261 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     7321 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     7381 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     7441 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     7501 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     7561 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     7621 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     7681 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     7741 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     7801 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     7861 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     7921 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     7981 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     8041 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     8101 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     8161 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     8221 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     8281 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     8341 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     8401 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     8461 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     8521 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     8581 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     8641 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     8701 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     8761 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     8821 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     8881 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     8941 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     9001 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     9061 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     9121 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     9181 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     9241 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     9301 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     9361 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     9421 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     9481 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     9541 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     9601 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     9661 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     9721 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     9781 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     9841 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     9901 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     9961 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    10021 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    10081 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    10141 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    10201 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    10261 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    10321 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    10381 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    10441 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    10501 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    10561 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    10621 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    10681 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    10741 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    10801 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    10861 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    10921 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    10981 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    11041 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    11101 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    11161 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    11221 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    11281 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    11341 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    11401 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    11461 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    11521 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    11581 ttttttt
//
LOCUS       DACECB010000084        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECB010000084 DACECB010000000
VERSION     DACECB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.085"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO04_002447"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWO04_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0214014.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO04_002448"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWO04_002448"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0214015.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWO04_002449"
     CDS             complement(2169..2906)
                     /locus_tag="IWO04_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0214016.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWO04_002450"
     CDS             complement(2997..3524)
                     /locus_tag="IWO04_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0214017.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWO04_002451"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWO04_002451"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0214018.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWO04_002452"
     CDS             complement(4401..4886)
                     /locus_tag="IWO04_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0214019.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWO04_002453"
     CDS             complement(5039..5236)
                     /locus_tag="IWO04_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214020.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO04_002454"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWO04_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0214021.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO04_002455"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWO04_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0214022.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWO04_002456"
     CDS             9211..9423
                     /locus_tag="IWO04_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0214023.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWO04_002457"
     CDS             9425..11104
                     /locus_tag="IWO04_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0214024.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACECB010000085        10811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECB010000085 DACECB010000000
VERSION     DACECB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.086"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            34..657
                     /locus_tag="IWO04_002458"
     CDS             34..657
                     /locus_tag="IWO04_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214025.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="IWO04_002459"
     CDS             1107..2156
                     /locus_tag="IWO04_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0214026.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="IWO04_002460"
     CDS             2153..3034
                     /locus_tag="IWO04_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0214027.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="IWO04_002461"
     CDS             3037..3807
                     /locus_tag="IWO04_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0214028.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="IWO04_002462"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="IWO04_002462"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0214029.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="IWO04_002463"
     CDS             5118..6281
                     /locus_tag="IWO04_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0214030.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="IWO04_002464"
     CDS             6398..6808
                     /locus_tag="IWO04_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0214031.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="IWO04_002465"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="IWO04_002465"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0214032.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9348..9665
                     /locus_tag="IWO04_002466"
     CDS             9348..9665
                     /locus_tag="IWO04_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214033.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9825..10061
                     /locus_tag="IWO04_002467"
     CDS             9825..10061
                     /locus_tag="IWO04_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0214034.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="IWO04_002468"
     CDS             10058..10456
                     /locus_tag="IWO04_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0214035.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3779 a         1793 c         2266 g         2973 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatatat
    10681 attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat
    10741 cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct
    10801 tacttttttt g
//
LOCUS       DACECB010000086        10496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECB010000086 DACECB010000000
VERSION     DACECB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.087"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..360)
                     /locus_tag="IWO04_002469"
     CDS             complement(<1..360)
                     /locus_tag="IWO04_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0214036.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IWO04_002470"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IWO04_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0214037.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IWO04_002471"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IWO04_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0214038.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IWO04_002472"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IWO04_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0214039.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2240..2872
                     /locus_tag="IWO04_002473"
     CDS             2240..2872
                     /locus_tag="IWO04_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0214040.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="IWO04_002474"
     CDS             complement(2874..3779)
                     /locus_tag="IWO04_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0214041.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4517)
                     /locus_tag="IWO04_002475"
     CDS             complement(3864..4517)
                     /locus_tag="IWO04_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0214042.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="IWO04_002476"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="IWO04_002476"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0214043.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="IWO04_002477"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="IWO04_002477"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0214044.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="IWO04_002478"
     CDS             6766..7851
                     /locus_tag="IWO04_002478"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0214045.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="IWO04_002479"
     CDS             7848..8912
                     /locus_tag="IWO04_002479"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0214046.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="IWO04_002480"
     CDS             complement(9161..9964)
                     /locus_tag="IWO04_002480"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0214047.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IWO04_002481"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="IWO04_002481"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="IWO04_002482"
     CDS             complement(10271..>10496)
                     /locus_tag="IWO04_002482"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0214048.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DACECB010000087        10022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.089, whole genome shotgun sequence.
ACCESSION   DACECB010000087 DACECB010000000
VERSION     DACECB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.089"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..722)
                     /locus_tag="IWO04_002483"
     CDS             complement(<1..722)
                     /locus_tag="IWO04_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0214049.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="IWO04_002484"
     CDS             complement(727..1494)
                     /locus_tag="IWO04_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0214050.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="IWO04_002485"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="IWO04_002485"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0214051.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="IWO04_002486"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="IWO04_002486"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0214052.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="IWO04_002487"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="IWO04_002487"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0214053.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="IWO04_002488"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="IWO04_002488"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0214054.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="IWO04_002489"
     CDS             4894..5211
                     /locus_tag="IWO04_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0214055.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="IWO04_002490"
     CDS             5211..6917
                     /locus_tag="IWO04_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0214056.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="IWO04_002491"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="IWO04_002491"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0214057.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="IWO04_002492"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="IWO04_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..9681
                     /locus_tag="IWO04_002493"
     CDS             8782..9681
                     /locus_tag="IWO04_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0214058.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1690 c         2006 g         3162 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DACECB010000088         9504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECB010000088 DACECB010000000
VERSION     DACECB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.090"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            130..951
                     /gene="racE"
                     /locus_tag="IWO04_002494"
     CDS             130..951
                     /gene="racE"
                     /locus_tag="IWO04_002494"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0214059.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            954..2309
                     /gene="rph"
                     /locus_tag="IWO04_002495"
     CDS             954..2309
                     /gene="rph"
                     /locus_tag="IWO04_002495"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0214060.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2310..2828
                     /locus_tag="IWO04_002496"
     CDS             2310..2828
                     /locus_tag="IWO04_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0214061.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2935..3435
                     /locus_tag="IWO04_002497"
     CDS             2935..3435
                     /locus_tag="IWO04_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0214062.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(3715..4635)
                     /locus_tag="IWO04_002498"
     CDS             complement(3715..4635)
                     /locus_tag="IWO04_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0214063.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            4800..5813
                     /locus_tag="IWO04_002499"
     CDS             4800..5813
                     /locus_tag="IWO04_002499"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0214064.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(5923..6678)
                     /gene="fabI"
                     /locus_tag="IWO04_002500"
     CDS             complement(5923..6678)
                     /gene="fabI"
                     /locus_tag="IWO04_002500"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0214065.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            6898..7338
                     /gene="fabZ"
                     /locus_tag="IWO04_002501"
     CDS             6898..7338
                     /gene="fabZ"
                     /locus_tag="IWO04_002501"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0214066.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(7453..8214)
                     /locus_tag="IWO04_002502"
     CDS             complement(7453..8214)
                     /locus_tag="IWO04_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0214067.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            8403..9497
                     /locus_tag="IWO04_002503"
     CDS             8403..9497
                     /locus_tag="IWO04_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0214068.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         3092 a         1671 c         1996 g         2745 t
ORIGIN      
        1 aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca
       61 agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg
      121 aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc
      181 ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt
      241 gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat
      301 acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc
      361 aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc
      421 ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc
      481 attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa
      541 gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt
      601 aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc
      661 actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc
      721 attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt
      781 gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca
      841 ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg
      901 cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc
      961 atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat
     1021 atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa
     1081 tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat
     1141 atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac
     1201 tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag
     1261 acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg
     1321 gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa
     1381 aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta
     1441 gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg
     1501 cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag
     1561 gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa
     1621 aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc
     1681 aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa
     1741 aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg
     1801 actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat
     1861 taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt
     1921 tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac
     1981 aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg
     2041 aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc
     2101 ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct
     2161 ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga
     2221 gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt
     2281 ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg
     2341 gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt
     2401 tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg
     2461 ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc
     2521 aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt
     2581 tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa
     2641 taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta
     2701 gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg
     2761 ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag
     2821 agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc
     2881 atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt
     2941 ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa
     3001 aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag
     3061 gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt
     3121 ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa
     3181 ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa
     3241 ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat
     3301 cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg
     3361 catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc
     3421 gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca
     3481 gcgtaccagt tttgacttta tcttgaaaaa gaggctggga caaaagcgtt taactccaag
     3541 aaataagaag gaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc
     3601 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgggac aaaagtgttc
     3661 tttacttttg tcccacgctc tttttagtga gattcgttgc atcgtttgtc caaattattt
     3721 tccgacagca attggactgt tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata
     3781 ggagctcaag aactcttctt ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt
     3841 acgaatcgca aaattgctgt ttccaagatc cactaatcca aagatagaag gttcagtttg
     3901 gagctcttct tggtattttt cagctaatgt acgatttact cgatcaatga tctcatagat
     3961 tttgtcatat ccttcttcag gaacaatccg aatatctaaa agtacttgca tatttgcacg
     4021 agagagattg ctgatcgtgg tgatgtttcg attcgggatg aaatggacag taccatccgt
     4081 tgctttcagc tgaagggtac ggataccaac agatacgacg gttccttcaa tcgttaagtt
     4141 tgctagcttg atgtagtccc caacatccat ctgttgttcc atgataataa aaaatccggt
     4201 aataacatca ctcatgaacc cttgggcacc aagaccgatt gccacacctg caattccggc
     4261 accagccaaa agtgatccga tcggcacacc gatagcagat aaaattgcat agataaaaaa
     4321 gaaaccaagg gtataatgaa aaatagtatt taaaagacta tgtaaagtct ttaagcggct
     4381 ctcgtttaga atggcttttt tagaatactg attaaaagac ttatcaatca gatattttcc
     4441 cacacggtga agaatccaaa aaaggacgat caaaaaaatc agatataaac ttttttggat
     4501 aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa acgcatttac
     4561 tttgctgaca gcttgatctg tcaatgtctc gccagaagag tctgtcgtac cggcagttgc
     4621 taataatgaa aacataaaat cctcttttct aaaatttgat tctttctgta ttgtaccaag
     4681 aagttttcaa aaaaggaaat agcaaaaata actataatga aggaaaaaag ctgaaaataa
     4741 aatgacaaat aatatggata atgataaaat gtggagaaaa taacgaaggt ggtaagtata
     4801 tgcaaatcga ttggaacaat ctaggttttt cttatatcaa aactccttgg cgttttattg
     4861 ccaaatggaa agacggacaa tgggaagaag gagaattgac agaagataat tacctgacga
     4921 ttcatgaagg ctctccagcg cttcattatg gtcagcaatg ttttgaaggt ctaaaagctt
     4981 atcgtcgaaa agacggcaag gtcaatctct ttcgacctga acaaaatagt cgacggatga
     5041 atcaaagtgc tagacgattg ctgatgccaa tggtccctga aaagatgttc gttgatgctg
     5101 tcaagcaagt ggtaaaagca aatgaagatt ttgtgccgcc ctatggaaca ggcgctacat
     5161 tatacttgcg ccctttactg attggggtcg gtgaaaacat tggtgtccat ccagctccag
     5221 aatatttatt tgtcgtcttc tgtacgccag taggagcgta tttcaaaggt ggactaaaac
     5281 caacgaattt tattgtatct gattacgatc gtgctgctcc taaggggact ggtgcagcaa
     5341 aagtcggcgg aaattacgca gcgagtttac ttccaggaac agaagcaaaa gaactggatt
     5401 actcagattg tgtttatctt gatccagcca cacatacgaa aatcgaagaa gtaggtgcag
     5461 caaatttctt tggaattaca aaagacggaa aattcatcac tccgcaatcg ccctctatct
     5521 taccaagtat cacgaaatat tccttgttgc agttagctga agaacgacta ggactgcaag
     5581 caattgaagg agatattttc attgatgatc tagatcaatt tgcagaagct ggtgcatgtg
     5641 gtacagcggc tgttatttct ccaatcggtg ggatttatca caataacaat cttcatgtat
     5701 tttatagtga aacggaagta ggaccagtaa caaaacggtt gtatgatgaa ttgacaggta
     5761 ttcaatttgg agacatagaa gcaccagaag gctggattga agtcgtggaa taatttcaaa
     5821 gtaatgagtt gaaaaaaacg taaaaagaaa aaatcaggaa gagactttaa aaagtttttt
     5881 cctgattttt atatgtttta ctttaaagaa tgcaacaaaa gtttatgaaa gatggacacc
     5941 tttgtcaacg tagataatat caccgatgat tccgctggaa agtgggctga ttaagaaagc
     6001 acaagcatta ccgacttctt cgatcgtcac accttgctga tcaggcgtac gagattcaga
     6061 taactggatc agtttttgat agtctttaac acctgtgaca gctaatgttt tgattgcccc
     6121 agctgagatt ccattgaccc gaattccttt aggcgagaac tcagctgcca aataacggat
     6181 agcagcttca agagaagctt tcgcaatacc catcatgtta taattcggga ttgcgcgttc
     6241 tgaacctaga taagtcatgc taacgatccc gctgtttggt gcaagaaacg gttgtgcgta
     6301 ttttgataca gcaattaaag agtagctgct gatatcttga gctaattgat agccgtctct
     6361 ggaaatatca gacacatttc cggacaattc ttctttgttg gcgtaagcaa ctgcatggac
     6421 aaggccgtgg atctccccag catattcacc gatggcattc attgcttttt cgatgctttc
     6481 gtcgctagaa acatcacatt ctacagtgaa tgcttcttcg ccgaccaatt tgactaatga
     6541 ttttttcatt cgttcatttt ggtaagtata gataatttct gccccttgtt tcaacattgc
     6601 ttcagcacaa ccccaagcaa tgcttttttt gttagcgacg cccataatga cgattttttt
     6661 tccttctaga aatgacataa tgactctcct ttgtttctga taaaaatttg atagtcgtaa
     6721 aaaaatagag aataatagct aaaataactt cttgatttct taattattag tcaaaatgtt
     6781 ttgattgtca aactattttt tcttaaatta aaaaaagatt gctaaatatt taggaatatg
     6841 atactttgat agtcaaagta atgaaaatgg caattgcctg aaagaaggag tttaattatg
     6901 tcttacgtat tatctgctac tgaagtaatg gaattgattc ctaatcgtta tcctatttgt
     6961 tatattgatt atgtagatac tttagaacca ggaaaaaaga tcgttgcgac aaaaaatgta
     7021 acaatcaatg aagatttttt ccaaggtcac tttcctgaca atccagtgat gccgggtgcg
     7081 ttgattctag aaacactggc acaagcaggt tcgatactta tcctaaaatc agaagagttt
     7141 ctaggaaaaa gagcttatat cggtgggatc aataaggcca aattccgtca aaaagttgtt
     7201 ccaggagatg tgctgaaatg tcattttgaa atcaccaaaa tgaagggatc agtcggtaca
     7261 gcgttatctg cagcatatgt agatgataaa aaagtctgtg agtgcgagtt cacgttcatc
     7321 gtcaaccaaa atgcttgact ataaaacctg atatagcaaa acaaaaggaa gcttacttgc
     7381 tttttatgta aaaagaagac gcctgaatcc tagcatacgt tgtcatctat gcaggattca
     7441 gacgttattt ttttattttt gttttgcttc ttgcagtata ttgattttat cgatttttac
     7501 ttcttctttt ggtttgtctg aactatctgt ttcgatattt gcaatagcat ccacgacatc
     7561 cattccttca atcacttggc cgaagacagt ataatggcca tctagtgaag gataaccgcc
     7621 gtttttataa gcgtcgatga ttgcttgagg atagtcgtct tttaaaagac catcatgcat
     7681 atcatctgta ttttgaacga taaagaactg gctgccgtta ctgttaggat cttgtgagcg
     7741 agccatagat aacgcacctc ttatattata aaggtggttg ttgatctctg tttcaaatcc
     7801 ttctccccaa atgctttccc ctccagtgcc atcgccttta ggatcgccac cttggatcat
     7861 gaaatctttg atgacgcgat ggaaagtcag tccgttgtag taaccgtctt ttgcatgtgt
     7921 cataaagttt tcaaccgctt ttggtgcgtg ttcgggaaat aatttgattt tgattgtgcc
     7981 tttattcgtc accatttcga ccagatcttc attttcggcg acatctttcg tcaattgtgg
     8041 taattccaat gcgttcaaat cgacgctact ggcagtcgtg ctttccgcag tagatgtact
     8101 ttttgaactt tctgtagacg aactagaaga agtcgtgttg tttgcagaac aaccagcagc
     8161 aaacagcaat gctgctgcta aactaatact taaaataact ttatttttct tcataactgt
     8221 tcctttccaa tttactattt tgttatcttt aatgataaca aaatagaggg aaaataccta
     8281 cttaaattat tggaatttat catttatttt aaaagaaaaa gaaaaggtgt agaccattta
     8341 ctttgtgttt gctataatgt aagtggatac aaaaacgaaa agatgcagga ggaaatggta
     8401 atatgggaaa attaggaatc tcgatttatc cagaacggtc aacatttgaa aaggacaaag
     8461 cgtacttgga tttagctcat aagtatgggt ataaacgggt ttttaccagt ttgctgcaga
     8521 tcaatgatga taaagaaaaa gttctttcag aatttaaaga agtagttgat tacgcgaata
     8581 gcctaggcat ggaagtaatg gttgatatca atccagcgtt atttgaacag ttggagattt
     8641 cttacgatga cttatctttt ttccataaga tgggtgccta cggggtccgc ttagatatcg
     8701 gattcacagg agcagaagaa gcaaaaatga cgcgcaatcc ttttggtatc aaaatcgaga
     8761 tcaatatgag ttcgggcacg aattacgttg acaacatcat gagttattcg ccaaacacgg
     8821 ataatttatt aggttctcat aatttttatc ctcatcgata ctctggttta ggatacgaac
     8881 actttgtttt ttgttcagaa aaattcagaa aatataattt gaatacgatg gcattcgtca
     8941 attcgcaatc tgctgagttt ggcccttggc ctacgcaaga tggcttgtgc acgctggaag
     9001 accaccgaga tcttgaaatt gcgacacaag tgaagcattt aatccttaca ggattgattg
     9061 atgatatttc tattggaaat gcgtacgcaa gtgaagaaga attgaaagaa atggcagaag
     9121 catttaacgc ggattatcca acattgaaag tagatacgga agaaggtatt acagaaaacg
     9181 aacgaatctg cttgtttgat aaccttcata gttatcgagg ggatcgctca gaatatatcc
     9241 ttcgttcaac gatgactcgt gtatactaca aagataaaga tttcccgcct cataatacgc
     9301 gagatatgca tcatggggat gtactgattg acaatgaagg gtacggtcaa tacaaaggag
     9361 aaacacaaat cgccttgaag gatatgaaaa acgacggacg tgtcaatgtt gtaggaagaa
     9421 tctccgatga cgaattattc ctccttgact ttttgaaacc atggtcaagc tttaaattaa
     9481 ttgaaaataa aaaatagata atta
//
LOCUS       DACECB010000089         8297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.091, whole genome shotgun sequence.
ACCESSION   DACECB010000089 DACECB010000000
VERSION     DACECB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.091"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..1518)
                     /locus_tag="IWO04_002504"
     CDS             complement(52..1518)
                     /locus_tag="IWO04_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0214069.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="IWO04_002505"
     CDS             1905..2501
                     /locus_tag="IWO04_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0214070.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="IWO04_002506"
     CDS             complement(2559..3041)
                     /locus_tag="IWO04_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214071.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="IWO04_002507"
     CDS             complement(3147..3344)
                     /locus_tag="IWO04_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214072.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="IWO04_002508"
     CDS             3639..4397
                     /locus_tag="IWO04_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0214073.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="IWO04_002509"
     CDS             4457..4885
                     /locus_tag="IWO04_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214074.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="IWO04_002510"
     tRNA            4931..5003
                     /locus_tag="IWO04_002510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="IWO04_002511"
     CDS             complement(5153..5524)
                     /locus_tag="IWO04_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0214075.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="IWO04_002512"
     CDS             5739..5924
                     /locus_tag="IWO04_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214076.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="IWO04_002513"
     CDS             complement(6007..6195)
                     /locus_tag="IWO04_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214077.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="IWO04_002514"
     CDS             complement(6219..6482)
                     /locus_tag="IWO04_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214078.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="IWO04_002515"
     CDS             6640..6897
                     /locus_tag="IWO04_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0214079.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="IWO04_002516"
     CDS             complement(6968..7159)
                     /locus_tag="IWO04_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214080.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="IWO04_002517"
     CDS             complement(7300..7620)
                     /locus_tag="IWO04_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214081.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="IWO04_002518"
     CDS             complement(7643..8077)
                     /locus_tag="IWO04_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0214082.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2634 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacg
//
LOCUS       DACECB010000090         8291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECB010000090 DACECB010000000
VERSION     DACECB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.092"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            433..600
                     /locus_tag="IWO04_002519"
     CDS             433..600
                     /locus_tag="IWO04_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214083.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            674..1900
                     /locus_tag="IWO04_002520"
     CDS             674..1900
                     /locus_tag="IWO04_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0214084.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2058..2675)
                     /locus_tag="IWO04_002521"
     CDS             complement(2058..2675)
                     /locus_tag="IWO04_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214085.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(3358..4353)
                     /locus_tag="IWO04_002522"
     CDS             complement(3358..4353)
                     /locus_tag="IWO04_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0214086.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(4448..5320)
                     /locus_tag="IWO04_002523"
     CDS             complement(4448..5320)
                     /locus_tag="IWO04_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0214087.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(5333..6760)
                     /locus_tag="IWO04_002524"
     CDS             complement(5333..6760)
                     /locus_tag="IWO04_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0214088.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6814..8088)
                     /locus_tag="IWO04_002525"
     CDS             complement(6814..8088)
                     /locus_tag="IWO04_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0214089.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         2746 a         1524 c         1185 g         2836 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaatactt tagataaatg gattcgtata tataattttc ccgtaataaa aatagacggt
      541 ataattcgct ttgatgtaga agatatcaaa ggctttatgg atacgttcaa aaagatttga
      601 attttacttt tgactgcata tactaaaagt agcgaaatat actgccttac ttttttatta
      661 aggagtgagg aagttgccta cgaagctttc aaatggaaaa tataaaacta atttgagata
      721 tccaaaaaaa tttaaagaaa ttacaggtat cagttcagaa aaattccaaa aaacatttcc
      781 aactaggcaa ttggctatta aagccgaaaa tgaaatgaaa aagaaaatcg caaaagtctt
      841 aagagaggag aatgcaaatt cattagagct caaaggtcaa ataaaattca aggagtttta
      901 taaatctaaa tggcttccta gatatgagtt agggcaaact actagatccc ataaagctcc
      961 aagcgatata accatcatta atacaaagaa tttatttcga ttgcatattc tgcctatatt
     1021 tggtgagtat gccatgagct atcttaatgc aaataccgaa ataatatcag atgaactcac
     1081 aaaaaagtcg aaggaatatg caaacataaa aatcattaaa tgctatgttc gttccatgtt
     1141 tgatattgca gaggttctaa attatattga atttaatcgt acaacaaaaa taattcaaag
     1201 tattactgca cctaagaaag ttgctctgga agaaaaaaga atacgagaag gaaaacaagc
     1261 tttatcttcg aaagaactca ctgattggtt agacgctgta agagatgatt tcaatgatca
     1321 attactttcc tttcacgatt acactctttt tatgcttact ttatatcttg gggatagaaa
     1381 gagtgagacc tatgctttgc aatggaaata cattgatttt gaaaaccaaa cagtccgtct
     1441 taaatatgcc ttggataaac acacaaaaaa gaaatttaca aaaggtcgaa aagatactat
     1501 tatccaagtt ccggaaattg taatgacact attgcaagaa tggaaatcag tgcaagctga
     1561 acaactattg aagttgaaaa ttaaacagac acctgaccaa tatcttttta gttactccaa
     1621 accttctggt gaagtaaatt gtccagtcca tactgattac ttaaattatc gaattaattc
     1681 tataaaaaaa agacatcccc atttggttca tttaactcct cataaacttc gacatactta
     1741 cgcaacttta gctagacaag gtggggctga tatgaatcaa atttctaacg ctttgacaca
     1801 ttcaggtatt tcaactacaa aaatttatgt gaatacacct gatttagttg ataaagctgt
     1861 gttcgaagcg tttcagcgag gattacaaaa atctaattaa tgtgttctag aatgtgttct
     1921 aaatataaaa aaaagacact ttccaaataa ttggaaagcg tcttgaacgt tgatatataa
     1981 aggatattaa cgttttgaga attgagatgc tttacgagct ttcttaagac ctggtttctt
     2041 gcaaaattat attagcctca actgaaagat ttattttttt gctccctaac ttcattagta
     2101 ataccatgtt tcattcgata tcttctaaac gttctacgat taatccctgt tagctcttca
     2161 acttctttat ctgtagatcc agtcaaaaac aaattaaatg ctaattgaag tcgcggatca
     2221 ttctcacgat attttagcgt acctcccttg tatttccctt ttgctttagc gagtgcaatt
     2281 ccttgttgtt gcgtttcgcg aatataccga cgttcttctt cggcctggta cttgtatagt
     2341 tcgataatta agttattaag taatcgacgt aaattatcat cagtgatacc tgttaacgct
     2401 ggcaaattca aaacttccag tgtagctttc ttcagctgaa ttaaattcat cgtttctgtt
     2461 agctctttat tatttcgtcc aagtcgtttt aattctgtca caacgacaat atcaccttca
     2521 cgtatgaatt tcatcatttc ttgaaatcct tctcgattgg tatctttccc acttgcttta
     2581 tcactaaaaa ttttatcaca ctctgataat aattctaatt gtcggtctaa attttgatct
     2641 aagctgctta ctcgagcata cccaattttc gccataataa aactccttta aaattctgaa
     2701 atccggatca tttttaaatt ctgttgccca actctgttgt catttttcta gataaatggc
     2761 ataaattttc agcgctaagt gacaaaagca actaattttt catgatagat ttttaaatat
     2821 ctcaatctat ctccaaaatt agttgctttt gtagctatag ttgactatga atatgacaaa
     2881 taattagaaa aaactgtgcc aattaaatat aaaaatgaca ctcatttaca ttcaatagaa
     2941 caccaagagc ccaacagggt atactctggt gtccttttaa aaaagtaact ttctgaccta
     3001 attaattgag ctactaaaaa agctatgaaa tgaggaataa aaaagggatc aaaaactttt
     3061 aagtttctga cccttttgag ttctaattat ttcttccaga acgaagaatg ttatctgctt
     3121 tatctgcaat tgattctgtt agaaggactg aaaaatttat gccagccctt ttgcctagct
     3181 tatccgccca ttgaggaatt gttaaggttt ttctgacaag ttctatgtct ttcaaatatt
     3241 tggcaacatc tacacagatt aaaagcatga ttttgtatca tagtctatta taagagattt
     3301 aacaattttt tatatcacga attaataatg aaattatcat tgatagtctt tataaattta
     3361 attgttcttt ttctttactg atgtcttccc catccataat gtgacaggta aaatatattc
     3421 cttttttgtt tcttctttat ttattcgttt catcaaaata gcaacagctg tgtctgctat
     3481 atcattaatt ggttgtatta ctgttgtcag atctggtaat atactccgag tcattaaagt
     3541 accatcatat ccgactacta ataaatcagc tggtacattt aacccctttt cttttgctac
     3601 ttgtaatatt agggccgcat cgatgtcatt tgaagcaaaa ataccatcta ttttaggata
     3661 atcctcaaac atttggaaaa aaatctgttt tttctttaca taagaaatat tgaaatctaa
     3721 tgtcacagtt cgtggaatta actgatatgc tttcatagta tcttcatagg ctagtctccg
     3781 acgattagcc ggagtctgta agtcaattgg accgtttgta tgaataatat tttttgcacc
     3841 ttgtgcaatt aataacttgg ttgcaagttt tccaccattg tagttatcgg attcaacaac
     3901 tggaatatct tcattcatca ctcgatcaat tgcaacaatc ggtaaattct gatatttata
     3961 ttcctgaatt ccttggttat gtgtaccaac aattaaacca tctacttgat cagacaataa
     4021 ctgatttaaa taatttgttt ccttttcttt attattcatc gaattgccta ttaatacctt
     4081 ataaccaatt tcatataact ttttttccaa tgcctcaacg agttcactaa aaaaaggatt
     4141 ggctactgta ggaaatagta acccgacaat gttagttttc ttttgaaata attgacgcgc
     4201 tacaacgttc ggttgataat tgagttcttc tatagcctta taaacattat caatcgtttt
     4261 ttgacttaaa tagccacgat tgtttaatac acgtgaaaca gttgtttttg atacatttgc
     4321 tcttttggca acatcttcta attttgggcg cattctcttg tctctcctaa ataatgcaat
     4381 aattaactat cgaaaaaaag aactctatag aactactata cctgataatc gatatttttt
     4441 ctatgtttta ttcttgtaat tgattcaagc ccaaagcaaa gtttcctagt gttgccgatc
     4501 cattattttt aattaaagga cgcgtaatat atttacttaa cggtggtatt tccacgtaat
     4561 catccaataa tttattaaca ttaacacgaa ttttttctaa caatttttca tttactacgc
     4621 ttccaccaaa tactatttta tccggtcgta taatcaatgt tgtttgaatc acagcttgtg
     4681 ctagataata ggccattaca tcccaaatag gatccgttaa agaaacgtct tcaccctttt
     4741 ttccaaaacg tgcttcaaac gttggtcccg caactaagcc ttctaaacaa tcaccatgat
     4801 aaggacaaat tcctttaaaa tcaatatcat caggatggcg ttttacaaac gtatggccca
     4861 tttctgaatg accaaacgat cctaatagtt ttccgtcaac aacagctcca ccaccaacgc
     4921 cagtaccaac ggtgtaataa actaatgaat ttattttttc attcgataaa gtggacatga
     4981 tgtactctcc ataagctgat ccgttcacat ccgttgtcca agacattgga acattcagct
     5041 catcttgtaa ataacctaac acattaacat ttcgccatcc cacctttggt gtagacgtaa
     5101 tatatcctga ttttggtgaa ttttttcgaa tttctattgg accaaatgat gcaataccta
     5161 atgcatttat atcaaactgt ttaaaatagt caacagtttt tttcaatgtt tccctggggg
     5221 tggtcgttgg acaaattatt tggtcttgta ctcggtaatc ctcgtctccc acggcacata
     5281 caaatttagt cccacctgct tctatgctac caattaacat cataaaacct ccttactcgt
     5341 aagaaattgc atcttttttt agatgataaa ttgtactcga gacaaaagct tcttcactaa
     5401 cactcaaatg catacttggt tgattttctg tataataacg ctctgtaaaa acaagctcgc
     5461 catcattaat aaaaatctct gccgaacttt tatcgataaa cactcgaagt gataatctat
     5521 cacacatctg aatggttcca taccttggat catttttgcc gttacgagtt aaggtacatt
     5581 catttttatc cttagaataa aaaatattga ttaactcttc atcttttgtt tttaatgaaa
     5641 atttgacttc ttgatttttt gatttagtca atagtacatc aattagagct tctttgaatg
     5701 aagcatcttg agcgattatt atttctcctg ccaaattaac tgtcgtttca acgccgctgc
     5761 caactcgaag ttgttctaat tctctcacag gtttcatata caaacgatct ccaattaact
     5821 ctaattctcg aggaagcgtt aatgctcctg cccatccgtc tactttttca ggcatagggc
     5881 tttcccacat atccatccag gccataacaa tacgtctacc atctggagat aaagttgttt
     5941 gtgttgcata gaaatcgtgt cccttatcta attcataaaa atttcctcta atgaattttg
     6001 ctgtctcata atcataatca ccgataaagt agcctgtttc atatagattt aaataatctt
     6061 ttccatttgc ttcaattccc tgaggggata ataaaagaat agattttcca tttaattcaa
     6121 aaaagtctgg gcattcccac ataaatcctt ctgtaagtac ttccttcgct ttagaaattg
     6181 gtccaatgta atcccaattt aataaatcta acgactgata aacaatagca cgacctaatc
     6241 catctttttc ttgactaccc aaaaccatgt aatatttttc atcgtgtttc catacttttg
     6301 gatctctgaa atgatgtgtg ttatcttctg gtgcttttgc tattacagga tttttttcat
     6361 atttagtaaa gtgaatgcca tccgtactat acgccatgtt ttgattttgc caaaaatgat
     6421 ctggatcatt gtctccataa tagtgatgtc ctgtatagaa cagatacaac acaccatttt
     6481 tttcaatagc gcttcctgaa aaacaaccac cttcatcttc ttgatctcca ggagtcaaag
     6541 caagtggtaa agattcccaa tgaactaaat ccttacttct agcatgtgcc cagtgcattg
     6601 gtccccattc agcactatat ggatgatgtt gataaaacac atgataatac ccatcaaaat
     6661 agcaaaatcc atttggatca ttaatccatc ctgatggtgc agacacatga tatcccaaac
     6721 gataacgtgt atttgttaat tctatagctc tttttgtcat aatatattcg ctcactttct
     6781 agataaaatt gttttttatt ctgttggttt ccattatcct cgaataaatg gatccccttg
     6841 aacatctcta tcatcttgtt taagaataaa gaatgcatat atgcctgcta gtaatacaat
     6901 aagagaaata attttaaatg ttggttgata gccaacgttg tctcttaata ttcccaatgg
     6961 tgttgataaa atcacttgcc caatttgagc agctatctga aatcctatca tatagagggt
     7021 tgctgatagc tttgtatcaa aatgaagtgt aaaatagcga aacattggta atgtaaataa
     7081 tggtacttct aaagcatgca acatttttat gcatgaaaca gataatggtg tcgaggcaaa
     7141 cccgcacaat ccaattcgta cacacataat ggttacacct aataacaaag tatttcgaac
     7201 ccctaatttt ctcataatga ttggaacaat tcccatcatt aacgcttcac aaaatacttg
     7261 aatagcattc aatgtcccat atattttttc accattagct gatgttgaaa acaaaccagt
     7321 ataaaatccc ggaaacattt gttgatcgaa taccgtataa aatgtccatg taaaaacaat
     7381 aaagatgatt atcgtccata attgaggcag tttcattaga tcaaacattt cttttaaaga
     7441 aggaatacta tttgattcag cttgttcttg attaaaattt tgatttgctt cccgttcaac
     7501 tttagggttc caaaataata aatttaacaa tagtaaagaa ccaaagaaag atcccgccca
     7561 aaagtttaaa tgaggatttt ttacaaataa aaatcctgcc aaaagcgcta ccaaggcata
     7621 accaaatgat ccccacgccc ttgcttgtcc atattcaaag ccaaataaac gactaaacct
     7681 ttctgacaca gcttcaaaaa ctcctacaga agctaaatat ccagctgaca aaaatagcgc
     7741 tcccataata atgcctaaat taaaattgtt atctagcaat ggcccatata tccatataaa
     7801 aaatggtcca acaagtgtcg ctaatacaga agcaaaaatt aataaatttc gtttaatata
     7861 caatttatct tgtaatgttc cataaataaa catcaaaatt aaggtaacta acgaatttgc
     7921 cgagaatact gttcctacag cactgccaga taaccctaaa ccatttgatt cagatgttag
     7981 ccaaagttga aaaaatgacc accaaacacc ccaagaagca aaaaatagta ataatgtagc
     8041 tgaactttgt aaataagatt gatttttaaa tacgttaact tttgacatat ttttatcctc
     8101 caacgatata tttgtatccc ctttcatgat aaccggttaa catttttaga aaaggacttg
     8161 tactcctaaa attataaaag aaatccgctt acatgtcaac cggttattct atttggataa
     8221 tgccatataa tgtcacagga tatgactttt attggtgtgt cgcttttctt ttttaatggc
     8281 actatttttt a
//
LOCUS       DACECB010000091         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECB010000091 DACECB010000000
VERSION     DACECB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.093"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWO04_002526"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWO04_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0214090.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWO04_002527"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWO04_002527"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0214091.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWO04_002528"
     CDS             2591..3376
                     /locus_tag="IWO04_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0214092.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWO04_002529"
     CDS             3389..4240
                     /locus_tag="IWO04_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0214093.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWO04_002530"
     CDS             complement(4364..4816)
                     /locus_tag="IWO04_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214094.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWO04_002531"
     CDS             complement(5281..5649)
                     /locus_tag="IWO04_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0214095.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWO04_002532"
     CDS             5799..6281
                     /locus_tag="IWO04_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0214096.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWO04_002533"
     CDS             6318..7328
                     /locus_tag="IWO04_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0214097.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWO04_002534"
     CDS             complement(7443..8156)
                     /locus_tag="IWO04_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214098.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACECB010000092         8221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECB010000092 DACECB010000000
VERSION     DACECB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.094"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            150..1343
                     /locus_tag="IWO04_002535"
     CDS             150..1343
                     /locus_tag="IWO04_002535"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0214099.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1473..2945
                     /locus_tag="IWO04_002536"
     CDS             1473..2945
                     /locus_tag="IWO04_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0214100.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3028..3816
                     /locus_tag="IWO04_002537"
     CDS             3028..3816
                     /locus_tag="IWO04_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0214101.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3883..4551)
                     /locus_tag="IWO04_002538"
     CDS             complement(3883..4551)
                     /locus_tag="IWO04_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0214102.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4666..6378
                     /locus_tag="IWO04_002539"
     CDS             4666..6378
                     /locus_tag="IWO04_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0214103.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6381..8153
                     /locus_tag="IWO04_002540"
     CDS             6381..8153
                     /locus_tag="IWO04_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0214104.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2519 a         1380 c         1807 g         2515 t
ORIGIN      
        1 aaatattttt atatttttta agcaaaaaaa tgaaaaaata cgagcagaaa actgttttat
       61 ctgctatact tggttcgttg caagttcccg aaaggatccg caggacggag atgccttgta
      121 accgaattta actaggggga atcattataa tggtagaaag acacttattt acatcagaat
      181 ctgtttctga aggacatcca gataaaattg ctgaccaaat cagtgatgca atcttggatg
      241 caattttaaa acaagatcca acagcacgag tagcgtgtga aacgtctgta acaactggtc
      301 ttgttttagt atttggagaa atatctacga cggcatatgt agatattcaa aaagtcgtac
      361 gggaaacaat aaaagaaatt ggttatacac gtgcaaaatt cggatttgat ggagatacag
      421 cagctgtatt ggttgcgatt gatgaacaat ctcctgatat tgctcaagga gttgatgaag
      481 cacttgagat ccgggatgaa gataaaaaag acgtattaga tgagattggt gctggtgacc
      541 aaggtttaat gtttgggttt gctgtagatg aaacaccaga attgatgcct ttaccaatcg
      601 ctttgagtca tcgtctggta cgacgcttgg cagatttgcg taaatcaaat gaattaacgt
      661 atttacgtcc agatgcaaaa tctcaagtaa cggttgaata tgatgatcaa ggacaaccgg
      721 aacgcgtaga tacaatcgtt atttcaacac agcatgatga tgcagtagat aatgaaacaa
      781 ttcgtcatga tgtcatcgaa aaagtagtga aggaagttat tccagctgaa ttattagatg
      841 atcaaacgaa atattatatc aatccgactg gccgatttgt cattggtggt cctcaagggg
      901 atgccggatt aacaggaaga aaaatcattg ttgatacgta tggcggttat gctcgtcatg
      961 gtggcggtgc tttttctggt aaagatgcga caaaagttga ccgttctgcc agctatgctg
     1021 cacgttatat tgctaaaaac attgttgcag cagggcttgc tcgcaaagca gaagtacagc
     1081 tagcttatgc gattggtgtt gctcaacctg tttcgatctc aatcaatact ttcggtactg
     1141 gaacggttcc ggaagaagaa ctgattgctg cagtgagaga aaacttcgat cttcggccag
     1201 caggaatcat cgagatgctt gatttgcgtc gtccgattta caaacaaaca gctgcttacg
     1261 gtcattttgg tcgtacagat gtagatttgc cttgggaaca tacggacaaa gtagatgcgt
     1321 tgaaagcaag tttagcaaaa taagttatat tcagctggaa cgatcgccaa gtggtggttg
     1381 ttccagcttt ttttatggca gttaacctct tgaaaaagat agtaattatt tatctatttt
     1441 tagatcctgt cataatgaaa gggaggaata agatgaaaaa agaaacaaat gttactttgg
     1501 tcacagtgag tattttcatt gcaacgttca tgacggctat tgaaggtaca atcgtaacaa
     1561 ccgctatgcc aacgattgtc ggttcattac atggaatgga aatcatgaac tgggtatttt
     1621 cgatttattt gctgacaaac gctatgctta ctccgattta cggaaaactt gcggataaag
     1681 tgggacggaa accaatcttt atttttggga cattattgtt tattatcggt tcgttatttt
     1741 gcggtttatc tcaaagcatg attgggttga ttgctgctcg tgcgattcaa gggatgggtg
     1801 ctggggcaat gatgccagta gctttgacga tcatcggcga tctttattct attgaaaagc
     1861 gagcaaaagt tttaggtttg aatagttcag cctgggggat tgccagtgtt tttggaccgc
     1921 ttgcgggagg attcatcgtt gaaacgatca gctggcactg gatctttttt atcaatgtgc
     1981 cgatcggaat tctattaatt attttgattt ggctatattt aaatgaacca aaaatcatac
     2041 atgagtctaa accaatggat atttttggta gttttagttt gatggccatg cttgtctcat
     2101 tgctgttagg gttccaatta ttaggagacg aaggaatgag tccgcttgtt tctgctttgc
     2161 tgctaggtag tatcgtaatg ttaggtattt ttgtttttgc cgaaaaaaga gctaaagatc
     2221 ctgtcatcat gatgcattta ttcaaaaatc cactatttgt tgtggtcaac ttggctgcag
     2281 cattggctag tggttttttg atgggaatcg atgtttatat ccctatgtgg atgcaaggtg
     2341 tattagggct gaatgcagca attggtgggc tggtattagc accattatca ctagtctgga
     2401 tggtcggttc tttccttgca agcaagtgga tgactcaaat gaaaactcat tgggtcttga
     2461 gaattggact actaatcgca gctattgggg gcgcttggtt gtatcttatg cctttcagaa
     2521 gtaactattt atggtttttt gctatatcaa cggtcatcgg tataggctta ggtgtgacca
     2581 tgactacctc gactgtcagt gcacaaaata gtgtatctcg tgaccagctt ggtgtagcaa
     2641 cttcttttaa tacgttagtt cgaacaattg gtcaaacagt catggtttct atttttggtt
     2701 tattgataaa tggggtaact aaacgagaac ttgcagcagc aaatttaaca aatgatgcag
     2761 atatcatgaa taaactggtg aatccacaaa cggctaaata tattgatgcg gatattttgt
     2821 taccattaag aaaaattctt tatgatgggt tacattccgt ttatctagtt ggcttgttac
     2881 tcgtgatcat tgctttggtc ttaacgcttt tcgttcatga aaaaagtggt aaaataaaac
     2941 agtagtttcg gttaagatat gtacgtagcc gaatcattca aatttaaagt tattcaaaaa
     3001 taacttctta ctaaggaagg cgaaaatatg gcatttattc aagcaaatat ttattcgaat
     3061 gtgttagaaa tggaagtcaa tctaaatgtg atactaccgc aaacaacaaa aaaggtgatt
     3121 ggcaccagtt cagagcaaga gacaacggat atacctgttt tatatttact tcatggaatg
     3181 ggtggcaatc atagtgtatg ggaaagacgt acgtcgattg aacgttatgt agcaaactac
     3241 ggattagctg tcatcatgcc atctactgat cttggctggt acacagatac agtttatgac
     3301 atgaagtatt ggacattcat tgcagaagag ctacctgaga tttgtcatga attattcccg
     3361 caattaacaa gaaaaagaga aaaaacatat gccgctggat tatcgatggg cggatatggc
     3421 gcgttaaaac taggccttgc aaaatcagaa aatttcgcag cagtaatctc tctttcaggc
     3481 gctgtttctg taggaaatcg aatggatgat cttttgatgg ttcgtaaagc atctttttgg
     3541 gaagggatct ttggaccatt ggatcaaatc gaaggttcaa aaaatgatcc gcaatactta
     3601 ttgaaagaac tagtaaaaag cggaaaacca gttccaagat tttatatggc ttgtggggaa
     3661 tctgattttc tttatcaggc aaatcaggaa atggtatctg ctatgcagga acaaggcttg
     3721 gcggtcacct ttgaacaagg acctggcgat cataactgga tcttctggga ccaatggatt
     3781 caacgtgcat tggcgtggct aatggaagaa aaataaaaaa agagtcttaa aaagatagct
     3841 cctatagaga tcacaaacat agtgatctct ataggagctt ttttactgct cgttttctct
     3901 tcttttattt tgttggcata agccttcaat ataggtttgt gcactcgtta gaaagagtgc
     3961 tttttcattc aattctgcat gggactgcat actttcttta aatccctcta attgattttc
     4021 aactacataa ttacgcataa atagaactgt ttctttcaaa aattcatttc cgtccataat
     4081 tttttgtcga agctgctgtt catgctgctg atccattaac tgtccaatca ataaattgtg
     4141 gagagaggca atggcctgat gactttgggt gactgaaaat ccttcacgaa caagtaattg
     4201 gatcatttgc tccaaatgtt ctaatcgact cggaaaagcg ggcacagtat tgatttctaa
     4261 ttctgcccca caagggaaac gtgtataaag gtcatagtaa ttttccatat actgcagcaa
     4321 ctgttcattc caaggcaaag ttggatctgg aagcgctaat tcttcttcca ttgtttcagc
     4381 cattttttgc aagagctgtt gtttgttttt gaaataccaa tataaagcag gtgcttgaac
     4441 atgaattctg tttgcgatgt ttcgcatgga aagctgcgaa agagcttgtg tttcagccag
     4501 gatatcaaag gcggcctgaa tgatgattgc ttgagaaagc tttatttcca ttttatcaac
     4561 ctcgtgtatt gcattatctt tttaaacatg gtaaattgtt agtcggtcta attttacagt
     4621 gttaaattag tcctgtcaat ttaaacaaag gggtgtaatc aacgaatgaa attaatctta
     4681 cgacatgcga aaaaatataa agtagctgtt tttatttcct tgctgtccgt agcagtcatg
     4741 gttgcagcag cattatggca acctaagtta ctacagcaag tattggaagc tattattaca
     4801 gaagataatg atgaaatgaa aacaattggt atttacctga tcagtctggc tttgctagga
     4861 ttagccgcag gtgtaacgaa cacgattttc tctgcaaaag tagctcaagg cgtaagtgca
     4921 gatatccgag aagaagcatt tcgaaagatc cagacatttt catttggaaa tatcgaacag
     4981 ttctctgctg gaaatcttgt tgttcgtcta acaaatgata tcacgcaaat ccaaaattta
     5041 gtgatgattt ctttgcaatc actttttcga attccattct tatttattgg tgccttcatt
     5101 ttagctatga tcacgatgcc gcaattatgg tggatcatcg tagcgcttat cgttacggta
     5161 tttctgatta cggcactttc atttacaagg atgggcaagc acttcatgat cattcaaaaa
     5221 ctgattgatc gtgtgaatag cattgcaaaa gaaaacttac tagggattcg tgtagtgaaa
     5281 tcttttgtac aagaagaaaa ccaattgaag tcattcagca aagtaagtga ggacttaaca
     5341 aaacacaaca tcatagtcgg tacattattt tctgtgatga tcccgtcctt catgctggct
     5401 gccaatttgg ctgtggttgg ggcgatcttt tttgtgagcg atcttgttaa agatgatcct
     5461 acgttaattg gtggaattgc ttcattcatg aactacttga tgcagattat gatggcaatc
     5521 attattggcg gaatgatgat gatgatgact tcaagagcag ccgtatcgct gaagcggatt
     5581 gcggaaattt tagatgctga accagatctt acttatttag acgttccaga acaagagttg
     5641 acgggttctg tcacttttga ccatgtttct tttcgttatc ctggtgagga tacagatacg
     5701 cttaaagata tcagcttttc tatcaaacct ggagaaatgg ttggtatcgt cggggcgact
     5761 ggtgccggga agtcaacact agcacaattg atccctcgtc tatttgatcc gacagaaggc
     5821 aaagttgaag taggtggtgt agatcttcgt caagtaaatg aaaagagttt aagacaaacg
     5881 gtttcctttg ttttgcaaaa agcaatttta ttctcaggaa cgattgctca aaacttgcgc
     5941 caagggaaaa agaatgcaac agaaaaagag atggaacatg cttcttctat tgctcaagca
     6001 aaagaattca tcgaaaagtt atctgatcgt tacgaagcac caatagaaga acgtagcagt
     6061 aacttttctg gtggtcaaaa gcaacgttta tccattactc gtggcgttat cggtaatcca
     6121 aagatcttga tcctagatga cagtacgagt gcactggatg ctcgctctga aaagctggtc
     6181 cgagaagcac tcgatcgaga tttgaaggga acaacgacga ttgtcatcgc acaaaaaatt
     6241 gcttctgttg tcaaagcaga ccgcatctta gtattggatg aaggcagatt agtcggcgaa
     6301 ggaacccatg aggaattggt agctggaaac cgtgtctatc aggaaatctt tgaaactcaa
     6361 aaaggaacgg aggagtaagt atgactgatc taataaaagc aagtaaattt ttctatcact
     6421 atttgaagcg gtacaaactc tcctttttct ttatctttat cacagtgatc attgctacct
     6481 atctacaagt aaaggcacct caatacatcg gggaagcttt tcaagaatta gctaattacg
     6541 taggaggctt gatgcaaggc gtagacgata aaagcaaatt tgtttctgta atatggaaac
     6601 tcttgctgtt ttatgtacta gctagtgcag ccaactttat ctatagtatt ttgttcacac
     6661 aagtagtagg gaagtcaacg aaccgaatgc gtatcggtct attcaacaaa ttggaaaaat
     6721 tgacgatccg tttctttgat tctcatcaag acggtgaaat tttaagtcgt tttaccagtg
     6781 acttagataa tattcaaaat agtttgaacc aagctttgct tcaagtcatc acgaatgctg
     6841 ttttacttgt cgggatcttg atcatgatgt tccgccaaaa tgtagagtta gcttgggcta
     6901 caattgcttc cactccagta gccgttttga tagctgtctt gattatccgc aaagcacgga
     6961 aatatgtgga tcttcagcaa gatgaagtgg ggaaactaaa tggttatatg gatgaaaaaa
     7021 tcagcggaca acgtgtcatc atcacaaatg gcttacaaga agaaacgatt gaaggattct
     7081 tagagcataa tcaaagtgtt cgcgaagcaa ccttcaaagg acaagcttat tctggtctac
     7141 tatttccgat gatgcaagga atgtcactgg tcaatacagc gatagttatc tttttcggcg
     7201 gctggttagc attaaatggt gatttggaac gttcggtggc attaggctta gttgtgacat
     7261 tcgtccagta ttcacaacaa tattaccaac cactgatgca gatttcatct ggatacagca
     7321 tgatccaatt agcgattaca ggggcaagac gattgaatga aatatttgat gaaaaagatg
     7381 aagtcaaacc agaaaacggt aaagcatttg atggtgttga aaaaggtctt gcgttgcagc
     7441 acgtagattt tggttattct ccagaaaaac tagtcttgaa agatgtctcg atcaatgtgg
     7501 acaaaggaga aatggttgct cttgttggtc caacaggatc ggggaaaacg acgatcatga
     7561 atctgctaaa ccgtttttac gatgtcaatg atggttcagt agtcatcgat ggaatagata
     7621 tccgagatta tgatcttgat gcattacgta gacatgtagg aatcgttttg caagaatctg
     7681 tattgttctc cggaacgatc aaagacaata tcacattcgg caaaccggac gcttcagatg
     7741 aagaagtcat caatgcagca aaacaagcga atattcatga cttcatcatg acgttagaaa
     7801 aaggttatga tacagagata tccgaagaaa ataacttatt cagtacaggt caaaagcagt
     7861 tgatcagtat tgctcgaacg atcatcacaa atccatcttt attgattttg gatgaagcaa
     7921 caagtaatgt agacacggta acagaagcaa ggatccaaaa agcaatggat gaagcaatca
     7981 aaggaagaac cagctttgtg attgctcacc ggctgaaaac aatcttgaat gcagatagaa
     8041 tcgttgtctt gcgtgatggg gaagtcattg aagaaggcag tcatcatgag ttattagcag
     8101 aaaatggatt ttatgcggaa ctttatcata atcaatttgt atttgaaagt taaattaaaa
     8161 gaacctcgtt ataacggggt tctttttata tattttatgt tgtttgtttt tataaggggc
     8221 a
//
LOCUS       DACECB010000093         8066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECB010000093 DACECB010000000
VERSION     DACECB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.095"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IWO04_002541"
     CDS             <1..883
                     /locus_tag="IWO04_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0214105.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IWO04_002542"
     CDS             931..3060
                     /locus_tag="IWO04_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0214106.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IWO04_002543"
     CDS             3035..3724
                     /locus_tag="IWO04_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214107.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4817)
                     /locus_tag="IWO04_002544"
                     /pseudo
     CDS             complement(4132..4817)
                     /locus_tag="IWO04_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4953..5147)
                     /locus_tag="IWO04_002545"
     CDS             complement(4953..5147)
                     /locus_tag="IWO04_002545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214108.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IWO04_002546"
     CDS             complement(5197..5637)
                     /locus_tag="IWO04_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0214109.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IWO04_002547"
     CDS             complement(5641..6486)
                     /locus_tag="IWO04_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0214110.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="IWO04_002548"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="IWO04_002548"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0214111.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DACECB010000094         7765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECB010000094 DACECB010000000
VERSION     DACECB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.096"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..185
                     /locus_tag="IWO04_002549"
     CDS             <1..185
                     /locus_tag="IWO04_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ0214112.1"
                     /translation="CKFTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAV
                     VKIGEKGYIDFISFSK"
     gene            319..588
                     /locus_tag="IWO04_002550"
     CDS             319..588
                     /locus_tag="IWO04_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0214113.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVQYLKILCIIGIEVKLDAKNL"
     gene            609..692
                     /locus_tag="IWO04_002551"
     CDS             609..692
                     /locus_tag="IWO04_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0214114.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            817..1554
                     /gene="erm(B)"
                     /locus_tag="IWO04_002552"
     CDS             817..1554
                     /gene="erm(B)"
                     /locus_tag="IWO04_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0214115.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1724..1984
                     /locus_tag="IWO04_002553"
                     /pseudo
     CDS             1724..1984
                     /locus_tag="IWO04_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            2147..2812
                     /locus_tag="IWO04_002554"
                     /pseudo
     CDS             2147..2812
                     /locus_tag="IWO04_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2848..3093
                     /locus_tag="IWO04_002555"
     CDS             2848..3093
                     /locus_tag="IWO04_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0214116.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            3196..4065
                     /locus_tag="IWO04_002556"
     CDS             3196..4065
                     /locus_tag="IWO04_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0214117.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            4046..4780
                     /locus_tag="IWO04_002557"
     CDS             4046..4780
                     /locus_tag="IWO04_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0214118.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4813..5721
                     /locus_tag="IWO04_002558"
     CDS             4813..5721
                     /locus_tag="IWO04_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0214119.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5718..6260
                     /gene="sat4"
                     /locus_tag="IWO04_002559"
     CDS             5718..6260
                     /gene="sat4"
                     /locus_tag="IWO04_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0214120.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            6353..7147
                     /locus_tag="IWO04_002560"
                     /note="aph(3')-IIIa"
     CDS             6353..7147
                     /locus_tag="IWO04_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0214121.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(7255..7443)
                     /locus_tag="IWO04_002561"
                     /pseudo
     CDS             complement(7255..7443)
                     /locus_tag="IWO04_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7423..>7765
                     /locus_tag="IWO04_002562"
     CDS             7423..>7765
                     /locus_tag="IWO04_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0214122.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEK"
BASE COUNT         2763 a         1156 c         1617 g         2229 t
ORIGIN      
        1 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
       61 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
      121 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttata tctttctcaa
      181 aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat cacttatcac
      241 aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat aagaataaga
      301 agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga aaaacgaaat
      361 gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg tgttcgtgct
      421 gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt
      481 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
      541 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
      601 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      661 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      721 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      781 tttatttaca gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      841 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      901 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      961 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
     1021 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
     1081 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
     1141 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
     1201 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
     1261 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
     1321 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
     1381 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
     1441 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
     1501 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1561 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1621 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1681 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1741 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1801 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1861 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1921 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1981 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     2041 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     2101 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     2161 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     2221 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     2281 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     2341 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     2401 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     2461 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     2521 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2581 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2641 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2701 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2761 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2821 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2881 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2941 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     3001 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     3061 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     3121 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     3181 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     3241 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     3301 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     3361 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     3421 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     3481 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3541 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3601 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3661 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3721 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3781 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3841 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3901 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3961 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     4021 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     4081 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     4141 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     4201 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     4261 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     4321 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     4381 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     4441 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     4501 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4561 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4621 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4681 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4741 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4801 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4861 caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct
     4921 aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata
     4981 tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat
     5041 atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac
     5101 aataaaattg atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat
     5161 aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact
     5221 gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa
     5281 ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct
     5341 attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc
     5401 ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat
     5461 atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac
     5521 atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg
     5581 gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata taccagggac
     5641 atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata
     5701 gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata
     5761 aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt
     5821 ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg
     5881 atgaagagga tgaggaggca gattgccttg aatatattga caatactgat aagataatat
     5941 atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc
     6001 ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca
     6061 gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc
     6121 ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa
     6181 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
     6241 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
     6301 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
     6361 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
     6421 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
     6481 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
     6541 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
     6601 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
     6661 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
     6721 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
     6781 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
     6841 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
     6901 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
     6961 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
     7021 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
     7081 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     7141 gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta
     7201 atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa
     7261 gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag
     7321 tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca
     7381 taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga
     7441 gtaacctcta ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca
     7501 cagcgattta atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc
     7561 ggaataccaa ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt
     7621 ccgccggtaa tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta
     7681 aattgccatg gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa
     7741 tctattttga agcatggtga aaaaa
//
LOCUS       DACECB010000095         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECB010000095 DACECB010000000
VERSION     DACECB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.097"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWO04_002563"
     CDS             complement(138..428)
                     /locus_tag="IWO04_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214123.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWO04_002564"
     CDS             complement(546..806)
                     /locus_tag="IWO04_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214124.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWO04_002565"
     CDS             complement(943..1356)
                     /locus_tag="IWO04_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0214125.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWO04_002566"
     CDS             complement(1812..2297)
                     /locus_tag="IWO04_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214126.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWO04_002567"
     CDS             complement(2436..2651)
                     /locus_tag="IWO04_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0214127.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWO04_002568"
     CDS             2875..3675
                     /locus_tag="IWO04_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0214128.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWO04_002569"
     CDS             3694..5562
                     /locus_tag="IWO04_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0214129.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWO04_002570"
     CDS             5555..6046
                     /locus_tag="IWO04_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0214130.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWO04_002571"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWO04_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0214131.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWO04_002572"
     CDS             6606..>7600
                     /locus_tag="IWO04_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0214132.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACECB010000096         7403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECB010000096 DACECB010000000
VERSION     DACECB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.098"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IWO04_002573"
     CDS             complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IWO04_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0214133.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
     gene            complement(2939..3244)
                     /locus_tag="IWO04_002574"
     CDS             complement(2939..3244)
                     /locus_tag="IWO04_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214134.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3389..4594)
                     /locus_tag="IWO04_002575"
     CDS             complement(3389..4594)
                     /locus_tag="IWO04_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0214135.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5288..5674
                     /locus_tag="IWO04_002576"
     CDS             5288..5674
                     /locus_tag="IWO04_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0214136.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5772..5963
                     /locus_tag="IWO04_002577"
     CDS             5772..5963
                     /locus_tag="IWO04_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214137.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6477..6809
                     /locus_tag="IWO04_002578"
     CDS             6477..6809
                     /locus_tag="IWO04_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0214138.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6719..6922
                     /locus_tag="IWO04_002579"
     CDS             6719..6922
                     /locus_tag="IWO04_002579"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0214139.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7106..7261
                     /locus_tag="IWO04_002580"
     CDS             7106..7261
                     /locus_tag="IWO04_002580"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0214140.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2472 a         1307 c         1061 g         2563 t
ORIGIN      
        1 ggtgaaggtc actttgtggt agcctgcagg aatgttttct cctggatttt cataaatatc
       61 tttacttaca gttactgcta aatcggtcgt gcctacagaa ccatcttggt aaacaatttt
      121 aataggaatt gttccagtaa tcggctccga ttctcctaca cctttagctg gagtgaatgt
      181 aacaatacca gtatttgcat caacacttgc attaccgaag tccttattat agacatctaa
      241 aattgaaaag gcatctttag ctaaaggaac atctggttta tcaaatgtag gttttccact
      301 ttgaactgtc gtttctggag ttgttgttat aggtacgtat gttgcatcat tttccatagc
      361 ttgagaagct tttacagcaa actctacatc cacgtcgaaa gttcgatttc cgatcttaac
      421 agttacaata cctttcgtac tatcagtaac gttagaagta tcaggctctg ttttccatga
      481 atactctgca tcttcaggta agtttttatt attccgaatt gattctttag catctggttt
      541 aaatccaact accacggttt gtttatcaac cgcttttggt gattccttaa taacggttga
      601 accttcttct ggtttatcaa aacctggaga aacgatttgg atttttaatt catctttcgc
      661 gattaattta cttgaatcta cacccgtaaa ttcaaattct acagtatctg aaattggagc
      721 cccatctttt tcatttggag cgatagtttt ttctgctaat acagtacctt ctttatcaac
      781 aagttgggct ttgtgacctg ttcccgctaa ctcgtggatg aatactttcc ccttaactgt
      841 tgtgtcaaca tcatatactt cttctaattc aggtttcccg atatatggat tcgtgtctcc
      901 gctctcttct ttatcgtcag ggtttccatc accatcaata tcttgcaaat aataataacc
      961 tgttccataa gtgttctgga ttaatttttt attcgtatcc gacaaccaag aatcaaagat
     1021 gaaatcttca ccaaatggtt tattttcttc aacttgtgct gttgctttca tatcttctaa
     1081 gatttcatct aatggcttag tcaattgtaa aacatatcga ataacgactg gacgtggact
     1141 tgcattagca aaatcaataa gtttggtaag atcattatca gtaattgaac ctacaccata
     1201 attttcatga tctttgcgcc gttcaattgc taaatcagcc acttttttcc ctgattcaaa
     1261 agaaacatca tatgttactt tgtcagcctg aaccatgtgc atctgtatac ccgctacata
     1321 tggaactaat cgttcatcaa ttttgtaatt agcataccaa ggcgtgccct ttgcggaagc
     1381 tgaatatgag aaattataat ttttcgttaa cttatgatct aagattatag ctccattagc
     1441 acctgtggat cctgaaccat cttttgtttg agtattaggt tcctcatatt ttgcacttac
     1501 tgatccatgt ttaaactggt caggattatt ttcagataca tctaagctat tatagaaatc
     1561 atctgaatct gttaaaaagt agcccgtact atcaatacct ggtacaattt tatcatcgtc
     1621 ttgatgactt accaagtaga ttcgatacat taaacgatcg gtcttgagat tgtctatgcc
     1681 tatctcttta aaaatttcat ctaagctctt ttcaaacgta attaccccat taggagcagt
     1741 ttgtgtatca gttgtttcag ccccagcaaa taatccatca cttgcacgaa tataattgac
     1801 cttccaaata tttgtaggtc tacccagcga atcattaatt cttacaaagg tccgtctaga
     1861 agatccaatt cctttctttg gatcgacctg aattttttct acatatttag ctaatcgttc
     1921 atctaattga agacggattt cccagttttt agttgatgct gcaatattat gttttccgta
     1981 aacttcaaat ccaattgtat tgccttttag tgtgtctcca gttagttctt gaggactaaa
     2041 ttgtaaatcc gaaaagttat aatttttcgt ataaccagga ttctcctgaa aagtattccc
     2101 cgaagctact ttgtcaccta cttgtacgga gcttatcgct gtttcaagtg gggtgcttgg
     2161 agaaatttca tcttgtgctt gaactagttc agttgttccc aagacaagac caacaccaac
     2221 caaaacagat gcaaccccaa cacttaattt tttgatactg tacttcaata ctcgtttctt
     2281 tgttttttct agaaatactc tcttattatt cttgctaacc attattcaaa tcaactccct
     2341 taatagttat tttcttagta agaataaaag ataaatctga tacgtattat tcacagaata
     2401 ggaatcttac taaacttatg ttaactagtt tacttaaaga atttcatgca cattacaaca
     2461 aaaaaagtac atttcttcat gtttttttcg gatatttgtg ctttttaacc attcagcagc
     2521 tgaaaacata taaaaagata tgtatgtatt ctttctgaaa ttaagttcat ttctatgcga
     2581 tgaagcatgt tttcaaaata ttactctttc ttagattaaa cttcagagta ttcaaatagc
     2641 ggaataagga gccttatccc gcattactga atgagactgt gacataagta aaaaggtgac
     2701 ttcaaattcc ttcttcggaa tttgaaatca ccttctttat aattataaag tatcggtttc
     2761 acagcattcc tgaatgattc ctagaatctt aaacgaatga ttggagttat caacattttt
     2821 tcactcgttt ttagcgaaac aaaaaatgac atttgtcaca gcctcattaa attattgatt
     2881 ctttttcttt ataattgaat tgtccatatt taaagtgtct agtactctac atgaataatt
     2941 aattgctttc actcaaatac ttataatcta ttaacttgta gttgtttaac ttttctgaaa
     3001 aagttagacc cacgtaataa tcttcccagc tttctctttc aattaactga tccaattttt
     3061 gataatccat aaattcatca tgtattaaaa agaataaata acttgatatt ttagcatcta
     3121 gcttaatcag attaaataat gtttgcttaa aattgctacc attaatatga ctgatagatt
     3181 ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca tattcattta
     3241 acacactatt attaccatta cttagacgct cagattctac gaaacaactc aaatcagaaa
     3301 tttcccaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac tacgtaaatg
     3361 atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaatta tccactaaac
     3421 ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc gaaaagtgtg
     3481 aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt gatttcaaca
     3541 ttattttagc cgtagaaatt tttcgcttag agatataatt ataaatcgta atatgcattt
     3601 cattcttaaa gctttctctt aatttagttt cacttacaga aaaataatta gctatttcct
     3661 ttacagtaat tcttttatag atatttaagc cgatgaattg aacgacagaa gaaattaagg
     3721 gagaaagatg ctctatctta gtttttccga tttccttagt ataataaaca atcccagaat
     3781 ccctcacttt taagacctca ggtaacgata cagctaattc gttttcttgt atcaaagcat
     3841 ctctactttg atacgcttct attatatcca ttcctaatgt tatagacgtt tgtgctaact
     3901 tttcaaaaac aatgatagaa taattttttt cagaccgaat cgtatctcca ctaacacttg
     3961 gaattattcc attactcaga ttaaaaacca tgttttctaa atttcgaata tctccatttt
     4021 taacatattc cagtatttta ttttcataaa gaaacagata tatttctgga tcgtaatttt
     4081 gtgataacag attatctatt ttgatttgct cgatagtttt acttagattt cctgtatatt
     4141 caattaacgg ttcatggaat agatcttcta tttttccagt aaaaaaataa tgaactaaaa
     4201 tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa attttgcaat gaattataga
     4261 gtcgttctct atcagccatt gcaacattca aattgttcat ttttagttta aaaaaatctt
     4321 tatcaatatt atttgctcta aatggaccaa ataagtaata tctggtttta aacatgtata
     4381 gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa atctttagtt tttttaatat
     4441 tatcagttag taacttttta taatcataaa aaaaataagt agttctatct gagcgatact
     4501 cttttataat actgaattcg gaattaaaaa ccacgatagg taactttaaa caaacatgaa
     4561 gagactttaa taagacttct ttatctgaaa acacttttgt ttctctcttt cctttctaca
     4621 tcattaaata gtattttata cgttatctaa aatactattc ctctgtcaaa ataagctaac
     4681 ttacttcatt ataaaatatc cacttatcgc tttcatgtat attccaccgt aaaaagtaca
     4741 ttttatcacc gagaactcat catttttcat catgatgaat actatttaca tatatctagt
     4801 tcaaagacaa taattattat gatccgttat tatctaagca ctatacagat aataggaatt
     4861 tttcgtttct aaatactcca atttgggaat ccaataaaaa ttttcgttca aatacgtgtc
     4921 tattcgaact cttttctagt tgttaattat gaagggggaa gaaataaaaa atacaatcta
     4981 tacacgaata agaatattat ccaaaaggag gttttagaaa agatttatta tacgtaaaaa
     5041 gctcaagaca ttttcttagc cataaggaca tgtttgatat ttaagaagat atatgaattt
     5101 tcttttctaa gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa
     5161 acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat
     5221 tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt
     5281 gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa
     5341 ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga
     5401 acaacttgat caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc
     5461 gattaatgtt ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc
     5521 tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa tttatcttta ataaaactga
     5581 taaagaaatt ggaaaggttc tgggtattac aagacagggt gcgacaaatt taaaacatcg
     5641 actttacaaa aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat
     5701 gcttctttgc tcgtcctcct ccttcccaaa aaaataaata gattataagt tcatgggaag
     5761 aaggtaaatt tatgaatact ttaaaagaag atttcatttt agcaaagaat ggaaatgaag
     5821 aagcagttgt ggccattctc aaaagattta gcgctttggt ccataagcaa tcctggagaa
     5881 ctggaaaata tgatcaagat tgttatcaag aatgtatgct tgcaatatat ctagctatct
     5941 cgaaattcga aattaaagaa taactcaact aagggagtct gtgacataag taaaaagata
     6001 gtttcaaatt tcgaacaata aatttgaagc catcttcttt ataattataa agtatcgatt
     6061 ttacagcgtt ctataatgat tcatagaatc taaaacgaat gattttaatt attaacgttt
     6121 tttaattcgt ttttagctta aaaataactt ttgtcacagt ctcactccta aaattacttt
     6181 atttacgtac gaagtgggca ggaggtcgaa tccagaactg ggccatggta cttcaacgag
     6241 gacttctcct ctagaattga aaagcatgcg atttaccttc cgtaacgacg gtagatttat
     6301 tcaactatgt gtccaatcaa aatttgtctg atttcaaaaa tagtcaagag aattttggtt
     6361 gttaaaaact cttatctatc aacttccaca gtttagttga caaaccctga tactaagttg
     6421 ggctatgata cacttttctt gactatcagt tacgttaccg gaaggaatga actctattga
     6481 aaaaaacata tgatcgtaaa tttaaactaa agatttcaca ggatatcctt gagaaaaaag
     6541 taactaccaa gaaaattgct gaagaataca atatctctcg tccaacaatt tcccgatggg
     6601 tttcagaata tcgtcgatac gggaaaaatg cttttgctgg acaaggaaaa agattacctg
     6661 ataaagcaga tttctacatt cttgaacagg aaaataaacg actaaaagag gaagacgagt
     6721 gttctacgaa cacaaagatc gatacggatc tccagggatc actcaaaaac tttacgatga
     6781 aggaatcgaa atcaataaac gtgttgtagc tgttcttatg cgtgaaatga atttatgcac
     6841 aagaggattt catcgacgca aatcttctca tggtagagaa aaaacaatca aagaaatggt
     6901 aaaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt gggtgacgga
     6961 tattacttac attccatgta atgacggtcg cctctatttg tccacctata ttgacttagc
     7021 aactcgtatt ctacgttact atatggtgga ttcacacatg aaaaaagaga ttgtcgtccg
     7081 tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata gaggtagcca
     7141 gcatcggtct tttatttatc aagagctgtt gaccgaccac cacatcacac attcaatgag
     7201 ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat caatcaaatg
     7261 agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt taatccaaga
     7321 ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga tgactccttg
     7381 acaatttgaa gcgaagaaaa taa
//
LOCUS       DACECB010000097         7302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECB010000097 DACECB010000000
VERSION     DACECB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.099"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(35..2146)
                     /locus_tag="IWO04_002581"
     CDS             complement(35..2146)
                     /locus_tag="IWO04_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0214141.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(2435..3259)
                     /locus_tag="IWO04_002582"
     CDS             complement(2435..3259)
                     /locus_tag="IWO04_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214142.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3256..4011)
                     /locus_tag="IWO04_002583"
     CDS             complement(3256..4011)
                     /locus_tag="IWO04_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0214143.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4024..6000)
                     /locus_tag="IWO04_002584"
     CDS             complement(4024..6000)
                     /locus_tag="IWO04_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0214144.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6053..6724)
                     /locus_tag="IWO04_002585"
     CDS             complement(6053..6724)
                     /locus_tag="IWO04_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0214145.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6784..6993)
                     /locus_tag="IWO04_002586"
     CDS             complement(6784..6993)
                     /locus_tag="IWO04_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214146.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7060..7299)
                     /locus_tag="IWO04_002587"
     CDS             complement(7060..7299)
                     /locus_tag="IWO04_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214147.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2011 a         1496 c         1101 g         2694 t
ORIGIN      
        1 aaaacaaggc tgattctcag ccttgttttt atatttattt gagttttaaa actacatagc
       61 ccaatgatgc taaagtaaga aataatatac ttccaataac agttagcact aaatgattcg
      121 atccagtttt tggcacaatg atctgaggta agttaggttt taccccaatc gttactacat
      181 tagttgcttt ctcaatactt tctactgaaa cttttgcttg gttgtcgata tccaattctt
      241 cattcaaagg agttttagct gtaaatatca gctgaatttc tcgtccatct aaaagatttt
      301 tacttaagaa atcagcggaa gcttcccact tcagcgttct tgaattgcta tcataggtta
      361 gtgtaccttc tgaagtaata tcttttcctg tactcatatc tatcactcgc gcagaacctt
      421 ctttatacgt caaacgatct tccactggat cttgaatact aaattgcttg taatgatctg
      481 aggcaatgtc tacacctttt gtatgccatt tttgtgtgac tagatattgt acttcagatc
      541 ctgcatttaa aggagctgta ggcgttcctt ttttaccatc atttcgacca tcaacatctt
      601 tcccatcttg ttccactttt ttatttggtt taagagagaa atagttttct actgtatttg
      661 ttttttgtgt tcctttgttc ggatcattgt taaacgttac agtagccaca tttggtgcaa
      721 ttccttttgc atcatttttt gctctcatat ggagctgcaa aatataatcc ctaccgtaat
      781 ttgcagcgtt agctaaaaag ctgtcagaag cagtataagt gactgtatta cctgatacag
      841 ttaactttcc tcctgtatca gcttttttcc aagtaccatt cgcttcaaca taccacaatg
      901 cgccatcata gtttttccca tccgatacca tatcaaaatt tttgctgtca aaggtatcag
      961 tgattttaaa tgatttgtat ttgttcattg tgttgactgt taatgcatca aacttttggt
     1021 gaatatgata gtaaacatct gatccttgtg gaatcacttt tttgtcaata ttgtgtgctg
     1081 cctcttcagt attagctttg ttaaacactt cttttgtagg cttcgatggt tgtggtgtcc
     1141 atagcaatgc agtatcaaag ctaaacatca tactattcgt atttgcgcca atcatgaatg
     1201 ttggggaagc gccttgaagc gtatacgtta caccacttct gtgataagta tcagctccta
     1261 caacatcggt gaaatgatca ttcgagctat aagccttttc tgcacgtcct cccactactt
     1321 ttgaagttaa attgtttgtt gataacgcag gagctttgta ttctgcacat gctgtatctg
     1381 ttgtgacaaa ataaggtaag ctacccattt tttcataccc aacaaattct ctcatatcaa
     1441 tattacgttc accgacagat tcattattgt cattatacca agacacacgt tgctgatact
     1501 gtccatttaa actgtaaatg gatagaaatg tatcgtttaa atcaatacca gctgctctca
     1561 tcgatctcat ttttgattga atttctggct gtggttcact caaagctact tttgtttttg
     1621 ttcctgcata cttaaattct agttttgcat tcatgacatt gattgactgc atgaaaaatc
     1681 ctcgataaat atttttatca aagagcaaat aattataata ctttttgcca ttaattgttt
     1741 tagctaatcc gtcttggtta accccagcgt gcgctacaaa attagaaaaa gtcacgtcac
     1801 aatcaatttc ttttcctaga taagtaccta ctttttgata atgggcaacg atttttgacg
     1861 tacttttcaa atctttgact tttggccaag caagtacttt ttgtttttta gttgaatact
     1921 catctagtat cttaaatcca ttgatcccag ttacttcgat atttctcaat gaatcactat
     1981 catgataaag caccagatta ttattttctt tgatttgact cggatcaact ttttctaagg
     2041 cctggactgc agttcccctc gcaaaaagaa gaaggaacaa aaacaacact aaagtaatta
     2101 ctcttcccgt agtgcaatga attttatttt ccataatgcc ctccatctaa tattttttta
     2161 aacgatcgta agcttttcat tctttttttc ctaataccca cgttaccaat cctgtaagaa
     2221 gaaccaaact aaccataaaa attggattga tcaagagctt tttaatcacc tctaatggca
     2281 tggaagcaca ttgaatgttt agaaaatgaa tgaacaatcg ttgatatctt ggataaattg
     2341 ataattgaga aaaaattaaa ccgcaacaca atattgttgc ggctaaaaag gatagattaa
     2401 aaactaatat tcttattatt ttcatcattt catcctaatt atcaaaaaat aattgagcaa
     2461 aaaattccca acaattttca aaaaagttct tctttacacc agttgacaca acttttttaa
     2521 tttcttcttt cggctctgat ttctctgatt tagggtataa ccaaacatcc gtcaaaaatt
     2581 cacctttttc atttgaaaca ggtgtcaata aaacaatatc ttcactgttt gaaatgtttt
     2641 cagggcttga ttcattaacg atatagtatg cttgtttttt gtttgcaggt acttcaatgg
     2701 aaaattcaat cacgccttcc tgatcatcaa tcgttttggt ttctccttca gccacttttt
     2761 tcaactggtc gtatgaaagt ttttttgctc gatctttcaa ttttgactca attatttcta
     2821 cagattgatt tttctcatta tcatcttgtg acatttcttc taagatattc gtgatatcgt
     2881 agacaataaa cttcgcgcca ttgatacctt cttgctgggc tacttgactg ccttgattgt
     2941 tgatttgatt cgaagaaata attttttgaa tatgaatggc ttgcttgatc acttgcgtat
     3001 cttctacgtc ttgcggcttt tctgaagtct ctgaagaact ttctgacgtt ggattcgttg
     3061 attcagtaaa gcttatattt gaatctgccg tttcttctgt agtattctcc gaactgtccg
     3121 tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt ggtactacta tcactgctgt
     3181 cagcgaaaga cagttgcgaa taagctacag tagaaaagag agctgctaca caaacaaaca
     3241 gccattttct cttattcatt ccgatctcct ccttattttt ttataaataa aaataatagg
     3301 aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc aatgaaattg atttcctatt
     3361 ttttgctttt ttctgtagtt ttaaatcttc tttactgaag gaaactcgtt caccatacac
     3421 taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc attaacgtga tcctatcttg
     3481 gtctttttca ggcataagat aatctaaata atttttttgt aattcttctg ctttaacttc
     3541 ttcaattcga taaatttgat aggcatgttg ttcttcattt accttaataa agaatttttg
     3601 cccttttttt actgaaggca aatcatcgaa aagattatct ttataaagtc cactgtgccc
     3661 cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg ccagttcctt ccgtaatccc
     3721 tacaccattc tctaaaattt tgtcggaagt tccgtcataa attggtagtt ttaaagaaat
     3781 acttggaatt gtaagaacac caacaggatc gcctaactct attttcgcaa catccaaaga
     3841 agaatctgtt ttttcaagat tttcaatggc taaaattctt tgctgttgtt gaaatttcgc
     3901 tacatttgtt tccacaactg gaattaaata gactgctact gccgacagca accaaaaaaa
     3961 tatcatcagt ttgtgcatga ctcttctttt tgggggctta cgaataatca ttagctgatc
     4021 cttctaggct ttcttcttaa agtaaacaac tgcagaaagt ccccctgctc ctagcaacaa
     4081 gcaagagatc aatacgtaag attggaaacc gttgctacca gtcattggta cggaagcttt
     4141 ttgattattt ttgatcgtga ttcggttttc ttctttgtat gaatctttgt ctactttgaa
     4201 agagatctct tttgttaatt tttgatagtt ttcaggagct tttgtttctt ccagtgagta
     4261 atctccttca gttaaaccag tgatttcaaa atgaccctct ttatctgatt ttaggattgc
     4321 tgcatttgct acatcgtcta cccaaactgc ttctttgaca ccatctttat ctgtatcagt
     4381 aagtccaccg tagtacttac cttctgcatt cttagctgca aattctgcgc cttcaagcgg
     4441 tgttgctttg atacctgttc caaataggcc gctatcgact tttaagaatt ttgcgccata
     4501 agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattattgtt
     4561 ataaacgaaa tccacgctat ttgttaatgg atttgcgatt tcagcactat cattcacaaa
     4621 catgttataa gtcattttta gatcttgacc atgtaaattc gcaactgtat cacttacttg
     4681 tccattattt acgataaaat cgatcacaaa accactatca gaattttcga ttttatagtc
     4741 agtattttct tttaatacga ctgaaccatc attatttgtt actttaatat ctgctttttc
     4801 agcaacataa gaaagtcctt tacctgctgt atctccccat ttaaatacac tgtaaggaga
     4861 agtttcgctt ttcacaccgt ttactgtgat accgtctgcg ccacctgcaa tgtctgttgg
     4921 aatatgaatc gttccttgat aatttactgc gtcaccaatt tggaaagaat gttcttgtcc
     4981 tgttccattt gttactgttt tatcgatcac tggtgcttta tagtttacgt atgaagcttt
     5041 taagtcactt gtcacgccat cattcgtaat ttcaaaggtt aatttattgt gcgcatcgtt
     5101 gcgagcgtct gcgcctaata aatattgatc tgctgttggt tcttgatctg atcccactac
     5161 aacttccgaa ggaacttcca caaaatagta ttttcctaac gtcaaatcag tcgcagtcag
     5221 ctttccttga tcatccgata caagatcacc caattttgtt ccttttccag gttcgccctt
     5281 gtataactca aattttgccc cttttaaaag ggtttgtttg ctttggtctg cgccatcgtc
     5341 accaaatttc gttaattcaa aactcaactt agaaacaatg ttcttaggat acaaatgaat
     5401 attttttaaa aatcctgatc ctgttttatc tgtcattggc gcaatcacaa ccattggttt
     5461 tgctttttgt gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc
     5521 tttggaattg acataggctg gttgatcagt aaaagtgatc tcaccatttt catctacagc
     5581 tgaagtaatt ttgttggttg cattcttgac gtactcactg ttagatccct tgtcttctac
     5641 atcttgtaca atctcgtcca ctttcgcgtt ggttagatca gaatcttctc tcggtaatac
     5701 ttgatccgta atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt
     5761 cgttacacct gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc
     5821 aacattcaat tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc
     5881 tggtggtgtt ttttgcgtga catcttctgc atgtacttga tacccctgaa agcagcctgc
     5941 aatgattcct aacattccaa caagcattcc agcttttttt acataccgat ttattgccat
     6001 tttttactct cctcatttca tatttcaacg actcgcgttg attaaaacct tgttattgta
     6061 gctctccctg taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg
     6121 ttaataaggg tttctctcct ttctttggca aagacaagaa agagttctct tcaggaacgc
     6181 catcgattaa tttaaagtac ccaggtacga taaatttacg cgttaccttg taacgataga
     6241 cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga atatcagaaa
     6301 ataaaacacc tggcatttcc atgttatgac ctgctaggac atagtttcct ttgcccattt
     6361 cggcatcttc ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt
     6421 taaaaattgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat
     6481 tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat
     6541 tattgacgtt cgttgcatcc tccttataac tctttgcatg tgcattgatt tgatctacgg
     6601 gcatcttttt ttctatattt tgcttaaaca cctctccttc atgtttttgg tagctaacat
     6661 agccaaacac actgcaaaag acgattaaag tgactcctaa aaaaccaaat aaccattttt
     6721 tcatccatct gcctcctttc agacaaaaca aaaaaacttt ttctcatttc catagaaaaa
     6781 gttttaaaaa tgaattctat aagttcgtct caactgttca aagtttttca ttgaacctaa
     6841 caaacaagct ttctcttttc ctgttaactg catatcattg atatattgcg cgacagataa
     6901 tcttttaagg tgtttttgct tcatcgtttt tagtaacttt tcttccattt ctttcgacac
     6961 tttttttctg ttttcgattt cttcataatt catgagtttt cctaccttcc ttgttttttg
     7021 caaaaataaa aaagcctatg atcacatacg ctctacactt cagtcttggg ttatcttttt
     7081 ctttcgttta attactggcc gtgctggttt caaatccttg attccattat gggttctcca
     7141 ataatcgaat tcttttcgca atctttcttt ttccatatct gttcctttag ataaggcttt
     7201 gaatacttct ttaggtgaat aaccacgagc taacagctgt acataccttc cttttgtcaa
     7261 agtatccata tccatcgcct ctaaaactgg tcgcgccatc ta
//
LOCUS       DACECB010000098         7250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECB010000098 DACECB010000000
VERSION     DACECB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.100"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWO04_002588"
     CDS             99..746
                     /locus_tag="IWO04_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0214148.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWO04_002589"
     CDS             1139..3784
                     /locus_tag="IWO04_002589"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0214149.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWO04_002590"
     CDS             4094..5407
                     /locus_tag="IWO04_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0214150.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWO04_002591"
     CDS             5397..6050
                     /locus_tag="IWO04_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0214151.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IWO04_002592"
     CDS             6105..6797
                     /locus_tag="IWO04_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0214152.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IWO04_002593"
     CDS             6947..7147
                     /locus_tag="IWO04_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0214153.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2395 a         1229 c         1593 g         2033 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt
//
LOCUS       DACECB010000099         6800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.101, whole genome shotgun sequence.
ACCESSION   DACECB010000099 DACECB010000000
VERSION     DACECB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.101"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..813
                     /locus_tag="IWO04_002594"
     CDS             <1..813
                     /locus_tag="IWO04_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0214154.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IWO04_002595"
     CDS             807..1229
                     /locus_tag="IWO04_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214155.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1531..1911
                     /locus_tag="IWO04_002596"
     CDS             1531..1911
                     /locus_tag="IWO04_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0214156.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1893..2807
                     /locus_tag="IWO04_002597"
     CDS             1893..2807
                     /locus_tag="IWO04_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0214157.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELVHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3047..3736
                     /locus_tag="IWO04_002598"
     CDS             3047..3736
                     /locus_tag="IWO04_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214158.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GFIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3857..3943)
                     /locus_tag="IWO04_002599"
     CDS             complement(3857..3943)
                     /locus_tag="IWO04_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0214159.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4565..5302
                     /locus_tag="IWO04_002600"
     CDS             4565..5302
                     /locus_tag="IWO04_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0214160.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5330..5869
                     /locus_tag="IWO04_002601"
     CDS             5330..5869
                     /locus_tag="IWO04_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0214161.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            6459..>6800
                     /locus_tag="IWO04_002602"
     CDS             6459..>6800
                     /locus_tag="IWO04_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0214162.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2586 a         1125 c         1195 g         1894 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     1501 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
     1561 gatcattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
     1621 gaatctgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
     1681 ttcgtaaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     1741 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgctaag
     1801 tggtgtaata cacaccaaca cgacgttgct cctggagacg cccaacgctt atccgaaaac
     1861 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agccgaacca
     1921 cttcttgtag tcgtgcaatt aattacgctg aaccacgagc aaccattaaa acaggaataa
     1981 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
     2041 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
     2101 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaatag
     2161 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     2221 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
     2281 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaagc
     2341 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     2401 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     2461 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     2521 cttacgaatt ggtccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     2581 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     2641 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     2701 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     2761 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     2821 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     2881 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     2941 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     3001 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     3061 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     3121 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     3181 ctttatattt tacttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa
     3241 gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga
     3301 taaaaagaaa aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttatga
     3361 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     3421 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     3481 tgagcgaacc aaaaaagacg tgcaatatgt aaaagataca aagatagaag aagcctgtag
     3541 tgatctattt ggagacacaa acccttggtt tgccagtgaa gaagaaaaaa aagaaaaaca
     3601 acaatctgtc acatatgaac gctatgaaat cttgcttcaa caagcaacta acatgcaaaa
     3661 tccgacaaaa aaagaaaccg ccttaaacta tctaaaaagt gtcaaggaat ggttagatca
     3721 gcaacaacaa aattgaattt gatttaaaat catgttaaaa tgaatgtgca aatgatcaaa
     3781 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat
     3841 gtcgccttta ttgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga
     3901 cacaaactcc tacaagaagg gggcaaatga acagttcaaa cattagatac acctccttcc
     3961 agtcgcaaga ctgtcggata ggcgacaagt tattatatct taaaaaatta aatttgtgtg
     4021 gtgtttttaa gtatcacttg ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     4081 gcttctcgac tagattaagg gaattaatca agaattatct tagaacggct tagacaagct
     4141 ttcagagcct cacacctcct ctttgaacaa cggttttagc aaaggagccg gaggcgactg
     4201 cgcagtataa tatgagagca tagcgatcac acctttttag cgaccgtagg gagcaagttt
     4261 tccagtttag aaaaattcgc gaaccttttt ggaggaaaaa gtaggagttt tttaggcggg
     4321 aaattcaatg tgcttttaag atatactgtt agatatactg ttagatatac tgttaataat
     4381 aactgttagg gattagcaac cttatgggag agtaagtatt gacataggta gtgggggata
     4441 aattgtcaca ctatggggga taaattgtca cactatgggg gataaattgt cacactatgg
     4501 gggataaatt gtattttaca tcctcttatg tctatgataa cataatttat tgaggtgatt
     4561 tttattgagt aacgatatag ttcgatataa taatggatta aattctgtcc ctttaagaaa
     4621 atttacccct atagatatgg atttattttg gggaatatgt tctaaaatga aaagaaaagg
     4681 tacaaaagaa gaaattttta catttaaaca aataaaagaa ttaattgatt acaatataaa
     4741 tgcacgtggc gaccgttttg ctaatgattt aataaaaatg tcaaaaaaaa tcattacttt
     4801 gtcttttatt tatgaagacg aaaaaacata tgaacaatta aatctgtttc aaaaatttaa
     4861 aatcgataaa gtgaatgaaa cattattaat aaaggtttct gaagaatttg aatatatact
     4921 taattcaatc ggaactaatt ttactcgttt cgaattagaa aatatgacaa acttaaagag
     4981 tagctacgtt aaagagttat atcgccaact tatggctcat aaagacagaa gatctaaaaa
     5041 aggtcactgg tttgtaaaaa tagaagattt tagagctcta ttagcaatac cagaaagtta
     5101 tcgcatgaga gatattgata aacaaatttt tgcccaagcg aaaaaagaat ttttaaacgt
     5161 taattcaact caccctgcta tttttaaaac ttttgaaatc aaaaaaatta aagcctacaa
     5221 aggaaataag ataggatctc tgcaaattta ttttgaagaa aatatacctt caattacctt
     5281 agataattgg ttagagaaat aatacctaac aatttaggag gcaattatca tgcaacccaa
     5341 agaaaccttt acaataaagg aattatctga attatttgaa ataagtaggc aagcaatctc
     5401 taaacatgtt gataaattag acccgtcctt attagctaaa aatgagcgtg gttataaggt
     5461 tgtattacgt tctggagtat tgcaacttgc tagcaactta ggtaaccaaa ggttactata
     5521 taggttgcat gagtatgaac aagaaaacga taaaaacgtt tatatatcaa gcaaaaacgt
     5581 gattgtaaac gagttgctag ataagttgcg tgaagaaaat gaatttttac gagaacaact
     5641 ttccctcaaa gagcaaaata attctaatat gcaaaaatta ctcgaccagc aacaacagct
     5701 caccctacag gcaaatcaac agatcgaaaa actgcaagaa cagttacaat tagcttatac
     5761 ggaagaaact ccagaagatg aatcaactac tattctttct gaagaaacag aatcgataga
     5821 aaaacacaca aaaaagaaga agcaaaaatg gtggcaactt tggaaataac tggagggaac
     5881 attcatgaca agaaaaaatc ttctattcgg ttctatttta ctaggaattc taggattcag
     5941 tccgatgtta ttgtcagtaa tcaataaagg gaataaacat ggtgaattga aattgaatcc
     6001 aaattattac aacagtccta acgaagaatt gtttgtaaaa gagacggata aataataaaa
     6061 aagactgctc ttccgagcag ccagttaaaa aataatgagt gtttattctt gaaaaagcac
     6121 gcgcttatga agcgctacca aaaatttacc atattttaca aaaaataaca agtcttaaaa
     6181 aaatgacaga taaaaatgca caaagaatta gatatagata accgcgaaaa atcaagcttt
     6241 agaaagttgc ttttagctag accaattaaa aatcacaaag tgagcgtctc tatccattgg
     6301 tagaggggct acttggcata gtcgacatta tgttaactaa cgctataaat aacgattatg
     6361 ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat tgtatatttt
     6421 aattataggt gtataccact taaatttaac acaaaggcgt gattattttg aaacccaaaa
     6481 agaaaattta tctaggtctc ctcttttcct tgatgattgg aatttttagt ataggaatta
     6541 caaataccta tgcttcagaa aattctaatt tcaatatcaa ctcccaacca attgaagaac
     6601 aagcaattaa ttttgcgaaa actgattttg aacactccat aacttcaatt cttttaatgg
     6661 aaaataacac cgaaactgat attaactata ctcttggttc gccatttaaa atgctcgatg
     6721 gagaaaatac ggcaaatagt tctattttct ttccagtatt ttcagacgct gataatacga
     6781 ttaaatatat ttataccgtc
//
LOCUS       DACECB010000100         6282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.102, whole genome shotgun sequence.
ACCESSION   DACECB010000100 DACECB010000000
VERSION     DACECB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.102"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..219)
                     /locus_tag="IWO04_002603"
     CDS             complement(<1..219)
                     /locus_tag="IWO04_002603"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0214163.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="IWO04_002604"
     CDS             complement(330..584)
                     /locus_tag="IWO04_002604"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0214164.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="IWO04_002605"
     CDS             complement(777..1052)
                     /locus_tag="IWO04_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0214165.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="IWO04_002606"
     CDS             complement(1024..1977)
                     /locus_tag="IWO04_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0214166.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="IWO04_002607"
     CDS             2589..4082
                     /locus_tag="IWO04_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0214167.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="IWO04_002608"
     CDS             4217..4513
                     /locus_tag="IWO04_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0214168.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="IWO04_002609"
     CDS             complement(4537..5496)
                     /locus_tag="IWO04_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0214169.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5679..>6282
                     /locus_tag="IWO04_002610"
     CDS             5679..>6282
                     /locus_tag="IWO04_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214170.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         2029 a         1048 c         1110 g         2095 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acataacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagcaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
     5581 caatcggcgt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     5641 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     5701 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     5761 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     5821 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     5881 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     5941 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6001 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6061 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6121 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6181 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6241 gttatgagta caatgttgat tttggaagat atgacgaatt ga
//
LOCUS       DACECB010000101         5727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECB010000101 DACECB010000000
VERSION     DACECB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.104"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IWO04_002611"
     CDS             196..921
                     /locus_tag="IWO04_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214171.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IWO04_002612"
     CDS             911..1420
                     /locus_tag="IWO04_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214172.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IWO04_002613"
     CDS             complement(1490..2938)
                     /locus_tag="IWO04_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0214173.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IWO04_002614"
     CDS             complement(2938..3654)
                     /locus_tag="IWO04_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0214174.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IWO04_002615"
     CDS             complement(3635..3985)
                     /locus_tag="IWO04_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0214175.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IWO04_002616"
     CDS             4129..4902
                     /locus_tag="IWO04_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0214176.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IWO04_002617"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IWO04_002617"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACECB010000102         5584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECB010000102 DACECB010000000
VERSION     DACECB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.105"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(487..1296)
                     /locus_tag="IWO04_002618"
     CDS             complement(487..1296)
                     /locus_tag="IWO04_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0214177.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1296..2042)
                     /locus_tag="IWO04_002619"
     CDS             complement(1296..2042)
                     /locus_tag="IWO04_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0214178.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2060..2527)
                     /locus_tag="IWO04_002620"
     CDS             complement(2060..2527)
                     /locus_tag="IWO04_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0214179.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2527..2937)
                     /locus_tag="IWO04_002621"
     CDS             complement(2527..2937)
                     /locus_tag="IWO04_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0214180.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2948..3961)
                     /locus_tag="IWO04_002622"
     CDS             complement(2948..3961)
                     /locus_tag="IWO04_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0214181.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4178..4912
                     /locus_tag="IWO04_002623"
     CDS             4178..4912
                     /locus_tag="IWO04_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0214182.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4896..>5584
                     /locus_tag="IWO04_002624"
     CDS             4896..>5584
                     /locus_tag="IWO04_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0214183.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1789 a         1184 c          920 g         1691 t
ORIGIN      
        1 agcgacaaaa accacgagca ggtccattca tcctcaaacc tcggtgatct tcgcggttta
       61 acaccatcag aagaaatgca caaaaataat aaggagagtc tgagacataa ataaattccc
      121 agacttttag tgagtctaaa tccgaataaa cggtgggtcg aaagcaactc tagctgcgcg
      181 tcgatcgcag caatctctag gtgctatgtt cggataaatg gtggtcaaaa gtcagatttt
      241 cggccatttc gtaacagcca accagacaaa aagcgtctgt tttgtcttct actccaattg
      301 ctcaaatctt agcgactttt ggcttggtct tactctaaac agtttgtaag cctaaacttt
      361 atttgctgtt tgtttctaag atcaaagcaa aactactttt tagttggtta cttttacaag
      421 ttctttttac tgttgaataa atgtaaatct taagaacaaa tatttacttc tataattcca
      481 agtttcttaa aaaattccgc caagtgtacc taaaaagcat aaaacaaaaa ttcctagaat
      541 aattgtgatc gttttatgcc ctttggagta aagccagacg cataaaaagg taattcctaa
      601 tggcagcaag cccggaaaaa tttgatcaaa aatactttgt aatgaagttt cagctccacc
      661 aaccgtgaat tttagtgacg tatttaaagt aaccatcgtg cctatcatcg cgccaattac
      721 cgttaaacct aaaatcgaag caccatgaga taatttgtca atcatatggt tgtcttctgt
      781 atcatcaata aatttagttc ctaaaacata gccatatttt gtcgccaacc atttaaaaat
      841 aaaatgaatg gcagtataca aagtaaaata aaggatcatg cctaaaatat tcccttgttg
      901 agcaaaagaa atacctatgc cagccgcaat aacccgaaaa gttccccaga aaaatgaatc
      961 gccaatacct gataaaggtc ccattaatcc tgttttgatt ttattaattg cgtctgtatc
     1021 aaattcagga tttttagcat tttcttcttc catggtgacg gacaagctca taacaaaagg
     1081 cgcaatatgc ggtgtaatgt taaatgtttc cgtatggcgc tgcaacgctg tataataatc
     1141 atcatcgttc ggatatattt tacgtaagga acgttcaata ccattcattt gtccatatgc
     1201 catcatttta tcgaaggacc aagaggattg cataaaaaag gagcgccaaa atatacttct
     1261 taattctttt ttcccaataa cactttcttt taccattata aatcttcctc ctcttcaaaa
     1321 gttagctttt gaacagccag taacgctaag caaagtccga cgacagccac accgacaaca
     1381 tccattttca gatacattac gccgacaaat cctaagaaaa gataaggcat catcgcttta
     1441 ttcgccatca ttttaattaa taacgctagc ccaacacaag aaagcacacc ggccgcaaca
     1501 tcaaagctgt ccaatacaac ctgaggaatc aacttgatca tcgcattgat tgtagacaca
     1561 ccaaaataag tagctacaaa aactaaaatg gccgatggaa ccccaacgat taaaggttga
     1621 gccacataat gatatttttg tgcttttttg aaatcatgat tgactaatgc ctcatccatt
     1681 ttgttgttaa aatacgtaat tggcattgca taggcgaagt tacgccacat ctgaagaaag
     1741 atggacaaag gtaaagtcaa cgcaacggca gttcctactc cttgacccga ttgaatcgca
     1801 attgctgtac ctaaaacact ggaagcataa acttcagggg gcgtcgcact ccctaccata
     1861 attgagccca tgaacatgga ttccaaggtg gcaccaataa taattcctga ctctaaatct
     1921 cccaaaatca aaccgacaaa aggcgctgta aatagcggtc tacccatcat cgatgcacca
     1981 aatgtgtatt catcaataga cgccaaaaaa gctaccagtg cgattaatat tgcttgtatc
     2041 atattcttcc cctcctcttt tagtcatgca aaggattgat ttttttgtcc gttggtacag
     2101 cctgacattg aactgttatc ccgttttcag tcattttttt caccaagtcc agttcttgtt
     2161 cattcaagta aacggaacta gtaatcattt tccgattctc gtttttcttt aaatttccta
     2221 aattcacttc tttaatctca ggtacgtgct gagtaatttc gtaggcatcc tccaccgtcc
     2281 cgacaattaa aaatacttgg tatttcttga ttttttctcc atttaaaaca gagatggcct
     2341 ctgtcatatc cttcaccaca aattttgtcc ctgtaggaac agccattttt agtgtttttt
     2401 tgcgcagatt attggtagcg atttcattac ttgcaacgaa aatacaattt gcacctaagt
     2461 tatttgtcca ggcaaaagca acttgcccat gaacaaaacg ttcatctact cgtaataatt
     2521 taatcattaa aaatcatcct cttctgtttt ctctagcaac tcgttcacca aaactatccc
     2581 ttcattcccg ctattcatag ctttttggat tagttttttc gtgtcctgtt cacctgataa
     2641 gcaaagctct aaaagcagcg gcagatttac accgctgatt aaataaaaat tgtctttacc
     2701 gatttgttcg gagaaagcgt tagacacgct tcctcccagt aaatctaccg tcacaattaa
     2761 ttcgtcatca ggcgctaagt cggaaaatag ctgctctatt tcctggttaa tggagtagtc
     2821 actatctata tacgcagtta ttgctaaaag tgatttttga ggaccaataa taaattctaa
     2881 agtatccttt aagcctttag ccatcatgga atgcgtcgca attacaattt tcctcatagc
     2941 tcttacctta atattccaca gtccgataat aacgacgaat atctagtgaa tgatcccgca
     3001 ccttttcaaa atgagcactc actcgttcta aggccgttgc cataaccatt ggcgctacat
     3061 actgacgcat agcgcttggt acacctgcta attcgaaatc cgcagtgtca aaaaccgtca
     3121 caacatcgct atatttattt acaaaacgct ctacgcgatc gactagcgga cgcgtttgat
     3181 cttcaccttt taataacaca aaactcatag atttatcagt catttcaata gtcccatgga
     3241 aaaattcggc tgcatggatc gacttggtgg caatccattg catttcttcc agtacgcaca
     3301 tagcatattc ataggcagcc ccataagtat tcccggctcc aacaaacata tgaaattccg
     3361 tatttttatg tttttctgca aattcaagtg ctttctgatc attttgttgc cgaacactta
     3421 gtaatacttc cggcattttt tgtaatgttt gaataaattc atcatattca ggaaactcac
     3481 cgttattttt catcaggcgt gcacccaaag caaaaaactg taactgaagt tcttctacta
     3541 atgtatcgtt gtcagcatca ttttcaatcg cataatccac gatttccccg agtggtgtag
     3601 cagcatttcc tacaaacgca atcgtcgttg caccttgact attggcatac ttaacaacat
     3661 caacggtttc ttgagtcgta ccggttaatg aagatgtgat taacactgta tctttattaa
     3721 tctttttcgg ctgtgaacaa accacctctg cggctatttc atgataataa tcaagctctg
     3781 aatgagtgct taacagataa gctagcgggc tgattgtggc atatgttcca ccagagccta
     3841 ccaaataaag atttttaaat cccttctcac tgattgcatc tgccacctga ttgattgtct
     3901 cgatattttt tactgcacca tttaatactg atagaaaacg ttcttcgcta aaacctaaca
     3961 tagttaaaaa cctccaagtc aataatttga aaaatatatt tgcaaatgcc ggcacattaa
     4021 caaaccactt aacctttttt atataccacc tgttaacgtt tttaatgtac cacttataga
     4081 aaacgctgtc aatagttttt aaccactttt atttttattt gtaccaaaat ccttttttca
     4141 tgtataataa acaaaaatag actctggagt gtaacgtatg aatactgaag aactaacgaa
     4201 caaattaata caagacattg aacaagaaaa atataaatac gcgcaagcta ttccttcaga
     4261 acgtgatttt gttcaaattt atgaaatgaa tcgttcagcc gtaaaaaaag ccattcaaca
     4321 attagtaaat aaagagtacc tactaaaaat acatggcaaa ggaacttttg ttaaaaaaaa
     4381 tatccatgaa catttgcaaa tcaaatttca tggtttgtct gaattgttgg aacgggaagg
     4441 gattcagcct agcagcttaa ttttagcttc agaaataaaa ccggctggac acattgtcgg
     4501 gaaagcttta cagctggaac caactgaaga agtatttcat ttggttcgtt taagaagcgg
     4561 cgacggtgag ccaatttcta ttgaagacac ttatattcca ttaaatctca tacctgatct
     4621 tcaagctttt gatttccaaa ttttttcatt ttattccaca ttggctgctt atcattatca
     4681 aattgaaaat atctatcaga caatttccag ctcaaaagtg agcaataaag aagcccgttt
     4741 gttaaaaacc agcttaaatc gtccggtaat cagccttgct atcactgcgg ttacgacaaa
     4801 tgaacaagcg gttgaataca ctgaagtagt ggttttgcct gaattcgccg ccatctatac
     4861 taaaaatgtt catcaaggaa aggagatgaa aatctatgcc caaattgatt aatctgttgg
     4921 cttatgacct gattgacaaa attattgctt atattcatga acatcattta acccccggaa
     4981 caaaactgcc ttcggaacgg gacctatcag aaatgtttaa tgttaatcga actaccttaa
     5041 gaggcgcgtt acaaaaactg gtggacaacg gcaccttacg ggcgcaaacc aatagcggct
     5101 attatttaac tgcgccaaaa ttcgaaaaaa atgcccatca tttctttaca ccgatagaag
     5161 attctctgct tagtaagcag cggctacagc taaaggcaac ggctctttca ccgaaaaaag
     5221 cagcagctct ttgtccggaa ctgaatgatt tcaaacagca gcaaccacaa gcttatttag
     5281 aatatattga tgcagaagca attagcttga tgttattaat ccctatggaa acattaaaga
     5341 aaagtaattt ctcggacaat cctttccaag attttaaaag tttggcatat acgcgttctc
     5401 aagaaatttc tttaataact gacaatgacg ttgcggcgga cttgctggga gttgacaaag
     5461 aagaggttct gttatttgta cgagaggaaa taagagccgg taaggagttg gcggcgattg
     5521 ctttaggtta tactattagt cgccgctgca acatacaggt taatgttgct acgaccttgc
     5581 aatc
//
LOCUS       DACECB010000103         5469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECB010000103 DACECB010000000
VERSION     DACECB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.106"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            67..351
                     /locus_tag="IWO04_002625"
                     /pseudo
     CDS             67..351
                     /locus_tag="IWO04_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            365..1054
                     /locus_tag="IWO04_002626"
     CDS             365..1054
                     /locus_tag="IWO04_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214184.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1179..3530
                     /locus_tag="IWO04_002627"
     CDS             1179..3530
                     /locus_tag="IWO04_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0214185.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3610..4005
                     /locus_tag="IWO04_002628"
     CDS             3610..4005
                     /locus_tag="IWO04_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214186.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4330..4947
                     /locus_tag="IWO04_002629"
     CDS             4330..4947
                     /locus_tag="IWO04_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214187.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4988..5287
                     /locus_tag="IWO04_002630"
     CDS             4988..5287
                     /locus_tag="IWO04_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214188.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5321..>5469
                     /locus_tag="IWO04_002631"
     CDS             5321..>5469
                     /locus_tag="IWO04_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0214189.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRR"
BASE COUNT         1897 a          735 c         1115 g         1722 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aagaattgta taaagaattt ggtgatttat ccggggaaca gttagaagaa
      121 agtattccag gaattagtga ctcagataaa attgttgtct tgtctgctgg tgggcaatta
      181 atgggagatg gcaaatccga attagttagt tataaacaaa atcacagaca gtttattgat
      241 gttaatactg attccaacgc tatttcgatt aaggatctaa aagagctttt aaatagtaaa
      301 agaaatgaat taaagcagat aaaagaaact aattcagtgg aattagagta aaggaagatt
      361 cattatgaaa aagatttttg gtgtttctgt tgggctactt atgatgttaa catttggaaa
      421 tgcagtatat gctgctgaga aagaagttat ttcagatcca gagcagtacg cctcacgata
      481 ctttgctgat tttgaagaag aagataaagt acttgcattg ccagaaggtg gttttttaaa
      541 aggggaagct aaaattgtat ctatggataa tccagatata attcaaggtt catataattc
      601 agaaactgat cctgcttcag tttcagtgac agaagcaaaa aaaattgttg ctgaaaaatt
      661 caaaaatgat attgaaacta ctccaattat gcctcgagga gcatttccac ccgatgttgg
      721 tactagcgga caccttcttg tactagcagg aggaggaagc tataaatcac agccatttag
      781 tggtgcggga tggcgttttg gaggtatggg tgtaaaacca gctgatggta caggcaactt
      841 tttacgttgg aaatcctaca ttgacgatgg aagagtagga aatgtaaatc aagccaacgc
      901 aactaaatct ggttctattc aaggaatggc attgtatgct cgtgaaggat tcaaatactt
      961 tgcaccaaac gggcctggtt ggacaatgaa tataggtatt tattacactt ataatcctgt
     1021 tgcaggtacg tattatgttg ttcaaaatat ttaataaaac agcacaaaga gatttctttg
     1081 tgctgtttta ttatttttag ttgttgacaa acactaaaat ataaaatata atcaacacat
     1141 aaacgaacgt atgttcggca agaaagcagg ggatttttat ggatgagatg gttttaagta
     1201 ctcagaaatg gctaaacaag aaatacagta atgttacagg gtttgataag gttccagaaa
     1261 atgggcgaac agggtggcct acaatctatg gacttattga aggactacaa gtagaacttg
     1321 gtattactaa tctagtagct aattttggac ctaccactga aaaaatgtac gataatcagg
     1381 ttactcctaa atggggcaaa aatctaccta aaaatattgt atttcttatt caaggtgcct
     1441 tttggtgtaa ggggattaat ccaggtggct tcgatggggt atatactcca tatttagata
     1501 ctgctgtaaa agagttgcag actgatgcag ggtttaatgg aagcgctgta actggagttt
     1561 tggatgcgaa atgggccaaa gcgttatttg atatgagtgc ttttgttctt gttcccggcg
     1621 gagatagtaa aattagaaaa atgcagcaat ggttaaatgt aaattattta gaatacacgg
     1681 gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa tcaagcatta atttttgcat
     1741 tacaagctga actaggatat tctcccagtg aagcaacagg gtcttatggt aatgggacaa
     1801 ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa ctatgtaaga ataattcaat
     1861 atggattata tgttaatggt tattttgaag acggtacatt cgatggtatt ttcactaaat
     1921 atatgggttt agaagtactg gcattccgaa aatttatggt attgcctgaa tatactgaaa
     1981 tagcagatgt tgttgtaata aaaggactat tgtctagcgc tggagatatt agaagatctg
     2041 cagatggcgc agatacttct acgcaattaa ctagatctca aatccaaaca ttggttgata
     2101 atgatataaa aatagttggt agatatctta ctggtactgt tggaattgga aaagatgaac
     2161 gaaataagta tctaactaca gaggaattga ataatatttt ttctaaaaat ttatctgtgt
     2221 ttcctatcta tcaagatgga ggtgcagcat tagcttattt tacttatgat cgtggcttat
     2281 cagatggtaa gaaggctata gatgcagcaa aaaatctaaa tataccatta acaacagtta
     2341 tttattttgc tgttgatcta gacatgttag gggaagaaat tcttgcatat gctggagaat
     2401 attttaaggg ggtatctgct gtaatgtcat atagcgggta tcaaacgggt gtttacggaa
     2461 caagaaatgt ttgttctcaa ataatcaata acgggtatgc ttcgtttgca tttgtttcag
     2521 atatgtcgac tggttatagt gggaatttag gttttcctat gcctagacaa tggtcttttg
     2581 atcaatttgt tgaatttacc ataggaagtg gcaacggagc tgttggtatc gatttaatcg
     2641 ctacatcggg tagagatagc ggatttaatg agttatcaaa tgatactaat aatgataatt
     2701 acattgccaa atacaatcag aaggttataa atcagatggt acgtgcgtat caatatctat
     2761 cacaagctgg tcttgataat ccttggaacc ctcatttgac attttataga tatgtaaatt
     2821 attctggttt gtcatgggac atcatttctt ctcctgtaac agagcatgat agaaaaattt
     2881 atgatgaata taggaataaa ttaactaata gcgagggcct gtataattat tttatagatc
     2941 ctaatactgg ttcaataata ggtttgcctc acttaattgt gacgttacaa tcacagatgt
     3001 ttctagccga cacaatcaat gatagtgttt ctgactttgc aggttggtta ggagatttaa
     3061 tgacttgctg gggtgaagtt aagaaattag gaatacaaat ggagcaaggt gtatttgcat
     3121 tagtaggtac ggctgcaggt ggaacattta gtctagaaga tttataccaa gatgttgacg
     3181 cggtaaatgt ttatacctta gcaaaaagaa attctgattc aacagcttta tcggctttaa
     3241 aaacgtacta ttgtgataag ggtgctattg atagatttaa tcaatttgtt aagaacagat
     3301 ttggttcttc agataaaatc gcttcagtaa caaatagttt attagaaaat gacgaaacag
     3361 ttgatatctt aggtgtaggg ataatttcat ttttcattga taaaatagtt aataattcac
     3421 ttgggacaga ttttacttat cttgatttta ttggagatga ggggaaagtt gcaaatggat
     3481 tttctgaaaa aattattcaa tttcaagaaa atagaagtaa acttaattaa gaatcgttta
     3541 ctatttaaac agtgtccttt tcctttttaa atatatacga taaaataagt atatgaattg
     3601 taaaaggaca tgatgattat ggaagaaata gaatggaata atctgactca aaataataaa
     3661 aataattctg gatgtggaat tatctttatg tttgttatag ttttgggaat agtagtactt
     3721 ttaataactg tcaagatagt agattacttt gcgaatactc ctgaagaaca aatacagaga
     3781 aaacttcctg agtttgaaag agtaccacct tatgatccaa gagataattc atttcaagta
     3841 aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag ttacagttga atcaagcgac
     3901 ttcaaagaaa aaaagaaaga atacaattat attgtaaagg caaaagtatc aaaaagattt
     3961 atttttaacc ctaaattaga aatatatagt gttgtaatag aataatagtg ttaaaaaaat
     4021 tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata
     4081 aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat
     4141 gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat
     4201 actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa
     4261 tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag
     4321 ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa
     4381 cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa
     4441 gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa
     4501 ggagatttac tctatctaca atcattagat agactaggca gaaataaaca aatgatttta
     4561 gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct
     4621 ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta
     4681 attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa
     4741 agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct
     4801 ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc
     4861 tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat
     4921 gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag
     4981 tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac
     5041 acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac
     5101 ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct
     5161 ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat
     5221 tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta
     5281 tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga
     5341 tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga
     5401 ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca
     5461 tcgccgata
//
LOCUS       DACECB010000104         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECB010000104 DACECB010000000
VERSION     DACECB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.107"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..1107
                     /locus_tag="IWO04_002632"
     CDS             133..1107
                     /locus_tag="IWO04_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0214190.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IWO04_002633"
     CDS             1089..1550
                     /locus_tag="IWO04_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0214191.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IWO04_002634"
     CDS             1600..2064
                     /locus_tag="IWO04_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0214192.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IWO04_002635"
     CDS             2078..2827
                     /locus_tag="IWO04_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0214193.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IWO04_002636"
     CDS             2827..3657
                     /locus_tag="IWO04_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0214194.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IWO04_002637"
     CDS             3657..4304
                     /locus_tag="IWO04_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0214195.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAISSSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IWO04_002638"
     CDS             complement(4402..5178)
                     /locus_tag="IWO04_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0214196.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IWO04_002639"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IWO04_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          972 g         1883 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat ttcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACECB010000105         5339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECB010000105 DACECB010000000
VERSION     DACECB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.108"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO04_002640"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO04_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO04_002641"
     CDS             complement(546..740)
                     /locus_tag="IWO04_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214197.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO04_002642"
     CDS             2092..3132
                     /locus_tag="IWO04_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0214198.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO04_002643"
     CDS             3956..4288
                     /locus_tag="IWO04_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0214199.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO04_002644"
     CDS             4907..5248
                     /locus_tag="IWO04_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0214200.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaagga
//
LOCUS       DACECB010000106         5206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECB010000106 DACECB010000000
VERSION     DACECB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.109"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..483
                     /locus_tag="IWO04_002645"
     CDS             46..483
                     /locus_tag="IWO04_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214201.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            476..1183
                     /locus_tag="IWO04_002646"
     CDS             476..1183
                     /locus_tag="IWO04_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214202.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            1564..1743
                     /locus_tag="IWO04_002647"
     CDS             1564..1743
                     /locus_tag="IWO04_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214203.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            2084..2347
                     /locus_tag="IWO04_002648"
     CDS             2084..2347
                     /locus_tag="IWO04_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0214204.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2341..2691
                     /locus_tag="IWO04_002649"
     CDS             2341..2691
                     /locus_tag="IWO04_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0214205.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(2715..3329)
                     /locus_tag="IWO04_002650"
     CDS             complement(2715..3329)
                     /locus_tag="IWO04_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214206.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            3643..4065
                     /locus_tag="IWO04_002651"
     CDS             3643..4065
                     /locus_tag="IWO04_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214207.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4075..4278
                     /locus_tag="IWO04_002652"
     CDS             4075..4278
                     /locus_tag="IWO04_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214208.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            4489..5073
                     /locus_tag="IWO04_002653"
     CDS             4489..5073
                     /locus_tag="IWO04_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214209.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1844 a          779 c          981 g         1602 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
      421 gaaagtatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
      481 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa gcaaagaaca
      541 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      601 ctttattcaa agagtggaag aacagttgta tatggaaagc gaaacaagtc aaacgaaggt
      661 cacagtcagc cataatcatt tagacgtgta taaacgtcgt cctgaaacgg taaaaaaagg
      721 aacagagctt ctacgactag aaacagaaaa atacatcatg aatgtgtacc tagaaaggag
      781 tgagctggtc gaaaactttg tcagctacac gatttgtagg aaacactatt actcagaatc
      841 agaatattat tcaattttcg agagcatgga attaatggaa gaagaaatgg aagagctaaa
      901 cagcaaaaat gcgacggtag acgattatta ccttgtattt actaggtttt tgacgtgtgg
      961 gacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg aagcattttt
     1021 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     1081 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     1141 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa taaccattta taccgaaaag
     1201 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     1261 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga
     1321 taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg
     1381 tttgttggcg agatacttat gcgtcatgct gcagctgtac atttaaactt cgtagtattc
     1441 ttaggaatac tggtattttg tttgatcttt tggcgatttg tattagccct tgtgagtcta
     1501 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagagagta
     1561 ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga aaagagacaa
     1621 aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg aatctaagat tatgtgtatt
     1681 gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atataccagc actaaaaaag
     1741 tgacgtcact tttcatttga agaaacaaaa gtgatagcac ttaattgttc ctactattta
     1801 gaatattttc tatgtccttt ttattccatt ttgtatcaat aactatgaaa tctttaaagt
     1861 atccagaaga gattattttt agatcaattt ttttcttacc tgattctaaa gattttgttt
     1921 tcttaaacat attatgattt cctttcgact attttcatat aaatgttaac atagtttatg
     1981 gattcatgga atgttaatct aatttaatag aaaaggataa ttaagtatat acgttttttc
     2041 ttatgtatgt tatactatgt atatacagaa agaggtgtct actatggaaa tgttacctat
     2101 aaaaaaatgg ggaaatagta atggccttag attgccaaag tatattatgg aatatcttgg
     2161 aattcataca gaagacaaag tgaagataat acaagaagaa gtaaacggtc agaagcggct
     2221 aattatcgaa gcggctaata tagaaaatga attaacaatt gaacagttat ttgaaaatta
     2281 cactgatgaa agaaatcatg tgacgattca aaatttaggt gaagcagtag gtaatgaaaa
     2341 atggtgagaa aacctagaca aggcgatatt ctattattga atacagcacc tcgatctgga
     2401 cacgaacaga caggtaaaag accttatatt gttttaagtc atgatattat tgcggattat
     2461 agtaacgttg ttacagttgc tccaatttct agtacaacta gaaactatcc gctatatgta
     2521 aatataaact cagaatatga aatgaagaca acaggaaaag tattattaga tcaactaacg
     2581 acaattgatt atgaagccag agagtgtatt tttttggaaa aagcgcatga tagtttagtt
     2641 gaggagttat tgatgaaagt tagaacagtt tttcaaaaag taagcaaata aaatagaaac
     2701 agcagcatga tcaactattc gtgattgacc atgctgtctt tttttattct atatacggtt
     2761 tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg caacaccttt ttttagatcc
     2821 tcaacgatgt ttttataaac aagacgacgt tggggatctt ttgcattggc actgtatagc
     2881 ttaggacgtc ctttataaac accgttcgct tttgcaatct taatgccttg tgcctgacgt
     2941 cgtttagatt cggtacgttc ttgttcagcg atcatggcca aaatttgaat gatcaaatct
     3001 ttaatgaatt tatccaggag aggattgcca attgcttcgg ccatgatcgg tagacttgtg
     3061 atgatcagct gcacattttt cttcttcaag taattgactg aagcgatgat ctcgtcgtaa
     3121 tttcttccaa gtcgatcaat agcttccaca ataaaaatat cttgatctcg gacgaaatcc
     3181 agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc cagattgttt ctcaatgaaa
     3241 attctctctg caccgaattt tttcatttct tcaatctgcc gttgttcatt ttgatcgatg
     3301 gatgagacac gtatgtatgc aactctcaaa atttttacct actttctgca actatttaaa
     3361 aaacacccta atcatacacg agaaaaccag atttttcaat gtcgcggtcg tgtctagtta
     3421 gggtatacct tatatagaca aaaactaatg atggaggaaa tagtatgtgt cacttttctt
     3481 tttaaatggc aatatttttt gtaaataaat gtcaatcaag aaaatttaaa cgattatgac
     3541 attaaaaata tattttatga attttctagt atataacttg atttatctgt taagatttat
     3601 taagaaaacg ttttcttatt tttgatttcg aggtgataga caatgaaaaa taagtttatt
     3661 ttatctttag gtattttaac tattggaatt agtgtaactg gaactacaat tcctacattt
     3721 gctgatagta caaatgaacc aactactgta gtagataata accaagaatt aactccttta
     3781 gataggaacg gaatagccat tggtaaccca actaacgact taactgttac tgaaaattat
     3841 ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt gacaggaacg
     3901 atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa ttggaaagaa
     3961 aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa atcagctgga
     4021 aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt ttttatgcca
     4081 gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa aattaattta
     4141 ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca aaaaattcaa
     4201 aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca
     4261 tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt ctttttttct
     4321 aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg ctttattttt
     4381 caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt cagttaaact
     4441 atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt gaaaatagga
     4501 tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag cctaaataaa
     4561 tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa tcgaccagga
     4621 ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat
     4681 cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa tgagcgtggt
     4741 gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc tacaggacag
     4801 ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat cctggaacga
     4861 tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa
     4921 ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac acctattaag
     4981 actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt ggcaaaaatg
     5041 gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc tgaaaatttt
     5101 tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt ctactgataa
     5161 cttattctca aatgttgtaa ggattttatt catttgttaa tattaa
//
LOCUS       DACECB010000107         4955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECB010000107 DACECB010000000
VERSION     DACECB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.110"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            209..307
                     /locus_tag="IWO04_002654"
                     /pseudo
     CDS             209..307
                     /locus_tag="IWO04_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(530..760)
                     /locus_tag="IWO04_002655"
     CDS             complement(530..760)
                     /locus_tag="IWO04_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0214210.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(990..1808)
                     /locus_tag="IWO04_002656"
     CDS             complement(990..1808)
                     /locus_tag="IWO04_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0214211.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1969..2658
                     /locus_tag="IWO04_002657"
     CDS             1969..2658
                     /locus_tag="IWO04_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0214212.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2672..4174
                     /locus_tag="IWO04_002658"
     CDS             2672..4174
                     /locus_tag="IWO04_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0214213.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4187..4663
                     /locus_tag="IWO04_002659"
     CDS             4187..4663
                     /locus_tag="IWO04_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0214214.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4822..>4955)
                     /locus_tag="IWO04_002660"
     CDS             complement(4822..>4955)
                     /locus_tag="IWO04_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0214215.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1654 a          719 c          883 g         1699 t
ORIGIN      
        1 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
       61 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      121 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
      181 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
      241 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
      301 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
      361 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
      421 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
      481 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
      541 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
      601 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
      661 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
      721 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
      781 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
      841 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
      901 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
      961 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1021 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1081 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1141 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     1201 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     1261 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     1321 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     1381 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     1441 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     1501 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     1561 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     1621 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     1681 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     1741 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     1801 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     1861 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     1921 tttggagtta ttatccaaaa ataataaaaa attggagcgt ttaggataat ggataaaaag
     1981 aggtttttag atcttatttc aaaaggtttg attgtttcat gtcaagcatt acctggtgaa
     2041 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2101 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     2161 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     2221 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     2281 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     2341 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     2401 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     2461 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     2521 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     2581 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     2641 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     2701 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     2761 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     2821 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     2881 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     2941 tggtggagta gcttatttag tatatactgc aactattagt gggtttcttg aacttttttc
     3001 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3061 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3121 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     3181 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     3241 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     3301 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     3361 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     3421 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     3481 gtttgggtta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     3541 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     3601 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     3661 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     3721 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     3781 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     3841 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     3901 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     3961 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4021 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4081 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4141 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     4201 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     4261 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     4321 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     4381 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     4441 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     4501 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     4561 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     4621 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     4681 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     4741 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     4801 gtaatttaaa gttatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct
     4861 caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat gattacgttg
     4921 attgctgaca ctggaaataa atgtctgatt cactt
//
LOCUS       DACECB010000108         4931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECB010000108 DACECB010000000
VERSION     DACECB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.111"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWO04_002661"
     CDS             85..972
                     /locus_tag="IWO04_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0214216.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWO04_002662"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWO04_002662"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0214217.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWO04_002663"
     CDS             2274..2720
                     /locus_tag="IWO04_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0214218.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWO04_002664"
     CDS             2993..4345
                     /locus_tag="IWO04_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0214219.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IWO04_002665"
     CDS             4516..4803
                     /locus_tag="IWO04_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214220.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DACECB010000109         4805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECB010000109 DACECB010000000
VERSION     DACECB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.112"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO04_002666"
     CDS             <1..411
                     /locus_tag="IWO04_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214221.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="IWO04_002667"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="IWO04_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0214222.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="IWO04_002668"
     CDS             2446..3708
                     /locus_tag="IWO04_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0214223.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4805
                     /locus_tag="IWO04_002669"
     CDS             3953..>4805
                     /locus_tag="IWO04_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0214224.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          980 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaagaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaa
//
LOCUS       DACECB010000110         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECB010000110 DACECB010000000
VERSION     DACECB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.113"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            321..1052
                     /locus_tag="IWO04_002670"
     CDS             321..1052
                     /locus_tag="IWO04_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0214225.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="IWO04_002671"
     CDS             1197..3224
                     /locus_tag="IWO04_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0214226.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="IWO04_002672"
     CDS             3332..4429
                     /locus_tag="IWO04_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0214227.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gttgg
//
LOCUS       DACECB010000111         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECB010000111 DACECB010000000
VERSION     DACECB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.114"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWO04_002673"
     CDS             574..1008
                     /locus_tag="IWO04_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214228.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWO04_002674"
     CDS             2298..3221
                     /locus_tag="IWO04_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0214229.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWO04_002675"
     CDS             3237..3776
                     /locus_tag="IWO04_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0214230.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACECB010000112         4424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECB010000112 DACECB010000000
VERSION     DACECB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.115"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO04_002676"
     CDS             690..1658
                     /locus_tag="IWO04_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0214231.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO04_002677"
     CDS             1669..2484
                     /locus_tag="IWO04_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0214232.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO04_002678"
     CDS             2552..3262
                     /locus_tag="IWO04_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0214233.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="IWO04_002679"
     CDS             3298..>4424
                     /locus_tag="IWO04_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0214234.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DACECB010000113         4415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECB010000113 DACECB010000000
VERSION     DACECB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.116"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            58..912
                     /locus_tag="IWO04_002680"
     CDS             58..912
                     /locus_tag="IWO04_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0214235.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1009..3258
                     /locus_tag="IWO04_002681"
     CDS             1009..3258
                     /locus_tag="IWO04_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0214236.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3261..>4415
                     /locus_tag="IWO04_002682"
     CDS             3261..>4415
                     /locus_tag="IWO04_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0214237.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          805 g         1428 t
ORIGIN      
        1 taataatata aacataataa aacaaaaatt atccaaatct aacataggag ggtggatatg
       61 gaaaattcta tatatttagt aacagaacct aacactcagc tgaaagattg ttactttaat
      121 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
      181 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      241 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      301 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      361 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      421 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      481 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      541 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      601 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      661 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      721 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      781 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      841 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      901 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      961 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
     1021 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
     1081 ataagttaca tttttaagat accggaagat ggtaaattat tgcatttata ttatggtgag
     1141 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1201 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1261 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1321 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1381 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1441 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1501 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1561 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1621 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1681 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1741 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1801 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1861 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1921 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1981 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     2041 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     2101 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2161 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2221 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2281 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2341 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2401 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2461 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2521 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2581 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2641 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2701 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2761 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2821 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2881 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2941 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     3001 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     3061 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3121 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3181 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3241 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3301 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3361 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3421 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3481 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3541 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3601 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3661 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3721 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3781 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3841 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3901 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3961 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     4021 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     4081 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4141 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4201 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4261 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4321 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4381 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DACECB010000114         4317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.120, whole genome shotgun sequence.
ACCESSION   DACECB010000114 DACECB010000000
VERSION     DACECB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.120"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO04_002683"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO04_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0214238.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IWO04_002684"
     CDS             complement(734..1573)
                     /locus_tag="IWO04_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0214239.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IWO04_002685"
     CDS             1758..2645
                     /locus_tag="IWO04_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0214240.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3239..3934)
                     /locus_tag="IWO04_002686"
     CDS             complement(3239..3934)
                     /locus_tag="IWO04_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0214241.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3918..4316)
                     /locus_tag="IWO04_002687"
     CDS             complement(3918..4316)
                     /locus_tag="IWO04_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0214242.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDESHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1430 a          852 c          640 g         1395 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     3181 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     3241 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     3301 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     3361 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
     3421 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
     3481 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
     3541 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
     3601 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
     3661 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
     3721 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
     3781 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
     3841 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
     3901 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
     3961 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
     4021 attgttcctg gaagttccgg ctcatgagat tcatcaaatt ttatcgtgat atgtcctaat
     4081 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
     4141 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
     4201 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
     4261 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DACECB010000115         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECB010000115 DACECB010000000
VERSION     DACECB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.121"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWO04_002688"
     CDS             complement(37..1257)
                     /locus_tag="IWO04_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0214243.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWO04_002689"
     CDS             complement(1277..1882)
                     /locus_tag="IWO04_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0214244.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWO04_002690"
     CDS             complement(1931..2863)
                     /locus_tag="IWO04_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0214245.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWO04_002691"
     CDS             complement(3284..4144)
                     /locus_tag="IWO04_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0214246.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACECB010000116         4161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.122, whole genome shotgun sequence.
ACCESSION   DACECB010000116 DACECB010000000
VERSION     DACECB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.122"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IWO04_002692"
     CDS             complement(<1..153)
                     /locus_tag="IWO04_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0214247.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="IWO04_002693"
     CDS             complement(475..696)
                     /locus_tag="IWO04_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214248.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="IWO04_002694"
     CDS             complement(794..1039)
                     /locus_tag="IWO04_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214249.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="IWO04_002695"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="IWO04_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="IWO04_002696"
     CDS             complement(2263..3264)
                     /locus_tag="IWO04_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0214250.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="IWO04_002697"
     CDS             complement(3423..3689)
                     /locus_tag="IWO04_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0214251.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="IWO04_002698"
     CDS             complement(3679..4035)
                     /locus_tag="IWO04_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0214252.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1324 a          750 c          556 g         1531 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga attatggttc tgttgcaaag ttttaaatct actatcaaat aaggtagaat
     4141 aatagaaaaa gatagcagga g
//
LOCUS       DACECB010000117         4104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECB010000117 DACECB010000000
VERSION     DACECB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.125"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..542
                     /locus_tag="IWO04_002699"
     CDS             <1..542
                     /locus_tag="IWO04_002699"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0214253.1"
                     /translation="SKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHT
                     KRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKI
                     RHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHS
                     SIHYLTPQEFEELAKEKMA"
     gene            complement(1015..1587)
                     /locus_tag="IWO04_002700"
     CDS             complement(1015..1587)
                     /locus_tag="IWO04_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214254.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1603..2208)
                     /locus_tag="IWO04_002701"
     CDS             complement(1603..2208)
                     /locus_tag="IWO04_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0214255.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2329..2586)
                     /locus_tag="IWO04_002702"
     CDS             complement(2329..2586)
                     /locus_tag="IWO04_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0214256.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2579..2848)
                     /locus_tag="IWO04_002703"
     CDS             complement(2579..2848)
                     /locus_tag="IWO04_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0214257.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2979..3158)
                     /locus_tag="IWO04_002704"
     CDS             complement(2979..3158)
                     /locus_tag="IWO04_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214258.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(3539..>4104)
                     /locus_tag="IWO04_002705"
     CDS             complement(3539..>4104)
                     /locus_tag="IWO04_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214259.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1225 a          830 c          622 g         1427 t
ORIGIN      
        1 tttcaaaaga gaatctctta aatcaggact tttctactga aacgatctgt gagaaatggg
       61 cagctgacat tacgtacatt cctactaaga aaaatggttg gtgttactta tcttcaatca
      121 tggatttaca cacgaaaaga attattagtt atacattttc aaaacgaatg actgtggatt
      181 gtgtcattca aacattgaat aaagcaaaaa tacattatca cattccagaa ggaatgattc
      241 tacacactga cttgggcagc caatacacag caagagaagt ggaacaatgg cttaaaacca
      301 acaaaataag gcattcttat agtagaaaag gaacacctta tgataatgct ggaatcgaat
      361 ctttccatgc ctcattgaaa aaagaagaag tctacacgac tagctactca gattttgaag
      421 aagcaaatcg agcgctattt agctacattg aaggatttta taaccgaaat cgaatccata
      481 gctcgattca ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa aaaatggctt
      541 aaccgtctct actaaaatgt gtccaagata ttgactcaaa tccagtaata tcacaatagg
      601 aatgctgttt attatattag cagcagtgct gatttttatg atagttattt ggttcttaaa
      661 tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa tagagtcaat
      721 agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac ttacgctgaa
      781 cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga tggtatccta
      841 atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt accatacaca
      901 ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat gctaatagac
      961 tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc ttttttaact
     1021 ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg ttatagcttc
     1081 aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct tcttcggtgt
     1141 gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg caaacatttt
     1201 tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac tcaacaacgt
     1261 ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca aaaccttgat
     1321 cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg tgttacgagc
     1381 aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag ccaacagctg
     1441 attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa tattcttctt
     1501 ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag aagcggttga
     1561 gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat catctatcgt
     1621 atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat ataactcgcg
     1681 atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca aatcgtttat
     1741 gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc aatctacaac
     1801 tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc ggccatttcc
     1861 ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct cttcaaaaga
     1921 agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa ctggtgcaaa
     1981 tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat cgtaaatatc
     2041 cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt cgatttcatt
     2101 tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt cttcaacctt
     2161 agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc gaaatctcct
     2221 taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg attttgttta
     2281 tgtgtcaaaa actattaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc
     2341 ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc
     2401 tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa
     2461 tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct
     2521 tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt
     2581 aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt
     2641 catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt
     2701 gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca tctttacttg
     2761 ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc
     2821 ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca
     2881 attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa
     2941 agtgctatca cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc
     3001 tcaaatcctg ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat
     3061 tcacttttgc cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac
     3121 gtaacgggta accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga
     3181 aataacccta tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat
     3241 acaaagcgcc aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa
     3301 tgcacagctg cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga
     3361 atgattttat tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat
     3421 ttcgcaccac ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc
     3481 cttagttact aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt
     3541 attgcctttt tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt
     3601 ggatttcttc tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc
     3661 agtcgtcgcc tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag
     3721 cgagttcata atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag
     3781 taaatacaag gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt
     3841 cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt
     3901 tcctgcaaat cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat
     3961 tcatgatgta tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag
     4021 gacgacgttt atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt
     4081 cactttccaa atacaattgt tctt
//
LOCUS       DACECB010000118         4034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.126, whole genome shotgun sequence.
ACCESSION   DACECB010000118 DACECB010000000
VERSION     DACECB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.126"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IWO04_002706"
                     /pseudo
     CDS             79..234
                     /locus_tag="IWO04_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..1530
                     /locus_tag="IWO04_002707"
     CDS             253..1530
                     /locus_tag="IWO04_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0214260.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="IWO04_002708"
     CDS             1540..2196
                     /locus_tag="IWO04_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0214261.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="IWO04_002709"
     CDS             2196..3572
                     /locus_tag="IWO04_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0214262.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="IWO04_002710"
     CDS             3702..>4034
                     /locus_tag="IWO04_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214263.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DACECB010000119         3984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECB010000119 DACECB010000000
VERSION     DACECB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.127"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            17..148
                     /locus_tag="IWO04_002711"
     CDS             17..148
                     /locus_tag="IWO04_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0214264.1"
                     /translation="MILKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            394..990
                     /locus_tag="IWO04_002712"
     CDS             394..990
                     /locus_tag="IWO04_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0214265.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="IWO04_002713"
     CDS             1003..1902
                     /locus_tag="IWO04_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0214266.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1905..2036
                     /gene="ssb"
                     /locus_tag="IWO04_002714"
     CDS             1905..2036
                     /gene="ssb"
                     /locus_tag="IWO04_002714"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0214267.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2058..2390
                     /gene="ssb"
                     /locus_tag="IWO04_002715"
     CDS             2058..2390
                     /gene="ssb"
                     /locus_tag="IWO04_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0214268.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2412..2669
                     /locus_tag="IWO04_002716"
     CDS             2412..2669
                     /locus_tag="IWO04_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214269.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2828..2950)
                     /locus_tag="IWO04_002717"
     CDS             complement(2828..2950)
                     /locus_tag="IWO04_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0214270.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3140..3364
                     /locus_tag="IWO04_002718"
     CDS             3140..3364
                     /locus_tag="IWO04_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214271.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3812..>3984
                     /locus_tag="IWO04_002719"
     CDS             3812..>3984
                     /locus_tag="IWO04_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0214272.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1416 a          695 c          804 g         1069 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     2341 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     2401 aaggagtaag catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg
     2461 catttataga cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga
     2521 aaggatcact atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag
     2581 gaaaggataa ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac
     2641 ttacggctat tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga
     2701 aatattgcta taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca
     2761 ccgattaaag aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag
     2821 tagacggtta tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa
     2881 tggtaaacat accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta
     2941 ctcctttcat agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc
     3001 accgtcttca aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc
     3061 tcaattttag ctgtttttgc tataatttta gcgaggtgat cgtgatggaa caatatattg
     3121 gtttactcga taaattaggg tgatcaagac acagccattg cttgtgcgat ttacattacg
     3181 cacgagtcat gaaccaatca attgtgtagt ggccaatata gaaatagcgg ataaattgct
     3241 gattttggaa gaaggcaagt atactatttc agtgacaggt cacttcaata aacgaaatca
     3301 actagtcatt gaatcaatgc aaattagaaa ccctgatcat tttacgagat taatgggcat
     3361 ataaataaaa gaagagctgc agtcctaaat gaggattgca gctctttttg ttttacatag
     3421 aaacaagaaa aatgaaaagg cctttttagg aaggcgcaag cgccttctca attgaaaaac
     3481 ctctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt
     3541 cactaagata aggggtcttg caaagcaaga caaaatagaa aaaacttcct ctcaccaaaa
     3601 ggttcgttcc tgttcgctat ctctttattg atttgccaga ccggcaacaa gttgcctttt
     3661 atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcacgccctt gtccttgtcg
     3721 cttttagttg attcattctg gccaacaaat caataaagaa agaactaacg aaaggaagta
     3781 aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt attggggtgt
     3841 tcccttcagc gaattattcc caaactatca tgcaccatat actgtcgaat gcgattgtgg
     3901 agaaagagcg aaatgtatca aaagctattg tttgtatcaa tgtccaacat gcgggaaaaa
     3961 atataccttg tcttatggcg atta
//
LOCUS       DACECB010000120         3717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECB010000120 DACECB010000000
VERSION     DACECB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.128"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..243
                     /locus_tag="IWO04_002720"
     CDS             <1..243
                     /locus_tag="IWO04_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0214273.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            482..1075
                     /locus_tag="IWO04_002721"
     CDS             482..1075
                     /locus_tag="IWO04_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0214274.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="IWO04_002722"
     CDS             1091..1990
                     /locus_tag="IWO04_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0214275.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="IWO04_002723"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="IWO04_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0214276.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="IWO04_002724"
     CDS             2844..3104
                     /locus_tag="IWO04_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214277.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3545..>3717
                     /locus_tag="IWO04_002725"
     CDS             3545..>3717
                     /locus_tag="IWO04_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0214278.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1348 a          637 c          724 g         1008 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     3361 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     3421 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca
     3481 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     3541 aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     3601 tcatgcacca catactgttg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     3661 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgatta
//
LOCUS       DACECB010000121         3465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECB010000121 DACECB010000000
VERSION     DACECB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.129"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IWO04_002726"
     CDS             137..748
                     /locus_tag="IWO04_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0214279.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IWO04_002727"
     CDS             821..1750
                     /locus_tag="IWO04_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0214280.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IWO04_002728"
     CDS             1908..2645
                     /locus_tag="IWO04_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0214281.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..>3465)
                     /locus_tag="IWO04_002729"
     CDS             complement(2693..>3465)
                     /locus_tag="IWO04_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214282.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1150 a          538 c          660 g         1117 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catat
//
LOCUS       DACECB010000122         3399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECB010000122 DACECB010000000
VERSION     DACECB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.130"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IWO04_002730"
     CDS             <1..277
                     /locus_tag="IWO04_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0214283.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IWO04_002731"
     CDS             420..641
                     /locus_tag="IWO04_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0214284.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IWO04_002732"
     CDS             657..1499
                     /locus_tag="IWO04_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214285.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IWO04_002733"
     CDS             1562..3364
                     /locus_tag="IWO04_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0214286.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          702 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DACECB010000123         3097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECB010000123 DACECB010000000
VERSION     DACECB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.132"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..346)
                     /locus_tag="IWO04_002734"
     CDS             complement(152..346)
                     /locus_tag="IWO04_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214287.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
     gene            594..1735
                     /locus_tag="IWO04_002735"
     CDS             join(594..822,822..1735)
                     /locus_tag="IWO04_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0214288.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            complement(1896..2216)
                     /locus_tag="IWO04_002736"
                     /pseudo
     CDS             complement(1896..2216)
                     /locus_tag="IWO04_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2237..>3097)
                     /locus_tag="IWO04_002737"
     CDS             complement(2237..>3097)
                     /locus_tag="IWO04_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214289.1"
                     /translation="HIHIHFAVVEKENTRKPYTEVENGVSYIVPKGKRRQATLDAMKT
                     NYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYK
                     TLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQISKALKEEAKSL
                     YGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVDQMQSIYNERSFR
                     TLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQ
                     RRIQMEQERQ"
BASE COUNT          916 a          642 c          537 g         1002 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgatttggta gaatctaact actaagaaaa ggacgtgtcc acgatggcaa
      601 aagccaagaa aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg
      661 gccaatcggt cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt
      721 ggaagaagct tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga
      781 tgaaaaagga aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca
      841 tattcgctca aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga
      901 tcatgacgta aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actatgactg
      961 gaagaaaaag aaaccttcca agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc
     1021 aggaatctac tttgaaaaca agggtatcta tggtgcgcct aaaattcata agattcttgt
     1081 tggccgtggc gagaccttgt ccttaaagaa agtacaaata ctgatgagaa aattaggctt
     1141 gcgttcagtc accctcaaga agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg
     1201 acgtaaaaac ctgttaaacc aagattttac cactacgtcg atcaatcaaa aatgggtgac
     1261 agatattacc tatattcata ccctaaaaga tggctggacc tacctctcca ctattcaaga
     1321 cctccacaca aagaaagtca tcggttggaa gtttggcaag cagatgacga aagaattggt
     1381 tatcgaaacc cttgacaatg cgctcttaaa ccaaacaccc tccgagaact tgatcatcca
     1441 ctcagatttg ggttctcagt acactagcga agcctatgag gcaaagctga atgaattgaa
     1501 cattcggcac tccttcagtc gtaagggctg tccctacgat aatgccggta tcgaatcgtt
     1561 tcacgcctca ctcaaaaaag aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc
     1621 tgcttattta gcgttatttc aatacattga aggattttac aatcgcaagc gaatccatag
     1681 tagtatcaac tatttaacac caaatcagat ggaggagttg gcattgcaag cttagttcct
     1741 agctaccact tacatttttt tggcgagatt tccacagggt ttcaacttca tcgcttcgat
     1801 cagcggtcaa acggttttgg tttgaccgct ggtcgaaacg atgagatact cacccgctgg
     1861 aatctcgaaa aaattctcac aaatcgtgtc cgaggtattg actcaaatcc aatgctttta
     1921 catggaaagt tattgattcc ctcttcattc atttcccaag tagtataata aggtgctaac
     1981 ttgccctcat attcttgaat gataaaatat ttttcaccat tcacatgatt acaacaatac
     2041 acaccagtag ctgggtactc aacgactaaa aattgatttg gatcaaactg ttcgtttctc
     2101 aacatttctc ttatctggaa caaatgctct ttttctaaac aatcaggaag ttgttcgatt
     2161 cgttcttctc ttaatgatcc ttctataact ttccattgat ctaaatattg tttcattgct
     2221 attcctccta ctgtctttac tgtctttctt gttccatttg aattcttcgt tgttgctctt
     2281 cgtatgcttt acttgcgtgg tatttttctt catgggcatt aatcgttcga tttaattgac
     2341 gcaaactccg ctgcgtgaca actctttgtt ggaattgttt cctgcgcttc atgaattctt
     2401 cgtaactata tttgtattca ttcgttggca attgtttaag cgttcgaaag gatcgttcat
     2461 tgtaaataga ttgcatctga tcaacacttt ctattaatgg ttttatttga ctatcctgtc
     2521 tcttcagctc tgttaggatg gcatttcctg tgcgcttttt taaatcaaag agagcatttt
     2581 tctctgcatc ctttgattct ctcttgcttt ccccataaag actttttgct tcttctttca
     2641 atgcttttga aatctgtata tattcttgat attcaggttc ttttgttaaa acatatttgg
     2701 ttaactcatc gagtttctct cttgtgtatg gtgaaagttt ggttcgtttt tcatccccgt
     2761 aattccattc cgaacgtttg tcaggtaggg ttttatatac ttcatttaat aagttcatcg
     2821 cgtgtcgatc gtaacgcgct ggctctttta ccacttttgt taaggtgtta cgcaaatcac
     2881 ttttccgcgt caataaattt cgtttctcca taccaatcat tcgatccttt tcataagaaa
     2941 aacgttctaa ctcatgtaca taattggtct tcattgcgtc taatgttgct tgtcttctct
     3001 tacctttcgg tacgatataa ctcacaccat tttcaacctc cgtgtaaggt ttacgcgtat
     3061 tttctttttc aacaactgca aaatgaatat ggatatg
//
LOCUS       DACECB010000124         3070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECB010000124 DACECB010000000
VERSION     DACECB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.133"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(334..696)
                     /locus_tag="IWO04_002738"
     CDS             complement(334..696)
                     /locus_tag="IWO04_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214290.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="IWO04_002739"
     CDS             complement(770..1150)
                     /locus_tag="IWO04_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0214291.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="IWO04_002740"
     CDS             1232..2407
                     /locus_tag="IWO04_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0214292.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="IWO04_002741"
     CDS             complement(2484..3068)
                     /locus_tag="IWO04_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0214293.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat
//
LOCUS       DACECB010000125         2965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECB010000125 DACECB010000000
VERSION     DACECB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.134"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            147..1235
                     /locus_tag="IWO04_002742"
     CDS             147..1235
                     /locus_tag="IWO04_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0214294.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQ
                     LKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1246..>2965
                     /gene="topB"
                     /locus_tag="IWO04_002743"
     CDS             1246..>2965
                     /gene="topB"
                     /locus_tag="IWO04_002743"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0214295.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAK"
BASE COUNT         1085 a          526 c          657 g          697 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ctgaactaaa gccgaaacat gaacggcaaa
      241 attttttagt tggagacgac cgtttagatc atgcggttgc ttttttatgg aaagatccac
      301 agacaggaga gacagtaggg gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg
      361 gtaaacgggg aacgtataaa cacattgaca agaatcctac gccaaatcac ggatttaatt
      421 taaaaatagg cgatccgaag catttaaaat tctttgaaag tagtattgat ctgttaagtt
      481 acgctgcatt gaatcgggag aaactacaag atgcctggtt agtttcgatg gacgggttaa
      541 aacatcatgt aatcagtcat tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa
      601 catttcctca atcgattgaa atatgtgtgg acaatgatcg tgcaggacat attttttatg
      661 aaaaagaaca gatgaaggga atcgttgatc cttttaccaa taagaaaatc cgttgtgagc
      721 gaggaattcc aaatgattgg caagtaccta aagaatacaa agctacttat gaagcagtcg
      781 caaaagaaat gagtgttgaa cctgaagcaa ttatggccat acacaaaact gaaactaatt
      841 tacagctgac aaatcaattg gtgtctgctc atgatgttca atctacgttt ggaaagatgt
      901 tggcgaaggg cgaaccagta gaaacaattg atttgaaaga agcatgtacc acggtcgcga
      961 aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa ctttgatcgt ttttatagta
     1021 gaaaagccaa cataaaagac gttaatgcag gaatccttct ttcctataaa gcagaacaat
     1081 actacaaagg ctataaaaag catgaacatg aatttgtacc tgaagtgaaa aaggattgga
     1141 atgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt
     1201 tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt
     1261 ttagcagaaa aaccgtcaca agcgaaggct tatgccgata gcttttctaa agctacccga
     1321 aaagatgggt attttgagat ccaagatcga ttgtttacag gagaaactgt gatcacgtat
     1381 ggttttggtc atttagtcga gttagattcg cccgatatgt acgatgaaaa atggaaacaa
     1441 tgggcattag aacatttgcc tattttcccc aatcaatacc actatcatgt tcctaaagat
     1501 aagaaaaagc agtttagtgt cgtcaaacga cagctgcagt cagctgatac gattgtgatc
     1561 gcgactgata gcgatcgtga aggggagctg attgcctggt caatcattca acaagcaggc
     1621 gccaatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa agaagccatt
     1681 tatcagggat tccaacagtt acgagacgca gaagagacct atccaaaatt tgaagaagta
     1741 caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact gtatagttta
     1801 ctcttgcagc aaaaaggcat accaggaagt ttttctttag gtagagtcca aaccccaaca
     1861 ctctatatga tttatcaatt gcaggaaaag attagaaact tccaaaaaga accatatttt
     1921 gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat
     1981 gaaacccaag cgacacaaga agcatttgaa gcctatttaa aagaaaaagg tgttcaatta
     2041 ggaaaacagc caggtacgat ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca
     2101 cgtctttttt ctttatcgag ccttcagtct aaaatgaatc aactcatgaa agctagtgcc
     2161 aaagatactt tggaagccat gcaagggttg tatgaaggaa aatatctaag ctatccgcga
     2221 acggataccc cttatattac ggaaggggaa tatgcgtatc tgttggatca cttagacgaa
     2281 tacaagcatt ttttaaaagc cgaagcgatt ccgactccta ttcatacacc gaatagtcgg
     2341 tatgtgaata ataagaaagt ccaggaacat tacgcgatca ttcctacaaa aacggttatg
     2401 acagcagcgg cttttgaaca actaagtcca ctgcagcagg ctatttatga acaagtgtta
     2461 aaaacaacgg tggccatgtt tgcggaaaag tatacctatg aagaaacaac gatcttgacg
     2521 caagttcagc agcttcagtt aaaggcaatc gaaaaggtgc cgctagatct aggttggaag
     2581 aagttatttg gaaaagagag cgacggaaaa gagaaggaag aagagccgct acttcctaaa
     2641 gtgacaaaag gagaaatggt gacggtggat ctacaagttc ttgaaaaaga aaccaagcca
     2701 ccacaacctt ataccgaagg tacgttgatc actgcaatga aaacagctgg gaaaaccgtg
     2761 gatagtgaag aagcgcaatc catattgaaa gaagtggaag gaatcggtac agaagcgaca
     2821 cgggcgaata ttattgaaac cttgaaacaa aaggaatata tcaaggtaga gaaaaataag
     2881 ctcgtggtga cgaataaagg gattttgctt tgccaagccg tggaaaaaga gccgctgctt
     2941 acgagcgccg aaatgaccgc caaat
//
LOCUS       DACECB010000126         2786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECB010000126 DACECB010000000
VERSION     DACECB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.135"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..656
                     /locus_tag="IWO04_002744"
     CDS             54..656
                     /locus_tag="IWO04_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214296.1"
                     /translation="MLKLISYAKKANSLSLVDNLDNLLPDKLIYDENEDNSQKQIIIF
                     ELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDD
                     LVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYD
                     PLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
     gene            complement(653..1276)
                     /locus_tag="IWO04_002745"
     CDS             complement(653..1276)
                     /locus_tag="IWO04_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214297.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            1594..1932
                     /locus_tag="IWO04_002746"
     CDS             1594..1932
                     /locus_tag="IWO04_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214298.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            2024..2765
                     /locus_tag="IWO04_002747"
                     /pseudo
     CDS             2024..2765
                     /locus_tag="IWO04_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a          427 c          483 g          871 t
ORIGIN      
        1 aatttttaga agaattatct ggagaagaat atagattatt cctaaataat aatattttaa
       61 aattaatatc ttatgccaag aaagctaata gcctatcttt agtagataac ttagataatc
      121 ttttacccga taaacttatt tatgatgaga atgaagataa ttcacaaaaa caaataatta
      181 tatttgaatt gtatttaaag tggcttagca gcctgtcagt gtcaccagaa agcaatgatg
      241 atgataaata ttttgcgaac aaactgtttc aatttatatt tccagactat tcttcaatgc
      301 cattttttac attaataaat tttatgctgt atgttaaaga acacttagga tatgatgatt
      361 tagtagaaat gataaaggca tatgataaag aaccttatcc attcaaagga atcaatactg
      421 gttcagatat ggctatgatt tcaggcgaaa ctgtagaaga aattctagct aattacaaag
      481 aacaaaatat aaaaactctg gaattaatta aaaagaatat aaaatactat gatcctttac
      541 tagcgaataa agaaaatatt gataagttat taaatgcaat tgctgattct gatgttagat
      601 ctcctagaat tgatgagtta aaagaagaaa ttttaatgta taaagaactt ttttaattca
      661 tttgattact gttatactca atatcattct taattctgta aatagtttgt ctagtaatat
      721 tgtagttttt agcaattttt gaaatagcta tgccctcatt taaatcctta acgatgtttt
      781 cataaactaa acgtcgttga ggattttttg tatctgcact atagagtttt ggacgacctt
      841 tatagacgcc atttgctttt gcaattttaa tgacttgcgc ctggcgtcgt ttagattcgg
      901 tacgctcttg ctctgcaatc atggccaaaa tttggatgat taaatcttta atgaatttat
      961 ccaaatgagg attgccaatt gcttcagcca tgatcggtag actggtgatg atcagctgca
     1021 cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt cttccaagtc
     1081 ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa gcttcctgga
     1141 aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt ttttctgcgc
     1201 cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac gaaactcgaa
     1261 tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata cactcaaaat
     1321 caacactaat ttttttatac actataaagc tgtcattttt ctagataaat ggcataaatt
     1381 ttcagcgcta agtgacaaaa gcaactaatt tttcatgata gatttttaaa tatctcaatc
     1441 tatctccaaa attagttgct tttgtagcta tagttgacta tgaatatgac aaataattag
     1501 aaaaaactgt gccaattaaa tatcaaaatg acaaattgat gaatcaaatt caaattaagg
     1561 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
     1621 aggcgtttga ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga
     1681 aaaaaaatta aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc
     1741 aaaggtcggc aactgaaatt taaaaaaaat gatccacgtt taaagcatgc tttcgatttg
     1801 tttttgaatg gtttatccga taaagaagtt gaagaacaaa ctgggatcaa tcgccgaacg
     1861 tttagaaggt atcgagcaag atataacgtg acagtagatc aaagaaaaaa caatgaaaag
     1921 agggatagtt aatgagtacg gttattttag ctgaaaaaca agccaggcat tagcctacgc
     1981 aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag acccactatt
     2041 tacagatgaa acgtttatca cctttgattt tgggcattta gtggaattag cagaaccagg
     2101 tcattatgac gaaaagtggc aaaattggaa acttgaatct ttgccgattt ttcctgatcg
     2161 atacgatttt gaagttgcaa aagataaggg aaagcagttt aaaattgttg cagaacttct
     2221 caaaaaggca aatacaatta ttgttgcaac agatagcgac agagaaggtg aaaatatcgc
     2281 ctggtcgatt atccataaag caaatgcctt ttcaaaagat aaaacattta aaagactatg
     2341 gatcaatagc ttagaaaaag atgtgatccg aagcggtttt caaaatttgc aaccaggaat
     2401 gaattactat cccttttatc aagaagcgca aacacgccaa attgccgatt ggttgatcgg
     2461 catgaacgca agccctttgt atacgttaaa tttacaacag aagggcgtac aaggtacatt
     2521 ttcactagga cgtgttcaaa cgcccacctt ataccttatt tttcagcgcc aggaagctag
     2581 tataaaagta aaacaagggt catttaaggg cgttctaagc cccacacagc gttttaaaac
     2641 ccaagaggag cttttttgct tttgtttctt ctaaacaagc taaaataggc aatcaagagg
     2701 ggataattgc tgatgttcaa accaaagaga tggataggct acaattaact agacagaaaa
     2761 attaaggtgt gtagactaga agaaaa
//
LOCUS       DACECB010000127         2601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECB010000127 DACECB010000000
VERSION     DACECB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.136"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..484
                     /locus_tag="IWO04_002748"
     CDS             47..484
                     /locus_tag="IWO04_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214299.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            477..1172
                     /locus_tag="IWO04_002749"
     CDS             477..1172
                     /locus_tag="IWO04_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214300.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            1553..1732
                     /locus_tag="IWO04_002750"
                     /pseudo
     CDS             1553..1732
                     /locus_tag="IWO04_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1794..1978
                     /locus_tag="IWO04_002751"
                     /pseudo
     CDS             1794..1978
                     /locus_tag="IWO04_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            2012..2443
                     /locus_tag="IWO04_002752"
     CDS             2012..2443
                     /locus_tag="IWO04_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0214301.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          937 a          405 c          535 g          724 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
       61 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      121 attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      181 tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct actatcgaac
      241 gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac tatgcttgga
      301 agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag aactaacgta
      361 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tggactactt
      421 agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca acgaaaatga
      481 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      541 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      601 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
      661 ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag
      721 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
      781 gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat tactcagaat
      841 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
      901 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
      961 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
     1021 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
     1081 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
     1141 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
     1201 gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
     1261 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
     1321 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
     1381 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
     1441 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
     1501 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
     1561 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
     1621 gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
     1681 attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt gatcgcactt
     1741 tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc acaatgccac
     1801 ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc gaaggcaaga
     1861 gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg ttcatgccaa
     1921 tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat tgaaataatc
     1981 tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag caaaaaccta
     2041 tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc ctgatgttca
     2101 aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta tggcacaaga
     2161 ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc cagaaccaac
     2221 accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc gtgtagatgt
     2281 tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc ctcaatgggc
     2341 ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag aatcaattgc
     2401 agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa aagggtcaga
     2461 aacttaaaaa tctttggctt ctttttttac tacaaagatg gcttctttaa attatttgcc
     2521 tgattgattt aaaaaaattg cgcaaagact acgagctaat taagttgctc gtggtctttt
     2581 tttatgaaaa ctttttagat c
//
LOCUS       DACECB010000128         2584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.137, whole genome shotgun sequence.
ACCESSION   DACECB010000128 DACECB010000000
VERSION     DACECB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.137"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..666
                     /locus_tag="IWO04_002753"
     CDS             1..666
                     /locus_tag="IWO04_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214302.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            710..979
                     /locus_tag="IWO04_002754"
     CDS             710..979
                     /locus_tag="IWO04_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214303.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1211..1507
                     /locus_tag="IWO04_002755"
     CDS             1211..1507
                     /locus_tag="IWO04_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214304.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1509..1931
                     /locus_tag="IWO04_002756"
     CDS             1509..1931
                     /locus_tag="IWO04_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214305.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1950..>2584
                     /locus_tag="IWO04_002757"
     CDS             1950..>2584
                     /locus_tag="IWO04_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214306.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEG"
BASE COUNT          990 a          362 c          493 g          739 t
ORIGIN      
        1 gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca
       61 gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt
      121 atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta
      181 ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg
      241 ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa
      301 gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc
      361 gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat
      421 tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta
      481 accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccaa
      541 agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa
      601 ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg
      661 atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct
      721 ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt
      781 caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca
      841 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca
      901 acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt
      961 caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa
     1021 acgtgacccc acttttaaaa aaagtggttg gataggctgt gcctatcacg tttctgaaaa
     1081 aaataaatga ccccaaaata ccaatatata ggatagtaca aggatagcaa aggatagtaa
     1141 aaggatagag gtaggatagc gaaaaggata ggatcctttt cactatccta tattatattg
     1201 gaggatattt atgaatgtta aaattagaga tttagatcca aaattcattt ctgaaattga
     1261 tcgaagatgc gttgaactaa gtaatcgtac aggaaataaa tggagtcgta atgattattt
     1321 aaagctactc gttgaaaatg attttgatcg tcctttgatg gaatacaaac aagaaaagtt
     1381 tgaccagtta ttagaaaagt tttcagaaat tcaaagttat aacacaaaga tattagaaga
     1441 atatgttagc caaaataatc gtattataga aatcttgatt gagcaaaata gaggaagtga
     1501 actttgatat ggcaaaggtt atagacaaac acatttactt tcaccaaaca catatcgatg
     1561 tattggatga aactatttct actcatgcag aggttaaaaa ctatagtgaa gctgttcgct
     1621 ttttatgtat gaactatgat ccagaagtgg agaaaaaagt aaattccaat caagcaaagt
     1681 tgaatggaat gagtaaagaa atttctattt taaccgaaat ggtttcagaa tttgcggatc
     1741 ttcatttgaa agatacagga atattaattg gtaaaaattc aactgtttat caagaagcaa
     1801 aagaacgagt ggaaggaaaa attaaatcca atcaaacaaa aaaatattcc cctcagttga
     1861 agaaacctgt gaatgaatct gttcatgcca gagtagatcc atttgatcca acgaagattc
     1921 taaaaagata aaaatgaagg aggataataa tgactcaatc gccttcagtt attctcactt
     1981 gtcaatttac tttgccgaat aaaaagtcat tttctactta tattgactat attacaaggg
     2041 aacaggctct tgaagaaaag gaacgacgta cgcccgaaga agaacaggaa cttcaacgaa
     2101 tcaagaatgc tttagaggag ttttatattc ctgaaggaga aactttttcg gagagtaaga
     2161 cgggtaaaga attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca
     2221 attttgacaa gttagaggaa caagatttta ccaattatct ttcttatatg acacgtcact
     2281 atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca aaaagaatac caacgcttga
     2341 accaagcaat gaaaaaatat caaacagaac cccataaaaa agcttcagga aatcttcctg
     2401 gagttttttc tatagcttca gacgaagtga aaggaagtga tctgaaggag attaagcaaa
     2461 tcttccaaag aggacaacaa aaaggctcaa tcatttatca agatgttgtt tctcatgata
     2521 attcgtatct tgaaaaaata ggattgtacg atcccaaaac agatacttta gatgaagaag
     2581 ggtt
//
LOCUS       DACECB010000129         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECB010000129 DACECB010000000
VERSION     DACECB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.138"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO04_002758"
     CDS             207..2069
                     /locus_tag="IWO04_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0214307.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWO04_002759"
     CDS             2069..>2398
                     /locus_tag="IWO04_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0214308.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACECB010000130         2395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECB010000130 DACECB010000000
VERSION     DACECB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.139"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWO04_002760"
     CDS             316..570
                     /locus_tag="IWO04_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0214309.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWO04_002761"
     CDS             571..888
                     /locus_tag="IWO04_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0214310.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IWO04_002762"
     CDS             1447..2382
                     /locus_tag="IWO04_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214311.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DACECB010000131         2386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECB010000131 DACECB010000000
VERSION     DACECB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.140"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..557)
                     /locus_tag="IWO04_002763"
     CDS             complement(129..557)
                     /locus_tag="IWO04_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214312.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(585..1223)
                     /locus_tag="IWO04_002764"
     CDS             complement(585..1223)
                     /locus_tag="IWO04_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0214313.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1227..1997)
                     /locus_tag="IWO04_002765"
     CDS             complement(1227..1997)
                     /locus_tag="IWO04_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0214314.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT          755 a          443 c          354 g          834 t
ORIGIN      
        1 atttttttgt tgtttttatt atgatatatt tttatacaac taaaaatgaa tatattaaaa
       61 ctaccaaaaa taacttttaa cgctgtttca ttatatgttt tttctatttt atataaactt
      121 ttggatactt aataccaacc ggttggattg taccaacatg agaatgtgtc agttctattt
      181 cctgtcgcac ttgcataggc ccatactcct tttgttgcat ttgctgatcg aatagagttt
      241 ccaactttcg cttttgcacc gtgacgatta tagacacgac tatagtggtt agaatgacct
      301 gtttttctcg tacaagttgt atcactataa taaacccatc ttccagagct atgattaatt
      361 gctgttctag aaatttcccg gtctactggt aaatttgact taataatatt acctttagca
      421 tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga tttcaccgtg aactgaattt
      481 gcttccactt cagatgacat agcgtttcct ccaacaaaac ctatccctag tgcacaaata
      541 ataactgctt ttttcataaa acgacatcct ttcctttttg atttttaaag catgatcttc
      601 ttgtttccta ttttggtcag atatggatca tgcgtgacta cgataactaa cgtaccttgg
      661 gaagcttgtt gcgataagat ttcaactact ttatccttat ttcttttatc caatgaccca
      721 gtcggttcat cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt
      781 ctttgcttct caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt
      841 acttttttca atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac
      901 ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga
      961 aaaagaaaag atatggtatt tctttttatc aagcgcttct ctctcccagt taattgtttc
     1021 gtctcttgat cgaacaaata gaattgtcct gaactgaact gatcattcaa tcctaatata
     1081 ttcaataacg tacttttacc tgctccactt tctccaacaa cagtcacaag atccccagca
     1141 tccgcagtga aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct
     1201 tttatcacta tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc
     1261 ttttctgtag acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa
     1321 atattaaaaa gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt
     1381 ccccatttga tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca
     1441 cttgaacgaa agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca
     1501 tagctcaaca agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat
     1561 gatctgctta acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc
     1621 aagttatcac gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag
     1681 cctatcttga tctttaacgg tggatagatt tctccgctga gaatatttct ttccaaatac
     1741 atactgtttt tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa
     1801 ggaataaatg tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca
     1861 ctgatttctt gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta
     1921 tatcgttcag ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt
     1981 ccagagacat caatcaaagg aaatttctta agataattca gatttatttt tatccctctg
     2041 ccaaactgtt cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt
     2101 ttattcaaag tttcatataa ttcctgatct gctgctgatg caaaacgatt ggaataaata
     2161 aaatccacgt ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg
     2221 atttcttttg gtggttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct
     2281 aattcgacat tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg
     2341 tattggatat agttgaacgc ttctcttaac gaagcgattt tttcaa
//
LOCUS       DACECB010000132         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.141, whole genome shotgun sequence.
ACCESSION   DACECB010000132 DACECB010000000
VERSION     DACECB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.141"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1919)
                     /locus_tag="IWO04_002766"
     CDS             complement(<1..1919)
                     /locus_tag="IWO04_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0214315.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDK"
     gene            complement(1926..2222)
                     /locus_tag="IWO04_002767"
     CDS             complement(1926..2222)
                     /locus_tag="IWO04_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214316.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          667 a          467 c          368 g          869 t
ORIGIN      
        1 tgtttatctg taatccctaa tctttgagca ggtgtcaaaa cctttttatc cgctgattta
       61 aaagggcgac aaaagttgta gtaagttctt agaattgtaa tcgcaaattg agcatacttc
      121 ggattgaagt tagcgtaaat atagctcttc ttatcaccac gagctgttgt taatggtctt
      181 tcaagagatg atatataacg tcttatctgt tgaataaagc tattggtcga atggtcatta
      241 acatttaata gcatctttgc gatttcgttt ggttccagag cagataaatc cgttctacaa
      301 tcaactgaat aaaatccttt atccttagtg gctaatggat gtttgatagg gttatcggca
      361 tatttgcgat atctttctcc atctttacta atagcctctt cgtggaattg atgacttgtt
      421 agtaattcag ttagataatg agatgccaaa gtccgtagaa actttgtttt gaatcctctg
      481 atatctcccc aatcgagcag ttctgctttt gcttgtccga attcttttaa gcattgtttt
      541 ctgcttttcg tcttgttaac ttggcaaata aaatgatgtg catcgctcaa ccttaattct
      601 ttcgtgaata ctctataaaa ggcgtttcta atagagaagt catcatcact aatcattcgc
      661 cattcgtcgg cattaaccat ttgtttaatt aaccaatagt gagcagttgt agtataagga
      721 gcatcaacat ttaaaccatc gacaaatcta ctcctattag taattttccc taattcgtga
      781 agataagcat tcctattttc gctatcgtta gcggatggtt cttgtggata ataagaccaa
      841 tctaacctat catttttacg actaaaagta ttgagatggt cttctttgaa ttttcttgta
      901 tcttcattta attcatccat tgaaatattc caatcatagg caacatcaga acggaacaca
      961 tatcttgata agacctcagc agatacaact atatacgttt gcatattaag gtcttcaaac
     1021 cccgtatatt tctttgaacc ttgtcctttc ttacggacgt tatttaggta gtagtgcatc
     1081 ttgtccgtat taagccacat ctcgttaaat ttctttgttt gaaggggttg tgtctcatat
     1141 cgttctaaaa actccaaaca acgcctatac agccactcca gcttatgata gtaggttcca
     1201 actccaattc ctaaaatatc acaagtacga ctgactgaaa cccttccaac aaccatctta
     1261 gcgaacattg gaagaatggt gttcttttgt tggtgataag tagtggtttc ttctcttttt
     1321 ggtagaacat tagtgaactt cttacacgcc ttacattgat agcgttgaga tttgccccta
     1381 cttttccccc ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc ttcttccgag
     1441 cagccttctt tatgaaattg ataatctggc tcaacatctt ggatgctatt gatttctata
     1501 aggcgtttaa tttcttcaac aacagaccaa ttagataaag gggttactgt acagttttgc
     1561 gatacacctc tgccaatagg attgtcattg cagtacatac ctttatgacc tttatctttg
     1621 gaactaccag tcattttata tcgacttggt ttccctttta catctttgaa tttgtgctgt
     1681 tctttcccaa aattacaaca aaacggattg atgcagtaat tatattgaat gggatattca
     1741 gaaccgttcc atttaaaaga cgttggcaag aacaacattt tgttatacct tgttgcaaat
     1801 cgttttggag ttaataaatc atattgttta gccctatctt ttaattcttc gtttgaaatc
     1861 ggaattttaa catctacaat ttcatcttcc ttcgtggcta atcttttaag cttggtcatt
     1921 gtctatcaac ccactctatc tttgataccc attttttcaa caatccgctt attacttcgc
     1981 ttgttggcaa tccattcaat aaacttctta tcgtctaaaa tgttcaataa gaattcatcc
     2041 actgcttcgc tttcagtacg ctctgcatat tcagcgtatt gcttaactat cacccttact
     2101 tgctctgata tttcccaatc cacagactta gcgttcttat tttttggctc aagaaattgc
     2161 ataggaaaat gcaccccttt ttgcttagta aattgtaacc attttaacac accaatttgc
     2221 attcctacaa taattatttc acgttcgcat ttttatatac attttaactt acattttgtg
     2281 gtgtaaatta acaacaaacg ttgatttaac aataaaaaac gagtatcaaa ttcagttgca
     2341 atttgatact catttaggtt tacattttca c
//
LOCUS       DACECB010000133         2342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.142, whole genome shotgun sequence.
ACCESSION   DACECB010000133 DACECB010000000
VERSION     DACECB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.142"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            204..1226
                     /locus_tag="IWO04_002768"
     CDS             204..1226
                     /locus_tag="IWO04_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0214317.1"
                     /translation="MIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1237..>2342
                     /locus_tag="IWO04_002769"
     CDS             1237..>2342
                     /locus_tag="IWO04_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0214318.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVN"
BASE COUNT          845 a          420 c          494 g          583 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa
      241 atttcttagt tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac
      301 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
      361 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
      421 taaagatcgg tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct
      481 atgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
      541 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
      601 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
      661 aaaaagaaca attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac
      721 gaggaatcgc taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg
      781 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
      841 tgcaattaac gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc
      901 tttctgttaa cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa
      961 aagaattaaa agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag
     1021 aaaaagggga tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag
     1081 gctataaaaa tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat
     1141 tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac
     1201 aaggcagaca acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa
     1261 aagccgtcac aagcgaaagc ttatgccgat agcttctcta aagccactcg aaaagacggg
     1321 tattttgaaa tccaagatcg attgtttcct ggtgaaactg tgatcacgta tggttttggt
     1381 catttagtcg aattagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     1441 gaacatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     1501 caattcaagg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     1561 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagtagg cgccgatcaa
     1621 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     1681 ttccagcagt tacgagacgc aaaagagacc tatccaaaat ttgaagaagc acaagcacgt
     1741 cagatcgccg attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag
     1801 caaaaaggca taccaggaag cttttcctta ggcagagtcc aaaccccaac gctttacatg
     1861 atttatcaat tgcaggaaaa gattagaaat ttcaagaaag aaccttattt tgaaggaaaa
     1921 tccgaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa
     1981 gcgacgcaag aagcatttga agattattta aaagaaaagg gggttcaatt gggaaaacag
     2041 ccaggcatga ttcgccaagt tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     2101 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagacact
     2161 ttggaagcca tgcaaggctt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     2221 ccttatatta cggaagggga gtatgcgtat ttgttggatc atttagatga atacaagcat
     2281 tttttaaagg ccgaatcaat cccaacccct attcatacgc cgaatagtcg gtatgtgaat
     2341 aa
//
LOCUS       DACECB010000134         2220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECB010000134 DACECB010000000
VERSION     DACECB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.144"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IWO04_002770"
     CDS             complement(51..1598)
                     /locus_tag="IWO04_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0214319.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO04_002771"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO04_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0214320.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DACECB010000135         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECB010000135 DACECB010000000
VERSION     DACECB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.145"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            493..714
                     /locus_tag="IWO04_002772"
     CDS             493..714
                     /locus_tag="IWO04_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214321.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IWO04_002773"
     CDS             715..972
                     /locus_tag="IWO04_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214322.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IWO04_002774"
     CDS             complement(1108..1710)
                     /locus_tag="IWO04_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0214323.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IWO04_002775"
     CDS             complement(1777..1932)
                     /locus_tag="IWO04_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0214324.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACECB010000136         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECB010000136 DACECB010000000
VERSION     DACECB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.146"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWO04_002776"
     CDS             287..694
                     /locus_tag="IWO04_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214325.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWO04_002777"
     CDS             708..1109
                     /locus_tag="IWO04_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214326.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWO04_002778"
     CDS             1111..1482
                     /locus_tag="IWO04_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214327.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IWO04_002779"
     CDS             complement(1518..1820)
                     /locus_tag="IWO04_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214328.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          764 a          270 c          337 g          649 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt
//
LOCUS       DACECB010000137         2015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECB010000137 DACECB010000000
VERSION     DACECB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.147"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWO04_002780"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWO04_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0214329.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWO04_002781"
     CDS             852..1040
                     /locus_tag="IWO04_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0214330.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWO04_002782"
     CDS             1053..1610
                     /locus_tag="IWO04_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0214331.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWO04_002783"
     CDS             1642..1884
                     /locus_tag="IWO04_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0214332.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          737 a          309 c          407 g          562 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaat
//
LOCUS       DACECB010000138         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECB010000138 DACECB010000000
VERSION     DACECB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.148"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IWO04_002784"
     CDS             <1..376
                     /locus_tag="IWO04_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0214333.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="IWO04_002785"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="IWO04_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0214334.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="IWO04_002786"
     CDS             1857..>2001
                     /locus_tag="IWO04_002786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0214335.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DACECB010000139         1979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECB010000139 DACECB010000000
VERSION     DACECB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.149"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(325..528)
                     /locus_tag="IWO04_002787"
     CDS             complement(325..528)
                     /locus_tag="IWO04_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214336.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="IWO04_002788"
     CDS             complement(728..1744)
                     /locus_tag="IWO04_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0214337.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DACECB010000140         1954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.150, whole genome shotgun sequence.
ACCESSION   DACECB010000140 DACECB010000000
VERSION     DACECB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.150"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWO04_002789"
     CDS             156..1862
                     /locus_tag="IWO04_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0214338.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DACECB010000141         1751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.151, whole genome shotgun sequence.
ACCESSION   DACECB010000141 DACECB010000000
VERSION     DACECB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.151"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(125..196)
                     /locus_tag="IWO04_002790"
     tRNA            complement(125..196)
                     /locus_tag="IWO04_002790"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Glu,seq:ttc)
     gene            complement(208..281)
                     /locus_tag="IWO04_002791"
     tRNA            complement(208..281)
                     /locus_tag="IWO04_002791"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Ile,seq:gat)
     gene            complement(294..364)
                     /locus_tag="IWO04_002792"
     tRNA            complement(294..364)
                     /locus_tag="IWO04_002792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Gly,seq:tcc)
     gene            complement(369..441)
                     /locus_tag="IWO04_002793"
     tRNA            complement(369..441)
                     /locus_tag="IWO04_002793"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Phe,seq:gaa)
     gene            complement(445..517)
                     /locus_tag="IWO04_002794"
     tRNA            complement(445..517)
                     /locus_tag="IWO04_002794"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Asp,seq:gtc)
     gene            complement(536..609)
                     /locus_tag="IWO04_002795"
     tRNA            complement(536..609)
                     /locus_tag="IWO04_002795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Met,seq:cat)
     gene            complement(622..711)
                     /locus_tag="IWO04_002796"
     tRNA            complement(622..711)
                     /locus_tag="IWO04_002796"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(673..675),aa:Ser,seq:tga)
     gene            complement(734..805)
                     /locus_tag="IWO04_002797"
     tRNA            complement(734..805)
                     /locus_tag="IWO04_002797"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(770..772),aa:Glu,seq:ttc)
     gene            complement(827..914)
                     /locus_tag="IWO04_002798"
     tRNA            complement(827..914)
                     /locus_tag="IWO04_002798"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Ser,seq:gct)
     gene            complement(939..1012)
                     /locus_tag="IWO04_002799"
     tRNA            complement(939..1012)
                     /locus_tag="IWO04_002799"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(976..978),aa:Ile,seq:gat)
     gene            complement(1026..1096)
                     /locus_tag="IWO04_002800"
     tRNA            complement(1026..1096)
                     /locus_tag="IWO04_002800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1062..1064),aa:Gly,seq:tcc)
     gene            complement(1098..1173)
                     /locus_tag="IWO04_002801"
     tRNA            complement(1098..1173)
                     /locus_tag="IWO04_002801"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Asp,seq:gtc)
     gene            complement(1192..1265)
                     /locus_tag="IWO04_002802"
     tRNA            complement(1192..1265)
                     /locus_tag="IWO04_002802"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1229..1231),aa:Met,seq:cat)
     gene            complement(1278..1367)
                     /locus_tag="IWO04_002803"
     tRNA            complement(1278..1367)
                     /locus_tag="IWO04_002803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1329..1331),aa:Ser,seq:tga)
     gene            complement(1391..1464)
                     /locus_tag="IWO04_002804"
     tRNA            complement(1391..1464)
                     /locus_tag="IWO04_002804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1428..1430),aa:Ile,seq:cat)
     gene            complement(1478..1551)
                     /locus_tag="IWO04_002805"
     tRNA            complement(1478..1551)
                     /locus_tag="IWO04_002805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1515..1517),aa:Met,seq:cat)
     gene            complement(1579..1651)
                     /locus_tag="IWO04_002806"
     tRNA            complement(1579..1651)
                     /locus_tag="IWO04_002806"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1616..1618),aa:Ala,seq:tgc)
     gene            complement(1671..1744)
                     /locus_tag="IWO04_002807"
     tRNA            complement(1671..1744)
                     /locus_tag="IWO04_002807"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Pro,seq:tgg)
BASE COUNT          494 a          471 c          401 g          385 t
ORIGIN      
        1 aaatatttta cattagaact gtccaaaaaa gcttagcaca atcatccata aagataactc
       61 tgctaagctt tttaaaatca ttctttcaga taaaaagaaa aagatactca aaaagtatct
      121 tccatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc
      181 gcttgaccaa cgggccaata aaataaatgg gcctaaatgg actcgaacca tcgacctcac
      241 gcttatcagg cgtgcgctct aaccagctga gctataggcc cataaaaact aaaagcgggt
      301 gacgagaatc gaactcgcga caacagcttg gaaggctgtg gttttaccac taaactacac
      361 ccgcataatg gcgcgggaca gaatcgaact gccgacacat ggagcttcaa tccattgctc
      421 taccaactga gctaccgagc caaacggttc cgacgggaat cgaacccgcg atctcctgcg
      481 tgacaggcag gcatgttaac ccctacacca cggaaccaat gtttttagtt tttaattgcg
      541 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
      601 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      661 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catgtaaaac
      721 tatattaaat taatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt
      781 gtcttaaccg cttgaccaac gggccaggta aaacaaaata ttgaaacgga gaaggaggga
      841 tttgaaccct cgcgccggtt tcccgaccta cacccttagc aggggcgcct cttcagccac
      901 ttgagtactt ccccaaaacc aataagattt tgtactaatg ggcctaaatg gactcgaacc
      961 atcgacctca cgcttatcag gcgtgcgctc taaccagctg agctataggc ccataaaaaa
     1021 ctaaaagcgg gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tggttttacc
     1081 actaaactac acccgcatgg cggttccgac gggaatcgaa cccgcgatct cctgcgtgac
     1141 aggcaggcat gttaacccct acaccacgga accagtgttt ttagttttta attgcgggag
     1201 caggatttga acctacgacc ttcgggttat gagcccgacg agctaccaga ctgctccatc
     1261 ccgcgataat atgaaaaagg aggataaggg attcgaaccc ttgcacggtt ttacccgcct
     1321 gacggttttc aagaccgttc ccttcagccg gacttgggta atcctccata tgaaacttac
     1381 aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg agccgtctgc
     1441 tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg gatcgaaccc
     1501 ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc gaaaaagata
     1561 ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc tgcgtgcaaa
     1621 gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta tcgggaagac
     1681 aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc tgagctactt
     1741 cccgtttaat t
//
LOCUS       DACECB010000142         1719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.152, whole genome shotgun sequence.
ACCESSION   DACECB010000142 DACECB010000000
VERSION     DACECB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.152"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..375
                     /locus_tag="IWO04_002808"
     CDS             10..375
                     /locus_tag="IWO04_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0214339.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IWO04_002809"
     CDS             376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IWO04_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ0214340.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY"
BASE COUNT          711 a          130 c          296 g          582 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
     1501 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
     1561 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
     1621 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
     1681 aaagcaaaag aatatcaaga tatagttgaa gaatattat
//
LOCUS       DACECB010000143         1569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.154, whole genome shotgun sequence.
ACCESSION   DACECB010000143 DACECB010000000
VERSION     DACECB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.154"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..635
                     /gene="mobC"
                     /locus_tag="IWO04_002810"
     CDS             24..635
                     /gene="mobC"
                     /locus_tag="IWO04_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0214341.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            620..>1569
                     /locus_tag="IWO04_002811"
     CDS             620..>1569
                     /locus_tag="IWO04_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0214342.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNY"
BASE COUNT          622 a          203 c          296 g          448 t
ORIGIN      
        1 aaaaaaatgt gaggggataa tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca
       61 aacatagtga ggccttaaat tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga
      121 ttgcaaaaca aaggggaatt tctagagcag acgtttgccg tgagcggtta aagggactca
      181 aatatgaacc gccgttgtta gctgaagatg atagggtagt tttaaccaaa cagttaaatc
      241 aaattggtaa caatttgaat caagcggtaa aggcattaaa tatcattgct aaatatttta
      301 gagaagaatc tgaagataaa aaagaaaaga gcctacattt tagttttgat gctagccgtt
      361 acaacatttt taaagatgaa cttgcgaata gcatagcaaa aaagttgatg attagttatc
      421 cagatgacat ttttttggag tccaatcgga gtgccccctc tttttttaaa ttatcagaag
      481 tagaaaaaga atatttcgaa tattggatag aaaagcaaac tagaccgggt acgaaagaag
      541 agtattccca aaaagttgat cggaatttaa ttcactcaat tcagattatt gcaagtttaa
      601 aagatgaggt gaaacaactt tgggagctac tataacatcg aaaggacgca agaaaaattt
      661 atcctcgcta ttacaatatg cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc
      721 aggaataaat tgtagtgatg atagtgagga agcactgcaa gaaatgaatt tagtacgtac
      781 caagatgaat aaatctaaag atatagcaca agcgtttcat gttattcatt cgtttgataa
      841 gagtacttct aaagagttaa gtattgataa aatgcacgaa atagctgtcg aatttgcaga
      901 gaaagctttt ccaaattcgc aaatcattgt tgcaagtcac aatgataaag atcattttca
      961 tagtcatctt gtcattaata atattaacat ggaaacatgt aaaaggttgc gtattgaccc
     1021 taaagattta gaactaatgc gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga
     1081 atcagtgaat aatgagtact atgaaaactt agaaaagaga accaatcttt attcaaacga
     1141 tccttctaag atgaaatatg gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga
     1201 agcaacgttg gaaaaatgcg aagtgaattc ttttgaagat ttttcggatc atttggcaaa
     1261 agaatataac attgaagttt atcaattttc aaaaacaaat aacaaacttg gctatgttct
     1321 ttacaaacaa ggtgtaaatt tctatgagcg tagaggtgag ttaattactt taggaagaga
     1381 taaacaatct agaaaagaaa aaagtcagta tgtcgaacgt acttttagtg caagaaagtt
     1441 agggaaaaga tattcatttg aatctgtttc tgaacaactt gaaaagaatt tagaagaatc
     1501 acatataaaa aattttttta atatggaaag agagcaagtt atgtcgctaa ttagtggagt
     1561 aaattacaa
//
LOCUS       DACECB010000144         1559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.155, whole genome shotgun sequence.
ACCESSION   DACECB010000144 DACECB010000000
VERSION     DACECB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.155"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..209)
                     /locus_tag="IWO04_002812"
     CDS             complement(<1..209)
                     /locus_tag="IWO04_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0214343.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IWO04_002813"
     CDS             complement(308..622)
                     /locus_tag="IWO04_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214344.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IWO04_002814"
     CDS             complement(690..1184)
                     /locus_tag="IWO04_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214345.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="IWO04_002815"
     CDS             complement(1177..1491)
                     /locus_tag="IWO04_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214346.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
BASE COUNT          475 a          289 c          191 g          604 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttaccc
//
LOCUS       DACECB010000145         1531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECB010000145 DACECB010000000
VERSION     DACECB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.156"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1343
                     /locus_tag="IWO04_002816"
     rRNA            <1..1343
                     /locus_tag="IWO04_002816"
                     /product="23S ribosomal RNA"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="IWO04_002817"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="IWO04_002817"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DACECB010000146         1483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.157, whole genome shotgun sequence.
ACCESSION   DACECB010000146 DACECB010000000
VERSION     DACECB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.157"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..485
                     /locus_tag="IWO04_002818"
     CDS             <1..485
                     /locus_tag="IWO04_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0214347.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(692..1093)
                     /locus_tag="IWO04_002819"
     CDS             complement(692..1093)
                     /locus_tag="IWO04_002819"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214348.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1214..1297)
                     /locus_tag="IWO04_002820"
                     /pseudo
     CDS             complement(1214..1297)
                     /locus_tag="IWO04_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1320..>1483
                     /locus_tag="IWO04_002821"
     CDS             1320..>1483
                     /locus_tag="IWO04_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214349.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
BASE COUNT          482 a          277 c          287 g          437 t
ORIGIN      
        1 taaaggaagc gatcgatcaa atcaagacgg ctgtcttgta tcctcataac ggattaccga
       61 ttttattgat gggtgtaagc ggttcaggaa aaaccttttt ggcagaaaaa atttatgaat
      121 acgccaagga acaacaggtg atagaatcaa atgctccatt tatcgcctat aattgtgctc
      181 aatactttaa tttctcgatt aacaagatcg tagaaaattt aacgatcctt gatgcaatga
      241 aggttttgag attggtacaa aaggccgtca aagaactcga aaaactaatg gggatcgaat
      301 tttcaaacaa tcaattgttc ttgctttaca tgcactgctg ctcaatgatc gaacgtattt
      361 tacgcaaaga aagtgtagat gaacaagcag atatcaaaga atatattcaa aaagagggac
      421 ataattatga aatatcaaaa tacccggctt acaaaacgaa gtttgaaagt actgtaataa
      481 agtaagattt aaataagcat aaacaaggta agaaccatag gggctgtgtc aagtccaaaa
      541 tcttgtgtaa aaaggaaatc tcaaagtacg atgttttgcc ttgttgagcc tagctacggt
      601 ccaatatgct ggtaagaagt gtcaagagca aaaagcgaag cgtctcttga tacttctcac
      661 cagtatatta tactattggc ggcttaatgt gctaaatctc gttacgatca aattcaagat
      721 attttctagg tgcagaagtc cattcgtcat ccttttccat caataaagca ccgatgatac
      781 gaataacaga atcacaattt gggaaaattc ggacaacacg ttcacgacgg cgtacttctt
      841 catttagtct ttcaagaaca ttcgttgttc tcaatcgttg acggatattt tctggtaaaa
      901 ccattacttg catggcatct tcgaagccct catccaatat tttacaggca gttgcgactt
      961 tcttgtcttc cccatattta tccataataa gttgtttacg ttctccagcc agctcgatgc
     1021 ttgccgtttt gaagatggct ttaaagtctt ctttgatatc gcttgagatt ttcttggaga
     1081 atttgtcgga aatgttgcga aggagatgtg cttggcatct ctgctagctc gcacaaagaa
     1141 ggtattcttt cacagctttt gtgagcccta tatgcgcatc cgagatgacc atctttactc
     1201 ctgaaagacc gcgttctttc aatccgagag tgtaaaatat tttgtgtaaa tagaaaaaag
     1261 gaagtccctt cagtagaata gagttaccac aacacattca cagaaaagag gacttccata
     1321 tgaacgattt tactacagaa attctaaaga ctctagcgaa taaaggcgat ttgaatgaat
     1381 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
     1441 tcctcgatta cgaaaagtac gatcgcattg gttttaacac ggg
//
LOCUS       DACECB010000147         1466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECB010000147 DACECB010000000
VERSION     DACECB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.158"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWO04_002822"
     CDS             complement(1014..1304)
                     /locus_tag="IWO04_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214350.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          469 a          288 c          205 g          504 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tataga
//
LOCUS       DACECB010000148         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECB010000148 DACECB010000000
VERSION     DACECB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.159"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IWO04_002823"
     CDS             109..1041
                     /locus_tag="IWO04_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0214351.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IWO04_002824"
     CDS             1113..>1457
                     /locus_tag="IWO04_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214352.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACECB010000149         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECB010000149 DACECB010000000
VERSION     DACECB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.160"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..1415
                     /locus_tag="IWO04_002825"
     CDS             75..1415
                     /locus_tag="IWO04_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0214353.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          321 c          284 g          388 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
      961 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     1021 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     1081 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     1141 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     1201 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     1261 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     1321 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     1381 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     1441 ttgaatttct tcacc
//
LOCUS       DACECB010000150         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECB010000150 DACECB010000000
VERSION     DACECB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.161"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO04_002826"
     CDS             complement(150..1337)
                     /locus_tag="IWO04_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0214354.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACECB010000151         1420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECB010000151 DACECB010000000
VERSION     DACECB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.162"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWO04_002827"
     CDS             4..978
                     /locus_tag="IWO04_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0214355.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DACECB010000152         1384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECB010000152 DACECB010000000
VERSION     DACECB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.163"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..493
                     /locus_tag="IWO04_002828"
     CDS             83..493
                     /locus_tag="IWO04_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0214356.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            507..863
                     /locus_tag="IWO04_002829"
     CDS             507..863
                     /locus_tag="IWO04_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0214357.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          505 a          197 c          255 g          427 t
ORIGIN      
        1 tttttatatt ttattagcta ataattggta ttttaaaaga aattactgta gaattggatt
       61 ataattaaaa aggagtgaga aaatgataaa agtatatact tcgcctagct gtacttcctg
      121 tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt
      181 tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg aaggaggagt
      241 tcaagatatt atttccactc ggtcgaaaat ttacgaaaag ctgggcatag attttaacga
      301 attaacttta aagcaaatga tcgtcttatt caaagaatat ccttcattat tgagacgacc
      361 tattttgatt gatgaaaaaa gaatactaat cggatttaat gaagatgaga tccggtcatt
      421 tgtccctcgt gaaattcgat gtgttgccag aaatctgatc tatttacaaa acaataacat
      481 cttaaattca tagggaggga gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg
      541 taacaagtct gaaggcgaaa caatggttag aacaccatca attgccttat caatttgttg
      601 acttagaaga acgcgatcta agtacagaag aagcagaaga attatgcagt tttgaagaag
      661 tgcaagcaga acagctattt gcgacttggt ctgaggcttt tcacaacatt gcgattgacc
      721 tttcctgtgt aggaaaaaaa caactgattt ttttatgcac acaacatacc aatctcctca
      781 gacgtccttt gattattatc gatgaagttc tatttattgg atacaatgag cagttgatgg
      841 aacagcttat cttaaataaa tagactttaa ctgttttaat caagtcactg agcatttcat
      901 acagttgtaa cagtcgaaca cttagcatca agcgtgttat gtgttctttt tttgacttta
      961 tttttctact atagagacat gctcaattta tgtggtcaaa attcaaatct aggtggggat
     1021 atagagttaa aaatatattg acatgtgtaa atttacatga tatattcatt tctgttgttt
     1081 taagtagcaa agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt
     1141 agaaatgaca tgataagata actgagttgt taaaaaagaa ccacttgtga agcttgattc
     1201 tatcgaaaaa aacttcaaaa aagttcttga caaataacaa atgataagtt atgataaatg
     1261 agttgctgaa agatttcgaa aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt
     1321 acttgtctct ctgatatgat ataaaagttg ctacggcagc caaaaacgaa tcggggaaca
     1381 ctcc
//
LOCUS       DACECB010000153         1326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.165, whole genome shotgun sequence.
ACCESSION   DACECB010000153 DACECB010000000
VERSION     DACECB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.165"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..1165
                     /locus_tag="IWO04_002830"
     CDS             86..1165
                     /locus_tag="IWO04_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0214358.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          356 c          364 g          247 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccat
//
LOCUS       DACECB010000154         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECB010000154 DACECB010000000
VERSION     DACECB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.166"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1009)
                     /locus_tag="IWO04_002831"
     CDS             complement(<1..1009)
                     /locus_tag="IWO04_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAQ0214359.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IV"
     gene            1134..>1316
                     /locus_tag="IWO04_002832"
     CDS             1134..>1316
                     /locus_tag="IWO04_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214360.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIV"
BASE COUNT          422 a          219 c          211 g          464 t
ORIGIN      
        1 ctacaatatc tgaaagtgtc gatgcggctc gatctagatc ttttatggta cgaagacgtt
       61 ctttagattc cttatttttg gcgcgtctaa aaacagtttc aaaaaaatta gatagtgcta
      121 aaatttgttc atccatagcc ttacgtttgt atatgaaaat aaatgccact aacaacgcca
      181 gttttttctg ttcactcatt cgttgaatta attgagcttt agctttaaat gcataatttg
      241 caagaacttt tattttccct tcaggaatag attccatctt ccatttttca gtagagaaag
      301 cttgaaattg ctttagtcgg tcaaaacctc tggctatttc tttatgacta tcgtcagcta
      361 acggggttct caaaatgtcc attttgatta ttgccccata aactggagtt ccaattaatt
      421 ctaaatgatt gagaagccgc tttctctcat cttcagttgg aaccaaagac aattgtcggt
      481 ataaatgctc ttcagctttt tcaattgcac tagcaacaaa acgagaaaaa gtagtaattc
      541 caggtaaaac aatcttttca tccagacatt tttttagaag catatcaaac aacatatctg
      601 aagattctgt tgtatacagc gctctattta aaagccagtt agctaggtat gtttctattt
      661 ccggttggtt gaaatcttga tatccataat gtgattttat caattttgtg tgctgatacc
      721 tggtctgtct cctgctatag tagataattt ctccaggatc aatatttagt tgagttgcta
      781 cataactcaa tacattttgg ggaatattgg taagatctaa tataaaagtt cctaaaaaac
      841 gtagtgagac taactgtagt gcaaaaccta atctagtagc tggtcgtcgt atagtctcca
      901 tcaattctaa atctttttca tgcaaataaa aaaattggtt taattgttct gtactaggtt
      961 ctcctaaata aatgccaaat gcttttttgt tacttttgat ttcatccatt taaaccctcc
     1021 atgaagtaaa ttttaagtct acataaaatt tatttttaaa aatgctatga tagcagtaaa
     1081 actacaagta aattttacat gtaaattata tcacttttaa aaaggagttt tttatgggaa
     1141 aaattggcta tattcgagta tcttcacaag atcaaaattt ggatcgacaa ttggcaatga
     1201 tggaaggact taacattgat aaattattcc aagaaaaagc cagtggtaaa gatactgagc
     1261 gtcctgagtt tcaaaaatta ctaaattata tccgtgaagg tgattgcgtc atcgtt
//
LOCUS       DACECB010000155         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECB010000155 DACECB010000000
VERSION     DACECB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.167"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IWO04_002833"
     CDS             52..>1302
                     /locus_tag="IWO04_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0214361.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACECB010000156         1298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.168, whole genome shotgun sequence.
ACCESSION   DACECB010000156 DACECB010000000
VERSION     DACECB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.168"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            143..400
                     /locus_tag="IWO04_002834"
     CDS             143..400
                     /locus_tag="IWO04_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214362.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1045)
                     /locus_tag="IWO04_002835"
     CDS             complement(632..1045)
                     /locus_tag="IWO04_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0214363.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          166 c          162 g          480 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaa
//
LOCUS       DACECB010000157         1278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECB010000157 DACECB010000000
VERSION     DACECB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.169"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1278
                     /locus_tag="IWO04_002836"
     CDS             82..>1278
                     /locus_tag="IWO04_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0214364.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNT"
BASE COUNT          429 a          250 c          245 g          354 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaatacc
//
LOCUS       DACECB010000158         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.170, whole genome shotgun sequence.
ACCESSION   DACECB010000158 DACECB010000000
VERSION     DACECB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.170"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWO04_002837"
     CDS             200..742
                     /locus_tag="IWO04_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214365.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWO04_002838"
     CDS             832..>1246
                     /locus_tag="IWO04_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0214366.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACECB010000159         1219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECB010000159 DACECB010000000
VERSION     DACECB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.171"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..1196
                     /locus_tag="IWO04_002839"
     CDS             6..1196
                     /locus_tag="IWO04_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0214367.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          371 a          252 c          274 g          322 t
ORIGIN      
        1 tccctatgaa cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga
       61 atgaattatt ccgtgtccat ttggagaaag cagtcaatac gcttctcaaa acggagttaa
      121 cggctttcct agattacgaa aagtatgatc gcattggttt taacacgggt aattctcgta
      181 acggctccta tgaccgtacg gtcaagaccg agtatgggga acttcatctc cagattccgc
      241 gcgaccgcaa tggtgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgaca
      301 cgttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      361 acttgattga gaaaatgtat gggcattact acacgcccca aaccatgtcc aatataacaa
      421 aatcatttac agaagaggta acggcgttta atgggcggga gcttcatgac cgttatgctg
      481 ctatttatat ggacgcaacg tatattccgt taaagcggaa aaccgtcgcc aaggaagcta
      541 ttcatatcgc agttggcatt cgcccggacg gatcaaagga agtattgagc tatgcgattg
      601 caccgactga atccatcacg atttgggagg aaattttatt ggaccttcaa gagcgcggtt
      661 tgaaaaatgt cctcctgttc atcacggatg gcttaaaggg gatggtagga gcgattagtc
      721 ggttctatcc caaagctcgt tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc
      781 ataaagtgcg tgtcgatgat cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag
      841 cctcatctaa agaggtggcg ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca
      901 gctatccaaa agtggttgaa tcgattcttt cgaacgatca cttgctcact ttctatgatt
      961 tccccttggc catacgcaag agtatttatt ctacgaactt gattgaatcc tttaataaac
     1021 aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt
     1081 tcttagtctc gtcttttgat acttacaacc aaaaattcct aggtcgcagt cataaaggtt
     1141 tccaacaggc cgaaggcgag ctcgaacaaa tgctaagcca actgattgag aattagaaac
     1201 aatctgcagt agggaagaa
//
LOCUS       DACECB010000160         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.172, whole genome shotgun sequence.
ACCESSION   DACECB010000160 DACECB010000000
VERSION     DACECB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.172"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          365 a          200 c          230 g          397 t
ORIGIN      
        1 aggaaaaggc gttcaattaa attatattgc ccattttagt aataccacta cctataatta
       61 tagttctaac caaaattatg ttataaaata attaaaactt taaactagga ggaatgcttt
      121 atgaaattaa caaaaattgc tgtaatttta gctgttattg ctggcattat tttatatttt
      181 gtattgcctg ctaatcaatt cttcctttac tttggaatac ctgcgatttt aatagtaaat
      241 ggcgtaagaa tctttgaaca acgaaaaaaa tgtagctcta cctaatcgtt acaaaacaac
      301 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcca
      361 ctttttgtat tgtagagcgt gaattttgag ggggattcaa gggggagctt gcgccccttt
      421 gacaagcggt tatttgcacc aaatacctcc gtatttggct agcaatcctt ctaaaaaata
      481 cacctatcga tcagagtcaa aacaaatcaa ttttcgagtg agcgaatccg aatttgcaaa
      541 gctgaagctt tgcaaatgag tgtgccggct tccgtaaaaa tacatatggc tataatttgc
      601 gttttaaggc gttttaaacg ttctggtata taattatacc aaaacaactt taaaacgcct
      661 tagatctaat tttagaggtc ttatagaaca ttttagggtt gttagggctt gccctgaccg
      721 cctgtaaggc gctttgagaa caagataagg gttttatact aagactgtct taaaacggct
      781 taaacaaacg ctcagaactt ttcaaaggag ttctttatga actgttttta agcaaaggag
      841 cctttaggcg actgcgcagt ataatgtgag agcgtagcga tcacacattt ttagtgaccg
      901 tagggagcga gtttcccagt ttagaaaaat tcgcgaactt gtttgaggaa aaaagcagga
      961 gttttttagg cgggaaattt tgcctcctct tttataatct ctttttaaac ctctttttaa
     1021 acctctttac cgtcggctgt atgcttactc tcccaaggtg tgcagaatct tttgggggtc
     1081 tttttgtctg tttttagggg tctttaatta ggggcttgac ccctattaga aagctcttta
     1141 taattttgtt aagtagttaa tcttgaagga ggattttatg gcaaatgaaa tt
//
LOCUS       DACECB010000161         1151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECB010000161 DACECB010000000
VERSION     DACECB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.173"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            53..>1151
                     /locus_tag="IWO04_002840"
     CDS             53..>1151
                     /locus_tag="IWO04_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAQ0214368.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          366 a          216 c          225 g          344 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctactcctt
      361 cgaggagaaa atcccccatt tctcaacttt tggaaagaat tatgttcgcc gatttagaga
      421 aacgacggtg tttgaggata tttttgccta tattttagaa caagcagtta aagcaggttt
      481 tgtaacagag gataatttgt atctggattc tacccacatc aaagctaacg ccaataaaca
      541 taagtttacg aaagaaatga cccatgatga agcgaaagcc tatcaggatg aattagaaga
      601 tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct tttactttgg acttagaaaa
      661 ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat ccggaaagcg gctactacgt
      721 caaaggcgaa cgagaaaaac aatttgctta ttctgtccat acttcatgcg acgacaatgg
      781 atttattctt agcacaatca tcactcctag aaatattcat gacagtcaag tcgcctttca
      841 gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat tgtgttgtcg ctgatgctgg
      901 gtataagaca cctaagtttg ttcacttttt gactcatttg aaccttcgac cttgtttgcc
      961 ttattcaagg cccaaaggga aaaaggagct attatcaaaa aatgagtttc tctatgacga
     1021 gtattttgat tgttatattt gtccgcaaga tcaaatgctc gctttttcaa cggttactcg
     1081 agaagggtac cgagaataca aatccaatcc aaaagaatgt gtgaactgtc ctctgctaaa
     1141 ccaatgtacc a
//
LOCUS       DACECB010000162         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECB010000162 DACECB010000000
VERSION     DACECB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.174"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWO04_002841"
     CDS             <1..355
                     /locus_tag="IWO04_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214369.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..985
                     /locus_tag="IWO04_002842"
                     /pseudo
     CDS             615..985
                     /locus_tag="IWO04_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="IWO04_002843"
     CDS             985..>1137
                     /locus_tag="IWO04_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0214370.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DACECB010000163         1121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECB010000163 DACECB010000000
VERSION     DACECB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.175"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IWO04_002844"
     CDS             <1..537
                     /locus_tag="IWO04_002844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214371.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..>1121
                     /locus_tag="IWO04_002845"
     CDS             564..>1121
                     /locus_tag="IWO04_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214372.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKK"
BASE COUNT          476 a          175 c          252 g          218 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa g
//
LOCUS       DACECB010000164         1108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.176, whole genome shotgun sequence.
ACCESSION   DACECB010000164 DACECB010000000
VERSION     DACECB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.176"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1108
                     /locus_tag="IWO04_002846"
     rRNA            <1..>1108
                     /locus_tag="IWO04_002846"
                     /product="23S ribosomal RNA"
BASE COUNT          342 a          219 c          319 g          228 t
ORIGIN      
        1 aggagccgat gaaggacggg actaacaccg atatgctttg gggagctgta cgtaagctat
       61 gatccagaga tttccgaatg ggggaaccca acatctttaa taggatgtta cgattgtgtg
      121 aatacatagc acattcgagg tagacgcaga gaactgaaac atctaagtac ctgcaggaag
      181 agaaagaaaa ttcgattccc tgagtagcgg cgagcgaaac gggaaaagcc caaaccagca
      241 agcttgcttg ttggggttgt aggactccaa tatggtagtt ctttcagata gtcgaatgac
      301 ttggaaaagt cagtcaaaga gggtaaaaac cccgtagacg aaatgtggaa gacacctagg
      361 aggatcctga gtacggcgga acacgagaaa ttccgtcgga atccgggagg accatctccc
      421 aaggctaaat actccctagt gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag
      481 caccccggaa ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa gtcaaagccc
      541 gttaatgggt gatggcgtgc cttttgtaga atgaaccggc gagttacgat tgcatgcgag
      601 gttaagttga agagacggag ccgcagcgaa agcgagtctg aatagggcgt ttgagtatgt
      661 agtcgtagac ccgaaaccat gtgatctacc catgtccagg ttgaaggtgc ggtaaaacgc
      721 actggaggac cgaacccacg tacgttgaaa agtgcgggga tgaggtgtgg gtagcggaga
      781 aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg gctagcctcg
      841 gaattgagaa tgatggaggt agagcactgt ttggactagg ggcccatctc gggttaccga
      901 attcagataa actccgaatg ccattcattc atatccggga gtcagactgt gagtgataag
      961 atccatagtc gaaagggaaa cagcccagac caccagctaa ggtcccaaaa tatatgttaa
     1021 gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt ggcttagaag cagccaccat
     1081 ttaaagagtg cgtaatagct cactagtc
//
LOCUS       DACECB010000165         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECB010000165 DACECB010000000
VERSION     DACECB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.177"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO04_002847"
     CDS             complement(36..992)
                     /locus_tag="IWO04_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214373.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACECB010000166         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.178, whole genome shotgun sequence.
ACCESSION   DACECB010000166 DACECB010000000
VERSION     DACECB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.178"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWO04_002848"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWO04_002848"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACECB010000167         1024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.179, whole genome shotgun sequence.
ACCESSION   DACECB010000167 DACECB010000000
VERSION     DACECB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.179"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWO04_002849"
     CDS             281..973
                     /locus_tag="IWO04_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0214374.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          441 a          145 c          170 g          268 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tata
//
LOCUS       DACECB010000168          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.180, whole genome shotgun sequence.
ACCESSION   DACECB010000168 DACECB010000000
VERSION     DACECB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.180"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            707..>998
                     /locus_tag="IWO04_002850"
     CDS             707..>998
                     /locus_tag="IWO04_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214375.1"
                     /translation="MTDKNNEIVNEIEKNGNRVSNSRLSSYACRNFEISFDRISLVGD
                     LIESEELAFSSFISNSVYFNIFDLHTSQVKGQSVDNVVYFEYDKFKGKAHERR"
BASE COUNT          350 a          131 c          198 g          319 t
ORIGIN      
        1 cgcaagtaaa gtatagtggg ttatacttta catggaagtt gtaccgaaat attgtgttgt
       61 gaagtctata tgattttaaa attatgaagt ctatatgcgt gcgtaaagat atttaaaggg
      121 attaaaaata ttaaaaataa aaacagaata aaataagtca tcagaagctc atatttgcgt
      181 tctaagacgt tttaaatcaa ttcgagtata tttacacgtg attattactt ttcgtcggca
      241 gaagcgttaa aaatggctat aaataataaa gacaagggcg aacaacgtga gcaacgttta
      301 cccttgtctt tattatttta ggcatttagc ttttgctgag cgacgaaaag taattacgtg
      361 ataaaactac tttttagagg catgatcaaa cgcattgcgt ttgtcaggtg tgggcggaag
      421 cccacggttt ttaaggggcg cttttagccc ctttggaatt tatttgattt aaataatatt
      481 tagttttaaa attaggataa cgaaaaaaag acaaacgaaa agcaggcgac tttggcgagt
      541 ccctgcttag tggaataaca aaatgcagtt gttattcatt cagacatagt atatcaagtt
      601 ttgattttga atacaaaaca aattggacga atggatacag gcaaaattta ttttgcaatt
      661 ccactcgtcc ttttttcgtt ccaaaaaatt taaggagcga gataaaatga ctgataaaaa
      721 taacgagatt gtgaacgaga ttgagaaaaa tggtaaccga gtatctaata gccggttatc
      781 gtcctatgcg tgtaggaatt ttgaaatttc atttgaccga atttcactag ttggagattt
      841 gattgaaagt gaagaattag catttagtag ctttatttca aactctgttt attttaatat
      901 ttttgaccta catacaagcc aagtaaaagg gcaatccgtt gataatgtgg tttattttga
      961 atacgacaaa tttaaaggga aagctcatga gagaagaa
//
LOCUS       DACECB010000169          979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.181, whole genome shotgun sequence.
ACCESSION   DACECB010000169 DACECB010000000
VERSION     DACECB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.181"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..602)
                     /locus_tag="IWO04_002851"
     CDS             complement(<1..602)
                     /locus_tag="IWO04_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0214376.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          320 a          173 c          187 g          299 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga gaaatcacac
      901 aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa tctagaaaca
      961 ttttgaataa aaactatta
//
LOCUS       DACECB010000170          879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECB010000170 DACECB010000000
VERSION     DACECB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.182"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..>879
                     /locus_tag="IWO04_002852"
     CDS             76..>879
                     /locus_tag="IWO04_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0214377.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWMPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDVDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT          312 a          161 c          162 g          244 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga tgcctgacgt cattatcggg
      361 cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      421 atatttgatg ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      481 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      541 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      601 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      661 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      721 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      781 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      841 aacgagaatt ctaacgggat tctgcgtcgt aatggactg
//
LOCUS       DACECB010000171          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.184, whole genome shotgun sequence.
ACCESSION   DACECB010000171 DACECB010000000
VERSION     DACECB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.184"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IWO04_002853"
     CDS             <1..861
                     /locus_tag="IWO04_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0214378.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACECB010000172          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.185, whole genome shotgun sequence.
ACCESSION   DACECB010000172 DACECB010000000
VERSION     DACECB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.185"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..518)
                     /locus_tag="IWO04_002854"
     CDS             complement(<1..518)
                     /locus_tag="IWO04_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0214379.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDP"
     gene            complement(529..735)
                     /locus_tag="IWO04_002855"
     CDS             complement(529..735)
                     /locus_tag="IWO04_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0214380.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          233 a          191 c          140 g          292 t
ORIGIN      
        1 ggatctgtat gatcgcccca gatgttttgt gttacccata aatgcgattt gattcccggt
       61 tggttataag gagtgtccaa cgttaatgga ataccatatt tcattgctga atctctagcc
      121 aattcaacgt atgccttgta gtttttctca aacgttgctt tatcatgtgt gtgttgtaac
      181 tcaatctgca caggactgtt ggcattagca tacgaaccag caccgtactg tacataacca
      241 ggttgaccga cttgataaac aattccgccg tctcccacaa tataagcagt ataagcgcta
      301 gtccatgaac gttgcatata ctgcgcttca ttgcgtcctg ttgctgtttc attagccgtt
      361 tcatgcagta aaatatactg attatttgct acttgtgagc taccttcatt tgcgcccaaa
      421 ttaaattcat tgttgatagt ataggcaaat ccgttaattg gcaataaaaa aagagccgtt
      481 aacaggctca tcgcagtaat agtaattttc tttttcatat ctatcctcct atttatctga
      541 tgaaattttc ttatacgttc gatttgatac acctaaaata ctccctagaa atgctccaaa
      601 acctgtaata atcatcaccg ttatatctgt gtaccgccag ttaacacctt ttccgacaag
      661 accgacaaaa gtcgctaaag cggggataac tactatagcg atccatttca aaatatcaaa
      721 ggttctattg ttcatactat ccacctccct tcattggttg ggctactttc tttttcaatc
      781 gcttgttttc ttctatcaat atttctcttt ctgaaagcaa ggcatcaatt tgcccgtcca
      841 gttgttcaat tttatc
//
LOCUS       DACECB010000173          825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECB010000173 DACECB010000000
VERSION     DACECB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.186"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(304..377)
                     /locus_tag="IWO04_002856"
     tRNA            complement(304..377)
                     /locus_tag="IWO04_002856"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Ile,seq:cat)
     gene            complement(566..>825)
                     /locus_tag="IWO04_002857"
     CDS             complement(566..>825)
                     /locus_tag="IWO04_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0214381.1"
                     /translation="YIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFNM
                     RNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          270 a          153 c          139 g          263 t
ORIGIN      
        1 ttttaatagc ttcaaatata aaagagttta aagcgtaaca cacttatggg gaagtggctt
       61 ggggtgcgct ttaaactctt ctttattatc atctcacaat ttaacccaaa tgtctttcta
      121 tttaaaaatc aaagtaaaac ttttcaaata tacagaaata taactatgtg aaacatcctt
      181 tcattaatcc ataaaaggat acataaaaaa gccactcatt tgagtggcaa tgaagaaaag
      241 ctttagcttg tataatactc ttcaaaaaat tctaacacag aacgattcaa atggctacgt
      301 taatgtaccc tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctgacc
      361 aactgagcta agggtactgg ttgttgccac ataaagccat aaacaatcaa ccagtagaat
      421 gtgtggcaac aaacctgtta tcgcatagct tggagtgtga ctattatggg tgatagtgaa
      481 gatatgcgat aacatcacta ttttatcgaa tgatttttat agttgtcaat atagttatgt
      541 aatgctcctc aacgaggagc tatttttatc gtttaggaat atttaaatac caacgtttgt
      601 catggaaatc ttgcgcaccg cctttagtgt ttccctctgg atcattcgtt gcccgcatca
      661 tgacatagac tttcttatta gggaagttac gcatgttaaa agatacatga tagccaacat
      721 ttccagaagt attataagct tgatttacat ctgatctata aattccatca gctcttactc
      781 gagctaattc tttcccagta ttgtaatcca taatgaagat atact
//
LOCUS       DACECB010000174          815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECB010000174 DACECB010000000
VERSION     DACECB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.187"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(72..569)
                     /gene="dfrG"
                     /locus_tag="IWO04_002858"
     CDS             complement(72..569)
                     /gene="dfrG"
                     /locus_tag="IWO04_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0214382.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          253 a          161 c           86 g          315 t
ORIGIN      
        1 caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat
       61 taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac
      121 ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc
      181 acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc
      241 tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct
      301 tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag
      361 gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt
      421 gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat
      481 tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta
      541 tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac taatttacca
      601 tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa
      661 gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt ttcattcaat
      721 tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg taagttaaaa
      781 tgaaaaaagt agtccatttt gaagtacatt ttgtc
//
LOCUS       DACECB010000175          814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.188, whole genome shotgun sequence.
ACCESSION   DACECB010000175 DACECB010000000
VERSION     DACECB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.188"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            27..>814
                     /locus_tag="IWO04_002859"
     CDS             27..>814
                     /locus_tag="IWO04_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0214383.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGL"
BASE COUNT          283 a          144 c          155 g          232 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      661 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatgtcaat gatattgcca tttatttcgc tgatccagga acgccgtctc
      781 aaagaggcct aaacgagaat tctaacggat tgtt
//
LOCUS       DACECB010000176          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.189, whole genome shotgun sequence.
ACCESSION   DACECB010000176 DACECB010000000
VERSION     DACECB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.189"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..780
                     /locus_tag="IWO04_002860"
     CDS             <1..780
                     /locus_tag="IWO04_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0214384.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          244 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccctt t
//
LOCUS       DACECB010000177          781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECB010000177 DACECB010000000
VERSION     DACECB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.190"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWO04_002861"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWO04_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0214385.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          289 a          119 c          146 g          227 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 c
//
LOCUS       DACECB010000178          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.191, whole genome shotgun sequence.
ACCESSION   DACECB010000178 DACECB010000000
VERSION     DACECB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.191"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..>734)
                     /locus_tag="IWO04_002862"
     CDS             complement(109..>734)
                     /locus_tag="IWO04_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ0214386.1"
                     /translation="STYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYV
                     RVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
                     FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLEL
                     FDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          207 a          166 c          139 g          222 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatg
//
LOCUS       DACECB010000179          713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.192, whole genome shotgun sequence.
ACCESSION   DACECB010000179 DACECB010000000
VERSION     DACECB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.192"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..448)
                     /locus_tag="IWO04_002863"
     CDS             complement(<1..448)
                     /locus_tag="IWO04_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0214387.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          123 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aac
//
LOCUS       DACECB010000180          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECB010000180 DACECB010000000
VERSION     DACECB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.193"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..629)
                     /locus_tag="IWO04_002864"
     CDS             complement(54..629)
                     /locus_tag="IWO04_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0214388.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACECB010000181          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.194, whole genome shotgun sequence.
ACCESSION   DACECB010000181 DACECB010000000
VERSION     DACECB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.194"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACECB010000182          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECB010000182 DACECB010000000
VERSION     DACECB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.195"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWO04_002865"
     CDS             154..417
                     /locus_tag="IWO04_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0214389.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="IWO04_002866"
     CDS             417..>670
                     /locus_tag="IWO04_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0214390.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DACECB010000183          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECB010000183 DACECB010000000
VERSION     DACECB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.196"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..>651
                     /locus_tag="IWO04_002867"
     CDS             8..>651
                     /locus_tag="IWO04_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HAQ0214391.1"
                     /translation="MCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIR
                     FQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRL
                     AYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT          235 a          104 c          135 g          177 t
ORIGIN      
        1 tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg
       61 aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga
      121 acaagaggaa agcataagat ttcagaattg ctccttgtac cctgtttatc atggaagcgc
      181 aaaaagcaac atagggattg agcagcttat agaagtgata acgaataaat tttattcatc
      241 aacatacaga aagaagtctg aactttgcgg aaatgtcttc aaaattgaat attcggaaga
      301 aagacaacgt cttgcatatg tacgccttta tggcggaatc ctgcatttgc gggattcggt
      361 tagaatatcg gaaaaggaaa aaataaaaat tacagaaatg tatacttcaa taaatggtga
      421 attatgtaaa attgataagg cttattccgg ggaaattgtt attttgcaaa atgagttttt
      481 gaagctaaat agtgttcttg gagatacaaa gctattgcca cagagagaga gaattgaaaa
      541 tccgctccct ctgctgcaaa caactgttga accgagcaaa cctcaacaaa gggaaatgtt
      601 acttgatgca cttttagaaa tctccgacag tgacccgctt ctacaatatt a
//
LOCUS       DACECB010000184          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECB010000184 DACECB010000000
VERSION     DACECB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.197"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..625
                     /locus_tag="IWO04_002868"
                     /pseudo
     CDS             1..625
                     /locus_tag="IWO04_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          215 a          110 c          117 g          183 t
ORIGIN      
        1 atgactaaag tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag
       61 tatgtcgcta ttttcatgga tgctacttat attcctttaa aacggcaaac cgtctccaaa
      121 gaagcaatct atattgccat tggtatacga gaagacggca ctaaagaagt actaagttat
      181 gcgattgctc cgactgaatc gacctacgtt tggaatgagt tgttacagga catttactcc
      241 agaggggttc atgatgtctt actgtttatt accgatggct taaaaggcat gaaagatacg
      301 attcaccaaa tttatcctaa agctaaatac caacattgct gtgttcacgt ttcccgtaat
      361 attgctcata aagtacgtgt caaagatcga aaagaaatct gtgatgattt taaggctgtt
      421 tatcaagcta gctcaaagga agaggcaaat acctttttag ggagtatgat tgagaagtag
      481 caaaaaactt atcctaaagt gacgcagtca ctgataaaaa atcaagattt attgactttt
      541 tatgagtttc cgcctggtat ccgccgaagc atttactcaa ctaatttaat cgaatctttc
      601 aagaaacaaa tcaagaaata cagcc
//
LOCUS       DACECB010000185          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECB010000185 DACECB010000000
VERSION     DACECB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.198"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>618
                     /locus_tag="IWO04_002869"
     CDS             <1..>618
                     /locus_tag="IWO04_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214392.1"
                     /translation="QDIEERKNAMHGQAKSAEELINQKRHEAKYTPEAMNREEIMLRK
                     RVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQ
                     GSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSSSTSQPKTVDVRENVQNSTSS
                     QGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVDVREN"
BASE COUNT          250 a           91 c          143 g          134 t
ORIGIN      
        1 caagatattg aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc tgaagaatta
       61 attaatcaga agcgtcatga agcgaaatac acgcctgaag caatgaatcg cgaggaaata
      121 atgttaagga aacgtgtaga agggcggaca ggcgctaata ttgacggaaa agaagcgttg
      181 accaaagaaa gaattcaaga agctaaaaat tcaaatacag gattagaaaa attagtcaaa
      241 gaaaatccac aaaattctac aagtagtcat ggtggccgat cagtagatgt ccgagaaaat
      301 attcaaggtt cactgaatgg tcatgttgga cggaatgttg atgtaagaga gaatttacaa
      361 agttcttcaa atgctcaagg tactaagaca attgatgttc gagaaaatct gcaaagtagc
      421 agtacaagtc aaccaaaaac agtagatgtt cgagaaaatg tacaaaattc tacaagtagt
      481 caagggttta aatcagtgga tgtccgagag aatgtgcaag gtagcagtgc aagtcaacca
      541 aaaacagtag atgtccgaga aaatgtacaa agttctacaa gtagtcaagg attcaaaaca
      601 gtagatgttc gggagaac
//
LOCUS       DACECB010000186          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECB010000186 DACECB010000000
VERSION     DACECB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.199"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..>610
                     /locus_tag="IWO04_002870"
     CDS             25..>610
                     /locus_tag="IWO04_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0214393.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLS"
BASE COUNT          223 a          113 c          103 g          171 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa
      541 accgtatcca aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa
      601 gtactgagtt
//
LOCUS       DACECB010000187          606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECB010000187 DACECB010000000
VERSION     DACECB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.200"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..322
                     /locus_tag="IWO04_002871"
     CDS             <1..322
                     /locus_tag="IWO04_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214394.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          217 a          106 c           75 g          208 t
ORIGIN      
        1 agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt atccaagagt
       61 cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc acgccatcag
      121 acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa aacgatatac
      181 tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca tttctcaatt
      241 taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag aaattcagga
      301 tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt tacatataat
      361 tcttgacgct accataatct aaataaccat catattcatc tactacttat ataattgagt
      421 tttattaaac tttttaagtt tctattctat caaatattta aacaatctct gcatccgaaa
      481 agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa tcctgatttc
      541 ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt ccatggataa
      601 taataa
//
LOCUS       DACECB010000188          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.201, whole genome shotgun sequence.
ACCESSION   DACECB010000188 DACECB010000000
VERSION     DACECB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.201"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..523
                     /locus_tag="IWO04_002872"
     CDS             <1..523
                     /locus_tag="IWO04_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214395.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
BASE COUNT          231 a           88 c          134 g          138 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg a
//
LOCUS       DACECB010000189          590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECB010000189 DACECB010000000
VERSION     DACECB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.202"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..523
                     /locus_tag="IWO04_002873"
     CDS             <1..523
                     /locus_tag="IWO04_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0214396.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
BASE COUNT          227 a           90 c          139 g          134 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg
//
LOCUS       DACECB010000190          588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECB010000190 DACECB010000000
VERSION     DACECB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.203"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..150)
                     /locus_tag="IWO04_002874"
     tRNA            complement(65..150)
                     /locus_tag="IWO04_002874"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IWO04_002875"
     tRNA            complement(167..238)
                     /locus_tag="IWO04_002875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IWO04_002876"
     tRNA            complement(250..322)
                     /locus_tag="IWO04_002876"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IWO04_002877"
     tRNA            complement(340..421)
                     /locus_tag="IWO04_002877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IWO04_002878"
     tRNA            complement(429..501)
                     /locus_tag="IWO04_002878"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IWO04_002879"
     tRNA            complement(505..577)
                     /locus_tag="IWO04_002879"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACECB010000191          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECB010000191 DACECB010000000
VERSION     DACECB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.204"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          145 a          217 c          144 g           72 t
ORIGIN      
        1 cggctggaac cacatctcag acgtgccaga aacgtgttca tgagatgctg gaaccacacc
       61 ccgaacagac ggataccacc tgccagaacc gcctgaacca cctgctgagc acgccggaac
      121 cacctgctcg actcaccggt accacctccc tgacacgcca aaatcacctg ctgggtatgc
      181 cagaaccact tggcggacac tccggaatca tctgccggac atgccggaac cagctctcag
      241 acacaccaga accacgtgcc ggagacgccg gaagcacctg ccgagcatgc cggagccacc
      301 tgctcgactc gccagaaaca cctgccggac acgccaaaat cacctgccgg gtacgccaga
      361 accacctggc ggacactccg aaatcacctg tcgcgcatgc cggaaccacc tctcagacgt
      421 gccggaagca cgtgccggac acgccggaat cacctaccgg acagaccgga accacctgcc
      481 gagcacgccg gagccacctg ttcgactcgc tggaaccacc tgccggacag gccaatatca
      541 actgctggct atgccagaac cacctggggg acagtccg
//
LOCUS       DACECB010000192          568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECB010000192 DACECB010000000
VERSION     DACECB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.205"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>568
                     /locus_tag="IWO04_002880"
     CDS             <1..>568
                     /locus_tag="IWO04_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0214397.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DACECB010000193          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECB010000193 DACECB010000000
VERSION     DACECB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.206"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWO04_002881"
     CDS             <1..354
                     /locus_tag="IWO04_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0214398.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DACECB010000194          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.207, whole genome shotgun sequence.
ACCESSION   DACECB010000194 DACECB010000000
VERSION     DACECB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.207"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            57..>517
                     /locus_tag="IWO04_002882"
     CDS             57..>517
                     /locus_tag="IWO04_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0214399.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACECB010000195          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.208, whole genome shotgun sequence.
ACCESSION   DACECB010000195 DACECB010000000
VERSION     DACECB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.208"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DACECB010000196          478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECB010000196 DACECB010000000
VERSION     DACECB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.209"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..461
                     /locus_tag="IWO04_002883"
     CDS             <1..461
                     /locus_tag="IWO04_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0214400.1"
                     /translation="DLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTEILAVELA
                     QSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLGQRLLDEANRYAKT
                     KQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILEKEI"
BASE COUNT          173 a           70 c           89 g          146 t
ORIGIN      
        1 atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg tttatcgatg
       61 gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact gaaatattgg
      121 cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat caaatactca
      181 ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg ctctatctgc
      241 tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg aatcgttatg
      301 ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat catgcaggta
      361 ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttt cctttaggaa
      421 aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc ctcacttt
//
LOCUS       DACECB010000197          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECB010000197 DACECB010000000
VERSION     DACECB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.210"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>473
                     /locus_tag="IWO04_002884"
     CDS             <1..>473
                     /locus_tag="IWO04_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0214401.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DACECB010000198          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECB010000198 DACECB010000000
VERSION     DACECB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.211"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IWO04_002885"
     CDS             <1..>468
                     /locus_tag="IWO04_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0214402.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACECB010000199          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECB010000199 DACECB010000000
VERSION     DACECB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.212"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IWO04_002886"
     CDS             complement(<1..430)
                     /locus_tag="IWO04_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0214403.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          180 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tct
//
LOCUS       DACECB010000200          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECB010000200 DACECB010000000
VERSION     DACECB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.213"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..432
                     /locus_tag="IWO04_002887"
                     /pseudo
     CDS             1..432
                     /locus_tag="IWO04_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          167 a           86 c           82 g           97 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca aacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aa
//
LOCUS       DACECB010000201          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECB010000201 DACECB010000000
VERSION     DACECB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.214"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..403
                     /locus_tag="IWO04_002888"
     CDS             74..403
                     /locus_tag="IWO04_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0214404.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DACECB010000202          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECB010000202 DACECB010000000
VERSION     DACECB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.215"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWO04_002889"
     CDS             complement(<1..339)
                     /locus_tag="IWO04_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214405.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACECB010000203          413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECB010000203 DACECB010000000
VERSION     DACECB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.216"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(126..>413)
                     /locus_tag="IWO04_002890"
     CDS             complement(126..>413)
                     /locus_tag="IWO04_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAQ0214406.1"
                     /translation="QEYTIKLHTDVNPQTLRSAKSKTISIKKTDQKELFLPAKGFENC
                     SHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIENQQKRQQNFEMEF"
BASE COUNT          114 a           75 c           78 g          146 t
ORIGIN      
        1 agcctagcgt gagcggggcg gattgactgg caagtagatg ggtagctggc gtatgtccta
       61 aaaactgctt ctttccaagc aactcttcat aggcaaaaaa ctggaaagtc cactaacaag
      121 cccattcaga attccatttc aaaattttgt tgcctttttt gctgattttc aatcatttgt
      181 tgtgtgaatt cataatcctt tttagcacga tacattttta catcgtcatt cattatgcga
      241 ctgatttgat agtgaatctg tttagcagaa ggtgtatgag aacaattttc aaaacccttt
      301 gccggtagaa ataactcttt ctgatcagtc tttttaatag aaattgtttt acttttcgca
      361 ctacgtaaag tttgaggatt cacatccgta tgaagtttaa tcgtgtattc ttg
//
LOCUS       DACECB010000204          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.217, whole genome shotgun sequence.
ACCESSION   DACECB010000204 DACECB010000000
VERSION     DACECB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.217"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(204..>409)
                     /locus_tag="IWO04_002891"
     CDS             complement(204..>409)
                     /locus_tag="IWO04_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214407.1"
                     /translation="YTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLI
                     GAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          150 a           48 c           69 g          142 t
ORIGIN      
        1 gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga
       61 cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat
      121 agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaat tccagactcc tgtgtaaaat
      181 gctatacaat gtttttacca tttctactta tcaaaattga tgtattttct tgaagaataa
      241 atccattcat catgtaggtc cataagaacg gctccaatta agcgattggc tgatgtttga
      301 ttggggaaga tgcgaataat cttttctctt ctgcgtactt cttgattcag tcgttcaatt
      361 agattggtac tctttagtcg attgtgggaa tttccttgta cggtatatt
//
LOCUS       DACECB010000205          398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECB010000205 DACECB010000000
VERSION     DACECB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.218"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..371
                     /locus_tag="IWO04_002892"
     CDS             <1..371
                     /locus_tag="IWO04_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214408.1"
                     /translation="FMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQN
                     EREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEI
                     AKENGISRPTIYKIKKANDL"
BASE COUNT          142 a           67 c           77 g          112 t
ORIGIN      
        1 cttttatgaa acagaaaaaa gtagcgttaa aaatattaga tgcacagttt ttagatttta
       61 atactggaaa tgaactcctg gataccgcta tgttcgatat gtttttatca ctgctaagtt
      121 atattgcaca aaacgagcgt gagaaaattc gtgaacgtca gcggcaagga gtattattgg
      181 ctcaaaaagc cggccgatat aagggacgac caatcgagta ttctattaac tcaacagatc
      241 cgcaaaaacg cttagttttt aaaaccgtgg tggacatgtt aaagcaaggc attccagttg
      301 cagagattgc aaaagaaaac gggatctctc gaccaactat ttataaaata aagaaagcga
      361 atgatttgta gaaatatact acattcattc gcttttct
//
LOCUS       DACECB010000206          397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECB010000206 DACECB010000000
VERSION     DACECB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.219"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>397)
                     /locus_tag="IWO04_002893"
     CDS             complement(<1..>397)
                     /locus_tag="IWO04_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0214409.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIV"
BASE COUNT          111 a           83 c           62 g          141 t
ORIGIN      
        1 ttacaatttc ggcagaaagt ttctctttaa tatcctgata aaccgttgat aaatcaattc
       61 cattttggtc aatcttattg ataaaaaaga ttgtgggaat ccccattttc ctaagtgcat
      121 gaaataatat acgagtttgt gcttgtacgc catcttttgc agaaatcagt agaattgccc
      181 catctaaaac tgataatgaa cgatatactt ctgctaagaa atccatatgt cctggcgtgt
      241 ctatgatgtt caccttcgta ttttcccact gaaaagaggt tattcctgtc tgaattgtaa
      301 ttcctctctg acgttctaaa agcgtattat ccgtcctcgt tgtacctttg tccacgcttc
      361 ctaattctgt aatcgctcca ctgttatata ataagct
//
LOCUS       DACECB010000207          384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECB010000207 DACECB010000000
VERSION     DACECB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.220"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>384
                     /locus_tag="IWO04_002894"
     CDS             <1..>384
                     /locus_tag="IWO04_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0214410.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVC"
BASE COUNT          141 a           69 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tgca
//
LOCUS       DACECB010000208          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECB010000208 DACECB010000000
VERSION     DACECB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.221"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>379
                     /locus_tag="IWO04_002895"
     CDS             <1..>379
                     /locus_tag="IWO04_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1W family transposase"
                     /protein_id="HAQ0214411.1"
                     /translation="QIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKY
                     LNNLIEQDHRPVKRRNKFYRSLRT"
BASE COUNT          144 a           64 c           71 g          100 t
ORIGIN      
        1 atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg gatgaaacgt
       61 acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca gatggtttaa
      121 ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct tttcttaagc
      181 ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc ccctctatta
      241 caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa catcgtacca
      301 ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga cgcaataaat
      361 tctatcgaag tttacgcac
//
LOCUS       DACECB010000209          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECB010000209 DACECB010000000
VERSION     DACECB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.224"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            80..>366
                     /locus_tag="IWO04_002896"
     CDS             80..>366
                     /locus_tag="IWO04_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0214412.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          129 a           59 c           76 g          102 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcg
//
LOCUS       DACECB010000210          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECB010000210 DACECB010000000
VERSION     DACECB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.225"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..269
                     /locus_tag="IWO04_002897"
                     /pseudo
     CDS             1..269
                     /locus_tag="IWO04_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          123 a           46 c           64 g          108 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc c
//
LOCUS       DACECB010000211          340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECB010000211 DACECB010000000
VERSION     DACECB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.226"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>340
                     /locus_tag="IWO04_002898"
     CDS             <1..>340
                     /locus_tag="IWO04_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214413.1"
                     /translation="AYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAY
                     SGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLE
                     ISDSDPLLRY"
BASE COUNT          126 a           48 c           64 g          102 t
ORIGIN      
        1 ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag
       61 aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga
      121 ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata
      181 gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc
      241 tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc
      301 ttttggaaat ctcagatagt gatccgcttc tacgatatta
//
LOCUS       DACECB010000212          332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECB010000212 DACECB010000000
VERSION     DACECB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.227"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>332
                     /locus_tag="IWO04_002899"
     CDS             <1..>332
                     /locus_tag="IWO04_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0214414.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DACECB010000213          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECB010000213 DACECB010000000
VERSION     DACECB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.228"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWO04_002900"
     CDS             complement(<1..>321)
                     /locus_tag="IWO04_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0214415.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACECB010000214          317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECB010000214 DACECB010000000
VERSION     DACECB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.229"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>317
                     /locus_tag="IWO04_002901"
     CDS             <1..>317
                     /locus_tag="IWO04_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0214416.1"
                     /translation="IYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLG
                     WKKLFGKENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTA
                     GKTV"
BASE COUNT          123 a           57 c           78 g           59 t
ORIGIN      
        1 atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaaata tacctatgaa
       61 gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg aaaagtaccg
      121 atagatctag gttggaagaa gttatttggc aaagagaacg acggaaaaga gaaggaagaa
      181 gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct acaagtcctt
      241 gaaaaagaaa ccaagccacc acagccttat accgaaggta cgttgatcac ggcgatgaaa
      301 acagctggga aaaccgt
//
LOCUS       DACECB010000215          309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECB010000215 DACECB010000000
VERSION     DACECB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.230"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWO04_002902"
     CDS             complement(<1..302)
                     /locus_tag="IWO04_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0214417.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DACECB010000216          296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECB010000216 DACECB010000000
VERSION     DACECB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.231"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>296)
                     /locus_tag="IWO04_002903"
     CDS             complement(<1..>296)
                     /locus_tag="IWO04_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HAQ0214418.1"
                     /translation="VLKGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQF"
BASE COUNT          105 a           59 c           46 g           86 t
ORIGIN      
        1 aaaaactgcg cttcatattg tttaaattca gcaacggata gaccaaatac ccgaaaacca
       61 tagtcaaaat gagcaaagac atctgcttct ataacaacta gtgcttctga aagttgtgca
      121 aaatactgag gaatcacaat agcaggatcc atatctttga cttcgtcatc taaataatca
      181 aattgaccat tatgatgcac actgagtaat tttaaatcgt aatctttatc ggctaaatag
      241 tcgatgatcc gatccttttc agaagcgatg taaccgattt cgatgccttt taaaac
//
LOCUS       DACECB010000217          266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECB010000217 DACECB010000000
VERSION     DACECB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.232"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..>266
                     /locus_tag="IWO04_002904"
     CDS             85..>266
                     /locus_tag="IWO04_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0214419.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           84 t
ORIGIN      
        1 tacctatgaa atattttcac gtgttaaagt attcctcgtt gaacacaaca aataaaactt
       61 tttaacgaat aaaggagatt tttcatgaca tcacttaaaa ctttactttt tggaacaact
      121 ttggctgccg gcgctgcatt cttcatggga acgactgctc atgcggacga agcttatacc
      181 gttcaatcag gtgatactct atcaacgatt tctcaaaaat acgttggtga taactccttg
      241 atcaatgcca ttgcagaatc aaattc
//
LOCUS       DACECB010000218          262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.233, whole genome shotgun sequence.
ACCESSION   DACECB010000218 DACECB010000000
VERSION     DACECB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.233"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          110 a           33 c           47 g           72 t
ORIGIN      
        1 caagacgaag attcacttat gaagacaatg agttattcag agatacactt aatgatccta
       61 atccactttt cgataattta ttaagaaata aaaggggggg attgtcatgc aattaattct
      121 aaagattagt acgcatgatt tcttagaact tgacgaatta tgtgaatcag taaagaacgc
      181 aacaaatgaa aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa
      241 gatgaaaaat gaaatagaga gc
//
LOCUS       DACECB010000219          261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECB010000219 DACECB010000000
VERSION     DACECB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.234"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          110 a           35 c           44 g           72 t
ORIGIN      
        1 caagacgaag gtttacctat gaagacaacg agttattcag agatacactt aatgatccta
       61 atccactttt cgatagttta cttaaaaaat aaagaggtga ttgtcatgca attaattcta
      121 cagattagta cacatgattt cttagaactt gacgaattat gtgaatcagt aaagaaagca
      181 acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga agaattaaag
      241 atgaaaaatg aaatagagag c
//
LOCUS       DACECB010000220          245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.235, whole genome shotgun sequence.
ACCESSION   DACECB010000220 DACECB010000000
VERSION     DACECB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.235"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>245)
                     /locus_tag="IWO04_002905"
     CDS             complement(<1..>245)
                     /locus_tag="IWO04_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0214420.1"
                     /translation="AYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQ
                     QCCVHVSRNIAHKVRVRGRKEICEDFKLVYQANSKEE"
BASE COUNT           75 a           57 c           30 g           83 t
ORIGIN      
        1 cctcttcttt tgaattcgct tggtaaacca atttgaaatc ctcacaaatt tcttttcgac
       61 ctcgaacacg aactttatga gcgatatttc tagatacatg cacacaacat tgttgaaatt
      121 gggcattcgg atacacacta tggatacttt cttcaataca gcttaatccg tctgtcacta
      181 ctaataaaac ttcttctaac ccacgttttc taagatcttg aagaacttct ttccaaatat
      241 aagca
//
LOCUS       DACECB010000221          241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.denovo.236, whole genome shotgun sequence.
ACCESSION   DACECB010000221 DACECB010000000
VERSION     DACECB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.denovo.236"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..220)
                     /locus_tag="IWO04_002906"
     CDS             complement(<1..220)
                     /locus_tag="IWO04_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0214421.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DACECB010000222        11712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.083, whole genome shotgun sequence.
ACCESSION   DACECB010000222 DACECB010000000
VERSION     DACECB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.083"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..1958)
                     /locus_tag="IWO04_002907"
                     /pseudo
     CDS             complement(18..1958)
                     /locus_tag="IWO04_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2104..2679
                     /locus_tag="IWO04_002908"
     CDS             2104..2679
                     /locus_tag="IWO04_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0214422.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            complement(3030..4343)
                     /locus_tag="IWO04_002909"
     CDS             complement(3030..4343)
                     /locus_tag="IWO04_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAQ0214423.1"
                     /translation="MATLHENRLLFNSNVTVSHSGGNLSSDSGLILAKEFMNKFEFSQ
                     ILCKNIQIQDDRLYHVHENESILEQIILQLIAGYPTDSSANILATDPIFQAVLSKKRL
                     ASQSSISRFWDRISTENIVQLQKVNQILIDKARTIRNTNEMIFDLDSTHSDTFGNQEM
                     TDYNAHYQTTGYHPLVAFDGLTGDFLKAELRSGNVYTSKGVAAFTRPLFEHYHSVTPV
                     STIMVRADSGFAMPDLYELCEEYDSLYTIRLKSNRNLYRIADQFVTIKDHHDWSKKEV
                     HYYNATYQAKSWGKSRRICIKSTREASELLFRHEFIITNFSKDVSPEMVFQTYSKRGT
                     MENYIKEAKNGFYLDKTDSPRFIENHARMIVSLLAYNIVNFMRTLCFTNETKGYQVST
                     IRLFLFKIAGKIVHSGRRQYLKLSSYHVYQKLFYRILQNIQVLQC"
     gene            4573..5268
                     /gene="vanR"
                     /locus_tag="IWO04_002910"
     CDS             4573..5268
                     /gene="vanR"
                     /locus_tag="IWO04_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0214424.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5246..6400
                     /locus_tag="IWO04_002911"
     CDS             5246..6400
                     /locus_tag="IWO04_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0214425.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            6615..7583
                     /locus_tag="IWO04_002912"
     CDS             6615..7583
                     /locus_tag="IWO04_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0214426.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7576..8607
                     /gene="vanA"
                     /locus_tag="IWO04_002913"
     CDS             7576..8607
                     /gene="vanA"
                     /locus_tag="IWO04_002913"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0214427.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            8613..9221
                     /locus_tag="IWO04_002914"
     CDS             8613..9221
                     /locus_tag="IWO04_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0214428.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            9627..10565
                     /gene="vanY"
                     /locus_tag="IWO04_002915"
     CDS             9627..10565
                     /gene="vanY"
                     /locus_tag="IWO04_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0214429.1"
                     /translation="MVKEETENEEVVFLLLLLLLFLIYLGYDYVNEALFSQEKVEFQN
                     YDQNPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHD
                     ELINGYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMG
                     AEYALPAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTG
                     IQYEPWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKN
                     TTIHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            10718..11203
                     /gene="vanZ"
                     /locus_tag="IWO04_002916"
     CDS             10718..11203
                     /gene="vanZ"
                     /locus_tag="IWO04_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0214430.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         3694 a         2090 c         2394 g         3534 t
ORIGIN      
        1 attttattct ttatttaaaa ctttgcaaca gaacccttat atgtaagtcc gggtgtggct
       61 tcggccatct tgctcaagcc aatattcatt cccattccta aaagggcagc catgataatg
      121 attgtttctt ccttatctgg ttttcgatta ttggaagcat gagtgaattg ctcatgaaat
      181 cctgttatat gggccacatc catgagtaaa tcagttaatt ttattcttgg tagcatctga
      241 taaaggcttg cactaaattt ttttgcttct tctggaacat ctttttctaa gcgtgcaagt
      301 gatagctttc ctttttcaag agaaacccca tctaacttat tggaattggc agctaaccac
      361 tttaaccttt cattaaagct gctggttctc tccgttatat aatcttcgaa tgataaacta
      421 actgataatc tcgtattccc cttcgattga ttccatgtat cttccgaaaa caaatattcc
      481 tcaaaatccc tatattgtct gctgccaaca atggaaacat ctcctgcccg aacatgctcc
      541 cgaagttctg ttaaaacagc catttcatag taatgacgat taattgttgt accatcatcc
      601 tcgtataaat gtcttttcca tcgttttgaa ataaaatcca caggtgagtc atcaggcact
      661 tttcgctttc cagattcgtt cattcctcgg ataatctcaa cagcttgtaa aagtggctca
      721 tttgcctttg tagaatgaaa ttccaatact cttaatagcg ttggcgtata ttttcttagt
      781 gaataaaacc gtttttgcag taagtctaaa taatcatagt cggcaggacg tgcaagttcc
      841 tgagcctctt ctactgaaga gacaaaggta ttccattcaa taaccgattc taaaacctta
      901 aaaacgtcta atttttcctc tcttgcttta attaatgctt gtccgatgtt cgtaaagtgt
      961 ataactttct catttagctt tttaccgttt tgtttctgga tttcctcttg agccttacga
     1021 ccttttgata acaaactaag tatttgccta tcatgaattt caaacgcttt atccgttagc
     1081 tcctgagtaa gttgtaataa atagatggtt aatatcgaat aacgtttatt ttcttgaaag
     1141 tcacggaatg catacggctc gtatcttgag cctaagcgag acagctgcaa caggcggtta
     1201 cggtgcaaat gactaatttg cactgtttct aaatccattc ctcgtatgta ttcgagtcgt
     1261 tctattattt ttagaaaagt ttcgggtgaa ggatgacccg gtggctcttt taaccaaccc
     1321 aatatcgttt tattggattc ggatggatgc tgcgaggtaa taatcccttc aagcttttct
     1381 ttttgctcat ttgttagaga tttactaacc gtattaaata gcttcttttc agccattgcc
     1441 cttgcttccc acaccattct ttcaagtgta gtgatagcag gcagtataat tttgtttttt
     1501 cttagaaaat ctatgcattc atgcagtaga tgaatggcat caccattttc caaagctaat
     1561 tgatgaaggt acttaaatgt cattcgatat tcactcaggg taaaagttac aaagtcgtat
     1621 tcacttcgaa tttctttcaa atgatcccaa agtgtatttt ccctttgagg ataatgatca
     1681 agcgaggatg gactaacacc aatctgtttc gatatatatt gtatgaccga atctgggatg
     1741 cttttgatat gagtgtatgg ccaaccggga taccgaagaa cagctaattg aacagcaaat
     1801 cctaaacggt tttcttccct ccttcgctta ttaactattt ctaaatcccg tttggaaaaa
     1861 gtgaagtagg tccccagtat ccattcatct tcagggattt gcataaaagc ctgtctctgt
     1921 tccggtgtaa gcaattctct acctctcgca attttcattc agtatcattc catttctgta
     1981 ttttcaattt attagttcaa ttatatatca atagagtgta ctctattgat acaaatgtag
     2041 tagactgata aaatcatagt taagagcgtc tcataagact tgtctcaaaa atgaggtgat
     2101 attttgcgga aaatcggtta tattcgtgtc agttcgacta accagaatcc ttcaagacaa
     2161 tttcagcagt tgaacgagat cggaatggat attatatatg aagagaaagt ttcaggagca
     2221 acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt tacaggaaga tgacatcatt
     2281 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
     2341 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
     2401 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
     2461 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
     2521 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
     2581 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
     2641 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
     2701 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
     2761 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
     2821 tacctgaata attcatactt tttataaact accaaatttt ataattatct gtttagtttt
     2881 atgctcaatg gttctttttc tcaaaaatat ttttctaaag ggtttaattt atttttgcta
     2941 actgattaag tttaagaaag acctcatttt gggcgcactc atcctatcgg atggttaacg
     3001 aatttttttg aatggcgcaa taaacgcaac taacactgca gtacctgaat gttttgtaga
     3061 atccgataaa atagcttctg gtaaacatgg taactactta atttcaagta ttgtcttctt
     3121 ccggaatgaa ctatctttcc agcaatttta aagagaaata agcgaatagt agaaacctga
     3181 taccctttcg tttcattggt aaaacacagt gtccgcatga agttcacgat attgtaagcc
     3241 aataggctta cgatcatacg agcatgattt tctataaaac gtgggctatc tgttttatcc
     3301 aagtaaaagc cgttttttgc ttccttaatg taattttcca tcgttcctct ttttgaatac
     3361 gtttggaaaa ccatttctgg agagacgtct tttgaaaaat tagtaatgat gaattcatgt
     3421 ctaaaaagca attcactagc ttctctggtt gattttatgc agatccttct ggattttccc
     3481 catgactttg cttgataagt ggcgttatag taatgaactt ctttcttact ccaatcgtga
     3541 tgatccttaa tggtaacgaa ttgatcagcg attctgtaca gattacgatt ggatttcaaa
     3601 cggatagtat acaaactatc gtactcttca caaagctcat acaaatcagg cattgcgaat
     3661 ccactatctg cacgtaccat aatagtgctt actggtgtca cagagtgata atgctcaaat
     3721 aatggacgag taaatgctgc gacgcctttt gaggtgtaga catttccaga acgaagttct
     3781 gctttaagaa agtctccggt aagaccatca aaggcaacga gtggatgata accggttgtc
     3841 tggtaatgcg cattataatc ggtcatttct tggtttccaa aggtatcact gtgagtagaa
     3901 tctaaatcga aaatcatttc gttcgtattt cgaatcgtcc gtgctttatc aatcaaaatc
     3961 tggttaacct tttgtagctg gacgatattt tctgttgata tccgatccca aaagcgagaa
     4021 atggatgact gtgaagccaa ccgctttttg cttaaaacag cttgaaagat aggatccgtt
     4081 gctaatatat tggctgaaga atctgtagga tagcctgcaa tcaactgcaa gatgatttgt
     4141 tccagaatag attcattttc atgaacatga tacagtcggt catcttgaat ctggatgttt
     4201 ttacataaga tttggctaaa ctcaaatttg ttcatgaatt cttttgctaa tattaagccg
     4261 gaatctgagg acaaatttcc accagagtga gatactgtaa catttgaatt gaaaagtaga
     4321 cgattttcgt gtaaagttgc cattgagaga acccctttct ttggttaggt ttagtcactt
     4381 taaccatagc agattggggt tctttttgta cacccaaagg gtgcgaataa acgaaacaaa
     4441 atagacagtt ataacaaggt ttttgaaacg tttttgaaaa actatgaatt attcaggatg
     4501 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
     4561 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
     4621 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
     4681 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
     4741 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
     4801 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
     4861 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     4921 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     4981 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     5041 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     5101 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     5161 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     5221 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     5281 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     5341 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     5401 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     5461 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     5521 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     5581 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     5641 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     5701 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     5761 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     5821 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     5881 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     5941 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     6001 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     6061 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     6121 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     6181 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     6241 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     6301 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     6361 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taatttttta
     6421 ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa
     6481 cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga
     6541 gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag
     6601 ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg
     6661 cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca
     6721 acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata
     6781 aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt
     6841 ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca
     6901 ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc
     6961 ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt
     7021 tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc
     7081 agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata
     7141 gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg
     7201 atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac
     7261 aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag
     7321 atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg
     7381 tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc
     7441 aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt
     7501 ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg
     7561 aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag
     7621 gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa
     7681 tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct
     7741 tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg
     7801 cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt
     7861 tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc
     7921 ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg
     7981 acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa
     8041 gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca
     8101 ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa
     8161 tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc
     8221 ggttgtgcgg tattgggaaa cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg
     8281 ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac
     8341 gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg
     8401 gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta
     8461 caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac
     8521 agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc
     8581 ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg
     8641 aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa
     8701 aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc
     8761 ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt
     8821 accgtcctaa gcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc
     8881 tgacaaagga aagttattat cccaatattg accgaactga gatgatttca aaaggatacg
     8941 tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag
     9001 acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc
     9061 atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca
     9121 tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg
     9181 aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg
     9241 acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg
     9301 gaatttatat atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat
     9361 ctgcgtgcgc tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt
     9421 tcacaccgcc cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt
     9481 atgaaaattc atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt
     9541 aatttacggg gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc
     9601 tagaaatatt tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg
     9661 tttttttatt gttattgtta ttgttattct taatatactt aggttatgac tacgttaatg
     9721 aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac
     9781 atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg
     9841 tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga
     9901 agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg
     9961 atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg
    10021 ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc
    10081 aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc
    10141 ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg
    10201 aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg
    10261 acaaaacaga gttaacagga attcaatatg aaccatggca tattcgctat gttggtttac
    10321 cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa
    10381 aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc
    10441 ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa
    10501 acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga
    10561 ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt
    10621 ttggggtaac gttagctttt taatttaaaa cccacgttaa ctaggacatt gctatactaa
    10681 tgatacaact taaacaaaag aattagagga aattatattg ggaaaaatat tatctagagg
    10741 attgctagct ttatatttag tgacactaat ctggttagtg ttattcaaat tacaatacaa
    10801 tattttatca gtatttaatt atcatcaaag aagtcttaac ttgactccat ttactgctac
    10861 tgggaatttc agagagatga tagataatgt tataatcttt attccatttg gcttgctttt
    10921 gaatgtcaat tttaaagaaa tcggattttt acctaagttt gcttttgtac tggttttaag
    10981 tcttactttt gaaataattc aatttatctt cgctattgga gcgacagaca taacagatgt
    11041 aattacaaat actgttggag gctttcttgg actgaaatta tatggtttaa gcaataagca
    11101 tatgaatcaa aaaaaattag acagagttat tatttttgta ggtatacttt tgctcgtatt
    11161 attgctcgtt taccgtaccc atttaagaat aaattacgtg taagatgtct aaatcaagca
    11221 atctgatctt tcatacacat aaagatattg aatgaattgg attagatgga aaacgggatg
    11281 tggggaaact cgcccgtagg tgtgaagtga ggggaaaacc ggtgataaag taaaaagctt
    11341 acctaacact atagtaacaa agaaagccca attatcaatt ttagtgctga ggaattggtc
    11401 tctttaataa atttccttaa cgttgtaaat ccgcattttc ctgacggtac ccctacgaga
    11461 gcgattttga gccattttca aacgtaatat tgaataacta tccattaaaa tccttacaca
    11521 gcacgattta aagtagacaa agaaaataaa aggaaagccc tgctaatgaa atttattagc
    11581 agggcttttt agtaagaaaa tatttgaacc atttttacag atttagccta tacgtccttt
    11641 cagttatttt taagtgcgta tatgtcgttt gcttttagcg ttatcgcaaa taagaaccta
    11701 ttcctgcaag gg
//
LOCUS       DACECB010000223        10048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.088, whole genome shotgun sequence.
ACCESSION   DACECB010000223 DACECB010000000
VERSION     DACECB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.088"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..93)
                     /locus_tag="IWO04_002917"
     CDS             complement(<1..93)
                     /locus_tag="IWO04_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0214431.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDH"
     gene            complement(311..1651)
                     /locus_tag="IWO04_002918"
     CDS             complement(311..1651)
                     /locus_tag="IWO04_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0214432.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1920..2816
                     /locus_tag="IWO04_002919"
     CDS             1920..2816
                     /locus_tag="IWO04_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0214433.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2966..3907)
                     /locus_tag="IWO04_002920"
     CDS             complement(2966..3907)
                     /locus_tag="IWO04_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0214434.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4033..5154
                     /locus_tag="IWO04_002921"
     CDS             4033..5154
                     /locus_tag="IWO04_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0214435.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5412..6914
                     /locus_tag="IWO04_002922"
     CDS             5412..6914
                     /locus_tag="IWO04_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0214436.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6981..7508
                     /locus_tag="IWO04_002923"
     CDS             6981..7508
                     /locus_tag="IWO04_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0214437.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7719..8780
                     /locus_tag="IWO04_002924"
     CDS             7719..8780
                     /locus_tag="IWO04_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0214438.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8783..9679
                     /gene="murQ"
                     /locus_tag="IWO04_002925"
     CDS             8783..9679
                     /gene="murQ"
                     /locus_tag="IWO04_002925"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0214439.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            9693..>10048
                     /locus_tag="IWO04_002926"
     CDS             9693..>10048
                     /locus_tag="IWO04_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAQ0214440.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEM"
BASE COUNT         3430 a         1808 c         2072 g         2738 t
ORIGIN      
        1 atgatcgagc gcatagacat tcaagtacat cataccaaac ataactaccg ttgaaacacc
       61 gatcatcaac aaaaacttcc aatacttttt catttaaaac aaactccttt ttttcattcc
      121 tttcttctat catgtcatga gcatggagaa caatcaatta ttttgcacag agggtttttg
      181 aacaccgttt accaagaggt catgagactg tttattcgga aaaaggagtt gcaacaagac
      241 ttttgtcaca actcctttct tcaatagctc catctattcc acataaataa aataaaattt
      301 tctatcgatt tcattcattc gtcaatacga acgcatcatt ctttttttga taactgaaat
      361 aaatgaacag gtaaagcagg ccagtcaata cagttacccc aatggagttc gtgaacagga
      421 taccaaggaa aatcaatcct aagacagcta atgcaaaaaa gctgattcct ttatcccata
      481 atgttccttt tcttagtgcc agtacaaagg atgccgcact gataatgatc cagatcaata
      541 atactgtata cgaaagggaa cctgccagat agccaaataa tttgtcaccg acaaaataag
      601 aaagagctac accaagataa agaacactgg tacaaaataa aacagcacgt tgcggtactt
      661 gatgcttgtt taaaacagct agttttctag aggaaccttt tcgatccttc aaacggaaga
      721 acaaaattct tgaagatgca tacacacccg aattgataga tgagaacaaa gcaaggacaa
      781 tcacaaaatt gacgatatcc gctgcaaatg ggatattcat tttttcaaat accatcacaa
      841 aaggactgac attcgtgcct gctaaagtat tccaaggaaa aagaatcaaa agcaaaaaca
      901 aaggaatgat gtaaaaggaa atgattcttc ccatgacacc tttgattgct ttaggaatcg
      961 cacgtttagg atcatctgct tcgcttaccg tgatagcaat cagttcagaa ccgccgtaag
     1021 aatagataac tacaagcaaa gaactgatga ttcccttcat accatgagga gtaaacccac
     1081 catggtcagt caattgctga agggtaggga aaacgcctgt gcccaacatt tgctgaccga
     1141 caagataagt agcaaaaatg atcagtaaga taatgacact gattttagca aaagccagcc
     1201 aatattctgt ttctgcgaat aaccgtaccg aataaagatt gatgatcgta gttaaaacag
     1261 ccaacataaa aacaaatacc caggctggaa tgtgcgggaa ccacaactgc aaaaaggaag
     1321 cagctgcaac agcttcagcg atgatattga taatccacat agaccagtag acccaatcag
     1381 taaaatcagc agaatgagca cctagataag ggcgtatcaa accagataat ccgtgatgcg
     1441 tgtcgccacc gcttaaaacg atttttccca gacctttcat cacgatatat aaaatcagcc
     1501 caccaatcaa aaaagcaatc aatacagaag ggccggcgat cccaatcgct tcgccacttc
     1561 ctttaaatag acctgcaccg attgctccac ctaaagctaa catggtgata tgtctggagc
     1621 taagttgttt tttcaatttt ttatcttcca taaatgacct ctttctttta aattcacaat
     1681 caaaatttat attaagaata aatagcaata tttaacaatg actaatgata acaaattttc
     1741 agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt
     1801 catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct
     1861 ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga
     1921 tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca
     1981 tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag
     2041 ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg
     2101 agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc
     2161 acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg
     2221 agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat
     2281 tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg
     2341 caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag
     2401 atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta
     2461 cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg
     2521 agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag
     2581 tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc
     2641 ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc
     2701 tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg
     2761 attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa
     2821 cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga
     2881 gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt
     2941 cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga
     3001 ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc
     3061 aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt
     3121 acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt
     3181 acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata
     3241 cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat
     3301 ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat
     3361 aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt
     3421 tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta
     3481 acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga
     3541 tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac
     3601 acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt
     3661 gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt
     3721 ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc
     3781 catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc
     3841 cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga
     3901 ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga
     3961 agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag
     4021 tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca
     4081 tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat
     4141 gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga
     4201 acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta
     4261 ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta
     4321 gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga
     4381 gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga
     4441 agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg
     4501 tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt
     4561 cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa
     4621 caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc
     4681 ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta
     4741 gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga
     4801 gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc
     4861 gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga
     4921 aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt
     4981 tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt
     5041 attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa
     5101 acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca
     5161 aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca
     5221 ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga
     5281 agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg
     5341 tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga
     5401 ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag
     5461 gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta
     5521 tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt
     5581 atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata
     5641 aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg
     5701 aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac
     5761 tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt
     5821 tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg
     5881 cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg
     5941 atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga
     6001 atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg
     6061 aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt
     6121 ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta
     6181 gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag
     6241 caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg
     6301 aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg
     6361 tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg
     6421 agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc
     6481 attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc
     6541 tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg
     6601 cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg
     6661 aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg
     6721 aattggctaa atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt
     6781 atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca
     6841 tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc
     6901 ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg
     6961 ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg
     7021 cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg
     7081 cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt
     7141 cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc
     7201 tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca
     7261 aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt
     7321 cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag
     7381 aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc
     7441 atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt
     7501 atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta
     7561 gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat
     7621 gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat atactctagt
     7681 catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta
     7741 tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct
     7801 ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc
     7861 gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt
     7921 caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa
     7981 ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt
     8041 cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat
     8101 tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc
     8161 ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag
     8221 gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag
     8281 gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat
     8341 tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg
     8401 agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg
     8461 aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg
     8521 tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag
     8581 ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg
     8641 agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag
     8701 ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta
     8761 taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg
     8821 cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat
     8881 gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc
     8941 agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc
     9001 cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt
     9061 agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt
     9121 cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac
     9181 aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg
     9241 attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc
     9301 cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac
     9361 aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac
     9421 tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac
     9481 aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta
     9541 cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat
     9601 gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt
     9661 tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag
     9721 aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc
     9781 actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat
     9841 taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg
     9901 gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag
     9961 gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc
    10021 gtgtaaacga acgggcacaa gaaatgaa
//
LOCUS       DACECB010000224         5878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.103, whole genome shotgun sequence.
ACCESSION   DACECB010000224 DACECB010000000
VERSION     DACECB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.103"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            219..884
                     /locus_tag="IWO04_002927"
                     /pseudo
     CDS             219..884
                     /locus_tag="IWO04_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            920..1165
                     /locus_tag="IWO04_002928"
     CDS             920..1165
                     /locus_tag="IWO04_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0214441.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1268..2137
                     /locus_tag="IWO04_002929"
     CDS             1268..2137
                     /locus_tag="IWO04_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0214442.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2118..2852
                     /locus_tag="IWO04_002930"
     CDS             2118..2852
                     /locus_tag="IWO04_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0214443.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2885..3793
                     /locus_tag="IWO04_002931"
     CDS             2885..3793
                     /locus_tag="IWO04_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0214444.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3790..4332
                     /gene="sat4"
                     /locus_tag="IWO04_002932"
     CDS             3790..4332
                     /gene="sat4"
                     /locus_tag="IWO04_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0214445.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4425..5219
                     /locus_tag="IWO04_002933"
                     /note="aph(3')-IIIa"
     CDS             4425..5219
                     /locus_tag="IWO04_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0214446.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5327..5515)
                     /locus_tag="IWO04_002934"
                     /pseudo
     CDS             complement(5327..5515)
                     /locus_tag="IWO04_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5495..5839
                     /locus_tag="IWO04_002935"
                     /pseudo
     CDS             5495..5839
                     /locus_tag="IWO04_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         2012 a          872 c         1287 g         1707 t
ORIGIN      
        1 ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca aaataaacat
       61 ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag
      121 tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata
      181 atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa
      241 accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg
      301 ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta
      361 tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc
      421 aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct
      481 acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga
      541 ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg
      601 gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata
      661 aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag
      721 cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt
      781 taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg
      841 aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta
      901 ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga
      961 aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt
     1021 ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt
     1081 tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta
     1141 taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg
     1201 ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc
     1261 atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta
     1321 tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga
     1381 tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt
     1441 cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg
     1501 tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt
     1561 acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc
     1621 caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc
     1681 gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga
     1741 aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt
     1801 ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca
     1861 tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg
     1921 tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata
     1981 taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga
     2041 catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt
     2101 aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat
     2161 acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga
     2221 aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat
     2281 ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc
     2341 ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct
     2401 gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg
     2461 cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg
     2521 ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag
     2581 actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg
     2641 agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga
     2701 ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac
     2761 agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga
     2821 tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat
     2881 gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga
     2941 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
     3001 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
     3061 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
     3121 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
     3181 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
     3241 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
     3301 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
     3361 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
     3421 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
     3481 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
     3541 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
     3601 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
     3661 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
     3721 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     3781 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     3841 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     3901 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     3961 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttac
     4021 taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct
     4081 tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt
     4141 atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc
     4201 caggacaata accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc
     4261 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     4321 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     4381 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     4441 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     4501 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     4561 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     4621 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     4681 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     4741 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     4801 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     4861 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     4921 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     4981 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     5041 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     5101 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     5161 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     5221 acctagattt agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc
     5281 tgaagtacat ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta
     5341 gatagagttc tatattagag atgtaaagag ttttggtgaa gaggtttgtg agtatggctt
     5401 ttataaccag ggtgcaatga gaagcactaa cagcagctag cttaagaacg cataggatat
     5461 actttttatg gaagtcacag aggaggaata aaatttgaac aggattaaca gagtaacctc
     5521 tattcttatt cagctgcaat caaaaaaaat aattcctgcc aaagaaattg cacagcgatt
     5581 taatataagc ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc
     5641 aattggatct gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt
     5701 aatgtttaca gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca
     5761 tggagatgaa tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt
     5821 gaagcatggt gaaaaaaacg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DACECB010000225         4368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.117, whole genome shotgun sequence.
ACCESSION   DACECB010000225 DACECB010000000
VERSION     DACECB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.117"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            319..1797
                     /gene="msr"
                     /locus_tag="IWO04_002936"
     CDS             319..1797
                     /gene="msr"
                     /locus_tag="IWO04_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0214447.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1844..2290
                     /locus_tag="IWO04_002937"
     CDS             1844..2290
                     /locus_tag="IWO04_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0214448.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2489..3004)
                     /locus_tag="IWO04_002938"
     CDS             complement(2489..3004)
                     /locus_tag="IWO04_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0214449.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3121..4322
                     /locus_tag="IWO04_002939"
                     /pseudo
     CDS             3121..4322
                     /locus_tag="IWO04_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1314 a          732 c          946 g         1376 t
ORIGIN      
        1 ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg
       61 aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa
      121 caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat
      181 gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag
      241 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
      301 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
      361 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
      421 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
      481 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
      541 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
      601 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
      661 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
      721 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
      781 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
      841 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
      901 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
      961 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
     1021 aagcaacaaa tcagaccgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
     1081 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
     1141 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
     1201 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
     1261 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
     1321 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
     1381 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
     1441 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
     1501 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
     1561 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
     1621 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
     1681 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
     1741 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
     1801 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
     1861 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
     1921 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
     1981 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
     2041 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
     2101 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
     2161 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
     2221 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
     2281 taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc
     2341 tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg
     2401 atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag
     2461 atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta
     2521 cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat
     2581 aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt
     2641 gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat
     2701 cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag
     2761 tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct
     2821 ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg
     2881 caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca
     2941 attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat
     3001 tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt
     3061 tttacatgtg catatttaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg
     3121 atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg
     3181 aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta
     3241 gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc
     3301 ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta
     3361 tctagtcgga tcggtttgaa aaaacattga tgacaggatt gtttatcatc gttgtattcg
     3421 ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg
     3481 gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg
     3541 gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg
     3601 gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag
     3661 catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac
     3721 caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt
     3781 cagttggact aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc
     3841 catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg
     3901 tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt
     3961 atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg
     4021 gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa
     4081 ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg
     4141 cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag
     4201 gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc
     4261 ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat
     4321 aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DACECB010000226         4323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.118, whole genome shotgun sequence.
ACCESSION   DACECB010000226 DACECB010000000
VERSION     DACECB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.118"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002940"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0214450.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     CPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO04_002941"
     CDS             2039..2227
                     /locus_tag="IWO04_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0214451.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWO04_002942"
     CDS             complement(2285..2638)
                     /locus_tag="IWO04_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0214452.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWO04_002943"
     CDS             3169..3591
                     /locus_tag="IWO04_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0214453.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWO04_002944"
     CDS             3588..3818
                     /locus_tag="IWO04_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214454.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         1447 a          705 c          832 g         1339 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtgtcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aac
//
LOCUS       DACECB010000227         4323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.119, whole genome shotgun sequence.
ACCESSION   DACECB010000227 DACECB010000000
VERSION     DACECB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.119"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002945"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0214455.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     CPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO04_002946"
     CDS             2039..2227
                     /locus_tag="IWO04_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0214456.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWO04_002947"
     CDS             complement(2285..2638)
                     /locus_tag="IWO04_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0214457.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWO04_002948"
     CDS             3169..3591
                     /locus_tag="IWO04_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0214458.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWO04_002949"
     CDS             3588..3818
                     /locus_tag="IWO04_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0214459.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         1446 a          705 c          833 g         1339 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtgtcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aac
//
LOCUS       DACECB010000228         4146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.123, whole genome shotgun sequence.
ACCESSION   DACECB010000228 DACECB010000000
VERSION     DACECB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.123"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002950"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0214460.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     CPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWO04_002951"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWO04_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0214461.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..>4146
                     /locus_tag="IWO04_002952"
     CDS             3976..>4146
                     /locus_tag="IWO04_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0214462.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNER"
BASE COUNT         1331 a          633 c          854 g         1328 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtgtcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgt
//
LOCUS       DACECB010000229         4146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.124, whole genome shotgun sequence.
ACCESSION   DACECB010000229 DACECB010000000
VERSION     DACECB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.124"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002953"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO04_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0214463.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     CPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWO04_002954"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWO04_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0214464.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..>4146
                     /locus_tag="IWO04_002955"
     CDS             3976..>4146
                     /locus_tag="IWO04_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0214465.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNER"
BASE COUNT         1330 a          633 c          855 g         1328 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtgtcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgt
//
LOCUS       DACECB010000230         3272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.131, whole genome shotgun sequence.
ACCESSION   DACECB010000230 DACECB010000000
VERSION     DACECB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.131"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..728
                     /locus_tag="IWO04_002956"
     CDS             <1..728
                     /locus_tag="IWO04_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0214466.1"
                     /translation="AYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERF
                     LFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRS
                     FPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPK
                     IQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDV
                     SETEAQLEEKMARWEYLSEWVED"
     gene            740..1687
                     /locus_tag="IWO04_002957"
     CDS             740..1687
                     /locus_tag="IWO04_002957"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0214467.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1707..2234
                     /locus_tag="IWO04_002958"
     CDS             1707..2234
                     /locus_tag="IWO04_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0214468.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2297..2950)
                     /locus_tag="IWO04_002959"
     CDS             complement(2297..2950)
                     /locus_tag="IWO04_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0214469.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            3082..>3272
                     /locus_tag="IWO04_002960"
     CDS             3082..>3272
                     /locus_tag="IWO04_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EDD domain protein"
                     /protein_id="HAQ0214470.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTS"
BASE COUNT         1166 a          580 c          678 g          848 t
ORIGIN      
        1 ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga taaacggatg attgcttact
       61 tacaagaagc tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga gtagcagaat
      121 tacttgaacg atttttattt ccaagattta tgcatgggac actgataggc aaactttcag
      181 gcggagaaaa acgccggtta tttttgttga aactgctgat tcagcaacct aatgttttat
      241 tattggatga accgaccaat gatttggaca tagcaacatt gacgattttg gaagattatt
      301 ttagaagttt tccaggtgct gtgatcactg tttcccatga ccgttatttc ttggacaagg
      361 tagcagataa gttattagta ttccaaggaa acggaaaaca agaactttat tacggaaaca
      421 tgtccagtta tttactgaag caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa
      481 aaatacagag caaggaacct gctgaaaaga agaaattatc ttacatggaa caaaaagaat
      541 gggaaacgat tgaagatgag attgcagaac ttgaagaaaa aatctcattg ctccaagaag
      601 agatgaatca tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg
      661 aaacagaagc acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag
      721 aagattagga ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga
      781 agaagggcat cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca
      841 aatgcggttt gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg
      901 attgatcaaa agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt
      961 acaaaataaa aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga
     1021 ctataatgga ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa
     1081 tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt
     1141 tgctgaaaca tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac
     1201 aaaagacggt ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc
     1261 tgattctaga agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt
     1321 gcctccttgt catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt
     1381 gtatcaacgc agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt
     1441 gctgactcac ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt
     1501 aggagatgct catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga
     1561 aatacgcgca tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga
     1621 gatggaagat atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc
     1681 tgtttagaat cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg
     1741 gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca
     1801 aacaaatgac agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta
     1861 gccgtccgtt gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag
     1921 aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg
     1981 gtatcttttt catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga
     2041 tattataccg tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg
     2101 actggtctga ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt
     2161 atccgcatca atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg
     2221 aattgacgat ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat
     2281 agctttattt tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta
     2341 acataacaaa caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga
     2401 atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa
     2461 gagagtgata aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga
     2521 tcgtagatac ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc
     2581 caagaatcgc aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac
     2641 tcagcaaaca aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga
     2701 atacttggaa cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta
     2761 aaaataaaag aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat
     2821 gcaagtgggc accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa
     2881 ttccatgggt gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg
     2941 ttttttccat gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct
     3001 gttatactaa aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta
     3061 aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg
     3121 atggaaaaaa atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc
     3181 gacggaacag tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg
     3241 gcgaaagcaa aaaatctgcc caaaacaagc ca
//
LOCUS       DACECB010000231         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.143, whole genome shotgun sequence.
ACCESSION   DACECB010000231 DACECB010000000
VERSION     DACECB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.143"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IWO04_002961"
     CDS             24..416
                     /locus_tag="IWO04_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0214471.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..1856
                     /locus_tag="IWO04_002962"
     CDS             417..1856
                     /locus_tag="IWO04_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0214472.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1986..>2232)
                     /locus_tag="IWO04_002963"
     CDS             complement(1986..>2232)
                     /locus_tag="IWO04_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0214473.1"
                     /translation="FDSRFKTLQQNPFQYTVQGNSHNRLKSTNLIERLNQEVRRREKI
                     IRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          895 a          189 c          381 g          767 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta tggaattaaa aatattaaac
     1801 aggaatttat cgaaaatggt agaaaagaaa tttataaaag gacttataaa gattgattat
     1861 ataatatatg aaaagctatt ataaaagaca ttagtattaa atagtttaaa aaaatgaaaa
     1921 ataataaagg aagtgagtca attccagact cctgtgtaaa atgctataca atgtttttac
     1981 catttctact tatcaaaatt gatgtatttt cttgaagaat aaatccattc atcatgtagg
     2041 tccataagaa cggctccaat taagcgattg gctgatgttt gattggggaa gatgcgaata
     2101 atcttttctc ttctgcgtac ttcttgattc agtcgttcaa ttagattggt actctttagt
     2161 cgattgtggg aatttccttg tacggtatat tgaaaggggt tctgttgcaa agttttaaat
     2221 ctactatcaa at
//
LOCUS       DACECB010000232         1706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.153, whole genome shotgun sequence.
ACCESSION   DACECB010000232 DACECB010000000
VERSION     DACECB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.153"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            190..459
                     /locus_tag="IWO04_002964"
     CDS             190..459
                     /locus_tag="IWO04_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0214474.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVQYLKILCIIGIEVKLDAKNL"
     gene            480..563
                     /locus_tag="IWO04_002965"
     CDS             480..563
                     /locus_tag="IWO04_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0214475.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            688..1425
                     /gene="erm(B)"
                     /locus_tag="IWO04_002966"
     CDS             688..1425
                     /gene="erm(B)"
                     /locus_tag="IWO04_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0214476.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1571..>1706
                     /locus_tag="IWO04_002967"
     CDS             1571..>1706
                     /locus_tag="IWO04_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ0214477.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFR
                     K"
BASE COUNT          651 a          259 c          296 g          500 t
ORIGIN      
        1 ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca aaataaacat
       61 aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca caaatcactt
      121 atcacaaatc acaagtgatt tgtgattgtt gatgataaaa taagaataag aagaaataga
      181 aagaagtgag tgattgtggg aaatttaggc gcacaaaaag aaaaacgaaa tgatacacca
      241 atcagtgcaa aaaaagatat aatgggagat aagacggttc gtgttcgtgc tgacttgcac
      301 catatcataa aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggaaata
      361 agacttagaa gcaaacttaa gagtgtgttg atagtgcagt atcttaaaat tttgtgtata
      421 ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca
      481 tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa
      541 acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat
      601 gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac
      661 agatgtaatc acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt
      721 ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat
      781 accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt
      841 aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc agaaaaatta
      901 aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac
      961 aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa
     1021 aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac
     1081 aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt
     1141 cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc
     1201 ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa gctatatacg
     1261 tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt
     1321 catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta
     1381 ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt
     1441 ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat tagatatact
     1501 actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata
     1561 aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg
     1621 caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt
     1681 tactttagct gaacatttta gaaaga
//
LOCUS       DACECB010000233         1380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.164, whole genome shotgun sequence.
ACCESSION   DACECB010000233 DACECB010000000
VERSION     DACECB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.164"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..214)
                     /locus_tag="IWO04_002968"
     CDS             complement(<1..214)
                     /locus_tag="IWO04_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0214478.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGN"
     gene            767..1315
                     /gene="aac(6')"
                     /locus_tag="IWO04_002969"
     CDS             767..1315
                     /gene="aac(6')"
                     /locus_tag="IWO04_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0214479.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          466 a          222 c          262 g          430 t
ORIGIN      
        1 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
       61 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
      121 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
      181 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
      241 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
      301 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
      361 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
      421 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
      481 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
      541 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
      601 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      661 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      721 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      781 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      841 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      901 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      961 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
     1021 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
     1081 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
     1141 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
     1201 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
     1261 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
     1321 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACECB010000234          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41201_2017
            SAMN12639969-rid9880123.guided.183, whole genome shotgun sequence.
ACCESSION   DACECB010000234 DACECB010000000
VERSION     DACECB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639969
            Sequence Read Archive: SRR10023251
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741776.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:23:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,970
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,834
            CDSs (with protein)               :: 2,834
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 28 of 73
            Pseudo Genes (internal stop)      :: 20 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639969-rid9880123.guided.183"
                     /isolate="E41201_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            275..772
                     /gene="dfrG"
                     /locus_tag="IWO04_002970"
     CDS             275..772
                     /gene="dfrG"
                     /locus_tag="IWO04_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0214480.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          334 a           90 c          168 g          272 t
ORIGIN      
        1 taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt
       61 tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg
      121 ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat
      181 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
      241 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      301 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      361 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      421 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      481 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      541 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      601 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      661 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      721 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      781 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      841 ttgtcatttg aaataaatta tatc
//
